directory filename parent_charge numPSMsObserved numLCrunsObserved score rank2Score deltaForwardReverseScore deltaRank1Rank2Score percent_scored_peak_intensity backbone_cleavage_score missedCleavages numKorR totalIntensity variableSites nterm StartAA previous_aa sequenceLength sequence next_aa sequenceLengthProt sequenceMulti rank2Sequence sequenceMap recallMstag numCoveredAAsDB numNcuts numCcuts numNCcuts numIcuts fragmentationCategory cys modifications precursorAveragineChiSquared precursorIsolationPurityPercent retentionTimeMin trapFillMsec faimsCV IonMobility_1_over_K0 parent_m_over_z matched_parent_mass delta_parent_mass delta_parent_mass_ppm delta_ppm_norm peptide_pI protein_mw species accession_number accession_numbers geneSymbol_smorf smorfType genomeCoordinates_smorf chromosome_smorf geneSymbol_nuORFdb mergeType plotType entry_name runname 40mio-rep1 E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep1_Slot1-04_1_3496.15542.542.1 1 1 1 7.03 5.65 1.88 1.38 68.8 7 0 0 3720.0 Hydrogen 2 (M) 11 AAAAAAAAAWA (R) 11 AAAAAAAAAAnA A A A A\A\A\A\A\A\W\A 63.6 7 7 0 0 0 b-ion bias 53.686657 1.400957 915.4632 915.468 -0.0053 -5.8 -2.5999999999999996 5.57 1591.8 smORF_v4|nuORFdb_human PRELID1_5:176730817-176730870:+ PRELID1_5:176730817-176730870:+|ENST00000510797.1_1_5:176730816-176730870:+ PRELID1 uORF 5:176730817-176730870:+ 5 PRELID1 5' uORF 5' uORF GN=PRELID1 E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep1_Slot1-04_1_3496 40mio-rep2 E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep2_Slot2-5_1_3520.10995.697.1 1 1 1 10.27 7.72 2.56 2.54 81.9 6 0 0 3920.0 Hydrogen 10 (A) 9 AAAAAAEAW (L) 9 VNAENVW A/A/A|A|A A-E\A\W 77.8 7 4 4 2 1 unbiased 37.983074 1.30812 831.3952 831.399 -0.0045 -5.5 -0.5999999999999996 4.0 12249.5 nuORFdb_human ENST00000451837.5_1_22:24037326-24037671:- ENST00000451837.5_1_22:24037326-24037671:- GUSBP11 ncRNA Processed Transcript lncRNA GN=GUSBP11 ncRNA Processed Transcript E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep2_Slot2-5_1_3520 10mio-rep1 E_20221201_NO30_400nL_HLAc1_1e7_directIP_titration_rep1_Slot2-3_1_3512.11186.725.1 1 1 1 10.63 8.38 6.31 2.25 75.5 8 0 0 2640.0 Hydrogen 11 (T) 10 AAAAAGAASW (A) 10 AAAAQAASW A A/A/A/A|G\A\A|S\W 80.0 8 5 5 2 0 unbiased 38.648048 1.29139 846.4029 846.41 -0.0074 -8.8 -1.1000000000000005 5.57 6116.0 smORF_v4|nuORFdb_human URI1_19:30433277-30433438:+ URI1_19:30433277-30433438:+|ENST00000574110.5_1_19:30433210-30433438:+ URI1 uORF 19:30433277-30433438:+ 19 URI1 5' uORF 5' uORF GN=URI1 E_20221201_NO30_400nL_HLAc1_1e7_directIP_titration_rep1_Slot2-3_1_3512 10mio-rep2 E_20221201_NO30_400nL_HLAc1_1e7_directIP_titration_rep2_Slot2-3_1_3513.11152.722.1 1 2 1 10.24 7.88 5.26 2.36 76.1 8 0 0 4770.0 Hydrogen 11 (T) 10 AAAAAGAASW (A) 10 AAAAQAASW A A/A/A/A/G\A\A\S\W 80.0 8 4 4 0 0 unbiased 38.528458 1.293255 846.4011 846.41 -0.0094 -11.2 -4.499999999999999 5.57 6116.0 smORF_v4|nuORFdb_human URI1_19:30433277-30433438:+ URI1_19:30433277-30433438:+|ENST00000574110.5_1_19:30433210-30433438:+ URI1 uORF 19:30433277-30433438:+ 19 URI1 5' uORF 5' uORF GN=URI1 E_20221201_NO30_400nL_HLAc1_1e7_directIP_titration_rep2_Slot2-3_1_3513 20mio-rep1 E_20221201_NO30_400nL_HLAc1_2e7_directIP_titration_rep1_Slot2-4_1_3515.11078.725.1 1 1 1 12.18 9.94 5.42 2.24 81.8 8 0 0 1830.0 Hydrogen 11 (T) 10 AAAAAGAASW (A) 10 AAAAQAASW A A/A/A|A|G|A\A\S\W 80.0 8 6 5 3 0 unbiased 38.262932 1.290963 846.407 846.41 -0.0034 -4.1 2.1000000000000005 5.57 6116.0 smORF_v4|nuORFdb_human URI1_19:30433277-30433438:+ URI1_19:30433277-30433438:+|ENST00000574110.5_1_19:30433210-30433438:+ URI1 uORF 19:30433277-30433438:+ 19 URI1 5' uORF 5' uORF GN=URI1 E_20221201_NO30_400nL_HLAc1_2e7_directIP_titration_rep1_Slot2-4_1_3515 20mio-rep2 E_20221201_NO30_400nL_HLAc1_2e7_directIP_titration_rep3_Slot2-4_1_3518.11243.713.1 1 2 1 14.29 10.89 6.02 3.4 86.3 8 0 0 5790.0 Hydrogen 11 (T) 10 AAAAAGAASW (A) 10 AAAAQAASW A A/A/A|A|G\A\A\S\W 80.0 8 6 4 2 0 unbiased 38.834831 1.298412 846.4074 846.41 -0.0034 -4.1 2.2 5.57 6116.0 smORF_v4|nuORFdb_human URI1_19:30433277-30433438:+ URI1_19:30433277-30433438:+|ENST00000574110.5_1_19:30433210-30433438:+ URI1 uORF 19:30433277-30433438:+ 19 URI1 5' uORF 5' uORF GN=URI1 E_20221201_NO30_400nL_HLAc1_2e7_directIP_titration_rep3_Slot2-4_1_3518 20mio-rep3 E_20221201_NO30_400nL_HLAc1_2e7_directIP_titration_rep4_Slot2-4_1_3519.11095.713.1 1 1 1 9.9 8.07 3.18 1.83 77.6 6 0 0 3280.0 Hydrogen 11 (T) 10 AAAAAGAASW (A) 10 AAAAAAQSEG A A A/A A|G\A\A\S\W 50.0 5 5 2 1 0 b-ion bias 38.328697 1.298478 846.403 846.41 -0.0074 -8.8 -3.000000000000001 5.57 6116.0 smORF_v4|nuORFdb_human URI1_19:30433277-30433438:+ URI1_19:30433277-30433438:+|ENST00000574110.5_1_19:30433210-30433438:+ URI1 uORF 19:30433277-30433438:+ 19 URI1 5' uORF 5' uORF GN=URI1 E_20221201_NO30_400nL_HLAc1_2e7_directIP_titration_rep4_Slot2-4_1_3519 40mio-rep1 E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep1_Slot1-04_1_3496.11367.697.1 1 2 1 11.97 9.75 5.01 2.22 77.8 7 0 0 8340.0 Hydrogen 11 (T) 10 AAAAAGAASW (A) 10 AAAAQAASW A A A/A|A|G\A\A|S\W 70.0 7 6 4 3 0 unbiased 39.2663 1.30802 846.4064 846.41 -0.0044 -5.2 -2.0 5.57 6116.0 smORF_v4|nuORFdb_human URI1_19:30433277-30433438:+ URI1_19:30433277-30433438:+|ENST00000574110.5_1_19:30433210-30433438:+ URI1 uORF 19:30433277-30433438:+ 19 URI1 5' uORF 5' uORF GN=URI1 E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep1_Slot1-04_1_3496 40mio-rep2 E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep2_Slot2-5_1_3520.11073.717.1 1 1 1 12.71 9.73 4.69 2.99 83.9 7 0 0 3470.0 Hydrogen 11 (T) 10 AAAAAGAASW (A) 10 AAAAQAASW A A A/A|A|G|A\A\S\W 70.0 7 6 4 3 0 unbiased 38.252323 1.296148 846.4028 846.41 -0.0074 -8.8 -3.9000000000000004 5.57 6116.0 smORF_v4|nuORFdb_human URI1_19:30433277-30433438:+ URI1_19:30433277-30433438:+|ENST00000574110.5_1_19:30433210-30433438:+ URI1 uORF 19:30433277-30433438:+ 19 URI1 5' uORF 5' uORF GN=URI1 E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep2_Slot2-5_1_3520 40mio-rep3 E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep3_Slot2-5_1_3521.11067.709.1 1 2 1 12.72 11.06 5.35 1.66 81.4 7 0 0 4960.0 Hydrogen 11 (T) 10 AAAAAGAASW (A) 10 AAAAQAASW A A/A A/A\G|A|A|S\W 60.0 6 5 5 3 0 unbiased 38.234219 1.300751 846.4073 846.41 -0.0034 -4.1 0.5 5.57 6116.0 smORF_v4|nuORFdb_human URI1_19:30433277-30433438:+ URI1_19:30433277-30433438:+|ENST00000574110.5_1_19:30433210-30433438:+ URI1 uORF 19:30433277-30433438:+ 19 URI1 5' uORF 5' uORF GN=URI1 E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep3_Slot2-5_1_3521 40mio-rep4 E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep4_Slot2-5_1_3522.11082.714.1 1 1 1 11.5 9.2 5.12 2.3 81.4 8 0 0 5160.0 Hydrogen 11 (T) 10 AAAAAGAASW (A) 10 AAAAQAASW A A/A/A/A/G\A\A\S\W 80.0 8 4 4 0 0 unbiased 38.271408 1.297776 846.4029 846.41 -0.0074 -8.8 -4.1000000000000005 5.57 6116.0 smORF_v4|nuORFdb_human URI1_19:30433277-30433438:+ URI1_19:30433277-30433438:+|ENST00000574110.5_1_19:30433210-30433438:+ URI1 uORF 19:30433277-30433438:+ 19 URI1 5' uORF 5' uORF GN=URI1 E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep4_Slot2-5_1_3522 10mio-rep1 E_20221201_NO30_400nL_HLAc1_1e7_directIP_titration_rep1_Slot2-3_1_3512.10198.774.1 1 1 1 7.72 5.97 4.52 1.75 62.8 7 0 0 1470.0 Hydrogen 12 (A) 9 AAAAGAASW (A) 9 AAAQAASW A A/A/A/G\A\A|S\W 77.8 7 4 4 1 0 unbiased 35.234035 1.261502 775.3666 775.373 -0.0063 -8.2 -0.4999999999999991 5.57 6116.0 smORF_v4|nuORFdb_human URI1_19:30433277-30433438:+ URI1_19:30433277-30433438:+|ENST00000574110.5_1_19:30433210-30433438:+ URI1 uORF 19:30433277-30433438:+ 19 URI1 5' uORF 5' uORF GN=URI1 E_20221201_NO30_400nL_HLAc1_1e7_directIP_titration_rep1_Slot2-3_1_3512 20mio-rep1 E_20221201_NO30_400nL_HLAc1_2e7_directIP_titration_rep1_Slot2-4_1_3515.10087.778.1 1 1 1 10.81 8.96 5.47 1.85 78.6 7 0 0 2110.0 Hydrogen 12 (A) 9 AAAAGAASW (A) 9 AAAQAASW A A/A/A/G\A|A\S\W 77.8 7 4 4 1 0 unbiased 34.841484 1.259323 775.3677 775.373 -0.0053 -6.9 -0.7000000000000002 5.57 6116.0 smORF_v4|nuORFdb_human URI1_19:30433277-30433438:+ URI1_19:30433277-30433438:+|ENST00000574110.5_1_19:30433210-30433438:+ URI1 uORF 19:30433277-30433438:+ 19 URI1 5' uORF 5' uORF GN=URI1 E_20221201_NO30_400nL_HLAc1_2e7_directIP_titration_rep1_Slot2-4_1_3515 20mio-rep2 E_20221201_NO30_400nL_HLAc1_2e7_directIP_titration_rep3_Slot2-4_1_3518.10272.772.1 1 1 1 9.85 8.07 3.99 1.78 75.5 7 0 0 2770.0 Hydrogen 12 (A) 9 AAAAGAASW (A) 9 AAAQAASW A A/A/A/G|A\A\S\W 77.8 7 4 4 1 0 unbiased 35.48214 1.263061 775.3647 775.373 -0.0083 -10.7 -4.3999999999999995 5.57 6116.0 smORF_v4|nuORFdb_human URI1_19:30433277-30433438:+ URI1_19:30433277-30433438:+|ENST00000574110.5_1_19:30433210-30433438:+ URI1 uORF 19:30433277-30433438:+ 19 URI1 5' uORF 5' uORF GN=URI1 E_20221201_NO30_400nL_HLAc1_2e7_directIP_titration_rep3_Slot2-4_1_3518 20mio-rep3 E_20221201_NO30_400nL_HLAc1_2e7_directIP_titration_rep4_Slot2-4_1_3519.10118.769.1 1 1 1 12.06 9.67 5.2 2.39 81.8 7 0 0 3000.0 Hydrogen 12 (A) 9 AAAAGAASW (A) 9 AAAQAASW A A/A/A/G\A\A|S\W 77.8 7 4 4 1 0 unbiased 34.952316 1.264518 775.3682 775.373 -0.0053 -6.9 -1.1000000000000005 5.57 6116.0 smORF_v4|nuORFdb_human URI1_19:30433277-30433438:+ URI1_19:30433277-30433438:+|ENST00000574110.5_1_19:30433210-30433438:+ URI1 uORF 19:30433277-30433438:+ 19 URI1 5' uORF 5' uORF GN=URI1 E_20221201_NO30_400nL_HLAc1_2e7_directIP_titration_rep4_Slot2-4_1_3519 40mio-rep1 E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep1_Slot1-04_1_3496.10380.754.1 1 1 1 11.21 9.51 6.71 1.7 79.0 7 0 0 3460.0 Hydrogen 12 (A) 9 AAAAGAASW (A) 9 AAAQAASW A A/A/A/G|A\A\S\W 77.8 7 4 4 1 0 unbiased 35.858402 1.27364 775.3686 775.373 -0.0043 -5.6 -2.3999999999999995 5.57 6116.0 smORF_v4|nuORFdb_human URI1_19:30433277-30433438:+ URI1_19:30433277-30433438:+|ENST00000574110.5_1_19:30433210-30433438:+ URI1 uORF 19:30433277-30433438:+ 19 URI1 5' uORF 5' uORF GN=URI1 E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep1_Slot1-04_1_3496 40mio-rep2 E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep2_Slot2-5_1_3520.10093.765.1 1 1 1 11.53 9.37 5.49 2.16 74.2 7 0 0 4610.0 Hydrogen 12 (A) 9 AAAAGAASW (A) 9 AAAQAASW A A/A/A|G\A|A|S\W 77.8 7 5 5 3 0 dual b/y bias 34.866108 1.266776 775.3685 775.373 -0.0043 -5.6 -0.6999999999999993 5.57 6116.0 smORF_v4|nuORFdb_human URI1_19:30433277-30433438:+ URI1_19:30433277-30433438:+|ENST00000574110.5_1_19:30433210-30433438:+ URI1 uORF 19:30433277-30433438:+ 19 URI1 5' uORF 5' uORF GN=URI1 E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep2_Slot2-5_1_3520 40mio-rep4 E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep4_Slot2-5_1_3522.10099.764.1 1 2 1 10.76 8.47 5.04 2.29 75.9 7 0 0 8900.0 Hydrogen 12 (A) 9 AAAAGAASW (A) 9 AAAQAASW A A/A/A|G\A\A\S\W 77.8 7 5 3 1 0 unbiased 34.878201 1.267366 775.3696 775.373 -0.0033 -4.3 0.40000000000000036 5.57 6116.0 smORF_v4|nuORFdb_human URI1_19:30433277-30433438:+ URI1_19:30433277-30433438:+|ENST00000574110.5_1_19:30433210-30433438:+ URI1 uORF 19:30433277-30433438:+ 19 URI1 5' uORF 5' uORF GN=URI1 E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep4_Slot2-5_1_3522 40mio-rep1 E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep1_Slot1-04_1_3496.7063.1515.2 2 1 1 8.8 6.22 4.58 2.59 62.0 6 1 1 5740.0 Hydrogen 32 (R) 9 AAADARNPL (G) 9 AAAnGRQPL A/A/A/D|A/R N\P L 55.6 5 2 5 1 0 y-ion bias 24.405468 0.803955 449.7376 898.474 -0.0054 -6.0 -2.8 5.88 5170.8 smORF_v4|nuORFdb_human PCIF1_20:44563347-44566103:+ PCIF1_20:44563347-44566103:+|ENST00000372409.7_1_20:44563346-44566100:+ PCIF1 uORF 20:44563347-44566103:+ 20 PCIF1 5' uORF 5' uORF GN=PCIF1 E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep1_Slot1-04_1_3496 20mio-rep1 E_20221201_NO30_400nL_HLAc1_2e7_directIP_titration_rep1_Slot2-4_1_3515.17193.629.1 1 1 1 10.8 4.96 5.94 5.84 81.7 6 0 0 2270.0 Hydrogen 5 (A) 9 AAAGAQLSW (A) 9 AAAAANLDSA A A/A/G A|Q\L\S\W 55.6 5 4 3 1 0 unbiased 59.379047 1.34884 874.4394 874.442 -0.0027 -3.1 3.1 5.57 2511.8 nuORFdb_human ENST00000596974.5_1_19:47249325-47251339:+ ENST00000596974.5_1_19:47249325-47251339:+ FKRP 5' uORF 5' uORF GN=FKRP 5' uORF E_20221201_NO30_400nL_HLAc1_2e7_directIP_titration_rep1_Slot2-4_1_3515 20mio-rep3 E_20221201_NO30_400nL_HLAc1_2e7_directIP_titration_rep4_Slot2-4_1_3519.17216.621.1 1 2 1 8.69 5.77 3.83 2.93 72.8 5 0 0 4410.0 Hydrogen 5 (A) 9 AAAGAQLSW (A) 9 VGNAGLAAMA A A A G/A|Q\L\S\W 55.6 5 4 2 1 0 b-ion bias 59.463455 1.354002 874.439 874.442 -0.0027 -3.1 2.6999999999999997 5.57 2511.8 nuORFdb_human ENST00000596974.5_1_19:47249325-47251339:+ ENST00000596974.5_1_19:47249325-47251339:+ FKRP 5' uORF 5' uORF GN=FKRP 5' uORF E_20221201_NO30_400nL_HLAc1_2e7_directIP_titration_rep4_Slot2-4_1_3519 40mio-rep1 E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep1_Slot1-04_1_3496.17462.600.1 1 1 1 7.39 3.69 3.71 3.71 69.3 5 0 0 4920.0 Hydrogen 5 (A) 9 AAAGAQLSW (A) 9 AAGPGKTSW A A/A G A\Q\L\S\W 44.4 4 4 1 0 0 b-ion bias 60.318077 1.366494 874.4399 874.442 -0.0017 -2.0 1.2000000000000002 5.57 2511.8 nuORFdb_human ENST00000596974.5_1_19:47249325-47251339:+ ENST00000596974.5_1_19:47249325-47251339:+ FKRP 5' uORF 5' uORF GN=FKRP 5' uORF E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep1_Slot1-04_1_3496 40mio-rep2 E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep2_Slot2-5_1_3520.17150.616.1 1 1 1 8.12 4.09 3.0 4.03 70.1 6 0 0 3860.0 Hydrogen 5 (A) 9 AAAGAQLSW (A) 9 AAAAANLDSA A A/A G\A\Q\L\S\W 55.6 5 5 1 0 0 b-ion bias 59.241653 1.356796 874.4335 874.442 -0.0077 -8.9 -4.0 5.57 2511.8 nuORFdb_human ENST00000596974.5_1_19:47249325-47251339:+ ENST00000596974.5_1_19:47249325-47251339:+ FKRP 5' uORF 5' uORF GN=FKRP 5' uORF E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep2_Slot2-5_1_3520 40mio-rep3 E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep3_Slot2-5_1_3521.17181.621.1 1 1 1 8.78 3.31 5.98 5.46 71.3 6 0 0 4860.0 Hydrogen 5 (A) 9 AAAGAQLSW (A) 9 FGSLAGSVH A A/A G/A|Q\L\S\W 55.6 5 4 3 1 0 unbiased 59.350357 1.353935 874.4392 874.442 -0.0027 -3.1 1.4999999999999996 5.57 2511.8 nuORFdb_human ENST00000596974.5_1_19:47249325-47251339:+ ENST00000596974.5_1_19:47249325-47251339:+ FKRP 5' uORF 5' uORF GN=FKRP 5' uORF E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep3_Slot2-5_1_3521 Exploris_FAIMS VH20221207_MKP_FAIMS2022E_HLAc1_4e7_PDAC.22638.22638.2 2 1 1 14.69 12.07 4.24 2.62 93.1 8 1 1 1630000.0 Hydrogen 18 (D) 9 AAAGVRAAL (G) 9 AAAGLGARL A/A|A|G/V/R\A\A|L 100.0 9 5 6 3 0 dual b/y bias 0.999 100.0 47.05 120.0 -70.0 400.242 799.479 -0.0018 -2.3 0.30000000000000027 9.79 5328.1 nuORFdb_human ENST00000282382.8_2_5:114961385-114961538:- ENST00000282382.8_2_5:114961385-114961538:- TMED7-TICAM2 5' Overlap uORF 5' Overlap uORF GN=TMED7-TICAM2 5' Overlap uORF VH20221207_MKP_FAIMS2022E_HLAc1_4e7_PDAC 10mio-rep1 E_20221201_NO30_400nL_HLAc1_1e7_directIP_titration_rep1_Slot2-3_1_3512.11348.1598.2 2 1 1 13.68 10.11 1.0 3.57 90.6 8 1 1 3480.0 Hydrogen 3 (A) 9 AAALKAAAF (G) 9 AAKAALAAF A/A/A/L|K\A\A|A\F 100.0 9 5 5 2 0 unbiased 39.207188 0.75166 417.2437 833.488 -0.0072 -8.7 -0.9999999999999991 8.8 1248.5 nuORFdb_human ENST00000452825.7_2_X:53254339-53254378:- ENST00000452825.7_2_X:53254339-53254378:- KDM5C 5' uORF 5' uORF GN=KDM5C 5' uORF E_20221201_NO30_400nL_HLAc1_1e7_directIP_titration_rep1_Slot2-3_1_3512 10mio-rep2 E_20221201_NO30_400nL_HLAc1_1e7_directIP_titration_rep2_Slot2-3_1_3513.11293.1606.2 2 1 1 9.01 7.95 4.16 1.06 82.6 6 1 1 2290.0 Hydrogen 3 (A) 9 AAALKAAAF (G) 9 AAALILnF A A/A/L|K A\A|A\F 55.6 5 4 4 2 0 unbiased 39.015114 0.746548 417.2455 833.488 -0.0052 -6.3 0.40000000000000036 8.8 1248.5 nuORFdb_human ENST00000452825.7_2_X:53254339-53254378:- ENST00000452825.7_2_X:53254339-53254378:- KDM5C 5' uORF 5' uORF GN=KDM5C 5' uORF E_20221201_NO30_400nL_HLAc1_1e7_directIP_titration_rep2_Slot2-3_1_3513 20mio-rep1 E_20221201_NO30_400nL_HLAc1_2e7_directIP_titration_rep1_Slot2-4_1_3515.11247.1597.2 2 2 1 15.23 10.3 2.56 4.93 91.1 8 1 1 11500.0 Hydrogen 3 (A) 9 AAALKAAAF (G) 9 AAKAALAAF A/A/A/L|K|A\A|A\F 100.0 9 5 6 3 0 dual b/y bias 38.846687 0.752301 417.2454 833.488 -0.0052 -6.3 -0.09999999999999964 8.8 1248.5 nuORFdb_human ENST00000452825.7_2_X:53254339-53254378:- ENST00000452825.7_2_X:53254339-53254378:- KDM5C 5' uORF 5' uORF GN=KDM5C 5' uORF E_20221201_NO30_400nL_HLAc1_2e7_directIP_titration_rep1_Slot2-4_1_3515 20mio-rep2 E_20221201_NO30_400nL_HLAc1_2e7_directIP_titration_rep3_Slot2-4_1_3518.11379.1594.2 2 1 1 14.01 11.23 1.0 2.79 87.3 8 1 1 5980.0 Hydrogen 3 (A) 9 AAALKAAAF (G) 9 AAAKGLGVF A/A/A/L|K\A\A|A\F 100.0 9 5 5 2 0 unbiased 39.304237 0.753815 417.2446 833.488 -0.0052 -6.3 0.0 8.8 1248.5 nuORFdb_human ENST00000452825.7_2_X:53254339-53254378:- ENST00000452825.7_2_X:53254339-53254378:- KDM5C 5' uORF 5' uORF GN=KDM5C 5' uORF E_20221201_NO30_400nL_HLAc1_2e7_directIP_titration_rep3_Slot2-4_1_3518 20mio-rep3 E_20221201_NO30_400nL_HLAc1_2e7_directIP_titration_rep4_Slot2-4_1_3519.11286.1589.2 2 3 1 10.88 6.96 1.4 3.92 80.6 8 1 1 15100.0 Hydrogen 3 (A) 9 AAALKAAAF (G) 9 AAKAALAAF A/A/A/L|K\A\A|A\F 100.0 9 5 5 2 0 unbiased 38.98793 0.756978 417.2446 833.488 -0.0052 -6.3 -0.5 8.8 1248.5 nuORFdb_human ENST00000452825.7_2_X:53254339-53254378:- ENST00000452825.7_2_X:53254339-53254378:- KDM5C 5' uORF 5' uORF GN=KDM5C 5' uORF E_20221201_NO30_400nL_HLAc1_2e7_directIP_titration_rep4_Slot2-4_1_3519 40mio-rep1 E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep1_Slot1-04_1_3496.11539.1584.2 2 2 1 11.32 8.59 1.0 2.73 71.6 8 1 1 9340.0 Hydrogen 3 (A) 9 AAALKAAAF (G) 9 AAAKGLGVF A/A/A/L|K\A\A\A\F 100.0 9 5 4 1 0 unbiased 39.860085 0.760561 417.2465 833.488 -0.0032 -3.9 -0.6999999999999997 8.8 1248.5 nuORFdb_human ENST00000452825.7_2_X:53254339-53254378:- ENST00000452825.7_2_X:53254339-53254378:- KDM5C 5' uORF 5' uORF GN=KDM5C 5' uORF E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep1_Slot1-04_1_3496 40mio-rep4 E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep4_Slot2-5_1_3522.11263.1600.2 2 3 1 9.07 6.29 1.0 2.78 73.5 8 1 1 9970.0 Hydrogen 3 (A) 9 AAALKAAAF (G) 9 AAAKGLGVF A/A/A/L/K\A\A\A\F 100.0 9 4 4 0 0 unbiased 38.896488 0.750303 417.2452 833.488 -0.0052 -6.3 -1.5999999999999996 8.8 1248.5 nuORFdb_human ENST00000452825.7_2_X:53254339-53254378:- ENST00000452825.7_2_X:53254339-53254378:- KDM5C 5' uORF 5' uORF GN=KDM5C 5' uORF E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep4_Slot2-5_1_3522 40mio-rep1 E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep1_Slot1-04_1_3496.19382.600.1 1 1 1 12.31 2.96 7.67 9.35 85.9 8 0 0 5160.0 Hydrogen 2 (M) 10 AAAPAAGAIW (A) 10 NPGLQAIW A A/A/P\A|A\G\A\I\W 80.0 8 6 3 1 0 b-ion bias 66.955269 1.366006 898.478 898.478 -0.0001 -0.1 3.1 5.57 3073.4 smORF_v4|nuORFdb_human USP21_1:161129314-161129418:+ USP21_1:161129314-161129418:+|ENST00000492950.5_3_1:161129274-161129415:+ USP21 uORF 1:161129314-161129418:+ 1 USP21 5' uORF 5' uORF GN=USP21 E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep1_Slot1-04_1_3496 40mio-rep2 E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep2_Slot2-5_1_3520.19062.619.1 1 1 1 9.31 2.79 6.3 6.52 86.8 7 0 0 2560.0 Hydrogen 2 (M) 10 AAAPAAGAIW (A) 10 ATLHSAIW A A/A/P A\A\G\A\I\W 60.0 6 5 2 0 0 b-ion bias 65.850372 1.35472 898.4767 898.478 -0.0011 -1.3 3.6000000000000005 5.57 3073.4 smORF_v4|nuORFdb_human USP21_1:161129314-161129418:+ USP21_1:161129314-161129418:+|ENST00000492950.5_3_1:161129274-161129415:+ USP21 uORF 1:161129314-161129418:+ 1 USP21 5' uORF 5' uORF GN=USP21 E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep2_Slot2-5_1_3520 Exploris_FAIMS VH20221207_MKP_FAIMS2022E_HLAc1_4e7_PDAC.22155.22155.2 2 2 1 10.85 8.63 1.05 2.22 90.5 6 1 1 9680000.0 Hydrogen 29 (P) 9 AAAPRAAPL (P) 9 AAAPAARLP A/A|A|P/R A/A-P/L 77.8 7 2 6 2 1 y-ion bias 0.993 94.2 46.25 85.66 -70.0 419.2497 837.494 -0.0019 -2.3 0.30000000000000027 9.79 11822.5 nuORFdb_human ENST00000263642.2_1_2:163174829-163175141:- ENST00000263642.2_1_2:163174829-163175141:- IFIH1 5' uORF 5' uORF GN=IFIH1 5' uORF VH20221207_MKP_FAIMS2022E_HLAc1_4e7_PDAC SCP E_20221201_NO30_400nL_HLAc1_4e7_PDAC_Slot2-6_1_3524.11433.773.1 1 1 1 9.93 3.86 4.43 6.07 74.4 6 0 0 7500.0 Hydrogen 5 (A) 9 AAAPSVGAM (A) 9 AAGPSLQM A A/A/P S\V\G\A\M 55.6 5 4 2 0 0 b-ion bias 39.498554 1.262509 774.3777 774.381 -0.0035 -4.5 -2.1 5.57 15351.2 nuORFdb_human ENST00000490253.1_1_20:39766245-39788406:+ ENST00000490253.1_1_20:39766245-39788406:+ PLCG1 5' Overlap uORF 5' Overlap uORF GN=PLCG1 5' Overlap uORF E_20221201_NO30_400nL_HLAc1_4e7_PDAC_Slot2-6_1_3524 40mio-rep1 E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep1_Slot1-04_1_3496.8921.786.1 1 1 1 12.46 10.25 3.25 2.21 92.4 5 0 0 2190.0 Hydrogen 3 (A) 9 AAASAAPPV (-) 9 GATAAAPVP A A A S\A|A|P|P\V 55.6 5 5 3 3 0 dual b/y bias 30.820631 1.254418 754.4093 754.409 -0.0004 -0.5 2.7 5.57 956.1 nuORFdb_human ENST00000398645.6_1_11:66886757-66886790:+ ENST00000398645.6_1_11:66886757-66886790:+ KDM2A 5' uORF 5' uORF GN=KDM2A 5' uORF E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep1_Slot1-04_1_3496 40mio-rep1 E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep1_Slot1-04_1_3496.7195.1578.2 2 1 1 8.09 6.44 3.6 1.65 70.0 5 0 1 3500.0 Hydrogen 24 (A) 9 AAASPRPAL (A) 9 AAASAPPIR A/A/A/S|P/R P A L 55.6 5 1 5 1 0 y-ion bias 24.862057 0.764387 427.2459 853.489 -0.0043 -5.0 -1.7999999999999998 9.79 10607.2 nuORFdb_human ENST00000438277.5_1_2:128848793-128867211:+ ENST00000438277.5_1_2:128848793-128867211:+ UGGT1 Nonsense Mediated Decay Other GN=UGGT1 Nonsense Mediated Decay E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep1_Slot1-04_1_3496 40mio-rep2 E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep2_Slot2-5_1_3520.6941.1577.2 2 1 1 13.36 9.09 5.27 4.26 83.8 7 0 1 3450.0 Hydrogen 24 (A) 9 AAASPRPAL (A) 9 AAASAPPIR A/A/A/S|P/R\P A\L 77.8 7 3 5 1 0 unbiased 23.983805 0.765097 427.2448 853.489 -0.0063 -7.4 -2.5 9.79 10607.2 nuORFdb_human ENST00000438277.5_1_2:128848793-128867211:+ ENST00000438277.5_1_2:128848793-128867211:+ UGGT1 Nonsense Mediated Decay Other GN=UGGT1 Nonsense Mediated Decay E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep2_Slot2-5_1_3520 20mio-rep1 E_20221201_NO30_400nL_HLAc1_2e7_directIP_titration_rep1_Slot2-4_1_3515.12926.598.1 1 3 1 9.64 5.53 3.2 4.11 74.3 6 0 0 7770.0 Hydrogen 5 (A) 10 AAATAAAATW (G) 10 AAAAATAAWT A A A/T A|A\A\A\T\W 50.0 5 5 2 1 0 b-ion bias 44.646046 1.367653 904.4475 904.452 -0.0053 -5.9 0.2999999999999998 5.57 2630.9 smORF_v4|nuORFdb_human HS3ST3B1_17:14204635-14204724:+ HS3ST3B1_17:14204635-14204724:+|ENST00000466596.5_1_17:14204427-14204721:+ HS3ST3B1 uORF 17:14204635-14204724:+ 17 HS3ST3B1 5' uORF 5' uORF GN=HS3ST3B1 E_20221201_NO30_400nL_HLAc1_2e7_directIP_titration_rep1_Slot2-4_1_3515 40mio-rep1 E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep1_Slot1-04_1_3496.13200.577.1 1 2 1 14.2 7.25 4.23 6.96 88.0 6 0 0 10400.0 Hydrogen 5 (A) 10 AAATAAAATW (G) 10 TNLGAPGAFG A A A T|A|A\A\A\T\W 60.0 6 6 2 2 0 b-ion bias 45.59734 1.379808 904.4498 904.452 -0.0023 -2.6 0.6000000000000001 5.57 2630.9 smORF_v4|nuORFdb_human HS3ST3B1_17:14204635-14204724:+ HS3ST3B1_17:14204635-14204724:+|ENST00000466596.5_1_17:14204427-14204721:+ HS3ST3B1 uORF 17:14204635-14204724:+ 17 HS3ST3B1 5' uORF 5' uORF GN=HS3ST3B1 E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep1_Slot1-04_1_3496 40mio-rep2 E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep2_Slot2-5_1_3520.12899.590.1 1 1 1 13.68 9.08 4.75 4.61 88.8 7 0 0 4180.0 Hydrogen 5 (A) 10 AAATAAAATW (G) 10 AAAAATAAWT A A A/T\A\A|A\A|T\W 70.0 7 6 3 2 0 b-ion bias 44.559517 1.372527 904.4482 904.452 -0.0043 -4.8 0.10000000000000053 5.57 2630.9 smORF_v4|nuORFdb_human HS3ST3B1_17:14204635-14204724:+ HS3ST3B1_17:14204635-14204724:+|ENST00000466596.5_1_17:14204427-14204721:+ HS3ST3B1 uORF 17:14204635-14204724:+ 17 HS3ST3B1 5' uORF 5' uORF GN=HS3ST3B1 E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep2_Slot2-5_1_3520 40mio-rep3 E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep3_Slot2-5_1_3521.12913.592.1 1 2 1 7.63 3.03 1.05 4.59 71.2 6 0 0 6570.0 Hydrogen 5 (A) 10 AAATAAAATW (G) 10 AAAAATAAWT A A A T\A\A\A\A\T\W 60.0 6 6 0 0 0 b-ion bias 44.612206 1.371344 904.4482 904.452 -0.0043 -4.8 -0.20000000000000018 5.57 2630.9 smORF_v4|nuORFdb_human HS3ST3B1_17:14204635-14204724:+ HS3ST3B1_17:14204635-14204724:+|ENST00000466596.5_1_17:14204427-14204721:+ HS3ST3B1 uORF 17:14204635-14204724:+ 17 HS3ST3B1 5' uORF 5' uORF GN=HS3ST3B1 E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep3_Slot2-5_1_3521 40mio-rep4 E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep4_Slot2-5_1_3522.12903.589.1 1 2 1 12.85 5.78 7.94 7.07 89.2 7 0 0 8210.0 Hydrogen 5 (A) 10 AAATAAAATW (G) 10 TNLGAPGAFG A A/A/T A|A\A\A\T\W 60.0 6 5 3 1 0 unbiased 44.55814 1.372766 904.4511 904.452 -0.0013 -1.4 3.3000000000000003 5.57 2630.9 smORF_v4|nuORFdb_human HS3ST3B1_17:14204635-14204724:+ HS3ST3B1_17:14204635-14204724:+|ENST00000466596.5_1_17:14204427-14204721:+ HS3ST3B1 uORF 17:14204635-14204724:+ 17 HS3ST3B1 5' uORF 5' uORF GN=HS3ST3B1 E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep4_Slot2-5_1_3522 10mio-rep1 E_20221201_NO30_400nL_HLAc1_1e7_directIP_titration_rep1_Slot2-3_1_3512.14772.1541.2 2 1 1 10.43 5.6 3.77 4.83 78.1 7 1 1 3920.0 Hydrogen 113 (A) 9 AAGLRTVCF (R) 9 ALLGGTGVCF A/A/G/L/R T\V|C\F 77.8 7 3 5 1 0 unbiased Carbamidomethylation C:Carbamidomethylation, 51.032631 0.787852 497.7569 994.514 -0.0071 -7.2 0.5 9.79 13659.4 nuORFdb_human ENST00000292363.9_1_19:651790-663233:- ENST00000292363.9_1_19:651790-663233:- RNF126 3' Overlap dORF 3' Overlap dORF GN=RNF126 3' Overlap dORF E_20221201_NO30_400nL_HLAc1_1e7_directIP_titration_rep1_Slot2-3_1_3512 10mio-rep2 E_20221201_NO30_400nL_HLAc1_1e7_directIP_titration_rep2_Slot2-3_1_3513.14728.1535.2 2 1 1 9.05 3.73 3.27 5.32 73.3 6 1 1 3580.0 Hydrogen 113 (A) 9 AAGLRTVCF (R) 9 ALLGGTGVCF A/A/G L|R T/V|C\F 55.6 5 3 5 2 0 unbiased Carbamidomethylation C:Carbamidomethylation, 50.878166 0.791479 497.7574 994.514 -0.0071 -7.2 -0.5 9.79 13659.4 nuORFdb_human ENST00000292363.9_1_19:651790-663233:- ENST00000292363.9_1_19:651790-663233:- RNF126 3' Overlap dORF 3' Overlap dORF GN=RNF126 3' Overlap dORF E_20221201_NO30_400nL_HLAc1_1e7_directIP_titration_rep2_Slot2-3_1_3513 10mio-rep3 E_20221201_NO30_400nL_HLAc1_1e7_directIP_titration_rep3_Slot2-3_1_3514.14664.1542.2 2 3 1 11.25 4.76 3.93 6.49 73.0 7 1 1 9880.0 Hydrogen 113 (A) 9 AAGLRTVCF (R) 9 AKGELLGCF A/A/G L/R\T\V|C\F 77.8 7 4 4 1 0 unbiased Carbamidomethylation C:Carbamidomethylation, 50.659481 0.787245 497.757 994.514 -0.0071 -7.2 -0.9000000000000004 9.79 13659.4 nuORFdb_human ENST00000292363.9_1_19:651790-663233:- ENST00000292363.9_1_19:651790-663233:- RNF126 3' Overlap dORF 3' Overlap dORF GN=RNF126 3' Overlap dORF E_20221201_NO30_400nL_HLAc1_1e7_directIP_titration_rep3_Slot2-3_1_3514 20mio-rep1 E_20221201_NO30_400nL_HLAc1_2e7_directIP_titration_rep1_Slot2-4_1_3515.14641.1527.2 2 1 1 7.39 3.11 3.58 4.29 63.1 7 1 1 6610.0 Hydrogen 113 (A) 9 AAGLRTVCF (R) 9 AKGELLGCF A/A/G L/R/T\V|C\F 77.8 7 3 5 1 0 unbiased Carbamidomethylation C:Carbamidomethylation, 50.566814 0.796358 497.7565 994.514 -0.0091 -9.2 -2.999999999999999 9.79 13659.4 nuORFdb_human ENST00000292363.9_1_19:651790-663233:- ENST00000292363.9_1_19:651790-663233:- RNF126 3' Overlap dORF 3' Overlap dORF GN=RNF126 3' Overlap dORF E_20221201_NO30_400nL_HLAc1_2e7_directIP_titration_rep1_Slot2-4_1_3515 20mio-rep2 E_20221201_NO30_400nL_HLAc1_2e7_directIP_titration_rep3_Slot2-4_1_3518.14728.1510.2 2 2 1 10.72 4.36 2.88 6.36 71.6 7 1 1 8780.0 Hydrogen 113 (A) 9 AAGLRTVCF (R) 9 AAAAAATVMAF A/A/G/L/R T\V|C\F 77.8 7 3 5 1 0 unbiased Carbamidomethylation C:Carbamidomethylation, 50.871956 0.807159 497.7586 994.514 -0.0031 -3.2 3.0999999999999996 9.79 13659.4 nuORFdb_human ENST00000292363.9_1_19:651790-663233:- ENST00000292363.9_1_19:651790-663233:- RNF126 3' Overlap dORF 3' Overlap dORF GN=RNF126 3' Overlap dORF E_20221201_NO30_400nL_HLAc1_2e7_directIP_titration_rep3_Slot2-4_1_3518 20mio-rep3 E_20221201_NO30_400nL_HLAc1_2e7_directIP_titration_rep4_Slot2-4_1_3519.14667.1507.2 2 1 1 9.56 4.15 2.31 5.41 77.1 6 1 1 7080.0 Hydrogen 113 (A) 9 AAGLRTVCF (R) 9 VNVVGLSCF A/A/G L|R\T V|C\F 55.6 5 4 4 2 0 unbiased Carbamidomethylation C:Carbamidomethylation, 50.660629 0.809246 497.7587 994.514 -0.0031 -3.2 2.5999999999999996 9.79 13659.4 nuORFdb_human ENST00000292363.9_1_19:651790-663233:- ENST00000292363.9_1_19:651790-663233:- RNF126 3' Overlap dORF 3' Overlap dORF GN=RNF126 3' Overlap dORF E_20221201_NO30_400nL_HLAc1_2e7_directIP_titration_rep4_Slot2-4_1_3519 40mio-rep1 E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep1_Slot1-04_1_3496.14918.1503.2 2 3 1 13.57 5.24 6.69 8.33 88.2 7 1 1 44600.0 Hydrogen 113 (A) 9 AAGLRTVCF (R) 9 AASGSRLWF A/A/G L/R|T\V|C\F 77.8 7 4 5 2 0 unbiased Carbamidomethylation C:Carbamidomethylation, 51.531307 0.811837 497.7584 994.514 -0.0051 -5.2 -2.0 9.79 13659.4 nuORFdb_human ENST00000292363.9_1_19:651790-663233:- ENST00000292363.9_1_19:651790-663233:- RNF126 3' Overlap dORF 3' Overlap dORF GN=RNF126 3' Overlap dORF E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep1_Slot1-04_1_3496 40mio-rep2 E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep2_Slot2-5_1_3520.14610.1511.2 2 1 1 13.74 4.63 7.56 9.11 83.1 8 1 1 13600.0 Hydrogen 113 (A) 9 AAGLRTVCF (R) 9 VNVVGLSCF A/A/G/L/R|T\V|C\F 100.0 9 4 6 2 0 unbiased Carbamidomethylation C:Carbamidomethylation, 50.469151 0.80638 497.7574 994.514 -0.0071 -7.2 -2.3 9.79 13659.4 nuORFdb_human ENST00000292363.9_1_19:651790-663233:- ENST00000292363.9_1_19:651790-663233:- RNF126 3' Overlap dORF 3' Overlap dORF GN=RNF126 3' Overlap dORF E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep2_Slot2-5_1_3520 40mio-rep3 E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep3_Slot2-5_1_3521.14620.1516.2 2 4 1 16.75 7.58 7.85 9.17 91.3 8 1 1 36400.0 Hydrogen 113 (A) 9 AAGLRTVCF (R) 9 AAAAAATVMAF A/A/G/L/R|T|V|C\F 100.0 9 4 7 3 0 dual b/y bias Carbamidomethylation C:Carbamidomethylation, 50.50584 0.80363 497.7572 994.514 -0.0071 -7.2 -2.6000000000000005 9.79 13659.4 nuORFdb_human ENST00000292363.9_1_19:651790-663233:- ENST00000292363.9_1_19:651790-663233:- RNF126 3' Overlap dORF 3' Overlap dORF GN=RNF126 3' Overlap dORF E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep3_Slot2-5_1_3521 40mio-rep4 E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep4_Slot2-5_1_3522.14611.1517.2 2 2 1 13.47 5.45 7.35 8.02 82.3 8 1 1 16200.0 Hydrogen 113 (A) 9 AAGLRTVCF (R) 9 VNVVGLSCF A/A/G/L/R|T\V|C\F 100.0 9 4 6 2 0 unbiased Carbamidomethylation C:Carbamidomethylation, 50.460808 0.802798 497.7565 994.514 -0.0071 -7.2 -2.5 9.79 13659.4 nuORFdb_human ENST00000292363.9_1_19:651790-663233:- ENST00000292363.9_1_19:651790-663233:- RNF126 3' Overlap dORF 3' Overlap dORF GN=RNF126 3' Overlap dORF E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep4_Slot2-5_1_3522 10mio-rep1 E_20221201_NO30_400nL_HLAc1_1e7_directIP_titration_rep1_Slot2-3_1_3512.19933.649.1 1 1 1 7.71 4.91 3.35 2.8 73.0 6 0 0 1680.0 Hydrogen 9 (A) 9 AALPNAAAW (H) 9 LPGGAAAAAW A A/L/P N\A\A\A\W 55.6 5 4 2 0 0 b-ion bias 68.856033 1.336935 884.4548 884.462 -0.0075 -8.5 -0.7999999999999998 5.57 4773.4 nuORFdb_human ENST00000345392.2_1_3:10183791-10191525:+ ENST00000345392.2_1_3:10183791-10191525:+ VHL Out-of-Frame Out-of-Frame GN=VHL Out-of-Frame E_20221201_NO30_400nL_HLAc1_1e7_directIP_titration_rep1_Slot2-3_1_3512 10mio-rep2 E_20221201_NO30_400nL_HLAc1_1e7_directIP_titration_rep2_Slot2-3_1_3513.19867.638.1 1 1 1 9.33 6.94 3.39 2.39 77.4 6 0 0 1750.0 Hydrogen 9 (A) 9 AALPNAAAW (H) 9 LPGGAAAAAW A A/L/P N|A\A\A\W 55.6 5 4 3 1 0 unbiased 68.623283 1.343272 884.4574 884.462 -0.0055 -6.2 0.5 5.57 4773.4 nuORFdb_human ENST00000345392.2_1_3:10183791-10191525:+ ENST00000345392.2_1_3:10183791-10191525:+ VHL Out-of-Frame Out-of-Frame GN=VHL Out-of-Frame E_20221201_NO30_400nL_HLAc1_1e7_directIP_titration_rep2_Slot2-3_1_3513 20mio-rep1 E_20221201_NO30_400nL_HLAc1_2e7_directIP_titration_rep1_Slot2-4_1_3515.19810.643.1 1 1 1 8.98 5.73 3.31 3.25 80.7 6 0 0 2490.0 Hydrogen 9 (A) 9 AALPNAAAW (H) 9 LPGGAAAAAW A A/L/P N|A\A\A\W 55.6 5 4 3 1 0 unbiased 68.412704 1.340366 884.4584 884.462 -0.0045 -5.1 1.1000000000000005 5.57 4773.4 nuORFdb_human ENST00000345392.2_1_3:10183791-10191525:+ ENST00000345392.2_1_3:10183791-10191525:+ VHL Out-of-Frame Out-of-Frame GN=VHL Out-of-Frame E_20221201_NO30_400nL_HLAc1_2e7_directIP_titration_rep1_Slot2-4_1_3515 20mio-rep2 E_20221201_NO30_400nL_HLAc1_2e7_directIP_titration_rep3_Slot2-4_1_3518.19876.628.1 1 1 1 8.93 6.89 3.44 2.04 79.1 6 0 0 2420.0 Hydrogen 9 (A) 9 AALPNAAAW (H) 9 LPGGAAAAAW A A/L/P N\A\A\A\W 55.6 5 4 2 0 0 b-ion bias 68.656624 1.349363 884.4576 884.462 -0.0045 -5.1 1.2000000000000002 5.57 4773.4 nuORFdb_human ENST00000345392.2_1_3:10183791-10191525:+ ENST00000345392.2_1_3:10183791-10191525:+ VHL Out-of-Frame Out-of-Frame GN=VHL Out-of-Frame E_20221201_NO30_400nL_HLAc1_2e7_directIP_titration_rep3_Slot2-4_1_3518 20mio-rep3 E_20221201_NO30_400nL_HLAc1_2e7_directIP_titration_rep4_Slot2-4_1_3519.19873.633.1 1 2 1 8.82 6.62 3.58 2.2 81.2 6 0 0 6000.0 Hydrogen 9 (A) 9 AALPNAAAW (H) 9 LPGGAAAAAW A A/L/P N\A\A\A\W 55.6 5 4 2 0 0 b-ion bias 68.636581 1.346379 884.4606 884.462 -0.0015 -1.7 4.1 5.57 4773.4 nuORFdb_human ENST00000345392.2_1_3:10183791-10191525:+ ENST00000345392.2_1_3:10183791-10191525:+ VHL Out-of-Frame Out-of-Frame GN=VHL Out-of-Frame E_20221201_NO30_400nL_HLAc1_2e7_directIP_titration_rep4_Slot2-4_1_3519 40mio-rep1 E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep1_Slot1-04_1_3496.20029.611.1 1 2 1 9.98 7.27 3.21 2.71 84.5 6 0 0 8290.0 Hydrogen 9 (A) 9 AALPNAAAW (H) 9 LPGGAAAAAW A A/L/P N\A\A\A\W 55.6 5 4 2 0 0 b-ion bias 69.190727 1.359938 884.4588 884.462 -0.0035 -3.9 -0.6999999999999997 5.57 4773.4 nuORFdb_human ENST00000345392.2_1_3:10183791-10191525:+ ENST00000345392.2_1_3:10183791-10191525:+ VHL Out-of-Frame Out-of-Frame GN=VHL Out-of-Frame E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep1_Slot1-04_1_3496 40mio-rep2 E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep2_Slot2-5_1_3520.19746.629.1 1 2 1 9.11 6.64 1.63 2.47 82.5 6 0 0 8300.0 Hydrogen 9 (A) 9 AALPNAAAW (H) 9 QPGAAALAW A A/L/P N\A\A\A\W 55.6 5 4 2 0 0 b-ion bias 68.213188 1.349074 884.4587 884.462 -0.0035 -3.9 1.0000000000000004 5.57 4773.4 nuORFdb_human ENST00000345392.2_1_3:10183791-10191525:+ ENST00000345392.2_1_3:10183791-10191525:+ VHL Out-of-Frame Out-of-Frame GN=VHL Out-of-Frame E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep2_Slot2-5_1_3520 40mio-rep1 E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep1_Slot1-04_1_3496.27086.345.1 1 2 1 11.19 7.03 4.89 4.17 80.6 5 0 0 10100.0 Hydrogen 16 (A) 10 AALSEPLAVW (M) 10 LSSLSPPAVEG A A L S E|P\L\A\V\W 50.0 5 5 1 1 0 b-ion bias 93.574875 1.517999 1056.572 1056.572 -0.0024 -2.3 0.9000000000000004 4.0 4502.1 smORF_v4|nuORFdb_human HPS1_10:100205057-100202997:- HPS1_10:100205057-100202997:-|ENST00000361490.8_1_10:100202974-100205153:- HPS1 overlapping.uORF 10:100205057-100202997:- 10 HPS1 5' Overlap uORF 5' Overlap uORF GN=HPS1 E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep1_Slot1-04_1_3496 40mio-rep3 E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep3_Slot2-5_1_3521.26874.363.1 1 1 1 7.26 3.91 7.26 63.7 6 0 0 3630.0 Hydrogen 16 (A) 10 AALSEPLAVW (M) 10 A A/L S/E/P L\A\V\W 40.0 4 3 3 0 0 unbiased 92.830681 1.507404 1056.5687 1056.572 -0.0024 -2.3 2.3 4.0 4502.1 smORF_v4|nuORFdb_human HPS1_10:100205057-100202997:- HPS1_10:100205057-100202997:-|ENST00000361490.8_1_10:100202974-100205153:- HPS1 overlapping.uORF 10:100205057-100202997:- 10 HPS1 5' Overlap uORF 5' Overlap uORF GN=HPS1 E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep3_Slot2-5_1_3521 40mio-rep2 E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep2_Slot2-5_1_3520.15318.1517.2 2 1 1 10.27 8.22 2.65 2.05 90.7 5 1 1 12100.0 Hydrogen 60 (R) 8 AARDPVLW (L) 8 VPDRAALW A/A R D|P\V|L\W 62.5 5 4 3 2 0 unbiased 52.914463 0.80306 464.2537 927.505 -0.004 -4.3 0.6000000000000005 5.88 8441.5 nuORFdb_human ENST00000429204.5_1_1:226925125-226925356:- ENST00000429204.5_1_1:226925125-226925356:- ITPKB 5' Overlap uORF 5' Overlap uORF GN=ITPKB 5' Overlap uORF E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep2_Slot2-5_1_3520 40mio-rep4 E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep4_Slot2-5_1_3522.15317.1513.2 2 1 1 11.54 7.99 4.09 3.56 91.3 6 1 1 9860.0 Hydrogen 60 (R) 8 AARDPVLW (L) 8 QERVPLW A/A R/D|P\V|L\W 75.0 6 4 4 2 0 unbiased 52.897789 0.805133 464.2541 927.505 -0.004 -4.3 0.40000000000000036 5.88 8441.5 nuORFdb_human ENST00000429204.5_1_1:226925125-226925356:- ENST00000429204.5_1_1:226925125-226925356:- ITPKB 5' Overlap uORF 5' Overlap uORF GN=ITPKB 5' Overlap uORF E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep4_Slot2-5_1_3522 Exploris_FAIMS VH20221207_MKP_FAIMS2022E_HLAc1_4e7_PDAC.57393.57393.2 2 2 1 7.97 5.46 0.08 2.51 72.1 6 0 0 25000000.0 Acetyl 2 (M) 9 AASISVPLS (M) 9 LKnDISPV A A\S\I\S\V P-L\S 55.6 5 5 0 0 1 b-ion bias 0.994 74.0 99.95 99.57 -70.0 443.7464 886.488 -0.0025 -2.8 -0.19999999999999973 5.57 2126.6 nuORFdb_human ENST00000411526.5_1_7:154720239-154720296:+ ENST00000411526.5_1_7:154720239-154720296:+ PAXIP1-AS2 Antisense lncRNA GN=PAXIP1-AS2 Antisense VH20221207_MKP_FAIMS2022E_HLAc1_4e7_PDAC 40mio-rep1 E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep1_Slot1-04_1_3496.4046.1730.3 3 1 1 8.53 5.78 1.78 2.75 89.5 6 1 2 3120.0 Hydrogen 4 (P) 8 AASKPRLY (H) 8 RPLPPPSAA A/A/S/K/P/R L-Y 75.0 6 0 5 0 1 y-ion bias 13.987168 0.667495 302.5096 905.52 -0.0049 -5.4 -2.2 9.99 3621.1 nuORFdb_human ENST00000340434.4_1_9:72349131-72356758:- ENST00000340434.4_1_9:72349131-72356758:- PTAR1 Out-of-Frame Out-of-Frame GN=PTAR1 Out-of-Frame E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep1_Slot1-04_1_3496 40mio-rep3 E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep3_Slot2-5_1_3521.3848.1754.3 3 1 1 7.45 3.5 3.09 3.95 88.7 7 1 2 1820.0 Hydrogen 4 (P) 8 AASKPRLY (H) 8 YAALAVRAA A/A/S/K/P/R\L-Y 100.0 8 1 5 0 1 y-ion bias 13.300427 0.651961 302.5086 905.52 -0.0079 -8.7 -4.1 9.99 3621.1 nuORFdb_human ENST00000340434.4_1_9:72349131-72356758:- ENST00000340434.4_1_9:72349131-72356758:- PTAR1 Out-of-Frame Out-of-Frame GN=PTAR1 Out-of-Frame E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep3_Slot2-5_1_3521 SCP E_20221201_NO30_400nL_HLAc1_4e7_PDAC_Slot2-6_1_3524.24025.430.1 1 2 1 7.45 1.36 4.68 6.1 68.0 6 0 0 8720.0 Hydrogen 69 (F) 9 AASPFPLCY (S) 9 GPYFSAMVPG A A/S/P/F/P L|C\Y 55.6 5 2 5 1 0 y-ion bias Carbamidomethylation C:Carbamidomethylation, 82.99968 1.46765 1025.4723 1025.476 -0.0061 -5.9 -3.5000000000000004 5.57 9424.3 smORF_v4|nuORFdb_human ZNF672_1:249138823-249141395:+ ZNF672_1:249138823-249141395:+|ENST00000510904.5_1_1:249138801-249141389:+ ZNF672 uORF 1:249138823-249141395:+ 1 ZNF672 5' uORF 5' uORF GN=ZNF672 E_20221201_NO30_400nL_HLAc1_4e7_PDAC_Slot2-6_1_3524 10mio-rep1 E_20221201_NO30_400nL_HLAc1_1e7_directIP_titration_rep1_Slot2-3_1_3512.13328.751.1 1 1 1 9.01 6.31 2.26 2.7 83.2 6 0 0 3290.0 Hydrogen 27 (G) 9 AASPVAISA (Q) 9 AASLAPVSA A A/S/P V\A\I\S\A 55.6 5 4 2 0 0 b-ion bias 46.045086 1.275559 786.4325 786.436 -0.0026 -3.3 4.4 5.57 5797.5 smORF_v4|nuORFdb_human ATF5_19:50432653-50434163:+ ATF5_19:50432653-50434163:+|ENST00000423777.6_1_19:50432412-50434160:+|ENST00000597227.5_1_19:50432514-50434160:+|ENST00000596658.1_1_19:50433558-50434160:+ ATF5 overlapping.uORF 19:50432653-50434163:+ 19 ATF5 5' Overlap uORF 5' Overlap uORF GN=ATF5 E_20221201_NO30_400nL_HLAc1_1e7_directIP_titration_rep1_Slot2-3_1_3512 10mio-rep2 E_20221201_NO30_400nL_HLAc1_1e7_directIP_titration_rep2_Slot2-3_1_3513.13286.752.1 1 1 1 9.51 6.98 1.7 2.53 76.8 6 0 0 2680.0 Hydrogen 27 (G) 9 AASPVAISA (Q) 9 AASLAPVSA A A/S/P V\A\I\S\A 55.6 5 4 2 0 0 b-ion bias 45.899284 1.275043 786.4285 786.436 -0.0066 -8.4 -1.7000000000000002 5.57 5797.5 smORF_v4|nuORFdb_human ATF5_19:50432653-50434163:+ ATF5_19:50432653-50434163:+|ENST00000423777.6_1_19:50432412-50434160:+|ENST00000597227.5_1_19:50432514-50434160:+|ENST00000596658.1_1_19:50433558-50434160:+ ATF5 overlapping.uORF 19:50432653-50434163:+ 19 ATF5 5' Overlap uORF 5' Overlap uORF GN=ATF5 E_20221201_NO30_400nL_HLAc1_1e7_directIP_titration_rep2_Slot2-3_1_3513 10mio-rep3 E_20221201_NO30_400nL_HLAc1_1e7_directIP_titration_rep3_Slot2-3_1_3514.13243.751.1 1 1 1 11.14 7.19 3.83 3.94 86.7 6 0 0 2600.0 Hydrogen 27 (G) 9 AASPVAISA (Q) 9 ASAPGIISA A A/S/P V\A\I\S\A 55.6 5 4 2 0 0 b-ion bias 45.752579 1.275243 786.4309 786.436 -0.0046 -5.8 0.5 5.57 5797.5 smORF_v4|nuORFdb_human ATF5_19:50432653-50434163:+ ATF5_19:50432653-50434163:+|ENST00000423777.6_1_19:50432412-50434160:+|ENST00000597227.5_1_19:50432514-50434160:+|ENST00000596658.1_1_19:50433558-50434160:+ ATF5 overlapping.uORF 19:50432653-50434163:+ 19 ATF5 5' Overlap uORF 5' Overlap uORF GN=ATF5 E_20221201_NO30_400nL_HLAc1_1e7_directIP_titration_rep3_Slot2-3_1_3514 20mio-rep1 E_20221201_NO30_400nL_HLAc1_2e7_directIP_titration_rep1_Slot2-4_1_3515.13212.751.1 1 1 1 11.84 9.2 3.28 2.63 85.6 7 0 0 3650.0 Hydrogen 27 (G) 9 AASPVAISA (Q) 9 ASAPGIISA A A/S/P\V\A\I\S\A 77.8 7 5 2 0 0 b-ion bias 45.63372 1.275728 786.4318 786.436 -0.0036 -4.6 1.6000000000000005 5.57 5797.5 smORF_v4|nuORFdb_human ATF5_19:50432653-50434163:+ ATF5_19:50432653-50434163:+|ENST00000423777.6_1_19:50432412-50434160:+|ENST00000597227.5_1_19:50432514-50434160:+|ENST00000596658.1_1_19:50433558-50434160:+ ATF5 overlapping.uORF 19:50432653-50434163:+ 19 ATF5 5' Overlap uORF 5' Overlap uORF GN=ATF5 E_20221201_NO30_400nL_HLAc1_2e7_directIP_titration_rep1_Slot2-4_1_3515 20mio-rep2 E_20221201_NO30_400nL_HLAc1_2e7_directIP_titration_rep3_Slot2-4_1_3518.13315.742.1 1 1 1 10.17 8.56 2.16 1.6 83.8 5 0 0 4800.0 Hydrogen 27 (G) 9 AASPVAISA (Q) 9 ASVPAALAS A A S/P V\A\I\S\A 44.4 4 4 1 0 0 b-ion bias 45.990421 1.280699 786.433 786.436 -0.0026 -3.3 3.0 5.57 5797.5 smORF_v4|nuORFdb_human ATF5_19:50432653-50434163:+ ATF5_19:50432653-50434163:+|ENST00000423777.6_1_19:50432412-50434160:+|ENST00000597227.5_1_19:50432514-50434160:+|ENST00000596658.1_1_19:50433558-50434160:+ ATF5 overlapping.uORF 19:50432653-50434163:+ 19 ATF5 5' Overlap uORF 5' Overlap uORF GN=ATF5 E_20221201_NO30_400nL_HLAc1_2e7_directIP_titration_rep3_Slot2-4_1_3518 20mio-rep3 E_20221201_NO30_400nL_HLAc1_2e7_directIP_titration_rep4_Slot2-4_1_3519.13245.740.1 1 1 1 10.65 9.0 2.53 1.65 87.5 6 0 0 3240.0 Hydrogen 27 (G) 9 AASPVAISA (Q) 9 ASAPGIISA A A/S/P V\A\I\S\A 55.6 5 4 2 0 0 b-ion bias 45.752079 1.281941 786.4293 786.436 -0.0066 -8.4 -2.6000000000000005 5.57 5797.5 smORF_v4|nuORFdb_human ATF5_19:50432653-50434163:+ ATF5_19:50432653-50434163:+|ENST00000423777.6_1_19:50432412-50434160:+|ENST00000597227.5_1_19:50432514-50434160:+|ENST00000596658.1_1_19:50433558-50434160:+ ATF5 overlapping.uORF 19:50432653-50434163:+ 19 ATF5 5' Overlap uORF 5' Overlap uORF GN=ATF5 E_20221201_NO30_400nL_HLAc1_2e7_directIP_titration_rep4_Slot2-4_1_3519 40mio-rep1 E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep1_Slot1-04_1_3496.13503.721.1 1 1 1 12.61 10.13 4.01 2.48 90.3 6 0 0 5420.0 Hydrogen 27 (G) 9 AASPVAISA (Q) 9 ASAPGIISA A A/S/P V\A\I\S\A 55.6 5 4 2 0 0 b-ion bias 46.643925 1.293794 786.4331 786.436 -0.0026 -3.3 -0.09999999999999964 5.57 5797.5 smORF_v4|nuORFdb_human ATF5_19:50432653-50434163:+ ATF5_19:50432653-50434163:+|ENST00000423777.6_1_19:50432412-50434160:+|ENST00000597227.5_1_19:50432514-50434160:+|ENST00000596658.1_1_19:50433558-50434160:+ ATF5 overlapping.uORF 19:50432653-50434163:+ 19 ATF5 5' Overlap uORF 5' Overlap uORF GN=ATF5 E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep1_Slot1-04_1_3496 40mio-rep2 E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep2_Slot2-5_1_3520.13211.741.1 1 1 1 12.1 9.6 2.5 2.5 88.7 6 0 0 4820.0 Hydrogen 27 (G) 9 AASPVAISA (Q) 9 AASLAPVSA A A/S/P V\A\I\S\A 55.6 5 4 2 0 0 b-ion bias 45.637234 1.281276 786.4326 786.436 -0.0026 -3.3 1.6000000000000005 5.57 5797.5 smORF_v4|nuORFdb_human ATF5_19:50432653-50434163:+ ATF5_19:50432653-50434163:+|ENST00000423777.6_1_19:50432412-50434160:+|ENST00000597227.5_1_19:50432514-50434160:+|ENST00000596658.1_1_19:50433558-50434160:+ ATF5 overlapping.uORF 19:50432653-50434163:+ 19 ATF5 5' Overlap uORF 5' Overlap uORF GN=ATF5 E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep2_Slot2-5_1_3520 40mio-rep4 E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep4_Slot2-5_1_3522.13196.733.1 1 1 1 10.49 8.36 1.84 2.14 86.5 6 0 0 3310.0 Hydrogen 27 (G) 9 AASPVAISA (Q) 9 ASAPGIISA A A/S/P V\A\I\S\A 55.6 5 4 2 0 0 b-ion bias 45.570889 1.286112 786.4299 786.436 -0.0056 -7.1 -2.3999999999999995 5.57 5797.5 smORF_v4|nuORFdb_human ATF5_19:50432653-50434163:+ ATF5_19:50432653-50434163:+|ENST00000423777.6_1_19:50432412-50434160:+|ENST00000597227.5_1_19:50432514-50434160:+|ENST00000596658.1_1_19:50433558-50434160:+ ATF5 overlapping.uORF 19:50432653-50434163:+ 19 ATF5 5' Overlap uORF 5' Overlap uORF GN=ATF5 E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep4_Slot2-5_1_3522 5mio-rep1 E_20221201_NO30_400nL_HLAc1_5e6_directIP_titration_rep1_Slot1-37_1_3502.13575.738.1 1 2 1 9.01 5.62 3.43 3.39 74.1 6 0 0 2700.0 Hydrogen 27 (G) 9 AASPVAISA (Q) 9 AASLAPVSA A A/S/P V|A\I\S\A 55.6 5 4 3 1 0 unbiased 46.890869 1.283109 786.4268 786.436 -0.0086 -10.9 -3.9000000000000004 5.57 5797.5 smORF_v4|nuORFdb_human ATF5_19:50432653-50434163:+ ATF5_19:50432653-50434163:+|ENST00000423777.6_1_19:50432412-50434160:+|ENST00000597227.5_1_19:50432514-50434160:+|ENST00000596658.1_1_19:50433558-50434160:+ ATF5 overlapping.uORF 19:50432653-50434163:+ 19 ATF5 5' Overlap uORF 5' Overlap uORF GN=ATF5 E_20221201_NO30_400nL_HLAc1_5e6_directIP_titration_rep1_Slot1-37_1_3502 40mio-rep4 E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep4_Slot2-5_1_3522.5485.1611.2 2 1 1 10.19 7.77 2.08 2.41 84.0 7 1 1 6640.0 Hydrogen 9 (M) 8 AASVRGSF (G) 8 DPPRAPAA A/A/S/V/R\G\S\F 100.0 8 3 4 0 0 unbiased 18.949711 0.743494 397.7085 794.415 -0.0048 -6.0 -1.2999999999999998 9.79 1809.0 nuORFdb_human ENST00000381070.7_1_5:64064760-64064817:+ ENST00000381070.7_1_5:64064760-64064817:+ CWC27 5' uORF 5' uORF GN=CWC27 5' uORF E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep4_Slot2-5_1_3522 20mio-rep2 E_20221201_NO30_400nL_HLAc1_2e7_directIP_titration_rep3_Slot2-4_1_3518.11441.1487.2 2 1 1 11.54 3.61 8.95 7.93 73.6 7 1 1 6240.0 Hydrogen 43 (E) 10 AEAAGSRVVW (R) 10 AEAGVKREDA A/E/A|A|G S/R V|V\W 60.0 6 4 6 3 0 unbiased 39.518242 0.821438 523.2695 1045.543 -0.0098 -9.4 -3.1000000000000005 6.05 8866.9 nuORFdb_human ENST00000614443.1_1_17:34891215-34892969:+ ENST00000614443.1_1_17:34891215-34892969:+ PIGW 5' Overlap uORF 5' Overlap uORF GN=PIGW 5' Overlap uORF E_20221201_NO30_400nL_HLAc1_2e7_directIP_titration_rep3_Slot2-4_1_3518 40mio-rep1 E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep1_Slot1-04_1_3496.11576.1453.2 2 1 1 11.49 4.56 7.05 6.92 77.9 7 1 1 10100.0 Hydrogen 43 (E) 10 AEAAGSRVVW (R) 10 AEAAKDERR A/E/A|A|G/S R V|V\W 70.0 7 4 6 3 0 unbiased 39.988403 0.842969 523.2722 1045.543 -0.0058 -5.5 -2.3 6.05 8866.9 nuORFdb_human ENST00000614443.1_1_17:34891215-34892969:+ ENST00000614443.1_1_17:34891215-34892969:+ PIGW 5' Overlap uORF 5' Overlap uORF GN=PIGW 5' Overlap uORF E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep1_Slot1-04_1_3496 40mio-rep3 E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep3_Slot2-5_1_3521.11279.1478.2 2 1 1 9.75 4.05 6.64 5.71 65.4 7 1 1 11900.0 Hydrogen 43 (E) 10 AEAAGSRVVW (R) 10 AEAALQRETG A/E/A|A|G/S R V\V\W 70.0 7 4 5 2 0 unbiased 38.965919 0.827374 523.2729 1045.543 -0.0038 -3.6 0.9999999999999996 6.05 8866.9 nuORFdb_human ENST00000614443.1_1_17:34891215-34892969:+ ENST00000614443.1_1_17:34891215-34892969:+ PIGW 5' Overlap uORF 5' Overlap uORF GN=PIGW 5' Overlap uORF E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep3_Slot2-5_1_3521 40mio-rep4 E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep4_Slot2-5_1_3522.11282.1476.2 2 1 1 11.86 2.45 10.29 9.41 71.3 8 1 1 11500.0 Hydrogen 43 (E) 10 AEAAGSRVVW (R) 10 EAAAEDKRR A E/A|A|G/S/R\V|V\W 80.0 8 5 6 3 0 unbiased 38.962063 0.828361 523.2716 1045.543 -0.0058 -5.5 -0.7999999999999998 6.05 8866.9 nuORFdb_human ENST00000614443.1_1_17:34891215-34892969:+ ENST00000614443.1_1_17:34891215-34892969:+ PIGW 5' Overlap uORF 5' Overlap uORF GN=PIGW 5' Overlap uORF E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep4_Slot2-5_1_3522 40mio-rep1 E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep1_Slot1-04_1_3496.20109.531.1 1 1 1 10.68 7.41 4.15 3.28 79.1 7 0 0 5460.0 Hydrogen 30 (K) 9 AEAGVLENL (A) 9 AQEVLENL A E/A/G\V|L|E\N\L 77.8 7 5 4 2 0 unbiased 69.466988 1.407549 915.4785 915.478 -0.0002 -0.2 3.0 3.8 13836.3 nuORFdb_human ENST00000539387.5_1_4:140216240-140223649:- ENST00000539387.5_1_4:140216240-140223649:- NDUFC1 5' Overlap uORF 5' Overlap uORF GN=NDUFC1 5' Overlap uORF E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep1_Slot1-04_1_3496 SCP E_20221201_NO30_400nL_HLAc1_4e7_PDAC_Slot2-6_1_3524.7870.516.1 1 1 1 8.11 5.84 2.05 2.27 67.6 5 0 0 4470.0 Hydrogen 188 (A) 10 AEAHAVGEAL (H) 10 EAHATTPGLA A E/A/H|A-V\G E A\L 50.0 5 3 3 1 1 unbiased 27.190472 1.41648 967.4795 967.484 -0.0053 -5.5 -3.1 4.51 28938.9 nuORFdb_human ENST00000606788.5_1_20:44518901-44519711:+ ENST00000606788.5_1_20:44518901-44519711:+ CTSA 5' uORF 5' uORF GN=CTSA 5' uORF E_20221201_NO30_400nL_HLAc1_4e7_PDAC_Slot2-6_1_3524 40mio-rep1 E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep1_Slot1-04_1_3496.7954.1535.2 2 1 1 9.89 5.88 4.28 4.01 77.1 6 1 1 8030.0 Hydrogen 61 (L) 9 AEAPSAPKM (S) 9 EAATGPLGW A E/A|P/S A|P/K-M 55.6 5 2 5 2 1 y-ion bias 27.482042 0.791355 451.2227 901.445 -0.0061 -6.7 -3.5 6.05 9649.9 nuORFdb_human ENST00000556667.1_1_14:37641590-37641863:+ ENST00000556667.1_1_14:37641590-37641863:+ SLC25A21-AS1 Antisense lncRNA GN=SLC25A21-AS1 Antisense E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep1_Slot1-04_1_3496 20mio-rep2 E_20221201_NO30_400nL_HLAc1_2e7_directIP_titration_rep3_Slot2-4_1_3518.3773.1542.2 2 2 1 12.44 7.63 4.81 4.81 85.0 8 1 1 5110.0 M69m Hydrogen 61 (L) 9 AEAPSAPKm (S) 9 AEAASPLCV A/E/A|P|S/A|P-K\m 100.0 9 4 6 3 1 dual b/y bias m:Oxidized methionine 13.044588 0.786986 459.2203 901.445 15.9879 -7.6 -1.2999999999999998 6.05 9649.9 nuORFdb_human ENST00000556667.1_1_14:37641590-37641863:+ ENST00000556667.1_1_14:37641590-37641863:+ SLC25A21-AS1 Antisense lncRNA GN=SLC25A21-AS1 Antisense E_20221201_NO30_400nL_HLAc1_2e7_directIP_titration_rep3_Slot2-4_1_3518 20mio-rep3 E_20221201_NO30_400nL_HLAc1_2e7_directIP_titration_rep4_Slot2-4_1_3519.3791.1554.2 2 2 1 11.44 7.63 4.28 3.81 84.9 6 1 1 5990.0 M69m Hydrogen 61 (L) 9 AEAPSAPKm (S) 9 AEAASPLCV A/E/A|P|S/A|P K m 66.7 6 3 6 3 0 dual b/y bias m:Oxidized methionine 13.104083 0.779184 459.2195 901.445 15.9879 -7.6 -1.7999999999999998 6.05 9649.9 nuORFdb_human ENST00000556667.1_1_14:37641590-37641863:+ ENST00000556667.1_1_14:37641590-37641863:+ SLC25A21-AS1 Antisense lncRNA GN=SLC25A21-AS1 Antisense E_20221201_NO30_400nL_HLAc1_2e7_directIP_titration_rep4_Slot2-4_1_3519 40mio-rep2 E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep2_Slot2-5_1_3520.3793.1536.2 2 3 1 13.72 9.45 4.24 4.27 91.7 7 1 1 14900.0 M69m Hydrogen 61 (L) 9 AEAPSAPKm (S) 9 AEAASPLCV A/E/A|P|S/A|P K\m 77.8 7 4 6 3 0 dual b/y bias m:Oxidized methionine 13.112644 0.790871 459.2203 901.445 15.9879 -7.6 -2.6999999999999993 6.05 9649.9 nuORFdb_human ENST00000556667.1_1_14:37641590-37641863:+ ENST00000556667.1_1_14:37641590-37641863:+ SLC25A21-AS1 Antisense lncRNA GN=SLC25A21-AS1 Antisense E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep2_Slot2-5_1_3520 40mio-rep3 E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep3_Slot2-5_1_3521.3806.1565.2 2 4 1 10.54 7.36 3.18 3.18 86.8 6 1 1 19100.0 M69m Hydrogen 61 (L) 9 AEAPSAPKm (S) 9 AEAASPLCV A E/A|P/S A|P\K\m 55.6 5 4 4 2 0 unbiased m:Oxidized methionine 13.155466 0.7724 459.2208 901.445 15.9899 -5.4 -0.8000000000000007 6.05 9649.9 nuORFdb_human ENST00000556667.1_1_14:37641590-37641863:+ ENST00000556667.1_1_14:37641590-37641863:+ SLC25A21-AS1 Antisense lncRNA GN=SLC25A21-AS1 Antisense E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep3_Slot2-5_1_3521 40mio-rep4 E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep4_Slot2-5_1_3522.3794.1555.2 2 4 1 12.19 9.49 3.24 2.7 90.1 6 1 1 22500.0 M69m Hydrogen 61 (L) 9 AEAPSAPKm (S) 9 AEAASPLCV A/E/A|P/S/A|P K m 66.7 6 2 6 2 0 y-ion bias m:Oxidized methionine 13.108636 0.778619 459.2199 901.445 15.9879 -7.6 -2.8999999999999995 6.05 9649.9 nuORFdb_human ENST00000556667.1_1_14:37641590-37641863:+ ENST00000556667.1_1_14:37641590-37641863:+ SLC25A21-AS1 Antisense lncRNA GN=SLC25A21-AS1 Antisense E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep4_Slot2-5_1_3522 20mio-rep3 E_20221201_NO30_400nL_HLAc1_2e7_directIP_titration_rep4_Slot2-4_1_3519.13869.1407.2 2 1 1 8.55 5.88 4.14 2.66 58.7 5 1 1 6290.0 Hydrogen 3 (E) 10 AEARENFGAW (M) 10 MRGQRmGAW A E/A R E N|F\G|A\W 40.0 4 4 3 2 0 unbiased 47.9058 0.872271 575.7639 1150.528 -0.0069 -6.0 -0.20000000000000018 4.53 4976.7 smORF_v4|nuORFdb_human ARHGEF11_1:157015019-157015144:- ARHGEF11_1:157015019-157015144:-|ENST00000368194.7_1_1:157015019-157015145:- ARHGEF11 uORF 1:157015019-157015144:- 1 ARHGEF11 5' uORF 5' uORF GN=ARHGEF11 E_20221201_NO30_400nL_HLAc1_2e7_directIP_titration_rep4_Slot2-4_1_3519 40mio-rep1 E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep1_Slot1-04_1_3496.14168.1357.2 2 1 1 10.94 8.22 4.84 2.72 68.7 6 1 1 11900.0 Hydrogen 3 (E) 10 AEARENFGAW (M) 10 TWRSGSTGAW A E/A R\E N\F|G|A\W 40.0 4 5 3 2 0 unbiased 48.94067 0.903496 575.7669 1150.528 -0.0009 -0.8 2.4000000000000004 4.53 4976.7 smORF_v4|nuORFdb_human ARHGEF11_1:157015019-157015144:- ARHGEF11_1:157015019-157015144:-|ENST00000368194.7_1_1:157015019-157015145:- ARHGEF11 uORF 1:157015019-157015144:- 1 ARHGEF11 5' uORF 5' uORF GN=ARHGEF11 E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep1_Slot1-04_1_3496 40mio-rep3 E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep3_Slot2-5_1_3521.13822.1398.2 2 1 1 9.21 5.3 4.82 3.91 62.9 7 1 1 8440.0 Hydrogen 3 (E) 10 AEARENFGAW (M) 10 AEPTPGHNAAW A/E/A R\E N|F\G|A\W 60.0 6 5 4 2 0 unbiased 47.750996 0.877682 575.7646 1150.528 -0.0049 -4.2 0.39999999999999947 4.53 4976.7 smORF_v4|nuORFdb_human ARHGEF11_1:157015019-157015144:- ARHGEF11_1:157015019-157015144:-|ENST00000368194.7_1_1:157015019-157015145:- ARHGEF11 uORF 1:157015019-157015144:- 1 ARHGEF11 5' uORF 5' uORF GN=ARHGEF11 E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep3_Slot2-5_1_3521 40mio-rep4 E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep4_Slot2-5_1_3522.13822.1392.2 2 1 1 9.08 6.02 2.74 3.05 68.6 5 1 1 11300.0 Hydrogen 3 (E) 10 AEARENFGAW (M) 10 VmnSRVNGAW A E/A R E N|F|G\A\W 40.0 4 4 3 2 0 unbiased 47.734364 0.881062 575.7659 1150.528 -0.0029 -2.5 2.2 4.53 4976.7 smORF_v4|nuORFdb_human ARHGEF11_1:157015019-157015144:- ARHGEF11_1:157015019-157015144:-|ENST00000368194.7_1_1:157015019-157015145:- ARHGEF11 uORF 1:157015019-157015144:- 1 ARHGEF11 5' uORF 5' uORF GN=ARHGEF11 E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep4_Slot2-5_1_3522 10mio-rep2 E_20221201_NO30_400nL_HLAc1_1e7_directIP_titration_rep2_Slot2-3_1_3513.16401.424.1 1 2 1 12.21 3.39 9.14 8.82 88.3 8 0 0 3520.0 Hydrogen 10 (A) 11 AEASPGGAVAW (R) 11 SMPPMPVASV A E/A/S/P\G G\A\V\A\W 63.6 7 5 3 0 0 unbiased 56.655945 1.470874 1015.4762 1015.484 -0.0043 -4.3 2.4000000000000004 4.0 6797.8 smORF_v4|nuORFdb_human PSMD5-AS1_9:123605559-123607307:+ PSMD5-AS1_9:123605559-123607307:+|ENST00000640066.1_1_9:123605558-123607345:+|ENST00000640391.1_1_9:123605558-123609583:+ PSMD5-AS1 lncRNA 9:123605559-123607307:+ 9 PSMD5-AS1 Pseudogene Pseudogene GN=PSMD5-AS1 E_20221201_NO30_400nL_HLAc1_1e7_directIP_titration_rep2_Slot2-3_1_3513 10mio-rep3 E_20221201_NO30_400nL_HLAc1_1e7_directIP_titration_rep3_Slot2-3_1_3514.16356.428.1 1 2 1 16.87 5.32 12.85 11.55 94.5 9 0 0 4190.0 Hydrogen 10 (A) 11 AEASPGGAVAW (R) 11 EAGGKEPAADA A E/A/S/P\G|G|A\V\A\W 81.8 9 6 5 2 0 unbiased 56.500744 1.469019 1015.4777 1015.484 -0.0043 -4.3 2.0 4.0 6797.8 smORF_v4|nuORFdb_human PSMD5-AS1_9:123605559-123607307:+ PSMD5-AS1_9:123605559-123607307:+|ENST00000640066.1_1_9:123605558-123607345:+|ENST00000640391.1_1_9:123605558-123609583:+ PSMD5-AS1 lncRNA 9:123605559-123607307:+ 9 PSMD5-AS1 Pseudogene Pseudogene GN=PSMD5-AS1 E_20221201_NO30_400nL_HLAc1_1e7_directIP_titration_rep3_Slot2-3_1_3514 20mio-rep2 E_20221201_NO30_400nL_HLAc1_2e7_directIP_titration_rep3_Slot2-4_1_3518.16439.416.1 1 4 1 12.63 6.04 7.97 6.59 93.2 7 0 0 13100.0 Hydrogen 10 (A) 11 AEASPGGAVAW (R) 11 SPGSSPGAVGAE A E/A/S/P G G\A\V\A\W 54.5 6 4 3 0 0 unbiased 56.779427 1.475773 1015.478 1015.484 -0.0043 -4.3 2.0 4.0 6797.8 smORF_v4|nuORFdb_human PSMD5-AS1_9:123605559-123607307:+ PSMD5-AS1_9:123605559-123607307:+|ENST00000640066.1_1_9:123605558-123607345:+|ENST00000640391.1_1_9:123605558-123609583:+ PSMD5-AS1 lncRNA 9:123605559-123607307:+ 9 PSMD5-AS1 Pseudogene Pseudogene GN=PSMD5-AS1 E_20221201_NO30_400nL_HLAc1_2e7_directIP_titration_rep3_Slot2-4_1_3518 20mio-rep3 E_20221201_NO30_400nL_HLAc1_2e7_directIP_titration_rep4_Slot2-4_1_3519.16364.413.1 1 2 1 12.82 5.94 9.35 6.88 92.5 6 0 0 8810.0 Hydrogen 10 (A) 11 AEASPGGAVAW (R) 11 SPGSSPGAVGAE A E A/S/P G G\A\V\A\W 45.5 5 4 2 0 0 b-ion bias 56.521568 1.47739 1015.4768 1015.484 -0.0043 -4.3 1.5 4.0 6797.8 smORF_v4|nuORFdb_human PSMD5-AS1_9:123605559-123607307:+ PSMD5-AS1_9:123605559-123607307:+|ENST00000640066.1_1_9:123605558-123607345:+|ENST00000640391.1_1_9:123605558-123609583:+ PSMD5-AS1 lncRNA 9:123605559-123607307:+ 9 PSMD5-AS1 Pseudogene Pseudogene GN=PSMD5-AS1 E_20221201_NO30_400nL_HLAc1_2e7_directIP_titration_rep4_Slot2-4_1_3519 40mio-rep1 E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep1_Slot1-04_1_3496.16623.399.1 1 7 1 15.6 6.55 9.76 9.05 95.5 8 0 0 53000.0 Hydrogen 10 (A) 11 AEASPGGAVAW (R) 11 SPGSSPGAVGAE A E/A/S|P\G G\A\V\A\W 63.6 7 6 3 1 0 b-ion bias 57.420765 1.485928 1015.4808 1015.484 -0.0043 -4.3 -1.0999999999999996 4.0 6797.8 smORF_v4|nuORFdb_human PSMD5-AS1_9:123605559-123607307:+ PSMD5-AS1_9:123605559-123607307:+|ENST00000640066.1_1_9:123605558-123607345:+|ENST00000640391.1_1_9:123605558-123609583:+ PSMD5-AS1 lncRNA 9:123605559-123607307:+ 9 PSMD5-AS1 Pseudogene Pseudogene GN=PSMD5-AS1 E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep1_Slot1-04_1_3496 40mio-rep2 E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep2_Slot2-5_1_3520.16296.418.1 1 2 1 15.01 6.3 10.86 8.71 96.1 8 0 0 8240.0 Hydrogen 10 (A) 11 AEASPGGAVAW (R) 11 SPGSSPGAVGAE A E/A/S/P\G\G A\V\A\W 63.6 7 5 3 0 0 unbiased 56.291794 1.474795 1015.4825 1015.484 -0.0043 -4.3 0.6000000000000005 4.0 6797.8 smORF_v4|nuORFdb_human PSMD5-AS1_9:123605559-123607307:+ PSMD5-AS1_9:123605559-123607307:+|ENST00000640066.1_1_9:123605558-123607345:+|ENST00000640391.1_1_9:123605558-123609583:+ PSMD5-AS1 lncRNA 9:123605559-123607307:+ 9 PSMD5-AS1 Pseudogene Pseudogene GN=PSMD5-AS1 E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep2_Slot2-5_1_3520 40mio-rep3 E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep3_Slot2-5_1_3521.16306.414.1 1 2 1 12.29 4.85 8.89 7.44 91.2 7 0 0 11300.0 Hydrogen 10 (A) 11 AEASPGGAVAW (R) 11 SPGSSPGAVGAE A E/A/S/P G G\A\V\A\W 54.5 6 4 3 0 0 unbiased 56.328045 1.47723 1015.478 1015.484 -0.0043 -4.3 0.2999999999999998 4.0 6797.8 smORF_v4|nuORFdb_human PSMD5-AS1_9:123605559-123607307:+ PSMD5-AS1_9:123605559-123607307:+|ENST00000640066.1_1_9:123605558-123607345:+|ENST00000640391.1_1_9:123605558-123609583:+ PSMD5-AS1 lncRNA 9:123605559-123607307:+ 9 PSMD5-AS1 Pseudogene Pseudogene GN=PSMD5-AS1 E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep3_Slot2-5_1_3521 40mio-rep4 E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep4_Slot2-5_1_3522.16295.415.1 1 2 1 13.66 6.69 9.4 6.97 92.4 7 0 0 8240.0 Hydrogen 10 (A) 11 AEASPGGAVAW (R) 11 SPGSSPGAVGAE A E/A/S/P G G\A\V\A\W 54.5 6 4 3 0 0 unbiased 56.274456 1.476196 1015.4809 1015.484 -0.0043 -4.3 0.40000000000000036 4.0 6797.8 smORF_v4|nuORFdb_human PSMD5-AS1_9:123605559-123607307:+ PSMD5-AS1_9:123605559-123607307:+|ENST00000640066.1_1_9:123605558-123607345:+|ENST00000640391.1_1_9:123605558-123609583:+ PSMD5-AS1 lncRNA 9:123605559-123607307:+ 9 PSMD5-AS1 Pseudogene Pseudogene GN=PSMD5-AS1 E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep4_Slot2-5_1_3522 5mio-rep2 E_20221201_NO30_400nL_HLAc1_5e6_directIP_titration_rep2_Slot2-2_1_3510.16600.425.1 1 3 1 12.09 4.55 8.55 7.54 87.6 7 0 0 4780.0 Hydrogen 10 (A) 11 AEASPGGAVAW (R) 11 GPGAVAnATGAE A E/A/S|P G G\A\V\A\W 54.5 6 5 3 1 0 unbiased 57.335007 1.470296 1015.4782 1015.484 -0.0043 -4.3 4.3999999999999995 4.0 6797.8 smORF_v4|nuORFdb_human PSMD5-AS1_9:123605559-123607307:+ PSMD5-AS1_9:123605559-123607307:+|ENST00000640066.1_1_9:123605558-123607345:+|ENST00000640391.1_1_9:123605558-123609583:+ PSMD5-AS1 lncRNA 9:123605559-123607307:+ 9 PSMD5-AS1 Pseudogene Pseudogene GN=PSMD5-AS1 E_20221201_NO30_400nL_HLAc1_5e6_directIP_titration_rep2_Slot2-2_1_3510 20mio-rep2 E_20221201_NO30_400nL_HLAc1_2e7_directIP_titration_rep3_Slot2-4_1_3518.11705.652.1 1 1 1 10.48 8.68 1.02 1.8 82.4 5 0 0 2640.0 Hydrogen 54 (G) 8 AEDEAAAW (R) 8 AAEDEAADA A E D\E\A\A\A\W 62.5 5 5 0 0 0 b-ion bias 40.432278 1.334955 862.351 862.358 -0.0067 -7.8 -1.5 3.57 8811.4 nuORFdb_human ENST00000371142.8_1_10:98336578-98346758:- ENST00000371142.8_1_10:98336578-98346758:- TM9SF3 5' Overlap uORF 5' Overlap uORF GN=TM9SF3 5' Overlap uORF E_20221201_NO30_400nL_HLAc1_2e7_directIP_titration_rep3_Slot2-4_1_3518 40mio-rep2 E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep2_Slot2-5_1_3520.11521.649.1 1 2 1 9.95 5.09 2.36 4.86 85.4 5 0 0 7920.0 Hydrogen 54 (G) 8 AEDEAAAW (R) 8 DAAEAEAW A E D\E\A\A\A\W 62.5 5 5 0 0 0 b-ion bias 39.798702 1.336718 862.3563 862.358 -0.0017 -2.0 2.9000000000000004 3.57 8811.4 nuORFdb_human ENST00000371142.8_1_10:98336578-98346758:- ENST00000371142.8_1_10:98336578-98346758:- TM9SF3 5' Overlap uORF 5' Overlap uORF GN=TM9SF3 5' Overlap uORF E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep2_Slot2-5_1_3520 40mio-rep3 E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep3_Slot2-5_1_3521.11515.645.1 1 1 1 11.38 8.65 1.26 2.72 80.5 5 0 0 2740.0 Hydrogen 54 (G) 8 AEDEAAAW (R) 8 AAEDEAADA A E D|E\A\A\A\W 62.5 5 5 1 1 0 b-ion bias 39.781109 1.339288 862.3545 862.358 -0.0027 -3.2 1.3999999999999995 3.57 8811.4 nuORFdb_human ENST00000371142.8_1_10:98336578-98346758:- ENST00000371142.8_1_10:98336578-98346758:- TM9SF3 5' Overlap uORF 5' Overlap uORF GN=TM9SF3 5' Overlap uORF E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep3_Slot2-5_1_3521 1mio-rep1 E_20221201_NO30_400nL_HLAc1_1e6_directIP_titration_rep1_Slot1-06_1_3498.17876.1342.2 2 2 1 18.44 2.85 17.95 15.59 85.7 10 1 1 2710.0 Hydrogen 54 (G) 11 AEDEAAAWRSW (R) 11 AESSnPATLEGSAG A/E/D|E|A|A|A|W|R|S\W 100.0 11 8 9 7 0 dual b/y bias 61.738121 0.912717 646.2877 1291.57 -0.0015 -1.1 3.8000000000000003 4.14 8811.4 nuORFdb_human ENST00000371142.8_1_10:98336578-98346758:- ENST00000371142.8_1_10:98336578-98346758:- TM9SF3 5' Overlap uORF 5' Overlap uORF GN=TM9SF3 5' Overlap uORF E_20221201_NO30_400nL_HLAc1_1e6_directIP_titration_rep1_Slot1-06_1_3498 10mio-rep1 E_20221201_NO30_400nL_HLAc1_1e7_directIP_titration_rep1_Slot2-3_1_3512.17595.1351.2 2 2 1 17.92 4.16 13.96 13.76 91.8 9 1 1 6080.0 Hydrogen 54 (G) 11 AEDEAAAWRSW (R) 11 AEGEDVGWWRS A E/D|E|A|A|A|W|R|S\W 81.8 9 8 8 7 0 dual b/y bias 60.77919 0.907148 646.2826 1291.57 -0.0115 -8.9 -1.2000000000000002 4.14 8811.4 nuORFdb_human ENST00000371142.8_1_10:98336578-98346758:- ENST00000371142.8_1_10:98336578-98346758:- TM9SF3 5' Overlap uORF 5' Overlap uORF GN=TM9SF3 5' Overlap uORF E_20221201_NO30_400nL_HLAc1_1e7_directIP_titration_rep1_Slot2-3_1_3512 10mio-rep2 E_20221201_NO30_400nL_HLAc1_1e7_directIP_titration_rep2_Slot2-3_1_3513.17532.1347.2 2 2 1 16.26 3.19 11.63 13.07 90.8 9 1 1 3940.0 Hydrogen 54 (G) 11 AEDEAAAWRSW (R) 11 AESSnPATLEGSAG A E/D\E|A|A|A|W|R\S\W 81.8 9 8 6 5 0 dual b/y bias 60.560566 0.90921 646.2867 1291.57 -0.0035 -2.7 4.0 4.14 8811.4 nuORFdb_human ENST00000371142.8_1_10:98336578-98346758:- ENST00000371142.8_1_10:98336578-98346758:- TM9SF3 5' Overlap uORF 5' Overlap uORF GN=TM9SF3 5' Overlap uORF E_20221201_NO30_400nL_HLAc1_1e7_directIP_titration_rep2_Slot2-3_1_3513 10mio-rep3 E_20221201_NO30_400nL_HLAc1_1e7_directIP_titration_rep3_Slot2-3_1_3514.17499.1348.2 2 2 1 17.58 2.85 13.84 14.73 89.8 10 1 1 7470.0 Hydrogen 54 (G) 11 AEDEAAAWRSW (R) 11 AESSnPATLEGSAG A/E/D|E|A|A|A|W|R/S\W 100.0 11 7 9 6 0 dual b/y bias 60.446308 0.909002 646.2832 1291.57 -0.0115 -8.9 -2.6000000000000005 4.14 8811.4 nuORFdb_human ENST00000371142.8_1_10:98336578-98346758:- ENST00000371142.8_1_10:98336578-98346758:- TM9SF3 5' Overlap uORF 5' Overlap uORF GN=TM9SF3 5' Overlap uORF E_20221201_NO30_400nL_HLAc1_1e7_directIP_titration_rep3_Slot2-3_1_3514 20mio-rep1 E_20221201_NO30_400nL_HLAc1_2e7_directIP_titration_rep1_Slot2-4_1_3515.17486.1342.2 2 3 1 17.9 3.34 13.42 14.56 92.9 8 1 1 18900.0 Hydrogen 54 (G) 11 AEDEAAAWRSW (R) 11 AESSnPATLEGSAG A E/D|E|A|A|A|W|R|S W 63.6 7 7 8 7 0 dual b/y bias 60.390327 0.9127 646.283 1291.57 -0.0115 -8.9 -2.7 4.14 8811.4 nuORFdb_human ENST00000371142.8_1_10:98336578-98346758:- ENST00000371142.8_1_10:98336578-98346758:- TM9SF3 5' Overlap uORF 5' Overlap uORF GN=TM9SF3 5' Overlap uORF E_20221201_NO30_400nL_HLAc1_2e7_directIP_titration_rep1_Slot2-4_1_3515 20mio-rep2 E_20221201_NO30_400nL_HLAc1_2e7_directIP_titration_rep3_Slot2-4_1_3518.17583.1337.2 2 2 1 17.78 4.58 13.12 13.2 91.1 9 1 1 12300.0 Hydrogen 54 (G) 11 AEDEAAAWRSW (R) 11 AESSnPATLEGSAG A/E/D|E|A|A|A|W|R|S W 81.8 9 7 9 7 0 dual b/y bias 60.733681 0.915483 646.285 1291.57 -0.0075 -5.8 0.5 4.14 8811.4 nuORFdb_human ENST00000371142.8_1_10:98336578-98346758:- ENST00000371142.8_1_10:98336578-98346758:- TM9SF3 5' Overlap uORF 5' Overlap uORF GN=TM9SF3 5' Overlap uORF E_20221201_NO30_400nL_HLAc1_2e7_directIP_titration_rep3_Slot2-4_1_3518 20mio-rep3 E_20221201_NO30_400nL_HLAc1_2e7_directIP_titration_rep4_Slot2-4_1_3519.17513.1345.2 2 3 1 19.7 4.66 15.7 15.04 95.0 8 1 1 15200.0 Hydrogen 54 (G) 11 AEDEAAAWRSW (R) 11 AESSnPATLEGSAG A E/D|E|A|A|A|W|R|S W 63.6 7 7 8 7 0 dual b/y bias 60.488838 0.910456 646.2826 1291.57 -0.0115 -8.9 -3.1000000000000005 4.14 8811.4 nuORFdb_human ENST00000371142.8_1_10:98336578-98346758:- ENST00000371142.8_1_10:98336578-98346758:- TM9SF3 5' Overlap uORF 5' Overlap uORF GN=TM9SF3 5' Overlap uORF E_20221201_NO30_400nL_HLAc1_2e7_directIP_titration_rep4_Slot2-4_1_3519 40mio-rep1 E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep1_Slot1-04_1_3496.17768.1303.2 2 2 1 21.05 4.27 15.1 16.78 94.4 9 1 1 31700.0 Hydrogen 54 (G) 11 AEDEAAAWRSW (R) 11 AESSnPATLEGSAG A E/D|E|A|A|A|W|R|S\W 81.8 9 8 8 7 0 dual b/y bias 61.377476 0.936979 646.2864 1291.57 -0.0055 -4.2 -1.0 4.14 8811.4 nuORFdb_human ENST00000371142.8_1_10:98336578-98346758:- ENST00000371142.8_1_10:98336578-98346758:- TM9SF3 5' Overlap uORF 5' Overlap uORF GN=TM9SF3 5' Overlap uORF E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep1_Slot1-04_1_3496 40mio-rep2 E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep2_Slot2-5_1_3520.17405.1322.2 2 4 1 20.63 4.85 14.13 15.79 94.6 9 1 1 42300.0 Hydrogen 54 (G) 11 AEDEAAAWRSW (R) 11 AEGEDVGWWRS A E/D|E|A|A|A|W|R|S\W 81.8 9 8 8 7 0 dual b/y bias 60.124996 0.925281 646.2851 1291.57 -0.0075 -5.8 -0.8999999999999995 4.14 8811.4 nuORFdb_human ENST00000371142.8_1_10:98336578-98346758:- ENST00000371142.8_1_10:98336578-98346758:- TM9SF3 5' Overlap uORF 5' Overlap uORF GN=TM9SF3 5' Overlap uORF E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep2_Slot2-5_1_3520 40mio-rep3 E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep3_Slot2-5_1_3521.17439.1326.2 2 3 1 19.41 2.08 14.72 17.33 92.4 9 1 1 24900.0 Hydrogen 54 (G) 11 AEDEAAAWRSW (R) 11 AESSnPATLEGSAG A E/D|E|A|A|A|W|R|S\W 81.8 9 8 8 7 0 dual b/y bias 60.240891 0.922775 646.2856 1291.57 -0.0055 -4.2 0.39999999999999947 4.14 8811.4 nuORFdb_human ENST00000371142.8_1_10:98336578-98346758:- ENST00000371142.8_1_10:98336578-98346758:- TM9SF3 5' Overlap uORF 5' Overlap uORF GN=TM9SF3 5' Overlap uORF E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep3_Slot2-5_1_3521 40mio-rep4 E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep4_Slot2-5_1_3522.17407.1327.2 2 3 1 22.35 5.54 16.06 16.81 97.7 10 1 1 23000.0 Hydrogen 54 (G) 11 AEDEAAAWRSW (R) 11 AESSnPATLEGSAG A/E/D|E|A|A|A|W|R|S\W 100.0 11 8 9 7 0 dual b/y bias 60.115086 0.922308 646.2861 1291.57 -0.0055 -4.2 0.5 4.14 8811.4 nuORFdb_human ENST00000371142.8_1_10:98336578-98346758:- ENST00000371142.8_1_10:98336578-98346758:- TM9SF3 5' Overlap uORF 5' Overlap uORF GN=TM9SF3 5' Overlap uORF E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep4_Slot2-5_1_3522 5mio-rep1 E_20221201_NO30_400nL_HLAc1_5e6_directIP_titration_rep1_Slot1-37_1_3502.17854.1339.2 2 5 1 18.62 2.93 13.45 15.69 89.3 10 1 1 16800.0 Hydrogen 54 (G) 11 AEDEAAAWRSW (R) 11 AESSnPATLEGSAG A/E/D|E|A|A|A|W|R|S\W 100.0 11 8 9 7 0 dual b/y bias 61.669395 0.914535 646.2829 1291.57 -0.0115 -8.9 -1.9000000000000004 4.14 8811.4 nuORFdb_human ENST00000371142.8_1_10:98336578-98346758:- ENST00000371142.8_1_10:98336578-98346758:- TM9SF3 5' Overlap uORF 5' Overlap uORF GN=TM9SF3 5' Overlap uORF E_20221201_NO30_400nL_HLAc1_5e6_directIP_titration_rep1_Slot1-37_1_3502 5mio-rep2 E_20221201_NO30_400nL_HLAc1_5e6_directIP_titration_rep2_Slot2-2_1_3510.17733.1343.2 2 3 1 15.25 2.17 15.25 13.09 86.5 8 1 1 5580.0 Hydrogen 54 (G) 11 AEDEAAAWRSW (R) 11 AEGEDVGWWRS A E/D\E|A|A|A|W|R|S W 63.6 7 7 7 6 0 dual b/y bias 61.24992 0.912131 646.2799 1291.57 -0.0175 -13.5 -4.800000000000001 4.14 8811.4 nuORFdb_human ENST00000371142.8_1_10:98336578-98346758:- ENST00000371142.8_1_10:98336578-98346758:- TM9SF3 5' Overlap uORF 5' Overlap uORF GN=TM9SF3 5' Overlap uORF E_20221201_NO30_400nL_HLAc1_5e6_directIP_titration_rep2_Slot2-2_1_3510 5mio-rep3 E_20221201_NO30_400nL_HLAc1_5e6_directIP_titration_rep3_Slot2-2_1_3511.17733.1350.2 2 2 1 13.71 2.04 9.6 11.67 79.5 8 1 1 4310.0 Hydrogen 54 (G) 11 AEDEAAAWRSW (R) 11 AESSnPATLEGSAG A E/D\E|A|A|A|W|R-S W 63.6 7 6 6 5 1 dual b/y bias 61.250744 0.907476 646.2814 1291.57 -0.0155 -12.0 -3.8000000000000007 4.14 8811.4 nuORFdb_human ENST00000371142.8_1_10:98336578-98346758:- ENST00000371142.8_1_10:98336578-98346758:- TM9SF3 5' Overlap uORF 5' Overlap uORF GN=TM9SF3 5' Overlap uORF E_20221201_NO30_400nL_HLAc1_5e6_directIP_titration_rep3_Slot2-2_1_3511 40mio-rep4 E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep4_Slot2-5_1_3522.10742.1480.2 2 1 1 8.34 5.97 3.5 2.37 58.2 7 1 1 9340.0 Hydrogen 4 (R) 9 AEEGGRSLW (S) 9 AEEGIAAGGVM A/E/E|G|G R\S\L\W 77.8 7 5 4 2 0 unbiased 37.097916 0.82629 502.741 1004.48 -0.0049 -4.8 -0.09999999999999964 4.53 4071.7 nuORFdb_human ENST00000204517.10_1_16:4322677-4322779:- ENST00000204517.10_1_16:4322677-4322779:- TFAP4 5' Overlap uORF 5' Overlap uORF GN=TFAP4 5' Overlap uORF E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep4_Slot2-5_1_3522 1mio-rep1 E_20221201_NO30_400nL_HLAc1_1e6_directIP_titration_rep1_Slot1-06_1_3498.14118.1404.2 2 2 1 16.48 8.77 9.46 7.71 94.5 8 1 1 10100.0 Hydrogen 55 (A) 10 AEEPLAGRTW (R) 10 EAEPIELVET A E/E|P|L|A|G|R/T\W 80.0 8 6 7 5 0 dual b/y bias 48.76387 0.874049 565.2825 1129.564 -0.0069 -6.1 -1.1999999999999993 4.53 8015.0 nuORFdb_human ENST00000534515.1_1_11:67119307-67119526:- ENST00000534515.1_1_11:67119307-67119526:-|ENST00000529704.5_1_11:67119307-67120167:- POLD4 3' Overlap dORF 3' Overlap dORF GN=POLD4 3' Overlap dORF E_20221201_NO30_400nL_HLAc1_1e6_directIP_titration_rep1_Slot1-06_1_3498 1mio-rep2 E_20221201_NO30_400nL_HLAc1_1e6_directIP_titration_rep3_Slot2-1_1_3508.14108.1439.2 2 4 1 9.82 6.24 4.77 3.57 78.1 6 1 1 11100.0 Hydrogen 55 (A) 10 AEEPLAGRTW (R) 10 EAEPIELVET A E/E|P/L/A|G R T\W 50.0 5 3 5 2 0 unbiased 48.726513 0.851782 565.2818 1129.564 -0.0069 -6.1 2.200000000000001 4.53 8015.0 nuORFdb_human ENST00000534515.1_1_11:67119307-67119526:- ENST00000534515.1_1_11:67119307-67119526:-|ENST00000529704.5_1_11:67119307-67120167:- POLD4 3' Overlap dORF 3' Overlap dORF GN=POLD4 3' Overlap dORF E_20221201_NO30_400nL_HLAc1_1e6_directIP_titration_rep3_Slot2-1_1_3508 1mio-rep3 E_20221201_NO30_400nL_HLAc1_1e6_directIP_titration_rep4_Slot2-1_1_3509.13982.1423.2 2 3 1 13.97 8.18 8.58 5.8 89.2 8 1 1 6330.0 Hydrogen 55 (A) 10 AEEPLAGRTW (R) 10 EAEPIELVET A E/E|P/L|A|G/R/T\W 80.0 8 4 7 3 0 unbiased 48.288395 0.861964 565.2799 1129.564 -0.0109 -9.7 -1.6999999999999993 4.53 8015.0 nuORFdb_human ENST00000534515.1_1_11:67119307-67119526:- ENST00000534515.1_1_11:67119307-67119526:-|ENST00000529704.5_1_11:67119307-67120167:- POLD4 3' Overlap dORF 3' Overlap dORF GN=POLD4 3' Overlap dORF E_20221201_NO30_400nL_HLAc1_1e6_directIP_titration_rep4_Slot2-1_1_3509 10mio-rep1 E_20221201_NO30_400nL_HLAc1_1e7_directIP_titration_rep1_Slot2-3_1_3512.13829.1423.2 2 2 1 16.22 8.39 9.84 7.82 93.6 8 1 1 86000.0 Hydrogen 55 (A) 10 AEEPLAGRTW (R) 10 EAEPIELVET A E/E|P/L|A|G/R|T\W 80.0 8 5 7 4 0 dual b/y bias 47.77459 0.861783 565.2814 1129.564 -0.0089 -7.9 -0.20000000000000018 4.53 8015.0 nuORFdb_human ENST00000534515.1_1_11:67119307-67119526:- ENST00000534515.1_1_11:67119307-67119526:-|ENST00000529704.5_1_11:67119307-67120167:- POLD4 3' Overlap dORF 3' Overlap dORF GN=POLD4 3' Overlap dORF E_20221201_NO30_400nL_HLAc1_1e7_directIP_titration_rep1_Slot2-3_1_3512 10mio-rep2 E_20221201_NO30_400nL_HLAc1_1e7_directIP_titration_rep2_Slot2-3_1_3513.13752.1421.2 2 2 1 15.05 9.1 8.8 5.95 90.8 7 1 1 25000.0 Hydrogen 55 (A) 10 AEEPLAGRTW (R) 10 EAEPIELVET A E/E|P/L|A|G/R|T W 60.0 6 4 7 4 0 dual b/y bias 47.508217 0.863295 565.2817 1129.564 -0.0069 -6.1 0.6000000000000005 4.53 8015.0 nuORFdb_human ENST00000534515.1_1_11:67119307-67119526:- ENST00000534515.1_1_11:67119307-67119526:-|ENST00000529704.5_1_11:67119307-67120167:- POLD4 3' Overlap dORF 3' Overlap dORF GN=POLD4 3' Overlap dORF E_20221201_NO30_400nL_HLAc1_1e7_directIP_titration_rep2_Slot2-3_1_3513 10mio-rep3 E_20221201_NO30_400nL_HLAc1_1e7_directIP_titration_rep3_Slot2-3_1_3514.13730.1429.2 2 4 1 16.09 10.32 8.34 5.78 91.9 8 1 1 80300.0 Hydrogen 55 (A) 10 AEEPLAGRTW (R) 10 EAEPIELVET A E/E|P|L|A|G/R\T\W 80.0 8 6 6 4 0 dual b/y bias 47.434204 0.858221 565.2815 1129.564 -0.0069 -6.1 0.20000000000000018 4.53 8015.0 nuORFdb_human ENST00000534515.1_1_11:67119307-67119526:- ENST00000534515.1_1_11:67119307-67119526:-|ENST00000529704.5_1_11:67119307-67120167:- POLD4 3' Overlap dORF 3' Overlap dORF GN=POLD4 3' Overlap dORF E_20221201_NO30_400nL_HLAc1_1e7_directIP_titration_rep3_Slot2-3_1_3514 20mio-rep1 E_20221201_NO30_400nL_HLAc1_2e7_directIP_titration_rep1_Slot2-4_1_3515.13708.1405.2 2 3 1 19.28 10.44 11.39 8.84 95.5 9 1 1 196000.0 Hydrogen 55 (A) 10 AEEPLAGRTW (R) 10 EAEPIELVET A/E/E|P|L|A|G/R|T\W 100.0 10 6 8 5 0 dual b/y bias 47.346317 0.872911 565.2831 1129.564 -0.0049 -4.4 1.7999999999999998 4.53 8015.0 nuORFdb_human ENST00000534515.1_1_11:67119307-67119526:- ENST00000534515.1_1_11:67119307-67119526:-|ENST00000529704.5_1_11:67119307-67120167:- POLD4 3' Overlap dORF 3' Overlap dORF GN=POLD4 3' Overlap dORF E_20221201_NO30_400nL_HLAc1_2e7_directIP_titration_rep1_Slot2-4_1_3515 20mio-rep2 E_20221201_NO30_400nL_HLAc1_2e7_directIP_titration_rep3_Slot2-4_1_3518.13818.1402.2 2 3 1 21.23 11.42 11.34 9.81 94.5 9 1 1 139000.0 Hydrogen 55 (A) 10 AEEPLAGRTW (R) 10 EAEPIELVET A/E/E|P|L|A|G|R\T\W 100.0 10 7 7 5 0 dual b/y bias 47.727356 0.874799 565.2814 1129.564 -0.0089 -7.9 -1.6000000000000005 4.53 8015.0 nuORFdb_human ENST00000534515.1_1_11:67119307-67119526:- ENST00000534515.1_1_11:67119307-67119526:-|ENST00000529704.5_1_11:67119307-67120167:- POLD4 3' Overlap dORF 3' Overlap dORF GN=POLD4 3' Overlap dORF E_20221201_NO30_400nL_HLAc1_2e7_directIP_titration_rep3_Slot2-4_1_3518 20mio-rep3 E_20221201_NO30_400nL_HLAc1_2e7_directIP_titration_rep4_Slot2-4_1_3519.13719.1406.2 2 3 1 18.23 9.26 11.68 8.97 94.6 8 1 1 79200.0 Hydrogen 55 (A) 10 AEEPLAGRTW (R) 10 EAEPIELVET A E/E|P|L|A|G\R\T\W 80.0 8 7 5 4 0 dual b/y bias 47.388073 0.872894 565.2818 1129.564 -0.0069 -6.1 -0.2999999999999998 4.53 8015.0 nuORFdb_human ENST00000534515.1_1_11:67119307-67119526:- ENST00000534515.1_1_11:67119307-67119526:-|ENST00000529704.5_1_11:67119307-67120167:- POLD4 3' Overlap dORF 3' Overlap dORF GN=POLD4 3' Overlap dORF E_20221201_NO30_400nL_HLAc1_2e7_directIP_titration_rep4_Slot2-4_1_3519 40mio-rep1 E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep1_Slot1-04_1_3496.13984.1375.2 2 3 1 20.93 9.9 11.95 11.03 96.2 9 1 1 302000.0 Hydrogen 55 (A) 10 AEEPLAGRTW (R) 10 EAEPIELVET A/E|E|P|L|A|G/R|T\W 100.0 10 7 8 6 0 dual b/y bias 48.305435 0.891963 565.2837 1129.564 -0.0029 -2.6 0.6000000000000001 4.53 8015.0 nuORFdb_human ENST00000534515.1_1_11:67119307-67119526:- ENST00000534515.1_1_11:67119307-67119526:-|ENST00000529704.5_1_11:67119307-67120167:- POLD4 3' Overlap dORF 3' Overlap dORF GN=POLD4 3' Overlap dORF E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep1_Slot1-04_1_3496 40mio-rep2 E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep2_Slot2-5_1_3520.13697.1385.2 2 5 1 20.36 11.04 11.31 9.32 96.9 9 1 1 468000.0 Hydrogen 55 (A) 10 AEEPLAGRTW (R) 10 EAEPIELVET A/E/E|P|L|A|G|R|T\W 100.0 10 7 8 6 0 dual b/y bias 47.31572 0.886032 565.2839 1129.564 -0.0029 -2.6 2.3000000000000003 4.53 8015.0 nuORFdb_human ENST00000534515.1_1_11:67119307-67119526:- ENST00000534515.1_1_11:67119307-67119526:-|ENST00000529704.5_1_11:67119307-67120167:- POLD4 3' Overlap dORF 3' Overlap dORF GN=POLD4 3' Overlap dORF E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep2_Slot2-5_1_3520 40mio-rep3 E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep3_Slot2-5_1_3521.13697.1390.2 2 6 1 20.34 11.57 10.75 8.76 96.4 9 1 1 391000.0 Hydrogen 55 (A) 10 AEEPLAGRTW (R) 10 EAEPIELVET A/E/E|P|L|A|G|R|T\W 100.0 10 7 8 6 0 dual b/y bias 47.319118 0.882537 565.2826 1129.564 -0.0049 -4.4 0.1999999999999993 4.53 8015.0 nuORFdb_human ENST00000534515.1_1_11:67119307-67119526:- ENST00000534515.1_1_11:67119307-67119526:-|ENST00000529704.5_1_11:67119307-67120167:- POLD4 3' Overlap dORF 3' Overlap dORF GN=POLD4 3' Overlap dORF E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep3_Slot2-5_1_3521 40mio-rep4 E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep4_Slot2-5_1_3522.13676.1391.2 2 5 1 20.34 11.0 11.38 9.33 96.6 9 1 1 206000.0 Hydrogen 55 (A) 10 AEEPLAGRTW (R) 10 EAEPIELVET A/E/E|P|L|A|G|R|T\W 100.0 10 7 8 6 0 dual b/y bias 47.230465 0.882152 565.282 1129.564 -0.0069 -6.1 -1.3999999999999995 4.53 8015.0 nuORFdb_human ENST00000534515.1_1_11:67119307-67119526:- ENST00000534515.1_1_11:67119307-67119526:-|ENST00000529704.5_1_11:67119307-67120167:- POLD4 3' Overlap dORF 3' Overlap dORF GN=POLD4 3' Overlap dORF E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep4_Slot2-5_1_3522 5mio-rep1 E_20221201_NO30_400nL_HLAc1_5e6_directIP_titration_rep1_Slot1-37_1_3502.14053.1420.2 2 2 1 18.42 11.18 10.86 7.24 95.7 8 1 1 23900.0 Hydrogen 55 (A) 10 AEEPLAGRTW (R) 10 EAEPIELVET A E/E|P|L|A|G|R\T\W 80.0 8 7 6 5 0 dual b/y bias 48.541489 0.863743 565.2813 1129.564 -0.0089 -7.9 -0.9000000000000004 4.53 8015.0 nuORFdb_human ENST00000534515.1_1_11:67119307-67119526:- ENST00000534515.1_1_11:67119307-67119526:-|ENST00000529704.5_1_11:67119307-67120167:- POLD4 3' Overlap dORF 3' Overlap dORF GN=POLD4 3' Overlap dORF E_20221201_NO30_400nL_HLAc1_5e6_directIP_titration_rep1_Slot1-37_1_3502 5mio-rep3 E_20221201_NO30_400nL_HLAc1_5e6_directIP_titration_rep3_Slot2-2_1_3511.13971.1422.2 2 3 1 18.82 9.4 11.33 9.42 95.7 9 1 1 27800.0 Hydrogen 55 (A) 10 AEEPLAGRTW (R) 10 EAEPIELVET A/E/E|P|L|A|G/R|T\W 100.0 10 6 8 5 0 dual b/y bias 48.261555 0.862603 565.2792 1129.564 -0.0129 -11.4 -3.200000000000001 4.53 8015.0 nuORFdb_human ENST00000534515.1_1_11:67119307-67119526:- ENST00000534515.1_1_11:67119307-67119526:-|ENST00000529704.5_1_11:67119307-67120167:- POLD4 3' Overlap dORF 3' Overlap dORF GN=POLD4 3' Overlap dORF E_20221201_NO30_400nL_HLAc1_5e6_directIP_titration_rep3_Slot2-2_1_3511 40mio-rep1 E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep1_Slot1-04_1_3496.23959.1463.2 2 1 1 12.73 5.86 5.91 6.87 90.4 7 1 1 5380.0 Hydrogen 21 (S) 9 AEFGLRLGL (A) 9 AEFLNIRI A E/F|G/L|R\L\G\L 77.8 7 5 4 2 0 unbiased 82.767616 0.836526 488.2827 975.562 -0.0035 -3.6 -0.3999999999999999 6.05 8869.1 smORF_v4|nuORFdb_human FBXO5_6:153304369-153296756:- FBXO5_6:153304369-153296756:-|ENST00000367241.3_1_6:153296752-153304655:- FBXO5 overlapping.uORF 6:153304369-153296756:- 6 FBXO5 5' Overlap uORF 5' Overlap uORF GN=FBXO5 E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep1_Slot1-04_1_3496 10mio-rep1 E_20221201_NO30_400nL_HLAc1_1e7_directIP_titration_rep1_Slot2-3_1_3512.10943.799.1 1 1 1 10.5 6.69 3.61 3.81 82.5 7 0 0 3040.0 Hydrogen 2 (L) 9 AEGGPAPGL (L) 9 qQTAAPLG A E/G/G/P-A|P\G\L 77.8 7 3 4 1 1 unbiased 37.805988 1.246742 768.3831 768.389 -0.0056 -7.3 0.40000000000000036 4.0 8439.3 nuORFdb_human ENST00000521825.1_1_8:143353010-143361338:- ENST00000521825.1_1_8:143353010-143361338:- TSNARE1 ncRNA Processed Transcript lncRNA GN=TSNARE1 ncRNA Processed Transcript E_20221201_NO30_400nL_HLAc1_1e7_directIP_titration_rep1_Slot2-3_1_3512 10mio-rep2 E_20221201_NO30_400nL_HLAc1_1e7_directIP_titration_rep2_Slot2-3_1_3513.10911.795.1 1 1 1 12.07 8.29 4.51 3.78 85.2 8 0 0 2430.0 Hydrogen 2 (L) 9 AEGGPAPGL (L) 9 EQGPAGLP A/E/G/G|P\A|P\G-L 100.0 9 4 5 2 1 unbiased 37.69669 1.248814 768.3817 768.389 -0.0066 -8.6 -1.8999999999999995 4.0 8439.3 nuORFdb_human ENST00000521825.1_1_8:143353010-143361338:- ENST00000521825.1_1_8:143353010-143361338:- TSNARE1 ncRNA Processed Transcript lncRNA GN=TSNARE1 ncRNA Processed Transcript E_20221201_NO30_400nL_HLAc1_1e7_directIP_titration_rep2_Slot2-3_1_3513 10mio-rep3 E_20221201_NO30_400nL_HLAc1_1e7_directIP_titration_rep3_Slot2-3_1_3514.10886.803.1 1 1 1 9.01 5.75 3.4 3.25 80.3 7 0 0 3490.0 Hydrogen 2 (L) 9 AEGGPAPGL (L) 9 qQTAAPLG A E/G/G/P\A|P\G\L 77.8 7 4 4 1 0 unbiased 37.610878 1.244224 768.3841 768.389 -0.0046 -6.0 0.2999999999999998 4.0 8439.3 nuORFdb_human ENST00000521825.1_1_8:143353010-143361338:- ENST00000521825.1_1_8:143353010-143361338:- TSNARE1 ncRNA Processed Transcript lncRNA GN=TSNARE1 ncRNA Processed Transcript E_20221201_NO30_400nL_HLAc1_1e7_directIP_titration_rep3_Slot2-3_1_3514 20mio-rep1 E_20221201_NO30_400nL_HLAc1_2e7_directIP_titration_rep1_Slot2-4_1_3515.10812.797.1 1 3 1 11.91 7.61 4.09 4.3 81.8 8 0 0 8360.0 Hydrogen 2 (L) 9 AEGGPAPGL (L) 9 APGEGAPGL A/E/G/G/P|A|P\G\L 100.0 9 4 6 2 0 unbiased 37.344845 1.247646 768.3848 768.389 -0.0036 -4.7 1.5 4.0 8439.3 nuORFdb_human ENST00000521825.1_1_8:143353010-143361338:- ENST00000521825.1_1_8:143353010-143361338:- TSNARE1 ncRNA Processed Transcript lncRNA GN=TSNARE1 ncRNA Processed Transcript E_20221201_NO30_400nL_HLAc1_2e7_directIP_titration_rep1_Slot2-4_1_3515 20mio-rep2 E_20221201_NO30_400nL_HLAc1_2e7_directIP_titration_rep3_Slot2-4_1_3518.10974.791.1 1 1 1 10.33 6.64 2.97 3.69 76.9 8 0 0 3390.0 Hydrogen 2 (L) 9 AEGGPAPGL (L) 9 APGEGAPGL A/E/G/G/P\A|P\G\L 100.0 9 4 5 1 0 unbiased 37.905518 1.25127 768.3812 768.389 -0.0076 -9.9 -3.6000000000000005 4.0 8439.3 nuORFdb_human ENST00000521825.1_1_8:143353010-143361338:- ENST00000521825.1_1_8:143353010-143361338:- TSNARE1 ncRNA Processed Transcript lncRNA GN=TSNARE1 ncRNA Processed Transcript E_20221201_NO30_400nL_HLAc1_2e7_directIP_titration_rep3_Slot2-4_1_3518 20mio-rep3 E_20221201_NO30_400nL_HLAc1_2e7_directIP_titration_rep4_Slot2-4_1_3519.10851.792.1 1 1 1 11.06 8.32 3.47 2.74 83.1 7 0 0 4510.0 Hydrogen 2 (L) 9 AEGGPAPGL (L) 9 EQGPAGLP A E/G/G/P|A|P\G-L 77.8 7 3 5 2 1 unbiased 37.485867 1.250862 768.3852 768.389 -0.0036 -4.7 1.0999999999999996 4.0 8439.3 nuORFdb_human ENST00000521825.1_1_8:143353010-143361338:- ENST00000521825.1_1_8:143353010-143361338:- TSNARE1 ncRNA Processed Transcript lncRNA GN=TSNARE1 ncRNA Processed Transcript E_20221201_NO30_400nL_HLAc1_2e7_directIP_titration_rep4_Slot2-4_1_3519 40mio-rep1 E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep1_Slot1-04_1_3496.11135.775.1 1 2 1 12.98 8.66 4.54 4.32 86.5 7 0 0 15200.0 Hydrogen 2 (L) 9 AEGGPAPGL (L) 9 GEGAPAGPL A E/G/G|P|A|P\G\L 77.8 7 5 5 3 0 dual b/y bias 38.4655 1.260928 768.3857 768.389 -0.0026 -3.4 -0.19999999999999973 4.0 8439.3 nuORFdb_human ENST00000521825.1_1_8:143353010-143361338:- ENST00000521825.1_1_8:143353010-143361338:- TSNARE1 ncRNA Processed Transcript lncRNA GN=TSNARE1 ncRNA Processed Transcript E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep1_Slot1-04_1_3496 40mio-rep2 E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep2_Slot2-5_1_3520.10829.783.1 1 2 1 10.33 7.72 3.34 2.62 78.4 7 0 0 8820.0 Hydrogen 2 (L) 9 AEGGPAPGL (L) 9 GEGAPAGPL A E/G/G|P\A|P\G\L 77.8 7 5 4 2 0 unbiased 37.410137 1.256385 768.3821 768.389 -0.0066 -8.6 -3.6999999999999993 4.0 8439.3 nuORFdb_human ENST00000521825.1_1_8:143353010-143361338:- ENST00000521825.1_1_8:143353010-143361338:- TSNARE1 ncRNA Processed Transcript lncRNA GN=TSNARE1 ncRNA Processed Transcript E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep2_Slot2-5_1_3520 40mio-rep3 E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep3_Slot2-5_1_3521.10828.788.1 1 2 1 11.8 8.2 4.21 3.6 79.9 8 0 0 9680.0 Hydrogen 2 (L) 9 AEGGPAPGL (L) 9 GEGAPAGPL A/E/G/G/P|A|P\G\L 100.0 9 4 6 2 0 unbiased 37.408432 1.253162 768.3856 768.389 -0.0026 -3.4 1.1999999999999997 4.0 8439.3 nuORFdb_human ENST00000521825.1_1_8:143353010-143361338:- ENST00000521825.1_1_8:143353010-143361338:- TSNARE1 ncRNA Processed Transcript lncRNA GN=TSNARE1 ncRNA Processed Transcript E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep3_Slot2-5_1_3521 40mio-rep4 E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep4_Slot2-5_1_3522.10855.819.1 1 3 1 11.48 8.71 3.26 2.76 81.2 7 0 0 8450.0 Hydrogen 2 (L) 9 AEGGPAPGL (L) 9 EGAPGAPIG A E/G/G/P|A|P\G\L 77.8 7 4 5 2 0 unbiased 37.487961 1.234459 768.3874 768.389 -0.0016 -2.1 2.6 4.0 8439.3 nuORFdb_human ENST00000521825.1_1_8:143353010-143361338:- ENST00000521825.1_1_8:143353010-143361338:- TSNARE1 ncRNA Processed Transcript lncRNA GN=TSNARE1 ncRNA Processed Transcript E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep4_Slot2-5_1_3522 5mio-rep1 E_20221201_NO30_400nL_HLAc1_5e6_directIP_titration_rep1_Slot1-37_1_3502.11177.793.1 1 1 1 8.83 5.97 2.74 2.86 67.0 7 0 0 2070.0 Hydrogen 2 (L) 9 AEGGPAPGL (L) 9 GQEPAPGI A E/G/G/P\A|P\G\L 77.8 7 4 4 1 0 unbiased 38.611076 1.250261 768.3822 768.389 -0.0066 -8.6 -1.5999999999999996 4.0 8439.3 nuORFdb_human ENST00000521825.1_1_8:143353010-143361338:- ENST00000521825.1_1_8:143353010-143361338:- TSNARE1 ncRNA Processed Transcript lncRNA GN=TSNARE1 ncRNA Processed Transcript E_20221201_NO30_400nL_HLAc1_5e6_directIP_titration_rep1_Slot1-37_1_3502 40mio-rep1 E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep1_Slot1-04_1_3496.13704.614.1 1 1 1 11.3 7.73 4.25 3.57 80.7 6 0 0 2850.0 Hydrogen 11 (R) 9 AEGPAGQPW (R) 9 AGQPEAGPW A E/G/P A\G\Q|P\W 55.6 5 4 3 1 0 unbiased 47.337891 1.357873 912.4198 912.421 -0.001 -1.1 2.1 4.0 16053.1 nuORFdb_human ENST00000562767.2_3_15:68486355-68521975:- ENST00000562767.2_3_15:68486355-68521975:- RP11-315D16.2 3' Overlap dORF 3' Overlap dORF GN=RP11-315D16.2 3' Overlap dORF E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep1_Slot1-04_1_3496 20mio-rep1 E_20221201_NO30_400nL_HLAc1_2e7_directIP_titration_rep1_Slot2-4_1_3515.5847.1723.3 3 2 1 7.86 3.26 3.93 4.6 83.6 6 1 1 7620.0 Hydrogen 3 (L) 9 AEIHPKAGL (Q) 9 APNPAPQKL A/E/I/H/P/K A G-L 66.7 6 0 5 0 1 y-ion bias 20.203733 0.67223 312.5136 935.531 -0.0034 -3.7 2.5 6.79 9844.3 smORF_v4|nuORFdb_human CRNDE_16:54952999-54957547:- CRNDE_16:54952999-54957547:-|ENST00000613942.2_2_16:54927299-54959048:-|ENST00000501177.7_1_16:54953001-54959048:-|TCONS_00024662_16:54954217-54959048:-|ENST00000559598.6_1_16:54954310-54959048:-|ENST00000560912.5_1_16:54954310-54959048:-|ENST00000558952.1_1_16:54957460-54959048:- CRNDE lncRNA 16:54952999-54957547:- 16 CRNDE lincRNA lncRNA GN=CRNDE E_20221201_NO30_400nL_HLAc1_2e7_directIP_titration_rep1_Slot2-4_1_3515 40mio-rep1 E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep1_Slot1-04_1_3496.6123.1493.2 2 3 1 10.27 5.41 5.82 4.86 78.1 7 1 1 25900.0 Hydrogen 3 (L) 9 AEIHPKAGL (Q) 9 AELKAQLY A/E/I/H/P/K\A-G\L 100.0 9 2 5 0 1 y-ion bias 21.160675 0.818194 468.2656 935.531 -0.0062 -6.6 -3.3999999999999995 6.79 9844.3 smORF_v4|nuORFdb_human CRNDE_16:54952999-54957547:- CRNDE_16:54952999-54957547:-|ENST00000613942.2_2_16:54927299-54959048:-|ENST00000501177.7_1_16:54953001-54959048:-|TCONS_00024662_16:54954217-54959048:-|ENST00000559598.6_1_16:54954310-54959048:-|ENST00000560912.5_1_16:54954310-54959048:-|ENST00000558952.1_1_16:54957460-54959048:- CRNDE lncRNA 16:54952999-54957547:- 16 CRNDE lincRNA lncRNA GN=CRNDE E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep1_Slot1-04_1_3496 40mio-rep3 E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep3_Slot2-5_1_3521.5869.1700.3 3 2 1 8.6 5.21 2.6 3.39 88.2 6 1 1 16300.0 Hydrogen 3 (L) 9 AEIHPKAGL (Q) 9 qKLnKHLA A/E/I/H/P K\A G-L 55.6 5 1 4 0 1 y-ion bias 20.278093 0.686906 312.513 935.531 -0.0064 -6.9 -2.3000000000000007 6.79 9844.3 smORF_v4|nuORFdb_human CRNDE_16:54952999-54957547:- CRNDE_16:54952999-54957547:-|ENST00000613942.2_2_16:54927299-54959048:-|ENST00000501177.7_1_16:54953001-54959048:-|TCONS_00024662_16:54954217-54959048:-|ENST00000559598.6_1_16:54954310-54959048:-|ENST00000560912.5_1_16:54954310-54959048:-|ENST00000558952.1_1_16:54957460-54959048:- CRNDE lncRNA 16:54952999-54957547:- 16 CRNDE lincRNA lncRNA GN=CRNDE E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep3_Slot2-5_1_3521 40mio-rep4 E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep4_Slot2-5_1_3522.5872.1501.2 2 4 1 11.18 5.32 5.92 5.86 74.3 6 1 1 33000.0 Hydrogen 3 (L) 9 AEIHPKAGL (Q) 9 EALHPQLK A E/I|H/P K\A\G\L 55.6 5 4 3 1 0 unbiased 20.285748 0.812642 468.2673 935.531 -0.0042 -4.5 0.20000000000000018 6.79 9844.3 smORF_v4|nuORFdb_human CRNDE_16:54952999-54957547:- CRNDE_16:54952999-54957547:-|ENST00000613942.2_2_16:54927299-54959048:-|ENST00000501177.7_1_16:54953001-54959048:-|TCONS_00024662_16:54954217-54959048:-|ENST00000559598.6_1_16:54954310-54959048:-|ENST00000560912.5_1_16:54954310-54959048:-|ENST00000558952.1_1_16:54957460-54959048:- CRNDE lncRNA 16:54952999-54957547:- 16 CRNDE lincRNA lncRNA GN=CRNDE E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep4_Slot2-5_1_3522 20mio-rep1 E_20221201_NO30_400nL_HLAc1_2e7_directIP_titration_rep1_Slot2-4_1_3515.12189.1540.2 2 1 1 9.04 6.51 4.53 2.53 67.0 5 1 1 2250.0 M41m Hydrogen 38 (D) 8 AEImGKLL (A) 8 AELGKmLL A E/I/m|G K|L\L 50.0 4 3 4 2 0 unbiased m:Oxidized methionine 42.1017 0.788068 445.7498 874.507 15.9861 -9.9 -3.7 6.05 5643.4 smORF_v4|nuORFdb_human SEC31A_4:83821320-83821376:- SEC31A_4:83821320-83821376:-|THAP9-AS1_4:83821487-83821376:-|ENST00000355196.6_1_4:83821322-83821651:-|ENST00000505028.5_1_4:83821319-83821548:- SEC31A uORF 4:83821320-83821376:- 4 SEC31A 5' uORF 5' uORF GN=SEC31A E_20221201_NO30_400nL_HLAc1_2e7_directIP_titration_rep1_Slot2-4_1_3515 40mio-rep2 E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep2_Slot2-5_1_3520.12318.1533.2 2 1 1 10.15 7.76 3.47 2.39 55.2 6 1 1 5840.0 M41m Hydrogen 38 (D) 8 AEImGKLL (A) 8 AELGKmLL A/E/I/m|G K/L\L 75.0 6 2 5 1 0 y-ion bias m:Oxidized methionine 42.553452 0.792805 445.7539 874.507 15.9941 -0.9 4.0 6.05 5643.4 smORF_v4|nuORFdb_human SEC31A_4:83821320-83821376:- SEC31A_4:83821320-83821376:-|THAP9-AS1_4:83821487-83821376:-|ENST00000355196.6_1_4:83821322-83821651:-|ENST00000505028.5_1_4:83821319-83821548:- SEC31A uORF 4:83821320-83821376:- 4 SEC31A 5' uORF 5' uORF GN=SEC31A E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep2_Slot2-5_1_3520 40mio-rep3 E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep3_Slot2-5_1_3521.12337.1542.2 2 1 1 7.98 5.65 3.41 2.34 56.7 6 1 1 4330.0 M41m Hydrogen 38 (D) 8 AEImGKLL (A) 8 AELGKmLL A/E/I|m|G K|L\L 75.0 6 4 5 3 0 dual b/y bias m:Oxidized methionine 42.623253 0.787324 445.7519 874.507 15.9901 -5.4 -0.8000000000000007 6.05 5643.4 smORF_v4|nuORFdb_human SEC31A_4:83821320-83821376:- SEC31A_4:83821320-83821376:-|THAP9-AS1_4:83821487-83821376:-|ENST00000355196.6_1_4:83821322-83821651:-|ENST00000505028.5_1_4:83821319-83821548:- SEC31A uORF 4:83821320-83821376:- 4 SEC31A 5' uORF 5' uORF GN=SEC31A E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep3_Slot2-5_1_3521 40mio-rep1 E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep1_Slot1-04_1_3496.26098.1340.2 2 1 1 12.99 2.69 8.65 10.3 82.9 8 1 1 4250.0 Hydrogen 38 (D) 10 AEIMGKLLAW (L) 10 AEISQAARKTG A E/I|M|G/K\L\L|A\W 80.0 8 6 5 3 0 unbiased 90.159927 0.913624 566.3134 1131.623 -0.0044 -3.8 -0.5999999999999996 6.05 5643.4 smORF_v4|nuORFdb_human SEC31A_4:83821320-83821376:- SEC31A_4:83821320-83821376:-|THAP9-AS1_4:83821487-83821376:-|ENST00000355196.6_1_4:83821322-83821651:-|ENST00000505028.5_1_4:83821319-83821548:- SEC31A uORF 4:83821320-83821376:- 4 SEC31A 5' uORF 5' uORF GN=SEC31A E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep1_Slot1-04_1_3496 40mio-rep1 E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep1_Slot1-04_1_3496.16732.1311.2 2 2 1 17.81 6.58 10.19 11.23 87.8 9 1 2 8750.0 Hydrogen 12 (L) 10 AELLFSAKKF (F) 10 EALLKKDHIS A/E/L|L|F/S/A/K/K\F 100.0 10 3 8 2 0 y-ion bias 57.797112 0.932095 577.3333 1153.662 -0.0028 -2.5 0.7000000000000002 8.64 4239.0 nuORFdb_human ENST00000564657.1_3_16:75656550-75656655:- ENST00000564657.1_3_16:75656550-75656655:- ADAT1 5' uORF 5' uORF GN=ADAT1 5' uORF E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep1_Slot1-04_1_3496 40mio-rep1 E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep1_Slot1-04_1_3496.13465.498.1 1 2 1 13.7 6.51 8.75 7.19 75.3 7 0 0 9720.0 Hydrogen 18 (V) 9 AEQLGTQGL (N) 9 VSSPAGTQGL A E/Q|L|G|T|Q\G\L 77.8 7 6 5 4 0 dual b/y bias 46.512756 1.427088 916.4708 916.473 -0.0024 -2.7 0.5 4.0 13833.7 nuORFdb_human ENST00000278893.11_1_11:62469959-62473679:- ENST00000278893.11_1_11:62469959-62473679:- BSCL2 Out-of-Frame Out-of-Frame GN=BSCL2 Out-of-Frame E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep1_Slot1-04_1_3496 40mio-rep2 E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep2_Slot2-5_1_3520.13178.521.1 1 1 1 11.64 4.73 7.52 6.91 60.2 7 0 0 3410.0 Hydrogen 18 (V) 9 AEQLGTQGL (N) 9 GAEALSQAVA A E/Q|L|G|T|Q\G\L 77.8 7 6 5 4 0 dual b/y bias 45.523296 1.413352 916.468 916.473 -0.0054 -5.9 -1.0 4.0 13833.7 nuORFdb_human ENST00000278893.11_1_11:62469959-62473679:- ENST00000278893.11_1_11:62469959-62473679:- BSCL2 Out-of-Frame Out-of-Frame GN=BSCL2 Out-of-Frame E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep2_Slot2-5_1_3520 10mio-rep2 E_20221201_NO30_400nL_HLAc1_1e7_directIP_titration_rep2_Slot2-3_1_3513.18413.570.1 1 1 1 8.9 5.72 3.18 3.18 70.9 5 0 0 3800.0 Hydrogen 15 (A) 9 AESATALGW (R) 9 GATETQLW A E S A/T|A|L\G\W 55.6 5 4 3 2 0 unbiased 63.602859 1.384501 905.4302 905.436 -0.0063 -7.0 -0.2999999999999998 4.0 3827.1 smORF_v4|nuORFdb_human PPM1B_2:44396153-44396271:+ PPM1B_2:44396153-44396271:+|ENST00000419807.5_3_2:44396152-44396269:+ PPM1B uORF 2:44396153-44396271:+ 2 PPM1B 5' uORF 5' uORF GN=PPM1B E_20221201_NO30_400nL_HLAc1_1e7_directIP_titration_rep2_Slot2-3_1_3513 20mio-rep1 E_20221201_NO30_400nL_HLAc1_2e7_directIP_titration_rep1_Slot2-4_1_3515.18359.565.1 1 2 1 7.45 3.71 5.59 3.73 66.1 6 0 0 16600.0 Hydrogen 15 (A) 9 AESATALGW (R) 9 GATETQLW A E/S A/T|A\L\G\W 55.6 5 4 3 1 0 unbiased 63.404018 1.387405 905.4314 905.436 -0.0053 -5.9 0.2999999999999998 4.0 3827.1 smORF_v4|nuORFdb_human PPM1B_2:44396153-44396271:+ PPM1B_2:44396153-44396271:+|ENST00000419807.5_3_2:44396152-44396269:+ PPM1B uORF 2:44396153-44396271:+ 2 PPM1B 5' uORF 5' uORF GN=PPM1B E_20221201_NO30_400nL_HLAc1_2e7_directIP_titration_rep1_Slot2-4_1_3515 20mio-rep2 E_20221201_NO30_400nL_HLAc1_2e7_directIP_titration_rep3_Slot2-4_1_3518.18445.566.1 1 1 1 9.2 6.06 4.78 3.14 68.3 5 0 0 5110.0 Hydrogen 15 (A) 9 AESATALGW (R) 9 GATETQLW A E S A|T|A|L\G\W 55.6 5 5 3 3 0 dual b/y bias 63.71032 1.386841 905.4318 905.436 -0.0043 -4.8 1.5 4.0 3827.1 smORF_v4|nuORFdb_human PPM1B_2:44396153-44396271:+ PPM1B_2:44396153-44396271:+|ENST00000419807.5_3_2:44396152-44396269:+ PPM1B uORF 2:44396153-44396271:+ 2 PPM1B 5' uORF 5' uORF GN=PPM1B E_20221201_NO30_400nL_HLAc1_2e7_directIP_titration_rep3_Slot2-4_1_3518 20mio-rep3 E_20221201_NO30_400nL_HLAc1_2e7_directIP_titration_rep4_Slot2-4_1_3519.18396.561.1 1 1 1 7.63 4.82 4.16 2.81 66.9 5 0 0 6320.0 Hydrogen 15 (A) 9 AESATALGW (R) 9 GATETQLW A E S A|T|A\L\G\W 55.6 5 5 2 2 0 b-ion bias 63.536556 1.389383 905.4333 905.436 -0.0033 -3.7 2.0999999999999996 4.0 3827.1 smORF_v4|nuORFdb_human PPM1B_2:44396153-44396271:+ PPM1B_2:44396153-44396271:+|ENST00000419807.5_3_2:44396152-44396269:+ PPM1B uORF 2:44396153-44396271:+ 2 PPM1B 5' uORF 5' uORF GN=PPM1B E_20221201_NO30_400nL_HLAc1_2e7_directIP_titration_rep4_Slot2-4_1_3519 40mio-rep1 E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep1_Slot1-04_1_3496.18652.540.1 1 2 1 9.46 5.6 6.17 3.86 73.5 5 0 0 46400.0 Hydrogen 15 (A) 9 AESATALGW (R) 9 GATETQLW A E S A|T|A\L\G\W 55.6 5 5 2 2 0 b-ion bias 64.4338 1.402418 905.4326 905.436 -0.0033 -3.7 -0.5 4.0 3827.1 smORF_v4|nuORFdb_human PPM1B_2:44396153-44396271:+ PPM1B_2:44396153-44396271:+|ENST00000419807.5_3_2:44396152-44396269:+ PPM1B uORF 2:44396153-44396271:+ 2 PPM1B 5' uORF 5' uORF GN=PPM1B E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep1_Slot1-04_1_3496 40mio-rep2 E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep2_Slot2-5_1_3520.18309.558.1 1 1 1 8.85 5.59 4.82 3.26 68.6 5 0 0 5080.0 Hydrogen 15 (A) 9 AESATALGW (R) 9 GATETQLW A E S A/T|A|L\G\W 55.6 5 4 3 2 0 unbiased 63.249523 1.391244 905.4308 905.436 -0.0053 -5.9 -1.0 4.0 3827.1 smORF_v4|nuORFdb_human PPM1B_2:44396153-44396271:+ PPM1B_2:44396153-44396271:+|ENST00000419807.5_3_2:44396152-44396269:+ PPM1B uORF 2:44396153-44396271:+ 2 PPM1B 5' uORF 5' uORF GN=PPM1B E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep2_Slot2-5_1_3520 40mio-rep3 E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep3_Slot2-5_1_3521.18350.560.1 1 2 1 7.43 5.4 3.78 2.03 66.5 5 0 0 27400.0 Hydrogen 15 (A) 9 AESATALGW (R) 9 TQETALGEG A E S A/T|A|L\G\W 55.6 5 4 3 2 0 unbiased 63.389301 1.39017 905.4314 905.436 -0.0053 -5.9 -1.3000000000000007 4.0 3827.1 smORF_v4|nuORFdb_human PPM1B_2:44396153-44396271:+ PPM1B_2:44396153-44396271:+|ENST00000419807.5_3_2:44396152-44396269:+ PPM1B uORF 2:44396153-44396271:+ 2 PPM1B 5' uORF 5' uORF GN=PPM1B E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep3_Slot2-5_1_3521 40mio-rep4 E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep4_Slot2-5_1_3522.18320.558.1 1 2 1 8.63 5.4 4.36 3.23 68.4 5 0 0 31500.0 Hydrogen 15 (A) 9 AESATALGW (R) 9 GATETQLW A E S A/T|A|L\G\W 55.6 5 4 3 2 0 unbiased 63.268066 1.391386 905.4315 905.436 -0.0043 -4.8 -0.09999999999999964 4.0 3827.1 smORF_v4|nuORFdb_human PPM1B_2:44396153-44396271:+ PPM1B_2:44396153-44396271:+|ENST00000419807.5_3_2:44396152-44396269:+ PPM1B uORF 2:44396153-44396271:+ 2 PPM1B 5' uORF 5' uORF GN=PPM1B E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep4_Slot2-5_1_3522 40mio-rep4 E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep4_Slot2-5_1_3522.14262.1397.2 2 1 1 7.51 7.51 7.51 51.5 7 1 1 12400.0 Hydrogen 12 (P) 11 AESDLGVKGWG (-) 11 A E/S/D|L|G/V|K G W\G 54.5 6 4 6 3 0 unbiased 49.256065 0.878307 559.7751 1118.548 -0.0049 -4.4 0.2999999999999998 4.37 2235.5 nuORFdb_human ENST00000375499.7_1_1:17380528-17380597:- ENST00000375499.7_1_1:17380528-17380597:- SDHB 5' uORF 5' uORF GN=SDHB 5' uORF E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep4_Slot2-5_1_3522 10mio-rep1 E_20221201_NO30_400nL_HLAc1_1e7_directIP_titration_rep1_Slot2-3_1_3512.8903.1590.2 2 2 1 12.08 10.02 3.79 2.07 81.9 7 1 1 8370.0 Hydrogen 11 (A) 9 AESGAIRAL (-) 9 AESAGIALR A/E/S|G/A|I/R A\L 77.8 7 3 6 2 0 y-ion bias 30.760805 0.756721 444.2472 887.495 -0.0078 -8.8 -1.1000000000000005 6.05 1902.2 nuORFdb_human ENST00000487431.1_1_9:127624149-127624209:- ENST00000487431.1_1_9:127624149-127624209:- RPL35 ncRNA Retained Intron lncRNA GN=RPL35 ncRNA Retained Intron E_20221201_NO30_400nL_HLAc1_1e7_directIP_titration_rep1_Slot2-3_1_3512 10mio-rep2 E_20221201_NO30_400nL_HLAc1_1e7_directIP_titration_rep2_Slot2-3_1_3513.8856.1591.2 2 3 1 9.32 7.18 4.22 2.14 71.0 7 1 1 13100.0 Hydrogen 11 (A) 9 AESGAIRAL (-) 9 AESAGIALR A/E/S/G/A/I/R\A-L 100.0 9 1 6 0 1 y-ion bias 30.597784 0.756182 444.2477 887.495 -0.0058 -6.5 0.20000000000000018 6.05 1902.2 nuORFdb_human ENST00000487431.1_1_9:127624149-127624209:- ENST00000487431.1_1_9:127624149-127624209:- RPL35 ncRNA Retained Intron lncRNA GN=RPL35 ncRNA Retained Intron E_20221201_NO30_400nL_HLAc1_1e7_directIP_titration_rep2_Slot2-3_1_3513 10mio-rep3 E_20221201_NO30_400nL_HLAc1_1e7_directIP_titration_rep3_Slot2-3_1_3514.8873.1575.2 2 1 1 8.84 6.77 2.21 2.07 73.2 5 1 1 6900.0 Hydrogen 11 (A) 9 AESGAIRAL (-) 9 AESAGIALR A E/S|G/A|I R A\L 44.4 4 3 4 2 0 unbiased 30.658663 0.766231 444.2482 887.495 -0.0058 -6.5 -0.20000000000000018 6.05 1902.2 nuORFdb_human ENST00000487431.1_1_9:127624149-127624209:- ENST00000487431.1_1_9:127624149-127624209:- RPL35 ncRNA Retained Intron lncRNA GN=RPL35 ncRNA Retained Intron E_20221201_NO30_400nL_HLAc1_1e7_directIP_titration_rep3_Slot2-3_1_3514 20mio-rep1 E_20221201_NO30_400nL_HLAc1_2e7_directIP_titration_rep1_Slot2-4_1_3515.8781.1568.2 2 1 1 9.84 7.06 3.77 2.78 73.7 6 1 1 10100.0 Hydrogen 11 (A) 9 AESGAIRAL (-) 9 AESQLVLQ A E/S|G/A/I|R\A-L 77.8 7 3 5 2 1 unbiased 30.333231 0.770273 444.2478 887.495 -0.0058 -6.5 -0.2999999999999998 6.05 1902.2 nuORFdb_human ENST00000487431.1_1_9:127624149-127624209:- ENST00000487431.1_1_9:127624149-127624209:- RPL35 ncRNA Retained Intron lncRNA GN=RPL35 ncRNA Retained Intron E_20221201_NO30_400nL_HLAc1_2e7_directIP_titration_rep1_Slot2-4_1_3515 20mio-rep2 E_20221201_NO30_400nL_HLAc1_2e7_directIP_titration_rep3_Slot2-4_1_3518.8961.1554.2 2 3 1 15.22 10.48 5.46 4.74 88.1 7 1 1 24700.0 Hydrogen 11 (A) 9 AESGAIRAL (-) 9 AESAGIALR A/E|S|G/A|I|R A\L 77.8 7 5 6 4 0 dual b/y bias 30.955608 0.779527 444.248 887.495 -0.0058 -6.5 -0.20000000000000018 6.05 1902.2 nuORFdb_human ENST00000487431.1_1_9:127624149-127624209:- ENST00000487431.1_1_9:127624149-127624209:- RPL35 ncRNA Retained Intron lncRNA GN=RPL35 ncRNA Retained Intron E_20221201_NO30_400nL_HLAc1_2e7_directIP_titration_rep3_Slot2-4_1_3518 20mio-rep3 E_20221201_NO30_400nL_HLAc1_2e7_directIP_titration_rep4_Slot2-4_1_3519.8815.1565.2 2 1 1 10.12 5.93 3.0 4.18 73.4 8 1 1 6230.0 Hydrogen 11 (A) 9 AESGAIRAL (-) 9 AESAGIALR A/E/S/G/A/I\R\A\L 100.0 9 3 5 0 0 unbiased 30.453739 0.772231 444.2466 887.495 -0.0078 -8.8 -3.000000000000001 6.05 1902.2 nuORFdb_human ENST00000487431.1_1_9:127624149-127624209:- ENST00000487431.1_1_9:127624149-127624209:- RPL35 ncRNA Retained Intron lncRNA GN=RPL35 ncRNA Retained Intron E_20221201_NO30_400nL_HLAc1_2e7_directIP_titration_rep4_Slot2-4_1_3519 40mio-rep1 E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep1_Slot1-04_1_3496.9067.1545.2 2 3 1 10.55 7.68 2.92 2.87 82.4 6 1 1 22000.0 Hydrogen 11 (A) 9 AESGAIRAL (-) 9 EAQSLLRA A/E/S|G/A|I|R A L 66.7 6 3 6 3 0 dual b/y bias 31.324522 0.784946 444.2495 887.495 -0.0018 -2.0 1.2000000000000002 6.05 1902.2 nuORFdb_human ENST00000487431.1_1_9:127624149-127624209:- ENST00000487431.1_1_9:127624149-127624209:- RPL35 ncRNA Retained Intron lncRNA GN=RPL35 ncRNA Retained Intron E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep1_Slot1-04_1_3496 40mio-rep2 E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep2_Slot2-5_1_3520.8798.1510.2 2 2 1 10.65 9.54 3.3 1.11 83.7 6 1 1 20300.0 Hydrogen 11 (A) 9 AESGAIRAL (-) 9 AESAGIALR A/E/S/G/A|I|R A L 66.7 6 2 6 2 0 y-ion bias 30.395269 0.807228 444.2466 887.495 -0.0078 -8.8 -3.9000000000000004 6.05 1902.2 nuORFdb_human ENST00000487431.1_1_9:127624149-127624209:- ENST00000487431.1_1_9:127624149-127624209:- RPL35 ncRNA Retained Intron lncRNA GN=RPL35 ncRNA Retained Intron E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep2_Slot2-5_1_3520 40mio-rep3 E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep3_Slot2-5_1_3521.8782.1555.2 2 2 1 14.13 9.45 5.29 4.68 85.1 8 1 1 14200.0 Hydrogen 11 (A) 9 AESGAIRAL (-) 9 AEEAKRAI A/E/S|G/A/I|R\A\L 100.0 9 4 6 2 0 unbiased 30.338659 0.779009 444.2482 887.495 -0.0058 -6.5 -1.9000000000000004 6.05 1902.2 nuORFdb_human ENST00000487431.1_1_9:127624149-127624209:- ENST00000487431.1_1_9:127624149-127624209:- RPL35 ncRNA Retained Intron lncRNA GN=RPL35 ncRNA Retained Intron E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep3_Slot2-5_1_3521 40mio-rep4 E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep4_Slot2-5_1_3522.8803.1550.2 2 3 1 13.17 8.31 4.86 4.87 85.3 8 1 1 30500.0 Hydrogen 11 (A) 9 AESGAIRAL (-) 9 EAAGSLRAI A/E/S|G/A/I|R\A\L 100.0 9 4 6 2 0 unbiased 30.404757 0.782058 444.2476 887.495 -0.0058 -6.5 -1.7999999999999998 6.05 1902.2 nuORFdb_human ENST00000487431.1_1_9:127624149-127624209:- ENST00000487431.1_1_9:127624149-127624209:- RPL35 ncRNA Retained Intron lncRNA GN=RPL35 ncRNA Retained Intron E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep4_Slot2-5_1_3522 1mio-rep1 E_20221201_NO30_400nL_HLAc1_1e6_directIP_titration_rep1_Slot1-06_1_3498.23429.490.1 1 1 1 8.21 5.31 2.54 2.89 71.8 5 0 0 2540.0 Hydrogen 3 (M) 9 AESGLLTEL (-) 9 LQSELTEL A E/S G L|L\T\E\L 44.4 4 4 2 1 0 b-ion bias 80.910851 1.432114 932.4866 932.494 -0.0065 -7.0 -2.0999999999999996 3.8 1219.4 nuORFdb_human ENST00000369452.8_1_10:112679388-112723891:+ ENST00000369452.8_1_10:112679388-112723891:+ SHOC2 5' uORF 5' uORF GN=SHOC2 5' uORF E_20221201_NO30_400nL_HLAc1_1e6_directIP_titration_rep1_Slot1-06_1_3498 10mio-rep1 E_20221201_NO30_400nL_HLAc1_1e7_directIP_titration_rep1_Slot2-3_1_3512.23241.1543.2 2 4 1 11.42 8.52 2.07 2.9 81.6 5 0 0 40800.0 Hydrogen 3 (M) 9 AESGLLTEL (-) 9 KEAELTEI A E/S G L/L|T\E\L 44.4 4 3 3 1 0 unbiased 80.280052 0.786598 466.7465 932.494 -0.0088 -9.4 -1.7000000000000002 3.8 1219.4 nuORFdb_human ENST00000369452.8_1_10:112679388-112723891:+ ENST00000369452.8_1_10:112679388-112723891:+ SHOC2 5' uORF 5' uORF GN=SHOC2 5' uORF E_20221201_NO30_400nL_HLAc1_1e7_directIP_titration_rep1_Slot2-3_1_3512 10mio-rep2 E_20221201_NO30_400nL_HLAc1_1e7_directIP_titration_rep2_Slot2-3_1_3513.23179.505.1 1 3 1 11.33 7.53 3.01 3.8 81.2 6 0 0 38000.0 Hydrogen 3 (M) 9 AESGLLTEL (-) 9 LQSELTEL A E/S G\L|L|T\E\L 55.6 5 5 3 2 0 unbiased 80.059319 1.422966 932.4882 932.494 -0.0055 -5.9 0.7999999999999998 3.8 1219.4 nuORFdb_human ENST00000369452.8_1_10:112679388-112723891:+ ENST00000369452.8_1_10:112679388-112723891:+ SHOC2 5' uORF 5' uORF GN=SHOC2 5' uORF E_20221201_NO30_400nL_HLAc1_1e7_directIP_titration_rep2_Slot2-3_1_3513 10mio-rep3 E_20221201_NO30_400nL_HLAc1_1e7_directIP_titration_rep3_Slot2-3_1_3514.23123.1541.2 2 4 1 11.25 7.69 3.09 3.56 84.1 5 0 0 36400.0 Hydrogen 3 (M) 9 AESGLLTEL (-) 9 KEAELTEI A E/S G L/L|T\E\L 44.4 4 3 3 1 0 unbiased 79.873863 0.787667 466.7465 932.494 -0.0088 -9.4 -3.1000000000000005 3.8 1219.4 nuORFdb_human ENST00000369452.8_1_10:112679388-112723891:+ ENST00000369452.8_1_10:112679388-112723891:+ SHOC2 5' uORF 5' uORF GN=SHOC2 5' uORF E_20221201_NO30_400nL_HLAc1_1e7_directIP_titration_rep3_Slot2-3_1_3514 20mio-rep1 E_20221201_NO30_400nL_HLAc1_2e7_directIP_titration_rep1_Slot2-4_1_3515.23122.1534.2 2 8 1 11.46 8.25 3.8 3.21 81.2 6 0 0 73300.0 Hydrogen 3 (M) 9 AESGLLTEL (-) 9 LQSELTEL A E/S G/L/L|T|E\L 55.6 5 3 5 2 0 unbiased 79.847305 0.792075 466.7463 932.494 -0.0088 -9.4 -3.2 3.8 1219.4 nuORFdb_human ENST00000369452.8_1_10:112679388-112723891:+ ENST00000369452.8_1_10:112679388-112723891:+ SHOC2 5' uORF 5' uORF GN=SHOC2 5' uORF E_20221201_NO30_400nL_HLAc1_2e7_directIP_titration_rep1_Slot2-4_1_3515 20mio-rep2 E_20221201_NO30_400nL_HLAc1_2e7_directIP_titration_rep3_Slot2-4_1_3518.23165.567.1 1 6 1 12.94 8.97 3.63 3.97 84.7 5 0 0 71500.0 Hydrogen 3 (M) 9 AESGLLTEL (-) 9 KEAELTEI A E/S G L|L|T\E\L 44.4 4 4 3 2 0 unbiased 80.012665 1.386169 932.488 932.494 -0.0055 -5.9 0.39999999999999947 3.8 1219.4 nuORFdb_human ENST00000369452.8_1_10:112679388-112723891:+ ENST00000369452.8_1_10:112679388-112723891:+ SHOC2 5' uORF 5' uORF GN=SHOC2 5' uORF E_20221201_NO30_400nL_HLAc1_2e7_directIP_titration_rep3_Slot2-4_1_3518 20mio-rep3 E_20221201_NO30_400nL_HLAc1_2e7_directIP_titration_rep4_Slot2-4_1_3519.23179.498.1 1 4 1 11.1 8.57 2.36 2.53 80.7 5 0 0 75600.0 Hydrogen 3 (M) 9 AESGLLTEL (-) 9 LQSELTEL A E/S G L|L\T\E\L 44.4 4 4 2 1 0 b-ion bias 80.05233 1.427219 932.4892 932.494 -0.0045 -4.8 1.0 3.8 1219.4 nuORFdb_human ENST00000369452.8_1_10:112679388-112723891:+ ENST00000369452.8_1_10:112679388-112723891:+ SHOC2 5' uORF 5' uORF GN=SHOC2 5' uORF E_20221201_NO30_400nL_HLAc1_2e7_directIP_titration_rep4_Slot2-4_1_3519 40mio-rep1 E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep1_Slot1-04_1_3496.23355.476.1 1 1 1 10.3 7.68 2.75 2.62 77.6 5 0 0 123000.0 Hydrogen 3 (M) 9 AESGLLTEL (-) 9 LQSELTEL A E/S G L|L|T\E\L 44.4 4 4 3 2 0 unbiased 80.681747 1.440181 932.4915 932.494 -0.0015 -1.6 1.6 3.8 1219.4 nuORFdb_human ENST00000369452.8_1_10:112679388-112723891:+ ENST00000369452.8_1_10:112679388-112723891:+ SHOC2 5' uORF 5' uORF GN=SHOC2 5' uORF E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep1_Slot1-04_1_3496 40mio-rep2 E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep2_Slot2-5_1_3520.23146.494.1 1 3 1 11.87 9.24 2.85 2.63 84.9 5 0 0 86900.0 Hydrogen 3 (M) 9 AESGLLTEL (-) 9 LQSELTEL A E/S G L|L|T\E\L 44.4 4 4 3 2 0 unbiased 79.958588 1.429563 932.4906 932.494 -0.0025 -2.7 2.2 3.8 1219.4 nuORFdb_human ENST00000369452.8_1_10:112679388-112723891:+ ENST00000369452.8_1_10:112679388-112723891:+ SHOC2 5' uORF 5' uORF GN=SHOC2 5' uORF E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep2_Slot2-5_1_3520 40mio-rep3 E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep3_Slot2-5_1_3521.23157.493.1 1 2 1 8.32 5.47 3.5 2.85 72.1 5 0 0 64300.0 Hydrogen 3 (M) 9 AESGLLTEL (-) 9 LQSELTEL A E/S G L|L|T\E\L 44.4 4 4 3 2 0 unbiased 79.993935 1.430284 932.4887 932.494 -0.0045 -4.8 -0.20000000000000018 3.8 1219.4 nuORFdb_human ENST00000369452.8_1_10:112679388-112723891:+ ENST00000369452.8_1_10:112679388-112723891:+ SHOC2 5' uORF 5' uORF GN=SHOC2 5' uORF E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep3_Slot2-5_1_3521 40mio-rep4 E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep4_Slot2-5_1_3522.23091.492.1 1 1 1 7.88 6.37 1.77 1.51 70.9 5 0 0 63300.0 Hydrogen 3 (M) 9 AESGLLTEL (-) 9 LQSELTEL A E/S G L|L\T\E\L 44.4 4 4 2 1 0 b-ion bias 79.754929 1.430649 932.4892 932.494 -0.0045 -4.8 -0.09999999999999964 3.8 1219.4 nuORFdb_human ENST00000369452.8_1_10:112679388-112723891:+ ENST00000369452.8_1_10:112679388-112723891:+ SHOC2 5' uORF 5' uORF GN=SHOC2 5' uORF E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep4_Slot2-5_1_3522 5mio-rep1 E_20221201_NO30_400nL_HLAc1_5e6_directIP_titration_rep1_Slot1-37_1_3502.23423.498.1 1 1 1 9.29 7.53 1.86 1.77 75.8 5 0 0 6020.0 Hydrogen 3 (M) 9 AESGLLTEL (-) 9 LQSELTEL A E/S G L\L\T\E\L 44.4 4 4 1 0 0 b-ion bias 80.89859 1.426979 932.4844 932.494 -0.0095 -10.2 -3.1999999999999993 3.8 1219.4 nuORFdb_human ENST00000369452.8_1_10:112679388-112723891:+ ENST00000369452.8_1_10:112679388-112723891:+ SHOC2 5' uORF 5' uORF GN=SHOC2 5' uORF E_20221201_NO30_400nL_HLAc1_5e6_directIP_titration_rep1_Slot1-37_1_3502 5mio-rep2 E_20221201_NO30_400nL_HLAc1_5e6_directIP_titration_rep2_Slot2-2_1_3510.23299.512.1 1 2 1 8.98 6.65 2.32 2.34 73.8 5 0 0 12500.0 Hydrogen 3 (M) 9 AESGLLTEL (-) 9 LQSELTEL A E/S G L|L\T\E\L 44.4 4 4 2 1 0 b-ion bias 80.465408 1.419016 932.4858 932.494 -0.0075 -8.0 0.6999999999999993 3.8 1219.4 nuORFdb_human ENST00000369452.8_1_10:112679388-112723891:+ ENST00000369452.8_1_10:112679388-112723891:+ SHOC2 5' uORF 5' uORF GN=SHOC2 5' uORF E_20221201_NO30_400nL_HLAc1_5e6_directIP_titration_rep2_Slot2-2_1_3510 5mio-rep3 E_20221201_NO30_400nL_HLAc1_5e6_directIP_titration_rep3_Slot2-2_1_3511.23279.509.1 1 2 1 9.13 7.67 1.77 1.46 70.9 5 0 0 10400.0 Hydrogen 3 (M) 9 AESGLLTEL (-) 9 LQSELTEL A E/S G L|L|T\E\L 44.4 4 4 3 2 0 unbiased 80.399933 1.420702 932.484 932.494 -0.0095 -10.2 -2.0 3.8 1219.4 nuORFdb_human ENST00000369452.8_1_10:112679388-112723891:+ ENST00000369452.8_1_10:112679388-112723891:+ SHOC2 5' uORF 5' uORF GN=SHOC2 5' uORF E_20221201_NO30_400nL_HLAc1_5e6_directIP_titration_rep3_Slot2-2_1_3511 20mio-rep1 E_20221201_NO30_400nL_HLAc1_2e7_directIP_titration_rep1_Slot2-4_1_3515.9623.687.1 1 1 1 10.88 2.41 8.95 8.46 80.6 8 0 0 2960.0 Hydrogen 23 (A) 9 AESGSGPSW (R) 9 GTEGSGWPS A/E/S/G/S/G|P\S\W 100.0 9 3 6 1 0 y-ion bias 33.240036 1.314082 877.3618 877.369 -0.0066 -7.6 -1.3999999999999995 4.0 4318.6 smORF_v4|nuORFdb_human ZNRF2_7:30324548-30324682:+ ZNRF2_7:30324548-30324682:+|ENST00000323037.4_1_7:30324457-30324679:+ ZNRF2 uORF 7:30324548-30324682:+ 7 ZNRF2 5' uORF 5' uORF GN=ZNRF2 E_20221201_NO30_400nL_HLAc1_2e7_directIP_titration_rep1_Slot2-4_1_3515 20mio-rep2 E_20221201_NO30_400nL_HLAc1_2e7_directIP_titration_rep3_Slot2-4_1_3518.9810.684.1 1 1 1 11.53 5.12 8.67 6.41 78.8 7 0 0 2800.0 Hydrogen 23 (A) 9 AESGSGPSW (R) 9 GFSGPSPGDG A E/S/G/S/G|P|S\W 77.8 7 3 6 2 0 y-ion bias 33.887119 1.316147 877.3636 877.369 -0.0046 -5.3 1.0 4.0 4318.6 smORF_v4|nuORFdb_human ZNRF2_7:30324548-30324682:+ ZNRF2_7:30324548-30324682:+|ENST00000323037.4_1_7:30324457-30324679:+ ZNRF2 uORF 7:30324548-30324682:+ 7 ZNRF2 5' uORF 5' uORF GN=ZNRF2 E_20221201_NO30_400nL_HLAc1_2e7_directIP_titration_rep3_Slot2-4_1_3518 20mio-rep3 E_20221201_NO30_400nL_HLAc1_2e7_directIP_titration_rep4_Slot2-4_1_3519.9656.679.1 1 1 1 10.47 5.85 6.03 4.61 80.5 8 0 0 2900.0 Hydrogen 23 (A) 9 AESGSGPSW (R) 9 GFSGPSPGDG A/E/S/G/S/G/P\S\W 100.0 9 2 6 0 0 y-ion bias 33.35696 1.318788 877.3629 877.369 -0.0056 -6.4 -0.6000000000000005 4.0 4318.6 smORF_v4|nuORFdb_human ZNRF2_7:30324548-30324682:+ ZNRF2_7:30324548-30324682:+|ENST00000323037.4_1_7:30324457-30324679:+ ZNRF2 uORF 7:30324548-30324682:+ 7 ZNRF2 5' uORF 5' uORF GN=ZNRF2 E_20221201_NO30_400nL_HLAc1_2e7_directIP_titration_rep4_Slot2-4_1_3519 40mio-rep2 E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep2_Slot2-5_1_3520.9639.677.1 1 1 1 13.18 6.15 8.53 7.03 85.7 7 0 0 10900.0 Hydrogen 23 (A) 9 AESGSGPSW (R) 9 GFSGPSPGDG A E/S/G/S|G/P|S\W 77.8 7 3 6 2 0 y-ion bias 33.298916 1.319893 877.3644 877.369 -0.0046 -5.3 -0.39999999999999947 4.0 4318.6 smORF_v4|nuORFdb_human ZNRF2_7:30324548-30324682:+ ZNRF2_7:30324548-30324682:+|ENST00000323037.4_1_7:30324457-30324679:+ ZNRF2 uORF 7:30324548-30324682:+ 7 ZNRF2 5' uORF 5' uORF GN=ZNRF2 E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep2_Slot2-5_1_3520 40mio-rep3 E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep3_Slot2-5_1_3521.9640.676.1 1 2 1 12.29 5.55 6.37 6.74 87.3 7 0 0 9740.0 Hydrogen 23 (A) 9 AESGSGPSW (R) 9 EASGDAAAW A E/S/G/S|G|P\S\W 77.8 7 4 5 2 0 unbiased 33.304176 1.320562 877.3663 877.369 -0.0026 -3.0 1.5999999999999996 4.0 4318.6 smORF_v4|nuORFdb_human ZNRF2_7:30324548-30324682:+ ZNRF2_7:30324548-30324682:+|ENST00000323037.4_1_7:30324457-30324679:+ ZNRF2 uORF 7:30324548-30324682:+ 7 ZNRF2 5' uORF 5' uORF GN=ZNRF2 E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep3_Slot2-5_1_3521 40mio-rep4 E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep4_Slot2-5_1_3522.9671.676.1 1 2 1 7.74 2.09 6.4 5.65 72.1 6 0 0 16900.0 Hydrogen 23 (A) 9 AESGSGPSW (R) 9 GSSGFPGnPG A E/S/G/S/G|P S\W 55.6 5 2 5 1 0 y-ion bias 33.400589 1.320628 877.3638 877.369 -0.0046 -5.3 -0.5999999999999996 4.0 4318.6 smORF_v4|nuORFdb_human ZNRF2_7:30324548-30324682:+ ZNRF2_7:30324548-30324682:+|ENST00000323037.4_1_7:30324457-30324679:+ ZNRF2 uORF 7:30324548-30324682:+ 7 ZNRF2 5' uORF 5' uORF GN=ZNRF2 E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep4_Slot2-5_1_3522 20mio-rep1 E_20221201_NO30_400nL_HLAc1_2e7_directIP_titration_rep1_Slot2-4_1_3515.12697.1394.2 2 2 1 12.34 12.34 12.34 57.1 10 1 1 16900.0 Hydrogen 104 (D) 11 AESSWRAGQGW (Q) 11 A/E/S/S/W|R|A\G\Q|G\W 100.0 11 6 7 3 0 unbiased 43.855495 0.880175 617.7807 1234.56 -0.0052 -4.2 2.0 6.05 17729.6 nuORFdb_human ENST00000357304.8_1_9:134308100-134321860:+ ENST00000357304.8_1_9:134308100-134321860:+ PRRC2B Out-of-Frame Out-of-Frame GN=PRRC2B Out-of-Frame E_20221201_NO30_400nL_HLAc1_2e7_directIP_titration_rep1_Slot2-4_1_3515 20mio-rep2 E_20221201_NO30_400nL_HLAc1_2e7_directIP_titration_rep3_Slot2-4_1_3518.12843.1388.2 2 1 1 12.68 12.68 12.68 54.5 9 1 1 9220.0 Hydrogen 104 (D) 11 AESSWRAGQGW (Q) 11 A E/S|S/W|R|A\G\Q|G\W 81.8 9 7 6 4 0 dual b/y bias 44.360737 0.88409 617.781 1234.56 -0.0052 -4.2 2.0999999999999996 6.05 17729.6 nuORFdb_human ENST00000357304.8_1_9:134308100-134321860:+ ENST00000357304.8_1_9:134308100-134321860:+ PRRC2B Out-of-Frame Out-of-Frame GN=PRRC2B Out-of-Frame E_20221201_NO30_400nL_HLAc1_2e7_directIP_titration_rep3_Slot2-4_1_3518 20mio-rep3 E_20221201_NO30_400nL_HLAc1_2e7_directIP_titration_rep4_Slot2-4_1_3519.12736.1388.2 2 1 1 11.77 11.77 11.77 51.4 9 1 1 7420.0 Hydrogen 104 (D) 11 AESSWRAGQGW (Q) 11 A E/S/S|W|R|A|G\Q\G\W 81.8 9 7 6 4 0 dual b/y bias 43.994572 0.883569 617.7798 1234.56 -0.0072 -5.9 -0.10000000000000053 6.05 17729.6 nuORFdb_human ENST00000357304.8_1_9:134308100-134321860:+ ENST00000357304.8_1_9:134308100-134321860:+ PRRC2B Out-of-Frame Out-of-Frame GN=PRRC2B Out-of-Frame E_20221201_NO30_400nL_HLAc1_2e7_directIP_titration_rep4_Slot2-4_1_3519 40mio-rep1 E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep1_Slot1-04_1_3496.12973.1357.2 2 1 1 18.28 5.62 14.73 12.66 90.0 10 1 1 9970.0 Hydrogen 104 (D) 11 AESSWRAGQGW (Q) 11 AnImCPGLTSPG A/E/S/S|W|R\A|G\Q|G\W 100.0 11 7 7 4 0 dual b/y bias 44.813541 0.903569 617.782 1234.56 -0.0032 -2.6 0.6000000000000001 6.05 17729.6 nuORFdb_human ENST00000357304.8_1_9:134308100-134321860:+ ENST00000357304.8_1_9:134308100-134321860:+ PRRC2B Out-of-Frame Out-of-Frame GN=PRRC2B Out-of-Frame E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep1_Slot1-04_1_3496 40mio-rep2 E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep2_Slot2-5_1_3520.12670.1376.2 2 1 1 17.71 4.99 14.24 12.72 86.4 10 1 1 8440.0 Hydrogen 104 (D) 11 AESSWRAGQGW (Q) 11 AESSnPATLEGSA A/E/S|S/W|R\A|G/Q|G\W 100.0 11 6 8 4 0 dual b/y bias 43.768875 0.891509 617.7813 1234.56 -0.0052 -4.2 0.7000000000000002 6.05 17729.6 nuORFdb_human ENST00000357304.8_1_9:134308100-134321860:+ ENST00000357304.8_1_9:134308100-134321860:+ PRRC2B Out-of-Frame Out-of-Frame GN=PRRC2B Out-of-Frame E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep2_Slot2-5_1_3520 40mio-rep3 E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep3_Slot2-5_1_3521.12681.1382.2 2 2 1 18.36 5.14 13.87 13.22 86.5 10 1 1 20800.0 Hydrogen 104 (D) 11 AESSWRAGQGW (Q) 11 AESSnPATLEGSA A/E/S|S|W|R|A|G\Q|G\W 100.0 11 8 8 6 0 dual b/y bias 43.810627 0.887478 617.78 1234.56 -0.0072 -5.9 -1.3000000000000007 6.05 17729.6 nuORFdb_human ENST00000357304.8_1_9:134308100-134321860:+ ENST00000357304.8_1_9:134308100-134321860:+ PRRC2B Out-of-Frame Out-of-Frame GN=PRRC2B Out-of-Frame E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep3_Slot2-5_1_3521 40mio-rep4 E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep4_Slot2-5_1_3522.12677.1380.2 2 1 1 15.77 5.1 10.78 10.67 81.3 9 1 1 11200.0 Hydrogen 104 (D) 11 AESSWRAGQGW (Q) 11 EASSSEEPRnK A E/S|S/W|R\A|G\Q|G\W 81.8 9 7 6 4 0 dual b/y bias 43.777908 0.888848 617.7817 1234.56 -0.0032 -2.6 2.1 6.05 17729.6 nuORFdb_human ENST00000357304.8_1_9:134308100-134321860:+ ENST00000357304.8_1_9:134308100-134321860:+ PRRC2B Out-of-Frame Out-of-Frame GN=PRRC2B Out-of-Frame E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep4_Slot2-5_1_3522 40mio-rep1 E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep1_Slot1-04_1_3496.13933.449.1 1 1 1 11.02 4.0 7.7 7.02 84.3 7 0 0 3180.0 Hydrogen 70 (K) 10 AETGSNAVDW (-) 10 GQKDSnVGDE A E/T/G S|N\A\V\D\W 60.0 6 5 3 1 0 unbiased 48.12936 1.456561 1049.4466 1049.453 -0.0034 -3.3 -0.09999999999999964 3.66 8648.8 smORF_v4|nuORFdb_human RAB17_2:238499156-238499395:- RAB17_2:238499156-238499395:-|ENST00000392001.6_1_2:238499158-238499395:- RAB17 uORF 2:238499156-238499395:- 2 RAB17 5' uORF 5' uORF GN=RAB17 E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep1_Slot1-04_1_3496 40mio-rep2 E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep2_Slot2-5_1_3520.13630.459.1 1 2 1 8.69 2.67 5.89 6.02 79.5 6 0 0 4740.0 Hydrogen 70 (K) 10 AETGSNAVDW (-) 10 FGDnIPDRn A E/T G S/N\A\V\D\W 50.0 5 4 2 0 0 b-ion bias 47.084465 1.450138 1049.4482 1049.453 -0.0034 -3.3 1.6000000000000005 3.66 8648.8 smORF_v4|nuORFdb_human RAB17_2:238499156-238499395:- RAB17_2:238499156-238499395:-|ENST00000392001.6_1_2:238499158-238499395:- RAB17 uORF 2:238499156-238499395:- 2 RAB17 5' uORF 5' uORF GN=RAB17 E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep2_Slot2-5_1_3520 40mio-rep1 E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep1_Slot1-04_1_3496.14387.629.1 1 1 1 9.46 5.77 0.45 3.68 76.7 5 0 0 5240.0 Hydrogen 17 (T) 9 AEVAAATPL (R) 9 TVDGIGALP A E V\A\A\A|T\P L 44.4 4 5 1 1 0 b-ion bias 49.697403 1.34869 842.4568 842.462 -0.0048 -5.7 -2.5 4.0 4710.2 nuORFdb_human ENST00000561205.1_1_15:38545125-38545263:+ ENST00000561205.1_1_15:38545125-38545263:+ SPRED1 ncRNA Processed Transcript lncRNA GN=SPRED1 ncRNA Processed Transcript E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep1_Slot1-04_1_3496 40mio-rep2 E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep2_Slot2-5_1_3520.14070.639.1 1 1 1 9.1 5.54 1.83 3.56 78.5 5 0 0 3670.0 Hydrogen 17 (T) 9 AEVAAATPL (R) 9 APSSGITLP A E V\A\A\A|T\P L 44.4 4 5 1 1 0 b-ion bias 48.604027 1.342644 842.4584 842.462 -0.0038 -4.5 0.40000000000000036 4.0 4710.2 nuORFdb_human ENST00000561205.1_1_15:38545125-38545263:+ ENST00000561205.1_1_15:38545125-38545263:+ SPRED1 ncRNA Processed Transcript lncRNA GN=SPRED1 ncRNA Processed Transcript E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep2_Slot2-5_1_3520 40mio-rep4 E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep4_Slot2-5_1_3522.14065.638.1 1 1 1 9.99 4.53 2.78 5.46 74.9 5 0 0 3460.0 Hydrogen 17 (T) 9 AEVAAATPL (R) 9 SVPSATALP A E V|A\A\A|T\P L 44.4 4 5 2 2 0 b-ion bias 48.573112 1.343353 842.4568 842.462 -0.0048 -5.7 -1.0 4.0 4710.2 nuORFdb_human ENST00000561205.1_1_15:38545125-38545263:+ ENST00000561205.1_1_15:38545125-38545263:+ SPRED1 ncRNA Processed Transcript lncRNA GN=SPRED1 ncRNA Processed Transcript E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep4_Slot2-5_1_3522 20mio-rep2 E_20221201_NO30_400nL_HLAc1_2e7_directIP_titration_rep3_Slot2-4_1_3518.21176.1359.2 2 1 1 7.58 5.1 2.67 2.48 72.4 5 1 1 3850.0 Hydrogen 18 (V) 10 AEYPELRGVF (-) 10 EYAPRDGILF A E/Y|P/E L R\G V\F 30.0 3 3 3 1 0 unbiased 73.14563 0.901876 590.7982 1180.6 -0.011 -9.3 -3.000000000000001 4.53 2951.4 smORF_v4|nuORFdb_human GEMIN6_2:39005337-39005419:+ GEMIN6_2:39005337-39005419:+|ENST00000409011.5_2_2:39005336-39005417:+ GEMIN6 uORF 2:39005337-39005419:+ 2 GEMIN6 5' uORF 5' uORF GN=GEMIN6 E_20221201_NO30_400nL_HLAc1_2e7_directIP_titration_rep3_Slot2-4_1_3518 40mio-rep1 E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep1_Slot1-04_1_3496.21355.1345.2 2 1 1 7.46 5.25 3.38 2.21 65.4 5 1 1 8070.0 Hydrogen 18 (V) 10 AEYPELRGVF (-) 10 EYAPRDGILF A E/Y|P/E L R G\V\F 40.0 4 3 3 1 0 unbiased 73.770599 0.910704 590.8019 1180.6 -0.003 -2.5 0.7000000000000002 4.53 2951.4 smORF_v4|nuORFdb_human GEMIN6_2:39005337-39005419:+ GEMIN6_2:39005337-39005419:+|ENST00000409011.5_2_2:39005336-39005417:+ GEMIN6 uORF 2:39005337-39005419:+ 2 GEMIN6 5' uORF 5' uORF GN=GEMIN6 E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep1_Slot1-04_1_3496 40mio-rep2 E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep2_Slot2-5_1_3520.21142.1352.2 2 2 1 9.93 7.58 3.89 2.35 71.9 6 1 1 15700.0 Hydrogen 18 (V) 10 AEYPELRGVF (-) 10 EYAPRDGILF A E/Y|P|E L/R G\V\F 40.0 4 4 4 2 0 unbiased 73.037613 0.906448 590.798 1180.6 -0.011 -9.3 -4.4 4.53 2951.4 smORF_v4|nuORFdb_human GEMIN6_2:39005337-39005419:+ GEMIN6_2:39005337-39005419:+|ENST00000409011.5_2_2:39005336-39005417:+ GEMIN6 uORF 2:39005337-39005419:+ 2 GEMIN6 5' uORF 5' uORF GN=GEMIN6 E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep2_Slot2-5_1_3520 40mio-rep3 E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep3_Slot2-5_1_3521.21229.1340.2 2 3 1 9.1 5.36 4.26 3.74 75.6 6 1 1 13000.0 Hydrogen 18 (V) 10 AEYPELRGVF (-) 10 EAYFEQLAIP A E/Y|P/E L/R\G V\F 40.0 4 3 4 1 0 unbiased 73.334457 0.913825 590.8017 1180.6 -0.003 -2.5 2.0999999999999996 4.53 2951.4 smORF_v4|nuORFdb_human GEMIN6_2:39005337-39005419:+ GEMIN6_2:39005337-39005419:+|ENST00000409011.5_2_2:39005336-39005417:+ GEMIN6 uORF 2:39005337-39005419:+ 2 GEMIN6 5' uORF 5' uORF GN=GEMIN6 E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep3_Slot2-5_1_3521 40mio-rep4 E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep4_Slot2-5_1_3522.21175.1355.2 2 2 1 9.42 6.89 4.11 2.53 75.7 6 1 1 13500.0 Hydrogen 18 (V) 10 AEYPELRGVF (-) 10 EYAPRDGILF A E/Y|P/E L/R\G V\F 40.0 4 3 4 1 0 unbiased 73.130745 0.904327 590.7978 1180.6 -0.011 -9.3 -4.6000000000000005 4.53 2951.4 smORF_v4|nuORFdb_human GEMIN6_2:39005337-39005419:+ GEMIN6_2:39005337-39005419:+|ENST00000409011.5_2_2:39005336-39005417:+ GEMIN6 uORF 2:39005337-39005419:+ 2 GEMIN6 5' uORF 5' uORF GN=GEMIN6 E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep4_Slot2-5_1_3522 SCP E_20221201_NO30_400nL_HLAc1_4e7_PDAC_Slot2-6_1_3524.24464.423.1 1 1 1 9.63 2.01 3.54 7.62 75.8 5 0 0 4300.0 Hydrogen 12 (G) 9 AFLSGQAVF (H) 9 AGAAFGSLVF A F L\S\G Q\A\V\F 44.4 4 5 0 0 0 b-ion bias 84.516129 1.471471 939.4949 939.493 0.0016 1.7 4.1 5.57 3653.2 nuORFdb_human ENST00000523985.5_1_6:100009545-100016464:- ENST00000523985.5_1_6:100009545-100016464:-|ENST00000369217.8_1_6:100014911-100016464:-|ENST00000520371.5_1_6:100010803-100016494:- CCNC 5' Overlap uORF 5' Overlap uORF GN=CCNC 5' Overlap uORF E_20221201_NO30_400nL_HLAc1_4e7_PDAC_Slot2-6_1_3524 20mio-rep1 E_20221201_NO30_400nL_HLAc1_2e7_directIP_titration_rep1_Slot2-4_1_3515.18448.1447.2 2 1 1 10.73 7.47 2.73 3.26 86.7 7 0 0 6110.0 Hydrogen 31 (S) 9 AILGTLVHA (V) 9 IAVAHSLLA A/I/L/G|T L\V/H-A 77.8 7 2 5 1 1 y-ion bias 63.711189 0.84682 447.7703 894.541 -0.008 -8.9 -2.7 6.78 8956.2 nuORFdb_human ENST00000445399.5_1_2:70683474-70692835:- ENST00000445399.5_1_2:70683474-70692835:- TGFA Out-of-Frame Out-of-Frame GN=TGFA Out-of-Frame E_20221201_NO30_400nL_HLAc1_2e7_directIP_titration_rep1_Slot2-4_1_3515 20mio-rep3 E_20221201_NO30_400nL_HLAc1_2e7_directIP_titration_rep4_Slot2-4_1_3519.18492.1445.2 2 1 1 7.84 6.23 2.5 1.62 78.8 6 0 0 5340.0 Hydrogen 31 (S) 9 AILGTLVHA (V) 9 LALHSLGLA A I/L|G/T L/V/H-A 55.6 5 1 5 1 1 y-ion bias 63.868721 0.84824 447.771 894.541 -0.006 -6.7 -0.9000000000000004 6.78 8956.2 nuORFdb_human ENST00000445399.5_1_2:70683474-70692835:- ENST00000445399.5_1_2:70683474-70692835:- TGFA Out-of-Frame Out-of-Frame GN=TGFA Out-of-Frame E_20221201_NO30_400nL_HLAc1_2e7_directIP_titration_rep4_Slot2-4_1_3519 40mio-rep4 E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep4_Slot2-5_1_3522.18415.1439.2 2 1 1 7.75 6.2 1.66 1.54 83.1 6 0 0 3170.0 Hydrogen 31 (S) 9 AILGTLVHA (V) 9 ALLKHELA A/I/L|G/T L/V H-A 55.6 5 1 5 1 1 y-ion bias 63.597054 0.851614 447.7719 894.541 -0.004 -4.5 0.20000000000000018 6.78 8956.2 nuORFdb_human ENST00000445399.5_1_2:70683474-70692835:- ENST00000445399.5_1_2:70683474-70692835:- TGFA Out-of-Frame Out-of-Frame GN=TGFA Out-of-Frame E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep4_Slot2-5_1_3522 Eva-50mio-3fr AL20210827_EV_HLA_PDAC_PANFRO413_2D_50e6_W632_classI_fxn1.32789.32789.2 2 2 1 9.33 5.77 3.52 3.55 71.7 7 0 0 24500000.0 Hydrogen 54 (M) 9 ALAAVVTEV (A) 9 AVLAAVTEV A L\A\A\V\V-T/E/V 77.8 7 4 2 0 1 b-ion bias 0.989 82.4 58.92 100.0 0.0 436.7577 872.509 -0.0006 -0.7 -0.7999999999999999 4.0 9612.2 nuORFdb_human ENST00000441189.3_1_X:41200742-41206988:+ ENST00000441189.3_1_X:41200742-41206988:+|ENST00000399959.6_1_X:41200742-41202586:+ DDX3X 3' dORF 3' dORF GN=DDX3X 3' dORF AL20210827_EV_HLA_PDAC_PANFRO413_2D_50e6_W632_classI_fxn1 Exploris AL20221201_MKP_PDAC_01.43602.43739.1 1 2 1 9.1 6.45 2.84 2.65 69.9 6 0 0 95300000.0 Hydrogen 54 (M) 9 ALAAVVTEV (A) 9 VVAAIAISE A L\A\A\V\V|T E-V 55.6 5 5 1 1 1 b-ion bias 1.0 100.0 67.08 100.0 0.0 872.5091 872.509 0.0003 0.4 -0.4 4.0 9612.2 nuORFdb_human ENST00000441189.3_1_X:41200742-41206988:+ ENST00000441189.3_1_X:41200742-41206988:+|ENST00000399959.6_1_X:41200742-41202586:+ DDX3X 3' dORF 3' dORF GN=DDX3X 3' dORF AL20221201_MKP_PDAC_01 1mio-rep1 E_20221201_NO30_400nL_HLAc1_1e6_directIP_titration_rep1_Slot1-06_1_3498.20535.537.1 1 1 1 9.21 6.96 0.5 2.24 69.1 6 0 0 2950.0 Hydrogen 54 (M) 9 ALAAVVTEV (A) 9 VGVAVTVEV A L A\A|V|V\T\E\V 66.7 6 6 2 2 0 b-ion bias 70.918892 1.403663 872.5069 872.509 -0.0018 -2.0 2.9000000000000004 4.0 9612.2 nuORFdb_human ENST00000441189.3_1_X:41200742-41206988:+ ENST00000441189.3_1_X:41200742-41206988:+|ENST00000399959.6_1_X:41200742-41202586:+ DDX3X 3' dORF 3' dORF GN=DDX3X 3' dORF E_20221201_NO30_400nL_HLAc1_1e6_directIP_titration_rep1_Slot1-06_1_3498 1mio-rep2 E_20221201_NO30_400nL_HLAc1_1e6_directIP_titration_rep3_Slot2-1_1_3508.20487.569.1 1 1 1 7.37 5.57 1.19 1.79 61.1 6 0 0 4500.0 Hydrogen 54 (M) 9 ALAAVVTEV (A) 9 AVLAAVTEV A L A\A\V\V|T\E\V 66.7 6 6 1 1 0 b-ion bias 70.757317 1.384947 872.5031 872.509 -0.0058 -6.6 1.700000000000001 4.0 9612.2 nuORFdb_human ENST00000441189.3_1_X:41200742-41206988:+ ENST00000441189.3_1_X:41200742-41206988:+|ENST00000399959.6_1_X:41200742-41202586:+ DDX3X 3' dORF 3' dORF GN=DDX3X 3' dORF E_20221201_NO30_400nL_HLAc1_1e6_directIP_titration_rep3_Slot2-1_1_3508 10mio-rep1 E_20221201_NO30_400nL_HLAc1_1e7_directIP_titration_rep1_Slot2-3_1_3512.20296.561.1 1 3 1 11.96 9.44 1.07 2.52 75.7 6 0 0 83800.0 Hydrogen 54 (M) 9 ALAAVVTEV (A) 9 AVLAAVTEV A L A\A|V|V|T\E\V 66.7 6 6 3 3 0 dual b/y bias 70.109489 1.389794 872.5023 872.509 -0.0068 -7.7 0.0 4.0 9612.2 nuORFdb_human ENST00000441189.3_1_X:41200742-41206988:+ ENST00000441189.3_1_X:41200742-41206988:+|ENST00000399959.6_1_X:41200742-41202586:+ DDX3X 3' dORF 3' dORF GN=DDX3X 3' dORF E_20221201_NO30_400nL_HLAc1_1e7_directIP_titration_rep1_Slot2-3_1_3512 10mio-rep2 E_20221201_NO30_400nL_HLAc1_1e7_directIP_titration_rep2_Slot2-3_1_3513.20242.559.1 1 2 1 12.17 9.17 1.65 3.0 76.8 6 0 0 48100.0 Hydrogen 54 (M) 9 ALAAVVTEV (A) 9 AVLAAVTEV A L A\A|V|V|T\E\V 66.7 6 6 3 3 0 dual b/y bias 69.917603 1.390895 872.5027 872.509 -0.0058 -6.6 0.10000000000000053 4.0 9612.2 nuORFdb_human ENST00000441189.3_1_X:41200742-41206988:+ ENST00000441189.3_1_X:41200742-41206988:+|ENST00000399959.6_1_X:41200742-41202586:+ DDX3X 3' dORF 3' dORF GN=DDX3X 3' dORF E_20221201_NO30_400nL_HLAc1_1e7_directIP_titration_rep2_Slot2-3_1_3513 10mio-rep3 E_20221201_NO30_400nL_HLAc1_1e7_directIP_titration_rep3_Slot2-3_1_3514.20209.560.1 1 2 1 11.65 9.16 1.07 2.49 76.7 6 0 0 69600.0 Hydrogen 54 (M) 9 ALAAVVTEV (A) 9 AVLAAVTEV A L A\A|V|V|T\E\V 66.7 6 6 3 3 0 dual b/y bias 69.808266 1.390146 872.5034 872.509 -0.0058 -6.6 -0.2999999999999998 4.0 9612.2 nuORFdb_human ENST00000441189.3_1_X:41200742-41206988:+ ENST00000441189.3_1_X:41200742-41206988:+|ENST00000399959.6_1_X:41200742-41202586:+ DDX3X 3' dORF 3' dORF GN=DDX3X 3' dORF E_20221201_NO30_400nL_HLAc1_1e7_directIP_titration_rep3_Slot2-3_1_3514 20mio-rep1 E_20221201_NO30_400nL_HLAc1_2e7_directIP_titration_rep1_Slot2-4_1_3515.20209.557.1 1 3 1 12.87 9.08 2.9 3.79 77.7 6 0 0 172000.0 Hydrogen 54 (M) 9 ALAAVVTEV (A) 9 AVLAAVTEV A L A\A|V|V|T\E\V 66.7 6 6 3 3 0 dual b/y bias 69.790298 1.391927 872.5043 872.509 -0.0048 -5.5 0.7000000000000002 4.0 9612.2 nuORFdb_human ENST00000441189.3_1_X:41200742-41206988:+ ENST00000441189.3_1_X:41200742-41206988:+|ENST00000399959.6_1_X:41200742-41202586:+ DDX3X 3' dORF 3' dORF GN=DDX3X 3' dORF E_20221201_NO30_400nL_HLAc1_2e7_directIP_titration_rep1_Slot2-4_1_3515 20mio-rep2 E_20221201_NO30_400nL_HLAc1_2e7_directIP_titration_rep3_Slot2-4_1_3518.20242.550.1 1 2 1 12.34 8.66 1.64 3.68 73.9 6 0 0 74100.0 Hydrogen 54 (M) 9 ALAAVVTEV (A) 9 AVLAAVTEV A L A\A|V|V|T\E\V 66.7 6 6 3 3 0 dual b/y bias 69.920746 1.396402 872.504 872.509 -0.0048 -5.5 0.7999999999999998 4.0 9612.2 nuORFdb_human ENST00000441189.3_1_X:41200742-41206988:+ ENST00000441189.3_1_X:41200742-41206988:+|ENST00000399959.6_1_X:41200742-41202586:+ DDX3X 3' dORF 3' dORF GN=DDX3X 3' dORF E_20221201_NO30_400nL_HLAc1_2e7_directIP_titration_rep3_Slot2-4_1_3518 20mio-rep3 E_20221201_NO30_400nL_HLAc1_2e7_directIP_titration_rep4_Slot2-4_1_3519.20242.549.1 1 1 1 11.69 7.94 1.09 3.75 77.7 6 0 0 81500.0 Hydrogen 54 (M) 9 ALAAVVTEV (A) 9 AVLAAVTEV A L A\A|V|V\T\E\V 66.7 6 6 2 2 0 b-ion bias 69.910736 1.396594 872.5044 872.509 -0.0048 -5.5 0.2999999999999998 4.0 9612.2 nuORFdb_human ENST00000441189.3_1_X:41200742-41206988:+ ENST00000441189.3_1_X:41200742-41206988:+|ENST00000399959.6_1_X:41200742-41202586:+ DDX3X 3' dORF 3' dORF GN=DDX3X 3' dORF E_20221201_NO30_400nL_HLAc1_2e7_directIP_titration_rep4_Slot2-4_1_3519 SCP E_20221201_NO30_400nL_HLAc1_4e7_PDAC_Slot2-6_1_3524.19923.538.1 1 2 1 12.21 8.61 1.09 3.6 75.6 6 0 0 274000.0 Hydrogen 54 (M) 9 ALAAVVTEV (A) 9 AVLAAVTEV A L A\A|V|V|T\E\V 66.7 6 6 3 3 0 dual b/y bias 68.82653 1.403175 872.508 872.509 -0.0008 -0.9 1.5 4.0 9612.2 nuORFdb_human ENST00000441189.3_1_X:41200742-41206988:+ ENST00000441189.3_1_X:41200742-41206988:+|ENST00000399959.6_1_X:41200742-41202586:+ DDX3X 3' dORF 3' dORF GN=DDX3X 3' dORF E_20221201_NO30_400nL_HLAc1_4e7_PDAC_Slot2-6_1_3524 40mio-rep1 E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep1_Slot1-04_1_3496.20418.528.1 1 4 1 12.7 9.03 1.08 3.68 76.8 6 0 0 427000.0 Hydrogen 54 (M) 9 ALAAVVTEV (A) 9 AVLAAVTEV A L A\A|V|V|T\E\V 66.7 6 6 3 3 0 dual b/y bias 70.533836 1.409273 872.5083 872.509 -0.0008 -0.9 2.3000000000000003 4.0 9612.2 nuORFdb_human ENST00000441189.3_1_X:41200742-41206988:+ ENST00000441189.3_1_X:41200742-41206988:+|ENST00000399959.6_1_X:41200742-41202586:+ DDX3X 3' dORF 3' dORF GN=DDX3X 3' dORF E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep1_Slot1-04_1_3496 40mio-rep2 E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep2_Slot2-5_1_3520.20187.545.1 1 3 1 11.81 9.19 1.2 2.62 75.4 6 0 0 189000.0 Hydrogen 54 (M) 9 ALAAVVTEV (A) 9 AVLAAVTEV A L A\A|V|V|T\E\V 66.7 6 6 3 3 0 dual b/y bias 69.736412 1.399272 872.5057 872.509 -0.0028 -3.2 1.7000000000000002 4.0 9612.2 nuORFdb_human ENST00000441189.3_1_X:41200742-41206988:+ ENST00000441189.3_1_X:41200742-41206988:+|ENST00000399959.6_1_X:41200742-41202586:+ DDX3X 3' dORF 3' dORF GN=DDX3X 3' dORF E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep2_Slot2-5_1_3520 40mio-rep3 E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep3_Slot2-5_1_3521.20209.546.1 1 3 1 12.16 9.47 1.68 2.69 75.9 6 0 0 272000.0 Hydrogen 54 (M) 9 ALAAVVTEV (A) 9 AVLAAVTEV A L A\A|V|V|T\E\V 66.7 6 6 3 3 0 dual b/y bias 69.8116 1.398645 872.5062 872.509 -0.0028 -3.2 1.3999999999999995 4.0 9612.2 nuORFdb_human ENST00000441189.3_1_X:41200742-41206988:+ ENST00000441189.3_1_X:41200742-41206988:+|ENST00000399959.6_1_X:41200742-41202586:+ DDX3X 3' dORF 3' dORF GN=DDX3X 3' dORF E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep3_Slot2-5_1_3521 40mio-rep4 E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep4_Slot2-5_1_3522.20132.544.1 1 4 1 12.3 9.58 1.07 2.72 76.8 6 0 0 176000.0 Hydrogen 54 (M) 9 ALAAVVTEV (A) 9 AVLAAVTEV A L A\A|V|V|T\E\V 66.7 6 6 3 3 0 dual b/y bias 69.528717 1.400006 872.5056 872.509 -0.0028 -3.2 1.5 4.0 9612.2 nuORFdb_human ENST00000441189.3_1_X:41200742-41206988:+ ENST00000441189.3_1_X:41200742-41206988:+|ENST00000399959.6_1_X:41200742-41202586:+ DDX3X 3' dORF 3' dORF GN=DDX3X 3' dORF E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep4_Slot2-5_1_3522 5mio-rep1 E_20221201_NO30_400nL_HLAc1_5e6_directIP_titration_rep1_Slot1-37_1_3502.20514.552.1 1 2 1 11.11 7.96 1.6 3.15 75.9 6 0 0 24400.0 Hydrogen 54 (M) 9 ALAAVVTEV (A) 9 AVLAAVTEV A L A\A|V|V\T\E\V 66.7 6 6 2 2 0 b-ion bias 70.853386 1.395142 872.5008 872.509 -0.0078 -8.9 -1.9000000000000004 4.0 9612.2 nuORFdb_human ENST00000441189.3_1_X:41200742-41206988:+ ENST00000441189.3_1_X:41200742-41206988:+|ENST00000399959.6_1_X:41200742-41202586:+ DDX3X 3' dORF 3' dORF GN=DDX3X 3' dORF E_20221201_NO30_400nL_HLAc1_5e6_directIP_titration_rep1_Slot1-37_1_3502 5mio-rep2 E_20221201_NO30_400nL_HLAc1_5e6_directIP_titration_rep2_Slot2-2_1_3510.20387.564.1 1 1 1 11.48 8.43 1.56 3.05 73.5 7 0 0 6240.0 Hydrogen 54 (M) 9 ALAAVVTEV (A) 9 VGVAVTVEV A L/A\A|V|V\T\E\V 77.8 7 6 3 2 0 b-ion bias 70.412735 1.387581 872.5029 872.509 -0.0058 -6.6 2.0999999999999996 4.0 9612.2 nuORFdb_human ENST00000441189.3_1_X:41200742-41206988:+ ENST00000441189.3_1_X:41200742-41206988:+|ENST00000399959.6_1_X:41200742-41202586:+ DDX3X 3' dORF 3' dORF GN=DDX3X 3' dORF E_20221201_NO30_400nL_HLAc1_5e6_directIP_titration_rep2_Slot2-2_1_3510 5mio-rep3 E_20221201_NO30_400nL_HLAc1_5e6_directIP_titration_rep3_Slot2-2_1_3511.20371.564.1 1 2 1 9.71 6.74 1.05 2.97 74.3 6 0 0 22100.0 Hydrogen 54 (M) 9 ALAAVVTEV (A) 9 AVLAAVTEV A L A\A|V\V\T\E\V 66.7 6 6 1 1 0 b-ion bias 70.358528 1.387751 872.5026 872.509 -0.0058 -6.6 1.5999999999999996 4.0 9612.2 nuORFdb_human ENST00000441189.3_1_X:41200742-41206988:+ ENST00000441189.3_1_X:41200742-41206988:+|ENST00000399959.6_1_X:41200742-41202586:+ DDX3X 3' dORF 3' dORF GN=DDX3X 3' dORF E_20221201_NO30_400nL_HLAc1_5e6_directIP_titration_rep3_Slot2-2_1_3511 Eva-50mio-3fr AL20210827_EV_HLA_PDAC_PANFRO413_2D_50e6_W632_classI_fxn2.33857.33857.2 2 1 1 11.66 4.36 6.33 7.29 63.4 7 0 0 4680000.0 Hydrogen 102 (P) 9 ALAEVFHQL (H) 9 IAAnGVFHVV A L|A|E/V/F/H/Q-L 77.8 7 2 6 2 1 y-ion bias 0.978 56.5 62.15 100.0 0.0 514.2817 1027.557 -0.0009 -0.9 -1.2 5.24 14110.9 smORF_v4|nuORFdb_human RP11-274B21.1_7:128173707-128231913:+ RP11-274B21.1_7:128173707-128231913:+|ENST00000605862.4_1_7:128214806-128231910:+|ENST00000479267.2_3_7:128218036-128231910:+ RP11-274B21.1 lncRNA 7:128173707-128231913:+ 7 RP11-274B21.14 ncRNA Processed Transcript lncRNA GN=RP11-274B21.1 AL20210827_EV_HLA_PDAC_PANFRO413_2D_50e6_W632_classI_fxn2 SCP E_20221201_NO30_400nL_HLAc1_4e7_PDAC_Slot2-6_1_3524.3676.1683.3 3 2 1 10.32 5.9 6.86 4.42 76.0 6 2 3 42400.0 Hydrogen 2 (M) 9 ALARKLPRC (-) 9 ALKARPLRC A/L/A/R K|L|P R\C 55.6 5 3 5 2 0 unbiased Carbamidomethylation C:Carbamidomethylation, 12.712 0.697768 362.2164 1084.641 -0.0073 -6.8 -4.4 12.01 1215.5 nuORFdb_human ENST00000528641.6_2_11:67250523-67250553:+ ENST00000528641.6_2_11:67250523-67250553:+ AIP 5' uORF 5' uORF GN=AIP 5' uORF E_20221201_NO30_400nL_HLAc1_4e7_PDAC_Slot2-6_1_3524 Exploris_FAIMS VH20221207_MKP_FAIMS2022E_HLAc1_4e7_PDAC.13186.13186.3 3 2 1 8.63 3.81 3.8 4.82 60.2 7 2 3 19000000.0 Hydrogen 2 (M) 9 ALARKLPRC (-) 9 ALKARPLRC A/L|A/R/K|L|P R-C 77.8 7 3 6 3 1 dual b/y bias Carbamidomethylation C:Carbamidomethylation, 0.987 74.7 28.6 53.47 -50.0 362.2175 1084.641 -0.0029 -2.7 -0.10000000000000009 12.01 1215.5 nuORFdb_human ENST00000528641.6_2_11:67250523-67250553:+ ENST00000528641.6_2_11:67250523-67250553:+ AIP 5' uORF 5' uORF GN=AIP 5' uORF VH20221207_MKP_FAIMS2022E_HLAc1_4e7_PDAC 40mio-rep2 E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep2_Slot2-5_1_3520.24410.464.1 1 2 1 10.77 8.41 2.11 2.37 73.5 6 0 0 7940.0 Hydrogen 47 (E) 9 ALIEVVEVA (A) 9 VnLVELALA A L I\E\V\V\E\V\A 66.7 6 6 0 0 0 b-ion bias 84.324089 1.447381 942.5446 942.551 -0.0056 -6.0 -1.0999999999999996 3.8 11277.2 nuORFdb_human ENST00000226796.6_1_4:110736862-110737531:+ ENST00000226796.6_1_4:110736862-110737531:+ GAR1 5' Overlap uORF 5' Overlap uORF GN=GAR1 5' Overlap uORF E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep2_Slot2-5_1_3520 40mio-rep4 E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep4_Slot2-5_1_3522.24419.465.1 1 1 1 11.37 7.45 2.51 3.92 75.9 6 0 0 3690.0 Hydrogen 47 (E) 9 ALIEVVEVA (A) 9 VnLVELALA A L I\E\V\V\E\V\A 66.7 6 6 0 0 0 b-ion bias 84.341377 1.446954 942.5458 942.551 -0.0046 -4.9 -0.20000000000000018 3.8 11277.2 nuORFdb_human ENST00000226796.6_1_4:110736862-110737531:+ ENST00000226796.6_1_4:110736862-110737531:+ GAR1 5' Overlap uORF 5' Overlap uORF GN=GAR1 5' Overlap uORF E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep4_Slot2-5_1_3522 5mio-rep1 E_20221201_NO30_400nL_HLAc1_5e6_directIP_titration_rep1_Slot1-37_1_3502.21201.554.1 1 1 1 7.12 5.74 1.7 1.39 63.6 6 0 0 7790.0 Hydrogen 7 (T) 9 ALISVVTTA (S) 9 TVGLSLLTA A L I\S\V\V\T\T\A 66.7 6 6 0 0 0 b-ion bias 73.22496 1.3938 874.5176 874.524 -0.0064 -7.3 -0.2999999999999998 5.57 2277.4 nuORFdb_human ENST00000616174.1_1_3:113442939-113464857:- ENST00000616174.1_1_3:113442939-113464857:-|ENST00000493900.5_1_3:113442900-113464857:- NAA50 5' Overlap uORF 5' Overlap uORF GN=NAA50 5' Overlap uORF E_20221201_NO30_400nL_HLAc1_5e6_directIP_titration_rep1_Slot1-37_1_3502 5mio-rep2 E_20221201_NO30_400nL_HLAc1_5e6_directIP_titration_rep2_Slot2-2_1_3510.21074.572.1 1 1 1 8.03 4.4 2.05 3.64 70.6 6 0 0 3030.0 Hydrogen 7 (T) 9 ALISVVTTA (S) 9 SAVLIVTTA A L I\S|V\V\T\T\A 66.7 6 6 1 1 0 b-ion bias 72.783958 1.383197 874.5157 874.524 -0.0084 -9.6 -0.9000000000000004 5.57 2277.4 nuORFdb_human ENST00000616174.1_1_3:113442939-113464857:- ENST00000616174.1_1_3:113442939-113464857:-|ENST00000493900.5_1_3:113442900-113464857:- NAA50 5' Overlap uORF 5' Overlap uORF GN=NAA50 5' Overlap uORF E_20221201_NO30_400nL_HLAc1_5e6_directIP_titration_rep2_Slot2-2_1_3510 40mio-rep1 E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep1_Slot1-04_1_3496.27773.1355.2 2 1 1 12.84 10.02 0.55 2.81 85.2 6 0 0 5710.0 Hydrogen 36 (H) 11 ALLSSLFPAAN (-) 11 ALLSSYSIHL A/L/L|S|S|L|F P A A N 54.5 6 4 6 4 0 dual b/y bias 95.947777 0.904653 552.3066 1103.61 -0.0028 -2.5 0.7000000000000002 5.57 5289.2 nuORFdb_human T036117_10:4808890-4809028:- T036117_10:4808890-4809028:- lincRNA lncRNA GN=nan lincRNA E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep1_Slot1-04_1_3496 20mio-rep3 E_20221201_NO30_400nL_HLAc1_2e7_directIP_titration_rep4_Slot2-4_1_3519.19911.621.1 1 3 1 7.58 5.47 3.79 2.11 74.1 8 0 0 3950.0 Hydrogen 16 (P) 9 ALPEPLAAA (L) 9 ALPLPEAAA A/L/P\E/P\L-A\A\A 100.0 9 4 3 0 1 unbiased 68.768295 1.353479 852.4755 852.482 -0.0065 -7.7 -1.9000000000000004 4.0 7935.9 nuORFdb_human ENST00000371564.7_1_20:50139799-50140015:- ENST00000371564.7_1_20:50139799-50140015:- NFATC2 Out-of-Frame Out-of-Frame GN=NFATC2 Out-of-Frame E_20221201_NO30_400nL_HLAc1_2e7_directIP_titration_rep4_Slot2-4_1_3519 40mio-rep1 E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep1_Slot1-04_1_3496.20091.597.1 1 1 1 11.42 7.88 7.41 3.54 86.4 7 0 0 4870.0 Hydrogen 16 (P) 9 ALPEPLAAA (L) 9 ALPLPEAAA A L/P\E|P\L\A\A\A 77.8 7 6 2 1 0 b-ion bias 69.4048 1.367829 852.4805 852.482 -0.0025 -3.0 0.20000000000000018 4.0 7935.9 nuORFdb_human ENST00000371564.7_1_20:50139799-50140015:- ENST00000371564.7_1_20:50139799-50140015:- NFATC2 Out-of-Frame Out-of-Frame GN=NFATC2 Out-of-Frame E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep1_Slot1-04_1_3496 40mio-rep2 E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep2_Slot2-5_1_3520.19809.615.1 1 2 1 11.72 7.73 7.0 3.99 88.9 7 0 0 6530.0 Hydrogen 16 (P) 9 ALPEPLAAA (L) 9 ALPLPEAAA A L/P\E|P\L\A\A\A 77.8 7 6 2 1 0 b-ion bias 68.430702 1.357511 852.4792 852.482 -0.0035 -4.2 0.7000000000000002 4.0 7935.9 nuORFdb_human ENST00000371564.7_1_20:50139799-50140015:- ENST00000371564.7_1_20:50139799-50140015:- NFATC2 Out-of-Frame Out-of-Frame GN=NFATC2 Out-of-Frame E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep2_Slot2-5_1_3520 40mio-rep3 E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep3_Slot2-5_1_3521.19823.613.1 1 2 1 7.79 4.96 2.46 2.83 77.0 7 0 0 5840.0 Hydrogen 16 (P) 9 ALPEPLAAA (L) 9 ALPLPEAAA A L/P\E/P\L-A\A\A 77.8 7 4 2 0 1 b-ion bias 68.478226 1.358249 852.4761 852.482 -0.0065 -7.7 -3.1000000000000005 4.0 7935.9 nuORFdb_human ENST00000371564.7_1_20:50139799-50140015:- ENST00000371564.7_1_20:50139799-50140015:- NFATC2 Out-of-Frame Out-of-Frame GN=NFATC2 Out-of-Frame E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep3_Slot2-5_1_3521 10mio-rep2 E_20221201_NO30_400nL_HLAc1_1e7_directIP_titration_rep2_Slot2-3_1_3513.10715.1505.2 2 1 1 7.86 5.83 2.79 2.03 77.4 6 1 1 3100.0 Hydrogen 19 (L) 9 ALPEVQKQV (-) 9 LAPEVEVRV A L/P/E/V Q/K\Q\V 55.6 5 2 4 0 0 y-ion bias 37.019432 0.810106 506.2903 1011.583 -0.0106 -10.5 -3.8 6.05 2869.4 nuORFdb_human ENST00000405489.7_2_2:27435269-27435350:+ ENST00000405489.7_2_2:27435269-27435350:+ ATRAID 5' Overlap uORF 5' Overlap uORF GN=ATRAID 5' Overlap uORF E_20221201_NO30_400nL_HLAc1_1e7_directIP_titration_rep2_Slot2-3_1_3513 20mio-rep1 E_20221201_NO30_400nL_HLAc1_2e7_directIP_titration_rep1_Slot2-4_1_3515.10630.1502.2 2 1 1 9.03 6.46 2.07 2.56 86.2 7 1 1 6220.0 Hydrogen 19 (L) 9 ALPEVQKQV (-) 9 LAPEVEVRV A/L/P/E/V Q/K-Q\V 77.8 7 1 5 0 1 y-ion bias 36.71627 0.812245 506.2934 1011.583 -0.0046 -4.5 1.7000000000000002 6.05 2869.4 nuORFdb_human ENST00000405489.7_2_2:27435269-27435350:+ ENST00000405489.7_2_2:27435269-27435350:+ ATRAID 5' Overlap uORF 5' Overlap uORF GN=ATRAID 5' Overlap uORF E_20221201_NO30_400nL_HLAc1_2e7_directIP_titration_rep1_Slot2-4_1_3515 20mio-rep2 E_20221201_NO30_400nL_HLAc1_2e7_directIP_titration_rep3_Slot2-4_1_3518.10768.1498.2 2 1 1 8.55 5.95 2.11 2.6 83.8 7 1 1 4940.0 Hydrogen 19 (L) 9 ALPEVQKQV (-) 9 LAPEVEVRV A L/P/E/V-Q\K\Q\V 77.8 7 3 3 0 1 unbiased 37.194542 0.814812 506.2934 1011.583 -0.0046 -4.5 1.7999999999999998 6.05 2869.4 nuORFdb_human ENST00000405489.7_2_2:27435269-27435350:+ ENST00000405489.7_2_2:27435269-27435350:+ ATRAID 5' Overlap uORF 5' Overlap uORF GN=ATRAID 5' Overlap uORF E_20221201_NO30_400nL_HLAc1_2e7_directIP_titration_rep3_Slot2-4_1_3518 20mio-rep3 E_20221201_NO30_400nL_HLAc1_2e7_directIP_titration_rep4_Slot2-4_1_3519.10675.1501.2 2 1 1 7.65 6.07 2.56 1.58 81.0 6 1 1 5240.0 Hydrogen 19 (L) 9 ALPEVQKQV (-) 9 LAPEVEVRV A L/P/E/V\Q K\Q\V 55.6 5 3 3 0 0 unbiased 36.878437 0.813166 506.2915 1011.583 -0.0066 -6.5 -0.7000000000000002 6.05 2869.4 nuORFdb_human ENST00000405489.7_2_2:27435269-27435350:+ ENST00000405489.7_2_2:27435269-27435350:+ ATRAID 5' Overlap uORF 5' Overlap uORF GN=ATRAID 5' Overlap uORF E_20221201_NO30_400nL_HLAc1_2e7_directIP_titration_rep4_Slot2-4_1_3519 40mio-rep1 E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep1_Slot1-04_1_3496.10944.1473.2 2 2 1 15.36 11.25 5.63 4.1 92.0 8 1 1 13000.0 Hydrogen 19 (L) 9 ALPEVQKQV (-) 9 LAPEVEVRV A/L/P/E|V\Q/K\Q\V 100.0 9 4 5 1 0 unbiased 37.806053 0.830351 506.2936 1011.583 -0.0026 -2.6 0.6000000000000001 6.05 2869.4 nuORFdb_human ENST00000405489.7_2_2:27435269-27435350:+ ENST00000405489.7_2_2:27435269-27435350:+ ATRAID 5' Overlap uORF 5' Overlap uORF GN=ATRAID 5' Overlap uORF E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep1_Slot1-04_1_3496 40mio-rep2 E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep2_Slot2-5_1_3520.10643.1492.2 2 1 1 12.04 9.49 2.82 2.55 88.0 7 1 1 9000.0 Hydrogen 19 (L) 9 ALPEVQKQV (-) 9 LAPEVEVRV A L/P/E|V-Q|K-Q\V 77.8 7 3 4 2 2 unbiased 36.767349 0.818495 506.2923 1011.583 -0.0066 -6.5 -1.5999999999999996 6.05 2869.4 nuORFdb_human ENST00000405489.7_2_2:27435269-27435350:+ ENST00000405489.7_2_2:27435269-27435350:+ ATRAID 5' Overlap uORF 5' Overlap uORF GN=ATRAID 5' Overlap uORF E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep2_Slot2-5_1_3520 40mio-rep3 E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep3_Slot2-5_1_3521.10647.1490.2 2 1 1 12.08 7.92 3.32 4.16 90.1 7 1 1 6390.0 Hydrogen 19 (L) 9 ALPEVQKQV (-) 9 AIPENLLKN A/L/P/E/V Q|K\Q\V 77.8 7 3 5 1 0 unbiased 36.783707 0.82013 506.2907 1011.583 -0.0086 -8.5 -3.9000000000000004 6.05 2869.4 nuORFdb_human ENST00000405489.7_2_2:27435269-27435350:+ ENST00000405489.7_2_2:27435269-27435350:+ ATRAID 5' Overlap uORF 5' Overlap uORF GN=ATRAID 5' Overlap uORF E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep3_Slot2-5_1_3521 40mio-rep4 E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep4_Slot2-5_1_3522.10653.1486.2 2 1 1 11.71 8.63 4.1 3.08 91.9 8 1 1 7350.0 Hydrogen 19 (L) 9 ALPEVQKQV (-) 9 LAPEVEVRV A/L/P/E/V\Q/K\Q\V 100.0 9 3 5 0 0 unbiased 36.790295 0.822097 506.2916 1011.583 -0.0066 -6.5 -1.7999999999999998 6.05 2869.4 nuORFdb_human ENST00000405489.7_2_2:27435269-27435350:+ ENST00000405489.7_2_2:27435269-27435350:+ ATRAID 5' Overlap uORF 5' Overlap uORF GN=ATRAID 5' Overlap uORF E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep4_Slot2-5_1_3522 SCP E_20221201_NO30_400nL_HLAc1_4e7_PDAC_Slot2-6_1_3524.29459.694.1 1 1 1 7.85 6.25 1.6 1.6 70.1 7 0 0 6900.0 Hydrogen 15 (L) 8 ALPPIPSP (Q) 8 ALPPPSPL A/L/P/P|I\P-S-P 100.0 8 2 4 1 2 y-ion bias 101.779839 1.309671 791.4626 791.466 -0.0032 -4.0 -1.6 5.57 7362.5 nuORFdb_human ENST00000640999.1_1_17:61818885-61819092:- ENST00000640999.1_1_17:61818885-61819092:- STRADA 5' uORF 5' uORF GN=STRADA 5' uORF E_20221201_NO30_400nL_HLAc1_4e7_PDAC_Slot2-6_1_3524 Exploris AL20221201_MKP_PDAC_01.57732.57732.2 2 2 1 11.65 9.43 3.95 2.22 78.5 6 0 0 7410000.0 Hydrogen 109 (A) 9 ALSWFLNPI (T) 9 VATALRnMAL A/L/S/W F\L\N P|I 55.6 5 3 4 1 0 unbiased -0.063 74.8 87.98 100.0 0.0 530.7933 1060.583 -0.0033 -3.1 -3.9000000000000004 5.57 13394.9 nuORFdb_human ENST00000609274.5_2_19:15562735-15563116:- ENST00000609274.5_2_19:15562735-15563116:- RASAL3 3' Overlap dORF 3' Overlap dORF GN=RASAL3 3' Overlap dORF AL20221201_MKP_PDAC_01 20mio-rep1 E_20221201_NO30_400nL_HLAc1_2e7_directIP_titration_rep1_Slot2-4_1_3515.13883.1546.2 2 1 1 8.66 7.11 2.18 1.55 86.5 6 0 0 3400.0 Hydrogen 27 (M) 9 ALVSGLHGV (H) 9 ALVSHVLN A/L/V/S/G L/H-G V 55.6 5 0 5 0 1 y-ion bias 47.950306 0.784441 426.7471 852.494 -0.0071 -8.3 -2.1000000000000005 6.78 5920.8 nuORFdb_human ENST00000572179.5_1_17:3623679-3627129:- ENST00000572179.5_1_17:3623679-3627129:-|ENST00000570360.1_1_17:3626633-3627135:- ITGAE ncRNA Processed Transcript lncRNA GN=ITGAE ncRNA Processed Transcript E_20221201_NO30_400nL_HLAc1_2e7_directIP_titration_rep1_Slot2-4_1_3515 20mio-rep2 E_20221201_NO30_400nL_HLAc1_2e7_directIP_titration_rep3_Slot2-4_1_3518.28430.374.1 1 1 1 7.2 2.69 3.08 4.51 67.1 6 0 0 4310.0 Hydrogen 8 (Q) 9 ALWELSAAL (L) 9 LASAQGATAAI A L W|E\L\S\A\A\L 66.7 6 6 1 1 0 b-ion bias 98.194092 1.500972 973.525 973.535 -0.0103 -10.6 -4.3 4.0 2379.8 nuORFdb_human ENST00000571146.1_3_17:7160220-7160283:+ ENST00000571146.1_3_17:7160220-7160283:+ ELP5 3' dORF 3' dORF GN=ELP5 3' dORF E_20221201_NO30_400nL_HLAc1_2e7_directIP_titration_rep3_Slot2-4_1_3518 20mio-rep3 E_20221201_NO30_400nL_HLAc1_2e7_directIP_titration_rep4_Slot2-4_1_3519.28440.370.1 1 1 1 7.48 3.82 3.67 3.66 68.4 6 0 0 5090.0 Hydrogen 8 (Q) 9 ALWELSAAL (L) 9 AASLAWELL A L W\E\L\S\A\A\L 66.7 6 6 0 0 0 b-ion bias 98.225777 1.503051 973.5286 973.535 -0.0063 -6.5 -0.7000000000000002 4.0 2379.8 nuORFdb_human ENST00000571146.1_3_17:7160220-7160283:+ ENST00000571146.1_3_17:7160220-7160283:+ ELP5 3' dORF 3' dORF GN=ELP5 3' dORF E_20221201_NO30_400nL_HLAc1_2e7_directIP_titration_rep4_Slot2-4_1_3519 40mio-rep1 E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep1_Slot1-04_1_3496.28515.347.1 1 1 1 7.67 4.63 2.57 3.05 69.2 6 0 0 28400.0 Hydrogen 8 (Q) 9 ALWELSAAL (L) 9 ALGGTVNASAL A L W\E\L\S\A\A\L 66.7 6 6 0 0 0 b-ion bias 98.511215 1.516552 973.5317 973.535 -0.0033 -3.4 -0.19999999999999973 4.0 2379.8 nuORFdb_human ENST00000571146.1_3_17:7160220-7160283:+ ENST00000571146.1_3_17:7160220-7160283:+ ELP5 3' dORF 3' dORF GN=ELP5 3' dORF E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep1_Slot1-04_1_3496 40mio-rep2 E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep2_Slot2-5_1_3520.28438.364.1 1 1 1 8.88 6.11 3.69 2.77 75.7 6 0 0 20800.0 Hydrogen 8 (Q) 9 ALWELSAAL (L) 9 AASLAWELL A L W\E\L\S\A\A\L 66.7 6 6 0 0 0 b-ion bias 98.240685 1.506372 973.5317 973.535 -0.0033 -3.4 1.5000000000000004 4.0 2379.8 nuORFdb_human ENST00000571146.1_3_17:7160220-7160283:+ ENST00000571146.1_3_17:7160220-7160283:+ ELP5 3' dORF 3' dORF GN=ELP5 3' dORF E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep2_Slot2-5_1_3520 40mio-rep3 E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep3_Slot2-5_1_3521.28437.364.1 1 1 1 8.14 5.3 2.28 2.84 68.6 6 0 0 21300.0 Hydrogen 8 (Q) 9 ALWELSAAL (L) 9 AASLAWELL A L W\E\L\S\A\A\L 66.7 6 6 0 0 0 b-ion bias 98.230003 1.506537 973.5304 973.535 -0.0053 -5.5 -0.9000000000000004 4.0 2379.8 nuORFdb_human ENST00000571146.1_3_17:7160220-7160283:+ ENST00000571146.1_3_17:7160220-7160283:+ ELP5 3' dORF 3' dORF GN=ELP5 3' dORF E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep3_Slot2-5_1_3521 20mio-rep1 E_20221201_NO30_400nL_HLAc1_2e7_directIP_titration_rep1_Slot2-4_1_3515.21957.563.1 1 1 1 7.01 2.03 2.62 4.98 59.1 7 0 0 2060.0 Hydrogen 16 (P) 9 ALWSGSLTA (-) 9 LSWASGITA A L/W|S/G\S\L\T\A 77.8 7 5 3 1 0 unbiased 75.824661 1.388331 905.4698 905.473 -0.0027 -3.0 3.2 5.57 2543.0 nuORFdb_human ENST00000546701.5_1_12:49062937-49063012:- ENST00000546701.5_1_12:49062937-49063012:- KANSL2 3' dORF 3' dORF GN=KANSL2 3' dORF E_20221201_NO30_400nL_HLAc1_2e7_directIP_titration_rep1_Slot2-4_1_3515 20mio-rep1 E_20221201_NO30_400nL_HLAc1_2e7_directIP_titration_rep1_Slot2-4_1_3515.18545.445.1 1 2 1 8.65 2.91 6.54 5.73 54.9 5 0 0 3550.0 M5m M6m Hydrogen 4 (S) 9 AmmTSGFLV (-) 9 mnATnHAIV A m m|T\S\G F\L\V 44.4 4 5 1 1 0 b-ion bias m:Oxidized methionine 64.045998 1.45875 988.4437 956.458 31.986 -3.9 2.3000000000000003 5.57 1275.5 nuORFdb_human ENST00000547170.1_1_12:109094997-109095036:- ENST00000547170.1_1_12:109094997-109095036:- CORO1C ncRNA Retained Intron lncRNA GN=CORO1C ncRNA Retained Intron E_20221201_NO30_400nL_HLAc1_2e7_directIP_titration_rep1_Slot2-4_1_3515 20mio-rep2 E_20221201_NO30_400nL_HLAc1_2e7_directIP_titration_rep3_Slot2-4_1_3518.18634.435.1 1 2 1 10.2 2.52 7.68 7.68 60.5 7 0 0 4070.0 M5m M6m Hydrogen 4 (S) 9 AmmTSGFLV (-) 9 LFGSmFGnV A m/m\T\S\G\F\L\V 77.8 7 6 1 0 0 b-ion bias m:Oxidized methionine 64.363312 1.464853 988.4453 956.458 31.987 -2.8 3.5 5.57 1275.5 nuORFdb_human ENST00000547170.1_1_12:109094997-109095036:- ENST00000547170.1_1_12:109094997-109095036:- CORO1C ncRNA Retained Intron lncRNA GN=CORO1C ncRNA Retained Intron E_20221201_NO30_400nL_HLAc1_2e7_directIP_titration_rep3_Slot2-4_1_3518 40mio-rep1 E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep1_Slot1-04_1_3496.18842.421.1 1 2 1 11.77 2.5 9.18 9.28 68.7 7 0 0 9880.0 M5m M6m Hydrogen 4 (S) 9 AmmTSGFLV (-) 9 HmAATnnLV A m/m|T\S\G\F\L\V 77.8 7 6 2 1 0 b-ion bias m:Oxidized methionine 65.089783 1.472884 988.4447 956.458 31.987 -2.8 0.40000000000000036 5.57 1275.5 nuORFdb_human ENST00000547170.1_1_12:109094997-109095036:- ENST00000547170.1_1_12:109094997-109095036:- CORO1C ncRNA Retained Intron lncRNA GN=CORO1C ncRNA Retained Intron E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep1_Slot1-04_1_3496 40mio-rep2 E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep2_Slot2-5_1_3520.18489.437.1 1 1 1 11.07 3.26 7.81 7.81 56.4 6 0 0 3680.0 M5m M6m Hydrogen 4 (S) 9 AmmTSGFLV (-) 9 LFGSmFGnV A m/m|T\S G\F\L\V 55.6 5 5 2 1 0 b-ion bias m:Oxidized methionine 63.870945 1.46319 988.4451 956.458 31.987 -2.8 2.1000000000000005 5.57 1275.5 nuORFdb_human ENST00000547170.1_1_12:109094997-109095036:- ENST00000547170.1_1_12:109094997-109095036:- CORO1C ncRNA Retained Intron lncRNA GN=CORO1C ncRNA Retained Intron E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep2_Slot2-5_1_3520 40mio-rep3 E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep3_Slot2-5_1_3521.18523.434.1 1 1 1 10.76 3.93 6.61 6.82 61.4 7 0 0 2990.0 M5m M6m Hydrogen 4 (S) 9 AmmTSGFLV (-) 9 LFGSmFGnV A m/m\T\S\G\F\L\V 77.8 7 6 1 0 0 b-ion bias m:Oxidized methionine 63.986866 1.465048 988.445 956.458 31.987 -2.8 1.7999999999999998 5.57 1275.5 nuORFdb_human ENST00000547170.1_1_12:109094997-109095036:- ENST00000547170.1_1_12:109094997-109095036:- CORO1C ncRNA Retained Intron lncRNA GN=CORO1C ncRNA Retained Intron E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep3_Slot2-5_1_3521 40mio-rep4 E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep4_Slot2-5_1_3522.18519.431.1 1 1 1 9.27 2.35 6.92 6.92 56.5 7 0 0 5270.0 M5m M6m Hydrogen 4 (S) 9 AmmTSGFLV (-) 9 LFGSmFGnV A m/m\T\S\G\F\L\V 77.8 7 6 1 0 0 b-ion bias m:Oxidized methionine 63.956059 1.466755 988.4422 956.458 31.984 -5.9 -1.2000000000000002 5.57 1275.5 nuORFdb_human ENST00000547170.1_1_12:109094997-109095036:- ENST00000547170.1_1_12:109094997-109095036:- CORO1C ncRNA Retained Intron lncRNA GN=CORO1C ncRNA Retained Intron E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep4_Slot2-5_1_3522 SCP E_20221201_NO30_400nL_HLAc1_4e7_PDAC_Slot2-6_1_3524.9525.857.1 1 1 1 10.97 8.85 2.03 2.11 86.2 7 0 0 4430.0 Hydrogen 13 (A) 9 APAAAGAAL (H) 9 AAAPAAGAI A/P A/A|A\G\A\A\L 77.8 7 5 3 1 0 unbiased 32.907814 1.211296 712.3958 712.399 -0.0028 -4.0 -1.6 5.57 22186.6 nuORFdb_human ENST00000461415.5_1_3:183962900-183966669:- ENST00000461415.5_1_3:183962900-183966669:-|ENST00000414845.5_1_3:183961725-183966669:-|ENST00000397676.7_1_3:183963587-183966660:- ALG3 3' Overlap dORF 3' Overlap dORF GN=ALG3 3' Overlap dORF E_20221201_NO30_400nL_HLAc1_4e7_PDAC_Slot2-6_1_3524 Exploris_FAIMS VH20221207_MKP_FAIMS2022E_HLAc1_4e7_PDAC.27104.27104.2 2 1 1 12.57 10.24 1.2 2.33 84.0 6 1 1 6420000.0 Hydrogen 49 (A) 8 APAARCLL (R) 8 ACRIPAAL A/P|A/A/R\C L|L 75.0 6 3 5 2 0 unbiased Carbamidomethylation C:Carbamidomethylation, 0.997 97.1 54.45 57.97 -70.0 436.2434 871.482 -0.0024 -2.8 -0.19999999999999973 9.79 14222.0 nuORFdb_human ENST00000513185.1_1_5:98109449-98109824:+ ENST00000513185.1_1_5:98109449-98109824:+ RGMB 5' Overlap uORF 5' Overlap uORF GN=RGMB 5' Overlap uORF VH20221207_MKP_FAIMS2022E_HLAc1_4e7_PDAC Exploris_FAIMS VH20221207_MKP_FAIMS2022E_HLAc1_4e7_PDAC.37235.37355.2 2 1 1 13.17 4.84 7.13 8.33 81.8 8 1 1 28700000.0 Hydrogen 28 (M) 9 APACAKLVL (L) 9 APFLPWVL A/P|A/C\A-K\L|V/L 100.0 9 4 5 2 1 unbiased Carbamidomethylation C:Carbamidomethylation, 0.999 100.0 69.52 13.86 -70.0 471.7743 942.544 -0.0027 -2.9 -0.2999999999999998 8.8 9092.4 nuORFdb_human ENST00000570382.1_1_17:79479095-79479797:- ENST00000570382.1_1_17:79479095-79479797:- ACTG1 5' Overlap uORF 5' Overlap uORF GN=ACTG1 5' Overlap uORF VH20221207_MKP_FAIMS2022E_HLAc1_4e7_PDAC SCP E_20221201_NO30_400nL_HLAc1_4e7_PDAC_Slot2-6_1_3524.19218.1387.2 2 4 1 11.42 6.81 6.25 4.62 74.2 9 1 1 18000.0 Hydrogen 28 (M) 10 APACAKLVLL (G) 10 APALmITPII A/P/A/C/A\K\L\V\L\L 100.0 10 5 4 0 0 unbiased Carbamidomethylation C:Carbamidomethylation, 66.391502 0.884449 528.3181 1055.628 0.0006 0.5 2.9 8.8 9092.4 nuORFdb_human ENST00000570382.1_1_17:79479095-79479797:- ENST00000570382.1_1_17:79479095-79479797:- ACTG1 5' Overlap uORF 5' Overlap uORF GN=ACTG1 5' Overlap uORF E_20221201_NO30_400nL_HLAc1_4e7_PDAC_Slot2-6_1_3524 Exploris_FAIMS VH20221207_MKP_FAIMS2022E_HLAc1_4e7_PDAC.48751.48751.2 2 1 1 9.74 2.19 9.05 7.56 68.2 8 1 1 12800000.0 Hydrogen 28 (M) 10 APACAKLVLL (G) 10 APGTAKERLL A/P\A-C-A\K\L\V|L/L 100.0 10 5 3 1 2 unbiased Carbamidomethylation C:Carbamidomethylation, 0.998 92.2 86.72 20.86 -70.0 528.3162 1055.628 -0.0031 -2.9 -0.2999999999999998 8.8 9092.4 nuORFdb_human ENST00000570382.1_1_17:79479095-79479797:- ENST00000570382.1_1_17:79479095-79479797:- ACTG1 5' Overlap uORF 5' Overlap uORF GN=ACTG1 5' Overlap uORF VH20221207_MKP_FAIMS2022E_HLAc1_4e7_PDAC SCP E_20221201_NO30_400nL_HLAc1_4e7_PDAC_Slot2-6_1_3524.17865.711.1 1 2 1 10.69 6.09 4.43 4.6 81.0 7 0 0 10400.0 Hydrogen 75 (R) 9 APAGPVGVL (C) 9 APQPGVVL A/P/A/G/P V\G\V\L 77.8 7 3 4 0 0 unbiased 61.718483 1.299817 780.4575 780.461 -0.0044 -5.7 -3.3000000000000003 5.57 11272.8 nuORFdb_human ENST00000343666.5_1_9:140099699-140100026:- ENST00000343666.5_1_9:140099699-140100026:- TMEM203 5' Overlap uORF 5' Overlap uORF GN=TMEM203 5' Overlap uORF E_20221201_NO30_400nL_HLAc1_4e7_PDAC_Slot2-6_1_3524 Exploris_FAIMS VH20221207_MKP_FAIMS2022E_HLAc1_4e7_PDAC.38784.38784.2 2 2 1 12.11 8.88 2.12 3.23 78.5 7 1 1 13400000.0 Hydrogen 129 (A) 9 APAPLRQVL (R) 9 APAGPRLALV A/P|A|P/L/R Q\V/L 77.8 7 3 6 2 0 y-ion bias 0.998 70.2 71.8 120.0 -50.0 482.7987 964.594 -0.0036 -3.8 -1.1999999999999997 9.79 19133.5 nuORFdb_human ENST00000496720.1_1_22:50722364-50724624:- ENST00000496720.1_1_22:50722364-50724624:- PLXNB2 ncRNA Processed Transcript lncRNA GN=PLXNB2 ncRNA Processed Transcript VH20221207_MKP_FAIMS2022E_HLAc1_4e7_PDAC Eva-50mio-3fr AL20210827_EV_HLA_PDAC_PANFRO413_2D_50e6_W632_classI_fxn2.27640.27751.2 2 1 1 14.43 7.13 7.54 7.3 91.1 7 1 1 42700000.0 Hydrogen 8 (R) 10 APFPGRDFQY (L) 10 APFcLQKKSG A/P|F|P/G R D-F\Q|Y 70.0 7 4 5 3 1 unbiased 0.957 95.7 51.17 73.99 0.0 599.288 1197.569 0.0 0.0 -0.3 5.88 2477.9 nuORFdb_human ENST00000561743.5_2_16:58033574-58036432:+ ENST00000561743.5_2_16:58033574-58036432:+|ENST00000219281.7_1_16:58035433-58036432:+|ENST00000561568.5_1_16:58035485-58036432:+ USB1 5' Overlap uORF 5' Overlap uORF GN=USB1 5' Overlap uORF AL20210827_EV_HLA_PDAC_PANFRO413_2D_50e6_W632_classI_fxn2 Exploris AL20221201_MKP_PDAC_01.36279.36415.2 2 1 1 16.98 9.83 7.58 7.15 92.9 6 1 1 370000000.0 Hydrogen 8 (R) 10 APFPGRDFQY (L) 10 APFPTSTTSTST A/P|F|P/G R D F\Q|Y 60.0 6 4 5 3 0 unbiased 0.999 100.0 56.65 73.71 0.0 599.2888 1197.569 0.0017 1.4 0.5999999999999999 5.88 2477.9 nuORFdb_human ENST00000561743.5_2_16:58033574-58036432:+ ENST00000561743.5_2_16:58033574-58036432:+|ENST00000219281.7_1_16:58035433-58036432:+|ENST00000561568.5_1_16:58035485-58036432:+ USB1 5' Overlap uORF 5' Overlap uORF GN=USB1 5' Overlap uORF AL20221201_MKP_PDAC_01 SCP E_20221201_NO30_400nL_HLAc1_4e7_PDAC_Slot2-6_1_3524.17065.1347.2 2 3 1 16.11 6.43 10.71 9.68 91.5 8 1 1 55100.0 Hydrogen 8 (R) 10 APFPGRDFQY (L) 10 APFPTSTTSTST A/P/F|P|G\R D|F\Q\Y 80.0 8 6 5 3 0 unbiased 58.954685 0.909725 599.2864 1197.569 -0.004 -3.3 -0.8999999999999999 5.88 2477.9 nuORFdb_human ENST00000561743.5_2_16:58033574-58036432:+ ENST00000561743.5_2_16:58033574-58036432:+|ENST00000219281.7_1_16:58035433-58036432:+|ENST00000561568.5_1_16:58035485-58036432:+ USB1 5' Overlap uORF 5' Overlap uORF GN=USB1 5' Overlap uORF E_20221201_NO30_400nL_HLAc1_4e7_PDAC_Slot2-6_1_3524 Exploris_FAIMS VH20221207_MKP_FAIMS2022E_HLAc1_4e7_PDAC.42942.43335.2 2 1 1 16.46 8.68 7.16 7.78 93.0 7 1 1 114000000.0 Hydrogen 8 (R) 10 APFPGRDFQY (L) 10 APFPTSTTSTST A/P|F|P/G R D-F\Q|Y 70.0 7 4 5 3 1 unbiased 0.986 95.1 78.37 24.34 -50.0 599.2856 1197.569 -0.0048 -4.0 -1.4 5.88 2477.9 nuORFdb_human ENST00000561743.5_2_16:58033574-58036432:+ ENST00000561743.5_2_16:58033574-58036432:+|ENST00000219281.7_1_16:58035433-58036432:+|ENST00000561568.5_1_16:58035485-58036432:+ USB1 5' Overlap uORF 5' Overlap uORF GN=USB1 5' Overlap uORF VH20221207_MKP_FAIMS2022E_HLAc1_4e7_PDAC Exploris AL20221201_MKP_PDAC_01.17087.17199.3 3 2 1 13.59 9.55 5.27 4.04 86.2 6 2 2 280000000.0 Hydrogen 23 (M) 9 APFPKSRCL (-) 9 APFLRNMIN A/P|F/P/K/S/R C L 66.7 6 1 6 1 0 y-ion bias Carbamidomethylation C:Carbamidomethylation, 0.999 89.4 30.4 22.38 0.0 359.196 1075.572 0.0018 1.7 0.8999999999999999 11.0 3513.2 nuORFdb_human ENST00000489573.5_1_3:160117521-160118495:+ ENST00000489573.5_1_3:160117521-160118495:+ SMC4 5' uORF 5' uORF GN=SMC4 5' uORF AL20221201_MKP_PDAC_01 SCP E_20221201_NO30_400nL_HLAc1_4e7_PDAC_Slot2-6_1_3524.7534.1360.2 2 4 1 13.96 8.17 7.07 5.8 92.6 8 2 2 85200.0 Hydrogen 23 (M) 9 APFPKSRCL (-) 9 APFLRNMIN A/P/F|P|K\S\R-C\L 100.0 9 5 4 2 1 unbiased Carbamidomethylation C:Carbamidomethylation, 26.030725 0.901218 538.2889 1075.572 -0.001 -0.9 1.5 11.0 3513.2 nuORFdb_human ENST00000489573.5_1_3:160117521-160118495:+ ENST00000489573.5_1_3:160117521-160118495:+ SMC4 5' uORF 5' uORF GN=SMC4 5' uORF E_20221201_NO30_400nL_HLAc1_4e7_PDAC_Slot2-6_1_3524 Eva-50mio-3fr AL20210827_EV_HLA_PDAC_PANFRO413_2D_50e6_W632_classI_fxn3.40724.40724.2 2 1 1 10.84 6.09 4.42 4.75 71.4 7 1 1 3660000.0 Hydrogen 92 (G) 9 APGLRLLQL (G) 9 APGAKKPKAI A/P|G/L/R L\L\Q|L 77.8 7 4 5 2 0 unbiased 0.966 94.2 73.87 100.0 0.0 490.8162 980.625 -0.0 -0.0 -0.5 9.79 49147.7 nuORFdb_human ENST00000477647.1_1_3:122514002-122598222:+ ENST00000477647.1_1_3:122514002-122598222:+|ENST00000261038.5_1_3:122514002-122525788:+ DIRC2 3' Overlap dORF 3' Overlap dORF GN=DIRC2 3' Overlap dORF AL20210827_EV_HLA_PDAC_PANFRO413_2D_50e6_W632_classI_fxn3 SCP E_20221201_NO30_400nL_HLAc1_4e7_PDAC_Slot2-6_1_3524.7505.1424.2 2 1 1 13.92 7.67 6.24 6.24 83.3 7 0 0 18800.0 Hydrogen 20 (A) 10 APISSSCHSL (L) 10 APLSnGLQEE A/P/I|S|S|S C H\S\L 70.0 7 5 5 3 0 unbiased Carbamidomethylation C:Carbamidomethylation, 25.930592 0.861218 529.749 1058.494 -0.0028 -2.6 -0.20000000000000018 6.78 3618.1 nuORFdb_human ENST00000304778.11_1_X:118108604-118108709:+ ENST00000304778.11_1_X:118108604-118108709:+ LONRF3 5' uORF 5' uORF GN=LONRF3 5' uORF E_20221201_NO30_400nL_HLAc1_4e7_PDAC_Slot2-6_1_3524 SCP E_20221201_NO30_400nL_HLAc1_4e7_PDAC_Slot2-6_1_3524.10606.1468.2 2 5 1 18.7 14.25 5.39 4.46 93.4 8 1 1 184000.0 Hydrogen 22 (R) 10 APKSSSGFSL (-) 10 APDAAPAPASI A/P/K/S/S S\G|F|S\L 80.0 8 4 6 2 0 unbiased 36.641872 0.833644 490.7544 980.505 -0.004 -4.1 -1.6999999999999997 8.8 3409.0 nuORFdb_human ENST00000565420.5_1_16:18800314-18800410:- ENST00000565420.5_1_16:18800314-18800410:- RPS15A Out-of-Frame Out-of-Frame GN=RPS15A Out-of-Frame E_20221201_NO30_400nL_HLAc1_4e7_PDAC_Slot2-6_1_3524 Exploris_FAIMS VH20221207_MKP_FAIMS2022E_HLAc1_4e7_PDAC.31100.31230.2 2 1 1 15.41 8.55 6.41 6.86 87.2 6 1 1 75000000.0 Hydrogen 22 (R) 10 APKSSSGFSL (-) 10 APDAAPAPASI A P|K\S\S S G\F|S|L 50.0 5 6 3 3 0 b-ion bias 1.0 97.2 60.5 19.24 -50.0 490.7548 980.505 -0.0024 -2.5 0.10000000000000009 8.8 3409.0 nuORFdb_human ENST00000565420.5_1_16:18800314-18800410:- ENST00000565420.5_1_16:18800314-18800410:- RPS15A Out-of-Frame Out-of-Frame GN=RPS15A Out-of-Frame VH20221207_MKP_FAIMS2022E_HLAc1_4e7_PDAC SCP E_20221201_NO30_400nL_HLAc1_4e7_PDAC_Slot2-6_1_3524.8414.1455.2 2 1 1 10.11 7.11 2.27 2.99 78.9 7 1 1 9540.0 Hydrogen 29 (A) 10 APQAPAARAL (P) 10 GAPAAPAALRA A/P/Q|A|P/A/A R\A L 60.0 6 3 6 2 0 y-ion bias 29.068884 0.842137 483.2794 965.553 -0.002 -2.0 0.3999999999999999 9.79 12379.9 nuORFdb_human ENST00000164227.9_1_19:45252055-45254595:+ ENST00000164227.9_1_19:45252055-45254595:+ BCL3 Out-of-Frame Out-of-Frame GN=BCL3 Out-of-Frame E_20221201_NO30_400nL_HLAc1_4e7_PDAC_Slot2-6_1_3524 Exploris_FAIMS VH20221207_MKP_FAIMS2022E_HLAc1_4e7_PDAC.23049.23049.2 2 1 1 12.04 10.04 -0.13 1.99 89.3 6 1 1 5270000.0 Hydrogen 29 (A) 10 APQAPAARAL (P) 10 APAQLRAAPA A/P|Q|A|P/A-A R-A L 60.0 6 3 5 3 2 unbiased 0.999 87.4 47.8 64.91 -70.0 483.2784 965.553 -0.0032 -3.3 -0.6999999999999997 9.79 12379.9 nuORFdb_human ENST00000164227.9_1_19:45252055-45254595:+ ENST00000164227.9_1_19:45252055-45254595:+ BCL3 Out-of-Frame Out-of-Frame GN=BCL3 Out-of-Frame VH20221207_MKP_FAIMS2022E_HLAc1_4e7_PDAC Eva-50mio-3fr AL20210827_EV_HLA_PDAC_PANFRO413_2D_50e6_W632_classI_fxn3.9495.9495.2 2 1 1 12.49 9.44 3.06 3.06 88.8 6 1 2 25100000.0 Hydrogen 17 (A) 9 APRASRPSL (-) 9 APRAAREAL A/P/R|A/S R|P S|L 55.6 5 3 6 3 0 dual b/y bias 0.998 99.4 18.73 26.65 0.0 477.7775 954.548 -0.0002 -0.2 -0.7 12.0 2896.3 nuORFdb_human ENST00000310441.11_1_X:153236587-153236665:- ENST00000310441.11_1_X:153236587-153236665:- HCFC1 5' uORF 5' uORF GN=HCFC1 5' uORF AL20210827_EV_HLA_PDAC_PANFRO413_2D_50e6_W632_classI_fxn3 Exploris AL20221201_MKP_PDAC_01.10645.10758.2 2 1 1 10.66 7.6 3.05 3.05 84.8 6 1 2 670000000.0 Hydrogen 17 (A) 9 APRASRPSL (-) 9 APRAAREAL A/P\R|A/S R|P S\L 55.6 5 4 4 2 0 unbiased 0.999 94.4 20.17 55.8 0.0 477.7778 954.548 0.0003 0.4 -0.4 12.0 2896.3 nuORFdb_human ENST00000310441.11_1_X:153236587-153236665:- ENST00000310441.11_1_X:153236587-153236665:- HCFC1 5' uORF 5' uORF GN=HCFC1 5' uORF AL20221201_MKP_PDAC_01 SCP E_20221201_NO30_400nL_HLAc1_4e7_PDAC_Slot2-6_1_3524.4314.1482.2 2 2 1 10.6 6.55 3.97 4.05 72.3 7 1 2 446000.0 Hydrogen 17 (A) 9 APRASRPSL (-) 9 ARTPGRPSL A/P/R/A/S\R|P S\L 77.8 7 3 5 1 0 unbiased 14.91402 0.825098 477.7765 954.548 -0.0012 -1.3 1.0999999999999999 12.0 2896.3 nuORFdb_human ENST00000310441.11_1_X:153236587-153236665:- ENST00000310441.11_1_X:153236587-153236665:- HCFC1 5' uORF 5' uORF GN=HCFC1 5' uORF E_20221201_NO30_400nL_HLAc1_4e7_PDAC_Slot2-6_1_3524 SCP E_20221201_NO30_400nL_HLAc1_4e7_PDAC_Slot2-6_1_3524.12212.790.1 1 3 1 10.51 6.8 3.23 3.71 80.6 7 0 0 37700.0 Hydrogen 3 (A) 9 APSGVGAAL (G) 9 TAAPAGAAI A/P/S/G V\G\A\A\L 77.8 7 4 3 0 0 unbiased 42.188175 1.251746 742.4067 742.409 -0.0024 -3.2 -0.8000000000000003 5.57 1260.5 nuORFdb_human ENST00000311413.4_1_11:119205243-119205285:+ ENST00000311413.4_1_11:119205243-119205285:+ RNF26 5' uORF 5' uORF GN=RNF26 5' uORF E_20221201_NO30_400nL_HLAc1_4e7_PDAC_Slot2-6_1_3524 SCP E_20221201_NO30_400nL_HLAc1_4e7_PDAC_Slot2-6_1_3524.11676.744.1 1 1 1 11.67 9.49 2.64 2.18 90.1 6 0 0 10200.0 Hydrogen 36 (C) 9 APSSGAVAL (K) 9 AEGAAAVAL A/P/S S G\A\V\A\L 66.7 6 4 2 0 0 b-ion bias 40.337585 1.279531 772.4171 772.42 -0.0029 -3.8 -1.4 5.57 5487.2 nuORFdb_human ENST00000262878.4_1_20:35579850-35580000:- ENST00000262878.4_1_20:35579850-35580000:- SAMHD1 Out-of-Frame Out-of-Frame GN=SAMHD1 Out-of-Frame E_20221201_NO30_400nL_HLAc1_4e7_PDAC_Slot2-6_1_3524 Exploris AL20221201_MKP_PDAC_01.22009.22009.3 3 1 1 9.14 7.35 2.85 1.79 68.1 6 1 1 136000000.0 Hydrogen 3 (A) 10 APVRSHGLVL (-) 10 ARGTYRLVI A/P/V R S H\G\L\V/L 60.0 6 3 3 0 0 unbiased 0.996 62.9 37.6 26.25 0.0 350.214 1048.626 0.0014 1.3 0.5 9.8 1250.5 nuORFdb_human ENST00000236980.10_1_2:207630338-207630374:+ ENST00000236980.10_1_2:207630338-207630374:+ FASTKD2 5' uORF 5' uORF GN=FASTKD2 5' uORF AL20221201_MKP_PDAC_01 SCP E_20221201_NO30_400nL_HLAc1_4e7_PDAC_Slot2-6_1_3524.9035.1381.2 2 1 1 19.94 13.14 5.72 6.8 98.2 8 1 1 17100.0 Hydrogen 3 (A) 10 APVRSHGLVL (-) 10 ARGTYRLVI A/P/V/R/S H|G\L|V\L 80.0 8 4 6 2 0 unbiased 31.213451 0.888478 524.8164 1048.626 -0.0015 -1.4 1.0 9.8 1250.5 nuORFdb_human ENST00000236980.10_1_2:207630338-207630374:+ ENST00000236980.10_1_2:207630338-207630374:+ FASTKD2 5' uORF 5' uORF GN=FASTKD2 5' uORF E_20221201_NO30_400nL_HLAc1_4e7_PDAC_Slot2-6_1_3524 Exploris_FAIMS VH20221207_MKP_FAIMS2022E_HLAc1_4e7_PDAC.25731.25731.2 2 1 1 8.13 1.01 5.49 7.13 65.5 7 1 1 488000.0 Hydrogen 12 (S) 9 APVVQKVQY (I) 9 APVKLFNKD A/P|V|V/Q/K V-Q-Y 77.8 7 2 5 2 2 y-ion bias 0.886 61.5 52.3 120.0 -70.0 516.2966 1031.588 -0.0025 -2.5 0.10000000000000009 8.63 2506.8 nuORFdb_human ENST00000324366.12_2_14:23390027-23390099:- ENST00000324366.12_2_14:23390027-23390099:- PRMT5 3' dORF 3' dORF GN=PRMT5 3' dORF VH20221207_MKP_FAIMS2022E_HLAc1_4e7_PDAC SCP E_20221201_NO30_400nL_HLAc1_4e7_PDAC_Slot2-6_1_3524.7838.1403.2 2 2 1 14.26 7.91 6.92 6.35 91.9 7 1 1 18000.0 Hydrogen 69 (G) 9 APWSCRATC (C) 9 APGTAQSDTTC A/P/W|S|C/R A|T\C 77.8 7 4 6 3 0 dual b/y bias Carbamidomethylation C:Carbamidomethylation, 27.080015 0.874481 554.7364 1108.466 -0.0015 -1.4 1.0 9.79 10718.2 nuORFdb_human ENST00000252485.8_1_19:45349720-45368644:+ ENST00000252485.8_1_19:45349720-45368644:+ NECTIN2 5' Overlap uORF 5' Overlap uORF GN=NECTIN2 5' Overlap uORF E_20221201_NO30_400nL_HLAc1_4e7_PDAC_Slot2-6_1_3524 Eva-50mio-3fr AL20210827_EV_HLA_PDAC_PANFRO413_2D_50e6_W632_classI_fxn1.29850.29850.2 2 1 1 7.92 7.92 7.92 50.0 6 0 0 24800000.0 Hydrogen 26 (C) 9 APYSPPITY (Q) 9 A P\Y S\P\P\I|T/Y 55.6 5 5 2 1 0 b-ion bias 0.988 93.9 53.88 67.72 0.0 504.7576 1008.504 0.0042 4.2 4.1000000000000005 5.57 10393.8 nuORFdb_human TCONS_00020925_12:112279188-112279449:- TCONS_00020925_12:112279188-112279449:- linc-BRAP-2 lincRNA lncRNA GN=linc-BRAP-2 lincRNA AL20210827_EV_HLA_PDAC_PANFRO413_2D_50e6_W632_classI_fxn1 SCP E_20221201_NO30_400nL_HLAc1_4e7_PDAC_Slot2-6_1_3524.18628.398.1 1 7 1 15.7 4.72 8.31 10.98 95.0 8 0 0 57300.0 Hydrogen 26 (C) 9 APYSPPITY (Q) 9 GPYTPDVLF A/P/Y/S|P|P\I\T\Y 100.0 9 5 5 2 0 unbiased 64.353004 1.486257 1008.5014 1008.504 -0.0037 -3.6 -1.2000000000000002 5.57 10393.8 nuORFdb_human TCONS_00020925_12:112279188-112279449:- TCONS_00020925_12:112279188-112279449:- linc-BRAP-2 lincRNA lncRNA GN=linc-BRAP-2 lincRNA E_20221201_NO30_400nL_HLAc1_4e7_PDAC_Slot2-6_1_3524 Exploris_FAIMS VH20221207_MKP_FAIMS2022E_HLAc1_4e7_PDAC.45528.45652.2 2 2 1 12.74 6.68 8.37 6.06 75.9 7 0 0 130000000.0 Hydrogen 26 (C) 9 APYSPPITY (Q) 9 PPSPFSLTY A P\Y-S\P\P\I|T/Y 77.8 7 5 2 1 1 b-ion bias 0.998 81.9 82.33 102.39 -70.0 504.7545 1008.504 -0.002 -2.0 0.6000000000000001 5.57 10393.8 nuORFdb_human TCONS_00020925_12:112279188-112279449:- TCONS_00020925_12:112279188-112279449:- linc-BRAP-2 lincRNA lncRNA GN=linc-BRAP-2 lincRNA VH20221207_MKP_FAIMS2022E_HLAc1_4e7_PDAC 10mio-rep1 E_20221201_NO30_400nL_HLAc1_1e7_directIP_titration_rep1_Slot2-3_1_3512.9095.1557.2 2 2 1 16.89 7.53 6.98 9.36 90.3 8 1 1 10600.0 Hydrogen 21 (S) 9 ASAAVRVAY (R) 9 ASAGGRLIY A/S/A|A|V|R\V\A\Y 100.0 9 6 5 3 0 dual b/y bias 31.423471 0.777814 454.2487 907.5 -0.0089 -9.8 -2.1000000000000005 8.79 3260.6 nuORFdb_human ENST00000427191.6_1_4:87515508-87515601:+ ENST00000427191.6_1_4:87515508-87515601:+ PTPN13 5' uORF 5' uORF GN=PTPN13 5' uORF E_20221201_NO30_400nL_HLAc1_1e7_directIP_titration_rep1_Slot2-3_1_3512 10mio-rep2 E_20221201_NO30_400nL_HLAc1_1e7_directIP_titration_rep2_Slot2-3_1_3513.9043.1558.2 2 3 1 14.42 6.63 7.02 7.78 90.8 8 1 1 10300.0 Hydrogen 21 (S) 9 ASAAVRVAY (R) 9 ASAGGRLIY A/S/A/A|V|R\V\A\Y 100.0 9 5 5 2 0 unbiased 31.243217 0.77677 454.2501 907.5 -0.0069 -7.6 -0.8999999999999995 8.79 3260.6 nuORFdb_human ENST00000427191.6_1_4:87515508-87515601:+ ENST00000427191.6_1_4:87515508-87515601:+ PTPN13 5' uORF 5' uORF GN=PTPN13 5' uORF E_20221201_NO30_400nL_HLAc1_1e7_directIP_titration_rep2_Slot2-3_1_3513 10mio-rep3 E_20221201_NO30_400nL_HLAc1_1e7_directIP_titration_rep3_Slot2-3_1_3514.9053.1589.2 2 3 1 16.66 7.6 7.16 9.07 91.8 8 1 1 14700.0 Hydrogen 21 (S) 9 ASAAVRVAY (R) 9 ASAGGRLIY A/S/A/A|V|R\V\A\Y 100.0 9 5 5 2 0 unbiased 31.280876 0.75757 454.2514 907.5 -0.0049 -5.4 0.8999999999999995 8.79 3260.6 nuORFdb_human ENST00000427191.6_1_4:87515508-87515601:+ ENST00000427191.6_1_4:87515508-87515601:+ PTPN13 5' uORF 5' uORF GN=PTPN13 5' uORF E_20221201_NO30_400nL_HLAc1_1e7_directIP_titration_rep3_Slot2-3_1_3514 20mio-rep2 E_20221201_NO30_400nL_HLAc1_2e7_directIP_titration_rep3_Slot2-4_1_3518.9136.1560.2 2 2 1 18.7 8.52 5.86 10.18 94.7 8 1 1 17500.0 Hydrogen 21 (S) 9 ASAAVRVAY (R) 9 ASAGGRLIY A/S/A/A|V|R|V|A\Y 100.0 9 5 7 4 0 dual b/y bias 31.560482 0.775642 454.2501 907.5 -0.0069 -7.6 -1.2999999999999998 8.79 3260.6 nuORFdb_human ENST00000427191.6_1_4:87515508-87515601:+ ENST00000427191.6_1_4:87515508-87515601:+ PTPN13 5' uORF 5' uORF GN=PTPN13 5' uORF E_20221201_NO30_400nL_HLAc1_2e7_directIP_titration_rep3_Slot2-4_1_3518 20mio-rep3 E_20221201_NO30_400nL_HLAc1_2e7_directIP_titration_rep4_Slot2-4_1_3519.9014.1554.2 2 2 1 14.29 7.3 6.07 6.99 86.9 8 1 1 9680.0 Hydrogen 21 (S) 9 ASAAVRVAY (R) 9 ASAGGRLIY A/S/A/A/V|R\V\A\Y 100.0 9 4 5 1 0 unbiased 31.140568 0.779546 454.2503 907.5 -0.0069 -7.6 -1.7999999999999998 8.79 3260.6 nuORFdb_human ENST00000427191.6_1_4:87515508-87515601:+ ENST00000427191.6_1_4:87515508-87515601:+ PTPN13 5' uORF 5' uORF GN=PTPN13 5' uORF E_20221201_NO30_400nL_HLAc1_2e7_directIP_titration_rep4_Slot2-4_1_3519 40mio-rep1 E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep1_Slot1-04_1_3496.9264.1542.2 2 2 1 21.38 9.5 8.12 11.88 98.1 8 1 1 55700.0 Hydrogen 21 (S) 9 ASAAVRVAY (R) 9 ASAGGRLIY A/S/A|A|V|R|V|A\Y 100.0 9 6 7 5 0 dual b/y bias 32.005291 0.78732 454.2523 907.5 -0.0029 -3.2 0.0 8.79 3260.6 nuORFdb_human ENST00000427191.6_1_4:87515508-87515601:+ ENST00000427191.6_1_4:87515508-87515601:+ PTPN13 5' uORF 5' uORF GN=PTPN13 5' uORF E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep1_Slot1-04_1_3496 40mio-rep2 E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep2_Slot2-5_1_3520.8989.1557.2 2 1 1 18.14 7.91 7.43 10.23 94.0 8 1 1 18600.0 Hydrogen 21 (S) 9 ASAAVRVAY (R) 9 ASAGGRLIY A/S/A/A|V|R|V|A\Y 100.0 9 5 7 4 0 dual b/y bias 31.054499 0.777408 454.2514 907.5 -0.0049 -5.4 -0.5 8.79 3260.6 nuORFdb_human ENST00000427191.6_1_4:87515508-87515601:+ ENST00000427191.6_1_4:87515508-87515601:+ PTPN13 5' uORF 5' uORF GN=PTPN13 5' uORF E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep2_Slot2-5_1_3520 40mio-rep3 E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep3_Slot2-5_1_3521.8989.1557.2 2 1 1 16.87 7.2 6.97 9.67 90.1 8 1 1 17800.0 Hydrogen 21 (S) 9 ASAAVRVAY (R) 9 ASAGGRLIY A/S/A/A|V|R|V|A\Y 100.0 9 5 7 4 0 dual b/y bias 31.056414 0.777738 454.2515 907.5 -0.0049 -5.4 -0.8000000000000007 8.79 3260.6 nuORFdb_human ENST00000427191.6_1_4:87515508-87515601:+ ENST00000427191.6_1_4:87515508-87515601:+ PTPN13 5' uORF 5' uORF GN=PTPN13 5' uORF E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep3_Slot2-5_1_3521 40mio-rep4 E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep4_Slot2-5_1_3522.9002.1556.2 2 3 1 19.81 8.64 6.49 11.17 89.6 8 1 1 55700.0 Hydrogen 21 (S) 9 ASAAVRVAY (R) 9 ASAGGRLIY A/S/A|A|V|R|V|A\Y 100.0 9 6 7 5 0 dual b/y bias 31.091602 0.777933 454.2508 907.5 -0.0049 -5.4 -0.7000000000000002 8.79 3260.6 nuORFdb_human ENST00000427191.6_1_4:87515508-87515601:+ ENST00000427191.6_1_4:87515508-87515601:+ PTPN13 5' uORF 5' uORF GN=PTPN13 5' uORF E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep4_Slot2-5_1_3522 5mio-rep1 E_20221201_NO30_400nL_HLAc1_5e6_directIP_titration_rep1_Slot1-37_1_3502.9301.1578.2 2 3 1 11.96 3.78 11.96 73.9 7 1 1 11200.0 Hydrogen 21 (S) 9 ASAAVRVAY (R) 9 A S/A/A|V|R\V|A\Y 77.8 7 5 5 3 0 dual b/y bias 32.132042 0.764306 454.25 907.5 -0.0069 -7.6 -0.5999999999999996 8.79 3260.6 nuORFdb_human ENST00000427191.6_1_4:87515508-87515601:+ ENST00000427191.6_1_4:87515508-87515601:+ PTPN13 5' uORF 5' uORF GN=PTPN13 5' uORF E_20221201_NO30_400nL_HLAc1_5e6_directIP_titration_rep1_Slot1-37_1_3502 5mio-rep2 E_20221201_NO30_400nL_HLAc1_5e6_directIP_titration_rep2_Slot2-2_1_3510.9271.1570.2 2 1 1 12.15 4.87 4.74 7.28 72.1 8 1 1 3480.0 Hydrogen 21 (S) 9 ASAAVRVAY (R) 9 ASAGGRLIY A/S/A/A/V/R\V|A\Y 100.0 9 3 6 1 0 y-ion bias 32.020645 0.769034 454.2483 907.5 -0.0109 -12.0 -3.3000000000000007 8.79 3260.6 nuORFdb_human ENST00000427191.6_1_4:87515508-87515601:+ ENST00000427191.6_1_4:87515508-87515601:+ PTPN13 5' uORF 5' uORF GN=PTPN13 5' uORF E_20221201_NO30_400nL_HLAc1_5e6_directIP_titration_rep2_Slot2-2_1_3510 10mio-rep1 E_20221201_NO30_400nL_HLAc1_1e7_directIP_titration_rep1_Slot2-3_1_3512.25488.542.1 1 1 1 10.64 4.86 4.5 5.78 86.4 5 0 0 1830.0 Hydrogen 33 (Q) 9 ASAPLVAVW (-) 9 AVPALSGLAD A S A/P L\V\A\V\W 44.4 4 4 1 0 0 b-ion bias 88.038727 1.401035 913.5056 913.514 -0.0082 -9.0 -1.2999999999999998 5.57 4530.1 nuORFdb_human ENST00000367137.3_1_1:205779798-205779921:- ENST00000367137.3_1_1:205779798-205779921:- SLC41A1 5' uORF 5' uORF GN=SLC41A1 5' uORF E_20221201_NO30_400nL_HLAc1_1e7_directIP_titration_rep1_Slot2-3_1_3512 10mio-rep2 E_20221201_NO30_400nL_HLAc1_1e7_directIP_titration_rep2_Slot2-3_1_3513.25411.543.1 1 2 1 11.78 4.68 6.29 7.1 86.2 6 0 0 3500.0 Hydrogen 33 (Q) 9 ASAPLVAVW (-) 9 AIPASIGVW A S/A/P L|V\A\V\W 55.6 5 4 3 1 0 unbiased 87.765305 1.400531 913.5068 913.514 -0.0072 -7.9 -1.2000000000000002 5.57 4530.1 nuORFdb_human ENST00000367137.3_1_1:205779798-205779921:- ENST00000367137.3_1_1:205779798-205779921:- SLC41A1 5' uORF 5' uORF GN=SLC41A1 5' uORF E_20221201_NO30_400nL_HLAc1_1e7_directIP_titration_rep2_Slot2-3_1_3513 20mio-rep1 E_20221201_NO30_400nL_HLAc1_2e7_directIP_titration_rep1_Slot2-4_1_3515.25372.544.1 1 1 1 10.38 4.01 4.35 6.38 86.2 5 0 0 3820.0 Hydrogen 33 (Q) 9 ASAPLVAVW (-) 9 AVPALSGLAD A S A/P L\V\A\V\W 44.4 4 4 1 0 0 b-ion bias 87.614601 1.399757 913.5071 913.514 -0.0072 -7.9 -1.7000000000000002 5.57 4530.1 nuORFdb_human ENST00000367137.3_1_1:205779798-205779921:- ENST00000367137.3_1_1:205779798-205779921:- SLC41A1 5' uORF 5' uORF GN=SLC41A1 5' uORF E_20221201_NO30_400nL_HLAc1_2e7_directIP_titration_rep1_Slot2-4_1_3515 20mio-rep2 E_20221201_NO30_400nL_HLAc1_2e7_directIP_titration_rep3_Slot2-4_1_3518.25406.533.1 1 1 1 10.47 4.7 5.53 5.76 88.6 5 0 0 3180.0 Hydrogen 33 (Q) 9 ASAPLVAVW (-) 9 AAVTPAVGLD A S A/P L\V\A\V\W 44.4 4 4 1 0 0 b-ion bias 87.751495 1.406085 913.5092 913.514 -0.0052 -5.7 0.5999999999999996 5.57 4530.1 nuORFdb_human ENST00000367137.3_1_1:205779798-205779921:- ENST00000367137.3_1_1:205779798-205779921:- SLC41A1 5' uORF 5' uORF GN=SLC41A1 5' uORF E_20221201_NO30_400nL_HLAc1_2e7_directIP_titration_rep3_Slot2-4_1_3518 20mio-rep3 E_20221201_NO30_400nL_HLAc1_2e7_directIP_titration_rep4_Slot2-4_1_3519.25433.540.1 1 3 1 13.24 4.63 6.01 8.61 92.7 6 0 0 7030.0 Hydrogen 33 (Q) 9 ASAPLVAVW (-) 9 GLVPATAVEG A S/A/P L|V\A\V\W 55.6 5 4 3 1 0 unbiased 87.838387 1.402123 913.507 913.514 -0.0072 -7.9 -2.1000000000000005 5.57 4530.1 nuORFdb_human ENST00000367137.3_1_1:205779798-205779921:- ENST00000367137.3_1_1:205779798-205779921:- SLC41A1 5' uORF 5' uORF GN=SLC41A1 5' uORF E_20221201_NO30_400nL_HLAc1_2e7_directIP_titration_rep4_Slot2-4_1_3519 40mio-rep1 E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep1_Slot1-04_1_3496.25607.513.1 1 2 1 12.72 5.75 6.0 6.97 94.1 5 0 0 22500.0 Hydrogen 33 (Q) 9 ASAPLVAVW (-) 9 AVPALSGLAD A S A/P L\V\A\V\W 44.4 4 4 1 0 0 b-ion bias 88.461739 1.418278 913.5088 913.514 -0.0052 -5.7 -2.5 5.57 4530.1 nuORFdb_human ENST00000367137.3_1_1:205779798-205779921:- ENST00000367137.3_1_1:205779798-205779921:- SLC41A1 5' uORF 5' uORF GN=SLC41A1 5' uORF E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep1_Slot1-04_1_3496 40mio-rep3 E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep3_Slot2-5_1_3521.25393.530.1 1 1 1 11.71 3.83 5.89 7.88 88.2 7 0 0 5160.0 Hydrogen 33 (Q) 9 ASAPLVAVW (-) 9 GLVPATAVEG A S/A/P\L\V\A\V\W 77.8 7 5 2 0 0 b-ion bias 87.717064 1.407902 913.5077 913.514 -0.0062 -6.8 -2.2 5.57 4530.1 nuORFdb_human ENST00000367137.3_1_1:205779798-205779921:- ENST00000367137.3_1_1:205779798-205779921:- SLC41A1 5' uORF 5' uORF GN=SLC41A1 5' uORF E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep3_Slot2-5_1_3521 40mio-rep4 E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep4_Slot2-5_1_3522.25403.527.1 1 2 1 14.98 5.86 7.61 9.12 93.0 6 0 0 9160.0 Hydrogen 33 (Q) 9 ASAPLVAVW (-) 9 AIPASIGVW A S/A/P L|V\A|V\W 55.6 5 4 4 2 0 unbiased 87.741951 1.409909 913.5105 913.514 -0.0042 -4.6 0.10000000000000053 5.57 4530.1 nuORFdb_human ENST00000367137.3_1_1:205779798-205779921:- ENST00000367137.3_1_1:205779798-205779921:- SLC41A1 5' uORF 5' uORF GN=SLC41A1 5' uORF E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep4_Slot2-5_1_3522 40mio-rep1 E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep1_Slot1-04_1_3496.20186.469.1 1 3 1 13.47 4.4 5.72 9.06 85.6 7 0 0 11100.0 M15m Hydrogen 13 (S) 9 ASmEPAGFW (C) 9 DMTSERSW A S/m/E|P\A\G\F\W 77.8 7 5 3 1 0 unbiased m:Oxidized methionine 69.732826 1.444614 1011.4231 995.429 15.9909 -4.0 -0.7999999999999998 4.0 8956.2 nuORFdb_human ENST00000445399.5_1_2:70683474-70692835:- ENST00000445399.5_1_2:70683474-70692835:- TGFA Out-of-Frame Out-of-Frame GN=TGFA Out-of-Frame E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep1_Slot1-04_1_3496 40mio-rep2 E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep2_Slot2-5_1_3520.19883.482.1 1 2 1 12.26 4.78 5.16 7.48 84.4 6 0 0 7580.0 M15m Hydrogen 13 (S) 9 ASmEPAGFW (C) 9 mATRSSDW A S m/E|P\A\G\F\W 66.7 6 5 2 1 0 b-ion bias m:Oxidized methionine 68.686203 1.436427 1011.4191 995.429 15.9909 -4.0 0.9000000000000004 4.0 8956.2 nuORFdb_human ENST00000445399.5_1_2:70683474-70692835:- ENST00000445399.5_1_2:70683474-70692835:- TGFA Out-of-Frame Out-of-Frame GN=TGFA Out-of-Frame E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep2_Slot2-5_1_3520 40mio-rep3 E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep3_Slot2-5_1_3521.19933.484.1 1 4 1 12.11 3.78 5.25 8.33 82.6 7 0 0 11600.0 M15m Hydrogen 13 (S) 9 ASmEPAGFW (C) 9 MSSEEDKSV A S/m/E/P\A\G\F\W 77.8 7 4 3 0 0 unbiased m:Oxidized methionine 68.858795 1.435595 1011.419 995.429 15.9909 -4.0 0.5999999999999996 4.0 8956.2 nuORFdb_human ENST00000445399.5_1_2:70683474-70692835:- ENST00000445399.5_1_2:70683474-70692835:- TGFA Out-of-Frame Out-of-Frame GN=TGFA Out-of-Frame E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep3_Slot2-5_1_3521 40mio-rep4 E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep4_Slot2-5_1_3522.19910.486.1 1 2 1 11.97 3.66 5.22 8.3 80.3 7 0 0 6330.0 M15m Hydrogen 13 (S) 9 ASmEPAGFW (C) 9 mPFDGnKW A S/m/E|P\A\G\F\W 77.8 7 5 3 1 0 unbiased m:Oxidized methionine 68.762306 1.434505 1011.4211 995.429 15.9909 -4.0 0.7000000000000002 4.0 8956.2 nuORFdb_human ENST00000445399.5_1_2:70683474-70692835:- ENST00000445399.5_1_2:70683474-70692835:- TGFA Out-of-Frame Out-of-Frame GN=TGFA Out-of-Frame E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep4_Slot2-5_1_3522 10mio-rep1 E_20221201_NO30_400nL_HLAc1_1e7_directIP_titration_rep1_Slot2-3_1_3512.14348.1486.2 2 3 1 12.14 5.41 6.33 6.73 78.8 8 1 1 18900.0 Hydrogen 65 (T) 9 ASSTAFRIY (-) 9 EAGLRAGSPSA A/S/S/T/A|F/R|I\Y 100.0 9 3 7 2 0 y-ion bias 49.566349 0.822078 508.2599 1015.521 -0.008 -7.9 -0.20000000000000018 8.79 8754.0 nuORFdb_human ENST00000507606.2_1_7:135347283-135358947:+ ENST00000507606.2_1_7:135347283-135358947:+ C7orf73 5' Overlap uORF 5' Overlap uORF GN=C7orf73 5' Overlap uORF E_20221201_NO30_400nL_HLAc1_1e7_directIP_titration_rep1_Slot2-3_1_3512 10mio-rep2 E_20221201_NO30_400nL_HLAc1_1e7_directIP_titration_rep2_Slot2-3_1_3513.14305.1486.2 2 4 1 13.63 8.6 5.45 5.03 85.4 8 1 1 23300.0 Hydrogen 65 (T) 9 ASSTAFRIY (-) 9 EAGLRAGSPSA A/S/S/T/A|F/R|I\Y 100.0 9 3 7 2 0 y-ion bias 49.417667 0.82253 508.261 1015.521 -0.006 -5.9 0.7999999999999998 8.79 8754.0 nuORFdb_human ENST00000507606.2_1_7:135347283-135358947:+ ENST00000507606.2_1_7:135347283-135358947:+ C7orf73 5' Overlap uORF 5' Overlap uORF GN=C7orf73 5' Overlap uORF E_20221201_NO30_400nL_HLAc1_1e7_directIP_titration_rep2_Slot2-3_1_3513 10mio-rep3 E_20221201_NO30_400nL_HLAc1_1e7_directIP_titration_rep3_Slot2-3_1_3514.14260.1515.2 2 2 1 14.54 7.29 7.55 7.25 84.5 8 1 1 14400.0 Hydrogen 65 (T) 9 ASSTAFRIY (-) 9 EAGLRAGSPSA A/S/S/T|A|F/R|I\Y 100.0 9 4 7 3 0 dual b/y bias 49.264378 0.80428 508.2601 1015.521 -0.008 -7.9 -1.6000000000000005 8.79 8754.0 nuORFdb_human ENST00000507606.2_1_7:135347283-135358947:+ ENST00000507606.2_1_7:135347283-135358947:+ C7orf73 5' Overlap uORF 5' Overlap uORF GN=C7orf73 5' Overlap uORF E_20221201_NO30_400nL_HLAc1_1e7_directIP_titration_rep3_Slot2-3_1_3514 20mio-rep1 E_20221201_NO30_400nL_HLAc1_2e7_directIP_titration_rep1_Slot2-4_1_3515.14249.1482.2 2 4 1 17.83 10.31 8.85 7.51 88.0 8 1 1 25800.0 Hydrogen 65 (T) 9 ASSTAFRIY (-) 9 EAGLRAGSPSA A/S/S/T|A|F/R|I\Y 100.0 9 4 7 3 0 dual b/y bias 49.213829 0.824827 508.2616 1015.521 -0.004 -3.9 2.3000000000000003 8.79 8754.0 nuORFdb_human ENST00000507606.2_1_7:135347283-135358947:+ ENST00000507606.2_1_7:135347283-135358947:+ C7orf73 5' Overlap uORF 5' Overlap uORF GN=C7orf73 5' Overlap uORF E_20221201_NO30_400nL_HLAc1_2e7_directIP_titration_rep1_Slot2-4_1_3515 20mio-rep2 E_20221201_NO30_400nL_HLAc1_2e7_directIP_titration_rep3_Slot2-4_1_3518.14369.1480.2 2 4 1 16.06 6.84 10.72 9.22 85.6 7 1 1 43900.0 Hydrogen 65 (T) 9 ASSTAFRIY (-) 9 EAGLRAGSPSA A S/S/T/A|F|R|I\Y 77.8 7 4 6 3 0 dual b/y bias 49.632694 0.826067 508.261 1015.521 -0.006 -5.9 0.39999999999999947 8.79 8754.0 nuORFdb_human ENST00000507606.2_1_7:135347283-135358947:+ ENST00000507606.2_1_7:135347283-135358947:+ C7orf73 5' Overlap uORF 5' Overlap uORF GN=C7orf73 5' Overlap uORF E_20221201_NO30_400nL_HLAc1_2e7_directIP_titration_rep3_Slot2-4_1_3518 20mio-rep3 E_20221201_NO30_400nL_HLAc1_2e7_directIP_titration_rep4_Slot2-4_1_3519.14281.1480.2 2 3 1 14.26 7.58 8.38 6.68 80.7 8 1 1 38300.0 Hydrogen 65 (T) 9 ASSTAFRIY (-) 9 EAGLRAGSPSA A/S/S/T|A|F/R\I\Y 100.0 9 4 6 2 0 unbiased 49.327827 0.826169 508.2612 1015.521 -0.006 -5.9 -0.10000000000000053 8.79 8754.0 nuORFdb_human ENST00000507606.2_1_7:135347283-135358947:+ ENST00000507606.2_1_7:135347283-135358947:+ C7orf73 5' Overlap uORF 5' Overlap uORF GN=C7orf73 5' Overlap uORF E_20221201_NO30_400nL_HLAc1_2e7_directIP_titration_rep4_Slot2-4_1_3519 40mio-rep1 E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep1_Slot1-04_1_3496.14541.1459.2 2 2 1 16.56 6.99 11.26 9.57 85.8 8 1 1 58100.0 Hydrogen 65 (T) 9 ASSTAFRIY (-) 9 EAGLRAGSPSA A/S/S/T|A|F/R|I\Y 100.0 9 4 7 3 0 dual b/y bias 50.229099 0.839606 508.2617 1015.521 -0.004 -3.9 -0.6999999999999997 8.79 8754.0 nuORFdb_human ENST00000507606.2_1_7:135347283-135358947:+ ENST00000507606.2_1_7:135347283-135358947:+ C7orf73 5' Overlap uORF 5' Overlap uORF GN=C7orf73 5' Overlap uORF E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep1_Slot1-04_1_3496 40mio-rep2 E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep2_Slot2-5_1_3520.14225.1470.2 2 1 1 15.48 8.35 6.79 7.13 85.1 8 1 1 36400.0 Hydrogen 65 (T) 9 ASSTAFRIY (-) 9 EAGLRAGSPSA A/S/S/T|A|F/R|I\Y 100.0 9 4 7 3 0 dual b/y bias 49.139145 0.832494 508.2612 1015.521 -0.006 -5.9 -1.0 8.79 8754.0 nuORFdb_human ENST00000507606.2_1_7:135347283-135358947:+ ENST00000507606.2_1_7:135347283-135358947:+ C7orf73 5' Overlap uORF 5' Overlap uORF GN=C7orf73 5' Overlap uORF E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep2_Slot2-5_1_3520 40mio-rep3 E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep3_Slot2-5_1_3521.14246.1506.2 2 3 1 14.47 8.43 7.78 6.04 85.0 8 1 1 39400.0 Hydrogen 65 (T) 9 ASSTAFRIY (-) 9 EAGLRAGSPSA A/S/S/T/A|F|R|I\Y 100.0 9 4 7 3 0 dual b/y bias 49.214916 0.809639 508.2618 1015.521 -0.004 -3.9 0.6999999999999997 8.79 8754.0 nuORFdb_human ENST00000507606.2_1_7:135347283-135358947:+ ENST00000507606.2_1_7:135347283-135358947:+ C7orf73 5' Overlap uORF 5' Overlap uORF GN=C7orf73 5' Overlap uORF E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep3_Slot2-5_1_3521 40mio-rep4 E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep4_Slot2-5_1_3522.14214.1472.2 2 1 1 13.8 7.25 6.98 6.55 79.9 8 1 1 17400.0 Hydrogen 65 (T) 9 ASSTAFRIY (-) 9 EAGLRAGSPSA A/S/S/T|A|F/R\I\Y 100.0 9 4 6 2 0 unbiased 49.090393 0.831239 508.2615 1015.521 -0.004 -3.9 0.8000000000000003 8.79 8754.0 nuORFdb_human ENST00000507606.2_1_7:135347283-135358947:+ ENST00000507606.2_1_7:135347283-135358947:+ C7orf73 5' Overlap uORF 5' Overlap uORF GN=C7orf73 5' Overlap uORF E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep4_Slot2-5_1_3522 5mio-rep1 E_20221201_NO30_400nL_HLAc1_5e6_directIP_titration_rep1_Slot1-37_1_3502.14612.1477.2 2 3 1 14.92 7.66 8.3 7.26 79.1 8 1 1 12900.0 Hydrogen 65 (T) 9 ASSTAFRIY (-) 9 SnAVPKAEAGA A/S/S/T|A|F/R|I\Y 100.0 9 4 7 3 0 dual b/y bias 50.473713 0.828307 508.2609 1015.521 -0.006 -5.9 1.0999999999999996 8.79 8754.0 nuORFdb_human ENST00000507606.2_1_7:135347283-135358947:+ ENST00000507606.2_1_7:135347283-135358947:+ C7orf73 5' Overlap uORF 5' Overlap uORF GN=C7orf73 5' Overlap uORF E_20221201_NO30_400nL_HLAc1_5e6_directIP_titration_rep1_Slot1-37_1_3502 5mio-rep2 E_20221201_NO30_400nL_HLAc1_5e6_directIP_titration_rep2_Slot2-2_1_3510.14496.1486.2 2 3 1 13.97 7.35 7.06 6.63 81.8 8 1 1 9410.0 Hydrogen 65 (T) 9 ASSTAFRIY (-) 9 EAGLRAGSPSA A/S/S/T/A|F/R|I\Y 100.0 9 3 7 2 0 y-ion bias 50.068787 0.82248 508.2594 1015.521 -0.01 -9.8 -1.1000000000000014 8.79 8754.0 nuORFdb_human ENST00000507606.2_1_7:135347283-135358947:+ ENST00000507606.2_1_7:135347283-135358947:+ C7orf73 5' Overlap uORF 5' Overlap uORF GN=C7orf73 5' Overlap uORF E_20221201_NO30_400nL_HLAc1_5e6_directIP_titration_rep2_Slot2-2_1_3510 5mio-rep3 E_20221201_NO30_400nL_HLAc1_5e6_directIP_titration_rep3_Slot2-2_1_3511.14526.1499.2 2 3 1 13.35 6.72 8.15 6.63 81.5 8 1 1 11100.0 Hydrogen 65 (T) 9 ASSTAFRIY (-) 9 EAGLRAGSPSA A/S/S/T|A/F/R|I\Y 100.0 9 3 7 2 0 y-ion bias 50.177784 0.814136 508.2593 1015.521 -0.01 -9.8 -1.6000000000000014 8.79 8754.0 nuORFdb_human ENST00000507606.2_1_7:135347283-135358947:+ ENST00000507606.2_1_7:135347283-135358947:+ C7orf73 5' Overlap uORF 5' Overlap uORF GN=C7orf73 5' Overlap uORF E_20221201_NO30_400nL_HLAc1_5e6_directIP_titration_rep3_Slot2-2_1_3511 40mio-rep2 E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep2_Slot2-5_1_3520.20873.587.1 1 1 1 11.18 9.13 1.02 2.05 83.1 6 0 0 5580.0 Hydrogen 8 (V) 8 ASVIPQSW (A) 8 SVALPSQW A S/V/I|P|Q\S\W 75.0 6 4 4 2 0 unbiased 72.108154 1.374193 887.4563 887.462 -0.0061 -6.9 -2.0 5.57 8543.6 smORF_v4|nuORFdb_human ZNF790-AS1_19:37288446-37288670:+ ZNF790-AS1_19:37288446-37288670:+|ENST00000587413.5_1_19:37288433-37288667:+|ENST00000589218.5_1_19:37808810-37809047:+ ZNF790-AS1 lncRNA 19:37288446-37288670:+ 19 ZNF790-AS1 Antisense lncRNA GN=ZNF790-AS1 E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep2_Slot2-5_1_3520 40mio-rep4 E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep4_Slot2-5_1_3522.20894.584.1 1 1 1 11.53 7.89 1.01 3.64 86.9 6 0 0 5060.0 Hydrogen 8 (V) 8 ASVIPQSW (A) 8 VSLAPQSW A S/V|I/P/Q|S\W 75.0 6 3 5 2 0 unbiased 72.160652 1.3758 887.4592 887.462 -0.0031 -3.5 1.2000000000000002 5.57 8543.6 smORF_v4|nuORFdb_human ZNF790-AS1_19:37288446-37288670:+ ZNF790-AS1_19:37288446-37288670:+|ENST00000587413.5_1_19:37288433-37288667:+|ENST00000589218.5_1_19:37808810-37809047:+ ZNF790-AS1 lncRNA 19:37288446-37288670:+ 19 ZNF790-AS1 Antisense lncRNA GN=ZNF790-AS1 E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep4_Slot2-5_1_3522 10mio-rep1 E_20221201_NO30_400nL_HLAc1_1e7_directIP_titration_rep1_Slot2-3_1_3512.18976.1376.2 2 1 1 15.37 5.97 9.4 9.4 84.1 9 1 1 3660.0 Hydrogen 26 (A) 11 ASVLLAGARNW (W) 11 ISVAIAHGVFSG A S/V/L|L/A|G/A|R|N-W 81.8 9 4 8 4 1 dual b/y bias 65.552422 0.891094 579.3195 1157.643 -0.0118 -10.2 -2.499999999999999 9.79 13779.6 smORF_v4|nuORFdb_human LINC00888_3:183165411-183170082:+ LINC00888_3:183165411-183170082:+|ENST00000471496.5_1_3:183165407-183170079:+|ENST00000468380.1_1_3:183165467-183165554:+ LINC00888 lncRNA 3:183165411-183170082:+ 3 LINC00888 Pseudogene Pseudogene GN=LINC00888 E_20221201_NO30_400nL_HLAc1_1e7_directIP_titration_rep1_Slot2-3_1_3512 10mio-rep3 E_20221201_NO30_400nL_HLAc1_1e7_directIP_titration_rep3_Slot2-3_1_3514.18866.1376.2 2 1 1 13.11 4.96 7.98 8.14 79.4 9 1 1 2870.0 Hydrogen 26 (A) 11 ASVLLAGARNW (W) 11 mAKQAQIPLAA A S/V/L|L|A/G/A\R/N-W 81.8 9 3 7 2 1 y-ion bias 65.169121 0.891206 579.323 1157.643 -0.0038 -3.3 3.0 9.79 13779.6 smORF_v4|nuORFdb_human LINC00888_3:183165411-183170082:+ LINC00888_3:183165411-183170082:+|ENST00000471496.5_1_3:183165407-183170079:+|ENST00000468380.1_1_3:183165467-183165554:+ LINC00888 lncRNA 3:183165411-183170082:+ 3 LINC00888 Pseudogene Pseudogene GN=LINC00888 E_20221201_NO30_400nL_HLAc1_1e7_directIP_titration_rep3_Slot2-3_1_3514 20mio-rep1 E_20221201_NO30_400nL_HLAc1_2e7_directIP_titration_rep1_Slot2-4_1_3515.18907.1370.2 2 1 1 17.97 7.95 8.8 10.02 92.9 8 1 1 5180.0 Hydrogen 26 (A) 11 ASVLLAGARNW (W) 11 GLSLILQnGLE A S V|L|L|A|G|A/R|N\W 72.7 8 7 7 6 0 dual b/y bias 65.295738 0.89505 579.3208 1157.643 -0.0078 -6.8 -0.5999999999999996 9.79 13779.6 smORF_v4|nuORFdb_human LINC00888_3:183165411-183170082:+ LINC00888_3:183165411-183170082:+|ENST00000471496.5_1_3:183165407-183170079:+|ENST00000468380.1_1_3:183165467-183165554:+ LINC00888 lncRNA 3:183165411-183170082:+ 3 LINC00888 Pseudogene Pseudogene GN=LINC00888 E_20221201_NO30_400nL_HLAc1_2e7_directIP_titration_rep1_Slot2-4_1_3515 20mio-rep2 E_20221201_NO30_400nL_HLAc1_2e7_directIP_titration_rep3_Slot2-4_1_3518.18969.1374.2 2 1 1 17.01 7.93 8.1 9.08 89.8 8 1 1 5570.0 Hydrogen 26 (A) 11 ASVLLAGARNW (W) 11 ISVAIAHGVFSG A S/V|L|L|A|G/A R|N\W 63.6 7 6 7 5 0 dual b/y bias 65.519608 0.892356 579.3211 1157.643 -0.0078 -6.8 -0.5 9.79 13779.6 smORF_v4|nuORFdb_human LINC00888_3:183165411-183170082:+ LINC00888_3:183165411-183170082:+|ENST00000471496.5_1_3:183165407-183170079:+|ENST00000468380.1_1_3:183165467-183165554:+ LINC00888 lncRNA 3:183165411-183170082:+ 3 LINC00888 Pseudogene Pseudogene GN=LINC00888 E_20221201_NO30_400nL_HLAc1_2e7_directIP_titration_rep3_Slot2-4_1_3518 20mio-rep3 E_20221201_NO30_400nL_HLAc1_2e7_directIP_titration_rep4_Slot2-4_1_3519.18975.1374.2 2 2 1 14.07 6.32 7.75 7.75 83.4 7 1 1 9170.0 Hydrogen 26 (A) 11 ASVLLAGARNW (W) 11 ISVAIAHGVFSG A S V/L|L|A|G/A|R\N W 54.5 6 5 6 4 0 dual b/y bias 65.536415 0.892468 579.3215 1157.643 -0.0078 -6.8 -1.0 9.79 13779.6 smORF_v4|nuORFdb_human LINC00888_3:183165411-183170082:+ LINC00888_3:183165411-183170082:+|ENST00000471496.5_1_3:183165407-183170079:+|ENST00000468380.1_1_3:183165467-183165554:+ LINC00888 lncRNA 3:183165411-183170082:+ 3 LINC00888 Pseudogene Pseudogene GN=LINC00888 E_20221201_NO30_400nL_HLAc1_2e7_directIP_titration_rep4_Slot2-4_1_3519 40mio-rep1 E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep1_Slot1-04_1_3496.19058.1345.2 2 1 1 7.05 2.26 4.79 4.79 55.9 7 1 1 9560.0 Hydrogen 26 (A) 11 ASVLLAGARNW (W) 11 ISVAIAHGVFSG A S V/L/L|A|G/A R\N\W 54.5 6 4 5 2 0 unbiased 65.836533 0.910785 579.3254 1157.643 0.0002 0.1 3.3000000000000003 9.79 13779.6 smORF_v4|nuORFdb_human LINC00888_3:183165411-183170082:+ LINC00888_3:183165411-183170082:+|ENST00000471496.5_1_3:183165407-183170079:+|ENST00000468380.1_1_3:183165467-183165554:+ LINC00888 lncRNA 3:183165411-183170082:+ 3 LINC00888 Pseudogene Pseudogene GN=LINC00888 E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep1_Slot1-04_1_3496 40mio-rep4 E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep4_Slot2-5_1_3522.6472.1668.3 3 1 1 10.11 8.58 1.53 1.52 92.9 5 2 2 91500.0 Hydrogen 23 (K) 9 ASWKVFRFL (Y) 9 VSWAKRFFI A S W K\V\F\R|F\L 55.6 5 5 1 1 0 b-ion bias 22.356596 0.70736 385.219 1153.652 -0.0092 -8.0 -3.3 11.0 13145.7 nuORFdb_human ENST00000429289.5_1_7:143216320-143216668:+ ENST00000429289.5_1_7:143216320-143216668:+ EPHA1-AS1 Antisense lncRNA GN=EPHA1-AS1 Antisense E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep4_Slot2-5_1_3522 20mio-rep3 E_20221201_NO30_400nL_HLAc1_2e7_directIP_titration_rep4_Slot2-4_1_3519.14773.1503.2 2 1 1 7.94 3.85 2.42 4.09 59.2 6 1 1 3840.0 Hydrogen 15 (A) 9 ATAFNKAVW (F) 9 KnELFAVW A/T/A/F/N K A|V\W 66.7 6 2 5 1 0 y-ion bias 51.026474 0.811597 504.264 1007.531 -0.0102 -10.1 -4.3 8.8 4387.9 smORF_v4|nuORFdb_human IKZF5_10:124768210-124766692:+ IKZF5_10:124768210-124766692:+|ENST00000469821.1_1_10:124758154-124768281:-|ENST00000368886.9_1_10:124766662-124768305:- IKZF5 uORF 10:124768210-124766692:+ 10 IKZF5 5' uORF 5' uORF GN=IKZF5 E_20221201_NO30_400nL_HLAc1_2e7_directIP_titration_rep4_Slot2-4_1_3519 40mio-rep1 E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep1_Slot1-04_1_3496.14997.1465.2 2 1 1 7.39 2.73 2.83 4.66 57.0 5 1 1 16200.0 Hydrogen 15 (A) 9 ATAFNKAVW (F) 9 GFALSTAMLP A T/A/F/N K A\V\W 44.4 4 2 3 0 0 unbiased 51.804066 0.835691 504.2674 1007.531 -0.0042 -4.1 -0.8999999999999995 8.8 4387.9 smORF_v4|nuORFdb_human IKZF5_10:124768210-124766692:+ IKZF5_10:124768210-124766692:+|ENST00000469821.1_1_10:124758154-124768281:-|ENST00000368886.9_1_10:124766662-124768305:- IKZF5 uORF 10:124768210-124766692:+ 10 IKZF5 5' uORF 5' uORF GN=IKZF5 E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep1_Slot1-04_1_3496 20mio-rep2 E_20221201_NO30_400nL_HLAc1_2e7_directIP_titration_rep3_Slot2-4_1_3518.6115.1525.2 2 1 1 7.62 5.42 2.05 2.2 69.1 6 1 1 2620.0 Hydrogen 27 (R) 9 ATARGSPSW (A) 9 nSHGILSGF A/T/A/R G/S|P S\W 55.6 5 2 5 1 0 y-ion bias 21.130253 0.79779 466.7292 932.458 -0.0077 -8.3 -2.000000000000001 9.79 9562.7 nuORFdb_human ENST00000393655.3_1_6:50786629-50791321:+ ENST00000393655.3_1_6:50786629-50791321:+ TFAP2B Out-of-Frame Out-of-Frame GN=TFAP2B Out-of-Frame E_20221201_NO30_400nL_HLAc1_2e7_directIP_titration_rep3_Slot2-4_1_3518 40mio-rep4 E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep4_Slot2-5_1_3522.6026.1513.2 2 1 1 12.56 8.27 1.83 4.28 84.5 7 1 1 6250.0 Hydrogen 27 (R) 9 ATARGSPSW (A) 9 KDGKnPSW A/T/A/R\G S|P|S\W 77.8 7 4 5 2 0 unbiased 20.817263 0.805254 466.7294 932.458 -0.0077 -8.3 -3.6000000000000005 9.79 9562.7 nuORFdb_human ENST00000393655.3_1_6:50786629-50791321:+ ENST00000393655.3_1_6:50786629-50791321:+ TFAP2B Out-of-Frame Out-of-Frame GN=TFAP2B Out-of-Frame E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep4_Slot2-5_1_3522 20mio-rep2 E_20221201_NO30_400nL_HLAc1_2e7_directIP_titration_rep3_Slot2-4_1_3518.23023.411.1 1 1 1 10.6 8.49 2.62 2.11 85.6 6 0 0 2210.0 Hydrogen 37 (K) 9 ATDLIPVSY (T) 9 TAnIIVmSL A T D\L|I|P\V\S-Y 66.7 6 5 2 2 1 b-ion bias 79.522049 1.478735 978.5115 978.514 -0.0032 -3.3 3.0 3.8 7957.9 nuORFdb_human ENST00000466470.5_1_1:203764858-203765578:+ ENST00000466470.5_1_1:203764858-203765578:+ ZC3H11A ncRNA Processed Transcript lncRNA GN=ZC3H11A ncRNA Processed Transcript E_20221201_NO30_400nL_HLAc1_2e7_directIP_titration_rep3_Slot2-4_1_3518 40mio-rep2 E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep2_Slot2-5_1_3520.22964.407.1 1 2 1 12.05 7.51 4.9 4.54 85.5 6 0 0 8660.0 Hydrogen 37 (K) 9 ATDLIPVSY (T) 9 LISAEVPSY A T D\L|I|P|V\S\Y 66.7 6 6 3 3 0 dual b/y bias 79.330208 1.481367 978.5101 978.514 -0.0042 -4.3 0.6000000000000005 3.8 7957.9 nuORFdb_human ENST00000466470.5_1_1:203764858-203765578:+ ENST00000466470.5_1_1:203764858-203765578:+ ZC3H11A ncRNA Processed Transcript lncRNA GN=ZC3H11A ncRNA Processed Transcript E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep2_Slot2-5_1_3520 40mio-rep3 E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep3_Slot2-5_1_3521.22973.406.1 1 1 1 10.6 6.76 5.21 3.84 85.7 6 0 0 5450.0 Hydrogen 37 (K) 9 ATDLIPVSY (T) 9 LISAEVPSY A T D\L\I|P|V\S\Y 66.7 6 6 2 2 0 b-ion bias 79.358711 1.481556 978.5129 978.514 -0.0012 -1.3 3.3 3.8 7957.9 nuORFdb_human ENST00000466470.5_1_1:203764858-203765578:+ ENST00000466470.5_1_1:203764858-203765578:+ ZC3H11A ncRNA Processed Transcript lncRNA GN=ZC3H11A ncRNA Processed Transcript E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep3_Slot2-5_1_3521 40mio-rep4 E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep4_Slot2-5_1_3522.22973.402.1 1 1 1 11.12 8.27 4.69 2.85 87.3 6 0 0 4600.0 Hydrogen 37 (K) 9 ATDLIPVSY (T) 9 LISAEVPSY A T D\L\I|P\V|S\Y 66.7 6 6 2 2 0 b-ion bias 79.346535 1.483878 978.5055 978.514 -0.0092 -9.4 -4.7 3.8 7957.9 nuORFdb_human ENST00000466470.5_1_1:203764858-203765578:+ ENST00000466470.5_1_1:203764858-203765578:+ ZC3H11A ncRNA Processed Transcript lncRNA GN=ZC3H11A ncRNA Processed Transcript E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep4_Slot2-5_1_3522 40mio-rep1 E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep1_Slot1-04_1_3496.12177.1469.2 2 1 1 12.56 9.21 5.15 3.35 77.6 8 1 1 7980.0 Hydrogen 63 (M) 10 ATGGGGWRSW (A) 10 ATGGGQEQSSL A/T/G/G/G/G/W/R\S W 80.0 8 1 7 0 0 y-ion bias 42.063869 0.832849 517.7428 1034.48 -0.0015 -1.5 1.7000000000000002 9.79 8445.5 nuORFdb_human ENST00000356166.10_3_16:30669855-30670089:+ ENST00000356166.10_3_16:30669855-30670089:+ FBRS 5' uORF 5' uORF GN=FBRS 5' uORF E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep1_Slot1-04_1_3496 40mio-rep2 E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep2_Slot2-5_1_3520.11881.1498.2 2 1 1 9.89 4.73 5.69 5.15 56.0 8 1 1 6650.0 Hydrogen 63 (M) 10 ATGGGGWRSW (A) 10 ATGGQQKEnT A/T/G/G|G/G/W|R|S W 80.0 8 3 8 3 0 y-ion bias 41.042141 0.814723 517.7391 1034.48 -0.0095 -9.2 -4.299999999999999 9.79 8445.5 nuORFdb_human ENST00000356166.10_3_16:30669855-30670089:+ ENST00000356166.10_3_16:30669855-30670089:+ FBRS 5' uORF 5' uORF GN=FBRS 5' uORF E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep2_Slot2-5_1_3520 10mio-rep2 E_20221201_NO30_400nL_HLAc1_1e7_directIP_titration_rep2_Slot2-3_1_3513.12000.821.1 1 1 1 7.96 3.65 3.26 4.31 79.1 6 0 0 2350.0 Hydrogen 10 (L) 8 ATGPGNVL (L) 8 AASNPGVL A/T/G/P G\N\V\L 75.0 6 3 3 0 0 unbiased 41.459038 1.232911 728.3855 728.394 -0.0077 -10.6 -3.8999999999999995 5.57 2472.7 nuORFdb_human ENST00000507573.1_1_3:128890512-128890590:- ENST00000507573.1_1_3:128890512-128890590:- CNBP ncRNA Retained Intron lncRNA GN=CNBP ncRNA Retained Intron E_20221201_NO30_400nL_HLAc1_1e7_directIP_titration_rep2_Slot2-3_1_3513 10mio-rep3 E_20221201_NO30_400nL_HLAc1_1e7_directIP_titration_rep3_Slot2-3_1_3514.11978.828.1 1 1 1 8.02 3.92 4.65 4.1 77.6 6 0 0 2520.0 Hydrogen 10 (L) 8 ATGPGNVL (L) 8 AASNPGVL A/T/G/P G\N\V\L 75.0 6 3 3 0 0 unbiased 41.383305 1.229216 728.3924 728.394 -0.0017 -2.4 3.9 5.57 2472.7 nuORFdb_human ENST00000507573.1_1_3:128890512-128890590:- ENST00000507573.1_1_3:128890512-128890590:- CNBP ncRNA Retained Intron lncRNA GN=CNBP ncRNA Retained Intron E_20221201_NO30_400nL_HLAc1_1e7_directIP_titration_rep3_Slot2-3_1_3514 20mio-rep3 E_20221201_NO30_400nL_HLAc1_2e7_directIP_titration_rep4_Slot2-4_1_3519.11944.814.1 1 1 1 7.67 3.55 4.88 4.12 77.3 6 0 0 2770.0 Hydrogen 10 (L) 8 ATGPGNVL (L) 8 ATGAPAQL A/T/G/P G\N\V\L 75.0 6 3 3 0 0 unbiased 41.260296 1.237278 728.3884 728.394 -0.0057 -7.9 -2.1000000000000005 5.57 2472.7 nuORFdb_human ENST00000507573.1_1_3:128890512-128890590:- ENST00000507573.1_1_3:128890512-128890590:- CNBP ncRNA Retained Intron lncRNA GN=CNBP ncRNA Retained Intron E_20221201_NO30_400nL_HLAc1_2e7_directIP_titration_rep4_Slot2-4_1_3519 40mio-rep1 E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep1_Slot1-04_1_3496.12222.805.1 1 1 1 7.11 3.83 3.55 3.28 74.5 6 0 0 3030.0 Hydrogen 10 (L) 8 ATGPGNVL (L) 8 AASNPGVL A/T/G/P G-N\V\L 75.0 6 2 3 0 1 unbiased 42.219238 1.243035 728.3913 728.394 -0.0027 -3.7 -0.5 5.57 2472.7 nuORFdb_human ENST00000507573.1_1_3:128890512-128890590:- ENST00000507573.1_1_3:128890512-128890590:- CNBP ncRNA Retained Intron lncRNA GN=CNBP ncRNA Retained Intron E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep1_Slot1-04_1_3496 20mio-rep2 E_20221201_NO30_400nL_HLAc1_2e7_directIP_titration_rep3_Slot2-4_1_3518.14871.1318.2 2 1 1 9.38 6.07 6.02 3.31 66.3 5 1 1 3490.0 Hydrogen 6 (F) 11 ATNPSTTRVIW (K) 11 ATNRPLVGVnGF A T/N|P S T\T R V|I\W 27.3 3 4 3 2 0 unbiased 51.365505 0.927564 623.3299 1245.659 -0.0059 -4.7 1.5999999999999996 9.79 2770.3 nuORFdb_human ENST00000552408.1_1_12:57144862-57144931:- ENST00000552408.1_1_12:57144862-57144931:- PRIM1 ncRNA Processed Transcript lncRNA GN=PRIM1 ncRNA Processed Transcript E_20221201_NO30_400nL_HLAc1_2e7_directIP_titration_rep3_Slot2-4_1_3518 40mio-rep1 E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep1_Slot1-04_1_3496.15014.1291.2 2 1 1 19.43 8.9 12.74 10.53 90.1 9 1 1 7920.0 Hydrogen 6 (F) 11 ATNPSTTRVIW (K) 11 ATGGTPRSLSAID A/T/N|P|S/T/T R\V|I\W 81.8 9 5 7 3 0 unbiased 51.862774 0.944425 623.332 1245.659 -0.0019 -1.5 1.7000000000000002 9.79 2770.3 nuORFdb_human ENST00000552408.1_1_12:57144862-57144931:- ENST00000552408.1_1_12:57144862-57144931:- PRIM1 ncRNA Processed Transcript lncRNA GN=PRIM1 ncRNA Processed Transcript E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep1_Slot1-04_1_3496 40mio-rep2 E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep2_Slot2-5_1_3520.14727.1296.2 2 1 1 13.8 6.55 9.06 7.25 78.8 8 1 1 7720.0 Hydrogen 6 (F) 11 ATNPSTTRVIW (K) 11 ATGGTPRSLSAID A/T/N|P/S/T T/R V|I\W 63.6 7 3 7 2 0 y-ion bias 50.873085 0.941296 623.3308 1245.659 -0.0039 -3.1 1.8000000000000003 9.79 2770.3 nuORFdb_human ENST00000552408.1_1_12:57144862-57144931:- ENST00000552408.1_1_12:57144862-57144931:- PRIM1 ncRNA Processed Transcript lncRNA GN=PRIM1 ncRNA Processed Transcript E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep2_Slot2-5_1_3520 40mio-rep3 E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep3_Slot2-5_1_3521.14739.1306.2 2 1 1 12.23 5.9 8.59 6.33 73.8 7 1 1 4910.0 Hydrogen 6 (F) 11 ATNPSTTRVIW (K) 11 ATGGTPRSLSAID A T/N|P/S/T/T R V|I\W 54.5 6 3 6 2 0 y-ion bias 50.917 0.935192 623.332 1245.659 -0.0019 -1.5 3.0999999999999996 9.79 2770.3 nuORFdb_human ENST00000552408.1_1_12:57144862-57144931:- ENST00000552408.1_1_12:57144862-57144931:- PRIM1 ncRNA Processed Transcript lncRNA GN=PRIM1 ncRNA Processed Transcript E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep3_Slot2-5_1_3521 40mio-rep4 E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep4_Slot2-5_1_3522.14730.1304.2 2 1 1 11.83 4.33 8.05 7.5 75.3 7 1 1 5460.0 Hydrogen 6 (F) 11 ATNPSTTRVIW (K) 11 ATNITALGLEGW A T/N/P/S|T/T R V|I\W 54.5 6 3 6 2 0 y-ion bias 50.871574 0.936613 623.3314 1245.659 -0.0039 -3.1 1.6 9.79 2770.3 nuORFdb_human ENST00000552408.1_1_12:57144862-57144931:- ENST00000552408.1_1_12:57144862-57144931:- PRIM1 ncRNA Processed Transcript lncRNA GN=PRIM1 ncRNA Processed Transcript E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep4_Slot2-5_1_3522 SCP E_20221201_NO30_400nL_HLAc1_4e7_PDAC_Slot2-6_1_3524.5843.1494.2 2 1 1 12.39 10.8 2.42 1.59 82.9 5 1 1 15900.0 Hydrogen 19 (S) 9 ATRTCGAVL (-) 9 VGMTSNGVAL A T R/T C\G\A|V\L 44.4 4 4 2 1 0 b-ion bias Carbamidomethylation C:Carbamidomethylation, 20.19277 0.817075 474.7467 948.493 -0.0064 -6.8 -4.4 9.79 2813.1 nuORFdb_human ENST00000262510.10_2_16:57051083-57054679:+ ENST00000262510.10_2_16:57051083-57054679:+ NLRC5 5' Overlap uORF 5' Overlap uORF GN=NLRC5 5' Overlap uORF E_20221201_NO30_400nL_HLAc1_4e7_PDAC_Slot2-6_1_3524 Exploris_FAIMS VH20221207_MKP_FAIMS2022E_HLAc1_4e7_PDAC.19401.19401.2 2 1 1 12.47 10.1 1.84 2.37 90.3 5 1 1 9250000.0 Hydrogen 19 (S) 9 ATRTCGAVL (-) 9 KSRDCAVL A T R\T C\G\A|V|L 44.4 4 5 2 2 0 b-ion bias Carbamidomethylation C:Carbamidomethylation, 0.992 94.2 41.08 31.93 -50.0 474.7484 948.493 -0.0036 -3.8 -1.1999999999999997 9.79 2813.1 nuORFdb_human ENST00000262510.10_2_16:57051083-57054679:+ ENST00000262510.10_2_16:57051083-57054679:+ NLRC5 5' Overlap uORF 5' Overlap uORF GN=NLRC5 5' Overlap uORF VH20221207_MKP_FAIMS2022E_HLAc1_4e7_PDAC 20mio-rep1 E_20221201_NO30_400nL_HLAc1_2e7_directIP_titration_rep1_Slot2-4_1_3515.17863.1443.2 2 1 1 9.12 4.69 4.86 4.42 61.7 7 0 0 5840.0 Hydrogen 9 (D) 9 ATVLIQHPF (-) 9 ASLLLTRHn A/T/V|L|I|Q/H-P F 77.8 7 3 6 3 1 dual b/y bias 61.691971 0.849081 513.2897 1025.578 -0.0051 -5.0 1.2000000000000002 6.78 1910.2 nuORFdb_human ENST00000467337.6_1_17:28808244-28811254:+ ENST00000467337.6_1_17:28808244-28811254:+ GOSR1 5' uORF 5' uORF GN=GOSR1 5' uORF E_20221201_NO30_400nL_HLAc1_2e7_directIP_titration_rep1_Slot2-4_1_3515 40mio-rep1 E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep1_Slot1-04_1_3496.18052.1410.2 2 2 1 12.2 5.56 6.64 6.64 76.9 7 0 0 14800.0 Hydrogen 9 (D) 9 ATVLIQHPF (-) 9 ALSLIHLCV A/T/V|L|I|Q/H|P F 77.8 7 4 7 4 0 dual b/y bias 62.358082 0.870122 513.2901 1025.578 -0.0051 -5.0 -1.7999999999999998 6.78 1910.2 nuORFdb_human ENST00000467337.6_1_17:28808244-28811254:+ ENST00000467337.6_1_17:28808244-28811254:+ GOSR1 5' uORF 5' uORF GN=GOSR1 5' uORF E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep1_Slot1-04_1_3496 40mio-rep4 E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep4_Slot2-5_1_3522.17799.1431.2 2 1 1 7.6 3.73 3.87 3.87 57.2 6 0 0 6420.0 Hydrogen 9 (D) 9 ATVLIQHPF (-) 9 LQNRELVPG A T/V|L|I|Q/H-P F 55.6 5 3 5 3 1 dual b/y bias 61.468441 0.857112 513.2897 1025.578 -0.0051 -5.0 -0.2999999999999998 6.78 1910.2 nuORFdb_human ENST00000467337.6_1_17:28808244-28811254:+ ENST00000467337.6_1_17:28808244-28811254:+ GOSR1 5' uORF 5' uORF GN=GOSR1 5' uORF E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep4_Slot2-5_1_3522 1mio-rep1 E_20221201_NO30_400nL_HLAc1_1e6_directIP_titration_rep1_Slot1-06_1_3498.14718.1508.2 2 1 1 8.74 3.49 4.11 5.25 76.9 6 1 1 2310.0 Hydrogen 10 (S) 9 AVAQLASRW (-) 9 GLVAAAAAGGIM A V/A/Q/L/A|S R\W 55.6 5 2 5 1 0 y-ion bias 50.835758 0.80871 501.2789 1001.553 -0.002 -2.0 2.9000000000000004 9.79 2117.3 nuORFdb_human ENST00000449733.7_1_9:124044725-124044779:+ ENST00000449733.7_1_9:124044725-124044779:+ GSN 5' Overlap uORF 5' Overlap uORF GN=GSN 5' Overlap uORF E_20221201_NO30_400nL_HLAc1_1e6_directIP_titration_rep1_Slot1-06_1_3498 10mio-rep1 E_20221201_NO30_400nL_HLAc1_1e7_directIP_titration_rep1_Slot2-3_1_3512.14433.1556.2 2 5 1 11.32 6.45 6.0 4.87 82.4 7 1 1 16100.0 Hydrogen 10 (S) 9 AVAQLASRW (-) 9 QQLLASRW A V/A/Q|L/A|S/R\W 77.8 7 3 6 2 0 y-ion bias 49.859718 0.777923 501.2783 1001.553 -0.004 -4.0 3.7 9.79 2117.3 nuORFdb_human ENST00000449733.7_1_9:124044725-124044779:+ ENST00000449733.7_1_9:124044725-124044779:+ GSN 5' Overlap uORF 5' Overlap uORF GN=GSN 5' Overlap uORF E_20221201_NO30_400nL_HLAc1_1e7_directIP_titration_rep1_Slot2-3_1_3512 10mio-rep2 E_20221201_NO30_400nL_HLAc1_1e7_directIP_titration_rep2_Slot2-3_1_3513.14384.1505.2 2 5 1 11.98 5.57 4.79 6.41 82.4 6 1 1 19400.0 Hydrogen 10 (S) 9 AVAQLASRW (-) 9 AVAAGWVAVAS A V/A/Q|L/A|S R\W 55.6 5 3 5 2 0 unbiased 49.690334 0.810346 501.2779 1001.553 -0.004 -4.0 2.7 9.79 2117.3 nuORFdb_human ENST00000449733.7_1_9:124044725-124044779:+ ENST00000449733.7_1_9:124044725-124044779:+ GSN 5' Overlap uORF 5' Overlap uORF GN=GSN 5' Overlap uORF E_20221201_NO30_400nL_HLAc1_1e7_directIP_titration_rep2_Slot2-3_1_3513 10mio-rep3 E_20221201_NO30_400nL_HLAc1_1e7_directIP_titration_rep3_Slot2-3_1_3514.14349.1512.2 2 5 1 14.2 6.17 7.06 8.04 86.4 8 1 1 20500.0 Hydrogen 10 (S) 9 AVAQLASRW (-) 9 AVAAGWVAVAS A/V/A|Q/L/A|S/R\W 100.0 9 3 7 2 0 y-ion bias 49.572273 0.805903 501.2761 1001.553 -0.008 -8.0 -1.7000000000000002 9.79 2117.3 nuORFdb_human ENST00000449733.7_1_9:124044725-124044779:+ ENST00000449733.7_1_9:124044725-124044779:+ GSN 5' Overlap uORF 5' Overlap uORF GN=GSN 5' Overlap uORF E_20221201_NO30_400nL_HLAc1_1e7_directIP_titration_rep3_Slot2-3_1_3514 20mio-rep1 E_20221201_NO30_400nL_HLAc1_2e7_directIP_titration_rep1_Slot2-4_1_3515.14315.1508.2 2 3 1 13.28 5.6 6.34 7.69 85.8 8 1 1 22100.0 Hydrogen 10 (S) 9 AVAQLASRW (-) 9 QQLLASRW A/V/A/Q|L/A|S/R\W 100.0 9 3 7 2 0 y-ion bias 49.441628 0.808302 501.277 1001.553 -0.006 -6.0 0.20000000000000018 9.79 2117.3 nuORFdb_human ENST00000449733.7_1_9:124044725-124044779:+ ENST00000449733.7_1_9:124044725-124044779:+ GSN 5' Overlap uORF 5' Overlap uORF GN=GSN 5' Overlap uORF E_20221201_NO30_400nL_HLAc1_2e7_directIP_titration_rep1_Slot2-4_1_3515 20mio-rep2 E_20221201_NO30_400nL_HLAc1_2e7_directIP_titration_rep3_Slot2-4_1_3518.14416.1498.2 2 5 1 13.36 7.06 4.87 6.3 76.1 7 1 1 26500.0 Hydrogen 10 (S) 9 AVAQLASRW (-) 9 QLAGLALGVC A/V/A|Q|L/A|S|R W 77.8 7 4 7 4 0 dual b/y bias 49.794914 0.814785 501.2769 1001.553 -0.006 -6.0 0.2999999999999998 9.79 2117.3 nuORFdb_human ENST00000449733.7_1_9:124044725-124044779:+ ENST00000449733.7_1_9:124044725-124044779:+ GSN 5' Overlap uORF 5' Overlap uORF GN=GSN 5' Overlap uORF E_20221201_NO30_400nL_HLAc1_2e7_directIP_titration_rep3_Slot2-4_1_3518 20mio-rep3 E_20221201_NO30_400nL_HLAc1_2e7_directIP_titration_rep4_Slot2-4_1_3519.14347.1504.2 2 3 1 15.06 6.79 8.01 8.27 86.4 8 1 1 24100.0 Hydrogen 10 (S) 9 AVAQLASRW (-) 9 QQLLASRW A/V/A/Q|L/A|S|R\W 100.0 9 4 7 3 0 dual b/y bias 49.555622 0.811079 501.2768 1001.553 -0.006 -6.0 -0.20000000000000018 9.79 2117.3 nuORFdb_human ENST00000449733.7_1_9:124044725-124044779:+ ENST00000449733.7_1_9:124044725-124044779:+ GSN 5' Overlap uORF 5' Overlap uORF GN=GSN 5' Overlap uORF E_20221201_NO30_400nL_HLAc1_2e7_directIP_titration_rep4_Slot2-4_1_3519 40mio-rep1 E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep1_Slot1-04_1_3496.14590.1481.2 2 1 1 16.94 7.77 8.85 9.17 91.1 8 1 1 40400.0 Hydrogen 10 (S) 9 AVAQLASRW (-) 9 AVAAGWVAVAS A/V/A/Q|L/A|S|R\W 100.0 9 4 7 3 0 dual b/y bias 50.398243 0.825652 501.2784 1001.553 -0.004 -4.0 -0.7999999999999998 9.79 2117.3 nuORFdb_human ENST00000449733.7_1_9:124044725-124044779:+ ENST00000449733.7_1_9:124044725-124044779:+ GSN 5' Overlap uORF 5' Overlap uORF GN=GSN 5' Overlap uORF E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep1_Slot1-04_1_3496 40mio-rep2 E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep2_Slot2-5_1_3520.14291.1490.2 2 1 1 15.39 8.22 7.48 7.17 91.1 8 1 1 43300.0 Hydrogen 10 (S) 9 AVAQLASRW (-) 9 AVAAGWVAVAS A/V/A/Q|L/A|S/R\W 100.0 9 3 7 2 0 y-ion bias 49.367012 0.819825 501.2775 1001.553 -0.006 -6.0 -1.0999999999999996 9.79 2117.3 nuORFdb_human ENST00000449733.7_1_9:124044725-124044779:+ ENST00000449733.7_1_9:124044725-124044779:+ GSN 5' Overlap uORF 5' Overlap uORF GN=GSN 5' Overlap uORF E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep2_Slot2-5_1_3520 40mio-rep3 E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep3_Slot2-5_1_3521.14304.1495.2 2 2 1 15.34 6.64 7.79 8.7 93.3 7 1 1 40100.0 Hydrogen 10 (S) 9 AVAQLASRW (-) 9 QQLLASRW A V/A/Q|L/A|S/R\W 77.8 7 3 6 2 0 y-ion bias 49.415104 0.816495 501.278 1001.553 -0.004 -4.0 0.5999999999999996 9.79 2117.3 nuORFdb_human ENST00000449733.7_1_9:124044725-124044779:+ ENST00000449733.7_1_9:124044725-124044779:+ GSN 5' Overlap uORF 5' Overlap uORF GN=GSN 5' Overlap uORF E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep3_Slot2-5_1_3521 40mio-rep4 E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep4_Slot2-5_1_3522.14291.1492.2 2 1 1 14.05 6.9 6.77 7.16 85.5 8 1 1 19500.0 Hydrogen 10 (S) 9 AVAQLASRW (-) 9 AVAAGWVAVAS A/V/A|Q|L/A|S/R\W 100.0 9 4 7 3 0 dual b/y bias 49.356144 0.818407 501.2774 1001.553 -0.006 -6.0 -1.2999999999999998 9.79 2117.3 nuORFdb_human ENST00000449733.7_1_9:124044725-124044779:+ ENST00000449733.7_1_9:124044725-124044779:+ GSN 5' Overlap uORF 5' Overlap uORF GN=GSN 5' Overlap uORF E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep4_Slot2-5_1_3522 5mio-rep1 E_20221201_NO30_400nL_HLAc1_5e6_directIP_titration_rep1_Slot1-37_1_3502.14678.1505.2 2 4 1 11.06 5.07 5.57 5.99 86.3 8 1 1 13500.0 Hydrogen 10 (S) 9 AVAQLASRW (-) 9 QQLLASRW A/V/A/Q|L/A/S|R\W 100.0 9 3 7 2 0 y-ion bias 50.701519 0.81063 501.276 1001.553 -0.008 -8.0 -1.0 9.79 2117.3 nuORFdb_human ENST00000449733.7_1_9:124044725-124044779:+ ENST00000449733.7_1_9:124044725-124044779:+ GSN 5' Overlap uORF 5' Overlap uORF GN=GSN 5' Overlap uORF E_20221201_NO30_400nL_HLAc1_5e6_directIP_titration_rep1_Slot1-37_1_3502 5mio-rep2 E_20221201_NO30_400nL_HLAc1_5e6_directIP_titration_rep2_Slot2-2_1_3510.14586.1518.2 2 1 1 8.46 3.03 5.16 5.43 72.3 6 1 1 3470.0 Hydrogen 10 (S) 9 AVAQLASRW (-) 9 QQLLASRW A V/A/Q|L/A|S R\W 55.6 5 3 5 2 0 unbiased 50.37941 0.802434 501.2745 1001.553 -0.012 -11.9 -3.200000000000001 9.79 2117.3 nuORFdb_human ENST00000449733.7_1_9:124044725-124044779:+ ENST00000449733.7_1_9:124044725-124044779:+ GSN 5' Overlap uORF 5' Overlap uORF GN=GSN 5' Overlap uORF E_20221201_NO30_400nL_HLAc1_5e6_directIP_titration_rep2_Slot2-2_1_3510 5mio-rep3 E_20221201_NO30_400nL_HLAc1_5e6_directIP_titration_rep3_Slot2-2_1_3511.14609.1514.2 2 2 1 9.41 3.47 4.22 5.94 79.7 6 1 1 6270.0 Hydrogen 10 (S) 9 AVAQLASRW (-) 9 QQLLASRW A V/A/Q/L/A/S R\W 55.6 5 1 5 0 0 y-ion bias 50.464268 0.804907 501.2763 1001.553 -0.008 -8.0 0.1999999999999993 9.79 2117.3 nuORFdb_human ENST00000449733.7_1_9:124044725-124044779:+ ENST00000449733.7_1_9:124044725-124044779:+ GSN 5' Overlap uORF 5' Overlap uORF GN=GSN 5' Overlap uORF E_20221201_NO30_400nL_HLAc1_5e6_directIP_titration_rep3_Slot2-2_1_3511 20mio-rep1 E_20221201_NO30_400nL_HLAc1_2e7_directIP_titration_rep1_Slot2-4_1_3515.20711.661.1 1 2 1 8.71 4.94 2.32 3.77 87.6 7 0 0 4870.0 Hydrogen 14 (A) 9 AVPFPGPGV (A) 9 IGSALPWP A/V/P/F/P|G|P-G V 77.8 7 2 6 2 1 y-ion bias 71.5233 1.329524 840.4578 840.461 -0.0034 -4.1 2.1000000000000005 5.57 10996.3 nuORFdb_human ENST00000378827.4_1_20:6748921-6749233:+ ENST00000378827.4_1_20:6748921-6749233:+ BMP2 5' uORF 5' uORF GN=BMP2 5' uORF E_20221201_NO30_400nL_HLAc1_2e7_directIP_titration_rep1_Slot2-4_1_3515 20mio-rep2 E_20221201_NO30_400nL_HLAc1_2e7_directIP_titration_rep3_Slot2-4_1_3518.20755.652.1 1 1 1 12.74 5.87 4.72 6.87 89.4 7 0 0 2730.0 Hydrogen 14 (A) 9 AVPFPGPGV (A) 9 FLPGGPPVG A V/P|F|P|G|P\G-V 77.8 7 5 5 4 1 dual b/y bias 71.692131 1.335386 840.4567 840.461 -0.0044 -5.3 1.0 5.57 10996.3 nuORFdb_human ENST00000378827.4_1_20:6748921-6749233:+ ENST00000378827.4_1_20:6748921-6749233:+ BMP2 5' uORF 5' uORF GN=BMP2 5' uORF E_20221201_NO30_400nL_HLAc1_2e7_directIP_titration_rep3_Slot2-4_1_3518 40mio-rep1 E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep1_Slot1-04_1_3496.20890.634.1 1 4 1 9.73 5.53 2.77 4.21 85.1 7 0 0 16000.0 Hydrogen 14 (A) 9 AVPFPGPGV (A) 9 IGSALPWP A V/P/F/P|G|P\G-V 77.8 7 3 5 2 1 unbiased 72.164436 1.346058 840.4607 840.461 -0.0004 -0.5 2.7 5.57 10996.3 nuORFdb_human ENST00000378827.4_1_20:6748921-6749233:+ ENST00000378827.4_1_20:6748921-6749233:+ BMP2 5' uORF 5' uORF GN=BMP2 5' uORF E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep1_Slot1-04_1_3496 40mio-rep3 E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep3_Slot2-5_1_3521.18465.659.1 1 1 1 8.91 7.46 1.64 1.45 76.7 5 0 0 4140.0 Hydrogen 37 (F) 9 AVPSTLPGV (-) 9 TISPGIPGV A V/P S\T|L|P\G-V 55.6 5 4 3 2 1 unbiased 63.786251 1.330801 840.4815 840.482 -0.0005 -0.6 3.9999999999999996 5.57 4834.4 nuORFdb_human ENST00000230771.7_1_5:140071037-140071172:+ ENST00000230771.7_1_5:140071037-140071172:+ HARS2 5' uORF 5' uORF GN=HARS2 5' uORF E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep3_Slot2-5_1_3521 1mio-rep1 E_20221201_NO30_400nL_HLAc1_1e6_directIP_titration_rep1_Slot1-06_1_3498.17381.1397.2 2 1 1 9.09 4.18 5.47 4.91 68.6 8 1 1 1290.0 Hydrogen 12 (T) 10 AVSNLVSRAW (-) 10 KDGALVQPKm A V/S/N/L|V|S/R\A\W 80.0 8 4 6 2 0 unbiased 60.029133 0.878538 551.7985 1102.6 -0.0096 -8.7 -3.799999999999999 9.79 2415.9 nuORFdb_human ENST00000343677.3_1_6:26056391-26056457:- ENST00000343677.3_1_6:26056391-26056457:- HIST1H1C Out-of-Frame Out-of-Frame GN=HIST1H1C Out-of-Frame E_20221201_NO30_400nL_HLAc1_1e6_directIP_titration_rep1_Slot1-06_1_3498 10mio-rep1 E_20221201_NO30_400nL_HLAc1_1e7_directIP_titration_rep1_Slot2-3_1_3512.17159.1417.2 2 1 1 17.52 6.96 10.5 10.56 86.6 9 1 1 5660.0 Hydrogen 12 (T) 10 AVSNLVSRAW (-) 10 KDGALVQPKm A/V/S|N|L|V|S/R|A\W 100.0 10 6 8 5 0 dual b/y bias 59.273846 0.865433 551.7996 1102.6 -0.0076 -6.9 0.7999999999999998 9.79 2415.9 nuORFdb_human ENST00000343677.3_1_6:26056391-26056457:- ENST00000343677.3_1_6:26056391-26056457:- HIST1H1C Out-of-Frame Out-of-Frame GN=HIST1H1C Out-of-Frame E_20221201_NO30_400nL_HLAc1_1e7_directIP_titration_rep1_Slot2-3_1_3512 10mio-rep2 E_20221201_NO30_400nL_HLAc1_1e7_directIP_titration_rep2_Slot2-3_1_3513.17104.1413.2 2 2 1 16.63 8.52 8.51 8.11 89.3 9 1 1 11700.0 Hydrogen 12 (T) 10 AVSNLVSRAW (-) 10 KnQITALGAW A/V/S/N|L|V|S/R\A\W 100.0 10 5 7 3 0 unbiased 59.082699 0.868238 551.8005 1102.6 -0.0056 -5.1 1.6000000000000005 9.79 2415.9 nuORFdb_human ENST00000343677.3_1_6:26056391-26056457:- ENST00000343677.3_1_6:26056391-26056457:- HIST1H1C Out-of-Frame Out-of-Frame GN=HIST1H1C Out-of-Frame E_20221201_NO30_400nL_HLAc1_1e7_directIP_titration_rep2_Slot2-3_1_3513 10mio-rep3 E_20221201_NO30_400nL_HLAc1_1e7_directIP_titration_rep3_Slot2-3_1_3514.17060.1416.2 2 1 1 14.71 7.57 5.99 7.14 82.6 8 1 1 5160.0 Hydrogen 12 (T) 10 AVSNLVSRAW (-) 10 KDGALVQPKm A V/S/N|L|V|S|R\A\W 80.0 8 6 6 4 0 dual b/y bias 58.931129 0.866436 551.8011 1102.6 -0.0056 -5.1 1.2000000000000002 9.79 2415.9 nuORFdb_human ENST00000343677.3_1_6:26056391-26056457:- ENST00000343677.3_1_6:26056391-26056457:- HIST1H1C Out-of-Frame Out-of-Frame GN=HIST1H1C Out-of-Frame E_20221201_NO30_400nL_HLAc1_1e7_directIP_titration_rep3_Slot2-3_1_3514 20mio-rep1 E_20221201_NO30_400nL_HLAc1_2e7_directIP_titration_rep1_Slot2-4_1_3515.17077.1401.2 2 2 1 13.72 5.48 8.24 8.24 80.5 8 1 1 19200.0 Hydrogen 12 (T) 10 AVSNLVSRAW (-) 10 KDGALVQPKm A V/S/N/L|V|S|R|A\W 80.0 8 5 7 4 0 dual b/y bias 58.975945 0.875754 551.7999 1102.6 -0.0076 -6.9 -0.7000000000000002 9.79 2415.9 nuORFdb_human ENST00000343677.3_1_6:26056391-26056457:- ENST00000343677.3_1_6:26056391-26056457:- HIST1H1C Out-of-Frame Out-of-Frame GN=HIST1H1C Out-of-Frame E_20221201_NO30_400nL_HLAc1_2e7_directIP_titration_rep1_Slot2-4_1_3515 20mio-rep2 E_20221201_NO30_400nL_HLAc1_2e7_directIP_titration_rep3_Slot2-4_1_3518.17160.1399.2 2 1 1 15.63 7.61 8.44 8.02 86.7 8 1 1 5150.0 Hydrogen 12 (T) 10 AVSNLVSRAW (-) 10 KDGALVQPKm A V/S/N|L|V|S/R|A\W 80.0 8 5 7 4 0 dual b/y bias 59.273724 0.877267 551.7984 1102.6 -0.0116 -10.6 -4.3 9.79 2415.9 nuORFdb_human ENST00000343677.3_1_6:26056391-26056457:- ENST00000343677.3_1_6:26056391-26056457:- HIST1H1C Out-of-Frame Out-of-Frame GN=HIST1H1C Out-of-Frame E_20221201_NO30_400nL_HLAc1_2e7_directIP_titration_rep3_Slot2-4_1_3518 20mio-rep3 E_20221201_NO30_400nL_HLAc1_2e7_directIP_titration_rep4_Slot2-4_1_3519.17128.1400.2 2 2 1 15.83 6.14 7.79 9.68 87.4 9 1 1 14900.0 Hydrogen 12 (T) 10 AVSNLVSRAW (-) 10 KDGALVQPKm A/V/S/N|L|V|S/R|A\W 100.0 10 5 8 4 0 dual b/y bias 59.159725 0.876174 551.8022 1102.6 -0.0036 -3.3 2.5 9.79 2415.9 nuORFdb_human ENST00000343677.3_1_6:26056391-26056457:- ENST00000343677.3_1_6:26056391-26056457:- HIST1H1C Out-of-Frame Out-of-Frame GN=HIST1H1C Out-of-Frame E_20221201_NO30_400nL_HLAc1_2e7_directIP_titration_rep4_Slot2-4_1_3519 40mio-rep1 E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep1_Slot1-04_1_3496.17251.1374.2 2 5 1 17.07 6.98 12.03 10.08 87.1 8 1 1 32200.0 Hydrogen 12 (T) 10 AVSNLVSRAW (-) 10 KDGALVQPKm A V/S/N|L|V|S|R\A\W 80.0 8 6 6 4 0 dual b/y bias 59.589619 0.892371 551.804 1102.6 0.0004 0.3 3.5 9.79 2415.9 nuORFdb_human ENST00000343677.3_1_6:26056391-26056457:- ENST00000343677.3_1_6:26056391-26056457:- HIST1H1C Out-of-Frame Out-of-Frame GN=HIST1H1C Out-of-Frame E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep1_Slot1-04_1_3496 40mio-rep2 E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep2_Slot2-5_1_3520.17187.1385.2 2 1 1 15.23 7.32 9.17 7.91 84.9 8 1 1 6970.0 Hydrogen 12 (T) 10 AVSNLVSRAW (-) 10 KDGALVQPKm A V/S/N|L|V|S/R|A\W 80.0 8 5 7 4 0 dual b/y bias 59.36937 0.885903 551.8012 1102.6 -0.0056 -5.1 -0.1999999999999993 9.79 2415.9 nuORFdb_human ENST00000343677.3_1_6:26056391-26056457:- ENST00000343677.3_1_6:26056391-26056457:- HIST1H1C Out-of-Frame Out-of-Frame GN=HIST1H1C Out-of-Frame E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep2_Slot2-5_1_3520 40mio-rep3 E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep3_Slot2-5_1_3521.17043.1387.2 2 3 1 15.55 5.32 10.6 10.23 83.2 8 1 1 30300.0 Hydrogen 12 (T) 10 AVSNLVSRAW (-) 10 KnQITALGAW A V/S/N|L|V|S/R|A\W 80.0 8 5 7 4 0 dual b/y bias 58.873043 0.884478 551.8012 1102.6 -0.0056 -5.1 -0.5 9.79 2415.9 nuORFdb_human ENST00000343677.3_1_6:26056391-26056457:- ENST00000343677.3_1_6:26056391-26056457:- HIST1H1C Out-of-Frame Out-of-Frame GN=HIST1H1C Out-of-Frame E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep3_Slot2-5_1_3521 40mio-rep4 E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep4_Slot2-5_1_3522.17210.1384.2 2 2 1 14.04 6.76 9.36 7.28 81.4 9 1 1 25600.0 Hydrogen 12 (T) 10 AVSNLVSRAW (-) 10 KnQITALGAW A/V/S/N/L|V|S/R\A\W 100.0 10 4 7 2 0 unbiased 59.434322 0.886524 551.8018 1102.6 -0.0036 -3.3 1.4000000000000004 9.79 2415.9 nuORFdb_human ENST00000343677.3_1_6:26056391-26056457:- ENST00000343677.3_1_6:26056391-26056457:- HIST1H1C Out-of-Frame Out-of-Frame GN=HIST1H1C Out-of-Frame E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep4_Slot2-5_1_3522 5mio-rep1 E_20221201_NO30_400nL_HLAc1_5e6_directIP_titration_rep1_Slot1-37_1_3502.17398.1409.2 2 2 1 13.79 6.46 7.22 7.34 85.3 8 1 1 10300.0 Hydrogen 12 (T) 10 AVSNLVSRAW (-) 10 INSVAVICGGI A V/S/N/L|V|S/R|A\W 80.0 8 4 7 3 0 unbiased 60.095108 0.87087 551.7983 1102.6 -0.0116 -10.6 -3.5999999999999996 9.79 2415.9 nuORFdb_human ENST00000343677.3_1_6:26056391-26056457:- ENST00000343677.3_1_6:26056391-26056457:- HIST1H1C Out-of-Frame Out-of-Frame GN=HIST1H1C Out-of-Frame E_20221201_NO30_400nL_HLAc1_5e6_directIP_titration_rep1_Slot1-37_1_3502 5mio-rep3 E_20221201_NO30_400nL_HLAc1_5e6_directIP_titration_rep3_Slot2-2_1_3511.17260.1420.2 2 1 1 9.93 3.86 7.49 6.07 60.9 9 1 1 1900.0 Hydrogen 12 (T) 10 AVSNLVSRAW (-) 10 KDGALVQPKm A/V/S/N|L|V/S|R\A\W 100.0 10 5 7 3 0 unbiased 59.61821 0.864059 551.8002 1102.6 -0.0076 -6.9 1.299999999999999 9.79 2415.9 nuORFdb_human ENST00000343677.3_1_6:26056391-26056457:- ENST00000343677.3_1_6:26056391-26056457:- HIST1H1C Out-of-Frame Out-of-Frame GN=HIST1H1C Out-of-Frame E_20221201_NO30_400nL_HLAc1_5e6_directIP_titration_rep3_Slot2-2_1_3511 SCP E_20221201_NO30_400nL_HLAc1_4e7_PDAC_Slot2-6_1_3524.30197.1247.2 2 1 1 20.02 4.88 13.53 15.14 94.2 9 0 0 22300.0 Hydrogen 60 (K) 11 AWFDPLTLHLF (L) 11 AKNATQILnEKE A W|F|D|P|L|T/L|H/L\F 81.8 9 7 8 6 0 dual b/y bias 104.328728 0.971945 680.3574 1359.71 -0.0029 -2.1 0.2999999999999998 5.08 8698.6 nuORFdb_human TCONS_00006403_3:5735188-5735416:- TCONS_00006403_3:5735188-5735416:- linc-SUMF1-2 lincRNA lncRNA GN=linc-SUMF1-2 lincRNA E_20221201_NO30_400nL_HLAc1_4e7_PDAC_Slot2-6_1_3524 SCP E_20221201_NO30_400nL_HLAc1_4e7_PDAC_Slot2-6_1_3524.6477.1347.2 2 1 1 8.88 3.26 6.33 5.62 59.5 8 1 1 5280.0 Hydrogen 76 (R) 10 AYCGQCSERI (W) 10 AYCnLcLPGL A/Y/C|G/Q|C/S/E R\I 80.0 8 3 7 2 0 y-ion bias Carbamidomethylation C:Carbamidomethylation, 22.381023 0.909369 622.2609 1243.519 -0.0047 -3.8 -1.4 6.04 15158.4 nuORFdb_human ENST00000486681.5_3_1:2036198-2076794:+ ENST00000486681.5_3_1:2036198-2076794:+|ENST00000497183.5_3_1:2066738-2076851:+ PRKCZ 3' Overlap dORF 3' Overlap dORF GN=PRKCZ 3' Overlap dORF E_20221201_NO30_400nL_HLAc1_4e7_PDAC_Slot2-6_1_3524 SCP E_20221201_NO30_400nL_HLAc1_4e7_PDAC_Slot2-6_1_3524.9024.1612.3 3 1 1 7.91 2.07 5.74 5.84 57.2 6 1 1 17600.0 Hydrogen 2 (M) 9 AYHHYAFKI (-) 9 IKNPDSNKYA A/Y/H H/Y|A\F\K I 55.6 5 3 4 1 0 unbiased 31.175474 0.742743 383.8639 1149.584 -0.0065 -5.7 -3.3000000000000003 8.55 1280.5 nuORFdb_human ENST00000448876.5_1_3:185009426-185009459:+ ENST00000448876.5_1_3:185009426-185009459:+ MAP3K13 Out-of-Frame Out-of-Frame GN=MAP3K13 Out-of-Frame E_20221201_NO30_400nL_HLAc1_4e7_PDAC_Slot2-6_1_3524 SCP E_20221201_NO30_400nL_HLAc1_4e7_PDAC_Slot2-6_1_3524.24869.192.1 1 1 1 8.17 1.06 6.73 7.11 63.1 5 0 0 7210.0 Hydrogen 58 (G) 9 AYTDCVLYL (L) 9 LASLDCYIY A Y T D|C\V\L\Y\L 55.6 5 5 1 1 0 b-ion bias Carbamidomethylation C:Carbamidomethylation, 85.915352 1.607538 1117.5212 1117.523 -0.0034 -3.1 -0.7000000000000002 3.8 10202.5 nuORFdb_human ENST00000468046.1_1_9:136245909-136249825:+ ENST00000468046.1_1_9:136245909-136249825:+ STKLD1 ncRNA Processed Transcript lncRNA GN=STKLD1 ncRNA Processed Transcript E_20221201_NO30_400nL_HLAc1_4e7_PDAC_Slot2-6_1_3524 SCP E_20221201_NO30_400nL_HLAc1_4e7_PDAC_Slot2-6_1_3524.30011.195.1 1 1 1 15.34 5.34 10.12 10.0 93.7 7 0 0 23600.0 Hydrogen 68 (L) 9 CLYPFPAFL (G) 9 SVLFFPVMMG C L|Y|P|F|P-A|F\L 77.8 7 6 5 5 1 dual b/y bias Carbamidomethylation C:Carbamidomethylation, 103.686478 1.605947 1127.5587 1127.559 0.0006 0.5 2.9 5.52 10445.9 nuORFdb_human T098878_13:109918233-109918512:+ T098878_13:109918233-109918512:+ lincRNA lncRNA GN=nan lincRNA E_20221201_NO30_400nL_HLAc1_4e7_PDAC_Slot2-6_1_3524 Exploris_FAIMS VH20221207_MKP_FAIMS2022E_HLAc1_4e7_PDAC.37912.37912.2 2 1 1 10.89 7.4 3.3 3.48 68.2 7 0 0 3330000.0 Hydrogen 15 (M) 9 CPFEGGSSL (A) 9 SEPFCGGSI C P\F\E\G\G\S|S/L 77.8 7 6 2 1 0 b-ion bias Carbamidomethylation C:Carbamidomethylation, 1.0 85.5 70.57 79.72 -70.0 477.2038 953.403 -0.003 -3.2 -0.6000000000000001 4.0 2719.1 nuORFdb_human ENST00000270625.6_1_19:50000783-50001182:+ ENST00000270625.6_1_19:50000783-50001182:+ RPS11 Out-of-Frame Out-of-Frame GN=RPS11 Out-of-Frame VH20221207_MKP_FAIMS2022E_HLAc1_4e7_PDAC Exploris_FAIMS VH20221207_MKP_FAIMS2022E_HLAc1_4e7_PDAC.56971.56971.2 2 1 1 9.59 5.09 4.94 4.5 57.0 6 0 0 3210000.0 Hydrogen 15 (M) 11 CPFEGGSSLAW (-) 11 LPSSnCKSMW C P F\E\G G\S S\L/A/W 36.4 4 4 2 0 0 b-ion bias Carbamidomethylation C:Carbamidomethylation, 0.974 100.0 99.48 120.0 -70.0 605.7617 1210.52 -0.0036 -3.0 -0.3999999999999999 4.0 2719.1 nuORFdb_human ENST00000270625.6_1_19:50000783-50001182:+ ENST00000270625.6_1_19:50000783-50001182:+ RPS11 Out-of-Frame Out-of-Frame GN=RPS11 Out-of-Frame VH20221207_MKP_FAIMS2022E_HLAc1_4e7_PDAC SCP E_20221201_NO30_400nL_HLAc1_4e7_PDAC_Slot2-6_1_3524.6406.1559.2 2 3 1 11.87 7.92 3.91 3.95 93.2 5 0 0 21000.0 Hydrogen 33 (S) 9 CPHGGGVAL (A) 9 CGTKAmAL C P/H\G G G\V\A\L 44.4 4 4 1 0 0 b-ion bias Carbamidomethylation C:Carbamidomethylation, 22.135952 0.776377 434.2086 867.414 -0.0034 -3.9 -1.5 6.74 6052.8 nuORFdb_human ENST00000619697.1_1_17:38804565-38804739:+ ENST00000619697.1_1_17:38804565-38804739:+ RP11-458J1.1 Antisense lncRNA GN=RP11-458J1.1 Antisense E_20221201_NO30_400nL_HLAc1_4e7_PDAC_Slot2-6_1_3524 Exploris_FAIMS VH20221207_MKP_FAIMS2022E_HLAc1_4e7_PDAC.20570.20570.2 2 1 1 13.08 6.94 6.71 6.13 87.3 5 0 0 14200000.0 Hydrogen 33 (S) 9 CPHGGGVAL (A) 9 AHPCGAAAI C P H\G-G G\V|A|L 44.4 4 4 2 2 1 b-ion bias Carbamidomethylation C:Carbamidomethylation, 0.986 100.0 43.27 21.58 -50.0 434.2095 867.414 -0.0025 -2.8 -0.19999999999999973 6.74 6052.8 nuORFdb_human ENST00000619697.1_1_17:38804565-38804739:+ ENST00000619697.1_1_17:38804565-38804739:+ RP11-458J1.1 Antisense lncRNA GN=RP11-458J1.1 Antisense VH20221207_MKP_FAIMS2022E_HLAc1_4e7_PDAC SCP E_20221201_NO30_400nL_HLAc1_4e7_PDAC_Slot2-6_1_3524.7306.1416.2 2 1 1 15.73 3.39 13.41 12.34 85.7 8 1 1 61700.0 Hydrogen 21 (V) 9 DALKMSHGY (-) 9 GELDTPINY D/A/L/K/M|S|H\G\Y 100.0 9 4 6 2 0 unbiased 25.243755 0.866132 511.2411 1021.477 -0.0024 -2.4 0.0 6.74 2968.5 nuORFdb_human ENST00000619012.4_1_7:142560906-142560996:+ ENST00000619012.4_1_7:142560906-142560996:+ EPHB6 5' Overlap uORF 5' Overlap uORF GN=EPHB6 5' Overlap uORF E_20221201_NO30_400nL_HLAc1_4e7_PDAC_Slot2-6_1_3524 Exploris_FAIMS VH20221207_MKP_FAIMS2022E_HLAc1_4e7_PDAC.21600.21600.2 2 2 1 11.15 2.3 11.15 8.85 67.5 7 1 1 6840000.0 Hydrogen 21 (V) 9 DALKMSHGY (-) 9 DAIKQYmH D A|L/K/M/S/H|G/Y 77.8 7 2 7 2 0 y-ion bias 0.938 100.0 45.08 120.0 -50.0 511.2394 1021.477 -0.0056 -5.5 -2.9 6.74 2968.5 nuORFdb_human ENST00000619012.4_1_7:142560906-142560996:+ ENST00000619012.4_1_7:142560906-142560996:+ EPHB6 5' Overlap uORF 5' Overlap uORF GN=EPHB6 5' Overlap uORF VH20221207_MKP_FAIMS2022E_HLAc1_4e7_PDAC SCP E_20221201_NO30_400nL_HLAc1_4e7_PDAC_Slot2-6_1_3524.4086.1439.2 2 2 1 11.02 8.21 11.02 69.0 6 1 1 15800.0 M25m Hydrogen 21 (V) 9 DALKmSHGY (-) 9 D A/L/K/m S|H\G\Y 55.6 5 3 4 1 0 unbiased m:Oxidized methionine 14.127112 0.851705 519.2379 1021.477 15.9916 -3.2 -0.8000000000000003 6.74 2968.5 nuORFdb_human ENST00000619012.4_1_7:142560906-142560996:+ ENST00000619012.4_1_7:142560906-142560996:+ EPHB6 5' Overlap uORF 5' Overlap uORF GN=EPHB6 5' Overlap uORF E_20221201_NO30_400nL_HLAc1_4e7_PDAC_Slot2-6_1_3524 Exploris_FAIMS VH20221207_MKP_FAIMS2022E_HLAc1_4e7_PDAC.14314.14314.2 2 2 1 17.69 3.68 12.09 14.01 86.8 7 1 1 8620000.0 M25m Hydrogen 21 (V) 9 DALKmSHGY (-) 9 HVKnATmGY D A|L/K/m/S/H|G|Y 77.8 7 3 7 3 0 dual b/y bias m:Oxidized methionine 0.997 93.3 30.8 120.0 -50.0 519.2387 1021.477 15.993 -1.8 0.8 6.74 2968.5 nuORFdb_human ENST00000619012.4_1_7:142560906-142560996:+ ENST00000619012.4_1_7:142560906-142560996:+ EPHB6 5' Overlap uORF 5' Overlap uORF GN=EPHB6 5' Overlap uORF VH20221207_MKP_FAIMS2022E_HLAc1_4e7_PDAC SCP E_20221201_NO30_400nL_HLAc1_4e7_PDAC_Slot2-6_1_3524.16723.275.1 1 2 1 13.51 11.67 5.41 1.84 91.4 7 1 1 16900.0 Hydrogen 2 (M) 9 DASKLTYIY (I) 9 DASLKTYLY D A/S/K\L\T\Y\I\Y 77.8 7 5 2 0 0 b-ion bias 57.77282 1.558843 1073.549 1073.551 -0.0014 -1.3 1.0999999999999999 5.83 2053.4 nuORFdb_human ENST00000383746.7_2_3:44396236-44396287:+ ENST00000383746.7_2_3:44396236-44396287:+ TCAIM 5' Overlap uORF 5' Overlap uORF GN=TCAIM 5' Overlap uORF E_20221201_NO30_400nL_HLAc1_4e7_PDAC_Slot2-6_1_3524 20mio-rep3 E_20221201_NO30_400nL_HLAc1_2e7_directIP_titration_rep4_Slot2-4_1_3519.17909.1465.2 2 1 1 8.0 3.2 5.94 4.8 62.8 6 1 1 2380.0 Hydrogen 56 (E) 9 DEAAAWRSW (R) 9 EEGAAASISQE D E|A|A|A|W/R S\W 55.6 5 5 5 4 0 dual b/y bias 61.85564 0.835369 546.2435 1091.491 -0.0098 -8.9 -3.1000000000000005 4.37 8811.4 nuORFdb_human ENST00000371142.8_1_10:98336578-98346758:- ENST00000371142.8_1_10:98336578-98346758:- TM9SF3 5' Overlap uORF 5' Overlap uORF GN=TM9SF3 5' Overlap uORF E_20221201_NO30_400nL_HLAc1_2e7_directIP_titration_rep4_Slot2-4_1_3519 40mio-rep1 E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep1_Slot1-04_1_3496.18156.1436.2 2 1 1 17.17 10.55 10.24 6.62 93.9 8 1 1 9360.0 Hydrogen 56 (E) 9 DEAAAWRSW (R) 9 DEAAAAEMQR D/E|A|A|A|W|R|S\W 100.0 9 7 7 6 0 dual b/y bias 62.717556 0.853467 546.2461 1091.491 -0.0058 -5.3 -2.0999999999999996 4.37 8811.4 nuORFdb_human ENST00000371142.8_1_10:98336578-98346758:- ENST00000371142.8_1_10:98336578-98346758:- TM9SF3 5' Overlap uORF 5' Overlap uORF GN=TM9SF3 5' Overlap uORF E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep1_Slot1-04_1_3496 40mio-rep2 E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep2_Slot2-5_1_3520.17785.1450.2 2 1 1 17.22 10.52 10.4 6.7 95.0 8 1 1 8750.0 Hydrogen 56 (E) 9 DEAAAWRSW (R) 9 DEAAAAEMQR D/E|A|A|A|W|R|S\W 100.0 9 7 7 6 0 dual b/y bias 61.437603 0.844836 546.2467 1091.491 -0.0038 -3.4 1.5000000000000004 4.37 8811.4 nuORFdb_human ENST00000371142.8_1_10:98336578-98346758:- ENST00000371142.8_1_10:98336578-98346758:- TM9SF3 5' Overlap uORF 5' Overlap uORF GN=TM9SF3 5' Overlap uORF E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep2_Slot2-5_1_3520 40mio-rep3 E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep3_Slot2-5_1_3521.17830.1454.2 2 2 1 13.58 7.04 8.13 6.54 84.5 6 1 1 17400.0 Hydrogen 56 (E) 9 DEAAAWRSW (R) 9 DEAAAAEMQR D E|A|A|A|W|R|S W 55.6 5 6 6 6 0 dual b/y bias 61.59354 0.842652 546.2471 1091.491 -0.0038 -3.4 1.1999999999999997 4.37 8811.4 nuORFdb_human ENST00000371142.8_1_10:98336578-98346758:- ENST00000371142.8_1_10:98336578-98346758:- TM9SF3 5' Overlap uORF 5' Overlap uORF GN=TM9SF3 5' Overlap uORF E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep3_Slot2-5_1_3521 40mio-rep4 E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep4_Slot2-5_1_3522.17781.1451.2 2 1 1 15.53 9.34 9.79 6.19 92.0 7 1 1 6840.0 Hydrogen 56 (E) 9 DEAAAWRSW (R) 9 DEAAAAEMQR D E|A|A|A|W|R|S\W 77.8 7 7 6 6 0 dual b/y bias 61.406292 0.844048 546.2452 1091.491 -0.0078 -7.1 -2.3999999999999995 4.37 8811.4 nuORFdb_human ENST00000371142.8_1_10:98336578-98346758:- ENST00000371142.8_1_10:98336578-98346758:- TM9SF3 5' Overlap uORF 5' Overlap uORF GN=TM9SF3 5' Overlap uORF E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep4_Slot2-5_1_3522 40mio-rep4 E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep4_Slot2-5_1_3522.7636.1643.3 3 1 1 7.26 4.14 3.5 3.12 64.4 5 2 3 70800.0 Hydrogen 8 (R) 10 DEFGDSRRRW (R) 10 nEnGNFKIVTDA D/E/F/G D|S R R R\W 40.0 4 2 4 1 0 y-ion bias 26.375484 0.723167 441.8751 1323.619 -0.0084 -6.4 -1.7000000000000002 6.12 4482.2 nuORFdb_human ENST00000375457.2_2_13:113862417-113862534:- ENST00000375457.2_2_13:113862417-113862534:- PCID2 5' uORF 5' uORF GN=PCID2 5' uORF E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep4_Slot2-5_1_3522 20mio-rep1 E_20221201_NO30_400nL_HLAc1_2e7_directIP_titration_rep1_Slot2-4_1_3515.19218.1403.2 2 2 1 11.22 2.29 9.14 8.93 78.7 6 1 1 6340.0 Hydrogen 12 (L) 9 DEFQLVSRY (I) 9 DEFQEVKYV D E/F|Q|L|V|S R\Y 55.6 5 5 5 4 0 dual b/y bias 66.369133 0.874617 578.7827 1156.563 -0.0046 -4.0 2.2 4.37 3102.7 nuORFdb_human ENST00000613993.1_2_6:106764001-106764076:- ENST00000613993.1_2_6:106764001-106764076:- ATG5 Out-of-Frame Out-of-Frame GN=ATG5 Out-of-Frame E_20221201_NO30_400nL_HLAc1_2e7_directIP_titration_rep1_Slot2-4_1_3515 40mio-rep1 E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep1_Slot1-04_1_3496.21291.1325.2 2 1 1 12.28 1.82 8.09 10.47 71.0 8 1 1 7300.0 Hydrogen 196 (S) 10 DLDYMGNLKY (G) 10 DLnYDTPFVF D/L|D|Y|M\G N/L|K-Y 80.0 8 5 6 4 1 dual b/y bias 73.549606 0.923009 616.283 1231.566 -0.0076 -6.2 -3.0 4.21 26630.8 nuORFdb_human ENST00000554084.5_1_14:100819360-100828234:- ENST00000554084.5_1_14:100819360-100828234:- WARS 3' Overlap dORF 3' Overlap dORF GN=WARS 3' Overlap dORF E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep1_Slot1-04_1_3496 20mio-rep2 E_20221201_NO30_400nL_HLAc1_2e7_directIP_titration_rep3_Slot2-4_1_3518.16608.1362.2 2 3 1 10.8 3.02 10.8 7.78 69.9 7 1 1 13900.0 M200m Hydrogen 196 (S) 10 DLDYmGNLKY (G) 10 VEDmYLVDFT D L/D|Y/m\G N/L|K\Y 60.0 6 4 5 2 0 unbiased m:Oxidized methionine 57.362724 0.900227 624.2812 1231.566 15.9884 -5.2 1.0999999999999996 4.21 26630.8 nuORFdb_human ENST00000554084.5_1_14:100819360-100828234:- ENST00000554084.5_1_14:100819360-100828234:- WARS 3' Overlap dORF 3' Overlap dORF GN=WARS 3' Overlap dORF E_20221201_NO30_400nL_HLAc1_2e7_directIP_titration_rep3_Slot2-4_1_3518 20mio-rep3 E_20221201_NO30_400nL_HLAc1_2e7_directIP_titration_rep4_Slot2-4_1_3519.16567.1357.2 2 2 1 14.56 7.04 11.18 7.53 80.4 7 1 1 7500.0 M200m Hydrogen 196 (S) 10 DLDYmGNLKY (G) 10 InnDHENVKY D L/D|Y/m|G N|L|K\Y 60.0 6 5 6 4 0 dual b/y bias m:Oxidized methionine 57.223118 0.903034 624.2796 1231.566 15.9864 -6.8 -1.0 4.21 26630.8 nuORFdb_human ENST00000554084.5_1_14:100819360-100828234:- ENST00000554084.5_1_14:100819360-100828234:- WARS 3' Overlap dORF 3' Overlap dORF GN=WARS 3' Overlap dORF E_20221201_NO30_400nL_HLAc1_2e7_directIP_titration_rep4_Slot2-4_1_3519 40mio-rep1 E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep1_Slot1-04_1_3496.16754.1320.2 2 2 1 14.8 6.08 9.6 8.72 76.3 7 1 1 6680.0 M200m Hydrogen 196 (S) 10 DLDYmGNLKY (G) 10 InnDHENVKY D/L|D|Y|m|G N L|K\Y 70.0 7 6 6 5 0 dual b/y bias m:Oxidized methionine 57.873074 0.926298 624.2826 1231.566 15.9924 -2.0 1.2000000000000002 4.21 26630.8 nuORFdb_human ENST00000554084.5_1_14:100819360-100828234:- ENST00000554084.5_1_14:100819360-100828234:- WARS 3' Overlap dORF 3' Overlap dORF GN=WARS 3' Overlap dORF E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep1_Slot1-04_1_3496 40mio-rep2 E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep2_Slot2-5_1_3520.16419.1344.2 2 2 1 15.39 4.56 15.39 10.83 77.8 7 1 1 17000.0 M200m Hydrogen 196 (S) 10 DLDYmGNLKY (G) 10 InnDHENVKY D L/D|Y|m|G N|L|K\Y 60.0 6 6 6 5 0 dual b/y bias m:Oxidized methionine 56.717434 0.911362 624.2804 1231.566 15.9864 -6.8 -1.8999999999999995 4.21 26630.8 nuORFdb_human ENST00000554084.5_1_14:100819360-100828234:- ENST00000554084.5_1_14:100819360-100828234:- WARS 3' Overlap dORF 3' Overlap dORF GN=WARS 3' Overlap dORF E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep2_Slot2-5_1_3520 40mio-rep3 E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep3_Slot2-5_1_3521.16430.1349.2 2 3 1 13.34 2.9 13.34 10.45 66.7 7 1 1 20700.0 M200m Hydrogen 196 (S) 10 DLDYmGNLKY (G) 10 InnDHENVKY D L|D|Y|m|G N|L\K\Y 60.0 6 7 5 5 0 dual b/y bias m:Oxidized methionine 56.756092 0.908029 624.2795 1231.566 15.9844 -8.5 -3.9000000000000004 4.21 26630.8 nuORFdb_human ENST00000554084.5_1_14:100819360-100828234:- ENST00000554084.5_1_14:100819360-100828234:- WARS 3' Overlap dORF 3' Overlap dORF GN=WARS 3' Overlap dORF E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep3_Slot2-5_1_3521 40mio-rep4 E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep4_Slot2-5_1_3522.16471.1343.2 2 2 1 12.46 3.43 11.74 9.03 71.2 8 1 1 13500.0 M200m Hydrogen 196 (S) 10 DLDYmGNLKY (G) 10 InnDHENVKY D/L/D|Y/m/G N|L|K\Y 80.0 8 4 7 3 0 unbiased m:Oxidized methionine 56.882889 0.912217 624.2816 1231.566 15.9904 -3.6 1.1 4.21 26630.8 nuORFdb_human ENST00000554084.5_1_14:100819360-100828234:- ENST00000554084.5_1_14:100819360-100828234:- WARS 3' Overlap dORF 3' Overlap dORF GN=WARS 3' Overlap dORF E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep4_Slot2-5_1_3522 Exploris AL20221201_MKP_PDAC_01.66316.66316.2 2 1 1 13.57 13.57 13.57 73.5 9 0 0 56300000.0 Hydrogen 2 (M) 11 DPEEALCWLSY (-) 11 D P\E-E\A\L\C\W\L/S/Y 81.8 9 6 2 0 1 b-ion bias Carbamidomethylation C:Carbamidomethylation, 0.987 100.0 99.5 46.23 0.0 691.8015 1382.593 0.0024 1.8 1.0 3.57 1513.7 nuORFdb_human ENST00000538962.7_1_9:114359649-114359685:- ENST00000538962.7_1_9:114359649-114359685:- PTGR1 Out-of-Frame Out-of-Frame GN=PTGR1 Out-of-Frame AL20221201_MKP_PDAC_01 Exploris_FAIMS VH20221207_MKP_FAIMS2022E_HLAc1_4e7_PDAC.64298.64298.2 2 1 1 16.98 2.55 11.21 14.44 87.1 9 0 0 15900000.0 Hydrogen 2 (M) 11 DPEEALCWLSY (-) 11 DKEnALMPnWY D P\E\E\A\L\C\W\L/S/Y 81.8 9 7 2 0 0 b-ion bias Carbamidomethylation C:Carbamidomethylation, 0.939 89.8 109.83 15.06 -50.0 691.7999 1382.593 -0.0008 -0.6 2.0 3.57 1513.7 nuORFdb_human ENST00000538962.7_1_9:114359649-114359685:- ENST00000538962.7_1_9:114359649-114359685:- PTGR1 Out-of-Frame Out-of-Frame GN=PTGR1 Out-of-Frame VH20221207_MKP_FAIMS2022E_HLAc1_4e7_PDAC SCP E_20221201_NO30_400nL_HLAc1_4e7_PDAC_Slot2-6_1_3524.25778.1338.2 2 6 1 15.5 3.11 11.31 12.39 82.5 8 1 1 56900.0 Hydrogen 5 (M) 9 DPWFNKYEF (C) 9 FSSGGQVKCEF D/P/W|F|N|K\Y\E\F 100.0 9 6 5 3 0 dual b/y bias 89.055275 0.914903 623.2807 1245.557 -0.0028 -2.2 0.19999999999999973 4.37 3624.0 nuORFdb_human ENST00000472152.5_1_1:79120733-79120823:+ ENST00000472152.5_1_1:79120733-79120823:+ IFI44 ncRNA Processed Transcript lncRNA GN=IFI44 ncRNA Processed Transcript E_20221201_NO30_400nL_HLAc1_4e7_PDAC_Slot2-6_1_3524 Exploris_FAIMS VH20221207_MKP_FAIMS2022E_HLAc1_4e7_PDAC.60398.60398.2 2 1 1 12.67 12.67 12.67 74.3 7 1 1 17600000.0 Hydrogen 5 (M) 9 DPWFNKYEF (C) 9 D P\W|F/N/K-Y-E/F 77.8 7 2 4 1 2 y-ion bias 0.974 92.2 104.62 120.0 -50.0 623.2805 1245.557 -0.0038 -3.0 -0.3999999999999999 4.37 3624.0 nuORFdb_human ENST00000472152.5_1_1:79120733-79120823:+ ENST00000472152.5_1_1:79120733-79120823:+ IFI44 ncRNA Processed Transcript lncRNA GN=IFI44 ncRNA Processed Transcript VH20221207_MKP_FAIMS2022E_HLAc1_4e7_PDAC SCP E_20221201_NO30_400nL_HLAc1_4e7_PDAC_Slot2-6_1_3524.28722.81.1 1 1 1 16.16 7.28 9.19 8.88 93.2 7 0 0 4140.0 Hydrogen 3 (E) 9 DYCLILHYL (-) 9 YPAAFDLWNL D Y/C|L|I|L|H\Y\L 77.8 7 6 5 4 0 dual b/y bias Carbamidomethylation C:Carbamidomethylation, 99.231415 1.672619 1209.5917 1209.597 -0.0073 -6.0 -3.6 5.08 1469.7 nuORFdb_human ENST00000302513.6_1_2:99767395-99767428:+ ENST00000302513.6_1_2:99767395-99767428:+ C2orf15 3' dORF 3' dORF GN=C2orf15 3' dORF E_20221201_NO30_400nL_HLAc1_4e7_PDAC_Slot2-6_1_3524 Eva-50mio-3fr AL20210827_EV_HLA_PDAC_PANFRO413_2D_50e6_W632_classI_fxn3.52813.52910.2 2 1 1 13.86 4.21 9.64 9.64 88.2 7 0 1 55400000.0 Hydrogen 19 (A) 9 DYFFKPVLF (-) 9 YnFKKTFNL D Y\F|F|K/P\V/L/F 77.8 7 4 5 2 0 unbiased 0.982 97.7 95.12 63.84 0.0 588.3115 1175.614 0.0022 1.8 1.3 5.83 3297.9 nuORFdb_human ENST00000369452.8_1_10:112723943-112724024:+ ENST00000369452.8_1_10:112723943-112724024:+ SHOC2 5' uORF 5' uORF GN=SHOC2 5' uORF AL20210827_EV_HLA_PDAC_PANFRO413_2D_50e6_W632_classI_fxn3 Exploris AL20221201_MKP_PDAC_01.66809.66809.2 2 1 1 14.84 3.9 10.5 10.94 88.5 7 0 1 311000000.0 Hydrogen 19 (A) 9 DYFFKPVLF (-) 9 YnFKKTFNL D Y\F|F|K/P\V/L/F 77.8 7 4 5 2 0 unbiased 0.986 92.3 100.05 3.06 0.0 588.3106 1175.614 0.0004 0.3 -0.5 5.83 3297.9 nuORFdb_human ENST00000369452.8_1_10:112723943-112724024:+ ENST00000369452.8_1_10:112723943-112724024:+ SHOC2 5' uORF 5' uORF GN=SHOC2 5' uORF AL20221201_MKP_PDAC_01 SCP E_20221201_NO30_400nL_HLAc1_4e7_PDAC_Slot2-6_1_3524.29104.88.1 1 1 1 9.21 -0.61 9.33 9.82 77.3 6 0 1 3260.0 Hydrogen 19 (A) 9 DYFFKPVLF (-) 9 FFKLLTmLm D Y/F\F|K|P V\L\F 55.6 5 5 3 2 0 unbiased 100.553452 1.66809 1175.6113 1175.614 -0.0036 -3.0 -0.6000000000000001 5.83 3297.9 nuORFdb_human ENST00000369452.8_1_10:112723943-112724024:+ ENST00000369452.8_1_10:112723943-112724024:+ SHOC2 5' uORF 5' uORF GN=SHOC2 5' uORF E_20221201_NO30_400nL_HLAc1_4e7_PDAC_Slot2-6_1_3524 Exploris AL20221201_MKP_PDAC_01.13390.13496.2 2 1 1 12.34 6.47 4.8 5.87 85.7 7 1 1 32500000.0 Hydrogen 15 (A) 9 EAAGEVRAM (-) 9 EAAGRDMLA E/A|A/G/E/V/R A/M 77.8 7 1 7 1 0 y-ion bias 0.977 100.0 24.15 100.0 0.0 467.2259 933.446 -0.0013 -1.4 -2.2 4.53 2447.7 smORF_v4|nuORFdb_human BTG1_12:92539535-92539603:- BTG1_12:92539535-92539603:-|ENST00000256015.4_1_12:92539535-92539610:- BTG1 uORF 12:92539535-92539603:- 12 BTG1 5' uORF 5' uORF GN=BTG1 AL20221201_MKP_PDAC_01 Exploris_FAIMS VH20221207_MKP_FAIMS2022E_HLAc1_4e7_PDAC.17685.17782.2 2 1 1 14.45 7.1 6.24 7.35 90.3 8 1 1 8540000.0 Hydrogen 15 (A) 9 EAAGEVRAM (-) 9 EAAGRDMLA E/A|A/G/E/V/R-A/M 100.0 9 1 7 1 1 y-ion bias 0.995 97.8 37.93 120.0 -70.0 467.2249 933.446 -0.0034 -3.6 -1.0 4.53 2447.7 smORF_v4|nuORFdb_human BTG1_12:92539535-92539603:- BTG1_12:92539535-92539603:-|ENST00000256015.4_1_12:92539535-92539610:- BTG1 uORF 12:92539535-92539603:- 12 BTG1 5' uORF 5' uORF GN=BTG1 VH20221207_MKP_FAIMS2022E_HLAc1_4e7_PDAC Exploris AL20221201_MKP_PDAC_01.7810.7810.2 2 1 1 8.65 2.86 6.49 5.79 65.7 6 1 1 6180000.0 M23m Hydrogen 15 (A) 9 EAAGEVRAm (-) 9 AEAGPVCVF E A|A/G/E/V/R A/m 55.6 5 1 6 1 0 y-ion bias m:Oxidized methionine 0.739 78.7 15.27 100.0 0.0 475.2258 933.446 15.9985 3.8 3.0 4.53 2447.7 smORF_v4|nuORFdb_human BTG1_12:92539535-92539603:- BTG1_12:92539535-92539603:-|ENST00000256015.4_1_12:92539535-92539610:- BTG1 uORF 12:92539535-92539603:- 12 BTG1 5' uORF 5' uORF GN=BTG1 AL20221201_MKP_PDAC_01 SCP E_20221201_NO30_400nL_HLAc1_4e7_PDAC_Slot2-6_1_3524.2816.1530.2 2 1 1 9.55 2.88 9.55 6.67 74.6 7 1 1 4050.0 M23m Hydrogen 15 (A) 9 EAAGEVRAm (-) 9 NDVEVRAm E/A/A|G/E/V/R\A m 77.8 7 2 6 1 0 y-ion bias m:Oxidized methionine 9.73702 0.794705 475.2214 933.446 15.9889 -6.4 -4.0 4.53 2447.7 smORF_v4|nuORFdb_human BTG1_12:92539535-92539603:- BTG1_12:92539535-92539603:-|ENST00000256015.4_1_12:92539535-92539610:- BTG1 uORF 12:92539535-92539603:- 12 BTG1 5' uORF 5' uORF GN=BTG1 E_20221201_NO30_400nL_HLAc1_4e7_PDAC_Slot2-6_1_3524 20mio-rep1 E_20221201_NO30_400nL_HLAc1_2e7_directIP_titration_rep1_Slot2-4_1_3515.22952.442.1 1 1 1 7.76 5.96 -0.27 1.79 56.5 6 0 0 4290.0 Hydrogen 29 (Q) 10 EAALLPLPSA (Q) 10 LPSPLLSPSA E A A L\L|P\L|P\S\A 60.0 6 6 2 2 0 b-ion bias 79.259789 1.460296 981.5568 981.562 -0.0045 -4.6 1.6000000000000005 4.0 4605.3 nuORFdb_human ENST00000311597.9_1_22:50523869-50523992:- ENST00000311597.9_1_22:50523869-50523992:- MLC1 5' uORF 5' uORF GN=MLC1 5' uORF E_20221201_NO30_400nL_HLAc1_2e7_directIP_titration_rep1_Slot2-4_1_3515 SCP E_20221201_NO30_400nL_HLAc1_4e7_PDAC_Slot2-6_1_3524.24005.342.1 1 1 1 11.27 8.71 5.14 2.56 77.4 6 0 0 11200.0 Hydrogen 248 (P) 9 EAIVLDVDY (K) 9 LDLAIDVnY E A I|V\L\D\V\D\Y 66.7 6 6 1 1 0 b-ion bias 82.930634 1.519834 1036.5173 1036.52 0.0003 0.3 2.6999999999999997 3.49 67856.0 nuORFdb_human ENST00000360806.9_2_22:21827645-21842641:- ENST00000360806.9_2_22:21827645-21842641:-|ENST00000450651.5_1_22:21830386-21841179:-|ENST00000463608.5_1_22:21830433-21838406:-|ENST00000462560.6_1_22:21830876-21842641:-|ENST00000467443.5_1_22:21831964-21846319:-|ENST00000486209.5_1_22:21832732-21838406:-|ENST00000618794.4_1_22:20390444-20398270:- PI4KAP2 Pseudogene Pseudogene GN=PI4KAP2 Pseudogene E_20221201_NO30_400nL_HLAc1_4e7_PDAC_Slot2-6_1_3524 Exploris AL20221201_MKP_PDAC_01.18334.18590.2 2 1 1 12.27 10.8 2.28 1.48 84.6 6 1 1 1330000000.0 Hydrogen 5 (W) 9 EAVARAEQL (R) 9 AVAREAGAEL E A|V A-R\A\E\Q|L 55.6 5 5 2 2 1 b-ion bias 0.993 79.0 32.38 11.06 0.0 493.7673 986.527 0.0009 0.9 0.09999999999999998 4.53 3096.3 nuORFdb_human ENST00000505073.5_1_4:91048743-91048815:+ ENST00000505073.5_1_4:91048743-91048815:+ CCSER1 5' uORF 5' uORF GN=CCSER1 5' uORF AL20221201_MKP_PDAC_01 SCP E_20221201_NO30_400nL_HLAc1_4e7_PDAC_Slot2-6_1_3524.19653.407.1 1 1 1 11.2 6.37 6.8 4.83 77.2 6 0 0 4680.0 Hydrogen 101 (L) 9 ECSGVILAY (C) 9 CEKnIALY E C/S G\V|I\L\A\Y 55.6 5 5 2 1 0 b-ion bias Carbamidomethylation C:Carbamidomethylation, 67.893509 1.481424 1011.4809 1011.482 -0.0016 -1.5 0.8999999999999999 4.0 14673.5 nuORFdb_human ENST00000578730.1_1_4:1245335-1281818:+ ENST00000578730.1_1_4:1245335-1281818:+|ENST00000526421.5_1_11:102188266-102190718:+ CTBP1-AS2 Antisense lncRNA GN=CTBP1-AS2 Antisense E_20221201_NO30_400nL_HLAc1_4e7_PDAC_Slot2-6_1_3524 10mio-rep1 E_20221201_NO30_400nL_HLAc1_1e7_directIP_titration_rep1_Slot2-3_1_3512.9362.1468.2 2 1 1 9.29 2.83 6.45 6.45 73.4 5 1 1 3260.0 Hydrogen 5 (E) 11 EEAAAAKGGVW (G) 11 EEAASPMLQI E E|A|A/A/A K G G V-W 36.4 4 2 4 2 1 y-ion bias 32.345047 0.83361 544.7668 1088.537 -0.0104 -9.5 -1.7999999999999998 4.53 2640.9 smORF_v4|nuORFdb_human CREBL2_12:12764856-12764932:+ CREBL2_12:12764856-12764932:+|ENST00000540224.1_1_12:12764783-12764930:+ CREBL2 uORF 12:12764856-12764932:+ 12 CREBL2 ncRNA Processed Transcript lncRNA GN=CREBL2 E_20221201_NO30_400nL_HLAc1_1e7_directIP_titration_rep1_Slot2-3_1_3512 10mio-rep2 E_20221201_NO30_400nL_HLAc1_1e7_directIP_titration_rep2_Slot2-3_1_3513.9318.1461.2 2 1 1 10.28 0.95 7.76 9.33 77.5 6 1 1 3170.0 Hydrogen 5 (E) 11 EEAAAAKGGVW (G) 11 qEAAIQAFLD E E/A|A/A|A K G G/V\W 45.5 5 3 5 2 0 unbiased 32.193012 0.838378 544.7679 1088.537 -0.0084 -7.7 -1.0 4.53 2640.9 smORF_v4|nuORFdb_human CREBL2_12:12764856-12764932:+ CREBL2_12:12764856-12764932:+|ENST00000540224.1_1_12:12764783-12764930:+ CREBL2 uORF 12:12764856-12764932:+ 12 CREBL2 ncRNA Processed Transcript lncRNA GN=CREBL2 E_20221201_NO30_400nL_HLAc1_1e7_directIP_titration_rep2_Slot2-3_1_3513 20mio-rep1 E_20221201_NO30_400nL_HLAc1_2e7_directIP_titration_rep1_Slot2-4_1_3515.9241.1459.2 2 1 1 14.06 9.45 8.02 4.6 87.1 6 1 1 6210.0 Hydrogen 5 (E) 11 EEAAAAKGGVW (G) 11 EAEAAKNALnG E E/A|A|A|A K G G\V\W 45.5 5 5 4 3 0 unbiased 31.921259 0.839242 544.7679 1088.537 -0.0084 -7.7 -1.5 4.53 2640.9 smORF_v4|nuORFdb_human CREBL2_12:12764856-12764932:+ CREBL2_12:12764856-12764932:+|ENST00000540224.1_1_12:12764783-12764930:+ CREBL2 uORF 12:12764856-12764932:+ 12 CREBL2 ncRNA Processed Transcript lncRNA GN=CREBL2 E_20221201_NO30_400nL_HLAc1_2e7_directIP_titration_rep1_Slot2-4_1_3515 20mio-rep3 E_20221201_NO30_400nL_HLAc1_2e7_directIP_titration_rep4_Slot2-4_1_3519.9274.1457.2 2 2 1 16.37 6.03 9.92 10.34 91.3 7 1 1 10500.0 Hydrogen 5 (E) 11 EEAAAAKGGVW (G) 11 EEAERGVLW E/E|A|A|A|A K/G G V\W 54.5 6 5 6 4 0 dual b/y bias 32.038521 0.840499 544.769 1088.537 -0.0064 -5.9 -0.10000000000000053 4.53 2640.9 smORF_v4|nuORFdb_human CREBL2_12:12764856-12764932:+ CREBL2_12:12764856-12764932:+|ENST00000540224.1_1_12:12764783-12764930:+ CREBL2 uORF 12:12764856-12764932:+ 12 CREBL2 ncRNA Processed Transcript lncRNA GN=CREBL2 E_20221201_NO30_400nL_HLAc1_2e7_directIP_titration_rep4_Slot2-4_1_3519 40mio-rep1 E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep1_Slot1-04_1_3496.9565.1431.2 2 1 1 15.45 5.61 9.27 9.84 90.8 6 1 1 14800.0 Hydrogen 5 (E) 11 EEAAAAKGGVW (G) 11 EEAASPMLQI E E/A|A|A|A K G G-V\W 45.5 5 4 4 3 1 unbiased 33.044144 0.856773 544.7711 1088.537 -0.0024 -2.2 1.0 4.53 2640.9 smORF_v4|nuORFdb_human CREBL2_12:12764856-12764932:+ CREBL2_12:12764856-12764932:+|ENST00000540224.1_1_12:12764783-12764930:+ CREBL2 uORF 12:12764856-12764932:+ 12 CREBL2 ncRNA Processed Transcript lncRNA GN=CREBL2 E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep1_Slot1-04_1_3496 40mio-rep2 E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep2_Slot2-5_1_3520.9240.1444.2 2 1 1 16.34 5.02 10.7 11.31 91.2 6 1 1 4350.0 Hydrogen 5 (E) 11 EEAAAAKGGVW (G) 11 EEAASPMLQI E E|A|A|A|A K/G G V\W 36.4 4 5 5 4 0 dual b/y bias 31.921816 0.848912 544.7703 1088.537 -0.0044 -4.0 0.9000000000000004 4.53 2640.9 smORF_v4|nuORFdb_human CREBL2_12:12764856-12764932:+ CREBL2_12:12764856-12764932:+|ENST00000540224.1_1_12:12764783-12764930:+ CREBL2 uORF 12:12764856-12764932:+ 12 CREBL2 ncRNA Processed Transcript lncRNA GN=CREBL2 E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep2_Slot2-5_1_3520 40mio-rep3 E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep3_Slot2-5_1_3521.9245.1447.2 2 1 1 17.02 5.35 11.67 11.67 92.5 7 1 1 9680.0 Hydrogen 5 (E) 11 EEAAAAKGGVW (G) 11 EEAASPMLQI E E|A|A|A|A K/G G/V\W 45.5 5 5 6 4 0 dual b/y bias 31.93997 0.846909 544.77 1088.537 -0.0044 -4.0 0.5999999999999996 4.53 2640.9 smORF_v4|nuORFdb_human CREBL2_12:12764856-12764932:+ CREBL2_12:12764856-12764932:+|ENST00000540224.1_1_12:12764783-12764930:+ CREBL2 uORF 12:12764856-12764932:+ 12 CREBL2 ncRNA Processed Transcript lncRNA GN=CREBL2 E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep3_Slot2-5_1_3521 40mio-rep4 E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep4_Slot2-5_1_3522.9255.1443.2 2 1 1 14.87 3.58 11.0 11.3 85.7 6 1 1 11000.0 Hydrogen 5 (E) 11 EEAAAAKGGVW (G) 11 EAEAAKNALnG E E/A|A|A|A K G G\V\W 45.5 5 5 4 3 0 unbiased 31.964802 0.849506 544.769 1088.537 -0.0064 -5.9 -1.2000000000000002 4.53 2640.9 smORF_v4|nuORFdb_human CREBL2_12:12764856-12764932:+ CREBL2_12:12764856-12764932:+|ENST00000540224.1_1_12:12764783-12764930:+ CREBL2 uORF 12:12764856-12764932:+ 12 CREBL2 ncRNA Processed Transcript lncRNA GN=CREBL2 E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep4_Slot2-5_1_3522 1mio-rep1 E_20221201_NO30_400nL_HLAc1_1e6_directIP_titration_rep1_Slot1-06_1_3498.17873.1484.2 2 3 1 14.17 8.09 10.49 6.08 78.5 7 1 1 6460.0 Hydrogen 42 (V) 9 EEAVVLRGL (-) 9 EAEVLVRGL E/E|A|V|V|L/R\G L 77.8 7 5 6 4 0 dual b/y bias 61.727776 0.823657 493.2848 985.568 -0.0049 -5.0 -0.09999999999999964 4.53 4975.7 nuORFdb_human ENST00000233615.6_2_2:74685568-74685718:+ ENST00000233615.6_2_2:74685568-74685718:+ WBP1 5' uORF 5' uORF GN=WBP1 5' uORF E_20221201_NO30_400nL_HLAc1_1e6_directIP_titration_rep1_Slot1-06_1_3498 10mio-rep1 E_20221201_NO30_400nL_HLAc1_1e7_directIP_titration_rep1_Slot2-3_1_3512.17613.1487.2 2 7 1 14.53 6.98 8.34 7.55 87.6 7 1 1 59100.0 Hydrogen 42 (V) 9 EEAVVLRGL (-) 9 EEAVILQAL E E/A|V|V|L/R-G\L 77.8 7 4 5 3 1 dual b/y bias 60.841312 0.821651 493.2836 985.568 -0.0069 -7.0 0.7000000000000002 4.53 4975.7 nuORFdb_human ENST00000233615.6_2_2:74685568-74685718:+ ENST00000233615.6_2_2:74685568-74685718:+ WBP1 5' uORF 5' uORF GN=WBP1 5' uORF E_20221201_NO30_400nL_HLAc1_1e7_directIP_titration_rep1_Slot2-3_1_3512 10mio-rep2 E_20221201_NO30_400nL_HLAc1_1e7_directIP_titration_rep2_Slot2-3_1_3513.17535.1487.2 2 6 1 13.93 8.4 8.21 5.53 84.4 6 1 1 52500.0 Hydrogen 42 (V) 9 EEAVVLRGL (-) 9 EAEVLVRGL E E/A|V|V|L/R\G L 55.6 5 4 5 3 0 dual b/y bias 60.570934 0.821983 493.2846 985.568 -0.0049 -5.0 1.7000000000000002 4.53 4975.7 nuORFdb_human ENST00000233615.6_2_2:74685568-74685718:+ ENST00000233615.6_2_2:74685568-74685718:+ WBP1 5' uORF 5' uORF GN=WBP1 5' uORF E_20221201_NO30_400nL_HLAc1_1e7_directIP_titration_rep2_Slot2-3_1_3513 10mio-rep3 E_20221201_NO30_400nL_HLAc1_1e7_directIP_titration_rep3_Slot2-3_1_3514.17503.1499.2 2 8 1 13.51 7.75 8.95 5.76 87.1 7 1 1 61800.0 Hydrogen 42 (V) 9 EEAVVLRGL (-) 9 EAEVLVRGL E/E|A|V|V/L/R G-L 77.8 7 3 6 3 1 dual b/y bias 60.460121 0.81397 493.2842 985.568 -0.0069 -7.0 -0.7000000000000002 4.53 4975.7 nuORFdb_human ENST00000233615.6_2_2:74685568-74685718:+ ENST00000233615.6_2_2:74685568-74685718:+ WBP1 5' uORF 5' uORF GN=WBP1 5' uORF E_20221201_NO30_400nL_HLAc1_1e7_directIP_titration_rep3_Slot2-3_1_3514 20mio-rep1 E_20221201_NO30_400nL_HLAc1_2e7_directIP_titration_rep1_Slot2-4_1_3515.17469.1483.2 2 9 1 15.08 8.16 7.72 6.92 88.3 6 1 1 77700.0 Hydrogen 42 (V) 9 EEAVVLRGL (-) 9 EEAVILQAL E E|A|V|V|L|R G-L 55.6 5 5 5 5 1 dual b/y bias 60.331631 0.824086 493.2863 985.568 -0.0029 -3.0 3.2 4.53 4975.7 nuORFdb_human ENST00000233615.6_2_2:74685568-74685718:+ ENST00000233615.6_2_2:74685568-74685718:+ WBP1 5' uORF 5' uORF GN=WBP1 5' uORF E_20221201_NO30_400nL_HLAc1_2e7_directIP_titration_rep1_Slot2-4_1_3515 20mio-rep2 E_20221201_NO30_400nL_HLAc1_2e7_directIP_titration_rep3_Slot2-4_1_3518.17539.1481.2 2 3 1 14.42 7.97 8.46 6.46 85.6 7 1 1 42800.0 Hydrogen 42 (V) 9 EEAVVLRGL (-) 9 EEAVILQAL E E|A|V|V|L|R-G-L 77.8 7 5 5 5 2 dual b/y bias 60.581806 0.82568 493.2851 985.568 -0.0049 -5.0 1.2999999999999998 4.53 4975.7 nuORFdb_human ENST00000233615.6_2_2:74685568-74685718:+ ENST00000233615.6_2_2:74685568-74685718:+ WBP1 5' uORF 5' uORF GN=WBP1 5' uORF E_20221201_NO30_400nL_HLAc1_2e7_directIP_titration_rep3_Slot2-4_1_3518 20mio-rep3 E_20221201_NO30_400nL_HLAc1_2e7_directIP_titration_rep4_Slot2-4_1_3519.17525.1489.2 2 6 1 16.61 8.75 12.08 7.86 90.0 8 1 1 67900.0 Hydrogen 42 (V) 9 EEAVVLRGL (-) 9 EAEVLVRGL E/E|A|V|V|L/R\G\L 100.0 9 6 6 4 0 dual b/y bias 60.530262 0.820731 493.2836 985.568 -0.0069 -7.0 -1.2000000000000002 4.53 4975.7 nuORFdb_human ENST00000233615.6_2_2:74685568-74685718:+ ENST00000233615.6_2_2:74685568-74685718:+ WBP1 5' uORF 5' uORF GN=WBP1 5' uORF E_20221201_NO30_400nL_HLAc1_2e7_directIP_titration_rep4_Slot2-4_1_3519 40mio-rep1 E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep1_Slot1-04_1_3496.17778.1456.2 2 5 1 17.77 9.05 11.92 8.72 90.4 6 1 1 149000.0 Hydrogen 42 (V) 9 EEAVVLRGL (-) 9 EAEVLVRGL E E|A|V|V|L|R\G L 55.6 5 6 5 5 0 dual b/y bias 61.412003 0.841206 493.286 985.568 -0.0029 -3.0 0.20000000000000018 4.53 4975.7 nuORFdb_human ENST00000233615.6_2_2:74685568-74685718:+ ENST00000233615.6_2_2:74685568-74685718:+ WBP1 5' uORF 5' uORF GN=WBP1 5' uORF E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep1_Slot1-04_1_3496 40mio-rep2 E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep2_Slot2-5_1_3520.17426.1476.2 2 3 1 14.3 8.23 11.12 6.07 81.5 7 1 1 134000.0 Hydrogen 42 (V) 9 EEAVVLRGL (-) 9 EAEVLVRGL E E|A|V|V|L/R\G\L 77.8 7 6 5 4 0 dual b/y bias 60.197491 0.828728 493.2853 985.568 -0.0049 -5.0 -0.09999999999999964 4.53 4975.7 nuORFdb_human ENST00000233615.6_2_2:74685568-74685718:+ ENST00000233615.6_2_2:74685568-74685718:+ WBP1 5' uORF 5' uORF GN=WBP1 5' uORF E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep2_Slot2-5_1_3520 40mio-rep3 E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep3_Slot2-5_1_3521.17459.1480.2 2 3 1 12.5 6.94 9.05 5.57 80.6 6 1 1 96900.0 Hydrogen 42 (V) 9 EEAVVLRGL (-) 9 EAEVLVRGL E/E|A|V|V|L/R G L 66.7 6 4 6 4 0 dual b/y bias 60.309948 0.826347 493.2845 985.568 -0.0049 -5.0 -0.40000000000000036 4.53 4975.7 nuORFdb_human ENST00000233615.6_2_2:74685568-74685718:+ ENST00000233615.6_2_2:74685568-74685718:+ WBP1 5' uORF 5' uORF GN=WBP1 5' uORF E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep3_Slot2-5_1_3521 40mio-rep4 E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep4_Slot2-5_1_3522.17437.1478.2 2 4 1 12.12 6.77 7.73 5.35 78.5 6 1 1 100000.0 Hydrogen 42 (V) 9 EEAVVLRGL (-) 9 EEAVILQAL E/E|A|V|V|L/R G L 66.7 6 4 6 4 0 dual b/y bias 60.218655 0.827386 493.2834 985.568 -0.0089 -9.1 -4.3999999999999995 4.53 4975.7 nuORFdb_human ENST00000233615.6_2_2:74685568-74685718:+ ENST00000233615.6_2_2:74685568-74685718:+ WBP1 5' uORF 5' uORF GN=WBP1 5' uORF E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep4_Slot2-5_1_3522 5mio-rep1 E_20221201_NO30_400nL_HLAc1_5e6_directIP_titration_rep1_Slot1-37_1_3502.17865.1476.2 2 6 1 12.4 6.26 8.09 6.14 83.0 6 1 1 29400.0 Hydrogen 42 (V) 9 EEAVVLRGL (-) 9 EAEVLVRGL E E/A|V|V|L/R-G L 55.6 5 3 5 3 1 dual b/y bias 61.707359 0.828326 493.2841 985.568 -0.0069 -7.0 0.0 4.53 4975.7 nuORFdb_human ENST00000233615.6_2_2:74685568-74685718:+ ENST00000233615.6_2_2:74685568-74685718:+ WBP1 5' uORF 5' uORF GN=WBP1 5' uORF E_20221201_NO30_400nL_HLAc1_5e6_directIP_titration_rep1_Slot1-37_1_3502 5mio-rep2 E_20221201_NO30_400nL_HLAc1_5e6_directIP_titration_rep2_Slot2-2_1_3510.17704.1492.2 2 1 1 11.3 8.58 5.52 2.72 81.6 6 1 1 5570.0 Hydrogen 42 (V) 9 EEAVVLRGL (-) 9 EEAVILQAL E/E|A|V|V/L/R G L 66.7 6 3 6 3 0 dual b/y bias 61.149826 0.81853 493.2825 985.568 -0.0109 -11.1 -2.4000000000000004 4.53 4975.7 nuORFdb_human ENST00000233615.6_2_2:74685568-74685718:+ ENST00000233615.6_2_2:74685568-74685718:+ WBP1 5' uORF 5' uORF GN=WBP1 5' uORF E_20221201_NO30_400nL_HLAc1_5e6_directIP_titration_rep2_Slot2-2_1_3510 5mio-rep3 E_20221201_NO30_400nL_HLAc1_5e6_directIP_titration_rep3_Slot2-2_1_3511.17717.1491.2 2 4 1 13.39 7.78 8.56 5.61 84.5 6 1 1 13700.0 Hydrogen 42 (V) 9 EEAVVLRGL (-) 9 EEGLVLQVV E E|A|V|V|L|R-G L 55.6 5 5 5 5 1 dual b/y bias 61.195518 0.818919 493.281 985.568 -0.0129 -13.1 -4.9 4.53 4975.7 nuORFdb_human ENST00000233615.6_2_2:74685568-74685718:+ ENST00000233615.6_2_2:74685568-74685718:+ WBP1 5' uORF 5' uORF GN=WBP1 5' uORF E_20221201_NO30_400nL_HLAc1_5e6_directIP_titration_rep3_Slot2-2_1_3511 20mio-rep1 E_20221201_NO30_400nL_HLAc1_2e7_directIP_titration_rep1_Slot2-4_1_3515.13436.1441.2 2 1 1 12.83 5.76 10.39 7.07 79.2 8 1 1 7600.0 Hydrogen 9 (R) 10 EEDGLKAASW (G) 10 EETVAAAAASW E E/D/G/L/K|A/A|S\W 80.0 8 3 7 2 0 y-ion bias 46.406834 0.850833 553.2586 1105.516 -0.0053 -4.8 1.4000000000000004 4.14 5794.5 smORF_v4|nuORFdb_human SLMAP_3:57743090-57743250:+ SLMAP_3:57743090-57743250:+|ENST00000295951.7_1_3:57742468-57743248:+ SLMAP uORF 3:57743090-57743250:+ 3 SLMAP 5' uORF 5' uORF GN=SLMAP E_20221201_NO30_400nL_HLAc1_2e7_directIP_titration_rep1_Slot2-4_1_3515 20mio-rep2 E_20221201_NO30_400nL_HLAc1_2e7_directIP_titration_rep3_Slot2-4_1_3518.13557.1436.2 2 1 1 13.07 6.61 12.01 6.46 82.9 7 1 1 5540.0 Hydrogen 9 (R) 10 EEDGLKAASW (G) 10 EETVAAAAASW E E|D/G/L/K|A A|S\W 60.0 6 4 6 3 0 unbiased 46.825665 0.853871 553.2582 1105.516 -0.0073 -6.6 -0.2999999999999998 4.14 5794.5 smORF_v4|nuORFdb_human SLMAP_3:57743090-57743250:+ SLMAP_3:57743090-57743250:+|ENST00000295951.7_1_3:57742468-57743248:+ SLMAP uORF 3:57743090-57743250:+ 3 SLMAP 5' uORF 5' uORF GN=SLMAP E_20221201_NO30_400nL_HLAc1_2e7_directIP_titration_rep3_Slot2-4_1_3518 20mio-rep3 E_20221201_NO30_400nL_HLAc1_2e7_directIP_titration_rep4_Slot2-4_1_3519.13459.1436.2 2 1 1 8.95 1.87 9.92 7.07 68.4 7 1 1 8100.0 Hydrogen 9 (R) 10 EEDGLKAASW (G) 10 AFSGARHSSW E E/D|G/L/K/A A|S\W 60.0 6 3 6 2 0 y-ion bias 46.490711 0.85355 553.2583 1105.516 -0.0073 -6.6 -0.7999999999999998 4.14 5794.5 smORF_v4|nuORFdb_human SLMAP_3:57743090-57743250:+ SLMAP_3:57743090-57743250:+|ENST00000295951.7_1_3:57742468-57743248:+ SLMAP uORF 3:57743090-57743250:+ 3 SLMAP 5' uORF 5' uORF GN=SLMAP E_20221201_NO30_400nL_HLAc1_2e7_directIP_titration_rep4_Slot2-4_1_3519 40mio-rep1 E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep1_Slot1-04_1_3496.13770.1417.2 2 1 1 9.56 2.41 9.56 7.15 73.4 8 1 1 8360.0 Hydrogen 9 (R) 10 EEDGLKAASW (G) 10 EETVAAAAASW E E/D/G/L/K/A-A|S\W 80.0 8 2 6 1 1 y-ion bias 47.565758 0.865404 553.2578 1105.516 -0.0073 -6.6 -3.3999999999999995 4.14 5794.5 smORF_v4|nuORFdb_human SLMAP_3:57743090-57743250:+ SLMAP_3:57743090-57743250:+|ENST00000295951.7_1_3:57742468-57743248:+ SLMAP uORF 3:57743090-57743250:+ 3 SLMAP 5' uORF 5' uORF GN=SLMAP E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep1_Slot1-04_1_3496 40mio-rep2 E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep2_Slot2-5_1_3520.13430.1428.2 2 1 1 10.58 3.2 9.71 7.37 77.3 7 1 1 9570.0 Hydrogen 9 (R) 10 EEDGLKAASW (G) 10 EETVAAAAASW E E/D/G/L/K\A A|S\W 60.0 6 3 5 1 0 unbiased 46.393181 0.858934 553.2585 1105.516 -0.0053 -4.8 0.10000000000000053 4.14 5794.5 smORF_v4|nuORFdb_human SLMAP_3:57743090-57743250:+ SLMAP_3:57743090-57743250:+|ENST00000295951.7_1_3:57742468-57743248:+ SLMAP uORF 3:57743090-57743250:+ 3 SLMAP 5' uORF 5' uORF GN=SLMAP E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep2_Slot2-5_1_3520 40mio-rep3 E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep3_Slot2-5_1_3521.13416.1428.2 2 1 1 13.17 6.67 11.37 6.5 82.3 8 1 1 8500.0 Hydrogen 9 (R) 10 EEDGLKAASW (G) 10 EETVAAAAASW E E|D/G/L/K|A/A|S\W 80.0 8 4 7 3 0 unbiased 46.349228 0.859085 553.2592 1105.516 -0.0053 -4.8 -0.20000000000000018 4.14 5794.5 smORF_v4|nuORFdb_human SLMAP_3:57743090-57743250:+ SLMAP_3:57743090-57743250:+|ENST00000295951.7_1_3:57742468-57743248:+ SLMAP uORF 3:57743090-57743250:+ 3 SLMAP 5' uORF 5' uORF GN=SLMAP E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep3_Slot2-5_1_3521 40mio-rep4 E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep4_Slot2-5_1_3522.13415.1425.2 2 1 1 12.93 7.15 12.3 5.78 82.8 7 1 1 8810.0 Hydrogen 9 (R) 10 EEDGLKAASW (G) 10 EETVAAAAASW E E|D/G L/K|A\A|S\W 60.0 6 5 5 3 0 unbiased 46.326748 0.86079 553.2586 1105.516 -0.0053 -4.8 -0.09999999999999964 4.14 5794.5 smORF_v4|nuORFdb_human SLMAP_3:57743090-57743250:+ SLMAP_3:57743090-57743250:+|ENST00000295951.7_1_3:57742468-57743248:+ SLMAP uORF 3:57743090-57743250:+ 3 SLMAP 5' uORF 5' uORF GN=SLMAP E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep4_Slot2-5_1_3522 40mio-rep1 E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep1_Slot1-04_1_3496.12283.1213.2 2 3 1 12.37 6.25 5.01 6.12 74.3 8 1 2 72900.0 Hydrogen 32 (Q) 11 EEFLGGKKTNW (W) 11 NGVFLTTGISNW E/E|F\L|G|G K-K/T N\W 63.6 7 5 5 3 1 unbiased 42.429802 0.993154 654.8316 1308.658 -0.0016 -1.2 2.0 6.24 5381.1 nuORFdb_human ENST00000396667.7_2_7:12254545-12258110:+ ENST00000396667.7_2_7:12254545-12258110:+ TMEM106B Out-of-Frame Out-of-Frame GN=TMEM106B Out-of-Frame E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep1_Slot1-04_1_3496 5mio-rep1 E_20221201_NO30_400nL_HLAc1_5e6_directIP_titration_rep1_Slot1-37_1_3502.12332.1356.2 2 1 1 7.44 7.44 7.44 54.0 7 1 2 1410.0 Hydrogen 32 (Q) 11 EEFLGGKKTNW (W) 11 E/E|F\L|G G K/K\T N\W 54.5 6 5 4 2 0 unbiased 42.599308 0.903596 654.8313 1308.658 -0.0036 -2.7 4.3 6.24 5381.1 nuORFdb_human ENST00000396667.7_2_7:12254545-12258110:+ ENST00000396667.7_2_7:12254545-12258110:+ TMEM106B Out-of-Frame Out-of-Frame GN=TMEM106B Out-of-Frame E_20221201_NO30_400nL_HLAc1_5e6_directIP_titration_rep1_Slot1-37_1_3502 10mio-rep2 E_20221201_NO30_400nL_HLAc1_1e7_directIP_titration_rep2_Slot2-3_1_3513.11045.1389.2 2 3 1 8.23 4.21 2.94 4.02 66.8 6 2 2 17400.0 Hydrogen 7 (K) 9 EEGRIITKW (-) 9 QNKVLDTKW E E/G R\I\I|T\K\W 55.6 5 5 2 1 0 b-ion bias 38.159161 0.883557 566.308 1131.616 -0.007 -6.2 0.5 6.24 1921.2 nuORFdb_human ENST00000230480.10_1_6:43742087-43745211:+ ENST00000230480.10_1_6:43742087-43745211:+ VEGFA Out-of-Frame Out-of-Frame GN=VEGFA Out-of-Frame E_20221201_NO30_400nL_HLAc1_1e7_directIP_titration_rep2_Slot2-3_1_3513 10mio-rep3 E_20221201_NO30_400nL_HLAc1_1e7_directIP_titration_rep3_Slot2-3_1_3514.11021.1599.3 3 3 1 11.32 5.73 6.09 5.59 84.7 7 2 2 14200.0 Hydrogen 7 (K) 9 EEGRIITKW (-) 9 HCRIIFSKA E/E/G/R I/I/T/K-W 77.8 7 0 6 0 1 y-ion bias 38.076832 0.751142 377.8748 1131.616 -0.0052 -4.6 1.7000000000000002 6.24 1921.2 nuORFdb_human ENST00000230480.10_1_6:43742087-43745211:+ ENST00000230480.10_1_6:43742087-43745211:+ VEGFA Out-of-Frame Out-of-Frame GN=VEGFA Out-of-Frame E_20221201_NO30_400nL_HLAc1_1e7_directIP_titration_rep3_Slot2-3_1_3514 20mio-rep1 E_20221201_NO30_400nL_HLAc1_2e7_directIP_titration_rep1_Slot2-4_1_3515.10939.1626.3 3 4 1 10.46 5.65 5.36 4.81 82.7 8 2 2 36300.0 Hydrogen 7 (K) 9 EEGRIITKW (-) 9 HCRIIFSKA E/E/G/R/I/I/T/K-W 100.0 9 0 7 0 1 y-ion bias 37.783165 0.734122 377.8745 1131.616 -0.0052 -4.6 1.6000000000000005 6.24 1921.2 nuORFdb_human ENST00000230480.10_1_6:43742087-43745211:+ ENST00000230480.10_1_6:43742087-43745211:+ VEGFA Out-of-Frame Out-of-Frame GN=VEGFA Out-of-Frame E_20221201_NO30_400nL_HLAc1_2e7_directIP_titration_rep1_Slot2-4_1_3515 20mio-rep2 E_20221201_NO30_400nL_HLAc1_2e7_directIP_titration_rep3_Slot2-4_1_3518.11081.1587.3 3 6 1 11.59 4.11 7.24 7.48 85.7 7 2 2 45300.0 Hydrogen 7 (K) 9 EEGRIITKW (-) 9 HCRIIFSKA E/E/G R/I/I|T/K-W 77.8 7 1 6 1 1 y-ion bias 38.27483 0.758375 377.8739 1131.616 -0.0082 -7.3 -1.0 6.24 1921.2 nuORFdb_human ENST00000230480.10_1_6:43742087-43745211:+ ENST00000230480.10_1_6:43742087-43745211:+ VEGFA Out-of-Frame Out-of-Frame GN=VEGFA Out-of-Frame E_20221201_NO30_400nL_HLAc1_2e7_directIP_titration_rep3_Slot2-4_1_3518 20mio-rep3 E_20221201_NO30_400nL_HLAc1_2e7_directIP_titration_rep4_Slot2-4_1_3519.10959.1588.3 3 4 1 9.56 3.91 6.25 5.65 85.5 8 2 2 29900.0 Hydrogen 7 (K) 9 EEGRIITKW (-) 9 HCRIIFSKA E/E/G/R/I/I/T/K-W 100.0 9 0 7 0 1 y-ion bias 37.859322 0.758169 377.8747 1131.616 -0.0052 -4.6 1.2000000000000002 6.24 1921.2 nuORFdb_human ENST00000230480.10_1_6:43742087-43745211:+ ENST00000230480.10_1_6:43742087-43745211:+ VEGFA Out-of-Frame Out-of-Frame GN=VEGFA Out-of-Frame E_20221201_NO30_400nL_HLAc1_2e7_directIP_titration_rep4_Slot2-4_1_3519 40mio-rep1 E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep1_Slot1-04_1_3496.11277.1357.2 2 4 1 15.6 7.42 7.48 8.18 88.9 7 2 2 150000.0 Hydrogen 7 (K) 9 EEGRIITKW (-) 9 QNKVLDTKW E/E/G R\I\I|T|K\W 77.8 7 5 4 2 0 unbiased 38.955662 0.903309 566.3106 1131.616 -0.001 -0.8 2.4000000000000004 6.24 1921.2 nuORFdb_human ENST00000230480.10_1_6:43742087-43745211:+ ENST00000230480.10_1_6:43742087-43745211:+ VEGFA Out-of-Frame Out-of-Frame GN=VEGFA Out-of-Frame E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep1_Slot1-04_1_3496 40mio-rep2 E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep2_Slot2-5_1_3520.10924.1592.3 3 3 1 13.47 4.36 7.87 9.11 89.9 8 2 2 54300.0 Hydrogen 7 (K) 9 EEGRIITKW (-) 9 HCRIIFSKA E/E/G/R/I/I/T/K\W 100.0 9 1 7 0 0 y-ion bias 37.738022 0.755237 377.8751 1131.616 -0.0052 -4.6 0.3000000000000007 6.24 1921.2 nuORFdb_human ENST00000230480.10_1_6:43742087-43745211:+ ENST00000230480.10_1_6:43742087-43745211:+ VEGFA Out-of-Frame Out-of-Frame GN=VEGFA Out-of-Frame E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep2_Slot2-5_1_3520 40mio-rep3 E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep3_Slot2-5_1_3521.10927.1585.3 3 3 1 13.88 7.48 6.99 6.4 88.7 8 2 2 25700.0 Hydrogen 7 (K) 9 EEGRIITKW (-) 9 HCRIIFSKA E/E/G/R/I/I/T/K-W 100.0 9 0 7 0 1 y-ion bias 37.751019 0.759739 377.8747 1131.616 -0.0052 -4.6 0.0 6.24 1921.2 nuORFdb_human ENST00000230480.10_1_6:43742087-43745211:+ ENST00000230480.10_1_6:43742087-43745211:+ VEGFA Out-of-Frame Out-of-Frame GN=VEGFA Out-of-Frame E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep3_Slot2-5_1_3521 40mio-rep3 E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep3_Slot2-5_1_3521.23605.280.1 1 1 1 9.01 4.25 5.85 4.76 72.2 6 0 0 3540.0 Hydrogen 44 (K) 9 EEIDDSIFY (-) 9 FEEVEDSLY E E I\D\D\S\I\F\Y 66.7 6 6 0 0 0 b-ion bias 81.541534 1.556047 1130.4849 1130.489 -0.0088 -7.8 -3.2 3.44 6384.0 nuORFdb_human ENST00000469754.2_1_13:50657287-50678952:+ ENST00000469754.2_1_13:50657287-50678952:+ DLEU1 3' Overlap dORF 3' Overlap dORF GN=DLEU1 3' Overlap dORF E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep3_Slot2-5_1_3521 20mio-rep1 E_20221201_NO30_400nL_HLAc1_2e7_directIP_titration_rep1_Slot2-4_1_3515.22507.1348.2 2 1 1 8.33 2.93 8.33 5.4 66.0 5 1 1 2600.0 Hydrogen 37 (M) 10 EEIGNIITKL (S) 10 EEINGTILKL E E/I/G N/I|I/T K L 30.0 3 1 5 1 0 y-ion bias 77.723305 0.908909 565.3218 1129.646 -0.0096 -8.5 -2.3 4.53 6292.9 nuORFdb_human ENST00000269205.5_1_18:29340660-29340837:- ENST00000269205.5_1_18:29340660-29340837:-|ENST00000377716.6_1_9:37888584-37899892:-|ENST00000496760.5_1_9:37881530-37899922:- SLC25A52 5' uORF 5' uORF GN=SLC25A52 5' uORF E_20221201_NO30_400nL_HLAc1_2e7_directIP_titration_rep1_Slot2-4_1_3515 40mio-rep1 E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep1_Slot1-04_1_3496.22780.1323.2 2 1 1 17.42 7.81 12.2 9.61 91.5 8 1 1 7580.0 Hydrogen 37 (M) 10 EEIGNIITKL (S) 10 EEINGTILKL E E|I|G|N/I|I/T/K-L 80.0 8 4 7 4 1 dual b/y bias 78.695023 0.924706 565.3234 1129.646 -0.0076 -6.7 -3.5 4.53 6292.9 nuORFdb_human ENST00000269205.5_1_18:29340660-29340837:- ENST00000269205.5_1_18:29340660-29340837:-|ENST00000377716.6_1_9:37888584-37899892:-|ENST00000496760.5_1_9:37881530-37899922:- SLC25A52 5' uORF 5' uORF GN=SLC25A52 5' uORF E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep1_Slot1-04_1_3496 40mio-rep2 E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep2_Slot2-5_1_3520.22561.1342.2 2 2 1 13.05 4.56 8.48 8.48 80.2 7 1 1 8790.0 Hydrogen 37 (M) 10 EEIGNIITKL (S) 10 GELQDILTKI E E/I/G/N/I|I|T K\L 60.0 6 3 6 2 0 y-ion bias 77.938736 0.912638 565.3243 1129.646 -0.0056 -5.0 -0.09999999999999964 4.53 6292.9 nuORFdb_human ENST00000269205.5_1_18:29340660-29340837:- ENST00000269205.5_1_18:29340660-29340837:-|ENST00000377716.6_1_9:37888584-37899892:-|ENST00000496760.5_1_9:37881530-37899922:- SLC25A52 5' uORF 5' uORF GN=SLC25A52 5' uORF E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep2_Slot2-5_1_3520 40mio-rep4 E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep4_Slot2-5_1_3522.22593.1342.2 2 1 1 16.77 9.41 10.82 7.36 91.1 8 1 1 3700.0 Hydrogen 37 (M) 10 EEIGNIITKL (S) 10 EEINGTILKL E E|I|G/N/I|I/T|K-L 80.0 8 4 7 4 1 dual b/y bias 78.031647 0.912448 565.3227 1129.646 -0.0076 -6.7 -2.0 4.53 6292.9 nuORFdb_human ENST00000269205.5_1_18:29340660-29340837:- ENST00000269205.5_1_18:29340660-29340837:-|ENST00000377716.6_1_9:37888584-37899892:-|ENST00000496760.5_1_9:37881530-37899922:- SLC25A52 5' uORF 5' uORF GN=SLC25A52 5' uORF E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep4_Slot2-5_1_3522 40mio-rep4 E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep4_Slot2-5_1_3522.9356.1595.3 3 1 1 7.8 2.07 5.58 5.73 73.1 7 2 2 7270.0 Hydrogen 27 (L) 9 EEIRITKAY (W) 9 EELKSLFGSI E/E/I R/I/T/K-A-Y 77.8 7 0 5 0 2 y-ion bias 32.3134 0.753661 374.8731 1122.615 -0.0109 -9.7 -4.999999999999999 6.24 10153.6 nuORFdb_human ENST00000505744.5_1_4:38792706-38805958:- ENST00000505744.5_1_4:38792706-38805958:-|ENST00000308979.6_1_4:38800499-38805961:-|ENST00000509754.5_1_4:38802433-38805961:- TLR1 ncRNA Processed Transcript lncRNA GN=TLR1 ncRNA Processed Transcript E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep4_Slot2-5_1_3522 40mio-rep1 E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep1_Slot1-04_1_3496.13639.1451.2 2 1 1 10.78 5.74 4.96 5.04 71.8 6 1 1 7240.0 Hydrogen 37 (K) 8 EEKNGFAW (L) 8 GFEEKNAW E/E/K/N G\F|A\W 75.0 6 3 4 1 0 unbiased 47.113449 0.844566 490.7269 980.447 -0.0005 -0.5 2.7 4.53 7678.9 nuORFdb_human ENST00000308086.2_1_12:72058272-72068101:+ ENST00000308086.2_1_12:72058272-72068101:+ THAP2 5' Overlap uORF 5' Overlap uORF GN=THAP2 5' Overlap uORF E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep1_Slot1-04_1_3496 40mio-rep1 E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep1_Slot1-04_1_3496.28744.236.1 1 1 1 8.1 0.02 7.64 8.08 70.2 6 0 0 5800.0 Hydrogen 16 (R) 9 EELLLNVEF (F) 9 ELELDAVIGF E E L\L|L\N\V\E-F 66.7 6 5 1 1 1 b-ion bias 99.301689 1.581835 1105.5724 1105.578 -0.0076 -6.8 -3.5999999999999996 3.67 3917.8 nuORFdb_human ENST00000367511.3_1_1:184859295-184863243:- ENST00000367511.3_1_1:184859295-184863243:- FAM129A Out-of-Frame Out-of-Frame GN=FAM129A Out-of-Frame E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep1_Slot1-04_1_3496 20mio-rep1 E_20221201_NO30_400nL_HLAc1_2e7_directIP_titration_rep1_Slot2-4_1_3515.12728.1360.2 2 2 1 12.16 4.06 10.9 8.1 78.7 6 1 1 13400.0 Hydrogen 87 (R) 9 EENCVKEWY (-) 9 CEEGGnKLWY E E|N/C/V\K E|W\Y 55.6 5 4 4 2 0 unbiased Carbamidomethylation C:Carbamidomethylation, 43.962502 0.901329 628.7624 1256.525 -0.0085 -6.8 -0.5999999999999996 4.25 10174.4 nuORFdb_human ENST00000264896.7_2_4:77116994-77134841:- ENST00000264896.7_2_4:77116994-77134841:- SCARB2 5' Overlap uORF 5' Overlap uORF GN=SCARB2 5' Overlap uORF E_20221201_NO30_400nL_HLAc1_2e7_directIP_titration_rep1_Slot2-4_1_3515 40mio-rep1 E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep1_Slot1-04_1_3496.13051.1323.2 2 2 1 12.56 3.8 10.83 8.76 78.2 7 1 1 15700.0 Hydrogen 87 (R) 9 EENCVKEWY (-) 9 CEEGGnKLWY E E/N/C|V\K/E|W\Y 77.8 7 4 5 2 0 unbiased Carbamidomethylation C:Carbamidomethylation, 45.082859 0.924551 628.7644 1256.525 -0.0045 -3.6 -0.3999999999999999 4.25 10174.4 nuORFdb_human ENST00000264896.7_2_4:77116994-77134841:- ENST00000264896.7_2_4:77116994-77134841:- SCARB2 5' Overlap uORF 5' Overlap uORF GN=SCARB2 5' Overlap uORF E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep1_Slot1-04_1_3496 40mio-rep2 E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep2_Slot2-5_1_3520.12704.1337.2 2 1 1 10.6 0.89 12.2 9.71 74.4 7 1 1 6380.0 Hydrogen 87 (R) 9 EENCVKEWY (-) 9 CEEGGnKLWY E E/N/C/V\K/E|W\Y 77.8 7 3 5 1 0 unbiased Carbamidomethylation C:Carbamidomethylation, 43.88628 0.915762 628.765 1256.525 -0.0025 -2.0 2.9000000000000004 4.25 10174.4 nuORFdb_human ENST00000264896.7_2_4:77116994-77134841:- ENST00000264896.7_2_4:77116994-77134841:- SCARB2 5' Overlap uORF 5' Overlap uORF GN=SCARB2 5' Overlap uORF E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep2_Slot2-5_1_3520 40mio-rep3 E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep3_Slot2-5_1_3521.12719.1333.2 2 2 1 11.72 2.53 12.82 9.19 76.3 6 1 1 16600.0 Hydrogen 87 (R) 9 EENCVKEWY (-) 9 CEEGGnKLWY E E|N/C|V K/E|W\Y 55.6 5 4 5 3 0 dual b/y bias Carbamidomethylation C:Carbamidomethylation, 43.941833 0.918461 628.7632 1256.525 -0.0065 -5.2 -0.6000000000000005 4.25 10174.4 nuORFdb_human ENST00000264896.7_2_4:77116994-77134841:- ENST00000264896.7_2_4:77116994-77134841:- SCARB2 5' Overlap uORF 5' Overlap uORF GN=SCARB2 5' Overlap uORF E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep3_Slot2-5_1_3521 40mio-rep4 E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep4_Slot2-5_1_3522.12715.1346.2 2 2 1 11.94 2.94 11.85 9.0 77.4 7 1 1 15000.0 Hydrogen 87 (R) 9 EENCVKEWY (-) 9 CEEGGnKLWY E E/N/C|V\K/E|W\Y 77.8 7 4 5 2 0 unbiased Carbamidomethylation C:Carbamidomethylation, 43.909092 0.909973 628.7632 1256.525 -0.0065 -5.2 -0.5 4.25 10174.4 nuORFdb_human ENST00000264896.7_2_4:77116994-77134841:- ENST00000264896.7_2_4:77116994-77134841:- SCARB2 5' Overlap uORF 5' Overlap uORF GN=SCARB2 5' Overlap uORF E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep4_Slot2-5_1_3522 40mio-rep1 E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep1_Slot1-04_1_3496.11757.1302.2 2 1 1 9.44 1.58 8.62 7.86 72.7 6 1 1 7330.0 C90c Hydrogen 87 (R) 9 EENcVKEWY (-) 9 cEEGGnKLWY E E/N/c/V|K E|W\Y 55.6 5 3 5 2 0 unbiased c:Cysteinylation 40.613277 0.9374 659.7552 1256.525 61.9775 -3.9 -0.6999999999999997 4.25 10174.4 nuORFdb_human ENST00000264896.7_2_4:77116994-77134841:- ENST00000264896.7_2_4:77116994-77134841:- SCARB2 5' Overlap uORF 5' Overlap uORF GN=SCARB2 5' Overlap uORF E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep1_Slot1-04_1_3496 40mio-rep2 E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep2_Slot2-5_1_3520.11423.1328.2 2 2 1 9.05 2.98 7.73 6.07 66.1 6 1 1 11600.0 C90c Hydrogen 87 (R) 9 EENcVKEWY (-) 9 cEEGGnKLWY E E/N\c/V/K E|W\Y 55.6 5 3 4 1 0 unbiased c:Cysteinylation 39.460392 0.921366 659.7545 1256.525 61.9775 -3.9 1.0000000000000004 4.25 10174.4 nuORFdb_human ENST00000264896.7_2_4:77116994-77134841:- ENST00000264896.7_2_4:77116994-77134841:- SCARB2 5' Overlap uORF 5' Overlap uORF GN=SCARB2 5' Overlap uORF E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep2_Slot2-5_1_3520 10mio-rep1 E_20221201_NO30_400nL_HLAc1_1e7_directIP_titration_rep1_Slot2-3_1_3512.18886.1436.2 2 1 1 12.22 7.79 2.59 4.43 88.3 5 0 0 5680.0 Hydrogen 41 (M) 9 EEPISLHTW (F) 9 EEPHLSLASE E E|P|I|S L/H\T W 33.3 3 4 4 3 0 dual b/y bias 65.238739 0.854006 556.2696 1111.542 -0.0091 -8.2 -0.4999999999999991 4.51 6961.0 nuORFdb_human ENST00000318410.11_1_8:126096196-126103965:- ENST00000318410.11_1_8:126096196-126103965:- WASHC5 5' uORF 5' uORF GN=WASHC5 5' uORF E_20221201_NO30_400nL_HLAc1_1e7_directIP_titration_rep1_Slot2-3_1_3512 10mio-rep2 E_20221201_NO30_400nL_HLAc1_1e7_directIP_titration_rep2_Slot2-3_1_3513.18849.1418.2 2 1 1 8.97 3.87 3.55 5.1 75.0 5 0 0 4710.0 Hydrogen 41 (M) 9 EEPISLHTW (F) 9 EERQQIPPD E E|P/I/S L/H/T W 33.3 3 1 5 1 0 y-ion bias 65.108711 0.865279 556.2715 1111.542 -0.0071 -6.4 0.2999999999999998 4.51 6961.0 nuORFdb_human ENST00000318410.11_1_8:126096196-126103965:- ENST00000318410.11_1_8:126096196-126103965:- WASHC5 5' uORF 5' uORF GN=WASHC5 5' uORF E_20221201_NO30_400nL_HLAc1_1e7_directIP_titration_rep2_Slot2-3_1_3513 10mio-rep3 E_20221201_NO30_400nL_HLAc1_1e7_directIP_titration_rep3_Slot2-3_1_3514.18766.1440.2 2 1 1 10.77 6.01 3.29 4.76 88.5 6 0 0 5170.0 Hydrogen 41 (M) 9 EEPISLHTW (F) 9 EEPRHLTTE E E|P/I/S L/H-T-W 55.6 5 1 4 1 2 y-ion bias 64.823936 0.851538 556.2716 1111.542 -0.0051 -4.6 1.7000000000000002 4.51 6961.0 nuORFdb_human ENST00000318410.11_1_8:126096196-126103965:- ENST00000318410.11_1_8:126096196-126103965:- WASHC5 5' uORF 5' uORF GN=WASHC5 5' uORF E_20221201_NO30_400nL_HLAc1_1e7_directIP_titration_rep3_Slot2-3_1_3514 20mio-rep1 E_20221201_NO30_400nL_HLAc1_2e7_directIP_titration_rep1_Slot2-4_1_3515.18771.1427.2 2 1 1 13.81 7.61 4.76 6.2 88.4 7 0 0 9900.0 Hydrogen 41 (M) 9 EEPISLHTW (F) 9 EHVLAVAnEE E/E|P/I|S L/H|T-W 77.8 7 3 6 3 1 dual b/y bias 64.826241 0.859323 556.2712 1111.542 -0.0071 -6.4 -0.20000000000000018 4.51 6961.0 nuORFdb_human ENST00000318410.11_1_8:126096196-126103965:- ENST00000318410.11_1_8:126096196-126103965:- WASHC5 5' uORF 5' uORF GN=WASHC5 5' uORF E_20221201_NO30_400nL_HLAc1_2e7_directIP_titration_rep1_Slot2-4_1_3515 20mio-rep2 E_20221201_NO30_400nL_HLAc1_2e7_directIP_titration_rep3_Slot2-4_1_3518.18837.1430.2 2 2 1 14.29 7.12 5.17 7.17 91.7 7 0 0 15900.0 Hydrogen 41 (M) 9 EEPISLHTW (F) 9 EEPGYVLFSA E E|P/I|S-L/H/T\W 77.8 7 3 5 2 1 unbiased 65.06398 0.857304 556.2708 1111.542 -0.0071 -6.4 -0.10000000000000053 4.51 6961.0 nuORFdb_human ENST00000318410.11_1_8:126096196-126103965:- ENST00000318410.11_1_8:126096196-126103965:- WASHC5 5' uORF 5' uORF GN=WASHC5 5' uORF E_20221201_NO30_400nL_HLAc1_2e7_directIP_titration_rep3_Slot2-4_1_3518 20mio-rep3 E_20221201_NO30_400nL_HLAc1_2e7_directIP_titration_rep4_Slot2-4_1_3519.18815.1427.2 2 1 1 13.19 6.9 5.24 6.28 89.4 7 0 0 8760.0 Hydrogen 41 (M) 9 EEPISLHTW (F) 9 EEPFHSVGLP E E|P/I/S-L|H/T-W 77.8 7 2 5 2 2 y-ion bias 64.983543 0.859696 556.2718 1111.542 -0.0051 -4.6 1.2000000000000002 4.51 6961.0 nuORFdb_human ENST00000318410.11_1_8:126096196-126103965:- ENST00000318410.11_1_8:126096196-126103965:- WASHC5 5' uORF 5' uORF GN=WASHC5 5' uORF E_20221201_NO30_400nL_HLAc1_2e7_directIP_titration_rep4_Slot2-4_1_3519 40mio-rep1 E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep1_Slot1-04_1_3496.19022.1399.2 2 2 1 11.72 6.26 4.69 5.46 88.6 7 0 0 26400.0 Hydrogen 41 (M) 9 EEPISLHTW (F) 9 EEPRHLTTE E E|P/I/S-L/H/T-W 77.8 7 1 5 1 2 y-ion bias 65.711273 0.876863 556.2751 1111.542 0.0009 0.8 4.0 4.51 6961.0 nuORFdb_human ENST00000318410.11_1_8:126096196-126103965:- ENST00000318410.11_1_8:126096196-126103965:- WASHC5 5' uORF 5' uORF GN=WASHC5 5' uORF E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep1_Slot1-04_1_3496 40mio-rep2 E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep2_Slot2-5_1_3520.18712.1421.2 2 5 1 14.24 6.9 5.2 7.34 90.7 7 0 0 53300.0 Hydrogen 41 (M) 9 EEPISLHTW (F) 9 EEPHLSLASE E E|P/I|S-L/H|T-W 77.8 7 3 5 3 2 dual b/y bias 64.6409 0.863287 556.2719 1111.542 -0.0051 -4.6 0.3000000000000007 4.51 6961.0 nuORFdb_human ENST00000318410.11_1_8:126096196-126103965:- ENST00000318410.11_1_8:126096196-126103965:- WASHC5 5' uORF 5' uORF GN=WASHC5 5' uORF E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep2_Slot2-5_1_3520 40mio-rep3 E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep3_Slot2-5_1_3521.18744.1427.2 2 4 1 15.0 8.67 3.87 6.33 92.4 6 0 0 30900.0 Hydrogen 41 (M) 9 EEPISLHTW (F) 9 EEPHLSLASE E E|P|I|S L/H/T-W 55.6 5 3 5 3 1 dual b/y bias 64.749733 0.859448 556.2717 1111.542 -0.0051 -4.6 0.0 4.51 6961.0 nuORFdb_human ENST00000318410.11_1_8:126096196-126103965:- ENST00000318410.11_1_8:126096196-126103965:- WASHC5 5' uORF 5' uORF GN=WASHC5 5' uORF E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep3_Slot2-5_1_3521 40mio-rep4 E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep4_Slot2-5_1_3522.18737.1427.2 2 4 1 13.8 7.1 4.71 6.7 92.7 7 0 0 37200.0 Hydrogen 41 (M) 9 EEPISLHTW (F) 9 EEPRHLTTE E E|P/I|S-L/H/T-W 77.8 7 2 5 2 2 y-ion bias 64.708626 0.859384 556.2716 1111.542 -0.0051 -4.6 0.10000000000000053 4.51 6961.0 nuORFdb_human ENST00000318410.11_1_8:126096196-126103965:- ENST00000318410.11_1_8:126096196-126103965:- WASHC5 5' uORF 5' uORF GN=WASHC5 5' uORF E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep4_Slot2-5_1_3522 5mio-rep1 E_20221201_NO30_400nL_HLAc1_5e6_directIP_titration_rep1_Slot1-37_1_3502.19133.1425.2 2 1 1 8.51 6.36 1.59 2.15 82.2 5 0 0 9470.0 Hydrogen 41 (M) 9 EEPISLHTW (F) 9 EERQQIPPD E E|P/I/S L/H T-W 33.3 3 1 4 1 1 y-ion bias 66.085701 0.860908 556.2715 1111.542 -0.0051 -4.6 2.4000000000000004 4.51 6961.0 nuORFdb_human ENST00000318410.11_1_8:126096196-126103965:- ENST00000318410.11_1_8:126096196-126103965:- WASHC5 5' uORF 5' uORF GN=WASHC5 5' uORF E_20221201_NO30_400nL_HLAc1_5e6_directIP_titration_rep1_Slot1-37_1_3502 40mio-rep1 E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep1_Slot1-04_1_3496.13500.1420.2 2 1 1 9.78 4.99 3.82 4.79 77.2 6 1 1 15200.0 Hydrogen 56 (A) 9 EEPLAGRTW (R) 9 PFRLGNPEE E E|P/L/A|G/R/T W 55.6 5 2 6 2 0 y-ion bias 46.63356 0.863936 529.7646 1058.526 -0.0038 -3.6 -0.3999999999999999 4.53 8015.0 nuORFdb_human ENST00000534515.1_1_11:67119307-67119526:- ENST00000534515.1_1_11:67119307-67119526:-|ENST00000529704.5_1_11:67119307-67120167:- POLD4 3' Overlap dORF 3' Overlap dORF GN=POLD4 3' Overlap dORF E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep1_Slot1-04_1_3496 40mio-rep3 E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep3_Slot2-5_1_3521.19564.1413.2 2 1 1 8.89 8.89 8.89 65.9 7 0 0 7880.0 C22c Hydrogen 15 (A) 9 EESAGFLcY (S) 9 E E/S/A/G/F/L|c-Y 77.8 7 1 6 1 1 y-ion bias c:Cysteinylation 67.584 0.868177 569.2122 1075.44 61.9766 -5.3 -0.7000000000000002 3.8 2739.9 nuORFdb_human ENST00000302313.9_1_3:39093509-39104563:+ ENST00000302313.9_1_3:39093509-39104563:+ WDR48 5' Overlap uORF 5' Overlap uORF GN=WDR48 5' Overlap uORF E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep3_Slot2-5_1_3521 20mio-rep3 E_20221201_NO30_400nL_HLAc1_2e7_directIP_titration_rep4_Slot2-4_1_3519.19427.592.1 1 1 1 9.7 4.15 4.84 5.55 70.7 6 0 0 1510.0 Hydrogen 22 (K) 8 EESPVVSW (R) 8 PDTVEVSW E E/S|P\V|V\S\W 75.0 6 5 3 2 0 unbiased 67.097107 1.371038 932.4302 932.436 -0.006 -6.4 -0.6000000000000005 3.8 6212.1 nuORFdb_human ENST00000474011.5_1_21:11060978-11098717:- ENST00000474011.5_1_21:11060978-11098717:-|ENST00000624155.2_1_GL000237.1:901-2487:-|ENST00000412295.2_1_5:180710378-180710423:+ BAGE2 Pseudogene Pseudogene GN=BAGE2 Pseudogene E_20221201_NO30_400nL_HLAc1_2e7_directIP_titration_rep4_Slot2-4_1_3519 40mio-rep1 E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep1_Slot1-04_1_3496.19686.564.1 1 2 1 12.16 6.0 4.02 6.16 84.9 5 0 0 10300.0 Hydrogen 22 (K) 8 EESPVVSW (R) 8 ETnVPVCL E E/S|P V|V|S\W 50.0 4 4 4 3 0 dual b/y bias 68.005463 1.387532 932.4333 932.436 -0.003 -3.2 0.0 3.8 6212.1 nuORFdb_human ENST00000474011.5_1_21:11060978-11098717:- ENST00000474011.5_1_21:11060978-11098717:-|ENST00000624155.2_1_GL000237.1:901-2487:-|ENST00000412295.2_1_5:180710378-180710423:+ BAGE2 Pseudogene Pseudogene GN=BAGE2 Pseudogene E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep1_Slot1-04_1_3496 40mio-rep2 E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep2_Slot2-5_1_3520.19369.581.1 1 1 1 11.38 6.61 2.64 4.77 84.5 5 0 0 5440.0 Hydrogen 22 (K) 8 EESPVVSW (R) 8 ETnVPVCL E E/S|P V|V\S\W 50.0 4 4 3 2 0 unbiased 66.910736 1.377668 932.4321 932.436 -0.004 -4.3 0.6000000000000005 3.8 6212.1 nuORFdb_human ENST00000474011.5_1_21:11060978-11098717:- ENST00000474011.5_1_21:11060978-11098717:-|ENST00000624155.2_1_GL000237.1:901-2487:-|ENST00000412295.2_1_5:180710378-180710423:+ BAGE2 Pseudogene Pseudogene GN=BAGE2 Pseudogene E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep2_Slot2-5_1_3520 40mio-rep3 E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep3_Slot2-5_1_3521.19394.578.1 1 1 1 10.99 5.22 5.77 5.77 86.9 5 0 0 3880.0 Hydrogen 22 (K) 8 EESPVVSW (R) 8 ETnVPVCL E E/S/P V|V\S\W 50.0 4 3 3 1 0 unbiased 66.994125 1.37956 932.4333 932.436 -0.003 -3.2 1.3999999999999995 3.8 6212.1 nuORFdb_human ENST00000474011.5_1_21:11060978-11098717:- ENST00000474011.5_1_21:11060978-11098717:-|ENST00000624155.2_1_GL000237.1:901-2487:-|ENST00000412295.2_1_5:180710378-180710423:+ BAGE2 Pseudogene Pseudogene GN=BAGE2 Pseudogene E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep3_Slot2-5_1_3521 40mio-rep4 E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep4_Slot2-5_1_3522.19372.582.1 1 2 1 11.23 5.29 6.44 5.94 80.7 6 0 0 7340.0 Hydrogen 22 (K) 8 EESPVVSW (R) 8 VSPDLESW E E/S/P\V\V|S\W 75.0 6 4 3 1 0 unbiased 66.90197 1.376994 932.4285 932.436 -0.008 -8.6 -3.8999999999999995 3.8 6212.1 nuORFdb_human ENST00000474011.5_1_21:11060978-11098717:- ENST00000474011.5_1_21:11060978-11098717:-|ENST00000624155.2_1_GL000237.1:901-2487:-|ENST00000412295.2_1_5:180710378-180710423:+ BAGE2 Pseudogene Pseudogene GN=BAGE2 Pseudogene E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep4_Slot2-5_1_3522 10mio-rep1 E_20221201_NO30_400nL_HLAc1_1e7_directIP_titration_rep1_Slot2-3_1_3512.14394.1387.2 2 1 1 9.35 4.75 6.65 4.6 66.1 6 1 1 5330.0 Hydrogen 12 (H) 9 EETEVWKDY (Y) 9 ENGNVPVEnPE E E/T|E-V/W|K\D-Y 77.8 7 3 4 2 2 unbiased 49.725113 0.884522 599.7613 1198.526 -0.0115 -9.6 -1.8999999999999995 4.0 4447.8 nuORFdb_human ENST00000504811.5_1_5:118813142-118814588:+ ENST00000504811.5_1_5:118813142-118814588:+ HSD17B4 Out-of-Frame Out-of-Frame GN=HSD17B4 Out-of-Frame E_20221201_NO30_400nL_HLAc1_1e7_directIP_titration_rep1_Slot2-3_1_3512 10mio-rep2 E_20221201_NO30_400nL_HLAc1_1e7_directIP_titration_rep2_Slot2-3_1_3513.14365.1391.2 2 2 1 14.32 6.68 11.19 7.64 84.2 7 1 1 7920.0 Hydrogen 12 (H) 9 EETEVWKDY (Y) 9 PPFDMWKDY E E|T/E|V|W|K/D\Y 77.8 7 5 6 4 0 dual b/y bias 49.624752 0.882032 599.7622 1198.526 -0.0095 -8.0 -1.2999999999999998 4.0 4447.8 nuORFdb_human ENST00000504811.5_1_5:118813142-118814588:+ ENST00000504811.5_1_5:118813142-118814588:+ HSD17B4 Out-of-Frame Out-of-Frame GN=HSD17B4 Out-of-Frame E_20221201_NO30_400nL_HLAc1_1e7_directIP_titration_rep2_Slot2-3_1_3513 10mio-rep3 E_20221201_NO30_400nL_HLAc1_1e7_directIP_titration_rep3_Slot2-3_1_3514.14310.1396.2 2 1 1 13.62 5.46 10.81 8.17 75.9 7 1 1 4200.0 Hydrogen 12 (H) 9 EETEVWKDY (Y) 9 PPFDMWKDY E E|T/E|V|W|K/D\Y 77.8 7 5 6 4 0 dual b/y bias 49.436947 0.87868 599.7634 1198.526 -0.0075 -6.3 0.0 4.0 4447.8 nuORFdb_human ENST00000504811.5_1_5:118813142-118814588:+ ENST00000504811.5_1_5:118813142-118814588:+ HSD17B4 Out-of-Frame Out-of-Frame GN=HSD17B4 Out-of-Frame E_20221201_NO30_400nL_HLAc1_1e7_directIP_titration_rep3_Slot2-3_1_3514 20mio-rep1 E_20221201_NO30_400nL_HLAc1_2e7_directIP_titration_rep1_Slot2-4_1_3515.14294.1382.2 2 1 1 12.52 4.93 8.06 7.59 79.2 6 1 1 8420.0 Hydrogen 12 (H) 9 EETEVWKDY (Y) 9 ENGNVPVEnPE E E|T/E|V|W|K D\Y 55.6 5 5 5 4 0 dual b/y bias 49.369133 0.887493 599.762 1198.526 -0.0095 -8.0 -1.7999999999999998 4.0 4447.8 nuORFdb_human ENST00000504811.5_1_5:118813142-118814588:+ ENST00000504811.5_1_5:118813142-118814588:+ HSD17B4 Out-of-Frame Out-of-Frame GN=HSD17B4 Out-of-Frame E_20221201_NO30_400nL_HLAc1_2e7_directIP_titration_rep1_Slot2-4_1_3515 20mio-rep2 E_20221201_NO30_400nL_HLAc1_2e7_directIP_titration_rep3_Slot2-4_1_3518.14393.1382.2 2 1 1 14.75 5.96 10.01 8.79 85.5 7 1 1 7430.0 Hydrogen 12 (H) 9 EETEVWKDY (Y) 9 PPFDMWKDY E E/T/E|V|W|K/D\Y 77.8 7 4 6 3 0 dual b/y bias 49.715523 0.887635 599.7632 1198.526 -0.0075 -6.3 0.0 4.0 4447.8 nuORFdb_human ENST00000504811.5_1_5:118813142-118814588:+ ENST00000504811.5_1_5:118813142-118814588:+ HSD17B4 Out-of-Frame Out-of-Frame GN=HSD17B4 Out-of-Frame E_20221201_NO30_400nL_HLAc1_2e7_directIP_titration_rep3_Slot2-4_1_3518 20mio-rep3 E_20221201_NO30_400nL_HLAc1_2e7_directIP_titration_rep4_Slot2-4_1_3519.14305.1380.2 2 1 1 13.59 5.08 10.71 8.51 82.5 7 1 1 7740.0 Hydrogen 12 (H) 9 EETEVWKDY (Y) 9 PPFDMWKDY E E/T|E|V|W|K/D\Y 77.8 7 5 6 4 0 dual b/y bias 49.410664 0.888866 599.7644 1198.526 -0.0055 -4.6 1.2000000000000002 4.0 4447.8 nuORFdb_human ENST00000504811.5_1_5:118813142-118814588:+ ENST00000504811.5_1_5:118813142-118814588:+ HSD17B4 Out-of-Frame Out-of-Frame GN=HSD17B4 Out-of-Frame E_20221201_NO30_400nL_HLAc1_2e7_directIP_titration_rep4_Slot2-4_1_3519 40mio-rep1 E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep1_Slot1-04_1_3496.14611.1341.2 2 2 1 17.04 6.4 12.12 10.64 83.2 7 1 1 19200.0 Hydrogen 12 (H) 9 EETEVWKDY (Y) 9 EEESLKDWY E E|T/E|V|W/K/D\Y 77.8 7 4 6 3 0 dual b/y bias 50.470753 0.912992 599.7649 1198.526 -0.0035 -3.0 0.20000000000000018 4.0 4447.8 nuORFdb_human ENST00000504811.5_1_5:118813142-118814588:+ ENST00000504811.5_1_5:118813142-118814588:+ HSD17B4 Out-of-Frame Out-of-Frame GN=HSD17B4 Out-of-Frame E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep1_Slot1-04_1_3496 40mio-rep2 E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep2_Slot2-5_1_3520.14258.1359.2 2 1 1 15.95 6.8 13.86 9.15 80.8 7 1 1 13700.0 Hydrogen 12 (H) 9 EETEVWKDY (Y) 9 PPFDMWKDY E E|T/E|V|W|K|D\Y 77.8 7 6 6 5 0 dual b/y bias 49.253086 0.901767 599.7643 1198.526 -0.0055 -4.6 0.3000000000000007 4.0 4447.8 nuORFdb_human ENST00000504811.5_1_5:118813142-118814588:+ ENST00000504811.5_1_5:118813142-118814588:+ HSD17B4 Out-of-Frame Out-of-Frame GN=HSD17B4 Out-of-Frame E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep2_Slot2-5_1_3520 40mio-rep3 E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep3_Slot2-5_1_3521.14270.1366.2 2 1 1 10.72 5.07 6.61 5.65 75.7 6 1 1 14700.0 Hydrogen 12 (H) 9 EETEVWKDY (Y) 9 ENGNVPVEnPE E E/T E|V|W/K/D\Y 55.6 5 3 5 2 0 unbiased 49.297752 0.897925 599.7645 1198.526 -0.0055 -4.6 0.0 4.0 4447.8 nuORFdb_human ENST00000504811.5_1_5:118813142-118814588:+ ENST00000504811.5_1_5:118813142-118814588:+ HSD17B4 Out-of-Frame Out-of-Frame GN=HSD17B4 Out-of-Frame E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep3_Slot2-5_1_3521 40mio-rep4 E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep4_Slot2-5_1_3522.14252.1366.2 2 2 1 15.23 6.63 11.29 8.6 84.2 7 1 1 21800.0 Hydrogen 12 (H) 9 EETEVWKDY (Y) 9 PPFDMWKDY E E/T/E|V|W|K|D\Y 77.8 7 5 6 4 0 dual b/y bias 49.221539 0.897822 599.765 1198.526 -0.0035 -3.0 1.7000000000000002 4.0 4447.8 nuORFdb_human ENST00000504811.5_1_5:118813142-118814588:+ ENST00000504811.5_1_5:118813142-118814588:+ HSD17B4 Out-of-Frame Out-of-Frame GN=HSD17B4 Out-of-Frame E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep4_Slot2-5_1_3522 10mio-rep1 E_20221201_NO30_400nL_HLAc1_1e7_directIP_titration_rep1_Slot2-3_1_3512.16421.1378.2 2 1 1 12.33 0.25 10.4 12.08 72.8 7 1 1 2790.0 Hydrogen 13 (M) 9 EEVCEKLTW (-) 9 LSDSQEDITW E E|V|C/E/K/L/T\W 77.8 7 3 6 2 0 y-ion bias Carbamidomethylation C:Carbamidomethylation, 56.725773 0.890365 597.2766 1193.551 -0.004 -3.3 4.4 4.25 2485.8 nuORFdb_human ENST00000367808.7_1_1:169337452-169337518:+ ENST00000367808.7_1_1:169337452-169337518:+ BLZF1 5' uORF 5' uORF GN=BLZF1 5' uORF E_20221201_NO30_400nL_HLAc1_1e7_directIP_titration_rep1_Slot2-3_1_3512 10mio-rep2 E_20221201_NO30_400nL_HLAc1_1e7_directIP_titration_rep2_Slot2-3_1_3513.16385.1381.2 2 2 1 9.71 2.29 7.01 7.42 71.8 6 1 1 5960.0 Hydrogen 13 (M) 9 EEVCEKLTW (-) 9 GNSQGKTSQTW E E|V/C|E/K L|T\W 55.6 5 4 5 3 0 dual b/y bias Carbamidomethylation C:Carbamidomethylation, 56.600716 0.888029 597.2764 1193.551 -0.006 -5.0 1.7000000000000002 4.25 2485.8 nuORFdb_human ENST00000367808.7_1_1:169337452-169337518:+ ENST00000367808.7_1_1:169337452-169337518:+ BLZF1 5' uORF 5' uORF GN=BLZF1 5' uORF E_20221201_NO30_400nL_HLAc1_1e7_directIP_titration_rep2_Slot2-3_1_3513 10mio-rep3 E_20221201_NO30_400nL_HLAc1_1e7_directIP_titration_rep3_Slot2-3_1_3514.16324.1383.2 2 1 1 10.36 9.67 10.36 73.6 7 1 1 3740.0 Hydrogen 13 (M) 9 EEVCEKLTW (-) 9 E E/V|C/E/K-L|T\W 77.8 7 3 5 2 1 unbiased Carbamidomethylation C:Carbamidomethylation, 56.390301 0.88705 597.2742 1193.551 -0.01 -8.4 -2.1000000000000005 4.25 2485.8 nuORFdb_human ENST00000367808.7_1_1:169337452-169337518:+ ENST00000367808.7_1_1:169337452-169337518:+ BLZF1 5' uORF 5' uORF GN=BLZF1 5' uORF E_20221201_NO30_400nL_HLAc1_1e7_directIP_titration_rep3_Slot2-3_1_3514 20mio-rep1 E_20221201_NO30_400nL_HLAc1_2e7_directIP_titration_rep1_Slot2-4_1_3515.16317.1363.2 2 3 1 15.03 3.05 10.37 11.98 87.7 6 1 1 16600.0 Hydrogen 13 (M) 9 EEVCEKLTW (-) 9 IEnCKEEIAS E E|V|C|E/K L|T\W 55.6 5 5 5 4 0 dual b/y bias Carbamidomethylation C:Carbamidomethylation, 56.352787 0.899483 597.2766 1193.551 -0.004 -3.3 2.9000000000000004 4.25 2485.8 nuORFdb_human ENST00000367808.7_1_1:169337452-169337518:+ ENST00000367808.7_1_1:169337452-169337518:+ BLZF1 5' uORF 5' uORF GN=BLZF1 5' uORF E_20221201_NO30_400nL_HLAc1_2e7_directIP_titration_rep1_Slot2-4_1_3515 20mio-rep2 E_20221201_NO30_400nL_HLAc1_2e7_directIP_titration_rep3_Slot2-4_1_3518.16415.1359.2 2 3 1 13.56 1.17 11.88 12.39 80.2 6 1 1 21900.0 Hydrogen 13 (M) 9 EEVCEKLTW (-) 9 GNSQGKTSQTW E E|V|C|E/K L|T\W 55.6 5 5 5 4 0 dual b/y bias Carbamidomethylation C:Carbamidomethylation, 56.696613 0.902213 597.2758 1193.551 -0.006 -5.0 1.2999999999999998 4.25 2485.8 nuORFdb_human ENST00000367808.7_1_1:169337452-169337518:+ ENST00000367808.7_1_1:169337452-169337518:+ BLZF1 5' uORF 5' uORF GN=BLZF1 5' uORF E_20221201_NO30_400nL_HLAc1_2e7_directIP_titration_rep3_Slot2-4_1_3518 20mio-rep3 E_20221201_NO30_400nL_HLAc1_2e7_directIP_titration_rep4_Slot2-4_1_3519.16363.1364.2 2 1 1 14.73 3.73 10.27 11.0 84.6 6 1 1 7180.0 Hydrogen 13 (M) 9 EEVCEKLTW (-) 9 GNSQGKTSQTW E E|V/C|E/K L|T\W 55.6 5 4 5 3 0 dual b/y bias Carbamidomethylation C:Carbamidomethylation, 56.518112 0.898637 597.2765 1193.551 -0.004 -3.3 2.5 4.25 2485.8 nuORFdb_human ENST00000367808.7_1_1:169337452-169337518:+ ENST00000367808.7_1_1:169337452-169337518:+ BLZF1 5' uORF 5' uORF GN=BLZF1 5' uORF E_20221201_NO30_400nL_HLAc1_2e7_directIP_titration_rep4_Slot2-4_1_3519 40mio-rep1 E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep1_Slot1-04_1_3496.16603.1325.2 2 1 1 15.22 11.21 15.22 84.7 7 1 1 15500.0 Hydrogen 13 (M) 9 EEVCEKLTW (-) 9 E E|V|C|E/K\L|T\W 77.8 7 6 5 4 0 dual b/y bias Carbamidomethylation C:Carbamidomethylation, 57.351711 0.92302 597.2781 1193.551 -0.002 -1.7 1.5000000000000002 4.25 2485.8 nuORFdb_human ENST00000367808.7_1_1:169337452-169337518:+ ENST00000367808.7_1_1:169337452-169337518:+ BLZF1 5' uORF 5' uORF GN=BLZF1 5' uORF E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep1_Slot1-04_1_3496 40mio-rep2 E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep2_Slot2-5_1_3520.16272.1355.2 2 3 1 15.04 11.61 15.04 81.3 6 1 1 38100.0 Hydrogen 13 (M) 9 EEVCEKLTW (-) 9 E E|V|C|E/K L|T\W 55.6 5 5 5 4 0 dual b/y bias Carbamidomethylation C:Carbamidomethylation, 56.208939 0.904391 597.2777 1193.551 -0.002 -1.7 3.2 4.25 2485.8 nuORFdb_human ENST00000367808.7_1_1:169337452-169337518:+ ENST00000367808.7_1_1:169337452-169337518:+ BLZF1 5' uORF 5' uORF GN=BLZF1 5' uORF E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep2_Slot2-5_1_3520 40mio-rep3 E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep3_Slot2-5_1_3521.16316.1341.2 2 2 1 10.88 10.88 10.88 70.3 6 1 1 20000.0 Hydrogen 13 (M) 9 EEVCEKLTW (-) 9 E E/V|C|E/K L|T\W 55.6 5 4 5 3 0 dual b/y bias Carbamidomethylation C:Carbamidomethylation, 56.362576 0.913531 597.2765 1193.551 -0.004 -3.3 1.2999999999999998 4.25 2485.8 nuORFdb_human ENST00000367808.7_1_1:169337452-169337518:+ ENST00000367808.7_1_1:169337452-169337518:+ BLZF1 5' uORF 5' uORF GN=BLZF1 5' uORF E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep3_Slot2-5_1_3521 40mio-rep4 E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep4_Slot2-5_1_3522.16272.1340.2 2 2 1 13.46 -0.39 10.81 13.85 81.0 7 1 1 16700.0 Hydrogen 13 (M) 9 EEVCEKLTW (-) 9 GNSQGKTSQTW E E|V|C/E/K-L|T\W 77.8 7 4 5 3 1 dual b/y bias Carbamidomethylation C:Carbamidomethylation, 56.195068 0.914074 597.2787 1193.551 0.0 0.0 4.7 4.25 2485.8 nuORFdb_human ENST00000367808.7_1_1:169337452-169337518:+ ENST00000367808.7_1_1:169337452-169337518:+ BLZF1 5' uORF 5' uORF GN=BLZF1 5' uORF E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep4_Slot2-5_1_3522 10mio-rep1 E_20221201_NO30_400nL_HLAc1_1e7_directIP_titration_rep1_Slot2-3_1_3512.15246.1348.2 2 2 1 7.79 4.76 7.79 57.9 6 1 1 7610.0 C16c Hydrogen 13 (M) 9 EEVcEKLTW (-) 9 E E|V|c/E/K L|T\W 55.6 5 4 5 3 0 dual b/y bias c:Cysteinylation 52.668598 0.908826 628.2654 1193.551 61.972 -8.5 -0.7999999999999998 4.25 2485.8 nuORFdb_human ENST00000367808.7_1_1:169337452-169337518:+ ENST00000367808.7_1_1:169337452-169337518:+ BLZF1 5' uORF 5' uORF GN=BLZF1 5' uORF E_20221201_NO30_400nL_HLAc1_1e7_directIP_titration_rep1_Slot2-3_1_3512 10mio-rep3 E_20221201_NO30_400nL_HLAc1_1e7_directIP_titration_rep3_Slot2-3_1_3514.15137.1346.2 2 1 1 8.01 8.01 8.01 58.0 6 1 1 3840.0 C16c Hydrogen 13 (M) 9 EEVcEKLTW (-) 9 E E|V|c/E/K L|T\W 55.6 5 4 5 3 0 dual b/y bias c:Cysteinylation 52.292473 0.910338 628.2642 1193.551 61.97 -10.0 -3.7 4.25 2485.8 nuORFdb_human ENST00000367808.7_1_1:169337452-169337518:+ ENST00000367808.7_1_1:169337452-169337518:+ BLZF1 5' uORF 5' uORF GN=BLZF1 5' uORF E_20221201_NO30_400nL_HLAc1_1e7_directIP_titration_rep3_Slot2-3_1_3514 20mio-rep2 E_20221201_NO30_400nL_HLAc1_2e7_directIP_titration_rep3_Slot2-4_1_3518.15201.1337.2 2 1 1 7.44 5.12 7.44 56.6 6 1 1 4470.0 C16c Hydrogen 13 (M) 9 EEVcEKLTW (-) 9 E E/V/c E\K-L|T\W 55.6 5 3 3 1 1 unbiased c:Cysteinylation 52.505112 0.916011 628.268 1193.551 61.978 -3.7 2.5999999999999996 4.25 2485.8 nuORFdb_human ENST00000367808.7_1_1:169337452-169337518:+ ENST00000367808.7_1_1:169337452-169337518:+ BLZF1 5' uORF 5' uORF GN=BLZF1 5' uORF E_20221201_NO30_400nL_HLAc1_2e7_directIP_titration_rep3_Slot2-4_1_3518 20mio-rep3 E_20221201_NO30_400nL_HLAc1_2e7_directIP_titration_rep4_Slot2-4_1_3519.15142.1334.2 2 1 1 10.22 -2.99 10.87 13.21 73.3 7 1 1 6950.0 C16c Hydrogen 13 (M) 9 EEVcEKLTW (-) 9 GHVFNmPHDDA E E/V|c/E/K-L|T\W 77.8 7 3 5 2 1 unbiased c:Cysteinylation 52.303074 0.91735 628.267 1193.551 61.976 -5.3 0.5 4.25 2485.8 nuORFdb_human ENST00000367808.7_1_1:169337452-169337518:+ ENST00000367808.7_1_1:169337452-169337518:+ BLZF1 5' uORF 5' uORF GN=BLZF1 5' uORF E_20221201_NO30_400nL_HLAc1_2e7_directIP_titration_rep4_Slot2-4_1_3519 40mio-rep1 E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep1_Slot1-04_1_3496.15411.1304.2 2 2 1 16.92 3.37 13.34 13.55 89.0 7 1 1 47300.0 C16c Hydrogen 13 (M) 9 EEVcEKLTW (-) 9 EEVTDDSSFLGG E/E|V|c/E/K L|T\W 77.8 7 4 6 3 0 dual b/y bias c:Cysteinylation 53.234371 0.936299 628.2689 1193.551 61.98 -2.1 1.1 4.25 2485.8 nuORFdb_human ENST00000367808.7_1_1:169337452-169337518:+ ENST00000367808.7_1_1:169337452-169337518:+ BLZF1 5' uORF 5' uORF GN=BLZF1 5' uORF E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep1_Slot1-04_1_3496 40mio-rep2 E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep2_Slot2-5_1_3520.15080.1320.2 2 1 1 14.97 -0.43 12.85 15.41 81.9 6 1 1 6800.0 C16c Hydrogen 13 (M) 9 EEVcEKLTW (-) 9 DLCEEVFGTAD E E|V|c/E|K L|T\W 55.6 5 5 5 4 0 dual b/y bias c:Cysteinylation 52.092793 0.926156 628.2684 1193.551 61.978 -3.7 1.2000000000000002 4.25 2485.8 nuORFdb_human ENST00000367808.7_1_1:169337452-169337518:+ ENST00000367808.7_1_1:169337452-169337518:+ BLZF1 5' uORF 5' uORF GN=BLZF1 5' uORF E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep2_Slot2-5_1_3520 40mio-rep3 E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep3_Slot2-5_1_3521.15086.1323.2 2 1 1 11.42 8.66 11.42 69.6 7 1 1 6780.0 C16c Hydrogen 13 (M) 9 EEVcEKLTW (-) 9 E E/V|c/E/K/L|T\W 77.8 7 3 6 2 0 y-ion bias c:Cysteinylation 52.114841 0.924449 628.2683 1193.551 61.978 -3.7 0.8999999999999995 4.25 2485.8 nuORFdb_human ENST00000367808.7_1_1:169337452-169337518:+ ENST00000367808.7_1_1:169337452-169337518:+ BLZF1 5' uORF 5' uORF GN=BLZF1 5' uORF E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep3_Slot2-5_1_3521 40mio-rep4 E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep4_Slot2-5_1_3522.15074.1323.2 2 1 1 7.86 0.29 8.27 7.57 67.7 5 1 1 10700.0 C16c Hydrogen 13 (M) 9 EEVcEKLTW (-) 9 EEKLEcSLW E E|V/c/E K L|T\W 44.4 4 3 4 2 0 unbiased c:Cysteinylation 52.058975 0.924741 628.2679 1193.551 61.978 -3.7 1.0 4.25 2485.8 nuORFdb_human ENST00000367808.7_1_1:169337452-169337518:+ ENST00000367808.7_1_1:169337452-169337518:+ BLZF1 5' uORF 5' uORF GN=BLZF1 5' uORF E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep4_Slot2-5_1_3522 5mio-rep1 E_20221201_NO30_400nL_HLAc1_5e6_directIP_titration_rep1_Slot1-37_1_3502.15478.1340.2 2 2 1 13.41 10.82 13.41 73.1 6 1 1 5880.0 C16c Hydrogen 13 (M) 9 EEVcEKLTW (-) 9 E E|V|c/E|K L|T\W 55.6 5 5 5 4 0 dual b/y bias c:Cysteinylation 53.466671 0.914008 628.263 1193.551 61.968 -11.6 -4.6 4.25 2485.8 nuORFdb_human ENST00000367808.7_1_1:169337452-169337518:+ ENST00000367808.7_1_1:169337452-169337518:+ BLZF1 5' uORF 5' uORF GN=BLZF1 5' uORF E_20221201_NO30_400nL_HLAc1_5e6_directIP_titration_rep1_Slot1-37_1_3502 20mio-rep1 E_20221201_NO30_400nL_HLAc1_2e7_directIP_titration_rep1_Slot2-4_1_3515.11249.1618.3 3 1 1 7.88 4.61 2.22 3.27 73.5 7 2 2 6080.0 Hydrogen 42 (E) 10 EEVLRDTRGW (T) 10 EEVLQnQRML E/E/V/L/R/D-T R\G W 60.0 6 1 5 0 1 y-ion bias 38.853584 0.73894 420.8818 1260.633 -0.0017 -1.3 4.9 4.68 8756.5 nuORFdb_human ENST00000369788.7_1_10:105209770-105211975:- ENST00000369788.7_1_10:105209770-105211975:-|ENST00000461631.1_1_10:105210516-105210915:- CALHM2 5' uORF 5' uORF GN=CALHM2 5' uORF E_20221201_NO30_400nL_HLAc1_2e7_directIP_titration_rep1_Slot2-4_1_3515 40mio-rep4 E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep4_Slot2-5_1_3522.11249.1612.3 3 1 1 9.8 6.09 3.71 3.71 80.8 6 2 2 6310.0 Hydrogen 42 (E) 10 EEVLRDTRGW (T) 10 IEnLQKEKEE E/E/V/L/R/D/T R G W 60.0 6 0 6 0 0 y-ion bias 38.848167 0.742441 420.881 1260.633 -0.0047 -3.7 1.0 4.68 8756.5 nuORFdb_human ENST00000369788.7_1_10:105209770-105211975:- ENST00000369788.7_1_10:105209770-105211975:-|ENST00000461631.1_1_10:105210516-105210915:- CALHM2 5' uORF 5' uORF GN=CALHM2 5' uORF E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep4_Slot2-5_1_3522 40mio-rep1 E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep1_Slot1-04_1_3496.21011.364.1 1 1 1 11.18 1.79 10.13 9.39 77.2 6 0 0 3050.0 Hydrogen 12 (R) 9 EEWQGPTAF (L) 9 EEWQESRT E E/W|Q|G|P T\A\F 55.6 5 5 4 3 0 dual b/y bias 72.582832 1.506703 1064.464 1064.468 -0.0084 -7.8 -4.6 3.8 7331.1 nuORFdb_human ENST00000375370.9_1_13:114286047-114288212:+ ENST00000375370.9_1_13:114286047-114288212:+ TFDP1 Out-of-Frame Out-of-Frame GN=TFDP1 Out-of-Frame E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep1_Slot1-04_1_3496 1mio-rep1 E_20221201_NO30_400nL_HLAc1_1e6_directIP_titration_rep1_Slot1-06_1_3498.7459.1465.2 2 1 1 8.27 3.1 3.49 5.17 55.7 6 1 1 2680.0 Hydrogen 11 (R) 9 EEYRGGNNW (-) 9 FGNnLDVnTE E E/Y/R/G G/N|N\W 55.6 5 2 5 1 0 y-ion bias 25.771338 0.835421 562.7378 1124.476 -0.0068 -6.1 -1.1999999999999993 4.53 2255.4 nuORFdb_human ENST00000358746.6_1_5:94886290-94886347:- ENST00000358746.6_1_5:94886290-94886347:- TTC37 5' uORF 5' uORF GN=TTC37 5' uORF E_20221201_NO30_400nL_HLAc1_1e6_directIP_titration_rep1_Slot1-06_1_3498 10mio-rep1 E_20221201_NO30_400nL_HLAc1_1e7_directIP_titration_rep1_Slot2-3_1_3512.7129.1476.2 2 2 1 8.46 3.43 5.16 5.03 60.4 6 1 1 30400.0 Hydrogen 11 (R) 9 EEYRGGNNW (-) 9 GAmATDKNDW E E/Y/R|G G/N\N\W 55.6 5 3 4 1 0 unbiased 24.63632 0.828771 562.738 1124.476 -0.0068 -6.1 1.6000000000000005 4.53 2255.4 nuORFdb_human ENST00000358746.6_1_5:94886290-94886347:- ENST00000358746.6_1_5:94886290-94886347:- TTC37 5' uORF 5' uORF GN=TTC37 5' uORF E_20221201_NO30_400nL_HLAc1_1e7_directIP_titration_rep1_Slot2-3_1_3512 10mio-rep2 E_20221201_NO30_400nL_HLAc1_1e7_directIP_titration_rep2_Slot2-3_1_3513.7079.1471.2 2 1 1 8.71 3.3 4.8 5.42 63.9 5 1 1 8810.0 Hydrogen 11 (R) 9 EEYRGGNNW (-) 9 GVEmLMENEG E E|Y R|G G\N|N\W 33.3 3 5 3 3 0 dual b/y bias 24.461826 0.831882 562.7371 1124.476 -0.0088 -7.9 -1.2000000000000002 4.53 2255.4 nuORFdb_human ENST00000358746.6_1_5:94886290-94886347:- ENST00000358746.6_1_5:94886290-94886347:- TTC37 5' uORF 5' uORF GN=TTC37 5' uORF E_20221201_NO30_400nL_HLAc1_1e7_directIP_titration_rep2_Slot2-3_1_3513 10mio-rep3 E_20221201_NO30_400nL_HLAc1_1e7_directIP_titration_rep3_Slot2-3_1_3514.7074.1477.2 2 3 1 9.78 3.41 5.68 6.36 66.5 7 1 1 22200.0 Hydrogen 11 (R) 9 EEYRGGNNW (-) 9 HMCTYLNW E E/Y/R\G/G\N|N\W 77.8 7 4 4 1 0 unbiased 24.445183 0.828026 562.7386 1124.476 -0.0048 -4.3 2.0 4.53 2255.4 nuORFdb_human ENST00000358746.6_1_5:94886290-94886347:- ENST00000358746.6_1_5:94886290-94886347:- TTC37 5' uORF 5' uORF GN=TTC37 5' uORF E_20221201_NO30_400nL_HLAc1_1e7_directIP_titration_rep3_Slot2-3_1_3514 20mio-rep1 E_20221201_NO30_400nL_HLAc1_2e7_directIP_titration_rep1_Slot2-4_1_3515.7031.1463.2 2 1 1 11.24 3.22 7.72 8.02 63.3 6 1 1 63500.0 Hydrogen 11 (R) 9 EEYRGGNNW (-) 9 GFGGPTCETAQ E E|Y/R|G G\N|N\W 55.6 5 5 4 3 0 dual b/y bias 24.291691 0.836558 562.7384 1124.476 -0.0068 -6.1 0.10000000000000053 4.53 2255.4 nuORFdb_human ENST00000358746.6_1_5:94886290-94886347:- ENST00000358746.6_1_5:94886290-94886347:- TTC37 5' uORF 5' uORF GN=TTC37 5' uORF E_20221201_NO30_400nL_HLAc1_2e7_directIP_titration_rep1_Slot2-4_1_3515 20mio-rep2 E_20221201_NO30_400nL_HLAc1_2e7_directIP_titration_rep3_Slot2-4_1_3518.7162.1473.2 2 2 1 7.99 0.73 6.36 7.26 60.9 5 1 1 17900.0 Hydrogen 11 (R) 9 EEYRGGNNW (-) 9 GVEmLMENEG E E|Y/R|G G N|N\W 44.4 4 4 4 3 0 dual b/y bias 24.744867 0.830331 562.7385 1124.476 -0.0048 -4.3 2.0 4.53 2255.4 nuORFdb_human ENST00000358746.6_1_5:94886290-94886347:- ENST00000358746.6_1_5:94886290-94886347:- TTC37 5' uORF 5' uORF GN=TTC37 5' uORF E_20221201_NO30_400nL_HLAc1_2e7_directIP_titration_rep3_Slot2-4_1_3518 20mio-rep3 E_20221201_NO30_400nL_HLAc1_2e7_directIP_titration_rep4_Slot2-4_1_3519.7064.1459.2 2 2 1 10.77 4.32 5.59 6.44 61.6 6 1 1 77200.0 Hydrogen 11 (R) 9 EEYRGGNNW (-) 9 GVEmLMENEG E E/Y/R|G G|N|N\W 55.6 5 4 5 3 0 dual b/y bias 24.403864 0.839283 562.7384 1124.476 -0.0068 -6.1 -0.2999999999999998 4.53 2255.4 nuORFdb_human ENST00000358746.6_1_5:94886290-94886347:- ENST00000358746.6_1_5:94886290-94886347:- TTC37 5' uORF 5' uORF GN=TTC37 5' uORF E_20221201_NO30_400nL_HLAc1_2e7_directIP_titration_rep4_Slot2-4_1_3519 40mio-rep1 E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep1_Slot1-04_1_3496.7317.1435.2 2 2 1 11.12 3.92 7.26 7.2 64.4 6 1 1 139000.0 Hydrogen 11 (R) 9 EEYRGGNNW (-) 9 FGNDNFGPQE E E|Y/R|G G\N|N\W 55.6 5 5 4 3 0 dual b/y bias 25.283131 0.854565 562.7394 1124.476 -0.0048 -4.3 -1.0999999999999996 4.53 2255.4 nuORFdb_human ENST00000358746.6_1_5:94886290-94886347:- ENST00000358746.6_1_5:94886290-94886347:- TTC37 5' uORF 5' uORF GN=TTC37 5' uORF E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep1_Slot1-04_1_3496 40mio-rep2 E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep2_Slot2-5_1_3520.7042.1442.2 2 2 1 12.83 4.05 8.3 8.78 66.9 7 1 1 166000.0 Hydrogen 11 (R) 9 EEYRGGNNW (-) 9 FGNDNFGPQE E/E|Y/R|G G\N|N\W 77.8 7 5 5 3 0 dual b/y bias 24.33239 0.850168 562.7385 1124.476 -0.0048 -4.3 0.6000000000000005 4.53 2255.4 nuORFdb_human ENST00000358746.6_1_5:94886290-94886347:- ENST00000358746.6_1_5:94886290-94886347:- TTC37 5' uORF 5' uORF GN=TTC37 5' uORF E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep2_Slot2-5_1_3520 40mio-rep3 E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep3_Slot2-5_1_3521.7038.1461.2 2 2 1 11.42 4.18 7.03 7.24 66.8 7 1 1 57900.0 Hydrogen 11 (R) 9 EEYRGGNNW (-) 9 FGNDNFGPQE E/E/Y/R|G G\N|N\W 77.8 7 4 5 2 0 unbiased 24.314915 0.838119 562.7376 1124.476 -0.0068 -6.1 -1.5 4.53 2255.4 nuORFdb_human ENST00000358746.6_1_5:94886290-94886347:- ENST00000358746.6_1_5:94886290-94886347:- TTC37 5' uORF 5' uORF GN=TTC37 5' uORF E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep3_Slot2-5_1_3521 40mio-rep4 E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep4_Slot2-5_1_3522.7021.1462.2 2 2 1 9.91 2.07 7.1 7.84 62.3 6 1 1 83900.0 Hydrogen 11 (R) 9 EEYRGGNNW (-) 9 YSPYGTHGGW E E/Y/R|G G\N|N\W 55.6 5 4 4 2 0 unbiased 24.251978 0.837396 562.7388 1124.476 -0.0048 -4.3 0.40000000000000036 4.53 2255.4 nuORFdb_human ENST00000358746.6_1_5:94886290-94886347:- ENST00000358746.6_1_5:94886290-94886347:- TTC37 5' uORF 5' uORF GN=TTC37 5' uORF E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep4_Slot2-5_1_3522 5mio-rep1 E_20221201_NO30_400nL_HLAc1_5e6_directIP_titration_rep1_Slot1-37_1_3502.7297.1467.2 2 2 1 8.99 3.87 5.01 5.12 60.7 6 1 1 13500.0 Hydrogen 11 (R) 9 EEYRGGNNW (-) 9 YSPYGTHGGW E E/Y/R|G\G N|N\W 55.6 5 4 4 2 0 unbiased 25.210962 0.834424 562.7386 1124.476 -0.0048 -4.3 2.7 4.53 2255.4 nuORFdb_human ENST00000358746.6_1_5:94886290-94886347:- ENST00000358746.6_1_5:94886290-94886347:- TTC37 5' uORF 5' uORF GN=TTC37 5' uORF E_20221201_NO30_400nL_HLAc1_5e6_directIP_titration_rep1_Slot1-37_1_3502 5mio-rep3 E_20221201_NO30_400nL_HLAc1_5e6_directIP_titration_rep3_Slot2-2_1_3511.7303.1473.2 2 1 1 8.15 4.86 4.2 3.29 61.2 6 1 1 4160.0 Hydrogen 11 (R) 9 EEYRGGNNW (-) 9 FGNDNFGPQE E E/Y/R|G\G N\N\W 55.6 5 4 3 1 0 unbiased 25.233471 0.830274 562.7364 1124.476 -0.0108 -9.6 -1.4000000000000004 4.53 2255.4 nuORFdb_human ENST00000358746.6_1_5:94886290-94886347:- ENST00000358746.6_1_5:94886290-94886347:- TTC37 5' uORF 5' uORF GN=TTC37 5' uORF E_20221201_NO30_400nL_HLAc1_5e6_directIP_titration_rep3_Slot2-2_1_3511 SCP E_20221201_NO30_400nL_HLAc1_4e7_PDAC_Slot2-6_1_3524.12555.384.1 1 3 1 13.36 7.76 5.95 5.6 85.7 8 1 1 24100.0 Hydrogen 17 (M) 9 EGLKETISY (C) 9 ETAVKELSY E/G/L/K|E\T\I\S\Y 100.0 9 5 4 1 0 unbiased 43.372974 1.494744 1039.5287 1039.531 -0.0006 -0.6 1.7999999999999998 4.53 4034.7 nuORFdb_human ENST00000403890.7_1_6:150067086-150067191:- ENST00000403890.7_1_6:150067086-150067191:- NUP43 Out-of-Frame Out-of-Frame GN=NUP43 Out-of-Frame E_20221201_NO30_400nL_HLAc1_4e7_PDAC_Slot2-6_1_3524 SCP E_20221201_NO30_400nL_HLAc1_4e7_PDAC_Slot2-6_1_3524.17604.1268.2 2 1 1 11.97 5.05 5.95 6.92 77.7 5 1 1 6320.0 Hydrogen 69 (Q) 11 EPFRDLSNNEF (S) 11 LSGNGPmPGLnFF E P F R D L|S\N|N\E\F 45.5 5 5 2 2 0 b-ion bias 60.816448 0.959001 684.3124 1367.623 -0.0059 -4.3 -1.9 4.14 9820.0 nuORFdb_human ENST00000588221.5_1_19:9545835-9546183:- ENST00000588221.5_1_19:9545835-9546183:- ZNF266 5' uORF 5' uORF GN=ZNF266 5' uORF E_20221201_NO30_400nL_HLAc1_4e7_PDAC_Slot2-6_1_3524 Exploris_FAIMS VH20221207_MKP_FAIMS2022E_HLAc1_4e7_PDAC.43195.43195.2 2 1 1 10.51 4.41 7.39 6.1 74.0 6 1 1 1440000.0 Hydrogen 45 (G) 9 EPVTLRAAF (G) 9 EPVFVVTGR E P/V/T/L/R-A A/F 55.6 5 0 5 0 1 y-ion bias 0.958 89.4 78.58 120.0 -70.0 502.2811 1003.557 -0.0023 -2.3 0.30000000000000027 6.1 6863.9 nuORFdb_human ENST00000302513.6_1_2:99758144-99758336:+ ENST00000302513.6_1_2:99758144-99758336:+ C2orf15 5' uORF 5' uORF GN=C2orf15 5' uORF VH20221207_MKP_FAIMS2022E_HLAc1_4e7_PDAC 20mio-rep3 E_20221201_NO30_400nL_HLAc1_2e7_directIP_titration_rep4_Slot2-4_1_3519.29113.307.1 1 1 1 7.76 6.28 0.35 1.47 64.6 6 0 0 3200.0 Hydrogen 6 (L) 9 EQVILGLLL (L) 9 LGLTPTGILI E Q V\I\L\G\L\L\L 66.7 6 6 0 0 0 b-ion bias 100.552971 1.540497 997.6275 997.629 -0.0012 -1.2 4.6 4.0 2408.1 nuORFdb_human T331461_7:113948927-113957058:- T331461_7:113948927-113957058:- lincRNA lncRNA GN=nan lincRNA E_20221201_NO30_400nL_HLAc1_2e7_directIP_titration_rep4_Slot2-4_1_3519 1mio-rep1 E_20221201_NO30_400nL_HLAc1_1e6_directIP_titration_rep1_Slot1-06_1_3498.15938.388.1 1 2 1 10.3 2.51 2.64 7.79 76.5 6 0 0 3730.0 Hydrogen 41 (D) 10 ESDVSSALSY (I) 10 LnITGSnSSY E S D V|S/S\A\L\S\Y 60.0 6 5 2 1 0 b-ion bias 55.048164 1.492256 1057.4558 1057.468 -0.0084 -8.0 -3.0999999999999996 3.67 28843.8 smORF_v4 LRRC37BP1_17:28958817-28961287:+ LRRC37BP1_17:28958817-28961287:+ LRRC37BP1 Pseudogene 17:28958817-28961287:+ 17 Pseudogene GN=LRRC37BP1 E_20221201_NO30_400nL_HLAc1_1e6_directIP_titration_rep1_Slot1-06_1_3498 1mio-rep2 E_20221201_NO30_400nL_HLAc1_1e6_directIP_titration_rep3_Slot2-1_1_3508.15885.417.1 1 1 1 7.69 2.98 1.87 4.71 68.0 6 0 0 2080.0 Hydrogen 41 (D) 10 ESDVSSALSY (I) 10 NGCDKTISY E S D V|S/S\A\L\S\Y 60.0 6 5 2 1 0 b-ion bias 54.865074 1.475119 1057.4589 1057.468 -0.0084 -8.0 0.3000000000000007 3.67 28843.8 smORF_v4 LRRC37BP1_17:28958817-28961287:+ LRRC37BP1_17:28958817-28961287:+ LRRC37BP1 Pseudogene 17:28958817-28961287:+ 17 Pseudogene GN=LRRC37BP1 E_20221201_NO30_400nL_HLAc1_1e6_directIP_titration_rep3_Slot2-1_1_3508 1mio-rep3 E_20221201_NO30_400nL_HLAc1_1e6_directIP_titration_rep4_Slot2-1_1_3509.15808.412.1 1 1 1 9.41 2.32 1.5 7.09 72.3 6 0 0 1940.0 Hydrogen 41 (D) 10 ESDVSSALSY (I) 10 KDTEDSLSY E S D V|S\S\A\L\S\Y 60.0 6 6 1 1 0 b-ion bias 54.592922 1.478487 1057.4584 1057.468 -0.0084 -8.0 0.0 3.67 28843.8 smORF_v4 LRRC37BP1_17:28958817-28961287:+ LRRC37BP1_17:28958817-28961287:+ LRRC37BP1 Pseudogene 17:28958817-28961287:+ 17 Pseudogene GN=LRRC37BP1 E_20221201_NO30_400nL_HLAc1_1e6_directIP_titration_rep4_Slot2-1_1_3509 10mio-rep1 E_20221201_NO30_400nL_HLAc1_1e7_directIP_titration_rep1_Slot2-3_1_3512.15602.402.1 1 3 1 11.32 2.76 2.7 8.56 74.9 7 0 0 11900.0 Hydrogen 41 (D) 10 ESDVSSALSY (I) 10 NGCDKTISY E S D/V|S|S\A\L\S\Y 70.0 7 6 3 2 0 b-ion bias 53.898151 1.484413 1057.4622 1057.468 -0.0084 -8.0 -0.2999999999999998 3.67 28843.8 smORF_v4 LRRC37BP1_17:28958817-28961287:+ LRRC37BP1_17:28958817-28961287:+ LRRC37BP1 Pseudogene 17:28958817-28961287:+ 17 Pseudogene GN=LRRC37BP1 E_20221201_NO30_400nL_HLAc1_1e7_directIP_titration_rep1_Slot2-3_1_3512 10mio-rep2 E_20221201_NO30_400nL_HLAc1_1e7_directIP_titration_rep2_Slot2-3_1_3513.15547.404.1 1 1 1 9.48 3.45 0.89 6.03 73.7 6 0 0 5770.0 Hydrogen 41 (D) 10 ESDVSSALSY (I) 10 KDTEDSLSY E S D V|S\S\A\L\S\Y 60.0 6 6 1 1 0 b-ion bias 53.705257 1.482965 1057.4612 1057.468 -0.0084 -8.0 -1.2999999999999998 3.67 28843.8 smORF_v4 LRRC37BP1_17:28958817-28961287:+ LRRC37BP1_17:28958817-28961287:+ LRRC37BP1 Pseudogene 17:28958817-28961287:+ 17 Pseudogene GN=LRRC37BP1 E_20221201_NO30_400nL_HLAc1_1e7_directIP_titration_rep2_Slot2-3_1_3513 10mio-rep3 E_20221201_NO30_400nL_HLAc1_1e7_directIP_titration_rep3_Slot2-3_1_3514.15503.412.1 1 3 1 10.6 4.32 2.97 6.28 77.4 7 0 0 19800.0 Hydrogen 41 (D) 10 ESDVSSALSY (I) 10 LKSEEDSSY E S D\V|S\S\A\L\S\Y 70.0 7 7 1 1 0 b-ion bias 53.556129 1.47825 1057.4623 1057.468 -0.0084 -8.0 -1.7000000000000002 3.67 28843.8 smORF_v4 LRRC37BP1_17:28958817-28961287:+ LRRC37BP1_17:28958817-28961287:+ LRRC37BP1 Pseudogene 17:28958817-28961287:+ 17 Pseudogene GN=LRRC37BP1 E_20221201_NO30_400nL_HLAc1_1e7_directIP_titration_rep3_Slot2-3_1_3514 20mio-rep1 E_20221201_NO30_400nL_HLAc1_2e7_directIP_titration_rep1_Slot2-4_1_3515.15490.405.1 1 5 1 12.57 4.01 1.78 8.55 77.6 6 0 0 87000.0 Hydrogen 41 (D) 10 ESDVSSALSY (I) 10 KDTEDSLSY E S D V|S|S\A\L\S\Y 60.0 6 6 2 2 0 b-ion bias 53.497795 1.482384 1057.4637 1057.468 -0.0084 -8.0 -1.7999999999999998 3.67 28843.8 smORF_v4 LRRC37BP1_17:28958817-28961287:+ LRRC37BP1_17:28958817-28961287:+ LRRC37BP1 Pseudogene 17:28958817-28961287:+ 17 Pseudogene GN=LRRC37BP1 E_20221201_NO30_400nL_HLAc1_2e7_directIP_titration_rep1_Slot2-4_1_3515 20mio-rep2 E_20221201_NO30_400nL_HLAc1_2e7_directIP_titration_rep3_Slot2-4_1_3518.15569.401.1 1 4 1 11.14 2.6 2.16 8.54 76.7 6 0 0 11900.0 Hydrogen 41 (D) 10 ESDVSSALSY (I) 10 VSSCQNLSY E S D V|S|S\A\L\S\Y 60.0 6 6 2 2 0 b-ion bias 53.775215 1.484436 1057.4628 1057.468 -0.0084 -8.0 -1.7000000000000002 3.67 28843.8 smORF_v4 LRRC37BP1_17:28958817-28961287:+ LRRC37BP1_17:28958817-28961287:+ LRRC37BP1 Pseudogene 17:28958817-28961287:+ 17 Pseudogene GN=LRRC37BP1 E_20221201_NO30_400nL_HLAc1_2e7_directIP_titration_rep3_Slot2-4_1_3518 20mio-rep3 E_20221201_NO30_400nL_HLAc1_2e7_directIP_titration_rep4_Slot2-4_1_3519.15512.1482.2 2 4 1 12.3 4.38 1.09 7.93 68.5 6 0 0 48000.0 Hydrogen 41 (D) 10 ESDVSSALSY (I) 10 KDTEDSLSY E S D V|S|S\A|L|S\Y 60.0 6 6 4 4 0 dual b/y bias 53.580128 0.824555 529.2345 1057.468 -0.0057 -5.4 0.39999999999999947 3.67 28843.8 smORF_v4 LRRC37BP1_17:28958817-28961287:+ LRRC37BP1_17:28958817-28961287:+ LRRC37BP1 Pseudogene 17:28958817-28961287:+ 17 Pseudogene GN=LRRC37BP1 E_20221201_NO30_400nL_HLAc1_2e7_directIP_titration_rep4_Slot2-4_1_3519 40mio-rep1 E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep1_Slot1-04_1_3496.15787.377.1 1 4 1 12.97 3.24 2.09 9.72 82.1 6 0 0 177000.0 Hydrogen 41 (D) 10 ESDVSSALSY (I) 10 KDTEDSLSY E S D V|S|S\A\L\S\Y 60.0 6 6 2 2 0 b-ion bias 54.533344 1.499019 1057.4651 1057.468 0.0016 1.5 4.7 3.67 28843.8 smORF_v4 LRRC37BP1_17:28958817-28961287:+ LRRC37BP1_17:28958817-28961287:+ LRRC37BP1 Pseudogene 17:28958817-28961287:+ 17 Pseudogene GN=LRRC37BP1 E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep1_Slot1-04_1_3496 40mio-rep2 E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep2_Slot2-5_1_3520.15457.1475.2 2 3 1 14.45 6.33 2.28 8.12 82.1 6 0 0 62200.0 Hydrogen 41 (D) 10 ESDVSSALSY (I) 10 KDTEDSLSY E S D V/S|S|A|L|S\Y 60.0 6 5 5 4 0 dual b/y bias 53.395126 0.829135 529.2358 1057.468 -0.0037 -3.5 1.4000000000000004 3.67 28843.8 smORF_v4 LRRC37BP1_17:28958817-28961287:+ LRRC37BP1_17:28958817-28961287:+ LRRC37BP1 Pseudogene 17:28958817-28961287:+ 17 Pseudogene GN=LRRC37BP1 E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep2_Slot2-5_1_3520 40mio-rep3 E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep3_Slot2-5_1_3521.15457.1479.2 2 3 1 16.96 7.72 2.05 9.24 87.2 6 0 0 47000.0 Hydrogen 41 (D) 10 ESDVSSALSY (I) 10 KDTEDSLSY E S D V|S|S|A|L|S\Y 60.0 6 6 5 5 0 dual b/y bias 53.395592 0.826515 529.2353 1057.468 -0.0057 -5.4 -0.8000000000000007 3.67 28843.8 smORF_v4 LRRC37BP1_17:28958817-28961287:+ LRRC37BP1_17:28958817-28961287:+ LRRC37BP1 Pseudogene 17:28958817-28961287:+ 17 Pseudogene GN=LRRC37BP1 E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep3_Slot2-5_1_3521 40mio-rep4 E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep4_Slot2-5_1_3522.15458.1478.2 2 4 1 16.79 7.23 2.85 9.56 85.6 6 0 0 114000.0 Hydrogen 41 (D) 10 ESDVSSALSY (I) 10 VSSCQNLSY E S D V|S|S|A|L|S\Y 60.0 6 6 5 5 0 dual b/y bias 53.384537 0.827439 529.2357 1057.468 -0.0037 -3.5 1.2000000000000002 3.67 28843.8 smORF_v4 LRRC37BP1_17:28958817-28961287:+ LRRC37BP1_17:28958817-28961287:+ LRRC37BP1 Pseudogene 17:28958817-28961287:+ 17 Pseudogene GN=LRRC37BP1 E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep4_Slot2-5_1_3522 5mio-rep2 E_20221201_NO30_400nL_HLAc1_5e6_directIP_titration_rep2_Slot2-2_1_3510.15767.416.1 1 2 1 10.89 3.67 1.68 7.22 75.0 6 0 0 7390.0 Hydrogen 41 (D) 10 ESDVSSALSY (I) 10 VSSCQNLSY E S D V/S|S\A\L\S\Y 60.0 6 5 2 1 0 b-ion bias 54.45937 1.475994 1057.4565 1057.468 -0.0084 -8.0 0.6999999999999993 3.67 28843.8 smORF_v4 LRRC37BP1_17:28958817-28961287:+ LRRC37BP1_17:28958817-28961287:+ LRRC37BP1 Pseudogene 17:28958817-28961287:+ 17 Pseudogene GN=LRRC37BP1 E_20221201_NO30_400nL_HLAc1_5e6_directIP_titration_rep2_Slot2-2_1_3510 5mio-rep3 E_20221201_NO30_400nL_HLAc1_5e6_directIP_titration_rep3_Slot2-2_1_3511.15762.413.1 1 1 1 10.63 3.39 0.74 7.25 74.9 6 0 0 2400.0 Hydrogen 41 (D) 10 ESDVSSALSY (I) 10 VSSCQNLSY E S D V|S|S\A\L\S\Y 60.0 6 6 2 2 0 b-ion bias 54.444389 1.477809 1057.4585 1057.468 -0.0084 -8.0 0.1999999999999993 3.67 28843.8 smORF_v4 LRRC37BP1_17:28958817-28961287:+ LRRC37BP1_17:28958817-28961287:+ LRRC37BP1 Pseudogene 17:28958817-28961287:+ 17 Pseudogene GN=LRRC37BP1 E_20221201_NO30_400nL_HLAc1_5e6_directIP_titration_rep3_Slot2-2_1_3511 40mio-rep1 E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep1_Slot1-04_1_3496.21053.1305.2 2 1 1 12.78 6.91 6.8 5.86 78.2 6 1 1 4100.0 Hydrogen 5 (H) 9 ESLQVYRYW (Y) 9 nTIQVHTFPEG E S/L/Q|V|Y|R|Y W 55.6 5 4 6 4 0 dual b/y bias 72.727859 0.935474 622.3088 1243.611 0.0001 0.1 3.3000000000000003 6.1 2274.5 nuORFdb_human ENST00000630259.1_2_8:125535224-125551297:- ENST00000630259.1_2_8:125535224-125551297:- TATDN1 5' Overlap uORF 5' Overlap uORF GN=TATDN1 5' Overlap uORF E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep1_Slot1-04_1_3496 40mio-rep4 E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep4_Slot2-5_1_3522.5765.1447.2 2 1 1 10.76 3.58 3.0 7.17 75.3 7 1 1 4050.0 Hydrogen 36 (K) 10 ETDGAQVGRY (A) 10 ETDQNIRYG E T/D/G/A/Q|V|G\R Y 60.0 6 3 6 2 0 y-ion bias 19.916445 0.846906 548.2546 1095.506 -0.0038 -3.5 1.2000000000000002 4.37 8093.1 nuORFdb_human ENST00000484445.5_1_1:40915787-40916492:+ ENST00000484445.5_1_1:40915787-40916492:+|ENST00000469416.1_1_1:40916330-40916492:+ ZFP69B 5' uORF 5' uORF GN=ZFP69B 5' uORF E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep4_Slot2-5_1_3522 40mio-rep1 E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep1_Slot1-04_1_3496.13091.1284.2 2 1 1 9.07 6.56 4.51 2.5 67.5 7 1 1 7230.0 Hydrogen 8 (L) 10 ETDIEMETRY (K) 10 MHLTSEELnPn E T/D/I|E|M/E/T/R Y 60.0 6 2 7 2 0 y-ion bias 45.220985 0.948542 643.7801 1286.557 -0.0042 -3.3 -0.09999999999999964 4.0 2466.8 nuORFdb_human ENST00000524003.1_1_8:92081945-92082008:- ENST00000524003.1_1_8:92081945-92082008:- OTUD6B-AS1 Antisense lncRNA GN=OTUD6B-AS1 Antisense E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep1_Slot1-04_1_3496 20mio-rep1 E_20221201_NO30_400nL_HLAc1_2e7_directIP_titration_rep1_Slot2-4_1_3515.9624.1343.2 2 2 1 11.42 6.08 8.41 5.34 68.8 8 1 1 6820.0 M13m Hydrogen 8 (L) 10 ETDIEmETRY (K) 10 mHLTSEELnPn E T/D\I/E/m/E/T/R\Y 80.0 8 2 6 0 0 y-ion bias m:Oxidized methionine 33.243462 0.911721 651.7749 1286.557 15.9858 -7.0 -0.7999999999999998 4.0 2466.8 nuORFdb_human ENST00000524003.1_1_8:92081945-92082008:- ENST00000524003.1_1_8:92081945-92082008:- OTUD6B-AS1 Antisense lncRNA GN=OTUD6B-AS1 Antisense E_20221201_NO30_400nL_HLAc1_2e7_directIP_titration_rep1_Slot2-4_1_3515 40mio-rep2 E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep2_Slot2-5_1_3520.9621.1330.2 2 2 1 14.94 8.53 7.46 6.41 74.8 8 1 1 9060.0 M13m Hydrogen 8 (L) 10 ETDIEmETRY (K) 10 mHLTSEELnPn E T/D|I|E/m/E|T/R\Y 80.0 8 4 7 3 0 unbiased m:Oxidized methionine 33.236794 0.92043 651.776 1286.557 15.9878 -5.5 -0.5999999999999996 4.0 2466.8 nuORFdb_human ENST00000524003.1_1_8:92081945-92082008:- ENST00000524003.1_1_8:92081945-92082008:- OTUD6B-AS1 Antisense lncRNA GN=OTUD6B-AS1 Antisense E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep2_Slot2-5_1_3520 40mio-rep3 E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep3_Slot2-5_1_3521.9615.1329.2 2 3 1 11.59 5.91 7.51 5.68 71.8 8 1 1 11200.0 M13m Hydrogen 8 (L) 10 ETDIEmETRY (K) 10 mHLTSEELnPn E T/D|I|E/m/E/T/R\Y 80.0 8 3 7 2 0 y-ion bias m:Oxidized methionine 33.217876 0.920909 651.7768 1286.557 15.9898 -3.9 0.6999999999999997 4.0 2466.8 nuORFdb_human ENST00000524003.1_1_8:92081945-92082008:- ENST00000524003.1_1_8:92081945-92082008:- OTUD6B-AS1 Antisense lncRNA GN=OTUD6B-AS1 Antisense E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep3_Slot2-5_1_3521 20mio-rep2 E_20221201_NO30_400nL_HLAc1_2e7_directIP_titration_rep3_Slot2-4_1_3518.9656.1682.3 3 2 1 7.92 3.1 3.36 4.82 67.2 6 2 2 7760.0 Hydrogen 43 (E) 9 EVLRDTRGW (T) 9 LDLRSTGICP E V/L/R/D/T/R/G W 55.6 5 0 6 0 0 y-ion bias 33.355587 0.698304 377.865 1131.591 -0.0101 -8.9 -2.6000000000000005 6.17 8756.5 nuORFdb_human ENST00000369788.7_1_10:105209770-105211975:- ENST00000369788.7_1_10:105209770-105211975:-|ENST00000461631.1_1_10:105210516-105210915:- CALHM2 5' uORF 5' uORF GN=CALHM2 5' uORF E_20221201_NO30_400nL_HLAc1_2e7_directIP_titration_rep3_Slot2-4_1_3518 40mio-rep1 E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep1_Slot1-04_1_3496.9825.1362.2 2 1 1 8.09 5.18 3.33 2.91 78.9 5 2 2 74200.0 Hydrogen 43 (E) 9 EVLRDTRGW (T) 9 GGIGGGTITRGW E/V L R/D|T R\G\W 44.4 4 3 3 1 0 unbiased 33.942307 0.900312 566.2968 1131.591 -0.0038 -3.4 -0.19999999999999973 6.17 8756.5 nuORFdb_human ENST00000369788.7_1_10:105209770-105211975:- ENST00000369788.7_1_10:105209770-105211975:-|ENST00000461631.1_1_10:105210516-105210915:- CALHM2 5' uORF 5' uORF GN=CALHM2 5' uORF E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep1_Slot1-04_1_3496 Eva-50mio-3fr AL20210827_EV_HLA_PDAC_PANFRO413_2D_50e6_W632_classI_fxn2.42285.42398.2 2 2 2 7.23 2.45 3.2 4.79 58.8 6 0 0 3180000.0 Hydrogen 177 (T) 8 EVPAPLAP (-) 8 qPVIPASV E/V|P|A-P-L A-P 75.0 6 2 3 2 3 internal-ion bias 0.946 88.0 77.32 100.0 0.0 397.2261 793.445 -0.0006 -0.8 -1.1 4.0 19427.2 nuORFdb_human ENST00000370220.1_1_10:102759899-102760451:+ ENST00000370220.1_1_10:102759899-102760451:+ LZTS2 5' uORF 5' uORF GN=LZTS2 5' uORF AL20210827_EV_HLA_PDAC_PANFRO413_2D_50e6_W632_classI_fxn2 SCP E_20221201_NO30_400nL_HLAc1_4e7_PDAC_Slot2-6_1_3524.24375.1229.2 2 2 1 9.8 9.8 9.8 51.3 6 1 1 16200.0 Hydrogen 20 (A) 11 EYLCQMKGLTF (F) 11 E Y|L|C/Q M/K G L|T\F 36.4 4 4 5 3 0 unbiased Carbamidomethylation C:Carbamidomethylation, 84.208839 0.982758 695.3279 1389.654 -0.0054 -3.9 -1.5 6.1 5206.1 nuORFdb_human ENST00000428402.6_1_2:175087868-175094150:- ENST00000428402.6_1_2:175087868-175094150:- OLA1 Out-of-Frame Out-of-Frame GN=OLA1 Out-of-Frame E_20221201_NO30_400nL_HLAc1_4e7_PDAC_Slot2-6_1_3524 SCP E_20221201_NO30_400nL_HLAc1_4e7_PDAC_Slot2-6_1_3524.17823.1215.2 2 3 1 15.8 7.25 10.93 8.55 85.6 7 1 1 25300.0 M25m Hydrogen 20 (A) 11 EYLCQmKGLTF (F) 11 YLEcIERITF E Y|L|C/Q/m/K G L|T\F 54.5 6 4 6 3 0 unbiased Carbamidomethylation C:Carbamidomethylation,m:Oxidized methionine 61.573452 0.991873 703.3253 1389.654 15.9886 -4.5 -2.1 6.1 5206.1 nuORFdb_human ENST00000428402.6_1_2:175087868-175094150:- ENST00000428402.6_1_2:175087868-175094150:- OLA1 Out-of-Frame Out-of-Frame GN=OLA1 Out-of-Frame E_20221201_NO30_400nL_HLAc1_4e7_PDAC_Slot2-6_1_3524 SCP E_20221201_NO30_400nL_HLAc1_4e7_PDAC_Slot2-6_1_3524.24867.317.1 1 2 1 9.82 2.51 9.1 7.31 73.1 6 0 0 17600.0 Hydrogen 44 (T) 9 EYLSLTNGF (R) 9 LYELSASNF E Y/L|S|L|T\N G\F 55.6 5 5 4 3 0 dual b/y bias 85.908432 1.534312 1043.5042 1043.504 -0.0044 -4.2 -1.8000000000000003 4.0 8669.0 nuORFdb_human ENST00000361198.9_1_10:103870664-103871239:- ENST00000361198.9_1_10:103870664-103871239:- LDB1 5' Overlap uORF 5' Overlap uORF GN=LDB1 5' Overlap uORF E_20221201_NO30_400nL_HLAc1_4e7_PDAC_Slot2-6_1_3524 Eva-50mio-3fr AL20210827_EV_HLA_PDAC_PANFRO413_2D_50e6_W632_classI_fxn2.34745.34745.2 2 1 1 10.72 4.56 10.74 6.16 65.0 7 0 0 8040000.0 Hydrogen 2 (M) 9 EYNNIFHTF (T) 9 VEYTCKHFT E Y|N/N-I/F/H/T/F 77.8 7 1 6 1 1 y-ion bias 0.959 79.8 63.75 100.0 0.0 592.7731 1184.537 0.0018 1.5 1.2 5.24 5604.2 nuORFdb_human ENST00000545363.6_1_12:9808558-9809281:+ ENST00000545363.6_1_12:9808558-9809281:+|ENST00000633848.1_1_12:9808558-9809555:+ RP11-705C15.2 Pseudogene Pseudogene GN=RP11-705C15.2 Pseudogene AL20210827_EV_HLA_PDAC_PANFRO413_2D_50e6_W632_classI_fxn2 Eva-50mio-3fr AL20210827_EV_HLA_PDAC_PANFRO413_2D_50e6_W632_classI_fxn2.33866.33971.2 2 2 2 11.97 6.59 6.42 5.38 84.0 6 1 1 82300000.0 Hydrogen 19 (A) 9 EYPELRGVF (-) 9 nPAIRVFEY E Y|P/E/L/R-G V/F 55.6 5 1 5 1 1 y-ion bias 0.919 85.8 62.3 100.0 0.0 555.2854 1109.563 0.0009 0.8 0.5 4.53 2951.4 smORF_v4|nuORFdb_human GEMIN6_2:39005337-39005419:+ GEMIN6_2:39005337-39005419:+|ENST00000409011.5_2_2:39005336-39005417:+ GEMIN6 uORF 2:39005337-39005419:+ 2 GEMIN6 5' uORF 5' uORF GN=GEMIN6 AL20210827_EV_HLA_PDAC_PANFRO413_2D_50e6_W632_classI_fxn2 Exploris AL20221201_MKP_PDAC_01.44149.44149.2 2 2 1 10.44 5.47 4.21 4.97 81.7 5 1 1 23000000.0 Hydrogen 19 (A) 9 EYPELRGVF (-) 9 EYPFRVGEL E Y|P/E/L/R G V/F 44.4 4 1 5 1 0 y-ion bias 0.759 88.6 67.53 4.69 0.0 555.2858 1109.563 0.0017 1.5 0.7 4.53 2951.4 smORF_v4|nuORFdb_human GEMIN6_2:39005337-39005419:+ GEMIN6_2:39005337-39005419:+|ENST00000409011.5_2_2:39005336-39005417:+ GEMIN6 uORF 2:39005337-39005419:+ 2 GEMIN6 5' uORF 5' uORF GN=GEMIN6 AL20221201_MKP_PDAC_01 Exploris_FAIMS VH20221207_MKP_FAIMS2022E_HLAc1_4e7_PDAC.53571.53571.2 2 4 1 8.74 3.44 5.45 5.3 69.6 5 1 1 283000000.0 Hydrogen 19 (A) 9 EYPELRGVF (-) 9 YERDPGLLF E Y/P/E/L/R G V/F 44.4 4 0 5 0 0 y-ion bias 0.763 65.7 93.55 15.93 -50.0 555.2828 1109.563 -0.0043 -3.8 -1.1999999999999997 4.53 2951.4 smORF_v4|nuORFdb_human GEMIN6_2:39005337-39005419:+ GEMIN6_2:39005337-39005419:+|ENST00000409011.5_2_2:39005336-39005417:+ GEMIN6 uORF 2:39005337-39005419:+ 2 GEMIN6 5' uORF 5' uORF GN=GEMIN6 VH20221207_MKP_FAIMS2022E_HLAc1_4e7_PDAC SCP E_20221201_NO30_400nL_HLAc1_4e7_PDAC_Slot2-6_1_3524.7179.1408.2 2 2 1 10.52 3.96 7.07 6.56 70.4 6 1 1 12200.0 Hydrogen 34 (S) 9 EYTQKQATF (G) 9 EKSNYVGATF E Y/T|Q/K Q\A|T\F 55.6 5 4 4 2 0 unbiased 24.805426 0.871595 558.2703 1115.537 -0.004 -3.6 -1.2000000000000002 6.1 5240.0 nuORFdb_human ENST00000357103.4_1_12:1887050-1887191:+ ENST00000357103.4_1_12:1887050-1887191:+ ADIPOR2 Out-of-Frame Out-of-Frame GN=ADIPOR2 Out-of-Frame E_20221201_NO30_400nL_HLAc1_4e7_PDAC_Slot2-6_1_3524 SCP E_20221201_NO30_400nL_HLAc1_4e7_PDAC_Slot2-6_1_3524.28059.186.1 1 1 1 11.49 1.82 4.88 9.67 78.5 6 1 1 7060.0 Hydrogen 16 (K) 9 EYTSIFIKF (I) 9 nGPVVGnTLKF E Y T/S|I|F|I\K\F 66.7 6 5 4 3 0 dual b/y bias 96.939217 1.611238 1147.5994 1147.603 -0.0034 -3.0 -0.6000000000000001 6.1 5508.5 nuORFdb_human ENST00000393766.6_1_4:166259844-166261421:+ ENST00000393766.6_1_4:166259844-166261421:+ MSMO1 Out-of-Frame Out-of-Frame GN=MSMO1 Out-of-Frame E_20221201_NO30_400nL_HLAc1_4e7_PDAC_Slot2-6_1_3524 SCP E_20221201_NO30_400nL_HLAc1_4e7_PDAC_Slot2-6_1_3524.17065.617.1 1 1 1 8.27 3.29 3.55 4.98 76.6 5 0 0 13500.0 Hydrogen 41 (V) 9 FAAATAVAM (R) 9 FNSLVAAM F A A A\T\A\V\A\M 55.6 5 5 0 0 0 b-ion bias 58.954685 1.355947 852.4256 852.428 -0.0024 -2.8 -0.3999999999999999 5.52 5462.4 smORF_v4|nuORFdb_human SNX13_7:17979948-17980106:- SNX13_7:17979948-17980106:-|ENST00000428135.7_1_7:17979950-17980106:- SNX13 uORF 7:17979948-17980106:- 7 SNX13 5' uORF 5' uORF GN=SNX13 E_20221201_NO30_400nL_HLAc1_4e7_PDAC_Slot2-6_1_3524 SCP E_20221201_NO30_400nL_HLAc1_4e7_PDAC_Slot2-6_1_3524.11512.643.1 1 1 1 8.05 2.05 3.63 6.0 51.4 5 0 0 6870.0 M49m Hydrogen 41 (V) 9 FAAATAVAm (R) 9 GLGFSGLAm F A A A|T/A|V\A\m 55.6 5 4 3 2 0 unbiased m:Oxidized methionine 39.771343 1.340273 868.4208 852.428 15.9926 -2.7 -0.30000000000000027 5.52 5462.4 smORF_v4|nuORFdb_human SNX13_7:17979948-17980106:- SNX13_7:17979948-17980106:-|ENST00000428135.7_1_7:17979950-17980106:- SNX13 uORF 7:17979948-17980106:- 7 SNX13 5' uORF 5' uORF GN=SNX13 E_20221201_NO30_400nL_HLAc1_4e7_PDAC_Slot2-6_1_3524 40mio-rep1 E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep1_Slot1-04_1_3496.15242.1445.2 2 1 1 10.93 7.55 3.22 3.39 73.9 6 1 1 11400.0 Hydrogen 43 (V) 9 FAFGRGQSL (L) 9 FAKKECSI F/A/F|G R\G-Q-S\L 77.8 7 3 3 1 2 unbiased 52.649914 0.848225 491.7573 982.51 -0.0038 -3.8 -0.5999999999999996 9.75 5836.8 nuORFdb_human ENST00000575379.1_1_17:7358950-7359124:+ ENST00000575379.1_1_17:7358950-7359124:+ CHRNB1 5' uORF 5' uORF GN=CHRNB1 5' uORF E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep1_Slot1-04_1_3496 40mio-rep2 E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep2_Slot2-5_1_3520.14928.1460.2 2 1 1 9.52 6.76 2.03 2.76 63.9 7 1 1 6090.0 Hydrogen 43 (V) 9 FAFGRGQSL (L) 9 FALFPKEM F/A/F/G/R\G Q-S\L 77.8 7 2 4 0 1 y-ion bias 51.567013 0.838859 491.7568 982.51 -0.0038 -3.8 1.1000000000000005 9.75 5836.8 nuORFdb_human ENST00000575379.1_1_17:7358950-7359124:+ ENST00000575379.1_1_17:7358950-7359124:+ CHRNB1 5' uORF 5' uORF GN=CHRNB1 5' uORF E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep2_Slot2-5_1_3520 40mio-rep4 E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep4_Slot2-5_1_3522.14959.1464.2 2 1 1 7.36 5.43 2.27 1.93 74.5 5 1 1 8960.0 Hydrogen 43 (V) 9 FAFGRGQSL (L) 9 FAKKECSI F/A/F/G R\G Q S\L 44.4 4 2 3 0 0 unbiased 51.662022 0.836161 491.7577 982.51 -0.0018 -1.8 2.9000000000000004 9.75 5836.8 nuORFdb_human ENST00000575379.1_1_17:7358950-7359124:+ ENST00000575379.1_1_17:7358950-7359124:+ CHRNB1 5' uORF 5' uORF GN=CHRNB1 5' uORF E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep4_Slot2-5_1_3522 Exploris_FAIMS VH20221207_MKP_FAIMS2022E_HLAc1_4e7_PDAC.25249.25249.2 2 2 1 9.9 3.52 2.23 6.38 73.4 5 0 0 194000000.0 Hydrogen 245 (Q) 8 FAHSAVEM (A) 8 ETHFQVM F A H\S-A\V\E/M 62.5 5 3 1 0 1 b-ion bias 0.993 99.6 51.63 5.49 -50.0 446.204 891.403 -0.0021 -2.4 0.20000000000000018 5.24 61769.9 nuORFdb_human ENST00000370082.1_1_20:62201547-62204491:- ENST00000370082.1_1_20:62201547-62204491:-|ENST00000479540.5_1_20:62203456-62204512:- HELZ2 5' Overlap uORF 5' Overlap uORF GN=HELZ2 5' Overlap uORF VH20221207_MKP_FAIMS2022E_HLAc1_4e7_PDAC Eva-50mio-3fr AL20210827_EV_HLA_PDAC_PANFRO413_2D_50e6_W632_classI_fxn1.9159.9159.2 2 2 1 12.25 4.33 4.53 7.92 68.7 5 0 0 93300000.0 M252m Hydrogen 245 (Q) 8 FAHSAVEm (A) 8 ETHFQVm F A H\S\A\V\E|m 62.5 5 5 1 1 0 b-ion bias m:Oxidized methionine 0.998 96.2 18.37 49.82 0.0 454.202 891.403 15.9937 -1.3 -1.4000000000000001 5.24 61769.9 nuORFdb_human ENST00000370082.1_1_20:62201547-62204491:- ENST00000370082.1_1_20:62201547-62204491:-|ENST00000479540.5_1_20:62203456-62204512:- HELZ2 5' Overlap uORF 5' Overlap uORF GN=HELZ2 5' Overlap uORF AL20210827_EV_HLA_PDAC_PANFRO413_2D_50e6_W632_classI_fxn1 Exploris AL20221201_MKP_PDAC_01.10808.10906.2 2 1 1 11.14 3.3 4.59 7.85 70.3 5 0 0 108000000.0 M252m Hydrogen 245 (Q) 8 FAHSAVEm (A) 8 FLHSQEm F A H\S\A\V|E|m 62.5 5 5 2 2 0 b-ion bias m:Oxidized methionine 0.998 97.2 20.23 9.65 0.0 454.203 891.403 15.9958 1.0 0.19999999999999996 5.24 61769.9 nuORFdb_human ENST00000370082.1_1_20:62201547-62204491:- ENST00000370082.1_1_20:62201547-62204491:-|ENST00000479540.5_1_20:62203456-62204512:- HELZ2 5' Overlap uORF 5' Overlap uORF GN=HELZ2 5' Overlap uORF AL20221201_MKP_PDAC_01 SCP E_20221201_NO30_400nL_HLAc1_4e7_PDAC_Slot2-6_1_3524.4534.1543.2 2 3 1 17.77 10.9 5.3 6.87 93.9 6 0 0 69900.0 M252m Hydrogen 245 (Q) 8 FAHSAVEm (A) 8 GIPMDVEm F A/H|S|A|V|E\m 75.0 6 5 5 4 0 dual b/y bias m:Oxidized methionine 15.673556 0.786304 454.2002 891.403 15.9898 -5.6 -3.1999999999999997 5.24 61769.9 nuORFdb_human ENST00000370082.1_1_20:62201547-62204491:- ENST00000370082.1_1_20:62201547-62204491:-|ENST00000479540.5_1_20:62203456-62204512:- HELZ2 5' Overlap uORF 5' Overlap uORF GN=HELZ2 5' Overlap uORF E_20221201_NO30_400nL_HLAc1_4e7_PDAC_Slot2-6_1_3524 Exploris_FAIMS VH20221207_MKP_FAIMS2022E_HLAc1_4e7_PDAC.14644.14738.2 2 1 1 12.17 3.26 4.11 8.91 68.3 5 0 0 30400000.0 M252m Hydrogen 245 (Q) 8 FAHSAVEm (A) 8 ETHFQVm F A H\S\A\V\E|m 62.5 5 5 1 1 0 b-ion bias m:Oxidized methionine 0.996 100.0 31.78 26.91 -50.0 454.201 891.403 15.9918 -3.4 -0.7999999999999998 5.24 61769.9 nuORFdb_human ENST00000370082.1_1_20:62201547-62204491:- ENST00000370082.1_1_20:62201547-62204491:-|ENST00000479540.5_1_20:62203456-62204512:- HELZ2 5' Overlap uORF 5' Overlap uORF GN=HELZ2 5' Overlap uORF VH20221207_MKP_FAIMS2022E_HLAc1_4e7_PDAC 20mio-rep2 E_20221201_NO30_400nL_HLAc1_2e7_directIP_titration_rep3_Slot2-4_1_3518.17131.1438.2 2 1 1 7.18 2.15 1.47 5.03 62.8 5 1 1 6240.0 Hydrogen 12 (M) 9 FARLVSNSW (I) 9 PFTKISNWS F A R L/V|S|N|S\W 55.6 5 4 4 3 0 dual b/y bias 59.170773 0.85233 540.2828 1079.563 -0.0045 -4.2 2.0999999999999996 9.75 3894.6 nuORFdb_human ENST00000532225.5_3_11:129763187-129764268:- ENST00000532225.5_3_11:129763187-129764268:-|ENST00000524746.5_1_11:129762731-129764268:-|ENST00000541786.5_2_12:118699334-118699418:- NFRKB 5' uORF 5' uORF GN=NFRKB 5' uORF E_20221201_NO30_400nL_HLAc1_2e7_directIP_titration_rep3_Slot2-4_1_3518 40mio-rep1 E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep1_Slot1-04_1_3496.17225.528.1 1 1 1 10.01 6.87 3.18 3.14 71.9 5 0 0 3230.0 Hydrogen 67 (V) 8 FEETGSPW (-) 8 GFPSDASANS F E\E|T|G|S|P W 50.0 4 5 4 4 0 dual b/y bias 59.499866 1.409324 952.4017 952.405 -0.0027 -2.8 0.40000000000000036 3.8 7704.7 nuORFdb_human ENST00000479665.5_2_3:28390190-28390412:- ENST00000479665.5_2_3:28390190-28390412:- AZI2 5' uORF 5' uORF GN=AZI2 5' uORF E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep1_Slot1-04_1_3496 40mio-rep2 E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep2_Slot2-5_1_3520.16901.544.1 1 1 1 12.22 6.63 4.56 5.59 74.9 6 0 0 5200.0 Hydrogen 67 (V) 8 FEETGSPW (-) 8 FGPSGGDTSQ F E|E|T|G|S|P\W 75.0 6 6 5 5 0 dual b/y bias 58.382008 1.399974 952.4003 952.405 -0.0047 -4.9 0.0 3.8 7704.7 nuORFdb_human ENST00000479665.5_2_3:28390190-28390412:- ENST00000479665.5_2_3:28390190-28390412:- AZI2 5' uORF 5' uORF GN=AZI2 5' uORF E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep2_Slot2-5_1_3520 40mio-rep3 E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep3_Slot2-5_1_3521.16917.546.1 1 1 1 8.75 6.51 4.12 2.25 67.2 6 0 0 4230.0 Hydrogen 67 (V) 8 FEETGSPW (-) 8 GFPSDASANS F/E|E/T\G|S|P W 75.0 6 4 5 3 0 dual b/y bias 58.438053 1.398362 952.3994 952.405 -0.0057 -6.0 -1.4000000000000004 3.8 7704.7 nuORFdb_human ENST00000479665.5_2_3:28390190-28390412:- ENST00000479665.5_2_3:28390190-28390412:- AZI2 5' uORF 5' uORF GN=AZI2 5' uORF E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep3_Slot2-5_1_3521 Exploris AL20221201_MKP_PDAC_01.63068.63068.2 2 2 1 12.94 4.21 12.94 8.74 74.6 7 1 1 47400000.0 Hydrogen 26 (V) 10 FFHDPLTLKF (I) 10 VHmYLPTKLF F F/H\D|P L/T|L/K/F 60.0 6 3 6 2 0 y-ion bias 0.946 100.0 95.22 100.0 0.0 632.8422 1264.672 0.0047 3.7 2.9000000000000004 6.74 4747.6 nuORFdb_human ENST00000367210.2_2_1:203771721-203771835:+ ENST00000367210.2_2_1:203771721-203771835:+|ENST00000367214.5_2_1:203770728-203771838:+ ZC3H11A 5' uORF 5' uORF GN=ZC3H11A 5' uORF AL20221201_MKP_PDAC_01 SCP E_20221201_NO30_400nL_HLAc1_4e7_PDAC_Slot2-6_1_3524.28128.1278.2 2 1 1 11.44 3.85 7.92 7.58 78.6 7 1 1 12100.0 Hydrogen 26 (V) 10 FFHDPLTLKF (I) 10 FFHKRDWKT F/F/H|D|P L T|L/K\F 70.0 7 4 6 3 0 unbiased 97.177452 0.952394 632.8384 1264.672 -0.0037 -3.0 -0.6000000000000001 6.74 4747.6 nuORFdb_human ENST00000367210.2_2_1:203771721-203771835:+ ENST00000367210.2_2_1:203771721-203771835:+|ENST00000367214.5_2_1:203770728-203771838:+ ZC3H11A 5' uORF 5' uORF GN=ZC3H11A 5' uORF E_20221201_NO30_400nL_HLAc1_4e7_PDAC_Slot2-6_1_3524 SCP E_20221201_NO30_400nL_HLAc1_4e7_PDAC_Slot2-6_1_3524.29450.392.1 1 1 1 13.68 7.84 5.83 5.83 86.4 7 0 0 23700.0 Hydrogen 15 (L) 9 FFLSGGSLF (S) 9 FFLSNLSF F F\L|S\G\G\S\L\F 77.8 7 7 1 1 0 b-ion bias 101.748764 1.489873 974.4973 974.498 -0.0012 -1.2 1.2 5.52 3049.5 nuORFdb_human ENST00000456324.5_1_19:36728687-36728771:+ ENST00000456324.5_1_19:36728687-36728771:+ ZNF146 3' dORF 3' dORF GN=ZNF146 3' dORF E_20221201_NO30_400nL_HLAc1_4e7_PDAC_Slot2-6_1_3524 10mio-rep1 E_20221201_NO30_400nL_HLAc1_1e7_directIP_titration_rep1_Slot2-3_1_3512.11161.1452.2 2 1 1 11.63 6.67 4.42 4.96 66.2 8 1 1 6850.0 Hydrogen 30 (A) 9 FGYSRHTPF (-) 9 PmVmTRTPF F/G/Y/S/R\H|T|P\F 100.0 9 4 6 2 0 unbiased 38.561779 0.843593 556.2644 1111.532 -0.0112 -10.1 -2.3999999999999995 8.75 4454.2 nuORFdb_human ENST00000398073.6_1_12:58216891-58217005:- ENST00000398073.6_1_12:58216891-58217005:- CTDSP2 3' dORF 3' dORF GN=CTDSP2 3' dORF E_20221201_NO30_400nL_HLAc1_1e7_directIP_titration_rep1_Slot2-3_1_3512 40mio-rep1 E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep1_Slot1-04_1_3496.11300.1394.2 2 6 1 16.32 8.73 8.11 7.59 80.3 8 1 1 208000.0 Hydrogen 30 (A) 9 FGYSRHTPF (-) 9 MYRSPATPF F/G/Y|S/R\H|T|P\F 100.0 9 5 6 3 0 dual b/y bias 39.035049 0.880169 556.2683 1111.532 -0.0032 -2.9 0.30000000000000027 8.75 4454.2 nuORFdb_human ENST00000398073.6_1_12:58216891-58217005:- ENST00000398073.6_1_12:58216891-58217005:- CTDSP2 3' dORF 3' dORF GN=CTDSP2 3' dORF E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep1_Slot1-04_1_3496 40mio-rep2 E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep2_Slot2-5_1_3520.10969.1418.2 2 4 1 9.57 4.93 4.07 4.65 63.4 6 1 1 124000.0 Hydrogen 30 (A) 9 FGYSRHTPF (-) 9 MYRSPATPF F/G/Y/S R H|T|P\F 66.7 6 3 5 2 0 unbiased 37.893356 0.865051 556.2676 1111.532 -0.0032 -2.9 2.0000000000000004 8.75 4454.2 nuORFdb_human ENST00000398073.6_1_12:58216891-58217005:- ENST00000398073.6_1_12:58216891-58217005:- CTDSP2 3' dORF 3' dORF GN=CTDSP2 3' dORF E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep2_Slot2-5_1_3520 40mio-rep3 E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep3_Slot2-5_1_3521.10993.1393.2 2 5 1 11.96 8.18 3.79 3.79 70.5 7 1 1 106000.0 Hydrogen 30 (A) 9 FGYSRHTPF (-) 9 MYRSPATPF F/G/Y|S R\H|T|P\F 77.8 7 5 5 3 0 dual b/y bias 37.978817 0.880915 556.2669 1111.532 -0.0052 -4.7 -0.10000000000000053 8.75 4454.2 nuORFdb_human ENST00000398073.6_1_12:58216891-58217005:- ENST00000398073.6_1_12:58216891-58217005:- CTDSP2 3' dORF 3' dORF GN=CTDSP2 3' dORF E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep3_Slot2-5_1_3521 40mio-rep4 E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep4_Slot2-5_1_3522.10992.1417.2 2 6 1 12.08 6.9 5.76 5.18 70.2 6 1 1 142000.0 Hydrogen 30 (A) 9 FGYSRHTPF (-) 9 MYRSPATPF F/G/Y/S R\H|T|P F 55.6 5 3 5 2 0 unbiased 37.960789 0.865941 556.2674 1111.532 -0.0052 -4.7 0.0 8.75 4454.2 nuORFdb_human ENST00000398073.6_1_12:58216891-58217005:- ENST00000398073.6_1_12:58216891-58217005:- CTDSP2 3' dORF 3' dORF GN=CTDSP2 3' dORF E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep4_Slot2-5_1_3522 5mio-rep1 E_20221201_NO30_400nL_HLAc1_5e6_directIP_titration_rep1_Slot1-37_1_3502.11364.1445.2 2 3 1 8.79 4.66 4.12 4.12 56.7 6 1 1 21700.0 Hydrogen 30 (A) 9 FGYSRHTPF (-) 9 MYRSPATPF F/G/Y/S R\H|T|P F 55.6 5 3 5 2 0 unbiased 39.256493 0.84799 556.2666 1111.532 -0.0052 -4.7 2.3 8.75 4454.2 nuORFdb_human ENST00000398073.6_1_12:58216891-58217005:- ENST00000398073.6_1_12:58216891-58217005:- CTDSP2 3' dORF 3' dORF GN=CTDSP2 3' dORF E_20221201_NO30_400nL_HLAc1_5e6_directIP_titration_rep1_Slot1-37_1_3502 40mio-rep1 E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep1_Slot1-04_1_3496.22641.1408.2 2 2 1 14.58 5.94 10.26 8.65 90.8 6 0 0 35900.0 Hydrogen 16 (S) 9 FHPPVPQWV (S) 9 qRPRGQPWV F H|P\P V|P|Q|W\V 55.6 5 6 4 4 0 dual b/y bias 78.215057 0.87129 553.7905 1106.578 -0.0035 -3.1 0.10000000000000009 6.74 10189.7 nuORFdb_human ENST00000571129.5_1_17:7144048-7144743:- ENST00000571129.5_1_17:7144048-7144743:- GABARAP 3' dORF 3' dORF GN=GABARAP 3' dORF E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep1_Slot1-04_1_3496 40mio-rep2 E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep2_Slot2-5_1_3520.22489.1414.2 2 2 1 12.24 5.25 7.56 6.99 84.8 6 0 0 20300.0 Hydrogen 16 (S) 9 FHPPVPQWV (S) 9 FGVKHYAGEV F H|P\P V|P-Q|W\V 55.6 5 5 3 3 1 dual b/y bias 77.690163 0.867435 553.7901 1106.578 -0.0055 -4.9 0.0 6.74 10189.7 nuORFdb_human ENST00000571129.5_1_17:7144048-7144743:- ENST00000571129.5_1_17:7144048-7144743:- GABARAP 3' dORF 3' dORF GN=GABARAP 3' dORF E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep2_Slot2-5_1_3520 40mio-rep3 E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep3_Slot2-5_1_3521.22533.1412.2 2 2 1 11.13 4.51 7.55 6.62 83.1 5 0 0 15500.0 Hydrogen 16 (S) 9 FHPPVPQWV (S) 9 KADFRGQWV F H/P P V|P|Q|W\V 44.4 4 4 4 3 0 dual b/y bias 77.83918 0.868911 553.7906 1106.578 -0.0035 -3.1 1.4999999999999996 6.74 10189.7 nuORFdb_human ENST00000571129.5_1_17:7144048-7144743:- ENST00000571129.5_1_17:7144048-7144743:- GABARAP 3' dORF 3' dORF GN=GABARAP 3' dORF E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep3_Slot2-5_1_3521 40mio-rep4 E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep4_Slot2-5_1_3522.22491.1422.2 2 2 1 13.77 5.39 9.05 8.38 91.7 5 0 0 15400.0 Hydrogen 16 (S) 9 FHPPVPQWV (S) 9 QFSVPFPWV F H|P P V|P\Q|W\V 44.4 4 5 3 3 0 dual b/y bias 77.679543 0.862578 553.7899 1106.578 -0.0055 -4.9 -0.20000000000000018 6.74 10189.7 nuORFdb_human ENST00000571129.5_1_17:7144048-7144743:- ENST00000571129.5_1_17:7144048-7144743:- GABARAP 3' dORF 3' dORF GN=GABARAP 3' dORF E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep4_Slot2-5_1_3522 40mio-rep3 E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep3_Slot2-5_1_3521.18931.1382.2 2 1 1 9.99 6.29 5.03 3.69 68.1 6 0 0 6550.0 Hydrogen 56 (F) 9 FIETESQLY (C) 9 ACLQFSQIY F I/E T\E|S\Q|L\Y 55.6 5 5 3 2 0 unbiased 65.395386 0.887849 565.2706 1129.541 -0.0065 -5.7 -1.1000000000000005 3.8 9811.1 smORF_v4|nuORFdb_human LINC01116_2:177494900-177497763:- LINC01116_2:177494900-177497763:-|ENST00000295549.8_1_2:177494902-177497775:-|TCONS_00004507_2:177494902-177502270:- LINC01116 lncRNA 2:177494900-177497763:- 2 LINC01116 lincRNA lncRNA GN=LINC01116 E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep3_Slot2-5_1_3521 Exploris AL20221201_MKP_PDAC_01.63718.63718.2 2 1 1 10.4 6.98 3.1 3.42 73.4 7 1 1 272000000.0 Hydrogen 3 (V) 11 FLAFLSREMAA (V) 11 FLASPYMRISA F/L|A|F/L-S R E\M/A-A 72.7 8 3 5 2 2 unbiased 1.0 88.8 96.65 89.58 0.0 628.3286 1255.65 -0.0004 -0.3 -1.1 6.0 2324.8 smORF_v4|nuORFdb_human BCLAF1_6:136610880-136610939:- BCLAF1_6:136610880-136610939:-|ENST00000640069.1_1_6:136610882-136610939:- BCLAF1 uORF 6:136610880-136610939:- 6 BCLAF1 5' uORF 5' uORF GN=BCLAF1 AL20221201_MKP_PDAC_01 SCP E_20221201_NO30_400nL_HLAc1_4e7_PDAC_Slot2-6_1_3524.6584.1423.2 2 1 1 13.81 5.42 8.7 8.39 89.2 6 1 2 11400.0 Hydrogen 72 (I) 9 FLARKPSAV (-) 9 FLIIQNAAV F/L/A/R K|P S\A\V 55.6 5 3 4 1 0 unbiased 22.750927 0.86172 494.799 988.594 -0.0031 -3.1 -0.7000000000000002 11.0 9126.5 nuORFdb_human ENST00000312037.5_1_5:149826506-149829126:- ENST00000312037.5_1_5:149826506-149829126:-|ENST00000407193.5_1_5:149826506-149829126:- RPS14 5' Overlap uORF 5' Overlap uORF GN=RPS14 5' Overlap uORF E_20221201_NO30_400nL_HLAc1_4e7_PDAC_Slot2-6_1_3524 Exploris_FAIMS VH20221207_MKP_FAIMS2022E_HLAc1_4e7_PDAC.19582.19582.2 2 1 1 14.67 7.38 7.29 7.29 84.7 5 1 2 4330000.0 Hydrogen 72 (I) 9 FLARKPSAV (-) 9 FININLRV F/L|A R K|P S/A|V 44.4 4 3 5 3 0 dual b/y bias 0.851 76.3 41.43 80.64 -70.0 494.7993 988.594 -0.0026 -2.6 0.0 11.0 9126.5 nuORFdb_human ENST00000312037.5_1_5:149826506-149829126:- ENST00000312037.5_1_5:149826506-149829126:-|ENST00000407193.5_1_5:149826506-149829126:- RPS14 5' Overlap uORF 5' Overlap uORF GN=RPS14 5' Overlap uORF VH20221207_MKP_FAIMS2022E_HLAc1_4e7_PDAC SCP E_20221201_NO30_400nL_HLAc1_4e7_PDAC_Slot2-6_1_3524.22210.1320.2 2 4 1 11.75 6.36 4.69 5.39 73.8 6 1 1 19900.0 Hydrogen 116 (S) 9 FLCSLEQRL (-) 9 LFCTVWAVGL F L|C|S|L/E/Q|R-L 77.8 7 4 6 4 1 dual b/y bias Carbamidomethylation C:Carbamidomethylation, 76.731163 0.926387 583.3047 1165.603 -0.0007 -0.6 1.7999999999999998 6.0 13473.2 nuORFdb_human ENST00000470902.5_1_9:130911720-130912803:+ ENST00000470902.5_1_9:130911720-130912803:+ LCN2 5' Overlap uORF 5' Overlap uORF GN=LCN2 5' Overlap uORF E_20221201_NO30_400nL_HLAc1_4e7_PDAC_Slot2-6_1_3524 40mio-rep1 E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep1_Slot1-04_1_3496.16245.1429.2 2 1 1 11.3 7.39 4.44 3.91 81.8 6 1 1 7880.0 Hydrogen 27 (V) 9 FLDPATPRV (R) 9 LFDPARPSL F L|D|P/A/T|P R\V 55.6 5 4 5 3 0 dual b/y bias 56.115669 0.858326 508.2816 1015.557 -0.0004 -0.4 2.8000000000000003 5.84 6962.9 nuORFdb_human ENST00000407501.6_1_18:3449895-3450350:+ ENST00000407501.6_1_18:3449895-3450350:+|ENST00000405385.7_1_18:3449655-3456373:+|ENST00000343820.9_1_18:3449712-3450399:+ TGIF1 5' uORF 5' uORF GN=TGIF1 5' uORF E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep1_Slot1-04_1_3496 40mio-rep3 E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep3_Slot2-5_1_3521.15960.1468.2 2 1 1 8.63 7.05 2.26 1.58 64.6 6 1 1 7760.0 Hydrogen 27 (V) 9 FLDPATPRV (R) 9 LFDPARPSL F/L|D|P/A/T/P R V 66.7 6 2 6 2 0 y-ion bias 55.132839 0.833467 508.279 1015.557 -0.0064 -6.3 -1.7000000000000002 5.84 6962.9 nuORFdb_human ENST00000407501.6_1_18:3449895-3450350:+ ENST00000407501.6_1_18:3449895-3450350:+|ENST00000405385.7_1_18:3449655-3456373:+|ENST00000343820.9_1_18:3449712-3450399:+ TGIF1 5' uORF 5' uORF GN=TGIF1 5' uORF E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep3_Slot2-5_1_3521 Eva-50mio-3fr AL20210827_EV_HLA_PDAC_PANFRO413_2D_50e6_W632_classI_fxn3.50708.50708.2 2 1 1 10.77 4.68 4.93 6.09 87.7 8 1 1 7140000.0 Hydrogen 86 (E) 10 FLFQDFLRQA (A) 10 LFFGALFGHIY F/L|F/Q/D F/L/R-Q-A 80.0 8 1 6 1 2 y-ion bias 0.889 100.0 91.08 100.0 0.0 642.8414 1284.674 0.0021 1.6 1.1 5.84 10788.2 nuORFdb_human ENST00000229390.7_1_12:120901892-120907330:- ENST00000229390.7_1_12:120901892-120907330:- SRSF9 Out-of-Frame Out-of-Frame GN=SRSF9 Out-of-Frame AL20210827_EV_HLA_PDAC_PANFRO413_2D_50e6_W632_classI_fxn3 Exploris AL20221201_MKP_PDAC_01.66327.66327.2 2 1 1 9.33 5.21 2.97 4.11 77.4 7 1 1 84700000.0 Hydrogen 86 (E) 10 FLFQDFLRQA (A) 10 LFFGALFGHIY F/L|F/Q/D F/L/R Q-A 60.0 6 1 6 1 1 y-ion bias 0.737 74.7 98.98 46.93 0.0 642.8407 1284.674 0.0006 0.5 -0.30000000000000004 5.84 10788.2 nuORFdb_human ENST00000229390.7_1_12:120901892-120907330:- ENST00000229390.7_1_12:120901892-120907330:- SRSF9 Out-of-Frame Out-of-Frame GN=SRSF9 Out-of-Frame AL20221201_MKP_PDAC_01 20mio-rep1 E_20221201_NO30_400nL_HLAc1_2e7_directIP_titration_rep1_Slot2-4_1_3515.28114.1316.2 2 1 1 11.25 2.64 7.12 8.61 70.1 8 1 1 2980.0 Hydrogen 86 (E) 10 FLFQDFLRQA (A) 10 EIImFVAGFLE F L|F|Q/D\F/L|R\Q-A 80.0 8 5 5 3 1 unbiased 97.08046 0.928944 642.8364 1284.674 -0.0088 -6.9 -0.7000000000000002 5.84 10788.2 nuORFdb_human ENST00000229390.7_1_12:120901892-120907330:- ENST00000229390.7_1_12:120901892-120907330:- SRSF9 Out-of-Frame Out-of-Frame GN=SRSF9 Out-of-Frame E_20221201_NO30_400nL_HLAc1_2e7_directIP_titration_rep1_Slot2-4_1_3515 SCP E_20221201_NO30_400nL_HLAc1_4e7_PDAC_Slot2-6_1_3524.28943.1293.2 2 1 1 16.48 5.34 9.57 11.13 85.5 9 1 1 14800.0 Hydrogen 86 (E) 10 FLFQDFLRQA (A) 10 LFFTMTVAARQ F/L|F|Q|D\F|L|R\Q\A 100.0 10 8 6 5 0 dual b/y bias 99.994522 0.943156 642.8396 1284.674 -0.0008 -0.6 1.7999999999999998 5.84 10788.2 nuORFdb_human ENST00000229390.7_1_12:120901892-120907330:- ENST00000229390.7_1_12:120901892-120907330:- SRSF9 Out-of-Frame Out-of-Frame GN=SRSF9 Out-of-Frame E_20221201_NO30_400nL_HLAc1_4e7_PDAC_Slot2-6_1_3524 40mio-rep1 E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep1_Slot1-04_1_3496.28217.1285.2 2 3 1 21.39 8.0 10.21 13.38 98.4 9 1 1 124000.0 Hydrogen 86 (E) 10 FLFQDFLRQA (A) 10 LFFTMTVAARQ F/L|F|Q|D\F|L|R\Q\A 100.0 10 8 6 5 0 dual b/y bias 97.48172 0.948168 642.8376 1284.674 -0.0048 -3.7 -0.5 5.84 10788.2 nuORFdb_human ENST00000229390.7_1_12:120901892-120907330:- ENST00000229390.7_1_12:120901892-120907330:- SRSF9 Out-of-Frame Out-of-Frame GN=SRSF9 Out-of-Frame E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep1_Slot1-04_1_3496 40mio-rep2 E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep2_Slot2-5_1_3520.28128.1296.2 2 2 1 20.07 6.61 9.66 13.46 97.6 9 1 1 21700.0 Hydrogen 86 (E) 10 FLFQDFLRQA (A) 10 LFFTMTVAARQ F/L|F|Q|D\F|L|R\Q\A 100.0 10 8 6 5 0 dual b/y bias 97.170456 0.941535 642.8386 1284.674 -0.0028 -2.2 2.7 5.84 10788.2 nuORFdb_human ENST00000229390.7_1_12:120901892-120907330:- ENST00000229390.7_1_12:120901892-120907330:- SRSF9 Out-of-Frame Out-of-Frame GN=SRSF9 Out-of-Frame E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep2_Slot2-5_1_3520 40mio-rep3 E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep3_Slot2-5_1_3521.28137.1311.2 2 1 1 19.07 6.89 9.36 12.18 94.4 9 1 1 4080.0 Hydrogen 86 (E) 10 FLFQDFLRQA (A) 10 LFFTMTVAARQ F/L|F|Q|D\F|L|R\Q\A 100.0 10 8 6 5 0 dual b/y bias 97.19442 0.932031 642.837 1284.674 -0.0068 -5.3 -0.7000000000000002 5.84 10788.2 nuORFdb_human ENST00000229390.7_1_12:120901892-120907330:- ENST00000229390.7_1_12:120901892-120907330:- SRSF9 Out-of-Frame Out-of-Frame GN=SRSF9 Out-of-Frame E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep3_Slot2-5_1_3521 40mio-rep4 E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep4_Slot2-5_1_3522.28141.1304.2 2 1 1 16.72 6.36 7.08 10.36 89.3 9 1 1 8180.0 Hydrogen 86 (E) 10 FLFQDFLRQA (A) 10 LFFTMTVAARQ F/L|F|Q|D\F|L/R\Q\A 100.0 10 7 6 4 0 dual b/y bias 97.198471 0.936264 642.8367 1284.674 -0.0068 -5.3 -0.5999999999999996 5.84 10788.2 nuORFdb_human ENST00000229390.7_1_12:120901892-120907330:- ENST00000229390.7_1_12:120901892-120907330:- SRSF9 Out-of-Frame Out-of-Frame GN=SRSF9 Out-of-Frame E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep4_Slot2-5_1_3522 20mio-rep2 E_20221201_NO30_400nL_HLAc1_2e7_directIP_titration_rep3_Slot2-4_1_3518.22988.513.1 1 2 1 9.08 4.94 4.9 4.14 76.3 6 0 0 3580.0 Hydrogen 38 (Y) 9 FLFSDPATA (S) 9 ASSLnFSEI F L/F/S/D|P A\T\A 55.6 5 3 4 1 0 unbiased 79.40126 1.418286 968.4682 968.472 -0.0044 -4.5 1.7999999999999998 3.8 8603.6 nuORFdb_human ENST00000257663.3_1_7:77423607-77427674:- ENST00000257663.3_1_7:77423607-77427674:- TMEM60 5' Overlap uORF 5' Overlap uORF GN=TMEM60 5' Overlap uORF E_20221201_NO30_400nL_HLAc1_2e7_directIP_titration_rep3_Slot2-4_1_3518 40mio-rep1 E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep1_Slot1-04_1_3496.23178.506.1 1 2 1 15.05 4.78 9.48 10.27 88.5 6 0 0 7060.0 Hydrogen 38 (Y) 9 FLFSDPATA (S) 9 TQPAPnAPAT F L|F|S|D|P A\T\A 55.6 5 6 4 4 0 dual b/y bias 80.070168 1.42219 968.4695 968.472 -0.0034 -3.5 -0.2999999999999998 3.8 8603.6 nuORFdb_human ENST00000257663.3_1_7:77423607-77427674:- ENST00000257663.3_1_7:77423607-77427674:- TMEM60 5' Overlap uORF 5' Overlap uORF GN=TMEM60 5' Overlap uORF E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep1_Slot1-04_1_3496 40mio-rep4 E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep4_Slot2-5_1_3522.22925.527.1 1 3 1 17.26 4.28 12.07 12.98 94.3 7 0 0 7170.0 Hydrogen 38 (Y) 9 FLFSDPATA (S) 9 TQPAPnAPAT F L|F|S|D|P\A\T\A 77.8 7 7 4 4 0 dual b/y bias 79.180809 1.40997 968.4703 968.472 -0.0024 -2.5 2.2 3.8 8603.6 nuORFdb_human ENST00000257663.3_1_7:77423607-77427674:- ENST00000257663.3_1_7:77423607-77427674:- TMEM60 5' Overlap uORF 5' Overlap uORF GN=TMEM60 5' Overlap uORF E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep4_Slot2-5_1_3522 40mio-rep1 E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep1_Slot1-04_1_3496.27834.185.1 1 1 1 14.73 6.69 5.76 8.04 89.9 8 0 0 6620.0 Hydrogen 38 (Y) 11 FLFSDPATASL (N) 11 FISFnnQLAL F L/F/S/D|P A\T\A\S\L 63.6 7 5 4 1 0 unbiased 96.158371 1.612059 1168.5831 1168.589 -0.0085 -7.2 -4.0 3.8 8603.6 nuORFdb_human ENST00000257663.3_1_7:77423607-77427674:- ENST00000257663.3_1_7:77423607-77427674:- TMEM60 5' Overlap uORF 5' Overlap uORF GN=TMEM60 5' Overlap uORF E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep1_Slot1-04_1_3496 40mio-rep2 E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep2_Slot2-5_1_3520.27699.201.1 1 3 1 17.92 9.55 8.8 8.37 95.0 9 0 0 10800.0 Hydrogen 38 (Y) 11 FLFSDPATASL (N) 11 LFFSPDKDSI F L/F|S/D|P\A\T\A\S\L 81.8 9 7 4 2 0 unbiased 95.686607 1.602606 1168.5834 1168.589 -0.0085 -7.2 -2.3 3.8 8603.6 nuORFdb_human ENST00000257663.3_1_7:77423607-77427674:- ENST00000257663.3_1_7:77423607-77427674:- TMEM60 5' Overlap uORF 5' Overlap uORF GN=TMEM60 5' Overlap uORF E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep2_Slot2-5_1_3520 40mio-rep4 E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep4_Slot2-5_1_3522.27705.1376.2 2 2 1 13.63 4.21 5.35 9.42 80.2 6 0 0 7380.0 Hydrogen 38 (Y) 11 FLFSDPATASL (N) 11 FLEVEGAGFSL F L|F-S D|P A/T\A|S\L 45.5 5 5 4 3 1 unbiased 95.692513 0.891683 584.7942 1168.589 -0.0077 -6.6 -1.8999999999999995 3.8 8603.6 nuORFdb_human ENST00000257663.3_1_7:77423607-77427674:- ENST00000257663.3_1_7:77423607-77427674:- TMEM60 5' Overlap uORF 5' Overlap uORF GN=TMEM60 5' Overlap uORF E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep4_Slot2-5_1_3522 SCP E_20221201_NO30_400nL_HLAc1_4e7_PDAC_Slot2-6_1_3524.28611.1374.2 2 2 1 20.99 13.54 13.83 7.46 94.2 8 0 0 54800.0 Hydrogen 17 (D) 9 FLIQHLPLV (-) 9 IFHLQLIPV F/L|I|Q|H|L|P\L\V 100.0 9 7 6 5 0 dual b/y bias 98.848137 0.892866 540.3336 1079.661 -0.0005 -0.4 2.0 6.74 3038.5 nuORFdb_human ENST00000430069.5_1_3:176771676-176782752:- ENST00000430069.5_1_3:176771676-176782752:-|ENST00000424913.5_1_3:176771673-176816307:- TBL1XR1 Out-of-Frame Out-of-Frame GN=TBL1XR1 Out-of-Frame E_20221201_NO30_400nL_HLAc1_4e7_PDAC_Slot2-6_1_3524 40mio-rep1 E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep1_Slot1-04_1_3496.26802.1344.2 2 2 1 17.17 11.6 11.06 5.57 83.6 8 0 0 13300.0 Hydrogen 17 (D) 9 FLIQHLPLV (-) 9 IFHLQLIPV F/L|I/Q\H|L|P\L\V 100.0 9 6 5 3 0 dual b/y bias 92.591141 0.911115 540.3319 1079.661 -0.0045 -4.1 -0.8999999999999995 6.74 3038.5 nuORFdb_human ENST00000430069.5_1_3:176771676-176782752:- ENST00000430069.5_1_3:176771676-176782752:-|ENST00000424913.5_1_3:176771673-176816307:- TBL1XR1 Out-of-Frame Out-of-Frame GN=TBL1XR1 Out-of-Frame E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep1_Slot1-04_1_3496 40mio-rep2 E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep2_Slot2-5_1_3520.26607.1360.2 2 2 1 14.96 10.74 9.02 4.23 81.4 7 0 0 8060.0 Hydrogen 17 (D) 9 FLIQHLPLV (-) 9 IFHLQLIPV F L|I/Q/H\L|P\L\V 77.8 7 5 4 2 0 unbiased 91.915459 0.901546 540.3307 1079.661 -0.0065 -6.0 -1.0999999999999996 6.74 3038.5 nuORFdb_human ENST00000430069.5_1_3:176771676-176782752:- ENST00000430069.5_1_3:176771676-176782752:-|ENST00000424913.5_1_3:176771673-176816307:- TBL1XR1 Out-of-Frame Out-of-Frame GN=TBL1XR1 Out-of-Frame E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep2_Slot2-5_1_3520 40mio-rep3 E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep3_Slot2-5_1_3521.26665.1369.2 2 2 1 12.61 7.21 9.13 5.4 79.3 8 0 0 7640.0 Hydrogen 17 (D) 9 FLIQHLPLV (-) 9 IFHLQLIPV F/L/I/Q/H\L|P\L\V 100.0 9 4 5 1 0 unbiased 92.10923 0.895723 540.3329 1079.661 -0.0025 -2.3 2.3 6.74 3038.5 nuORFdb_human ENST00000430069.5_1_3:176771676-176782752:- ENST00000430069.5_1_3:176771676-176782752:-|ENST00000424913.5_1_3:176771673-176816307:- TBL1XR1 Out-of-Frame Out-of-Frame GN=TBL1XR1 Out-of-Frame E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep3_Slot2-5_1_3521 40mio-rep4 E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep4_Slot2-5_1_3522.26665.1373.2 2 1 1 12.63 9.17 6.78 3.46 80.7 7 0 0 4780.0 Hydrogen 17 (D) 9 FLIQHLPLV (-) 9 IFHLQLIPV F L|I/Q/H\L|P\L\V 77.8 7 5 4 2 0 unbiased 92.101898 0.893423 540.3324 1079.661 -0.0045 -4.1 0.6000000000000005 6.74 3038.5 nuORFdb_human ENST00000430069.5_1_3:176771676-176782752:- ENST00000430069.5_1_3:176771676-176782752:-|ENST00000424913.5_1_3:176771673-176816307:- TBL1XR1 Out-of-Frame Out-of-Frame GN=TBL1XR1 Out-of-Frame E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep4_Slot2-5_1_3522 40mio-rep1 E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep1_Slot1-04_1_3496.21328.1368.2 2 2 1 14.39 10.09 4.72 4.3 82.9 7 1 1 14100.0 Hydrogen 8 (D) 9 FLLEIRTEA (C) 9 FLINTKDLQ F/L|L|E|I|R|T E\A 77.8 7 6 6 5 0 dual b/y bias 73.677391 0.896463 546.3077 1091.61 -0.0008 -0.7 2.5 4.53 4149.8 nuORFdb_human ENST00000382911.8_2_18:51885177-51886935:+ ENST00000382911.8_2_18:51885177-51886935:+ C18orf54 5' uORF 5' uORF GN=C18orf54 5' uORF E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep1_Slot1-04_1_3496 40mio-rep2 E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep2_Slot2-5_1_3520.21109.1386.2 2 1 1 10.27 7.65 2.66 2.62 71.1 6 1 1 6310.0 Hydrogen 8 (D) 9 FLLEIRTEA (C) 9 LFEISLQIE F/L|L|E/I|R T E\A 66.7 6 4 5 3 0 dual b/y bias 72.92366 0.885085 546.304 1091.61 -0.0088 -8.1 -3.1999999999999993 4.53 4149.8 nuORFdb_human ENST00000382911.8_2_18:51885177-51886935:+ ENST00000382911.8_2_18:51885177-51886935:+ C18orf54 5' uORF 5' uORF GN=C18orf54 5' uORF E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep2_Slot2-5_1_3520 40mio-rep1 E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep1_Slot1-04_1_3496.23690.554.1 1 4 1 9.83 5.12 3.59 4.71 88.4 8 0 0 17200.0 Hydrogen 45 (T) 9 FLPPAPAAL (G) 9 FIPFFII F/L/P/P\A|P-A\A\L 100.0 9 4 4 1 1 unbiased 81.838463 1.393778 896.5186 896.524 -0.005 -5.6 -2.3999999999999995 5.52 12033.4 nuORFdb_human ENST00000407319.6_1_22:38142252-38150984:+ ENST00000407319.6_1_22:38142252-38150984:+ TRIOBP Out-of-Frame Out-of-Frame GN=TRIOBP Out-of-Frame E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep1_Slot1-04_1_3496 40mio-rep1 E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep1_Slot1-04_1_3496.28715.256.1 1 2 1 13.7 6.0 9.02 7.7 90.0 7 0 0 10700.0 Hydrogen 10 (R) 9 FLYPNVSLL (-) 9 APAPSPAASALL F L/Y|P\N\V\S\L\L 77.8 7 6 2 1 0 b-ion bias 99.201591 1.570038 1065.5947 1065.598 -0.0079 -7.4 -4.2 5.52 2201.5 nuORFdb_human ENST00000394434.6_1_5:145551543-145552296:- ENST00000394434.6_1_5:145551543-145552296:- LARS Out-of-Frame Out-of-Frame GN=LARS Out-of-Frame E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep1_Slot1-04_1_3496 40mio-rep2 E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep2_Slot2-5_1_3520.28651.269.1 1 1 1 11.53 5.29 7.34 6.24 80.1 6 0 0 5270.0 Hydrogen 10 (R) 9 FLYPNVSLL (-) 9 APAPSPAASALL F L/Y|P N|V\S\L\L 55.6 5 5 3 2 0 unbiased 98.976021 1.562278 1065.5948 1065.598 -0.0079 -7.4 -2.5 5.52 2201.5 nuORFdb_human ENST00000394434.6_1_5:145551543-145552296:- ENST00000394434.6_1_5:145551543-145552296:- LARS Out-of-Frame Out-of-Frame GN=LARS Out-of-Frame E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep2_Slot2-5_1_3520 20mio-rep1 E_20221201_NO30_400nL_HLAc1_2e7_directIP_titration_rep1_Slot2-4_1_3515.28958.257.1 1 1 1 11.37 7.14 5.41 4.24 82.1 5 0 0 4410.0 Hydrogen 40 (N) 9 FLYSPDILI (-) 9 nIPLSYFLI F L Y|S|P D\I\L\I 44.4 4 5 2 2 0 b-ion bias 99.995018 1.569697 1080.5941 1080.598 -0.0076 -7.0 -0.7999999999999998 3.8 5193.0 smORF_v4|nuORFdb_human SNRK_3:43328078-43344646:+ SNRK_3:43328078-43344646:+|ENST00000429705.6_1_3:43328077-43344643:+|ENST00000296088.11_1_3:43341274-43344646:+ SNRK uORF 3:43328078-43344646:+ 3 SNRK 5' uORF 5' uORF GN=SNRK E_20221201_NO30_400nL_HLAc1_2e7_directIP_titration_rep1_Slot2-4_1_3515 20mio-rep2 E_20221201_NO30_400nL_HLAc1_2e7_directIP_titration_rep3_Slot2-4_1_3518.28969.250.1 1 1 1 9.16 4.57 5.71 4.59 67.9 5 0 0 4040.0 Hydrogen 40 (N) 9 FLYSPDILI (-) 9 KMNHPDLLI F L Y|S|P D\I\L\I 44.4 4 5 2 2 0 b-ion bias 100.055305 1.573529 1080.5891 1080.598 -0.0076 -7.0 -0.7000000000000002 3.8 5193.0 smORF_v4|nuORFdb_human SNRK_3:43328078-43344646:+ SNRK_3:43328078-43344646:+|ENST00000429705.6_1_3:43328077-43344643:+|ENST00000296088.11_1_3:43341274-43344646:+ SNRK uORF 3:43328078-43344646:+ 3 SNRK 5' uORF 5' uORF GN=SNRK E_20221201_NO30_400nL_HLAc1_2e7_directIP_titration_rep3_Slot2-4_1_3518 20mio-rep3 E_20221201_NO30_400nL_HLAc1_2e7_directIP_titration_rep4_Slot2-4_1_3519.28970.247.1 1 1 1 11.52 6.37 7.51 5.15 79.6 5 0 0 3230.0 Hydrogen 40 (N) 9 FLYSPDILI (-) 9 nIPLSYFLI F L Y|S|P D|I\L\I 44.4 4 5 3 3 0 dual b/y bias 100.058846 1.575323 1080.593 1080.598 -0.0076 -7.0 -1.2000000000000002 3.8 5193.0 smORF_v4|nuORFdb_human SNRK_3:43328078-43344646:+ SNRK_3:43328078-43344646:+|ENST00000429705.6_1_3:43328077-43344643:+|ENST00000296088.11_1_3:43341274-43344646:+ SNRK uORF 3:43328078-43344646:+ 3 SNRK 5' uORF 5' uORF GN=SNRK E_20221201_NO30_400nL_HLAc1_2e7_directIP_titration_rep4_Slot2-4_1_3519 SCP E_20221201_NO30_400nL_HLAc1_4e7_PDAC_Slot2-6_1_3524.29351.230.1 1 1 1 12.43 7.44 6.43 4.99 82.4 5 0 0 28500.0 Hydrogen 40 (N) 9 FLYSPDILI (-) 9 FYTVInPLL F L Y|S|P D\I\L\I 44.4 4 5 2 2 0 b-ion bias 101.406303 1.585331 1080.5954 1080.598 0.0024 2.2 4.6 3.8 5193.0 smORF_v4|nuORFdb_human SNRK_3:43328078-43344646:+ SNRK_3:43328078-43344646:+|ENST00000429705.6_1_3:43328077-43344643:+|ENST00000296088.11_1_3:43341274-43344646:+ SNRK uORF 3:43328078-43344646:+ 3 SNRK 5' uORF 5' uORF GN=SNRK E_20221201_NO30_400nL_HLAc1_4e7_PDAC_Slot2-6_1_3524 40mio-rep2 E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep2_Slot2-5_1_3520.28964.245.1 1 2 1 13.31 7.24 7.2 6.07 89.4 5 0 0 25800.0 Hydrogen 40 (N) 9 FLYSPDILI (-) 9 nIPLSYFLI F L Y|S|P D\I\L\I 44.4 4 5 2 2 0 b-ion bias 100.057205 1.576741 1080.59 1080.598 -0.0076 -7.0 -2.0999999999999996 3.8 5193.0 smORF_v4|nuORFdb_human SNRK_3:43328078-43344646:+ SNRK_3:43328078-43344646:+|ENST00000429705.6_1_3:43328077-43344643:+|ENST00000296088.11_1_3:43341274-43344646:+ SNRK uORF 3:43328078-43344646:+ 3 SNRK 5' uORF 5' uORF GN=SNRK E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep2_Slot2-5_1_3520 40mio-rep3 E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep3_Slot2-5_1_3521.28966.243.1 1 3 1 12.44 7.04 6.65 5.4 83.4 5 0 0 36900.0 Hydrogen 40 (N) 9 FLYSPDILI (-) 9 nIPLSYFLI F L Y|S|P D\I\L\I 44.4 4 5 2 2 0 b-ion bias 100.056114 1.577857 1080.5918 1080.598 -0.0076 -7.0 -2.4000000000000004 3.8 5193.0 smORF_v4|nuORFdb_human SNRK_3:43328078-43344646:+ SNRK_3:43328078-43344646:+|ENST00000429705.6_1_3:43328077-43344643:+|ENST00000296088.11_1_3:43341274-43344646:+ SNRK uORF 3:43328078-43344646:+ 3 SNRK 5' uORF 5' uORF GN=SNRK E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep3_Slot2-5_1_3521 40mio-rep4 E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep4_Slot2-5_1_3522.28970.244.1 1 1 1 12.33 6.53 6.79 5.81 86.0 5 0 0 5980.0 Hydrogen 40 (N) 9 FLYSPDILI (-) 9 nIPLSYFLI F L Y|S|P D\I\L\I 44.4 4 5 2 2 0 b-ion bias 100.061142 1.576971 1080.5946 1080.598 -0.0076 -7.0 -2.3 3.8 5193.0 smORF_v4|nuORFdb_human SNRK_3:43328078-43344646:+ SNRK_3:43328078-43344646:+|ENST00000429705.6_1_3:43328077-43344643:+|ENST00000296088.11_1_3:43341274-43344646:+ SNRK uORF 3:43328078-43344646:+ 3 SNRK 5' uORF 5' uORF GN=SNRK E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep4_Slot2-5_1_3522 40mio-rep2 E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep2_Slot2-5_1_3520.8656.1539.3 3 1 1 9.63 8.36 9.63 64.4 6 0 0 6820.0 M4m Hydrogen 3 (D) 10 FmDHGADHLY (L) 10 F/m/D H/G A D\H\L\Y 50.0 5 3 3 0 0 unbiased m:Oxidized methionine 29.90494 0.788981 407.8346 1205.504 15.986 -7.3 -2.3999999999999995 5.05 5691.4 nuORFdb_human ENST00000309594.4_1_13:21751380-21751524:+ ENST00000309594.4_1_13:21751380-21751524:+ MRPL57 3' dORF 3' dORF GN=MRPL57 3' dORF E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep2_Slot2-5_1_3520 40mio-rep3 E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep3_Slot2-5_1_3521.8664.1542.3 3 1 1 10.18 2.42 10.18 7.76 65.7 7 0 0 6550.0 M4m Hydrogen 3 (D) 10 FmDHGADHLY (L) 10 mFTSQcLKE F/m/D H G\A/D\H|L\Y 70.0 7 4 4 1 0 unbiased m:Oxidized methionine 29.931391 0.787066 407.8361 1205.504 15.989 -4.8 -0.20000000000000018 5.05 5691.4 nuORFdb_human ENST00000309594.4_1_13:21751380-21751524:+ ENST00000309594.4_1_13:21751380-21751524:+ MRPL57 3' dORF 3' dORF GN=MRPL57 3' dORF E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep3_Slot2-5_1_3521 40mio-rep4 E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep4_Slot2-5_1_3522.8656.1542.3 3 1 1 12.44 3.22 7.65 9.21 71.7 8 0 0 6310.0 M4m Hydrogen 3 (D) 10 FmDHGADHLY (L) 10 EKDCCYALY F/m/D H/G/A/D\H|L\Y 80.0 8 3 6 1 0 y-ion bias m:Oxidized methionine 29.897133 0.78693 407.8356 1205.504 15.989 -4.8 -0.09999999999999964 5.05 5691.4 nuORFdb_human ENST00000309594.4_1_13:21751380-21751524:+ ENST00000309594.4_1_13:21751380-21751524:+ MRPL57 3' dORF 3' dORF GN=MRPL57 3' dORF E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep4_Slot2-5_1_3522 Exploris_FAIMS VH20221207_MKP_FAIMS2022E_HLAc1_4e7_PDAC.30380.30380.2 2 4 1 13.54 7.8 5.28 5.74 84.6 7 1 1 46100000.0 Hydrogen 28 (R) 10 FPAGSAARVY (F) 10 FPAHPEVKN F/P|A/G/S/A/A R V/Y 70.0 7 1 7 1 0 y-ion bias 0.987 69.8 59.28 120.0 -70.0 519.7711 1038.537 -0.0019 -1.8 0.8 8.75 8603.6 nuORFdb_human ENST00000257663.3_1_7:77423607-77427674:- ENST00000257663.3_1_7:77423607-77427674:- TMEM60 5' Overlap uORF 5' Overlap uORF GN=TMEM60 5' Overlap uORF VH20221207_MKP_FAIMS2022E_HLAc1_4e7_PDAC SCP E_20221201_NO30_400nL_HLAc1_4e7_PDAC_Slot2-6_1_3524.13960.1346.2 2 1 1 8.13 5.32 2.83 2.81 60.1 6 0 0 6110.0 M89m Hydrogen 85 (S) 10 FPEHmANVEL (K) 10 RCDAPENVEL F/P E/H m A\N\V\E\L 50.0 5 4 2 0 0 b-ion bias m:Oxidized methionine 48.227398 0.910309 601.7774 1186.556 15.9906 -3.6 -1.2000000000000002 4.51 14305.1 smORF_v4|nuORFdb_human LOC101927746_7:100953823-100954197:+ LOC101927746_7:100953823-100954197:+|ENST00000419422.1_1_7:100951803-100954194:+ LOC101927746 lncRNA 7:100953823-100954197:+ 7 RP11-132A1.4 Antisense lncRNA GN=LOC101927746 E_20221201_NO30_400nL_HLAc1_4e7_PDAC_Slot2-6_1_3524 SCP E_20221201_NO30_400nL_HLAc1_4e7_PDAC_Slot2-6_1_3524.14542.1499.2 2 2 1 9.7 6.84 2.86 2.86 80.6 5 1 1 15800.0 Hydrogen 10 (P) 8 FPGRDFQY (L) 8 FADLLCQY F/P/G R D\F|Q\Y 62.5 5 3 3 1 0 unbiased 50.237244 0.81415 515.24 1029.479 -0.0061 -6.0 -3.6 5.83 2477.9 nuORFdb_human ENST00000561743.5_2_16:58033574-58036432:+ ENST00000561743.5_2_16:58033574-58036432:+|ENST00000219281.7_1_16:58035433-58036432:+|ENST00000561568.5_1_16:58035485-58036432:+ USB1 5' Overlap uORF 5' Overlap uORF GN=USB1 5' Overlap uORF E_20221201_NO30_400nL_HLAc1_4e7_PDAC_Slot2-6_1_3524 SCP E_20221201_NO30_400nL_HLAc1_4e7_PDAC_Slot2-6_1_3524.28735.581.1 1 1 1 10.04 8.33 2.66 1.71 74.0 6 0 0 8020.0 Hydrogen 39 (I) 8 FPGVPLTL (H) 8 FGPVPTLL F/P G|V|P|L\T\L 75.0 6 5 4 3 0 dual b/y bias 99.276299 1.377643 843.4956 843.497 -0.0015 -1.7 0.7 5.52 8315.5 nuORFdb_human ENST00000440088.5_1_7:77325266-77325488:- ENST00000440088.5_1_7:77325266-77325488:- APTR lincRNA lncRNA GN=APTR lincRNA E_20221201_NO30_400nL_HLAc1_4e7_PDAC_Slot2-6_1_3524 Eva-50mio-3fr AL20210827_EV_HLA_PDAC_PANFRO413_2D_50e6_W632_classI_fxn3.17882.18726.2 2 10 2 20.11 9.78 13.48 10.33 94.7 8 1 1 2770000000.0 Hydrogen 99 (S) 9 FPHRLPPSY (V) 9 FPRHLSNGVS F/P|H/R-L|P|P-S/Y 100.0 9 3 6 3 2 dual b/y bias 0.998 95.1 35.0 93.49 0.0 557.2959 1113.584 0.0005 0.4 -0.09999999999999998 8.75 14634.5 nuORFdb_human ENST00000598164.2_1_19:40172096-40174784:+ ENST00000598164.2_1_19:40172096-40174784:+ LGALS17A Pseudogene Pseudogene GN=LGALS17A Pseudogene AL20210827_EV_HLA_PDAC_PANFRO413_2D_50e6_W632_classI_fxn3 Exploris AL20221201_MKP_PDAC_01.23894.24568.2 2 8 1 18.81 9.74 12.73 9.07 90.6 8 1 1 26400000000.0 Hydrogen 99 (S) 9 FPHRLPPSY (V) 9 FPRHLSNGVS F/P|H/R-L|P|P-S/Y 100.0 9 3 6 3 2 dual b/y bias 1.0 100.0 39.92 3.65 0.0 557.2959 1113.584 0.0005 0.4 -0.4 8.75 14634.5 nuORFdb_human ENST00000598164.2_1_19:40172096-40174784:+ ENST00000598164.2_1_19:40172096-40174784:+ LGALS17A Pseudogene Pseudogene GN=LGALS17A Pseudogene AL20221201_MKP_PDAC_01 SCP E_20221201_NO30_400nL_HLAc1_4e7_PDAC_Slot2-6_1_3524.10969.1361.2 2 27 1 22.46 14.23 15.31 8.23 98.6 8 1 1 1520000.0 Hydrogen 99 (S) 9 FPHRLPPSY (V) 9 IMHRLPPYS F/P|H|R/L|P|P\S\Y 100.0 9 6 6 4 0 dual b/y bias 37.896019 0.900813 557.2937 1113.584 -0.0033 -2.9 -0.5 8.75 14634.5 nuORFdb_human ENST00000598164.2_1_19:40172096-40174784:+ ENST00000598164.2_1_19:40172096-40174784:+ LGALS17A Pseudogene Pseudogene GN=LGALS17A Pseudogene E_20221201_NO30_400nL_HLAc1_4e7_PDAC_Slot2-6_1_3524 Exploris_FAIMS VH20221207_MKP_FAIMS2022E_HLAc1_4e7_PDAC.29535.30493.2 2 14 1 18.49 9.46 18.49 9.03 87.0 8 1 1 10200000000.0 Hydrogen 99 (S) 9 FPHRLPPSY (V) 9 FPRHLSNGVS F/P|H/R-L|P|P-S/Y 100.0 9 3 6 3 2 dual b/y bias 0.995 96.9 58.3 2.9 -50.0 557.2939 1113.584 -0.0036 -3.2 -0.6000000000000001 8.75 14634.5 nuORFdb_human ENST00000598164.2_1_19:40172096-40174784:+ ENST00000598164.2_1_19:40172096-40174784:+ LGALS17A Pseudogene Pseudogene GN=LGALS17A Pseudogene VH20221207_MKP_FAIMS2022E_HLAc1_4e7_PDAC Exploris_FAIMS VH20221207_MKP_FAIMS2022E_HLAc1_4e7_PDAC.18546.18546.3 3 1 1 12.4 5.88 7.07 6.52 82.7 7 2 2 8360000.0 Hydrogen 3 (G) 9 FPSKLSRAC (S) 9 FPSTLGRCK F/P/S/K/L/S-R A/C 77.8 7 0 6 0 1 y-ion bias Carbamidomethylation C:Carbamidomethylation, 0.984 81.5 39.6 45.28 -50.0 355.854 1065.551 -0.0036 -3.4 -0.7999999999999998 11.0 6283.3 smORF_v4|nuORFdb_human BMP4_14:54423268-54420118:- BMP4_14:54423268-54420118:-|ENST00000245451.8_1_14:54420070-54423396:- BMP4 uORF 14:54423268-54420118:- 14 BMP4 5' uORF 5' uORF GN=BMP4 VH20221207_MKP_FAIMS2022E_HLAc1_4e7_PDAC SCP E_20221201_NO30_400nL_HLAc1_4e7_PDAC_Slot2-6_1_3524.29911.416.1 1 1 1 13.45 7.08 7.29 6.37 90.8 6 0 0 19000.0 Hydrogen 9 (E) 9 FPVSVPIVF (V) 9 MLSVVLVPF F P V|S|V|P\I\V\F 66.7 6 6 3 3 0 dual b/y bias 103.341209 1.47606 1004.5786 1004.582 -0.0015 -1.5 0.8999999999999999 5.52 5394.3 nuORFdb_human ENST00000319511.6_1_17:42264866-42265007:+ ENST00000319511.6_1_17:42264866-42265007:+ TMUB2 5' uORF 5' uORF GN=TMUB2 5' uORF E_20221201_NO30_400nL_HLAc1_4e7_PDAC_Slot2-6_1_3524 Eva-50mio-3fr AL20210827_EV_HLA_PDAC_PANFRO413_2D_50e6_W632_classI_fxn3.32289.32289.2 2 2 1 12.24 7.81 5.16 4.44 69.4 7 1 1 77900000.0 Hydrogen 61 (V) 9 FPWKSDSTW (H) 9 FPGnKKEmW F/P|W/K-S D-S/T|W 77.8 7 2 5 2 2 y-ion bias 0.996 89.9 59.45 100.0 0.0 577.2698 1153.531 0.0011 1.0 0.5 5.84 7961.7 nuORFdb_human ENST00000424722.5_2_2:191874614-191878846:- ENST00000424722.5_2_2:191874614-191878846:-|ENST00000392323.6_1_2:191874614-191875129:-|ENST00000409465.5_1_2:191874614-191878272:- STAT1 5' Overlap uORF 5' Overlap uORF GN=STAT1 5' Overlap uORF AL20210827_EV_HLA_PDAC_PANFRO413_2D_50e6_W632_classI_fxn3 Exploris AL20221201_MKP_PDAC_01.42353.42478.2 2 1 1 12.54 8.52 4.59 4.02 68.8 6 1 1 188000000.0 Hydrogen 61 (V) 9 FPWKSDSTW (H) 9 FPGnKKEmW F/P|W/K-S D S/T|W 66.7 6 2 5 2 1 y-ion bias 0.992 75.8 65.27 11.0 0.0 577.2686 1153.531 -0.0014 -1.2 -2.0 5.84 7961.7 nuORFdb_human ENST00000424722.5_2_2:191874614-191878846:- ENST00000424722.5_2_2:191874614-191878846:-|ENST00000392323.6_1_2:191874614-191875129:-|ENST00000409465.5_1_2:191874614-191878272:- STAT1 5' Overlap uORF 5' Overlap uORF GN=STAT1 5' Overlap uORF AL20221201_MKP_PDAC_01 SCP E_20221201_NO30_400nL_HLAc1_4e7_PDAC_Slot2-6_1_3524.19670.1412.2 2 16 1 15.47 8.66 6.34 6.81 89.9 7 1 1 202000.0 Hydrogen 61 (V) 9 FPWKSDSTW (H) 9 FPIEYPDSW F/P/W|K S/D\S|T\W 77.8 7 4 5 2 0 unbiased 67.952934 0.868945 577.2677 1153.531 -0.0026 -2.2 0.19999999999999973 5.84 7961.7 nuORFdb_human ENST00000424722.5_2_2:191874614-191878846:- ENST00000424722.5_2_2:191874614-191878846:-|ENST00000392323.6_1_2:191874614-191875129:-|ENST00000409465.5_1_2:191874614-191878272:- STAT1 5' Overlap uORF 5' Overlap uORF GN=STAT1 5' Overlap uORF E_20221201_NO30_400nL_HLAc1_4e7_PDAC_Slot2-6_1_3524 Exploris_FAIMS VH20221207_MKP_FAIMS2022E_HLAc1_4e7_PDAC.55945.56707.2 2 4 1 15.28 8.28 7.31 7.0 87.4 7 1 1 340000000.0 Hydrogen 61 (V) 9 FPWKSDSTW (H) 9 FPGnKKEmW F/P|W|K-S D-S/T|W 77.8 7 3 5 3 2 dual b/y bias 0.999 98.5 97.63 47.83 -70.0 577.2679 1153.531 -0.0027 -2.3 0.30000000000000027 5.84 7961.7 nuORFdb_human ENST00000424722.5_2_2:191874614-191878846:- ENST00000424722.5_2_2:191874614-191878846:-|ENST00000392323.6_1_2:191874614-191875129:-|ENST00000409465.5_1_2:191874614-191878272:- STAT1 5' Overlap uORF 5' Overlap uORF GN=STAT1 5' Overlap uORF VH20221207_MKP_FAIMS2022E_HLAc1_4e7_PDAC 10mio-rep3 E_20221201_NO30_400nL_HLAc1_1e7_directIP_titration_rep3_Slot2-3_1_3514.14395.1386.2 2 1 1 9.32 4.84 3.9 4.48 76.0 7 1 1 3360.0 Hydrogen 38 (V) 9 FQDPYTRSL (-) 9 FQnSLQEFI F/Q|D|P/Y/T R\S\L 77.8 7 4 5 2 0 unbiased 49.731037 0.885026 563.7762 1126.553 -0.008 -7.1 -0.7999999999999998 5.83 5268.0 nuORFdb_human ENST00000474760.1_1_6:105609928-105627738:- ENST00000474760.1_1_6:105609928-105627738:- POPDC3 ncRNA Processed Transcript lncRNA GN=POPDC3 ncRNA Processed Transcript E_20221201_NO30_400nL_HLAc1_1e7_directIP_titration_rep3_Slot2-3_1_3514 20mio-rep1 E_20221201_NO30_400nL_HLAc1_2e7_directIP_titration_rep1_Slot2-4_1_3515.14376.1382.2 2 1 1 11.22 5.45 3.13 5.77 85.0 8 1 1 5870.0 Hydrogen 38 (V) 9 FQDPYTRSL (-) 9 FQnSLQEFI F/Q|D|P/Y|T\R\S\L 100.0 9 6 5 3 0 dual b/y bias 49.652168 0.88762 563.7767 1126.553 -0.006 -5.3 0.9000000000000004 5.83 5268.0 nuORFdb_human ENST00000474760.1_1_6:105609928-105627738:- ENST00000474760.1_1_6:105609928-105627738:- POPDC3 ncRNA Processed Transcript lncRNA GN=POPDC3 ncRNA Processed Transcript E_20221201_NO30_400nL_HLAc1_2e7_directIP_titration_rep1_Slot2-4_1_3515 20mio-rep2 E_20221201_NO30_400nL_HLAc1_2e7_directIP_titration_rep3_Slot2-4_1_3518.14481.1374.2 2 1 1 10.15 6.9 2.67 3.25 83.3 7 1 1 6700.0 Hydrogen 38 (V) 9 FQDPYTRSL (-) 9 FQDITSVVAm F/Q|D|P/Y/T/R S\L 77.8 7 3 6 2 0 y-ion bias 50.019264 0.892937 563.7753 1126.553 -0.01 -8.9 -2.6000000000000005 5.83 5268.0 nuORFdb_human ENST00000474760.1_1_6:105609928-105627738:- ENST00000474760.1_1_6:105609928-105627738:- POPDC3 ncRNA Processed Transcript lncRNA GN=POPDC3 ncRNA Processed Transcript E_20221201_NO30_400nL_HLAc1_2e7_directIP_titration_rep3_Slot2-4_1_3518 20mio-rep3 E_20221201_NO30_400nL_HLAc1_2e7_directIP_titration_rep4_Slot2-4_1_3519.14408.1374.2 2 1 1 10.06 5.92 3.11 4.15 80.7 7 1 1 6080.0 Hydrogen 38 (V) 9 FQDPYTRSL (-) 9 FQnGKSKTSE F/Q/D|P/Y/T\R\S L 77.8 7 3 5 1 0 unbiased 49.766159 0.892517 563.7757 1126.553 -0.008 -7.1 -1.2999999999999998 5.83 5268.0 nuORFdb_human ENST00000474760.1_1_6:105609928-105627738:- ENST00000474760.1_1_6:105609928-105627738:- POPDC3 ncRNA Processed Transcript lncRNA GN=POPDC3 ncRNA Processed Transcript E_20221201_NO30_400nL_HLAc1_2e7_directIP_titration_rep4_Slot2-4_1_3519 40mio-rep1 E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep1_Slot1-04_1_3496.14641.1343.2 2 2 1 11.0 8.36 3.12 2.64 85.1 6 1 1 16000.0 Hydrogen 38 (V) 9 FQDPYTRSL (-) 9 FQnKnmILT F/Q|D|P/Y/T R\S L 55.6 5 3 5 2 0 unbiased 50.574337 0.911916 563.7789 1126.553 -0.002 -1.8 1.4000000000000001 5.83 5268.0 nuORFdb_human ENST00000474760.1_1_6:105609928-105627738:- ENST00000474760.1_1_6:105609928-105627738:- POPDC3 ncRNA Processed Transcript lncRNA GN=POPDC3 ncRNA Processed Transcript E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep1_Slot1-04_1_3496 40mio-rep2 E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep2_Slot2-5_1_3520.14338.1359.2 2 2 1 12.91 9.28 1.78 3.63 84.0 8 1 1 18400.0 Hydrogen 38 (V) 9 FQDPYTRSL (-) 9 FQnSLQEFI F/Q|D|P/Y/T\R\S\L 100.0 9 5 5 2 0 unbiased 49.529255 0.902013 563.7767 1126.553 -0.006 -5.3 -0.39999999999999947 5.83 5268.0 nuORFdb_human ENST00000474760.1_1_6:105609928-105627738:- ENST00000474760.1_1_6:105609928-105627738:- POPDC3 ncRNA Processed Transcript lncRNA GN=POPDC3 ncRNA Processed Transcript E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep2_Slot2-5_1_3520 40mio-rep3 E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep3_Slot2-5_1_3521.14339.1365.2 2 1 1 15.63 8.79 6.3 6.83 83.9 8 1 1 8590.0 Hydrogen 38 (V) 9 FQDPYTRSL (-) 9 FQDYVARVE F/Q|D|P|Y|T/R-S\L 100.0 9 5 6 4 1 dual b/y bias 49.5359 0.898203 563.7797 1126.553 -0.0 -0.0 4.6 5.83 5268.0 nuORFdb_human ENST00000474760.1_1_6:105609928-105627738:- ENST00000474760.1_1_6:105609928-105627738:- POPDC3 ncRNA Processed Transcript lncRNA GN=POPDC3 ncRNA Processed Transcript E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep3_Slot2-5_1_3521 40mio-rep4 E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep4_Slot2-5_1_3522.14339.1361.2 2 1 1 10.42 9.37 0.52 1.05 84.5 6 1 1 11300.0 Hydrogen 38 (V) 9 FQDPYTRSL (-) 9 FQnSLQEFI F/Q|D|P Y/T\R S\L 55.6 5 4 4 2 0 unbiased 49.521866 0.900937 563.7754 1126.553 -0.01 -8.9 -4.2 5.83 5268.0 nuORFdb_human ENST00000474760.1_1_6:105609928-105627738:- ENST00000474760.1_1_6:105609928-105627738:- POPDC3 ncRNA Processed Transcript lncRNA GN=POPDC3 ncRNA Processed Transcript E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep4_Slot2-5_1_3522 5mio-rep1 E_20221201_NO30_400nL_HLAc1_5e6_directIP_titration_rep1_Slot1-37_1_3502.14719.1379.2 2 2 1 12.31 7.54 1.54 4.77 82.9 7 1 1 4930.0 Hydrogen 38 (V) 9 FQDPYTRSL (-) 9 FQDYVARVE F/Q|D|P/Y/T\R S\L 77.8 7 4 5 2 0 unbiased 50.843018 0.889254 563.7739 1126.553 -0.012 -10.7 -3.6999999999999993 5.83 5268.0 nuORFdb_human ENST00000474760.1_1_6:105609928-105627738:- ENST00000474760.1_1_6:105609928-105627738:- POPDC3 ncRNA Processed Transcript lncRNA GN=POPDC3 ncRNA Processed Transcript E_20221201_NO30_400nL_HLAc1_5e6_directIP_titration_rep1_Slot1-37_1_3502 40mio-rep2 E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep2_Slot2-5_1_3520.8099.1574.3 3 1 1 7.0 7.0 7.0 57.4 7 3 3 10000.0 Hydrogen 3 (G) 9 FRETKVKLY (Y) 9 F/R\E|T K|V/K\L\Y 77.8 7 5 4 2 0 unbiased 27.982044 0.766765 395.2311 1183.683 -0.0049 -4.2 0.7000000000000002 9.7 5469.2 nuORFdb_human ENST00000428692.5_2_2:201248643-201253989:+ ENST00000428692.5_2_2:201248643-201253989:+ SPATS2L 5' Overlap uORF 5' Overlap uORF GN=SPATS2L 5' Overlap uORF E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep2_Slot2-5_1_3520 20mio-rep1 E_20221201_NO30_400nL_HLAc1_2e7_directIP_titration_rep1_Slot2-4_1_3515.18690.1468.2 2 2 1 9.72 4.54 4.7 5.19 71.2 6 1 1 8340.0 Hydrogen 22 (S) 9 FRIPGTQSL (Q) 9 RFISPASQL F R|I|P G\T\Q\S\L 55.6 5 6 2 2 0 b-ion bias 64.546661 0.83375 509.7855 1018.568 -0.0033 -3.2 3.0 9.75 5806.7 nuORFdb_human ENST00000403627.7_1_17:71205816-71223389:- ENST00000403627.7_1_17:71205816-71223389:- FAM104A Out-of-Frame Out-of-Frame GN=FAM104A Out-of-Frame E_20221201_NO30_400nL_HLAc1_2e7_directIP_titration_rep1_Slot2-4_1_3515 40mio-rep1 E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep1_Slot1-04_1_3496.18984.1436.2 2 2 1 13.56 8.54 5.08 5.02 83.0 6 1 1 17100.0 Hydrogen 22 (S) 9 FRIPGTQSL (Q) 9 ALCILTAGSI F R|I|P\G T|Q\S\L 55.6 5 6 3 3 0 dual b/y bias 65.580048 0.853557 509.786 1018.568 -0.0033 -3.2 0.0 9.75 5806.7 nuORFdb_human ENST00000403627.7_1_17:71205816-71223389:- ENST00000403627.7_1_17:71205816-71223389:- FAM104A Out-of-Frame Out-of-Frame GN=FAM104A Out-of-Frame E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep1_Slot1-04_1_3496 40mio-rep2 E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep2_Slot2-5_1_3520.18765.1454.2 2 2 1 12.45 6.09 5.0 6.36 77.2 6 1 1 15200.0 Hydrogen 22 (S) 9 FRIPGTQSL (Q) 9 RFISPASQL F R|I|P G\T\Q\S\L 55.6 5 6 2 2 0 b-ion bias 64.824936 0.842502 509.7858 1018.568 -0.0033 -3.2 1.7000000000000002 9.75 5806.7 nuORFdb_human ENST00000403627.7_1_17:71205816-71223389:- ENST00000403627.7_1_17:71205816-71223389:- FAM104A Out-of-Frame Out-of-Frame GN=FAM104A Out-of-Frame E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep2_Slot2-5_1_3520 40mio-rep4 E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep4_Slot2-5_1_3522.18799.1463.2 2 3 1 8.05 4.69 3.62 3.37 62.0 6 1 1 18300.0 Hydrogen 22 (S) 9 FRIPGTQSL (Q) 9 ALCILTAGSI F R/I|P G\T\Q|S\L 55.6 5 5 3 2 0 unbiased 64.922691 0.836519 509.7849 1018.568 -0.0053 -5.2 -0.5 9.75 5806.7 nuORFdb_human ENST00000403627.7_1_17:71205816-71223389:- ENST00000403627.7_1_17:71205816-71223389:- FAM104A Out-of-Frame Out-of-Frame GN=FAM104A Out-of-Frame E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep4_Slot2-5_1_3522 40mio-rep1 E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep1_Slot1-04_1_3496.14681.1330.2 2 2 1 11.14 7.0 5.21 4.14 68.7 6 0 0 21900.0 M11m Hydrogen 6 (L) 9 FSDDHmWDY (F) 9 mYGSSFDMDY F/S/D D H/m/W|D\Y 66.7 6 2 5 1 0 y-ion bias m:Oxidized methionine 50.712425 0.920378 616.2192 1215.441 15.9896 -4.3 -1.0999999999999996 3.92 4626.2 nuORFdb_human ENST00000466431.6_1_3:9391398-9391506:- ENST00000466431.6_1_3:9391398-9391506:- RP11-380O24.1 Antisense lncRNA GN=RP11-380O24.1 Antisense E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep1_Slot1-04_1_3496 40mio-rep2 E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep2_Slot2-5_1_3520.14329.1347.2 2 1 1 10.6 6.79 5.42 3.81 68.6 6 0 0 7750.0 M11m Hydrogen 6 (L) 9 FSDDHmWDY (F) 9 MYGSSFDmDY F/S/D D\H m\W|D\Y 55.6 5 4 3 1 0 unbiased m:Oxidized methionine 49.49818 0.909367 616.2179 1215.441 15.9876 -6.0 -1.0999999999999996 3.92 4626.2 nuORFdb_human ENST00000466431.6_1_3:9391398-9391506:- ENST00000466431.6_1_3:9391398-9391506:- RP11-380O24.1 Antisense lncRNA GN=RP11-380O24.1 Antisense E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep2_Slot2-5_1_3520 10mio-rep3 E_20221201_NO30_400nL_HLAc1_1e7_directIP_titration_rep3_Slot2-3_1_3514.13920.1431.2 2 1 1 14.62 6.93 7.69 7.69 79.4 7 1 1 10400.0 Hydrogen 134 (C) 9 FSDPDRLTY (C) 9 SFDnLPEKY F/S/D|P/D R|L\T\Y 77.8 7 4 5 2 0 unbiased 48.090908 0.856637 557.2597 1113.521 -0.0084 -7.5 -1.2000000000000002 4.21 15869.7 nuORFdb_human ENST00000529966.5_1_11:771186-777401:- ENST00000529966.5_1_11:771186-777401:- PDDC1 ncRNA Processed Transcript lncRNA GN=PDDC1 ncRNA Processed Transcript E_20221201_NO30_400nL_HLAc1_1e7_directIP_titration_rep3_Slot2-3_1_3514 20mio-rep1 E_20221201_NO30_400nL_HLAc1_2e7_directIP_titration_rep1_Slot2-4_1_3515.13876.1427.2 2 1 1 11.52 6.98 3.47 4.54 79.4 6 1 1 11300.0 Hydrogen 134 (C) 9 FSDPDRLTY (C) 9 SFDnLPEKY F/S/D|P/D R L|T\Y 66.7 6 3 5 2 0 unbiased 47.926155 0.859451 557.2603 1113.521 -0.0084 -7.5 -1.2999999999999998 4.21 15869.7 nuORFdb_human ENST00000529966.5_1_11:771186-777401:- ENST00000529966.5_1_11:771186-777401:- PDDC1 ncRNA Processed Transcript lncRNA GN=PDDC1 ncRNA Processed Transcript E_20221201_NO30_400nL_HLAc1_2e7_directIP_titration_rep1_Slot2-4_1_3515 20mio-rep2 E_20221201_NO30_400nL_HLAc1_2e7_directIP_titration_rep3_Slot2-4_1_3518.13984.1418.2 2 1 1 13.55 6.34 6.16 7.21 78.3 7 1 1 12600.0 Hydrogen 134 (C) 9 FSDPDRLTY (C) 9 SFDnLPEKY F/S/D|P/D R\L|T\Y 77.8 7 4 5 2 0 unbiased 48.301121 0.865022 557.2607 1113.521 -0.0064 -5.7 0.5999999999999996 4.21 15869.7 nuORFdb_human ENST00000529966.5_1_11:771186-777401:- ENST00000529966.5_1_11:771186-777401:- PDDC1 ncRNA Processed Transcript lncRNA GN=PDDC1 ncRNA Processed Transcript E_20221201_NO30_400nL_HLAc1_2e7_directIP_titration_rep3_Slot2-4_1_3518 20mio-rep3 E_20221201_NO30_400nL_HLAc1_2e7_directIP_titration_rep4_Slot2-4_1_3519.13909.1422.2 2 1 1 14.81 8.57 6.25 6.25 85.3 7 1 1 10800.0 Hydrogen 134 (C) 9 FSDPDRLTY (C) 9 SFDnLPEKY F/S|D|P/D R\L|T\Y 77.8 7 5 5 3 0 dual b/y bias 48.043842 0.862692 557.26 1113.521 -0.0084 -7.5 -1.7000000000000002 4.21 15869.7 nuORFdb_human ENST00000529966.5_1_11:771186-777401:- ENST00000529966.5_1_11:771186-777401:- PDDC1 ncRNA Processed Transcript lncRNA GN=PDDC1 ncRNA Processed Transcript E_20221201_NO30_400nL_HLAc1_2e7_directIP_titration_rep4_Slot2-4_1_3519 40mio-rep1 E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep1_Slot1-04_1_3496.14172.1394.2 2 1 1 19.13 10.04 7.55 9.08 97.2 7 1 1 22900.0 Hydrogen 134 (C) 9 FSDPDRLTY (C) 9 FSTASLHSTY F/S|D|P/D R|L|T\Y 77.8 7 5 6 4 0 dual b/y bias 48.954487 0.879919 557.2632 1113.521 -0.0024 -2.1 1.1 4.21 15869.7 nuORFdb_human ENST00000529966.5_1_11:771186-777401:- ENST00000529966.5_1_11:771186-777401:- PDDC1 ncRNA Processed Transcript lncRNA GN=PDDC1 ncRNA Processed Transcript E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep1_Slot1-04_1_3496 40mio-rep2 E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep2_Slot2-5_1_3520.13865.1404.2 2 1 1 15.79 8.29 6.05 7.5 89.5 7 1 1 23400.0 Hydrogen 134 (C) 9 FSDPDRLTY (C) 9 FSTASLHSTY F/S|D|P/D R\L|T\Y 77.8 7 5 5 3 0 dual b/y bias 47.895653 0.873883 557.2621 1113.521 -0.0044 -3.9 1.0000000000000004 4.21 15869.7 nuORFdb_human ENST00000529966.5_1_11:771186-777401:- ENST00000529966.5_1_11:771186-777401:- PDDC1 ncRNA Processed Transcript lncRNA GN=PDDC1 ncRNA Processed Transcript E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep2_Slot2-5_1_3520 40mio-rep3 E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep3_Slot2-5_1_3521.13864.1409.2 2 1 1 14.82 6.94 5.79 7.89 86.1 7 1 1 16000.0 Hydrogen 134 (C) 9 FSDPDRLTY (C) 9 FSTASLHSTY F/S/D|P/D R\L|T\Y 77.8 7 4 5 2 0 unbiased 47.895947 0.870682 557.2611 1113.521 -0.0064 -5.7 -1.1000000000000005 4.21 15869.7 nuORFdb_human ENST00000529966.5_1_11:771186-777401:- ENST00000529966.5_1_11:771186-777401:- PDDC1 ncRNA Processed Transcript lncRNA GN=PDDC1 ncRNA Processed Transcript E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep3_Slot2-5_1_3521 40mio-rep4 E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep4_Slot2-5_1_3522.13865.1406.2 2 1 1 18.19 10.34 6.26 7.85 93.3 7 1 1 27400.0 Hydrogen 134 (C) 9 FSDPDRLTY (C) 9 SFDnLPEKY F/S|D|P/D R\L|T\Y 77.8 7 5 5 3 0 dual b/y bias 47.882771 0.872655 557.2622 1113.521 -0.0044 -3.9 0.8000000000000003 4.21 15869.7 nuORFdb_human ENST00000529966.5_1_11:771186-777401:- ENST00000529966.5_1_11:771186-777401:- PDDC1 ncRNA Processed Transcript lncRNA GN=PDDC1 ncRNA Processed Transcript E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep4_Slot2-5_1_3522 10mio-rep1 E_20221201_NO30_400nL_HLAc1_1e7_directIP_titration_rep1_Slot2-3_1_3512.11550.1483.2 2 3 1 11.92 8.98 7.65 2.94 77.9 8 1 1 30600.0 Hydrogen 58 (T) 9 FSNDALKTY (A) 9 YANnAIKTY F/S/N/D/A\L|K|T\Y 100.0 9 4 6 2 0 unbiased 39.904385 0.824108 529.7572 1058.515 -0.0086 -8.1 -0.39999999999999947 5.83 9361.4 nuORFdb_human ENST00000522309.1_1_X:134185341-134186138:- ENST00000522309.1_1_X:134185341-134186138:- FAM127B 3' Overlap dORF 3' Overlap dORF GN=FAM127B 3' Overlap dORF E_20221201_NO30_400nL_HLAc1_1e7_directIP_titration_rep1_Slot2-3_1_3512 10mio-rep2 E_20221201_NO30_400nL_HLAc1_1e7_directIP_titration_rep2_Slot2-3_1_3513.11501.1465.2 2 1 1 13.26 8.46 9.76 4.81 78.4 8 1 1 5880.0 Hydrogen 58 (T) 9 FSNDALKTY (A) 9 YANnAIKTY F/S/N/D|A\L/K|T\Y 100.0 9 4 6 2 0 unbiased 39.734028 0.835416 529.7583 1058.515 -0.0066 -6.2 0.5 5.83 9361.4 nuORFdb_human ENST00000522309.1_1_X:134185341-134186138:- ENST00000522309.1_1_X:134185341-134186138:- FAM127B 3' Overlap dORF 3' Overlap dORF GN=FAM127B 3' Overlap dORF E_20221201_NO30_400nL_HLAc1_1e7_directIP_titration_rep2_Slot2-3_1_3513 10mio-rep3 E_20221201_NO30_400nL_HLAc1_1e7_directIP_titration_rep3_Slot2-3_1_3514.11472.1468.2 2 1 1 15.9 11.99 12.7 3.9 82.0 8 1 1 7640.0 Hydrogen 58 (T) 9 FSNDALKTY (A) 9 YANnAIKTY F/S|N/D|A\L|K|T\Y 100.0 9 6 6 4 0 dual b/y bias 39.634098 0.833653 529.7587 1058.515 -0.0046 -4.3 2.0 5.83 9361.4 nuORFdb_human ENST00000522309.1_1_X:134185341-134186138:- ENST00000522309.1_1_X:134185341-134186138:- FAM127B 3' Overlap dORF 3' Overlap dORF GN=FAM127B 3' Overlap dORF E_20221201_NO30_400nL_HLAc1_1e7_directIP_titration_rep3_Slot2-3_1_3514 20mio-rep1 E_20221201_NO30_400nL_HLAc1_2e7_directIP_titration_rep1_Slot2-4_1_3515.11424.1464.2 2 1 1 15.84 12.43 12.03 3.41 85.1 8 1 1 8590.0 Hydrogen 58 (T) 9 FSNDALKTY (A) 9 YANnAIKTY F/S/N|D|A\L|K|T\Y 100.0 9 6 6 4 0 dual b/y bias 39.460655 0.836192 529.7586 1058.515 -0.0046 -4.3 1.9000000000000004 5.83 9361.4 nuORFdb_human ENST00000522309.1_1_X:134185341-134186138:- ENST00000522309.1_1_X:134185341-134186138:- FAM127B 3' Overlap dORF 3' Overlap dORF GN=FAM127B 3' Overlap dORF E_20221201_NO30_400nL_HLAc1_2e7_directIP_titration_rep1_Slot2-4_1_3515 20mio-rep2 E_20221201_NO30_400nL_HLAc1_2e7_directIP_titration_rep3_Slot2-4_1_3518.11572.1454.2 2 2 1 18.52 12.98 14.43 5.54 93.4 8 1 1 16100.0 Hydrogen 58 (T) 9 FSNDALKTY (A) 9 YANnAIKTY F/S/N/D|A\L|K|T\Y 100.0 9 5 6 3 0 dual b/y bias 39.970364 0.84253 529.7571 1058.515 -0.0086 -8.1 -1.7999999999999998 5.83 9361.4 nuORFdb_human ENST00000522309.1_1_X:134185341-134186138:- ENST00000522309.1_1_X:134185341-134186138:- FAM127B 3' Overlap dORF 3' Overlap dORF GN=FAM127B 3' Overlap dORF E_20221201_NO30_400nL_HLAc1_2e7_directIP_titration_rep3_Slot2-4_1_3518 20mio-rep3 E_20221201_NO30_400nL_HLAc1_2e7_directIP_titration_rep4_Slot2-4_1_3519.11465.1451.2 2 2 1 16.78 10.07 12.38 6.7 85.9 8 1 1 32300.0 Hydrogen 58 (T) 9 FSNDALKTY (A) 9 YANnAIKTY F/S/N/D|A\L|K\T\Y 100.0 9 5 5 2 0 unbiased 39.60667 0.84406 529.7581 1058.515 -0.0066 -6.2 -0.40000000000000036 5.83 9361.4 nuORFdb_human ENST00000522309.1_1_X:134185341-134186138:- ENST00000522309.1_1_X:134185341-134186138:- FAM127B 3' Overlap dORF 3' Overlap dORF GN=FAM127B 3' Overlap dORF E_20221201_NO30_400nL_HLAc1_2e7_directIP_titration_rep4_Slot2-4_1_3519 40mio-rep1 E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep1_Slot1-04_1_3496.11751.1422.2 2 1 1 15.03 9.39 11.28 5.64 86.6 8 1 1 17700.0 Hydrogen 58 (T) 9 FSNDALKTY (A) 9 YANnAIKTY F/S/N/D|A\L|K/T\Y 100.0 9 4 6 2 0 unbiased 40.592548 0.862774 529.7598 1058.515 -0.0026 -2.4 0.8000000000000003 5.83 9361.4 nuORFdb_human ENST00000522309.1_1_X:134185341-134186138:- ENST00000522309.1_1_X:134185341-134186138:- FAM127B 3' Overlap dORF 3' Overlap dORF GN=FAM127B 3' Overlap dORF E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep1_Slot1-04_1_3496 40mio-rep2 E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep2_Slot2-5_1_3520.11431.1437.2 2 1 1 18.61 12.51 13.3 6.1 91.6 8 1 1 31300.0 Hydrogen 58 (T) 9 FSNDALKTY (A) 9 YANnAIKTY F/S|N/D|A\L|K|T\Y 100.0 9 6 6 4 0 dual b/y bias 39.488041 0.853431 529.7599 1058.515 -0.0026 -2.4 2.5000000000000004 5.83 9361.4 nuORFdb_human ENST00000522309.1_1_X:134185341-134186138:- ENST00000522309.1_1_X:134185341-134186138:- FAM127B 3' Overlap dORF 3' Overlap dORF GN=FAM127B 3' Overlap dORF E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep2_Slot2-5_1_3520 40mio-rep3 E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep3_Slot2-5_1_3521.11423.1450.2 2 2 1 19.05 14.59 13.03 4.46 93.5 8 1 1 68100.0 Hydrogen 58 (T) 9 FSNDALKTY (A) 9 YANnAIKTY F/S/N|D|A\L|K|T\Y 100.0 9 6 6 4 0 dual b/y bias 39.463585 0.844699 529.7594 1058.515 -0.0046 -4.3 0.2999999999999998 5.83 9361.4 nuORFdb_human ENST00000522309.1_1_X:134185341-134186138:- ENST00000522309.1_1_X:134185341-134186138:- FAM127B 3' Overlap dORF 3' Overlap dORF GN=FAM127B 3' Overlap dORF E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep3_Slot2-5_1_3521 40mio-rep4 E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep4_Slot2-5_1_3522.11455.1436.2 2 2 1 16.58 12.06 12.61 4.51 90.5 8 1 1 70700.0 Hydrogen 58 (T) 9 FSNDALKTY (A) 9 YANnAIKTY F/S|N/D|A\L|K|T\Y 100.0 9 6 6 4 0 dual b/y bias 39.559143 0.853927 529.759 1058.515 -0.0046 -4.3 0.40000000000000036 5.83 9361.4 nuORFdb_human ENST00000522309.1_1_X:134185341-134186138:- ENST00000522309.1_1_X:134185341-134186138:- FAM127B 3' Overlap dORF 3' Overlap dORF GN=FAM127B 3' Overlap dORF E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep4_Slot2-5_1_3522 5mio-rep1 E_20221201_NO30_400nL_HLAc1_5e6_directIP_titration_rep1_Slot1-37_1_3502.11798.1455.2 2 2 1 14.16 10.61 12.74 3.55 82.8 8 1 1 12900.0 Hydrogen 58 (T) 9 FSNDALKTY (A) 9 YANnAIKTY F/S/N/D|A\L|K|T\Y 100.0 9 5 6 3 0 dual b/y bias 40.755436 0.841992 529.7568 1058.515 -0.0086 -8.1 -1.0999999999999996 5.83 9361.4 nuORFdb_human ENST00000522309.1_1_X:134185341-134186138:- ENST00000522309.1_1_X:134185341-134186138:- FAM127B 3' Overlap dORF 3' Overlap dORF GN=FAM127B 3' Overlap dORF E_20221201_NO30_400nL_HLAc1_5e6_directIP_titration_rep1_Slot1-37_1_3502 Eva-50mio-3fr AL20210827_EV_HLA_PDAC_PANFRO413_2D_50e6_W632_classI_fxn3.17802.17802.2 2 2 2 13.11 8.02 13.11 71.9 7 0 0 4640000.0 Hydrogen 102 (V) 9 FSPHGSPHF (-) 9 F S/P/H/G\S|P/H/F 77.8 7 2 6 1 0 y-ion bias 0.987 78.2 33.8 100.0 0.0 506.7355 1012.463 0.0002 0.2 -0.3 6.92 11457.9 nuORFdb_human ENST00000376851.7_1_19:51471875-51472205:- ENST00000376851.7_1_19:51471875-51472205:- KLK6 5' uORF 5' uORF GN=KLK6 5' uORF AL20210827_EV_HLA_PDAC_PANFRO413_2D_50e6_W632_classI_fxn3 SCP E_20221201_NO30_400nL_HLAc1_4e7_PDAC_Slot2-6_1_3524.10377.1410.2 2 3 1 15.54 8.48 7.71 7.06 87.0 8 0 0 15300.0 Hydrogen 102 (V) 9 FSPHGSPHF (-) 9 WGPGAGSHPF F/S/P/H|G\S|P/H\F 100.0 9 4 6 2 0 unbiased 35.851231 0.870161 506.7345 1012.463 -0.0008 -0.8 1.5999999999999999 6.92 11457.9 nuORFdb_human ENST00000376851.7_1_19:51471875-51472205:- ENST00000376851.7_1_19:51471875-51472205:- KLK6 5' uORF 5' uORF GN=KLK6 5' uORF E_20221201_NO30_400nL_HLAc1_4e7_PDAC_Slot2-6_1_3524 40mio-rep1 E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep1_Slot1-04_1_3496.15201.1285.2 2 1 1 14.59 3.83 7.48 10.76 76.0 8 1 1 5620.0 Hydrogen 28 (C) 10 FSQEQDSRLW (S) 10 FGGTQSLHTNSF F/S/Q|E|Q|D|S R\L\W 80.0 8 6 6 4 0 dual b/y bias 52.508358 0.948304 648.3044 1295.601 -0.0008 -0.6 2.6 4.37 5081.5 nuORFdb_human ENST00000557612.5_1_14:70795945-70826359:- ENST00000557612.5_1_14:70795945-70826359:-|ENST00000389912.6_1_14:70809414-70826359:- COX16 3' Overlap dORF 3' Overlap dORF GN=COX16 3' Overlap dORF E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep1_Slot1-04_1_3496 40mio-rep3 E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep3_Slot2-5_1_3521.14881.1312.2 2 1 1 9.93 7.02 9.93 66.6 7 1 1 5490.0 Hydrogen 28 (C) 10 FSQEQDSRLW (S) 10 F S/Q|E/Q|D|S R/L\W 60.0 6 4 6 3 0 unbiased 51.4072 0.93146 648.3029 1295.601 -0.0028 -2.1 2.4999999999999996 4.37 5081.5 nuORFdb_human ENST00000557612.5_1_14:70795945-70826359:- ENST00000557612.5_1_14:70795945-70826359:-|ENST00000389912.6_1_14:70809414-70826359:- COX16 3' Overlap dORF 3' Overlap dORF GN=COX16 3' Overlap dORF E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep3_Slot2-5_1_3521 40mio-rep4 E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep4_Slot2-5_1_3522.14879.1307.2 2 1 1 7.69 -0.03 5.27 7.72 63.5 7 1 1 5410.0 Hydrogen 28 (C) 10 FSQEQDSRLW (S) 10 GEMTIQTTQASE F S/Q/E|Q/D/S R|L\W 60.0 6 3 6 2 0 y-ion bias 51.385876 0.934361 648.3016 1295.601 -0.0048 -3.7 1.0 4.37 5081.5 nuORFdb_human ENST00000557612.5_1_14:70795945-70826359:- ENST00000557612.5_1_14:70795945-70826359:-|ENST00000389912.6_1_14:70809414-70826359:- COX16 3' Overlap dORF 3' Overlap dORF GN=COX16 3' Overlap dORF E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep4_Slot2-5_1_3522 1mio-rep1 E_20221201_NO30_400nL_HLAc1_1e6_directIP_titration_rep1_Slot1-06_1_3498.11880.1430.2 2 1 1 15.16 8.72 8.62 6.44 88.7 8 1 1 2240.0 Hydrogen 28 (G) 9 FTDGITRTY (A) 9 TFnAISRTY F/T|D|G|I/T/R\T\Y 100.0 9 5 6 3 0 dual b/y bias 41.037792 0.857314 537.2635 1073.526 -0.0075 -7.0 -2.0999999999999996 5.83 4453.1 nuORFdb_human ENST00000294309.7_2_11:68821527-68822650:+ ENST00000294309.7_2_11:68821527-68822650:+ TPCN2 Out-of-Frame Out-of-Frame GN=TPCN2 Out-of-Frame E_20221201_NO30_400nL_HLAc1_1e6_directIP_titration_rep1_Slot1-06_1_3498 10mio-rep1 E_20221201_NO30_400nL_HLAc1_1e7_directIP_titration_rep1_Slot2-3_1_3512.11541.1431.2 2 1 1 16.48 8.42 11.11 8.06 87.6 8 1 1 15800.0 Hydrogen 28 (G) 9 FTDGITRTY (A) 9 TFnAISRTY F/T/D|G|I/T|R|T\Y 100.0 9 5 7 4 0 dual b/y bias 39.873306 0.856948 537.2625 1073.526 -0.0075 -7.0 0.7000000000000002 5.83 4453.1 nuORFdb_human ENST00000294309.7_2_11:68821527-68822650:+ ENST00000294309.7_2_11:68821527-68822650:+ TPCN2 Out-of-Frame Out-of-Frame GN=TPCN2 Out-of-Frame E_20221201_NO30_400nL_HLAc1_1e7_directIP_titration_rep1_Slot2-3_1_3512 10mio-rep2 E_20221201_NO30_400nL_HLAc1_1e7_directIP_titration_rep2_Slot2-3_1_3513.11499.1430.2 2 1 1 15.61 9.91 8.23 5.7 89.9 8 1 1 11300.0 Hydrogen 28 (G) 9 FTDGITRTY (A) 9 TFnGATVTVY F/T|D|G|I/T-R\T\Y 100.0 9 5 5 3 1 dual b/y bias 39.727119 0.857403 537.2633 1073.526 -0.0075 -7.0 -0.2999999999999998 5.83 4453.1 nuORFdb_human ENST00000294309.7_2_11:68821527-68822650:+ ENST00000294309.7_2_11:68821527-68822650:+ TPCN2 Out-of-Frame Out-of-Frame GN=TPCN2 Out-of-Frame E_20221201_NO30_400nL_HLAc1_1e7_directIP_titration_rep2_Slot2-3_1_3513 10mio-rep3 E_20221201_NO30_400nL_HLAc1_1e7_directIP_titration_rep3_Slot2-3_1_3514.11465.1436.2 2 1 1 16.17 9.7 8.57 6.48 89.9 8 1 1 10600.0 Hydrogen 28 (G) 9 FTDGITRTY (A) 9 TFnGATVTVY F/T|D|G|I/T/R|T\Y 100.0 9 5 7 4 0 dual b/y bias 39.60994 0.853538 537.263 1073.526 -0.0075 -7.0 -0.7000000000000002 5.83 4453.1 nuORFdb_human ENST00000294309.7_2_11:68821527-68822650:+ ENST00000294309.7_2_11:68821527-68822650:+ TPCN2 Out-of-Frame Out-of-Frame GN=TPCN2 Out-of-Frame E_20221201_NO30_400nL_HLAc1_1e7_directIP_titration_rep3_Slot2-3_1_3514 20mio-rep1 E_20221201_NO30_400nL_HLAc1_2e7_directIP_titration_rep1_Slot2-4_1_3515.11442.1425.2 2 3 1 19.31 11.34 10.95 7.97 95.3 8 1 1 81600.0 Hydrogen 28 (G) 9 FTDGITRTY (A) 9 TFnAISRTY F/T|D|G|I/T|R|T\Y 100.0 9 6 7 5 0 dual b/y bias 39.522682 0.860759 537.2629 1073.526 -0.0075 -7.0 -0.7999999999999998 5.83 4453.1 nuORFdb_human ENST00000294309.7_2_11:68821527-68822650:+ ENST00000294309.7_2_11:68821527-68822650:+ TPCN2 Out-of-Frame Out-of-Frame GN=TPCN2 Out-of-Frame E_20221201_NO30_400nL_HLAc1_2e7_directIP_titration_rep1_Slot2-4_1_3515 20mio-rep2 E_20221201_NO30_400nL_HLAc1_2e7_directIP_titration_rep3_Slot2-4_1_3518.11560.1426.2 2 2 1 19.9 12.7 11.79 7.2 97.1 8 1 1 55800.0 Hydrogen 28 (G) 9 FTDGITRTY (A) 9 TFnAISRTY F/T|D|G|I/T|R|T\Y 100.0 9 6 7 5 0 dual b/y bias 39.928947 0.859799 537.2644 1073.526 -0.0055 -5.1 1.2000000000000002 5.83 4453.1 nuORFdb_human ENST00000294309.7_2_11:68821527-68822650:+ ENST00000294309.7_2_11:68821527-68822650:+ TPCN2 Out-of-Frame Out-of-Frame GN=TPCN2 Out-of-Frame E_20221201_NO30_400nL_HLAc1_2e7_directIP_titration_rep3_Slot2-4_1_3518 20mio-rep3 E_20221201_NO30_400nL_HLAc1_2e7_directIP_titration_rep4_Slot2-4_1_3519.11455.1437.2 2 3 1 18.31 11.21 10.73 7.11 94.5 8 1 1 33600.0 Hydrogen 28 (G) 9 FTDGITRTY (A) 9 TFnAISRTY F/T|D|G|I/T|R|T\Y 100.0 9 6 7 5 0 dual b/y bias 39.572159 0.853007 537.2641 1073.526 -0.0055 -5.1 0.7000000000000002 5.83 4453.1 nuORFdb_human ENST00000294309.7_2_11:68821527-68822650:+ ENST00000294309.7_2_11:68821527-68822650:+ TPCN2 Out-of-Frame Out-of-Frame GN=TPCN2 Out-of-Frame E_20221201_NO30_400nL_HLAc1_2e7_directIP_titration_rep4_Slot2-4_1_3519 40mio-rep1 E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep1_Slot1-04_1_3496.11761.1381.2 2 2 1 21.93 13.16 12.9 8.77 97.9 8 1 1 168000.0 Hydrogen 28 (G) 9 FTDGITRTY (A) 9 TFnAISRTY F/T|D|G|I/T|R|T\Y 100.0 9 6 7 5 0 dual b/y bias 40.627083 0.888396 537.2658 1073.526 -0.0015 -1.4 1.8000000000000003 5.83 4453.1 nuORFdb_human ENST00000294309.7_2_11:68821527-68822650:+ ENST00000294309.7_2_11:68821527-68822650:+ TPCN2 Out-of-Frame Out-of-Frame GN=TPCN2 Out-of-Frame E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep1_Slot1-04_1_3496 40mio-rep2 E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep2_Slot2-5_1_3520.11443.1408.2 2 1 1 21.38 13.07 12.67 8.31 96.6 8 1 1 118000.0 Hydrogen 28 (G) 9 FTDGITRTY (A) 9 TFnAISRTY F/T|D|G|I/T/R|T\Y 100.0 9 5 7 4 0 dual b/y bias 39.529442 0.871454 537.265 1073.526 -0.0035 -3.2 1.7000000000000002 5.83 4453.1 nuORFdb_human ENST00000294309.7_2_11:68821527-68822650:+ ENST00000294309.7_2_11:68821527-68822650:+ TPCN2 Out-of-Frame Out-of-Frame GN=TPCN2 Out-of-Frame E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep2_Slot2-5_1_3520 40mio-rep3 E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep3_Slot2-5_1_3521.11443.1412.2 2 1 1 20.88 13.13 12.87 7.75 94.1 8 1 1 102000.0 Hydrogen 28 (G) 9 FTDGITRTY (A) 9 TFnAISRTY F/T|D|G|I/T|R|T\Y 100.0 9 6 7 5 0 dual b/y bias 39.532619 0.868791 537.265 1073.526 -0.0035 -3.2 1.3999999999999995 5.83 4453.1 nuORFdb_human ENST00000294309.7_2_11:68821527-68822650:+ ENST00000294309.7_2_11:68821527-68822650:+ TPCN2 Out-of-Frame Out-of-Frame GN=TPCN2 Out-of-Frame E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep3_Slot2-5_1_3521 40mio-rep4 E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep4_Slot2-5_1_3522.11454.1409.2 2 1 1 20.9 12.99 12.44 7.91 97.3 8 1 1 104000.0 Hydrogen 28 (G) 9 FTDGITRTY (A) 9 TFnAISRTY F/T|D|G|I/T|R|T\Y 100.0 9 6 7 5 0 dual b/y bias 39.555691 0.870447 537.2654 1073.526 -0.0035 -3.2 1.5 5.83 4453.1 nuORFdb_human ENST00000294309.7_2_11:68821527-68822650:+ ENST00000294309.7_2_11:68821527-68822650:+ TPCN2 Out-of-Frame Out-of-Frame GN=TPCN2 Out-of-Frame E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep4_Slot2-5_1_3522 5mio-rep1 E_20221201_NO30_400nL_HLAc1_5e6_directIP_titration_rep1_Slot1-37_1_3502.11796.1410.2 2 3 1 15.43 8.5 8.67 6.93 86.0 7 1 1 30500.0 Hydrogen 28 (G) 9 FTDGITRTY (A) 9 TFnAISRTY F/T|D|G|I/T|R\T Y 77.8 7 5 6 4 0 dual b/y bias 40.748531 0.870206 537.2627 1073.526 -0.0075 -7.0 0.0 5.83 4453.1 nuORFdb_human ENST00000294309.7_2_11:68821527-68822650:+ ENST00000294309.7_2_11:68821527-68822650:+ TPCN2 Out-of-Frame Out-of-Frame GN=TPCN2 Out-of-Frame E_20221201_NO30_400nL_HLAc1_5e6_directIP_titration_rep1_Slot1-37_1_3502 5mio-rep2 E_20221201_NO30_400nL_HLAc1_5e6_directIP_titration_rep2_Slot2-2_1_3510.11715.1437.2 2 2 1 13.1 7.51 7.31 5.59 88.6 7 1 1 8280.0 Hydrogen 28 (G) 9 FTDGITRTY (A) 9 TFnGATVTVY F/T|D|G|I/T|R T\Y 77.8 7 5 6 4 0 dual b/y bias 40.464058 0.8534 537.2611 1073.526 -0.0115 -10.7 -2.0 5.83 4453.1 nuORFdb_human ENST00000294309.7_2_11:68821527-68822650:+ ENST00000294309.7_2_11:68821527-68822650:+ TPCN2 Out-of-Frame Out-of-Frame GN=TPCN2 Out-of-Frame E_20221201_NO30_400nL_HLAc1_5e6_directIP_titration_rep2_Slot2-2_1_3510 5mio-rep3 E_20221201_NO30_400nL_HLAc1_5e6_directIP_titration_rep3_Slot2-2_1_3511.11730.1434.2 2 2 1 17.02 9.07 9.88 7.95 88.3 8 1 1 11500.0 Hydrogen 28 (G) 9 FTDGITRTY (A) 9 TFnGATVTVY F/T|D|G|I/T/R|T\Y 100.0 9 5 7 4 0 dual b/y bias 40.523636 0.855151 537.2636 1073.526 -0.0055 -5.1 3.0999999999999996 5.83 4453.1 nuORFdb_human ENST00000294309.7_2_11:68821527-68822650:+ ENST00000294309.7_2_11:68821527-68822650:+ TPCN2 Out-of-Frame Out-of-Frame GN=TPCN2 Out-of-Frame E_20221201_NO30_400nL_HLAc1_5e6_directIP_titration_rep3_Slot2-2_1_3511 40mio-rep1 E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep1_Slot1-04_1_3496.8222.1640.3 3 1 1 7.99 5.47 4.37 2.53 68.5 6 2 2 11400.0 Hydrogen 10 (T) 9 FTFKHTRQF (-) 9 FTEKSHLNVH F/T/F/K/H|T R\Q F 55.6 5 2 5 1 0 y-ion bias 28.407679 0.724823 404.5465 1211.632 -0.0056 -4.6 -1.3999999999999995 11.0 2168.5 nuORFdb_human ENST00000358738.3_1_14:64988292-64988349:+ ENST00000358738.3_1_14:64988292-64988349:+ ZBTB1 Out-of-Frame Out-of-Frame GN=ZBTB1 Out-of-Frame E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep1_Slot1-04_1_3496 10mio-rep1 E_20221201_NO30_400nL_HLAc1_1e7_directIP_titration_rep1_Slot2-3_1_3512.12960.1420.2 2 1 1 13.62 5.9 8.54 7.72 74.9 8 1 1 18200.0 Hydrogen 30 (S) 9 FTQKINSTW (F) 9 TFnEAKPIGF F/T|Q/K/I|N\S|T\W 100.0 9 5 6 3 0 dual b/y bias 44.774902 0.863546 562.7858 1124.573 -0.0088 -7.8 -0.09999999999999964 8.75 8763.2 nuORFdb_human ENST00000555408.5_1_12:56361645-56362592:+ ENST00000555408.5_1_12:56361645-56362592:+ CDK2 3' dORF 3' dORF GN=CDK2 3' dORF E_20221201_NO30_400nL_HLAc1_1e7_directIP_titration_rep1_Slot2-3_1_3512 10mio-rep2 E_20221201_NO30_400nL_HLAc1_1e7_directIP_titration_rep2_Slot2-3_1_3513.12916.1425.2 2 2 1 12.26 4.2 8.27 8.06 72.6 8 1 1 25500.0 Hydrogen 30 (S) 9 FTQKINSTW (F) 9 TAATAPPAAPAGE F/T/Q/K|I|N|S|T\W 100.0 9 5 7 4 0 dual b/y bias 44.621445 0.860667 562.787 1124.573 -0.0068 -6.0 0.7000000000000002 8.75 8763.2 nuORFdb_human ENST00000555408.5_1_12:56361645-56362592:+ ENST00000555408.5_1_12:56361645-56362592:+ CDK2 3' dORF 3' dORF GN=CDK2 3' dORF E_20221201_NO30_400nL_HLAc1_1e7_directIP_titration_rep2_Slot2-3_1_3513 10mio-rep3 E_20221201_NO30_400nL_HLAc1_1e7_directIP_titration_rep3_Slot2-3_1_3514.12879.1438.2 2 2 1 10.89 0.93 7.62 9.96 67.4 8 1 1 16400.0 Hydrogen 30 (S) 9 FTQKINSTW (F) 9 TAATAPPAAPAGE F/T/Q/K\I|N\S|T\W 100.0 9 5 5 2 0 unbiased 44.49625 0.852824 562.7877 1124.573 -0.0048 -4.2 2.0999999999999996 8.75 8763.2 nuORFdb_human ENST00000555408.5_1_12:56361645-56362592:+ ENST00000555408.5_1_12:56361645-56362592:+ CDK2 3' dORF 3' dORF GN=CDK2 3' dORF E_20221201_NO30_400nL_HLAc1_1e7_directIP_titration_rep3_Slot2-3_1_3514 20mio-rep1 E_20221201_NO30_400nL_HLAc1_2e7_directIP_titration_rep1_Slot2-4_1_3515.12851.1403.2 2 4 1 16.2 4.83 10.2 11.37 81.7 8 1 1 71300.0 Hydrogen 30 (S) 9 FTQKINSTW (F) 9 FGTSLQQKDT F/T/Q|K|I\N|S|T\W 100.0 9 6 6 4 0 dual b/y bias 44.387123 0.874484 562.7878 1124.573 -0.0048 -4.2 2.0 8.75 8763.2 nuORFdb_human ENST00000555408.5_1_12:56361645-56362592:+ ENST00000555408.5_1_12:56361645-56362592:+ CDK2 3' dORF 3' dORF GN=CDK2 3' dORF E_20221201_NO30_400nL_HLAc1_2e7_directIP_titration_rep1_Slot2-4_1_3515 20mio-rep2 E_20221201_NO30_400nL_HLAc1_2e7_directIP_titration_rep3_Slot2-4_1_3518.12960.1411.2 2 2 1 12.04 5.27 6.36 6.77 73.5 8 1 1 42800.0 Hydrogen 30 (S) 9 FTQKINSTW (F) 9 DVKKFSNTGE F/T/Q/K\I\N\S|T\W 100.0 9 5 4 1 0 unbiased 44.764599 0.869589 562.787 1124.573 -0.0068 -6.0 0.2999999999999998 8.75 8763.2 nuORFdb_human ENST00000555408.5_1_12:56361645-56362592:+ ENST00000555408.5_1_12:56361645-56362592:+ CDK2 3' dORF 3' dORF GN=CDK2 3' dORF E_20221201_NO30_400nL_HLAc1_2e7_directIP_titration_rep3_Slot2-4_1_3518 20mio-rep3 E_20221201_NO30_400nL_HLAc1_2e7_directIP_titration_rep4_Slot2-4_1_3519.12894.1415.2 2 3 1 16.85 7.45 7.85 9.4 85.7 8 1 1 70500.0 Hydrogen 30 (S) 9 FTQKINSTW (F) 9 FGTSLQQKDT F/T/Q|K\I|N|S|T\W 100.0 9 6 6 4 0 dual b/y bias 44.540031 0.867132 562.7884 1124.573 -0.0048 -4.2 1.5999999999999996 8.75 8763.2 nuORFdb_human ENST00000555408.5_1_12:56361645-56362592:+ ENST00000555408.5_1_12:56361645-56362592:+ CDK2 3' dORF 3' dORF GN=CDK2 3' dORF E_20221201_NO30_400nL_HLAc1_2e7_directIP_titration_rep4_Slot2-4_1_3519 40mio-rep1 E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep1_Slot1-04_1_3496.13137.1393.2 2 6 1 17.26 6.04 9.36 11.22 83.3 8 1 1 146000.0 Hydrogen 30 (S) 9 FTQKINSTW (F) 9 FTNNIPKSGF F/T|Q|K\I|N|S|T\W 100.0 9 7 6 5 0 dual b/y bias 45.379807 0.881052 562.7887 1124.573 -0.0028 -2.5 0.7000000000000002 8.75 8763.2 nuORFdb_human ENST00000555408.5_1_12:56361645-56362592:+ ENST00000555408.5_1_12:56361645-56362592:+ CDK2 3' dORF 3' dORF GN=CDK2 3' dORF E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep1_Slot1-04_1_3496 40mio-rep2 E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep2_Slot2-5_1_3520.12851.1405.2 2 2 1 15.72 8.37 7.34 7.34 84.9 8 1 1 98700.0 Hydrogen 30 (S) 9 FTQKINSTW (F) 9 TFnEAKPIGF F/T|Q/K\I\N|S|T\W 100.0 9 6 5 3 0 dual b/y bias 44.393806 0.873362 562.7876 1124.573 -0.0048 -4.2 0.7000000000000002 8.75 8763.2 nuORFdb_human ENST00000555408.5_1_12:56361645-56362592:+ ENST00000555408.5_1_12:56361645-56362592:+ CDK2 3' dORF 3' dORF GN=CDK2 3' dORF E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep2_Slot2-5_1_3520 40mio-rep3 E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep3_Slot2-5_1_3521.12872.1421.2 2 4 1 17.21 8.21 8.99 9.0 87.6 8 1 1 210000.0 Hydrogen 30 (S) 9 FTQKINSTW (F) 9 FTNNIPKSGF F/T|Q/K\I|N|S|T\W 100.0 9 6 6 4 0 dual b/y bias 44.470657 0.863112 562.7891 1124.573 -0.0028 -2.5 2.0999999999999996 8.75 8763.2 nuORFdb_human ENST00000555408.5_1_12:56361645-56362592:+ ENST00000555408.5_1_12:56361645-56362592:+ CDK2 3' dORF 3' dORF GN=CDK2 3' dORF E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep3_Slot2-5_1_3521 40mio-rep4 E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep4_Slot2-5_1_3522.12850.1410.2 2 6 1 20.24 6.98 10.89 13.26 92.1 8 1 1 394000.0 Hydrogen 30 (S) 9 FTQKINSTW (F) 9 TFnEAKPIGF F/T|Q|K\I|N|S|T\W 100.0 9 7 6 5 0 dual b/y bias 44.375149 0.870207 562.7884 1124.573 -0.0048 -4.2 0.5 8.75 8763.2 nuORFdb_human ENST00000555408.5_1_12:56361645-56362592:+ ENST00000555408.5_1_12:56361645-56362592:+ CDK2 3' dORF 3' dORF GN=CDK2 3' dORF E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep4_Slot2-5_1_3522 5mio-rep1 E_20221201_NO30_400nL_HLAc1_5e6_directIP_titration_rep1_Slot1-37_1_3502.13203.1411.2 2 1 1 13.7 2.97 9.19 10.73 76.0 8 1 1 13300.0 Hydrogen 30 (S) 9 FTQKINSTW (F) 9 TFnEAKPIGF F/T|Q/K/I|N|S|T\W 100.0 9 5 7 4 0 dual b/y bias 45.606949 0.869217 562.7874 1124.573 -0.0068 -6.0 1.0 8.75 8763.2 nuORFdb_human ENST00000555408.5_1_12:56361645-56362592:+ ENST00000555408.5_1_12:56361645-56362592:+ CDK2 3' dORF 3' dORF GN=CDK2 3' dORF E_20221201_NO30_400nL_HLAc1_5e6_directIP_titration_rep1_Slot1-37_1_3502 5mio-rep2 E_20221201_NO30_400nL_HLAc1_5e6_directIP_titration_rep2_Slot2-2_1_3510.13118.1426.2 2 1 1 8.7 3.8 5.42 4.9 56.5 7 1 1 9960.0 Hydrogen 30 (S) 9 FTQKINSTW (F) 9 TFnEAKPIGF F/T|Q/K I\N\S|T\W 77.8 7 5 4 2 0 unbiased 45.308151 0.86029 562.7856 1124.573 -0.0088 -7.8 0.8999999999999995 8.75 8763.2 nuORFdb_human ENST00000555408.5_1_12:56361645-56362592:+ ENST00000555408.5_1_12:56361645-56362592:+ CDK2 3' dORF 3' dORF GN=CDK2 3' dORF E_20221201_NO30_400nL_HLAc1_5e6_directIP_titration_rep2_Slot2-2_1_3510 5mio-rep3 E_20221201_NO30_400nL_HLAc1_5e6_directIP_titration_rep3_Slot2-2_1_3511.13132.1430.2 2 1 1 10.78 3.67 6.0 7.1 68.1 7 1 1 6920.0 Hydrogen 30 (S) 9 FTQKINSTW (F) 9 TAATAPPAAPAGE F/T/Q/K I\N|S|T\W 77.8 7 4 5 2 0 unbiased 45.364109 0.857606 562.7873 1124.573 -0.0068 -6.0 2.1999999999999993 8.75 8763.2 nuORFdb_human ENST00000555408.5_1_12:56361645-56362592:+ ENST00000555408.5_1_12:56361645-56362592:+ CDK2 3' dORF 3' dORF GN=CDK2 3' dORF E_20221201_NO30_400nL_HLAc1_5e6_directIP_titration_rep3_Slot2-2_1_3511 SCP E_20221201_NO30_400nL_HLAc1_4e7_PDAC_Slot2-6_1_3524.29542.1180.2 2 1 1 20.37 10.11 12.75 10.26 95.4 10 1 1 5510.0 Hydrogen 48 (H) 11 FYLPDFYFGKF (R) 11 FYLPRPRSmSSS F/Y|L|P|D|F|Y|F/G-K\F 100.0 11 7 8 6 1 dual b/y bias 102.066414 1.013141 722.3528 1443.698 0.0004 0.3 2.6999999999999997 5.83 8561.0 nuORFdb_human ENST00000278612.8_1_11:108068056-108093342:- ENST00000278612.8_1_11:108068056-108093342:- NPAT 3' Overlap dORF 3' Overlap dORF GN=NPAT 3' Overlap dORF E_20221201_NO30_400nL_HLAc1_4e7_PDAC_Slot2-6_1_3524 SCP E_20221201_NO30_400nL_HLAc1_4e7_PDAC_Slot2-6_1_3524.25906.1312.2 2 1 1 13.7 7.51 8.58 6.18 80.1 7 1 1 8890.0 Hydrogen 10 (I) 9 FYSIEFKQF (R) 9 FYSLRHIASD F/Y|S|I/E|F-K Q\F 77.8 7 4 5 3 1 dual b/y bias 89.497269 0.931397 604.8032 1208.599 0.0001 0.1 2.5 6.0 3827.5 nuORFdb_human ENST00000354105.8_1_10:102020785-102021812:- ENST00000354105.8_1_10:102020785-102021812:-|ENST00000468709.5_1_10:102020754-102021812:- CWF19L1 Out-of-Frame Out-of-Frame GN=CWF19L1 Out-of-Frame E_20221201_NO30_400nL_HLAc1_4e7_PDAC_Slot2-6_1_3524 Eva-50mio-3fr AL20210827_EV_HLA_PDAC_PANFRO413_2D_50e6_W632_classI_fxn3.37783.37783.2 2 1 1 9.48 4.98 6.78 4.5 65.6 5 0 0 6080000.0 Hydrogen 678 (L) 9 FYSNIQTVI (N) 9 NYSFLAASVL F Y\S N\I\Q T/V/I 33.3 3 3 2 0 0 unbiased 0.892 77.9 68.78 74.15 0.0 542.7873 1084.567 -0.0 -0.0 -0.5 5.52 213870.4 nuORFdb_human ENST00000617998.4_2_17:7388053-7417318:+ ENST00000617998.4_2_17:7388053-7417318:+ POLR2A 5' Overlap uORF 5' Overlap uORF GN=POLR2A 5' Overlap uORF AL20210827_EV_HLA_PDAC_PANFRO413_2D_50e6_W632_classI_fxn3 Exploris_FAIMS VH20221207_MKP_FAIMS2022E_HLAc1_4e7_PDAC.55805.55805.2 2 1 1 12.4 5.72 7.89 6.68 78.6 7 0 0 11800000.0 Hydrogen 678 (L) 9 FYSNIQTVI (N) 9 LFYSNIQTV F Y\S\N\I\Q-T/V/I 77.8 7 4 2 0 1 b-ion bias 0.778 86.7 97.6 26.85 -50.0 542.7859 1084.567 -0.0028 -2.6 0.0 5.52 213870.4 nuORFdb_human ENST00000617998.4_2_17:7388053-7417318:+ ENST00000617998.4_2_17:7388053-7417318:+ POLR2A 5' Overlap uORF 5' Overlap uORF GN=POLR2A 5' Overlap uORF VH20221207_MKP_FAIMS2022E_HLAc1_4e7_PDAC SCP E_20221201_NO30_400nL_HLAc1_4e7_PDAC_Slot2-6_1_3524.12767.676.1 1 4 1 10.05 4.53 5.76 5.51 67.6 8 0 0 53200.0 Hydrogen 59 (P) 10 GAAAAAAPLA (A) 10 AAGLPGVAGA G A/A/A/A|A|A/P-L\A 80.0 8 3 6 2 1 y-ion bias 44.107998 1.320668 783.4328 783.436 -0.0029 -3.7 -1.3000000000000003 5.52 12556.0 nuORFdb_human ENST00000371711.4_1_20:48273160-48330387:- ENST00000371711.4_1_20:48273160-48330387:- B4GALT5 5' Overlap uORF 5' Overlap uORF GN=B4GALT5 5' Overlap uORF E_20221201_NO30_400nL_HLAc1_4e7_PDAC_Slot2-6_1_3524 SCP E_20221201_NO30_400nL_HLAc1_4e7_PDAC_Slot2-6_1_3524.21256.519.1 1 1 1 8.61 4.36 4.53 4.25 61.3 6 0 0 7320.0 Hydrogen 83 (P) 9 GAAAVQLLY (R) 9 ALQGALGLY G A A/A|V|Q\L\L\Y 66.7 6 5 3 2 0 unbiased 73.431152 1.414774 905.508 905.509 -0.0011 -1.2 1.2 5.52 12248.8 nuORFdb_human TCONS_00025884_17:27894303-27895505:+ TCONS_00025884_17:27894303-27895505:+|TCONS_00025885_17:27894992-27895505:+|ENST00000578749.5_2_17:27895268-27895505:+|TCONS_00025886_17:27895125-27895505:+ linc-TP53I13 5' uORF 5' uORF GN=linc-TP53I13 5' uORF E_20221201_NO30_400nL_HLAc1_4e7_PDAC_Slot2-6_1_3524 10mio-rep1 E_20221201_NO30_400nL_HLAc1_1e7_directIP_titration_rep1_Slot2-3_1_3512.21660.695.1 1 1 1 8.82 6.71 4.49 2.12 73.5 6 0 0 1900.0 Hydrogen 81 (W) 8 GAADPLTW (I) 8 GAGELPTW G A/A/D/P/L|T\W 75.0 6 2 5 1 0 y-ion bias 74.819283 1.309491 830.3996 830.404 -0.0043 -5.2 2.5 3.8 11475.7 smORF_v4|nuORFdb_human LINC00673_17:70588378-70588704:- LINC00673_17:70588378-70588704:-|TCONS_00025114_17:70588377-70588776:- LINC00673 lncRNA 17:70588378-70588704:- 17 linc-ABCA5-3 lincRNA lncRNA GN=LINC00673 E_20221201_NO30_400nL_HLAc1_1e7_directIP_titration_rep1_Slot2-3_1_3512 10mio-rep3 E_20221201_NO30_400nL_HLAc1_1e7_directIP_titration_rep3_Slot2-3_1_3514.21539.704.1 1 1 1 8.94 6.07 4.99 2.87 76.6 6 0 0 1860.0 Hydrogen 81 (W) 8 GAADPLTW (I) 8 GAGELPTW G A/A/D|P\L|T\W 75.0 6 4 4 2 0 unbiased 74.400047 1.303869 830.3992 830.404 -0.0053 -6.4 -0.10000000000000053 3.8 11475.7 smORF_v4|nuORFdb_human LINC00673_17:70588378-70588704:- LINC00673_17:70588378-70588704:-|TCONS_00025114_17:70588377-70588776:- LINC00673 lncRNA 17:70588378-70588704:- 17 linc-ABCA5-3 lincRNA lncRNA GN=LINC00673 E_20221201_NO30_400nL_HLAc1_1e7_directIP_titration_rep3_Slot2-3_1_3514 40mio-rep3 E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep3_Slot2-5_1_3521.21467.681.1 1 3 1 10.45 6.88 6.96 3.58 85.8 6 0 0 12800.0 Hydrogen 81 (W) 8 GAADPLTW (I) 8 EANPLTW G A/A/D|P\L|T\W 75.0 6 4 4 2 0 unbiased 74.157837 1.317932 830.3984 830.404 -0.0063 -7.6 -3.0 3.8 11475.7 smORF_v4|nuORFdb_human LINC00673_17:70588378-70588704:- LINC00673_17:70588378-70588704:-|TCONS_00025114_17:70588377-70588776:- LINC00673 lncRNA 17:70588378-70588704:- 17 linc-ABCA5-3 lincRNA lncRNA GN=LINC00673 E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep3_Slot2-5_1_3521 40mio-rep4 E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep4_Slot2-5_1_3522.21468.677.1 1 2 1 10.41 7.17 5.89 3.24 75.7 6 0 0 9710.0 Hydrogen 81 (W) 8 GAADPLTW (I) 8 EANPLTW G A/A/D|P\L|T\W 75.0 6 4 4 2 0 unbiased 74.143166 1.31986 830.3999 830.404 -0.0043 -5.2 -0.5 3.8 11475.7 smORF_v4|nuORFdb_human LINC00673_17:70588378-70588704:- LINC00673_17:70588378-70588704:-|TCONS_00025114_17:70588377-70588776:- LINC00673 lncRNA 17:70588378-70588704:- 17 linc-ABCA5-3 lincRNA lncRNA GN=LINC00673 E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep4_Slot2-5_1_3522 40mio-rep2 E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep2_Slot2-5_1_3520.29811.166.1 1 1 1 8.37 3.69 7.09 4.67 69.2 6 0 0 1520.0 Hydrogen 81 (W) 11 GAADPLTWITW (C) 11 WGAADPLTWIT G A A D/P L/T|W\I\T\W 45.5 5 4 3 1 0 unbiased 102.982391 1.622714 1230.6129 1230.615 -0.0054 -4.3 0.6000000000000005 3.8 11475.7 smORF_v4|nuORFdb_human LINC00673_17:70588378-70588704:- LINC00673_17:70588378-70588704:-|TCONS_00025114_17:70588377-70588776:- LINC00673 lncRNA 17:70588378-70588704:- 17 linc-ABCA5-3 lincRNA lncRNA GN=LINC00673 E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep2_Slot2-5_1_3520 40mio-rep3 E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep3_Slot2-5_1_3521.29811.1359.2 2 1 1 9.27 4.18 6.3 5.09 70.3 7 0 0 1870.0 Hydrogen 81 (W) 11 GAADPLTWITW (C) 11 EPGVKnWLTW G A A D/P\L|T|W|I|T\W 63.6 7 6 5 4 0 dual b/y bias 102.974144 0.902317 615.8111 1230.615 -0.0006 -0.5 4.1 3.8 11475.7 smORF_v4|nuORFdb_human LINC00673_17:70588378-70588704:- LINC00673_17:70588378-70588704:-|TCONS_00025114_17:70588377-70588776:- LINC00673 lncRNA 17:70588378-70588704:- 17 linc-ABCA5-3 lincRNA lncRNA GN=LINC00673 E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep3_Slot2-5_1_3521 Exploris_FAIMS VH20221207_MKP_FAIMS2022E_HLAc1_4e7_PDAC.37554.37666.2 2 1 1 7.86 6.45 1.33 1.41 68.0 6 0 0 292000000.0 Hydrogen 62 (S) 11 GASPLPTSQAG (A) 11 QSPTGPLGTAG G A S\P\L\P\T|S Q-A G 36.4 4 5 1 1 1 b-ion bias 0.998 97.7 70.1 9.25 -70.0 493.252 985.495 0.0018 1.8 4.4 5.52 11085.1 nuORFdb_human ENST00000392701.7_1_2:166650648-166650972:- ENST00000392701.7_1_2:166650648-166650972:- GALNT3 5' uORF 5' uORF GN=GALNT3 5' uORF VH20221207_MKP_FAIMS2022E_HLAc1_4e7_PDAC 10mio-rep1 E_20221201_NO30_400nL_HLAc1_1e7_directIP_titration_rep1_Slot2-3_1_3512.25188.528.1 1 1 1 7.34 5.23 2.85 2.11 66.9 6 0 0 2990.0 Hydrogen 66 (S) 9 GAWDALAEV (-) 9 LGSGGNQADI G A/W D-A\L\A\E\V 55.6 5 4 1 0 1 b-ion bias 87.002159 1.409368 931.4455 931.452 -0.006 -6.4 1.2999999999999998 3.66 8133.1 nuORFdb_human ENST00000378827.4_1_20:6748503-6748725:+ ENST00000378827.4_1_20:6748503-6748725:+ BMP2 5' uORF 5' uORF GN=BMP2 5' uORF E_20221201_NO30_400nL_HLAc1_1e7_directIP_titration_rep1_Slot2-3_1_3512 20mio-rep1 E_20221201_NO30_400nL_HLAc1_2e7_directIP_titration_rep1_Slot2-4_1_3515.25062.524.1 1 1 1 7.2 4.63 1.76 2.58 65.4 5 0 0 6490.0 Hydrogen 66 (S) 9 GAWDALAEV (-) 9 EAAYHELV G A W D\A\L\A\E\V 55.6 5 5 0 0 0 b-ion bias 86.544609 1.411836 931.4444 931.452 -0.008 -8.6 -2.3999999999999995 3.66 8133.1 nuORFdb_human ENST00000378827.4_1_20:6748503-6748725:+ ENST00000378827.4_1_20:6748503-6748725:+ BMP2 5' uORF 5' uORF GN=BMP2 5' uORF E_20221201_NO30_400nL_HLAc1_2e7_directIP_titration_rep1_Slot2-4_1_3515 20mio-rep2 E_20221201_NO30_400nL_HLAc1_2e7_directIP_titration_rep3_Slot2-4_1_3518.25085.518.1 1 2 1 7.81 4.22 2.18 3.59 69.3 5 0 0 8010.0 Hydrogen 66 (S) 9 GAWDALAEV (-) 9 LGSGGNQADI G A W D\A\L\A\E\V 55.6 5 5 0 0 0 b-ion bias 86.642723 1.415527 931.4449 931.452 -0.007 -7.5 -1.2000000000000002 3.66 8133.1 nuORFdb_human ENST00000378827.4_1_20:6748503-6748725:+ ENST00000378827.4_1_20:6748503-6748725:+ BMP2 5' uORF 5' uORF GN=BMP2 5' uORF E_20221201_NO30_400nL_HLAc1_2e7_directIP_titration_rep3_Slot2-4_1_3518 20mio-rep3 E_20221201_NO30_400nL_HLAc1_2e7_directIP_titration_rep4_Slot2-4_1_3519.25097.521.1 1 2 1 7.59 4.03 3.33 3.56 66.1 5 0 0 5220.0 Hydrogen 66 (S) 9 GAWDALAEV (-) 9 LNEAWADL G A W D\A\L\A\E\V 55.6 5 5 0 0 0 b-ion bias 86.675758 1.413253 931.4472 931.452 -0.005 -5.3 0.5 3.66 8133.1 nuORFdb_human ENST00000378827.4_1_20:6748503-6748725:+ ENST00000378827.4_1_20:6748503-6748725:+ BMP2 5' uORF 5' uORF GN=BMP2 5' uORF E_20221201_NO30_400nL_HLAc1_2e7_directIP_titration_rep4_Slot2-4_1_3519 20mio-rep2 E_20221201_NO30_400nL_HLAc1_2e7_directIP_titration_rep3_Slot2-4_1_3518.16864.694.1 1 1 1 8.38 5.45 3.83 2.93 76.9 6 0 0 2080.0 Hydrogen 14 (C) 9 GAWSGPPGV (-) 9 GWAGSPGPV G A/W/S|G/P|P G\V 55.6 5 3 5 2 0 unbiased 58.249264 1.31014 827.3986 827.405 -0.0056 -6.8 -0.5 5.52 2292.5 nuORFdb_human ENST00000263228.3_1_9:33817566-33817632:+ ENST00000263228.3_1_9:33817566-33817632:+ UBE2R2 5' uORF 5' uORF GN=UBE2R2 5' uORF E_20221201_NO30_400nL_HLAc1_2e7_directIP_titration_rep3_Slot2-4_1_3518 20mio-rep3 E_20221201_NO30_400nL_HLAc1_2e7_directIP_titration_rep4_Slot2-4_1_3519.16829.692.1 1 3 1 7.96 5.62 3.84 2.34 80.5 6 0 0 5830.0 Hydrogen 14 (C) 9 GAWSGPPGV (-) 9 GWAGSPGPV G A/W/S/G/P\P G\V 55.6 5 2 4 0 0 y-ion bias 58.127373 1.311074 827.3969 827.405 -0.0076 -9.2 -3.3999999999999995 5.52 2292.5 nuORFdb_human ENST00000263228.3_1_9:33817566-33817632:+ ENST00000263228.3_1_9:33817566-33817632:+ UBE2R2 5' uORF 5' uORF GN=UBE2R2 5' uORF E_20221201_NO30_400nL_HLAc1_2e7_directIP_titration_rep4_Slot2-4_1_3519 40mio-rep1 E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep1_Slot1-04_1_3496.17062.677.1 1 3 1 12.6 8.88 6.67 3.72 85.5 7 0 0 15600.0 Hydrogen 14 (C) 9 GAWSGPPGV (-) 9 GWAGSPGPV G A/W/S|G|P|P\G\V 77.8 7 5 5 3 0 dual b/y bias 58.93647 1.320099 827.3991 827.405 -0.0056 -6.8 -3.5999999999999996 5.52 2292.5 nuORFdb_human ENST00000263228.3_1_9:33817566-33817632:+ ENST00000263228.3_1_9:33817566-33817632:+ UBE2R2 5' uORF 5' uORF GN=UBE2R2 5' uORF E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep1_Slot1-04_1_3496 40mio-rep2 E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep2_Slot2-5_1_3520.16758.683.1 1 2 1 11.02 7.96 5.56 3.05 82.9 7 0 0 9190.0 Hydrogen 14 (C) 9 GAWSGPPGV (-) 9 GWAGSPGPV G A/W/S/G|P|P\G\V 77.8 7 4 5 2 0 unbiased 57.88776 1.316363 827.4007 827.405 -0.0036 -4.4 0.5 5.52 2292.5 nuORFdb_human ENST00000263228.3_1_9:33817566-33817632:+ ENST00000263228.3_1_9:33817566-33817632:+ UBE2R2 5' uORF 5' uORF GN=UBE2R2 5' uORF E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep2_Slot2-5_1_3520 40mio-rep3 E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep3_Slot2-5_1_3521.16760.687.1 1 1 1 10.35 6.82 6.39 3.53 80.1 7 0 0 3200.0 Hydrogen 14 (C) 9 GAWSGPPGV (-) 9 GWAGSPGPV G A/W/S/G|P|P\G\V 77.8 7 4 5 2 0 unbiased 57.89616 1.314003 827.4014 827.405 -0.0036 -4.4 0.1999999999999993 5.52 2292.5 nuORFdb_human ENST00000263228.3_1_9:33817566-33817632:+ ENST00000263228.3_1_9:33817566-33817632:+ UBE2R2 5' uORF 5' uORF GN=UBE2R2 5' uORF E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep3_Slot2-5_1_3521 40mio-rep4 E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep4_Slot2-5_1_3522.16742.681.1 1 1 1 13.97 9.64 7.91 4.32 90.5 7 0 0 3420.0 Hydrogen 14 (C) 9 GAWSGPPGV (-) 9 GWAGSPGPV G A/W/S|G/P|P\G\V 77.8 7 4 5 2 0 unbiased 57.818363 1.317548 827.3967 827.405 -0.0076 -9.2 -4.499999999999999 5.52 2292.5 nuORFdb_human ENST00000263228.3_1_9:33817566-33817632:+ ENST00000263228.3_1_9:33817566-33817632:+ UBE2R2 5' uORF 5' uORF GN=UBE2R2 5' uORF E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep4_Slot2-5_1_3522 10mio-rep1 E_20221201_NO30_400nL_HLAc1_1e7_directIP_titration_rep1_Slot2-3_1_3512.11023.1489.2 2 2 1 11.62 7.17 4.89 4.45 70.2 7 1 1 13500.0 Hydrogen 138 (N) 11 GEAALGGGRGW (G) 11 EGAALDPGTSL G E/A|A|L|G/G G\R\G-W 63.6 7 5 5 3 1 unbiased 38.082085 0.820223 515.7511 1030.506 -0.0117 -11.4 -3.7 6.0 24872.9 nuORFdb_human ENST00000568689.5_1_16:733170-734340:- ENST00000568689.5_1_16:733170-734340:-|ENST00000569441.5_1_16:733170-733950:- JMJD8 3' Overlap dORF 3' Overlap dORF GN=JMJD8 3' Overlap dORF E_20221201_NO30_400nL_HLAc1_1e7_directIP_titration_rep1_Slot2-3_1_3512 10mio-rep2 E_20221201_NO30_400nL_HLAc1_1e7_directIP_titration_rep2_Slot2-3_1_3513.10987.1478.2 2 1 1 14.95 8.58 7.5 6.37 83.0 8 1 1 4840.0 Hydrogen 138 (N) 11 GEAALGGGRGW (G) 11 EGAALDPGTSL G E/A|A|L|G/G|G\R\G-W 81.8 9 6 6 4 1 dual b/y bias 37.958992 0.827498 515.7512 1030.506 -0.0117 -11.4 -4.7 6.0 24872.9 nuORFdb_human ENST00000568689.5_1_16:733170-734340:- ENST00000568689.5_1_16:733170-734340:-|ENST00000569441.5_1_16:733170-733950:- JMJD8 3' Overlap dORF 3' Overlap dORF GN=JMJD8 3' Overlap dORF E_20221201_NO30_400nL_HLAc1_1e7_directIP_titration_rep2_Slot2-3_1_3513 20mio-rep1 E_20221201_NO30_400nL_HLAc1_2e7_directIP_titration_rep1_Slot2-4_1_3515.10916.1474.2 2 1 1 11.51 7.74 5.41 3.77 84.1 7 1 1 13200.0 Hydrogen 138 (N) 11 GEAALGGGRGW (G) 11 EGAALDPGTSL G E/A|A|L|G/G|G-R\G-W 81.8 9 5 6 4 2 dual b/y bias 37.703793 0.829873 515.7532 1030.506 -0.0077 -7.5 -1.2999999999999998 6.0 24872.9 nuORFdb_human ENST00000568689.5_1_16:733170-734340:- ENST00000568689.5_1_16:733170-734340:-|ENST00000569441.5_1_16:733170-733950:- JMJD8 3' Overlap dORF 3' Overlap dORF GN=JMJD8 3' Overlap dORF E_20221201_NO30_400nL_HLAc1_2e7_directIP_titration_rep1_Slot2-4_1_3515 20mio-rep2 E_20221201_NO30_400nL_HLAc1_2e7_directIP_titration_rep3_Slot2-4_1_3518.11083.1468.2 2 2 1 12.38 9.18 5.22 3.2 80.1 7 1 1 11700.0 Hydrogen 138 (N) 11 GEAALGGGRGW (G) 11 EGAALDPGTSL G/E/A|A|L|G/G G R\G-W 72.7 8 4 6 3 1 unbiased 38.281727 0.833566 515.7518 1030.506 -0.0097 -9.5 -3.2 6.0 24872.9 nuORFdb_human ENST00000568689.5_1_16:733170-734340:- ENST00000568689.5_1_16:733170-734340:-|ENST00000569441.5_1_16:733170-733950:- JMJD8 3' Overlap dORF 3' Overlap dORF GN=JMJD8 3' Overlap dORF E_20221201_NO30_400nL_HLAc1_2e7_directIP_titration_rep3_Slot2-4_1_3518 20mio-rep3 E_20221201_NO30_400nL_HLAc1_2e7_directIP_titration_rep4_Slot2-4_1_3519.10938.1460.2 2 1 1 13.23 10.04 5.29 3.19 76.6 7 1 1 7510.0 Hydrogen 138 (N) 11 GEAALGGGRGW (G) 11 EGAALDPGTSL G E/A|A|L|G/G\G-R\G-W 81.8 9 5 5 3 2 unbiased 37.786839 0.838959 515.7519 1030.506 -0.0097 -9.5 -3.7 6.0 24872.9 nuORFdb_human ENST00000568689.5_1_16:733170-734340:- ENST00000568689.5_1_16:733170-734340:-|ENST00000569441.5_1_16:733170-733950:- JMJD8 3' Overlap dORF 3' Overlap dORF GN=JMJD8 3' Overlap dORF E_20221201_NO30_400nL_HLAc1_2e7_directIP_titration_rep4_Slot2-4_1_3519 40mio-rep1 E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep1_Slot1-04_1_3496.11224.1436.2 2 1 1 17.65 13.08 7.92 4.58 88.7 9 1 1 30000.0 Hydrogen 138 (N) 11 GEAALGGGRGW (G) 11 EGAALDPGTSL G/E/A|A|L|G/G|G-R\G\W 100.0 11 6 7 4 1 dual b/y bias 38.772717 0.853969 515.7543 1030.506 -0.0057 -5.6 -2.3999999999999995 6.0 24872.9 nuORFdb_human ENST00000568689.5_1_16:733170-734340:- ENST00000568689.5_1_16:733170-734340:-|ENST00000569441.5_1_16:733170-733950:- JMJD8 3' Overlap dORF 3' Overlap dORF GN=JMJD8 3' Overlap dORF E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep1_Slot1-04_1_3496 40mio-rep2 E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep2_Slot2-5_1_3520.10916.1451.2 2 1 1 11.34 9.66 4.9 1.68 80.7 6 1 1 19500.0 Hydrogen 138 (N) 11 GEAALGGGRGW (G) 11 EGAALDPGTSL G E/A|A|L|G/G|G-R G W 54.5 6 4 6 4 1 dual b/y bias 37.710396 0.844665 515.755 1030.506 -0.0037 -3.6 1.3000000000000003 6.0 24872.9 nuORFdb_human ENST00000568689.5_1_16:733170-734340:- ENST00000568689.5_1_16:733170-734340:-|ENST00000569441.5_1_16:733170-733950:- JMJD8 3' Overlap dORF 3' Overlap dORF GN=JMJD8 3' Overlap dORF E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep2_Slot2-5_1_3520 40mio-rep3 E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep3_Slot2-5_1_3521.10921.1460.2 2 1 1 12.0 9.84 4.56 2.15 84.1 6 1 1 10900.0 Hydrogen 138 (N) 11 GEAALGGGRGW (G) 11 EGAALDPGTSL G E/A|A|L|G/G/G R G W 45.5 5 3 6 3 0 y-ion bias 37.730312 0.83901 515.7537 1030.506 -0.0057 -5.6 -1.0 6.0 24872.9 nuORFdb_human ENST00000568689.5_1_16:733170-734340:- ENST00000568689.5_1_16:733170-734340:-|ENST00000569441.5_1_16:733170-733950:- JMJD8 3' Overlap dORF 3' Overlap dORF GN=JMJD8 3' Overlap dORF E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep3_Slot2-5_1_3521 20mio-rep1 E_20221201_NO30_400nL_HLAc1_2e7_directIP_titration_rep1_Slot2-4_1_3515.20173.1434.2 2 1 1 7.29 3.74 2.88 3.55 62.8 6 1 1 4420.0 Hydrogen 14 (A) 10 GEAEPLLKAW (L) 10 nAAELVSGRPV G E/A/E|P/L/L K A-W 50.0 5 1 5 1 1 y-ion bias 69.666039 0.855339 557.2966 1113.594 -0.0072 -6.4 -0.20000000000000018 4.53 3993.8 nuORFdb_human ENST00000567109.5_1_16:82660554-82660662:+ ENST00000567109.5_1_16:82660554-82660662:+ CDH13 5' uORF 5' uORF GN=CDH13 5' uORF E_20221201_NO30_400nL_HLAc1_2e7_directIP_titration_rep1_Slot2-4_1_3515 20mio-rep2 E_20221201_NO30_400nL_HLAc1_2e7_directIP_titration_rep3_Slot2-4_1_3518.20186.1426.2 2 1 1 13.49 6.66 5.29 6.84 81.2 8 1 1 4750.0 Hydrogen 14 (A) 10 GEAEPLLKAW (L) 10 nAAELVSGRPV G E/A|E|P/L|L|K\A\W 80.0 8 6 6 4 0 dual b/y bias 69.727463 0.859972 557.2964 1113.594 -0.0092 -8.2 -1.8999999999999995 4.53 3993.8 nuORFdb_human ENST00000567109.5_1_16:82660554-82660662:+ ENST00000567109.5_1_16:82660554-82660662:+ CDH13 5' uORF 5' uORF GN=CDH13 5' uORF E_20221201_NO30_400nL_HLAc1_2e7_directIP_titration_rep3_Slot2-4_1_3518 20mio-rep3 E_20221201_NO30_400nL_HLAc1_2e7_directIP_titration_rep4_Slot2-4_1_3519.20224.1426.2 2 1 1 7.71 2.8 4.31 4.92 57.2 7 1 1 4620.0 Hydrogen 14 (A) 10 GEAEPLLKAW (L) 10 qSSTPLLKDAA G E/A/E/P/L\L|K\A W 60.0 6 3 5 1 0 unbiased 69.84861 0.860161 557.2977 1113.594 -0.0052 -4.6 1.2000000000000002 4.53 3993.8 nuORFdb_human ENST00000567109.5_1_16:82660554-82660662:+ ENST00000567109.5_1_16:82660554-82660662:+ CDH13 5' uORF 5' uORF GN=CDH13 5' uORF E_20221201_NO30_400nL_HLAc1_2e7_directIP_titration_rep4_Slot2-4_1_3519 40mio-rep1 E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep1_Slot1-04_1_3496.20348.1396.2 2 2 1 14.67 7.19 7.48 7.48 75.9 8 1 1 16700.0 Hydrogen 14 (A) 10 GEAEPLLKAW (L) 10 nAAELVSGRPV G E/A|E|P/L/L|K\A\W 80.0 8 5 6 3 0 unbiased 70.29216 0.87905 557.3005 1113.594 0.0008 0.8 4.0 4.53 3993.8 nuORFdb_human ENST00000567109.5_1_16:82660554-82660662:+ ENST00000567109.5_1_16:82660554-82660662:+ CDH13 5' uORF 5' uORF GN=CDH13 5' uORF E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep1_Slot1-04_1_3496 10mio-rep2 E_20221201_NO30_400nL_HLAc1_1e7_directIP_titration_rep2_Slot2-3_1_3513.12657.1559.2 2 1 1 9.06 4.49 3.57 4.57 74.5 6 0 1 1460.0 Hydrogen 163 (D) 9 GEARPLAGF (R) 9 PPSRVSAGF G E/A/R/P L|A\G\F 55.6 5 3 4 1 0 unbiased 43.72744 0.776271 459.2423 917.484 -0.0072 -7.9 -1.2000000000000002 6.0 18686.6 nuORFdb_human ENST00000528220.1_1_11:65487749-65488268:- ENST00000528220.1_1_11:65487749-65488268:-|ENST00000308418.8_1_11:65487749-65488293:- RNASEH2C 5' Overlap uORF 5' Overlap uORF GN=RNASEH2C 5' Overlap uORF E_20221201_NO30_400nL_HLAc1_1e7_directIP_titration_rep2_Slot2-3_1_3513 20mio-rep2 E_20221201_NO30_400nL_HLAc1_2e7_directIP_titration_rep3_Slot2-4_1_3518.12736.1549.2 2 1 1 11.15 6.54 3.57 4.61 78.8 6 0 1 2960.0 Hydrogen 163 (D) 9 GEARPLAGF (R) 9 PDPKVSAGF G E/A|R|P L|A|G\F 55.6 5 5 5 4 0 dual b/y bias 43.991447 0.782716 459.2437 917.484 -0.0032 -3.5 2.8 6.0 18686.6 nuORFdb_human ENST00000528220.1_1_11:65487749-65488268:- ENST00000528220.1_1_11:65487749-65488268:-|ENST00000308418.8_1_11:65487749-65488293:- RNASEH2C 5' Overlap uORF 5' Overlap uORF GN=RNASEH2C 5' Overlap uORF E_20221201_NO30_400nL_HLAc1_2e7_directIP_titration_rep3_Slot2-4_1_3518 40mio-rep1 E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep1_Slot1-04_1_3496.12901.1532.2 2 1 1 12.36 7.22 4.67 5.14 85.5 6 0 1 4920.0 Hydrogen 163 (D) 9 GEARPLAGF (R) 9 EAARPVAGF G E/A/R|P L|A|G\F 55.6 5 4 5 3 0 dual b/y bias 44.564182 0.793493 459.2437 917.484 -0.0032 -3.5 -0.2999999999999998 6.0 18686.6 nuORFdb_human ENST00000528220.1_1_11:65487749-65488268:- ENST00000528220.1_1_11:65487749-65488268:-|ENST00000308418.8_1_11:65487749-65488293:- RNASEH2C 5' Overlap uORF 5' Overlap uORF GN=RNASEH2C 5' Overlap uORF E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep1_Slot1-04_1_3496 40mio-rep2 E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep2_Slot2-5_1_3520.12580.1540.2 2 1 1 9.45 5.09 4.07 4.36 75.2 6 0 1 6640.0 Hydrogen 163 (D) 9 GEARPLAGF (R) 9 PPSRVSAGF G E/A/R|P L|A|G\F 55.6 5 4 5 3 0 dual b/y bias 43.457813 0.788457 459.2431 917.484 -0.0052 -5.7 -0.7999999999999998 6.0 18686.6 nuORFdb_human ENST00000528220.1_1_11:65487749-65488268:- ENST00000528220.1_1_11:65487749-65488268:-|ENST00000308418.8_1_11:65487749-65488293:- RNASEH2C 5' Overlap uORF 5' Overlap uORF GN=RNASEH2C 5' Overlap uORF E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep2_Slot2-5_1_3520 40mio-rep3 E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep3_Slot2-5_1_3521.12593.1546.2 2 1 1 11.41 7.42 4.12 3.99 83.3 6 0 1 7390.0 Hydrogen 163 (D) 9 GEARPLAGF (R) 9 EAARPVAGF G E/A/R|P L|A|G\F 55.6 5 4 5 3 0 dual b/y bias 43.506882 0.784236 459.2435 917.484 -0.0052 -5.7 -1.1000000000000005 6.0 18686.6 nuORFdb_human ENST00000528220.1_1_11:65487749-65488268:- ENST00000528220.1_1_11:65487749-65488268:-|ENST00000308418.8_1_11:65487749-65488293:- RNASEH2C 5' Overlap uORF 5' Overlap uORF GN=RNASEH2C 5' Overlap uORF E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep3_Slot2-5_1_3521 40mio-rep4 E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep4_Slot2-5_1_3522.12593.1543.2 2 2 1 10.18 5.02 3.6 5.15 80.9 6 0 1 11000.0 Hydrogen 163 (D) 9 GEARPLAGF (R) 9 EAARPVAGF G E/A/R|P L|A|G\F 55.6 5 4 5 3 0 dual b/y bias 43.487988 0.786358 459.2435 917.484 -0.0052 -5.7 -1.0 6.0 18686.6 nuORFdb_human ENST00000528220.1_1_11:65487749-65488268:- ENST00000528220.1_1_11:65487749-65488268:-|ENST00000308418.8_1_11:65487749-65488293:- RNASEH2C 5' Overlap uORF 5' Overlap uORF GN=RNASEH2C 5' Overlap uORF E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep4_Slot2-5_1_3522 40mio-rep2 E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep2_Slot2-5_1_3520.4659.1636.3 3 1 1 11.87 10.45 0.92 1.42 92.5 6 1 2 9930.0 Hydrogen 2 (L) 10 GEEGEATRRW (L) 10 qEGLAGYQREG G/E/E|G/E/A T R R\W 60.0 6 2 5 1 0 y-ion bias 16.103329 0.72764 397.5208 1190.555 -0.0064 -5.4 -0.5 4.79 2348.5 nuORFdb_human ENST00000314117.8_1_4:37687875-37687938:- ENST00000314117.8_1_4:37687875-37687938:- RELL1 5' Overlap uORF 5' Overlap uORF GN=RELL1 5' Overlap uORF E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep2_Slot2-5_1_3520 40mio-rep4 E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep4_Slot2-5_1_3522.4699.1625.3 3 1 1 9.49 7.21 1.8 2.28 85.7 7 1 2 34200.0 Hydrogen 2 (L) 10 GEEGEATRRW (L) 10 GEEGSRREEAA G/E/E|G/E/A T R-R\W 70.0 7 2 5 1 1 y-ion bias 16.236099 0.734188 397.5214 1190.555 -0.0064 -5.4 -0.7000000000000002 4.79 2348.5 nuORFdb_human ENST00000314117.8_1_4:37687875-37687938:- ENST00000314117.8_1_4:37687875-37687938:- RELL1 5' Overlap uORF 5' Overlap uORF GN=RELL1 5' Overlap uORF E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep4_Slot2-5_1_3522 20mio-rep3 E_20221201_NO30_400nL_HLAc1_2e7_directIP_titration_rep4_Slot2-4_1_3519.18424.1369.2 2 1 1 9.94 6.84 4.76 3.09 78.1 5 1 1 4950.0 Hydrogen 36 (K) 11 GEFGGFYSVRG (K) 11 FDANFTFKEG G E/F|G|G|F\Y S V R G 36.4 4 4 4 3 0 unbiased 63.633171 0.895845 588.2734 1175.548 -0.0093 -7.9 -2.1000000000000005 6.0 19423.1 nuORFdb_human ENST00000398588.2_1_6:58446590-58447106:- ENST00000398588.2_1_6:58446590-58447106:- RBBP4P4 Pseudogene Pseudogene GN=RBBP4P4 Pseudogene E_20221201_NO30_400nL_HLAc1_2e7_directIP_titration_rep4_Slot2-4_1_3519 40mio-rep4 E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep4_Slot2-5_1_3522.18452.1357.2 2 3 1 9.19 5.04 5.19 4.15 64.7 6 1 1 16400.0 Hydrogen 36 (K) 11 GEFGGFYSVRG (K) 11 FDANFTFKEG G E/F|G|G|F\Y/S V R G 45.5 5 4 5 3 0 unbiased 63.724777 0.902962 588.2753 1175.548 -0.0053 -4.5 0.20000000000000018 6.0 19423.1 nuORFdb_human ENST00000398588.2_1_6:58446590-58447106:- ENST00000398588.2_1_6:58446590-58447106:- RBBP4P4 Pseudogene Pseudogene GN=RBBP4P4 Pseudogene E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep4_Slot2-5_1_3522 10mio-rep2 E_20221201_NO30_400nL_HLAc1_1e7_directIP_titration_rep2_Slot2-3_1_3513.19006.1437.2 2 2 1 11.33 7.97 2.28 3.36 78.6 6 1 1 12000.0 Hydrogen 1336 (D) 9 GEFKALQEW (I) 9 SGVMSAIKEW G E/F|K A/L|Q|E\W 55.6 5 4 5 3 0 dual b/y bias 65.65065 0.853246 554.2737 1107.547 -0.0062 -5.6 1.1000000000000005 4.53 213870.4 nuORFdb_human ENST00000617998.4_2_17:7388053-7417318:+ ENST00000617998.4_2_17:7388053-7417318:+ POLR2A 5' Overlap uORF 5' Overlap uORF GN=POLR2A 5' Overlap uORF E_20221201_NO30_400nL_HLAc1_1e7_directIP_titration_rep2_Slot2-3_1_3513 10mio-rep3 E_20221201_NO30_400nL_HLAc1_1e7_directIP_titration_rep3_Slot2-3_1_3514.18947.1431.2 2 3 1 14.51 8.32 4.49 6.2 89.2 8 1 1 12800.0 Hydrogen 1336 (D) 9 GEFKALQEW (I) 9 SGVMSAIKEW G/E/F|K/A/L\Q|E\W 100.0 9 4 6 2 0 unbiased 65.448669 0.85694 554.274 1107.547 -0.0062 -5.6 0.7000000000000002 4.53 213870.4 nuORFdb_human ENST00000617998.4_2_17:7388053-7417318:+ ENST00000617998.4_2_17:7388053-7417318:+ POLR2A 5' Overlap uORF 5' Overlap uORF GN=POLR2A 5' Overlap uORF E_20221201_NO30_400nL_HLAc1_1e7_directIP_titration_rep3_Slot2-3_1_3514 20mio-rep1 E_20221201_NO30_400nL_HLAc1_2e7_directIP_titration_rep1_Slot2-4_1_3515.18974.1427.2 2 2 1 9.76 7.47 1.24 2.29 74.9 7 1 1 11800.0 Hydrogen 1336 (D) 9 GEFKALQEW (I) 9 SGVMSAIKEW G E/F|K/A/L|Q|E\W 77.8 7 4 6 3 0 dual b/y bias 65.526962 0.859649 554.2732 1107.547 -0.0082 -7.4 -1.2000000000000002 4.53 213870.4 nuORFdb_human ENST00000617998.4_2_17:7388053-7417318:+ ENST00000617998.4_2_17:7388053-7417318:+ POLR2A 5' Overlap uORF 5' Overlap uORF GN=POLR2A 5' Overlap uORF E_20221201_NO30_400nL_HLAc1_2e7_directIP_titration_rep1_Slot2-4_1_3515 20mio-rep2 E_20221201_NO30_400nL_HLAc1_2e7_directIP_titration_rep3_Slot2-4_1_3518.19042.1421.2 2 2 1 11.49 5.56 3.17 5.93 78.9 7 1 1 24600.0 Hydrogen 1336 (D) 9 GEFKALQEW (I) 9 FLSNALEQW G E/F|K/A/L|Q|E\W 77.8 7 4 6 3 0 dual b/y bias 65.771538 0.863352 554.274 1107.547 -0.0062 -5.6 0.7000000000000002 4.53 213870.4 nuORFdb_human ENST00000617998.4_2_17:7388053-7417318:+ ENST00000617998.4_2_17:7388053-7417318:+ POLR2A 5' Overlap uORF 5' Overlap uORF GN=POLR2A 5' Overlap uORF E_20221201_NO30_400nL_HLAc1_2e7_directIP_titration_rep3_Slot2-4_1_3518 20mio-rep3 E_20221201_NO30_400nL_HLAc1_2e7_directIP_titration_rep4_Slot2-4_1_3519.19019.1421.2 2 2 1 12.75 9.83 2.35 2.92 82.8 6 1 1 22200.0 Hydrogen 1336 (D) 9 GEFKALQEW (I) 9 SGVMSAIKEW G E/F|K/A L|Q|E\W 55.6 5 4 5 3 0 dual b/y bias 65.688263 0.862906 554.2727 1107.547 -0.0082 -7.4 -1.6000000000000005 4.53 213870.4 nuORFdb_human ENST00000617998.4_2_17:7388053-7417318:+ ENST00000617998.4_2_17:7388053-7417318:+ POLR2A 5' Overlap uORF 5' Overlap uORF GN=POLR2A 5' Overlap uORF E_20221201_NO30_400nL_HLAc1_2e7_directIP_titration_rep4_Slot2-4_1_3519 40mio-rep1 E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep1_Slot1-04_1_3496.19155.1385.2 2 3 1 13.08 7.37 4.82 5.71 88.5 6 1 1 56000.0 Hydrogen 1336 (D) 9 GEFKALQEW (I) 9 SGVMSAIKEW G E/F|K/A L\Q|E\W 55.6 5 4 4 2 0 unbiased 66.171448 0.885967 554.2758 1107.547 -0.0022 -2.0 1.2000000000000002 4.53 213870.4 nuORFdb_human ENST00000617998.4_2_17:7388053-7417318:+ ENST00000617998.4_2_17:7388053-7417318:+ POLR2A 5' Overlap uORF 5' Overlap uORF GN=POLR2A 5' Overlap uORF E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep1_Slot1-04_1_3496 40mio-rep2 E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep2_Slot2-5_1_3520.18903.1399.2 2 4 1 12.92 9.27 2.43 3.66 85.5 6 1 1 41200.0 Hydrogen 1336 (D) 9 GEFKALQEW (I) 9 SGVMSAIKEW G E/F|K/A L|Q|E\W 55.6 5 4 5 3 0 dual b/y bias 65.301437 0.877276 554.2746 1107.547 -0.0042 -3.8 1.1000000000000005 4.53 213870.4 nuORFdb_human ENST00000617998.4_2_17:7388053-7417318:+ ENST00000617998.4_2_17:7388053-7417318:+ POLR2A 5' Overlap uORF 5' Overlap uORF GN=POLR2A 5' Overlap uORF E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep2_Slot2-5_1_3520 40mio-rep3 E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep3_Slot2-5_1_3521.18946.1403.2 2 3 1 11.58 6.65 1.23 4.93 79.2 6 1 1 24000.0 Hydrogen 1336 (D) 9 GEFKALQEW (I) 9 FLSNALEQW G E/F|K/A L|Q|E\W 55.6 5 4 5 3 0 dual b/y bias 65.447174 0.874492 554.2744 1107.547 -0.0062 -5.6 -1.0 4.53 213870.4 nuORFdb_human ENST00000617998.4_2_17:7388053-7417318:+ ENST00000617998.4_2_17:7388053-7417318:+ POLR2A 5' Overlap uORF 5' Overlap uORF GN=POLR2A 5' Overlap uORF E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep3_Slot2-5_1_3521 40mio-rep4 E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep4_Slot2-5_1_3522.18927.1401.2 2 4 1 9.44 5.28 4.03 4.16 73.5 6 1 1 36500.0 Hydrogen 1336 (D) 9 GEFKALQEW (I) 9 SGVMSAIKEW G E/F|K/A L\Q|E\W 55.6 5 4 4 2 0 unbiased 65.364655 0.875462 554.2757 1107.547 -0.0022 -2.0 2.7 4.53 213870.4 nuORFdb_human ENST00000617998.4_2_17:7388053-7417318:+ ENST00000617998.4_2_17:7388053-7417318:+ POLR2A 5' Overlap uORF 5' Overlap uORF GN=POLR2A 5' Overlap uORF E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep4_Slot2-5_1_3522 5mio-rep1 E_20221201_NO30_400nL_HLAc1_5e6_directIP_titration_rep1_Slot1-37_1_3502.19255.1441.2 2 1 1 7.5 3.6 2.03 3.9 67.1 6 1 1 4820.0 Hydrogen 1336 (D) 9 GEFKALQEW (I) 9 FAKAIGDAEW G E/F|K A/L\Q|E\W 55.6 5 4 4 2 0 unbiased 66.506821 0.85059 554.2727 1107.547 -0.0082 -7.4 -0.40000000000000036 4.53 213870.4 nuORFdb_human ENST00000617998.4_2_17:7388053-7417318:+ ENST00000617998.4_2_17:7388053-7417318:+ POLR2A 5' Overlap uORF 5' Overlap uORF GN=POLR2A 5' Overlap uORF E_20221201_NO30_400nL_HLAc1_5e6_directIP_titration_rep1_Slot1-37_1_3502 10mio-rep1 E_20221201_NO30_400nL_HLAc1_1e7_directIP_titration_rep1_Slot2-3_1_3512.12209.833.1 1 2 1 8.59 2.63 5.75 5.96 72.4 6 0 0 3630.0 Hydrogen 22 (A) 9 GEGGGVAGF (L) 9 SNPASAGF G E/G G/G|V|A\G\F 55.6 5 4 4 2 0 unbiased 42.180153 1.226027 750.3335 750.342 -0.0077 -10.3 -2.6000000000000005 4.0 3829.3 nuORFdb_human ENST00000521611.1_1_8:53536025-53626720:- ENST00000521611.1_1_8:53536025-53626720:- RB1CC1 3' Overlap dORF 3' Overlap dORF GN=RB1CC1 3' Overlap dORF E_20221201_NO30_400nL_HLAc1_1e7_directIP_titration_rep1_Slot2-3_1_3512 40mio-rep1 E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep1_Slot1-04_1_3496.12425.811.1 1 1 1 10.45 4.1 6.31 6.36 79.1 6 0 0 4370.0 Hydrogen 22 (A) 9 GEGGGVAGF (L) 9 ANGEGVGF G E/G G/G|V\A\G\F 55.6 5 4 3 1 0 unbiased 42.920048 1.239031 750.3389 750.342 -0.0027 -3.6 -0.3999999999999999 4.0 3829.3 nuORFdb_human ENST00000521611.1_1_8:53536025-53626720:- ENST00000521611.1_1_8:53536025-53626720:- RB1CC1 3' Overlap dORF 3' Overlap dORF GN=RB1CC1 3' Overlap dORF E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep1_Slot1-04_1_3496 40mio-rep2 E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep2_Slot2-5_1_3520.12093.826.1 1 1 1 9.78 4.05 5.29 5.73 81.2 6 0 0 6820.0 Hydrogen 22 (A) 9 GEGGGVAGF (L) 9 SNPASAGF G E/G/G G|V\A\G\F 55.6 5 4 3 1 0 unbiased 41.773949 1.230334 750.3355 750.342 -0.0067 -8.9 -4.0 4.0 3829.3 nuORFdb_human ENST00000521611.1_1_8:53536025-53626720:- ENST00000521611.1_1_8:53536025-53626720:- RB1CC1 3' Overlap dORF 3' Overlap dORF GN=RB1CC1 3' Overlap dORF E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep2_Slot2-5_1_3520 20mio-rep1 E_20221201_NO30_400nL_HLAc1_2e7_directIP_titration_rep1_Slot2-4_1_3515.16364.716.1 1 2 1 10.16 5.88 2.57 4.28 80.1 6 0 0 3320.0 Hydrogen 98 (F) 9 GEGGVPAAW (R) 9 GSAPSAPAW G E/G/G V|P\A|A\W 55.6 5 4 4 2 0 unbiased 56.514999 1.296564 843.3929 843.399 -0.0065 -7.8 -1.5999999999999996 4.0 14801.8 nuORFdb_human ENST00000517503.1_3_8:144103316-144103727:+ ENST00000517503.1_3_8:144103316-144103727:+ LY6E 3' dORF 3' dORF GN=LY6E 3' dORF E_20221201_NO30_400nL_HLAc1_2e7_directIP_titration_rep1_Slot2-4_1_3515 40mio-rep4 E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep4_Slot2-5_1_3522.16334.693.1 1 1 1 10.09 7.02 2.04 3.07 81.2 5 0 0 2930.0 Hydrogen 98 (F) 9 GEGGVPAAW (R) 9 GSAPSAPAW G E G G|V|P\A|A\W 55.6 5 5 3 3 0 dual b/y bias 56.409096 1.310302 843.3954 843.399 -0.0045 -5.4 -0.7000000000000002 4.0 14801.8 nuORFdb_human ENST00000517503.1_3_8:144103316-144103727:+ ENST00000517503.1_3_8:144103316-144103727:+ LY6E 3' dORF 3' dORF GN=LY6E 3' dORF E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep4_Slot2-5_1_3522 40mio-rep1 E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep1_Slot1-04_1_3496.15640.1412.2 2 1 1 13.45 5.7 8.11 7.74 81.1 7 1 1 11400.0 Hydrogen 48 (N) 9 GEIKMTTSW (F) 9 EGNKGVTGSGF G E/I/K/M|T|T|S\W 77.8 7 4 6 3 0 dual b/y bias 54.025013 0.868707 526.7563 1052.508 -0.0034 -3.2 0.0 6.0 7035.9 nuORFdb_human ENST00000528848.2_1_11:11374530-11374710:- ENST00000528848.2_1_11:11374530-11374710:-|ENST00000609606.5_1_20:485838-489110:- CSNK2A3 5' Overlap uORF 5' Overlap uORF GN=CSNK2A3 5' Overlap uORF E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep1_Slot1-04_1_3496 40mio-rep3 E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep3_Slot2-5_1_3521.15341.1434.2 2 1 1 10.71 6.11 5.67 4.59 76.0 7 1 1 7980.0 Hydrogen 48 (N) 9 GEIKMTTSW (F) 9 GFSRLTADGE G E/I|K/M\T\T|S\W 77.8 7 5 4 2 0 unbiased 52.995216 0.855084 526.7566 1052.508 -0.0014 -1.3 3.3 6.0 7035.9 nuORFdb_human ENST00000528848.2_1_11:11374530-11374710:- ENST00000528848.2_1_11:11374530-11374710:-|ENST00000609606.5_1_20:485838-489110:- CSNK2A3 5' Overlap uORF 5' Overlap uORF GN=CSNK2A3 5' Overlap uORF E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep3_Slot2-5_1_3521 40mio-rep4 E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep4_Slot2-5_1_3522.15334.1433.2 2 1 1 10.63 3.86 5.54 6.78 75.0 7 1 1 8090.0 Hydrogen 48 (N) 9 GEIKMTTSW (F) 9 mnLKKESW G E/I|K/M\T|T|S\W 77.8 7 5 5 3 0 dual b/y bias 52.956482 0.855897 526.7551 1052.508 -0.0054 -5.1 -0.39999999999999947 6.0 7035.9 nuORFdb_human ENST00000528848.2_1_11:11374530-11374710:- ENST00000528848.2_1_11:11374530-11374710:-|ENST00000609606.5_1_20:485838-489110:- CSNK2A3 5' Overlap uORF 5' Overlap uORF GN=CSNK2A3 5' Overlap uORF E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep4_Slot2-5_1_3522 10mio-rep3 E_20221201_NO30_400nL_HLAc1_1e7_directIP_titration_rep3_Slot2-3_1_3514.9558.1421.2 2 2 1 10.57 6.75 5.76 3.81 68.0 6 1 1 6180.0 M52m Hydrogen 48 (N) 9 GEIKmTTSW (F) 9 TKDSVmVSW G E/I/K m\T\T|S\W 55.6 5 4 3 1 0 unbiased m:Oxidized methionine 33.026711 0.863234 534.7522 1052.508 15.9886 -5.9 0.39999999999999947 6.0 7035.9 nuORFdb_human ENST00000528848.2_1_11:11374530-11374710:- ENST00000528848.2_1_11:11374530-11374710:-|ENST00000609606.5_1_20:485838-489110:- CSNK2A3 5' Overlap uORF 5' Overlap uORF GN=CSNK2A3 5' Overlap uORF E_20221201_NO30_400nL_HLAc1_1e7_directIP_titration_rep3_Slot2-3_1_3514 20mio-rep1 E_20221201_NO30_400nL_HLAc1_2e7_directIP_titration_rep1_Slot2-4_1_3515.9362.1415.2 2 2 1 14.77 7.68 8.58 7.1 86.5 6 1 1 10300.0 M52m Hydrogen 48 (N) 9 GEIKmTTSW (F) 9 SRWmVTSW G E/I|K m\T|T|S\W 55.6 5 5 4 3 0 dual b/y bias m:Oxidized methionine 32.33889 0.866685 534.7515 1052.508 15.9866 -7.8 -1.5999999999999996 6.0 7035.9 nuORFdb_human ENST00000528848.2_1_11:11374530-11374710:- ENST00000528848.2_1_11:11374530-11374710:-|ENST00000609606.5_1_20:485838-489110:- CSNK2A3 5' Overlap uORF 5' Overlap uORF GN=CSNK2A3 5' Overlap uORF E_20221201_NO30_400nL_HLAc1_2e7_directIP_titration_rep1_Slot2-4_1_3515 20mio-rep2 E_20221201_NO30_400nL_HLAc1_2e7_directIP_titration_rep3_Slot2-4_1_3518.9669.1413.2 2 3 1 9.38 5.3 4.94 4.08 70.9 6 1 1 20900.0 M52m Hydrogen 48 (N) 9 GEIKmTTSW (F) 9 RTESTTTSW G E/I|K m\T\T|S\W 55.6 5 5 3 2 0 unbiased m:Oxidized methionine 33.400463 0.8679 534.751 1052.508 15.9866 -7.8 -1.5 6.0 7035.9 nuORFdb_human ENST00000528848.2_1_11:11374530-11374710:- ENST00000528848.2_1_11:11374530-11374710:-|ENST00000609606.5_1_20:485838-489110:- CSNK2A3 5' Overlap uORF 5' Overlap uORF GN=CSNK2A3 5' Overlap uORF E_20221201_NO30_400nL_HLAc1_2e7_directIP_titration_rep3_Slot2-4_1_3518 20mio-rep3 E_20221201_NO30_400nL_HLAc1_2e7_directIP_titration_rep4_Slot2-4_1_3519.9532.1408.2 2 1 1 11.45 4.01 6.34 7.44 72.3 7 1 1 4760.0 M52m Hydrogen 48 (N) 9 GEIKmTTSW (F) 9 RTESTTTSW G E/I/K|m\T|T|S\W 77.8 7 5 5 3 0 dual b/y bias m:Oxidized methionine 32.928993 0.871241 534.7525 1052.508 15.9906 -4.0 1.7999999999999998 6.0 7035.9 nuORFdb_human ENST00000528848.2_1_11:11374530-11374710:- ENST00000528848.2_1_11:11374530-11374710:-|ENST00000609606.5_1_20:485838-489110:- CSNK2A3 5' Overlap uORF 5' Overlap uORF GN=CSNK2A3 5' Overlap uORF E_20221201_NO30_400nL_HLAc1_2e7_directIP_titration_rep4_Slot2-4_1_3519 40mio-rep1 E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep1_Slot1-04_1_3496.9802.1380.2 2 2 1 9.29 4.41 4.87 4.87 72.2 6 1 1 25700.0 M52m Hydrogen 48 (N) 9 GEIKmTTSW (F) 9 QYTDDKIW G E/I/K m\T|T|S\W 55.6 5 4 4 2 0 unbiased m:Oxidized methionine 33.862926 0.88898 534.7551 1052.508 15.9946 -0.3 2.9000000000000004 6.0 7035.9 nuORFdb_human ENST00000528848.2_1_11:11374530-11374710:- ENST00000528848.2_1_11:11374530-11374710:-|ENST00000609606.5_1_20:485838-489110:- CSNK2A3 5' Overlap uORF 5' Overlap uORF GN=CSNK2A3 5' Overlap uORF E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep1_Slot1-04_1_3496 40mio-rep2 E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep2_Slot2-5_1_3520.9368.1408.2 2 2 1 14.84 8.17 8.92 6.66 86.0 7 1 1 19700.0 M52m Hydrogen 48 (N) 9 GEIKmTTSW (F) 9 TKDSVmVSW G/E/I/K m\T|T|S\W 77.8 7 4 5 2 0 unbiased m:Oxidized methionine 32.363598 0.871592 534.7517 1052.508 15.9886 -5.9 -1.0 6.0 7035.9 nuORFdb_human ENST00000528848.2_1_11:11374530-11374710:- ENST00000528848.2_1_11:11374530-11374710:-|ENST00000609606.5_1_20:485838-489110:- CSNK2A3 5' Overlap uORF 5' Overlap uORF GN=CSNK2A3 5' Overlap uORF E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep2_Slot2-5_1_3520 40mio-rep3 E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep3_Slot2-5_1_3521.9370.1409.2 2 2 1 17.55 8.81 10.74 8.74 90.0 7 1 1 17900.0 M52m Hydrogen 48 (N) 9 GEIKmTTSW (F) 9 TKDSVmVSW G E/I|K|m\T|T|S\W 77.8 7 6 5 4 0 dual b/y bias m:Oxidized methionine 32.372295 0.870977 534.7535 1052.508 15.9926 -2.2 2.3999999999999995 6.0 7035.9 nuORFdb_human ENST00000528848.2_1_11:11374530-11374710:- ENST00000528848.2_1_11:11374530-11374710:-|ENST00000609606.5_1_20:485838-489110:- CSNK2A3 5' Overlap uORF 5' Overlap uORF GN=CSNK2A3 5' Overlap uORF E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep3_Slot2-5_1_3521 40mio-rep4 E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep4_Slot2-5_1_3522.9379.1404.2 2 2 1 14.4 6.5 8.35 7.91 83.0 7 1 1 15300.0 M52m Hydrogen 48 (N) 9 GEIKmTTSW (F) 9 TKDSVmVSW G E/I|K/m/T|T|S\W 77.8 7 4 6 3 0 dual b/y bias m:Oxidized methionine 32.39278 0.873557 534.7534 1052.508 15.9906 -4.0 0.7000000000000002 6.0 7035.9 nuORFdb_human ENST00000528848.2_1_11:11374530-11374710:- ENST00000528848.2_1_11:11374530-11374710:-|ENST00000609606.5_1_20:485838-489110:- CSNK2A3 5' Overlap uORF 5' Overlap uORF GN=CSNK2A3 5' Overlap uORF E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep4_Slot2-5_1_3522 20mio-rep1 E_20221201_NO30_400nL_HLAc1_2e7_directIP_titration_rep1_Slot2-4_1_3515.12089.1483.2 2 1 1 10.08 6.34 2.41 3.74 71.1 7 1 1 7160.0 Hydrogen 28 (R) 9 GELPLKQSY (-) 9 GELPLPAGPPS G E/L|P/L/K/Q\S\Y 77.8 7 3 5 1 0 unbiased 41.756222 0.824084 517.7755 1034.552 -0.007 -6.7 -0.5 6.0 4088.8 nuORFdb_human ENST00000084798.8_1_19:49141296-49142147:- ENST00000084798.8_1_19:49141296-49142147:- CA11 3' Overlap dORF 3' Overlap dORF GN=CA11 3' Overlap dORF E_20221201_NO30_400nL_HLAc1_2e7_directIP_titration_rep1_Slot2-4_1_3515 40mio-rep1 E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep1_Slot1-04_1_3496.12390.1443.2 2 1 1 9.28 6.01 2.36 3.26 58.8 6 1 1 16200.0 Hydrogen 28 (R) 9 GELPLKQSY (-) 9 DAITLNPKY G E/L|P/L/K\Q S\Y 55.6 5 3 4 1 0 unbiased 42.799213 0.849354 517.7773 1034.552 -0.005 -4.8 -1.5999999999999996 6.0 4088.8 nuORFdb_human ENST00000084798.8_1_19:49141296-49142147:- ENST00000084798.8_1_19:49141296-49142147:- CA11 3' Overlap dORF 3' Overlap dORF GN=CA11 3' Overlap dORF E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep1_Slot1-04_1_3496 40mio-rep3 E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep3_Slot2-5_1_3521.12083.1464.2 2 1 1 11.59 6.02 2.43 5.57 75.0 7 1 1 10800.0 Hydrogen 28 (R) 9 GELPLKQSY (-) 9 GELPLPAGPPS G E/L|P/L/K/Q|S\Y 77.8 7 3 6 2 0 y-ion bias 41.742741 0.836221 517.776 1034.552 -0.007 -6.7 -2.1000000000000005 6.0 4088.8 nuORFdb_human ENST00000084798.8_1_19:49141296-49142147:- ENST00000084798.8_1_19:49141296-49142147:- CA11 3' Overlap dORF 3' Overlap dORF GN=CA11 3' Overlap dORF E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep3_Slot2-5_1_3521 40mio-rep4 E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep4_Slot2-5_1_3522.12082.1466.2 2 1 1 10.76 7.47 1.15 3.29 72.5 6 1 1 14000.0 Hydrogen 28 (R) 9 GELPLKQSY (-) 9 nLAPSLQKY G E/L|P/L/K Q\S\Y 55.6 5 3 4 1 0 unbiased 41.723858 0.834867 517.7759 1034.552 -0.007 -6.7 -2.0 6.0 4088.8 nuORFdb_human ENST00000084798.8_1_19:49141296-49142147:- ENST00000084798.8_1_19:49141296-49142147:- CA11 3' Overlap dORF 3' Overlap dORF GN=CA11 3' Overlap dORF E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep4_Slot2-5_1_3522 40mio-rep3 E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep3_Slot2-5_1_3521.11439.1472.2 2 1 1 11.05 5.4 5.69 5.65 73.6 7 1 1 7610.0 Hydrogen 46 (R) 9 GENGAILRY (G) 9 ENNALLRY G E/N/G/A|I|L/R\Y 77.8 7 3 6 2 0 y-ion bias 39.518806 0.831125 496.7596 992.516 -0.0032 -3.3 1.2999999999999998 6.0 10692.1 nuORFdb_human ENST00000375895.6_1_6:31632636-31633491:- ENST00000375895.6_1_6:31632636-31633491:- GPANK1 5' uORF 5' uORF GN=GPANK1 5' uORF E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep3_Slot2-5_1_3521 40mio-rep1 E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep1_Slot1-04_1_3496.27006.412.1 1 1 1 9.46 -0.77 10.27 10.23 76.8 6 0 0 3320.0 Hydrogen 23 (R) 9 GESAWGQFF (S) 9 MSSGGPAQFF G E S/A/W|G/Q\F\F 66.7 6 3 4 1 0 unbiased 93.295517 1.478014 1028.4403 1028.447 -0.0072 -7.0 -3.8 4.0 6802.5 nuORFdb_human ENST00000577066.2_1_17:79006875-79007058:- ENST00000577066.2_1_17:79006875-79007058:- BAIAP2-AS1 lincRNA lncRNA GN=BAIAP2-AS1 lincRNA E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep1_Slot1-04_1_3496 10mio-rep3 E_20221201_NO30_400nL_HLAc1_1e7_directIP_titration_rep3_Slot2-3_1_3514.17027.604.1 1 1 1 9.07 4.26 4.04 4.81 76.8 6 0 0 2590.0 Hydrogen 40 (G) 9 GESDSGLAW (F) 9 ESSGVDAAW G E/S D/S/G\L\A\W 55.6 5 3 3 0 0 unbiased 58.817257 1.363663 921.3893 921.395 -0.0059 -6.4 -0.10000000000000053 3.66 5494.1 nuORFdb_human T356158_9:17018428-17033578:+ T356158_9:17018428-17033578:+ lincRNA lncRNA GN=nan lincRNA E_20221201_NO30_400nL_HLAc1_1e7_directIP_titration_rep3_Slot2-3_1_3514 20mio-rep1 E_20221201_NO30_400nL_HLAc1_2e7_directIP_titration_rep1_Slot2-4_1_3515.17002.605.1 1 1 1 9.61 6.93 2.81 2.69 79.2 6 0 0 3580.0 Hydrogen 40 (G) 9 GESDSGLAW (F) 9 EGSEVGSAGE G E/S D/S/G|L\A\W 55.6 5 3 4 1 0 unbiased 58.717083 1.363219 921.389 921.395 -0.0059 -6.4 -0.20000000000000018 3.66 5494.1 nuORFdb_human T356158_9:17018428-17033578:+ T356158_9:17018428-17033578:+ lincRNA lncRNA GN=nan lincRNA E_20221201_NO30_400nL_HLAc1_2e7_directIP_titration_rep1_Slot2-4_1_3515 40mio-rep1 E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep1_Slot1-04_1_3496.17314.578.1 1 2 1 10.64 6.67 3.61 3.98 81.3 6 0 0 21400.0 Hydrogen 40 (G) 9 GESDSGLAW (F) 9 LnQSnSAW G E/S D\S|G\L\A\W 55.6 5 5 2 1 0 b-ion bias 59.807137 1.379279 921.3894 921.395 -0.0059 -6.4 -3.2 3.66 5494.1 nuORFdb_human T356158_9:17018428-17033578:+ T356158_9:17018428-17033578:+ lincRNA lncRNA GN=nan lincRNA E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep1_Slot1-04_1_3496 40mio-rep2 E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep2_Slot2-5_1_3520.16958.593.1 1 1 1 11.09 6.08 4.69 5.01 81.1 6 0 0 5790.0 Hydrogen 40 (G) 9 GESDSGLAW (F) 9 GEGSSDLQE G E/S D|S\G\L|A\W 55.6 5 5 3 2 0 unbiased 58.578777 1.370273 921.3886 921.395 -0.0059 -6.4 -1.5 3.66 5494.1 nuORFdb_human T356158_9:17018428-17033578:+ T356158_9:17018428-17033578:+ lincRNA lncRNA GN=nan lincRNA E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep2_Slot2-5_1_3520 10mio-rep2 E_20221201_NO30_400nL_HLAc1_1e7_directIP_titration_rep2_Slot2-3_1_3513.8722.1377.2 2 2 1 10.65 4.98 5.67 5.67 68.9 7 1 1 5080.0 Hydrogen 73 (L) 10 GETELGHARW (R) 10 DVLNSPLEGPD G E/T|E|L|G-H/A-R\W 80.0 8 4 5 3 2 unbiased 30.135302 0.890605 578.2758 1155.554 -0.0094 -8.2 -1.4999999999999991 5.4 13836.3 nuORFdb_human ENST00000539387.5_1_4:140216240-140223649:- ENST00000539387.5_1_4:140216240-140223649:- NDUFC1 5' Overlap uORF 5' Overlap uORF GN=NDUFC1 5' Overlap uORF E_20221201_NO30_400nL_HLAc1_1e7_directIP_titration_rep2_Slot2-3_1_3513 20mio-rep1 E_20221201_NO30_400nL_HLAc1_2e7_directIP_titration_rep1_Slot2-4_1_3515.8631.1379.2 2 2 1 10.12 4.45 3.09 5.67 73.8 7 1 1 13400.0 Hydrogen 73 (L) 10 GETELGHARW (R) 10 EGSAPALImNH G/E/T/E/L|G-H|A R\W 80.0 8 3 6 2 1 y-ion bias 29.815519 0.889527 578.2778 1155.554 -0.0054 -4.7 1.5 5.4 13836.3 nuORFdb_human ENST00000539387.5_1_4:140216240-140223649:- ENST00000539387.5_1_4:140216240-140223649:- NDUFC1 5' Overlap uORF 5' Overlap uORF GN=NDUFC1 5' Overlap uORF E_20221201_NO30_400nL_HLAc1_2e7_directIP_titration_rep1_Slot2-4_1_3515 20mio-rep2 E_20221201_NO30_400nL_HLAc1_2e7_directIP_titration_rep3_Slot2-4_1_3518.8821.1369.2 2 2 1 12.71 7.13 5.75 5.59 75.0 6 1 1 11600.0 Hydrogen 73 (L) 10 GETELGHARW (R) 10 EASEIPLGPQn G E/T|E|L|G\H\A R W 50.0 5 5 4 3 0 unbiased 30.472404 0.895494 578.2769 1155.554 -0.0074 -6.4 -0.10000000000000053 5.4 13836.3 nuORFdb_human ENST00000539387.5_1_4:140216240-140223649:- ENST00000539387.5_1_4:140216240-140223649:- NDUFC1 5' Overlap uORF 5' Overlap uORF GN=NDUFC1 5' Overlap uORF E_20221201_NO30_400nL_HLAc1_2e7_directIP_titration_rep3_Slot2-4_1_3518 20mio-rep3 E_20221201_NO30_400nL_HLAc1_2e7_directIP_titration_rep4_Slot2-4_1_3519.8664.1598.3 3 3 1 11.31 4.69 5.65 6.61 69.3 8 1 1 15600.0 Hydrogen 73 (L) 10 GETELGHARW (R) 10 nPRFGSYKEG G/E/T/E/L/G/H/A R\W 80.0 8 1 7 0 0 y-ion bias 29.929787 0.751706 385.8555 1155.554 -0.0037 -3.2 2.5999999999999996 5.4 13836.3 nuORFdb_human ENST00000539387.5_1_4:140216240-140223649:- ENST00000539387.5_1_4:140216240-140223649:- NDUFC1 5' Overlap uORF 5' Overlap uORF GN=NDUFC1 5' Overlap uORF E_20221201_NO30_400nL_HLAc1_2e7_directIP_titration_rep4_Slot2-4_1_3519 40mio-rep1 E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep1_Slot1-04_1_3496.8924.1348.2 2 2 1 15.68 6.46 8.73 9.22 87.7 8 1 1 22000.0 Hydrogen 73 (L) 10 GETELGHARW (R) 10 EGSAPALImNH G/E/T/E|L|G\H|A R\W 80.0 8 5 6 3 0 unbiased 30.830988 0.908917 578.279 1155.554 -0.0034 -3.0 0.20000000000000018 5.4 13836.3 nuORFdb_human ENST00000539387.5_1_4:140216240-140223649:- ENST00000539387.5_1_4:140216240-140223649:- NDUFC1 5' Overlap uORF 5' Overlap uORF GN=NDUFC1 5' Overlap uORF E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep1_Slot1-04_1_3496 40mio-rep2 E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep2_Slot2-5_1_3520.8627.1366.2 2 2 1 17.33 7.56 7.52 9.77 87.1 8 1 1 25600.0 Hydrogen 73 (L) 10 GETELGHARW (R) 10 DVLNSPLEGPD G E/T/E|L|G\H|A/R\W 80.0 8 5 6 3 0 unbiased 29.804853 0.897474 578.2775 1155.554 -0.0074 -6.4 -1.5 5.4 13836.3 nuORFdb_human ENST00000539387.5_1_4:140216240-140223649:- ENST00000539387.5_1_4:140216240-140223649:- NDUFC1 5' Overlap uORF 5' Overlap uORF GN=NDUFC1 5' Overlap uORF E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep2_Slot2-5_1_3520 40mio-rep3 E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep3_Slot2-5_1_3521.8649.1365.2 2 2 1 15.65 5.23 9.43 10.42 84.6 8 1 1 24100.0 Hydrogen 73 (L) 10 GETELGHARW (R) 10 DVLNSPLEGPD G E/T|E|L/G\H|A|R\W 80.0 8 6 6 4 0 dual b/y bias 29.879637 0.898189 578.2784 1155.554 -0.0054 -4.7 -0.10000000000000053 5.4 13836.3 nuORFdb_human ENST00000539387.5_1_4:140216240-140223649:- ENST00000539387.5_1_4:140216240-140223649:- NDUFC1 5' Overlap uORF 5' Overlap uORF GN=NDUFC1 5' Overlap uORF E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep3_Slot2-5_1_3521 40mio-rep4 E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep4_Slot2-5_1_3522.8649.1364.2 2 2 1 15.11 5.32 7.98 9.78 83.1 8 1 1 20300.0 Hydrogen 73 (L) 10 GETELGHARW (R) 10 DVLNSPLEGPD G E/T/E|L|G\H|A|R\W 80.0 8 6 6 4 0 dual b/y bias 29.872982 0.899191 578.2779 1155.554 -0.0054 -4.7 0.0 5.4 13836.3 nuORFdb_human ENST00000539387.5_1_4:140216240-140223649:- ENST00000539387.5_1_4:140216240-140223649:- NDUFC1 5' Overlap uORF 5' Overlap uORF GN=NDUFC1 5' Overlap uORF E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep4_Slot2-5_1_3522 5mio-rep1 E_20221201_NO30_400nL_HLAc1_5e6_directIP_titration_rep1_Slot1-37_1_3502.8972.1370.2 2 1 1 9.16 4.51 5.66 4.65 63.5 7 1 1 3480.0 Hydrogen 73 (L) 10 GETELGHARW (R) 10 EGSAPALImNH G/E/T/E|L|G-H/A R\W 80.0 8 3 6 2 1 y-ion bias 30.996529 0.895207 578.2783 1155.554 -0.0054 -4.7 2.3 5.4 13836.3 nuORFdb_human ENST00000539387.5_1_4:140216240-140223649:- ENST00000539387.5_1_4:140216240-140223649:- NDUFC1 5' Overlap uORF 5' Overlap uORF GN=NDUFC1 5' Overlap uORF E_20221201_NO30_400nL_HLAc1_5e6_directIP_titration_rep1_Slot1-37_1_3502 10mio-rep1 E_20221201_NO30_400nL_HLAc1_1e7_directIP_titration_rep1_Slot2-3_1_3512.25191.1413.2 2 1 1 8.24 3.36 4.02 4.87 64.3 6 0 0 1580.0 C67c Hydrogen 60 (Q) 9 GETTLLVcF (V) 9 LTLEGTVFc G E/T\T/L/L|V|c F 55.6 5 3 5 2 0 unbiased c:Cysteinylation 87.012505 0.867939 551.2481 1039.513 61.9759 -6.1 1.6000000000000005 4.0 9116.3 smORF_v4|nuORFdb_human NUP50-AS1_22:45530583-45559517:- NUP50-AS1_22:45530583-45559517:-|ENST00000609284.1_1_22:45530585-45548378:-|ENST00000426282.6_1_22:45530585-45559517:- NUP50-AS1 lncRNA 22:45530583-45559517:- 22 NUP50-AS1 lincRNA lncRNA GN=NUP50-AS1 E_20221201_NO30_400nL_HLAc1_1e7_directIP_titration_rep1_Slot2-3_1_3512 10mio-rep2 E_20221201_NO30_400nL_HLAc1_1e7_directIP_titration_rep2_Slot2-3_1_3513.25114.1411.2 2 1 1 9.03 2.48 4.93 6.55 70.6 7 0 0 1460.0 C67c Hydrogen 60 (Q) 9 GETTLLVcF (V) 9 SLDVSIVFc G E/T/T/L|L/V/c-F 77.8 7 1 6 1 1 y-ion bias c:Cysteinylation 86.739372 0.86928 551.2448 1039.513 61.9699 -11.6 -4.8999999999999995 4.0 9116.3 smORF_v4|nuORFdb_human NUP50-AS1_22:45530583-45559517:- NUP50-AS1_22:45530583-45559517:-|ENST00000609284.1_1_22:45530585-45548378:-|ENST00000426282.6_1_22:45530585-45559517:- NUP50-AS1 lncRNA 22:45530583-45559517:- 22 NUP50-AS1 lincRNA lncRNA GN=NUP50-AS1 E_20221201_NO30_400nL_HLAc1_1e7_directIP_titration_rep2_Slot2-3_1_3513 20mio-rep1 E_20221201_NO30_400nL_HLAc1_2e7_directIP_titration_rep1_Slot2-4_1_3515.25086.1408.2 2 1 1 11.81 3.93 2.78 7.88 80.6 6 0 0 4680.0 C67c Hydrogen 60 (Q) 9 GETTLLVcF (V) 9 SLDVSIVFc G E/T/T|L|L|V/c F 55.6 5 3 6 3 0 dual b/y bias c:Cysteinylation 86.627464 0.871129 551.2485 1039.513 61.9759 -6.1 0.10000000000000053 4.0 9116.3 smORF_v4|nuORFdb_human NUP50-AS1_22:45530583-45559517:- NUP50-AS1_22:45530583-45559517:-|ENST00000609284.1_1_22:45530585-45548378:-|ENST00000426282.6_1_22:45530585-45559517:- NUP50-AS1 lncRNA 22:45530583-45559517:- 22 NUP50-AS1 lincRNA lncRNA GN=NUP50-AS1 E_20221201_NO30_400nL_HLAc1_2e7_directIP_titration_rep1_Slot2-4_1_3515 20mio-rep2 E_20221201_NO30_400nL_HLAc1_2e7_directIP_titration_rep3_Slot2-4_1_3518.25125.1400.2 2 1 1 7.4 4.24 7.4 64.0 7 0 0 2540.0 C67c Hydrogen 60 (Q) 9 GETTLLVcF (V) 9 G E/T/T/L/L|V/c-F 77.8 7 1 6 1 1 y-ion bias c:Cysteinylation 86.780792 0.876311 551.2468 1039.513 61.9739 -8.0 -1.7000000000000002 4.0 9116.3 smORF_v4|nuORFdb_human NUP50-AS1_22:45530583-45559517:- NUP50-AS1_22:45530583-45559517:-|ENST00000609284.1_1_22:45530585-45548378:-|ENST00000426282.6_1_22:45530585-45559517:- NUP50-AS1 lncRNA 22:45530583-45559517:- 22 NUP50-AS1 lincRNA lncRNA GN=NUP50-AS1 E_20221201_NO30_400nL_HLAc1_2e7_directIP_titration_rep3_Slot2-4_1_3518 40mio-rep1 E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep1_Slot1-04_1_3496.25334.1378.2 2 2 1 12.23 2.78 3.8 9.45 81.5 6 0 0 17500.0 C67c Hydrogen 60 (Q) 9 GETTLLVcF (V) 9 SLDVSIVFc G E/T|T|L|L|V/c F 55.6 5 4 6 4 0 dual b/y bias c:Cysteinylation 87.519142 0.889857 551.2478 1039.513 61.9759 -6.1 -2.8999999999999995 4.0 9116.3 smORF_v4|nuORFdb_human NUP50-AS1_22:45530583-45559517:- NUP50-AS1_22:45530583-45559517:-|ENST00000609284.1_1_22:45530585-45548378:-|ENST00000426282.6_1_22:45530585-45559517:- NUP50-AS1 lncRNA 22:45530583-45559517:- 22 NUP50-AS1 lincRNA lncRNA GN=NUP50-AS1 E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep1_Slot1-04_1_3496 40mio-rep2 E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep2_Slot2-5_1_3520.25105.1393.2 2 1 1 12.02 2.28 3.63 9.73 77.9 7 0 0 10500.0 C67c Hydrogen 60 (Q) 9 GETTLLVcF (V) 9 SLDVSIVFc G E/T|T/L|L|V/c-F 77.8 7 3 6 3 1 dual b/y bias c:Cysteinylation 86.724213 0.880434 551.2488 1039.513 61.9779 -4.3 0.6000000000000005 4.0 9116.3 smORF_v4|nuORFdb_human NUP50-AS1_22:45530583-45559517:- NUP50-AS1_22:45530583-45559517:-|ENST00000609284.1_1_22:45530585-45548378:-|ENST00000426282.6_1_22:45530585-45559517:- NUP50-AS1 lncRNA 22:45530583-45559517:- 22 NUP50-AS1 lincRNA lncRNA GN=NUP50-AS1 E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep2_Slot2-5_1_3520 40mio-rep3 E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep3_Slot2-5_1_3521.25117.1401.2 2 1 1 8.85 1.88 3.49 6.97 66.1 6 0 0 7050.0 C67c Hydrogen 60 (Q) 9 GETTLLVcF (V) 9 SLDVSIVFc G E/T/T/L|L|V/c F 55.6 5 2 6 2 0 y-ion bias c:Cysteinylation 86.763771 0.875576 551.2479 1039.513 61.9759 -6.1 -1.5 4.0 9116.3 smORF_v4|nuORFdb_human NUP50-AS1_22:45530583-45559517:- NUP50-AS1_22:45530583-45559517:-|ENST00000609284.1_1_22:45530585-45548378:-|ENST00000426282.6_1_22:45530585-45559517:- NUP50-AS1 lncRNA 22:45530583-45559517:- 22 NUP50-AS1 lincRNA lncRNA GN=NUP50-AS1 E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep3_Slot2-5_1_3521 40mio-rep1 E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep1_Slot1-04_1_3496.13286.1420.2 2 2 1 13.38 7.45 2.93 5.93 88.2 7 1 1 16500.0 Hydrogen 9 (M) 9 GEWESERGW (A) 9 nMAPSSRWDA G E/W|E|S\E/R|G\W 77.8 7 5 5 3 0 dual b/y bias 45.894299 0.863903 568.2428 1135.48 -0.0016 -1.4 1.8000000000000003 4.25 2824.0 nuORFdb_human ENST00000527985.5_1_11:57435324-57435402:+ ENST00000527985.5_1_11:57435324-57435402:+ ZDHHC5 5' uORF 5' uORF GN=ZDHHC5 5' uORF E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep1_Slot1-04_1_3496 40mio-rep2 E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep2_Slot2-5_1_3520.12954.1438.2 2 1 1 17.24 9.33 4.18 7.92 93.6 8 1 1 9410.0 Hydrogen 9 (M) 9 GEWESERGW (A) 9 GEWGVSEWEG G/E/W|E|S\E|R|G\W 100.0 9 6 6 4 0 dual b/y bias 44.749401 0.85257 568.2418 1135.48 -0.0036 -3.2 1.7000000000000002 4.25 2824.0 nuORFdb_human ENST00000527985.5_1_11:57435324-57435402:+ ENST00000527985.5_1_11:57435324-57435402:+ ZDHHC5 5' uORF 5' uORF GN=ZDHHC5 5' uORF E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep2_Slot2-5_1_3520 40mio-rep3 E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep3_Slot2-5_1_3521.12963.1444.2 2 1 1 13.88 8.71 3.14 5.17 93.7 6 1 1 10700.0 Hydrogen 9 (M) 9 GEWESERGW (A) 9 GEEWGICVGE G E/W|E|S E/R|G\W 55.6 5 4 5 3 0 dual b/y bias 44.784805 0.848758 568.2414 1135.48 -0.0056 -4.9 -0.3000000000000007 4.25 2824.0 nuORFdb_human ENST00000527985.5_1_11:57435324-57435402:+ ENST00000527985.5_1_11:57435324-57435402:+ ZDHHC5 5' uORF 5' uORF GN=ZDHHC5 5' uORF E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep3_Slot2-5_1_3521 40mio-rep4 E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep4_Slot2-5_1_3522.12954.1442.2 2 1 1 13.32 7.06 3.06 6.26 88.7 7 1 1 11500.0 Hydrogen 9 (M) 9 GEWESERGW (A) 9 GEWGVSEWEG G E/W|E|S\E/R|G\W 77.8 7 5 5 3 0 dual b/y bias 44.7342 0.85005 568.2414 1135.48 -0.0056 -4.9 -0.20000000000000018 4.25 2824.0 nuORFdb_human ENST00000527985.5_1_11:57435324-57435402:+ ENST00000527985.5_1_11:57435324-57435402:+ ZDHHC5 5' uORF 5' uORF GN=ZDHHC5 5' uORF E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep4_Slot2-5_1_3522 40mio-rep2 E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep2_Slot2-5_1_3520.14562.1320.2 2 1 1 9.6 0.94 4.29 8.65 68.5 6 0 0 7240.0 Hydrogen 29 (K) 11 GEWHPGEPYGY (R) 11 ANTDPCAPPFDS G E/W H|P G E|P|Y\G\Y 36.4 4 5 4 3 0 unbiased 50.303425 0.926376 646.2669 1291.538 -0.0111 -8.6 -3.6999999999999993 4.51 14209.7 nuORFdb_human ENST00000318100.8_1_20:62289730-62293901:+ ENST00000318100.8_1_20:62289730-62293901:+|ENST00000488316.1_1_20:62290811-62293163:+|ENST00000356810.5_1_20:62290811-62293793:+|ENST00000370018.7_1_20:62290811-62293901:+|ENST00000508582.6_1_20:62290811-62293901:+ RTEL1 5' Overlap uORF 5' Overlap uORF GN=RTEL1 5' Overlap uORF E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep2_Slot2-5_1_3520 40mio-rep3 E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep3_Slot2-5_1_3521.14564.1326.2 2 2 1 10.07 7.26 10.07 57.2 7 0 0 12600.0 Hydrogen 29 (K) 11 GEWHPGEPYGY (R) 11 G E/W/H|P G E|P/Y|G\Y 54.5 6 4 6 3 0 unbiased 50.312515 0.922421 646.2676 1291.538 -0.0091 -7.0 -2.4000000000000004 4.51 14209.7 nuORFdb_human ENST00000318100.8_1_20:62289730-62293901:+ ENST00000318100.8_1_20:62289730-62293901:+|ENST00000488316.1_1_20:62290811-62293163:+|ENST00000356810.5_1_20:62290811-62293793:+|ENST00000370018.7_1_20:62290811-62293901:+|ENST00000508582.6_1_20:62290811-62293901:+ RTEL1 5' Overlap uORF 5' Overlap uORF GN=RTEL1 5' Overlap uORF E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep3_Slot2-5_1_3521 40mio-rep4 E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep4_Slot2-5_1_3522.14558.1323.2 2 1 1 9.7 2.19 3.64 7.5 63.4 6 0 0 6440.0 Hydrogen 29 (K) 11 GEWHPGEPYGY (R) 11 TSEAQVKScGY G E/W H|P G E|P/Y|G\Y 36.4 4 4 5 3 0 unbiased 50.277851 0.92434 646.2693 1291.538 -0.0071 -5.5 -0.7999999999999998 4.51 14209.7 nuORFdb_human ENST00000318100.8_1_20:62289730-62293901:+ ENST00000318100.8_1_20:62289730-62293901:+|ENST00000488316.1_1_20:62290811-62293163:+|ENST00000356810.5_1_20:62290811-62293793:+|ENST00000370018.7_1_20:62290811-62293901:+|ENST00000508582.6_1_20:62290811-62293901:+ RTEL1 5' Overlap uORF 5' Overlap uORF GN=RTEL1 5' Overlap uORF E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep4_Slot2-5_1_3522 40mio-rep1 E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep1_Slot1-04_1_3496.27593.1328.2 2 1 1 10.38 6.89 4.05 3.49 74.9 6 1 1 4870.0 Hydrogen 14 (E) 9 GEWKDVWIW (M) 9 RnNELSWLW G E/W/K\D V|W|I\W 55.6 5 4 4 2 0 unbiased 95.326294 0.921317 609.8006 1218.594 0.0005 0.4 3.6 4.37 10033.2 nuORFdb_human ENST00000547963.1_1_12:114184084-114184339:- ENST00000547963.1_1_12:114184084-114184339:- LINC01234 lincRNA lncRNA GN=LINC01234 lincRNA E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep1_Slot1-04_1_3496 SCP E_20221201_NO30_400nL_HLAc1_4e7_PDAC_Slot2-6_1_3524.19909.1304.2 2 1 1 10.57 8.52 1.23 2.04 63.4 6 0 0 6510.0 Hydrogen 89 (D) 10 GFVLGTLSLW (N) 10 VSAIFSLGLGE G F/V/L G\T\L|S|L\W 60.0 6 5 4 2 0 unbiased 68.778183 0.936074 546.8068 1092.609 -0.0021 -1.9 0.5 5.52 14958.7 nuORFdb_human ENST00000353383.5_2_17:17713366-17713792:+ ENST00000353383.5_2_17:17713366-17713792:+ RAI1 3' dORF 3' dORF GN=RAI1 3' dORF E_20221201_NO30_400nL_HLAc1_4e7_PDAC_Slot2-6_1_3524 SCP E_20221201_NO30_400nL_HLAc1_4e7_PDAC_Slot2-6_1_3524.28065.1435.2 2 1 1 11.04 8.44 0.66 2.6 81.1 5 0 0 27800.0 Hydrogen 67 (S) 9 GHISLILHL (N) 9 KELHLIHI G H I S|L\I|L|H\L 55.6 5 5 3 3 0 dual b/y bias 96.959915 0.854573 501.807 1002.61 -0.0028 -2.7 -0.30000000000000027 6.92 10849.5 nuORFdb_human ENST00000478282.1_1_21:44513855-44514134:- ENST00000478282.1_1_21:44513855-44514134:- U2AF1 ncRNA Retained Intron lncRNA GN=U2AF1 ncRNA Retained Intron E_20221201_NO30_400nL_HLAc1_4e7_PDAC_Slot2-6_1_3524 40mio-rep1 E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep1_Slot1-04_1_3496.22165.1419.2 2 1 1 8.93 3.69 4.34 5.24 70.3 5 1 1 6460.0 Hydrogen 14 (G) 9 GINTKTFLW (K) 9 LGISGPVPPGW G I/N/T/K T F|L\W 44.4 4 2 4 1 0 y-ion bias 76.568672 0.864177 540.2977 1079.588 0.0003 0.3 3.5 8.75 4328.9 nuORFdb_human ENST00000509479.6_1_4:141074963-141075077:- ENST00000509479.6_1_4:141074963-141075077:- MAML3 5' uORF 5' uORF GN=MAML3 5' uORF E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep1_Slot1-04_1_3496 40mio-rep2 E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep2_Slot2-5_1_3520.21979.1439.2 2 1 1 10.77 5.49 5.31 5.28 72.6 6 1 1 4640.0 Hydrogen 14 (G) 9 GINTKTFLW (K) 9 GFSRGVSLSGL G I/N/T|K T/F|L\W 55.6 5 3 5 2 0 unbiased 75.92749 0.851598 540.2936 1079.588 -0.0077 -7.1 -2.1999999999999993 8.75 4328.9 nuORFdb_human ENST00000509479.6_1_4:141074963-141075077:- ENST00000509479.6_1_4:141074963-141075077:- MAML3 5' uORF 5' uORF GN=MAML3 5' uORF E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep2_Slot2-5_1_3520 Eva-50mio-3fr AL20210827_EV_HLA_PDAC_PANFRO413_2D_50e6_W632_classI_fxn2.29666.29766.2 2 1 1 12.87 7.26 5.75 5.61 84.7 5 0 0 87800000.0 Hydrogen 32 (V) 9 GLAEHLYLA (L) 9 YGIEHALAL G L\A E H\L\Y|L\A 44.4 4 5 1 1 0 b-ion bias 0.999 100.0 54.78 39.69 0.0 493.7689 986.531 -0.0001 -0.1 -0.4 5.24 4484.2 nuORFdb_human ENST00000479686.5_1_1:43830638-43833178:- ENST00000479686.5_1_1:43830638-43833178:-|ENST00000487209.5_1_1:43830638-43833598:- ELOVL1 5' Overlap uORF 5' Overlap uORF GN=ELOVL1 5' Overlap uORF AL20210827_EV_HLA_PDAC_PANFRO413_2D_50e6_W632_classI_fxn2 Exploris AL20221201_MKP_PDAC_01.39051.39304.2 2 1 1 11.75 6.81 4.32 4.93 79.9 5 0 0 451000000.0 Hydrogen 32 (V) 9 GLAEHLYLA (L) 9 YGIEHALAL G L|A E H\L\Y|L\A 44.4 4 5 2 2 0 b-ion bias 0.997 85.3 60.83 12.92 0.0 493.7694 986.531 0.0009 0.9 0.09999999999999998 5.24 4484.2 nuORFdb_human ENST00000479686.5_1_1:43830638-43833178:- ENST00000479686.5_1_1:43830638-43833178:-|ENST00000487209.5_1_1:43830638-43833598:- ELOVL1 5' Overlap uORF 5' Overlap uORF GN=ELOVL1 5' Overlap uORF AL20221201_MKP_PDAC_01 SCP E_20221201_NO30_400nL_HLAc1_4e7_PDAC_Slot2-6_1_3524.17778.329.1 1 4 1 16.84 8.81 9.67 8.03 96.2 7 0 0 106000.0 Hydrogen 32 (V) 9 GLAEHLYLA (L) 9 LQERGEALA G L/A|E|H\L\Y\L\A 77.8 7 6 3 2 0 b-ion bias 61.417274 1.527018 986.528 986.531 -0.0026 -2.6 -0.20000000000000018 5.24 4484.2 nuORFdb_human ENST00000479686.5_1_1:43830638-43833178:- ENST00000479686.5_1_1:43830638-43833178:-|ENST00000487209.5_1_1:43830638-43833598:- ELOVL1 5' Overlap uORF 5' Overlap uORF GN=ELOVL1 5' Overlap uORF E_20221201_NO30_400nL_HLAc1_4e7_PDAC_Slot2-6_1_3524 10mio-rep1 E_20221201_NO30_400nL_HLAc1_1e7_directIP_titration_rep1_Slot2-3_1_3512.11479.1527.2 2 1 1 7.86 6.47 1.9 1.39 90.2 6 1 1 4070.0 Hydrogen 5 (P) 9 GLARDAVPV (-) 9 VAATPSRVP G L/A/R D\A\V|P\V 55.6 5 4 3 1 0 unbiased 39.65934 0.7968 449.2577 897.515 -0.0065 -7.3 0.40000000000000036 5.84 1398.7 nuORFdb_human ENST00000512287.1_1_5:80531836-80531875:- ENST00000512287.1_1_5:80531836-80531875:- CKMT2-AS1 Antisense lncRNA GN=CKMT2-AS1 Antisense E_20221201_NO30_400nL_HLAc1_1e7_directIP_titration_rep1_Slot2-3_1_3512 10mio-rep2 E_20221201_NO30_400nL_HLAc1_1e7_directIP_titration_rep2_Slot2-3_1_3513.11450.1534.2 2 1 1 9.6 7.38 2.55 2.22 89.8 6 1 1 6480.0 Hydrogen 5 (P) 9 GLARDAVPV (-) 9 LGQRDLPV G L/A R\D\A\V|P\V 55.6 5 5 2 1 0 b-ion bias 39.558018 0.7923 449.2575 897.515 -0.0065 -7.3 -0.5999999999999996 5.84 1398.7 nuORFdb_human ENST00000512287.1_1_5:80531836-80531875:- ENST00000512287.1_1_5:80531836-80531875:- CKMT2-AS1 Antisense lncRNA GN=CKMT2-AS1 Antisense E_20221201_NO30_400nL_HLAc1_1e7_directIP_titration_rep2_Slot2-3_1_3513 20mio-rep1 E_20221201_NO30_400nL_HLAc1_2e7_directIP_titration_rep1_Slot2-4_1_3515.25026.465.1 1 1 1 10.09 8.36 0.97 1.72 74.2 6 0 0 3250.0 Hydrogen 72 (N) 9 GLDAIITQV (Q) 9 VQELITQV G L D\A\I|I|T\Q\V 66.7 6 6 2 2 0 b-ion bias 86.419693 1.446896 929.525 929.53 -0.0052 -5.6 0.6000000000000005 3.8 19157.9 nuORFdb_human ENST00000586749.1_1_19:649375-650400:- ENST00000586749.1_1_19:649375-650400:- RNF126 3' Overlap dORF 3' Overlap dORF GN=RNF126 3' Overlap dORF E_20221201_NO30_400nL_HLAc1_2e7_directIP_titration_rep1_Slot2-4_1_3515 40mio-rep3 E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep3_Slot2-5_1_3521.25018.453.1 1 1 1 9.66 7.94 2.17 1.72 70.1 7 0 0 5840.0 Hydrogen 72 (N) 9 GLDAIITQV (Q) 9 ITNLLEGAV G L/D\A\I|I\T\Q\V 77.8 7 6 2 1 0 b-ion bias 86.422081 1.45395 929.5235 929.53 -0.0062 -6.7 -2.1000000000000005 3.8 19157.9 nuORFdb_human ENST00000586749.1_1_19:649375-650400:- ENST00000586749.1_1_19:649375-650400:- RNF126 3' Overlap dORF 3' Overlap dORF GN=RNF126 3' Overlap dORF E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep3_Slot2-5_1_3521 20mio-rep1 E_20221201_NO30_400nL_HLAc1_2e7_directIP_titration_rep1_Slot2-4_1_3515.16996.1429.2 2 1 1 9.65 2.62 7.03 7.03 62.7 7 1 1 4830.0 M17m Hydrogen 13 (M) 9 GLFFmKQSV (-) 9 GIFSPGAGAGAAP G/L/F|F/m|K Q\S\V 77.8 7 4 5 2 0 unbiased m:Oxidized methionine 58.696388 0.858265 536.7743 1056.555 15.9861 -8.3 -2.1000000000000005 8.75 2454.0 nuORFdb_human ENST00000559345.5_1_15:79165548-79172860:+ ENST00000559345.5_1_15:79165548-79172860:+|ENST00000558746.5_1_15:79165393-79172860:+ MORF4L1 5' uORF 5' uORF GN=MORF4L1 5' uORF E_20221201_NO30_400nL_HLAc1_2e7_directIP_titration_rep1_Slot2-4_1_3515 20mio-rep3 E_20221201_NO30_400nL_HLAc1_2e7_directIP_titration_rep4_Slot2-4_1_3519.17044.1426.2 2 1 1 10.7 3.96 6.81 6.74 68.5 6 1 1 5750.0 M17m Hydrogen 13 (M) 9 GLFFmKQSV (-) 9 LGFFSmKLGG G L/F/F/m|K Q\S\V 55.6 5 3 4 1 0 unbiased m:Oxidized methionine 58.869778 0.85989 536.7758 1056.555 15.9901 -4.5 1.2999999999999998 8.75 2454.0 nuORFdb_human ENST00000559345.5_1_15:79165548-79172860:+ ENST00000559345.5_1_15:79165548-79172860:+|ENST00000558746.5_1_15:79165393-79172860:+ MORF4L1 5' uORF 5' uORF GN=MORF4L1 5' uORF E_20221201_NO30_400nL_HLAc1_2e7_directIP_titration_rep4_Slot2-4_1_3519 40mio-rep1 E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep1_Slot1-04_1_3496.17172.1399.2 2 1 1 12.59 7.3 6.22 5.29 76.3 5 1 1 7140.0 M17m Hydrogen 13 (M) 9 GLFFmKQSV (-) 9 VAFmLSKNF G L/F|F/m K Q\S\V 44.4 4 3 3 1 0 unbiased m:Oxidized methionine 59.316238 0.87684 536.7772 1056.555 15.9921 -2.7 0.5 8.75 2454.0 nuORFdb_human ENST00000559345.5_1_15:79165548-79172860:+ ENST00000559345.5_1_15:79165548-79172860:+|ENST00000558746.5_1_15:79165393-79172860:+ MORF4L1 5' uORF 5' uORF GN=MORF4L1 5' uORF E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep1_Slot1-04_1_3496 40mio-rep1 E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep1_Slot1-04_1_3496.28240.85.1 1 1 1 11.64 9.2 3.09 2.44 83.8 6 0 0 8370.0 Hydrogen 91 (V) 11 GLISSVEIYSL (-) 11 LKVTnELYTV G L I/S S V|E\I\Y\S\L 45.5 5 5 2 1 0 b-ion bias 97.561134 1.670165 1180.6404 1180.646 -0.006 -5.1 -1.8999999999999995 4.0 10308.9 nuORFdb_human ENST00000366600.7_1_1:235658098-235667703:- ENST00000366600.7_1_1:235658098-235667703:- B3GALNT2 5' Overlap uORF 5' Overlap uORF GN=B3GALNT2 5' Overlap uORF E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep1_Slot1-04_1_3496 20mio-rep1 E_20221201_NO30_400nL_HLAc1_2e7_directIP_titration_rep1_Slot2-4_1_3515.28474.379.1 1 1 1 9.21 5.24 3.18 3.98 66.6 5 0 0 3990.0 Hydrogen 5 (R) 9 GLLEEAWGL (-) 9 LQEELEGLG G L L E|E\A|W\G\L 55.6 5 5 2 2 0 b-ion bias 98.323738 1.497873 987.5095 987.515 -0.0056 -5.6 0.6000000000000005 3.8 1527.7 nuORFdb_human ENST00000209728.8_1_17:38444255-38444294:+ ENST00000209728.8_1_17:38444255-38444294:+ CDC6 5' uORF 5' uORF GN=CDC6 5' uORF E_20221201_NO30_400nL_HLAc1_2e7_directIP_titration_rep1_Slot2-4_1_3515 SCP E_20221201_NO30_400nL_HLAc1_4e7_PDAC_Slot2-6_1_3524.29175.357.1 1 1 1 7.63 4.43 2.03 3.2 66.7 5 0 0 33700.0 Hydrogen 5 (R) 9 GLLEEAWGL (-) 9 LEELGALWG G L L E|E\A\W\G\L 55.6 5 5 1 1 0 b-ion bias 100.798599 1.510875 987.5128 987.515 -0.0016 -1.6 0.7999999999999998 3.8 1527.7 nuORFdb_human ENST00000209728.8_1_17:38444255-38444294:+ ENST00000209728.8_1_17:38444255-38444294:+ CDC6 5' uORF 5' uORF GN=CDC6 5' uORF E_20221201_NO30_400nL_HLAc1_4e7_PDAC_Slot2-6_1_3524 40mio-rep1 E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep1_Slot1-04_1_3496.28569.347.1 1 1 1 8.68 7.14 1.78 1.54 63.4 5 0 0 34400.0 Hydrogen 5 (R) 9 GLLEEAWGL (-) 9 LEELGALWG G L L E\E|A|W\G\L 55.6 5 5 2 2 0 b-ion bias 98.697586 1.516912 987.5114 987.515 -0.0036 -3.6 -0.3999999999999999 3.8 1527.7 nuORFdb_human ENST00000209728.8_1_17:38444255-38444294:+ ENST00000209728.8_1_17:38444255-38444294:+ CDC6 5' uORF 5' uORF GN=CDC6 5' uORF E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep1_Slot1-04_1_3496 40mio-rep2 E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep2_Slot2-5_1_3520.28485.364.1 1 1 1 9.2 6.1 2.2 3.11 66.4 5 0 0 3260.0 Hydrogen 5 (R) 9 GLLEEAWGL (-) 9 LELAGEWAV G L L E|E\A|W\G\L 55.6 5 5 2 2 0 b-ion bias 98.402908 1.506544 987.508 987.515 -0.0066 -6.7 -1.7999999999999998 3.8 1527.7 nuORFdb_human ENST00000209728.8_1_17:38444255-38444294:+ ENST00000209728.8_1_17:38444255-38444294:+ CDC6 5' uORF 5' uORF GN=CDC6 5' uORF E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep2_Slot2-5_1_3520 40mio-rep3 E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep3_Slot2-5_1_3521.28492.361.1 1 1 1 8.77 6.9 2.67 1.87 65.0 5 0 0 26100.0 Hydrogen 5 (R) 9 GLLEEAWGL (-) 9 LEELGALWG G L L E\E|A|W\G\L 55.6 5 5 2 2 0 b-ion bias 98.419846 1.508355 987.5122 987.515 -0.0026 -2.6 1.9999999999999996 3.8 1527.7 nuORFdb_human ENST00000209728.8_1_17:38444255-38444294:+ ENST00000209728.8_1_17:38444255-38444294:+ CDC6 5' uORF 5' uORF GN=CDC6 5' uORF E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep3_Slot2-5_1_3521 40mio-rep4 E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep4_Slot2-5_1_3522.28493.360.1 1 1 1 9.01 6.21 1.94 2.8 62.3 5 0 0 8680.0 Hydrogen 5 (R) 9 GLLEEAWGL (-) 9 LEELGALWG G L L E|E|A|W\G\L 55.6 5 5 3 3 0 dual b/y bias 98.413536 1.508721 987.5108 987.515 -0.0036 -3.6 1.1 3.8 1527.7 nuORFdb_human ENST00000209728.8_1_17:38444255-38444294:+ ENST00000209728.8_1_17:38444255-38444294:+ CDC6 5' uORF 5' uORF GN=CDC6 5' uORF E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep4_Slot2-5_1_3522 40mio-rep1 E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep1_Slot1-04_1_3496.21681.1485.2 2 1 1 10.44 7.75 2.14 2.69 73.0 6 1 1 4270.0 Hydrogen 16 (P) 9 GLSDKTFLL (Q) 9 GLTFTATGIL G L/S/D/K T\F\L\L 55.6 5 3 3 0 0 unbiased 74.897186 0.823187 497.2822 993.562 -0.0048 -4.8 -1.5999999999999996 5.84 5213.2 nuORFdb_human ENST00000371602.8_1_20:49510960-49518599:- ENST00000371602.8_1_20:49510960-49518599:- ADNP Out-of-Frame Out-of-Frame GN=ADNP Out-of-Frame E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep1_Slot1-04_1_3496 40mio-rep3 E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep3_Slot2-5_1_3521.24587.501.1 1 1 1 9.63 7.17 3.4 2.46 67.7 7 0 0 21500.0 Hydrogen 41 (L) 9 GLVEGITAL (A) 9 LVGELGTAI G L/V/E\G|I\T\A\L 77.8 7 5 3 1 0 unbiased 84.933121 1.425257 872.5056 872.509 -0.0028 -3.2 1.3999999999999995 4.0 6269.3 nuORFdb_human ENST00000502904.5_1_5:179045169-179045340:- ENST00000502904.5_1_5:179045169-179045340:- HNRNPH1 3' Overlap dORF 3' Overlap dORF GN=HNRNPH1 3' Overlap dORF E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep3_Slot2-5_1_3521 40mio-rep1 E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep1_Slot1-04_1_3496.28439.176.1 1 1 1 13.07 1.21 7.99 11.87 87.7 6 0 0 7170.0 Hydrogen 82 (H) 10 GLYEGLNWLA (N) 10 LGGREAGYELA G L Y/E\G L|N\W\L\A 50.0 5 5 2 1 0 b-ion bias 98.248886 1.617255 1135.5738 1135.578 -0.0082 -7.3 -4.1 4.0 11276.4 nuORFdb_human ENST00000455565.5_2_17:43707389-43713807:+ ENST00000455565.5_2_17:43707389-43713807:+|ENST00000444561.2_1_17:43710417-43713807:+|ENST00000585122.5_2_17:43712752-43713807:+ LINC02210 Pseudogene Pseudogene GN=LINC02210 Pseudogene E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep1_Slot1-04_1_3496 40mio-rep2 E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep2_Slot2-5_1_3520.28364.190.1 1 2 1 13.12 2.35 7.76 10.77 85.7 7 0 0 12500.0 Hydrogen 82 (H) 10 GLYEGLNWLA (N) 10 FIAGADINMLA G L Y/E/G\L|N\W\L\A 70.0 7 5 3 1 0 unbiased 97.985222 1.608595 1135.5733 1135.578 -0.0082 -7.3 -2.3999999999999995 4.0 11276.4 nuORFdb_human ENST00000455565.5_2_17:43707389-43713807:+ ENST00000455565.5_2_17:43707389-43713807:+|ENST00000444561.2_1_17:43710417-43713807:+|ENST00000585122.5_2_17:43712752-43713807:+ LINC02210 Pseudogene Pseudogene GN=LINC02210 Pseudogene E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep2_Slot2-5_1_3520 40mio-rep3 E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep3_Slot2-5_1_3521.28363.1383.2 2 3 1 13.54 5.04 8.34 8.5 77.8 7 0 0 20900.0 Hydrogen 82 (H) 10 GLYEGLNWLA (N) 10 QFLMENAVLA G L Y|E\G/L|N|W\L\A 70.0 7 6 4 3 0 unbiased 97.974556 0.887143 568.2897 1135.578 -0.0055 -4.9 -0.3000000000000007 4.0 11276.4 nuORFdb_human ENST00000455565.5_2_17:43707389-43713807:+ ENST00000455565.5_2_17:43707389-43713807:+|ENST00000444561.2_1_17:43710417-43713807:+|ENST00000585122.5_2_17:43712752-43713807:+ LINC02210 Pseudogene Pseudogene GN=LINC02210 Pseudogene E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep3_Slot2-5_1_3521 40mio-rep4 E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep4_Slot2-5_1_3522.28369.1383.2 2 2 1 14.67 4.09 9.42 10.58 82.9 7 0 0 9540.0 Hydrogen 82 (H) 10 GLYEGLNWLA (N) 10 LGGREAGYELA G L Y\E\G\L|N|W\L\A 70.0 7 7 2 2 0 b-ion bias 97.985474 0.886942 568.2915 1135.578 -0.0035 -3.1 1.6 4.0 11276.4 nuORFdb_human ENST00000455565.5_2_17:43707389-43713807:+ ENST00000455565.5_2_17:43707389-43713807:+|ENST00000444561.2_1_17:43710417-43713807:+|ENST00000585122.5_2_17:43712752-43713807:+ LINC02210 Pseudogene Pseudogene GN=LINC02210 Pseudogene E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep4_Slot2-5_1_3522 40mio-rep1 E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep1_Slot1-04_1_3496.26368.430.1 1 1 1 9.64 8.1 0.89 1.53 79.3 5 0 0 3590.0 Hydrogen 12 (L) 9 GLYELPPEV (Q) 9 LIFSnPPDL G L Y E\L|P|P\E\V 55.6 5 5 2 2 0 b-ion bias 91.092079 1.467538 1016.53 1016.53 0.0001 0.1 3.3000000000000003 3.8 5180.0 nuORFdb_human ENST00000553027.1_1_12:49318129-49318267:- ENST00000553027.1_1_12:49318129-49318267:- FKBP11 ncRNA Retained Intron lncRNA GN=FKBP11 ncRNA Retained Intron E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep1_Slot1-04_1_3496 Eva-50mio-3fr AL20210827_EV_HLA_PDAC_PANFRO413_2D_50e6_W632_classI_fxn3.31459.31459.2 2 1 1 15.17 8.9 5.16 6.27 84.1 8 1 1 23500000.0 Hydrogen 4 (A) 10 GPAALRLSAL (R) 10 PGALLSARAL G/P|A|A|L/R\L S|A|L 80.0 8 6 7 5 0 dual b/y bias 0.999 85.6 57.1 100.0 0.0 484.8002 968.589 0.0044 4.5 4.0 9.75 2974.4 nuORFdb_human ENST00000353332.9_1_3:9691400-9695362:+ ENST00000353332.9_1_3:9691400-9695362:+ MTMR14 Out-of-Frame Out-of-Frame GN=MTMR14 Out-of-Frame AL20210827_EV_HLA_PDAC_PANFRO413_2D_50e6_W632_classI_fxn3 SCP E_20221201_NO30_400nL_HLAc1_4e7_PDAC_Slot2-6_1_3524.18393.1487.2 2 2 1 12.91 6.02 8.24 6.89 83.0 9 1 1 14600.0 Hydrogen 4 (A) 10 GPAALRLSAL (R) 10 PAGALRLTLG G/P/A/A|L|R|L\S\A\L 100.0 10 6 6 3 0 unbiased 63.541588 0.821713 484.7971 968.589 -0.002 -2.1 0.2999999999999998 9.75 2974.4 nuORFdb_human ENST00000353332.9_1_3:9691400-9695362:+ ENST00000353332.9_1_3:9691400-9695362:+ MTMR14 Out-of-Frame Out-of-Frame GN=MTMR14 Out-of-Frame E_20221201_NO30_400nL_HLAc1_4e7_PDAC_Slot2-6_1_3524 Exploris_FAIMS VH20221207_MKP_FAIMS2022E_HLAc1_4e7_PDAC.46586.46586.2 2 1 1 10.1 7.3 2.23 2.8 68.5 6 1 1 6810000.0 Hydrogen 4 (A) 10 GPAALRLSAL (R) 10 PGALLSARAL G P|A|A/L/R L S|A|L 50.0 5 4 6 4 0 dual b/y bias 0.999 85.6 83.6 101.3 -50.0 484.7945 968.589 -0.0069 -7.2 -4.6 9.75 2974.4 nuORFdb_human ENST00000353332.9_1_3:9691400-9695362:+ ENST00000353332.9_1_3:9691400-9695362:+ MTMR14 Out-of-Frame Out-of-Frame GN=MTMR14 Out-of-Frame VH20221207_MKP_FAIMS2022E_HLAc1_4e7_PDAC Exploris_FAIMS VH20221207_MKP_FAIMS2022E_HLAc1_4e7_PDAC.13192.13192.2 2 1 1 9.33 7.7 2.11 1.63 93.3 6 1 1 1060000.0 Hydrogen 29 (C) 10 GPSGGRGAAL (R) 10 GPSAVMPAL G/P|S/G/G R G A\A|L 60.0 6 3 5 2 0 unbiased 0.968 78.6 28.62 120.0 -50.0 421.7265 842.448 -0.0022 -2.6 0.0 9.75 4262.7 smORF_v4|nuORFdb_human MAF1_8:145159528-145159650:+ MAF1_8:145159528-145159650:+|ENST00000322428.9_1_8:145159470-145159650:+ MAF1 uORF 8:145159528-145159650:+ 8 MAF1 5' uORF 5' uORF GN=MAF1 VH20221207_MKP_FAIMS2022E_HLAc1_4e7_PDAC SCP E_20221201_NO30_400nL_HLAc1_4e7_PDAC_Slot2-6_1_3524.7946.1555.2 2 1 1 10.1 8.71 2.21 1.39 78.2 7 1 1 12100.0 Hydrogen 72 (C) 9 GPSGRLSSL (L) 9 GGPSRLSSL G/P/S G/R\L|S\S\L 77.8 7 4 4 1 0 unbiased 27.452761 0.77908 437.2408 873.479 -0.0041 -4.7 -2.3000000000000003 9.75 44167.8 nuORFdb_human ENST00000526097.1_1_8:11418120-11421614:+ ENST00000526097.1_1_8:11418120-11421614:+ BLK ncRNA Retained Intron lncRNA GN=BLK ncRNA Retained Intron E_20221201_NO30_400nL_HLAc1_4e7_PDAC_Slot2-6_1_3524 40mio-rep4 E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep4_Slot2-5_1_3522.24460.1412.2 2 1 1 7.78 6.63 0.96 1.15 69.4 5 1 1 6370.0 Hydrogen 10 (T) 9 GREEFFSLI (P) 9 SPASEPHFLL G R E/E F-F\S\L\I 44.4 4 3 1 0 1 b-ion bias 84.482895 0.869116 549.2804 1097.563 -0.0099 -9.0 -4.3 4.53 3462.0 nuORFdb_human ENST00000311403.4_1_17:7387449-7387542:- ENST00000311403.4_1_17:7387449-7387542:- ZBTB4 5' uORF 5' uORF GN=ZBTB4 5' uORF E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep4_Slot2-5_1_3522 40mio-rep1 E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep1_Slot1-04_1_3496.4772.1557.2 2 1 1 8.79 5.47 4.98 3.32 66.6 6 2 2 5390.0 Hydrogen 34 (N) 8 GSGKSLKW (R) 8 SGKKEKW G/S/G K/S\L|K\W 75.0 6 3 4 1 0 unbiased 16.493704 0.777594 431.7398 862.478 -0.0054 -6.3 -3.0999999999999996 10.0 5943.6 nuORFdb_human ENST00000356056.9_1_15:72338569-72338728:- ENST00000356056.9_1_15:72338569-72338728:- MYO9A Out-of-Frame Out-of-Frame GN=MYO9A Out-of-Frame E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep1_Slot1-04_1_3496 10mio-rep1 E_20221201_NO30_400nL_HLAc1_1e7_directIP_titration_rep1_Slot2-3_1_3512.20009.559.1 1 1 1 8.5 3.76 4.98 4.74 69.1 7 0 0 3630.0 Hydrogen 70 (A) 9 GSLGVDEAW (F) 9 AESSPSAVGE G S/L/G\V\D\E\A\W 77.8 7 5 2 0 0 b-ion bias 69.118927 1.390785 933.4249 933.431 -0.0062 -6.7 1.0 3.66 12057.8 nuORFdb_human ENST00000302036.11_2_3:9791692-9792019:+ ENST00000302036.11_2_3:9791692-9792019:+ OGG1 5' Overlap uORF 5' Overlap uORF GN=OGG1 5' Overlap uORF E_20221201_NO30_400nL_HLAc1_1e7_directIP_titration_rep1_Slot2-3_1_3512 20mio-rep1 E_20221201_NO30_400nL_HLAc1_2e7_directIP_titration_rep1_Slot2-4_1_3515.19866.558.1 1 1 1 11.05 6.0 5.9 5.05 76.8 7 0 0 4070.0 Hydrogen 70 (A) 9 GSLGVDEAW (F) 9 EVKGDEAW G S/L/G\V|D\E\A\W 77.8 7 5 3 1 0 unbiased 68.60598 1.391354 933.4237 933.431 -0.0072 -7.8 -1.5999999999999996 3.66 12057.8 nuORFdb_human ENST00000302036.11_2_3:9791692-9792019:+ ENST00000302036.11_2_3:9791692-9792019:+ OGG1 5' Overlap uORF 5' Overlap uORF GN=OGG1 5' Overlap uORF E_20221201_NO30_400nL_HLAc1_2e7_directIP_titration_rep1_Slot2-4_1_3515 20mio-rep2 E_20221201_NO30_400nL_HLAc1_2e7_directIP_titration_rep3_Slot2-4_1_3518.19931.552.1 1 1 1 10.7 3.83 6.78 6.87 71.1 7 0 0 3910.0 Hydrogen 70 (A) 9 GSLGVDEAW (F) 9 KDGLnEAW G S/L/G\V|D\E\A\W 77.8 7 5 3 1 0 unbiased 68.846458 1.395014 933.4278 933.431 -0.0032 -3.5 2.8 3.66 12057.8 nuORFdb_human ENST00000302036.11_2_3:9791692-9792019:+ ENST00000302036.11_2_3:9791692-9792019:+ OGG1 5' Overlap uORF 5' Overlap uORF GN=OGG1 5' Overlap uORF E_20221201_NO30_400nL_HLAc1_2e7_directIP_titration_rep3_Slot2-4_1_3518 20mio-rep3 E_20221201_NO30_400nL_HLAc1_2e7_directIP_titration_rep4_Slot2-4_1_3519.19908.547.1 1 1 1 10.61 6.23 5.16 4.38 70.1 7 0 0 5360.0 Hydrogen 70 (A) 9 GSLGVDEAW (F) 9 EVKGDEAW G S/L/G\V|D\E\A\W 77.8 7 5 3 1 0 unbiased 68.757935 1.397824 933.4265 933.431 -0.0052 -5.6 0.20000000000000018 3.66 12057.8 nuORFdb_human ENST00000302036.11_2_3:9791692-9792019:+ ENST00000302036.11_2_3:9791692-9792019:+ OGG1 5' Overlap uORF 5' Overlap uORF GN=OGG1 5' Overlap uORF E_20221201_NO30_400nL_HLAc1_2e7_directIP_titration_rep4_Slot2-4_1_3519 40mio-rep1 E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep1_Slot1-04_1_3496.20133.526.1 1 2 1 12.16 5.68 6.18 6.48 80.4 7 0 0 33900.0 Hydrogen 70 (A) 9 GSLGVDEAW (F) 9 KDGLnEAW G S/L/G|V|D\E\A\W 77.8 7 5 4 2 0 unbiased 69.549843 1.410614 933.4283 933.431 -0.0032 -3.5 -0.2999999999999998 3.66 12057.8 nuORFdb_human ENST00000302036.11_2_3:9791692-9792019:+ ENST00000302036.11_2_3:9791692-9792019:+ OGG1 5' Overlap uORF 5' Overlap uORF GN=OGG1 5' Overlap uORF E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep1_Slot1-04_1_3496 40mio-rep2 E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep2_Slot2-5_1_3520.19790.546.1 1 3 1 9.38 4.52 5.23 4.86 67.8 7 0 0 16000.0 Hydrogen 70 (A) 9 GSLGVDEAW (F) 9 NLSVEnAW G S/L/G\V|D\E\A\W 77.8 7 5 3 1 0 unbiased 68.365112 1.398733 933.4311 933.431 -0.0002 -0.3 4.6000000000000005 3.66 12057.8 nuORFdb_human ENST00000302036.11_2_3:9791692-9792019:+ ENST00000302036.11_2_3:9791692-9792019:+ OGG1 5' Overlap uORF 5' Overlap uORF GN=OGG1 5' Overlap uORF E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep2_Slot2-5_1_3520 40mio-rep3 E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep3_Slot2-5_1_3521.19814.540.1 1 2 1 10.59 6.45 6.29 4.13 71.7 7 0 0 13000.0 Hydrogen 70 (A) 9 GSLGVDEAW (F) 9 EVKGDEAW G S/L/G\V|D\E\A\W 77.8 7 5 3 1 0 unbiased 68.447136 1.402065 933.4301 933.431 -0.0012 -1.3 3.3 3.66 12057.8 nuORFdb_human ENST00000302036.11_2_3:9791692-9792019:+ ENST00000302036.11_2_3:9791692-9792019:+ OGG1 5' Overlap uORF 5' Overlap uORF GN=OGG1 5' Overlap uORF E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep3_Slot2-5_1_3521 40mio-rep4 E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep4_Slot2-5_1_3522.19801.543.1 1 3 1 9.33 5.73 5.83 3.6 73.9 7 0 0 15000.0 Hydrogen 70 (A) 9 GSLGVDEAW (F) 9 EVKGDEAW G S/L/G\V|D\E\A\W 77.8 7 5 3 1 0 unbiased 68.386009 1.400327 933.4251 933.431 -0.0062 -6.7 -2.0 3.66 12057.8 nuORFdb_human ENST00000302036.11_2_3:9791692-9792019:+ ENST00000302036.11_2_3:9791692-9792019:+ OGG1 5' Overlap uORF 5' Overlap uORF GN=OGG1 5' Overlap uORF E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep4_Slot2-5_1_3522 40mio-rep2 E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep2_Slot2-5_1_3520.19942.1454.2 2 1 1 8.56 8.56 8.56 54.3 8 0 0 2810.0 Hydrogen 10 (M) 9 GSLSLHTPW (V) 9 G/S/L|S|L/H-T|P\W 100.0 9 4 6 3 1 dual b/y bias 68.889923 0.842672 499.2582 997.51 -0.0014 -1.4 3.5000000000000004 6.74 5051.7 nuORFdb_human ENST00000520337.1_1_8:104384717-104384846:+ ENST00000520337.1_1_8:104384717-104384846:+ CTHRC1 5' Overlap uORF 5' Overlap uORF GN=CTHRC1 5' Overlap uORF E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep2_Slot2-5_1_3520 40mio-rep1 E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep1_Slot1-04_1_3496.9549.1541.2 2 1 1 8.11 5.26 2.71 2.85 70.2 6 0 0 5590.0 Hydrogen 28 (C) 8 GSSESHLW (F) 8 HGSVSGQSSG G/S/S/E/S H/L\W 75.0 6 1 5 0 0 y-ion bias 32.988918 0.787471 451.7012 902.4 -0.0055 -6.1 -2.8999999999999995 5.24 4679.3 nuORFdb_human ENST00000494222.5_1_10:124134272-124152763:+ ENST00000494222.5_1_10:124134272-124152763:+|ENST00000368989.6_2_10:124134301-124152763:+|ENST00000463663.6_1_10:124134413-124152763:+|ENST00000392799.7_2_10:124147492-124152763:+|ENST00000368988.5_2_10:124152673-124152763:+ PLEKHA1 5' Overlap uORF 5' Overlap uORF GN=PLEKHA1 5' Overlap uORF E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep1_Slot1-04_1_3496 40mio-rep1 E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep1_Slot1-04_1_3496.8789.1657.3 3 1 1 8.46 6.33 3.24 2.13 67.8 6 1 2 4220.0 C14c Hydrogen 7 (S) 10 GSVLERRcTW (R) 10 GSVLLTcDAEAK G/S/V/L|E/R R c T\W 60.0 6 2 5 1 0 y-ion bias c:Cysteinylation 30.36504 0.714321 442.5405 1263.626 61.9792 -2.6 0.6000000000000001 9.6 2785.2 nuORFdb_human ENST00000358739.4_1_6:27776096-27776165:+ ENST00000358739.4_1_6:27776096-27776165:+ HIST1H2AI Out-of-Frame Out-of-Frame GN=HIST1H2AI Out-of-Frame E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep1_Slot1-04_1_3496 10mio-rep1 E_20221201_NO30_400nL_HLAc1_1e7_directIP_titration_rep1_Slot2-3_1_3512.20229.594.1 1 1 1 11.18 5.52 5.29 5.66 87.5 6 0 0 4260.0 Hydrogen 80 (A) 9 GSVPTVGEW (R) 9 DKKPEEW G S/V/P T/V\G\E\W 55.6 5 3 3 0 0 unbiased 69.878265 1.370146 931.4447 931.452 -0.007 -7.5 0.20000000000000018 4.0 10367.6 nuORFdb_human ENST00000300249.9_1_18:32621372-32650196:+ ENST00000300249.9_1_18:32621372-32650196:+ MAPRE2 5' Overlap uORF 5' Overlap uORF GN=MAPRE2 5' Overlap uORF E_20221201_NO30_400nL_HLAc1_1e7_directIP_titration_rep1_Slot2-3_1_3512 10mio-rep2 E_20221201_NO30_400nL_HLAc1_1e7_directIP_titration_rep2_Slot2-3_1_3513.20151.594.1 1 1 1 12.45 6.21 5.98 6.24 89.6 7 0 0 2380.0 Hydrogen 80 (A) 9 GSVPTVGEW (R) 9 KnPEKEW G S/V/P\T|V\G\E\W 77.8 7 5 3 1 0 unbiased 69.6035 1.370101 931.4488 931.452 -0.003 -3.2 3.5 4.0 10367.6 nuORFdb_human ENST00000300249.9_1_18:32621372-32650196:+ ENST00000300249.9_1_18:32621372-32650196:+ MAPRE2 5' Overlap uORF 5' Overlap uORF GN=MAPRE2 5' Overlap uORF E_20221201_NO30_400nL_HLAc1_1e7_directIP_titration_rep2_Slot2-3_1_3513 10mio-rep3 E_20221201_NO30_400nL_HLAc1_1e7_directIP_titration_rep3_Slot2-3_1_3514.20125.590.1 1 1 1 11.13 4.89 6.22 6.24 86.4 6 0 0 3850.0 Hydrogen 80 (A) 9 GSVPTVGEW (R) 9 AGLAEVGEnA G S/V/P T|V\G\E\W 55.6 5 4 3 1 0 unbiased 69.517853 1.372329 931.4478 931.452 -0.004 -4.3 2.0 4.0 10367.6 nuORFdb_human ENST00000300249.9_1_18:32621372-32650196:+ ENST00000300249.9_1_18:32621372-32650196:+ MAPRE2 5' Overlap uORF 5' Overlap uORF GN=MAPRE2 5' Overlap uORF E_20221201_NO30_400nL_HLAc1_1e7_directIP_titration_rep3_Slot2-3_1_3514 20mio-rep1 E_20221201_NO30_400nL_HLAc1_2e7_directIP_titration_rep1_Slot2-4_1_3515.20112.591.1 1 1 1 11.04 4.19 5.98 6.85 81.5 6 0 0 6780.0 Hydrogen 80 (A) 9 GSVPTVGEW (R) 9 KnPEKEW G S/V/P T|V|G\E\W 55.6 5 4 4 2 0 unbiased 69.455498 1.371613 931.4455 931.452 -0.007 -7.5 -1.2999999999999998 4.0 10367.6 nuORFdb_human ENST00000300249.9_1_18:32621372-32650196:+ ENST00000300249.9_1_18:32621372-32650196:+ MAPRE2 5' Overlap uORF 5' Overlap uORF GN=MAPRE2 5' Overlap uORF E_20221201_NO30_400nL_HLAc1_2e7_directIP_titration_rep1_Slot2-4_1_3515 20mio-rep2 E_20221201_NO30_400nL_HLAc1_2e7_directIP_titration_rep3_Slot2-4_1_3518.20156.587.1 1 1 1 12.12 5.72 6.37 6.4 85.2 7 0 0 6540.0 Hydrogen 80 (A) 9 GSVPTVGEW (R) 9 KnPEKEW G S/V/P\T|V\G\E\W 77.8 7 5 3 1 0 unbiased 69.623932 1.374212 931.4455 931.452 -0.007 -7.5 -1.2000000000000002 4.0 10367.6 nuORFdb_human ENST00000300249.9_1_18:32621372-32650196:+ ENST00000300249.9_1_18:32621372-32650196:+ MAPRE2 5' Overlap uORF 5' Overlap uORF GN=MAPRE2 5' Overlap uORF E_20221201_NO30_400nL_HLAc1_2e7_directIP_titration_rep3_Slot2-4_1_3518 20mio-rep3 E_20221201_NO30_400nL_HLAc1_2e7_directIP_titration_rep4_Slot2-4_1_3519.20151.584.1 1 2 1 11.94 5.1 6.85 6.84 90.2 6 0 0 6040.0 Hydrogen 80 (A) 9 GSVPTVGEW (R) 9 KnPEKEW G S/V/P T|V\G\E\W 55.6 5 4 3 1 0 unbiased 69.596672 1.376018 931.4464 931.452 -0.006 -6.4 -0.6000000000000005 4.0 10367.6 nuORFdb_human ENST00000300249.9_1_18:32621372-32650196:+ ENST00000300249.9_1_18:32621372-32650196:+ MAPRE2 5' Overlap uORF 5' Overlap uORF GN=MAPRE2 5' Overlap uORF E_20221201_NO30_400nL_HLAc1_2e7_directIP_titration_rep4_Slot2-4_1_3519 40mio-rep1 E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep1_Slot1-04_1_3496.20306.565.1 1 3 1 12.39 6.2 6.72 6.19 88.7 6 0 0 28400.0 Hydrogen 80 (A) 9 GSVPTVGEW (R) 9 KnPEKEW G S/V/P T|V\G\E\W 55.6 5 4 3 1 0 unbiased 70.147163 1.387163 931.447 931.452 -0.005 -5.3 -2.0999999999999996 4.0 10367.6 nuORFdb_human ENST00000300249.9_1_18:32621372-32650196:+ ENST00000300249.9_1_18:32621372-32650196:+ MAPRE2 5' Overlap uORF 5' Overlap uORF GN=MAPRE2 5' Overlap uORF E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep1_Slot1-04_1_3496 40mio-rep2 E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep2_Slot2-5_1_3520.20032.582.1 1 3 1 12.73 7.06 4.64 5.67 86.6 7 0 0 21300.0 Hydrogen 80 (A) 9 GSVPTVGEW (R) 9 KnPEKEW G S/V/P/T/V\G|E\W 77.8 7 3 5 1 0 unbiased 69.200645 1.376946 931.4471 931.452 -0.005 -5.3 -0.39999999999999947 4.0 10367.6 nuORFdb_human ENST00000300249.9_1_18:32621372-32650196:+ ENST00000300249.9_1_18:32621372-32650196:+ MAPRE2 5' Overlap uORF 5' Overlap uORF GN=MAPRE2 5' Overlap uORF E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep2_Slot2-5_1_3520 40mio-rep3 E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep3_Slot2-5_1_3521.20040.578.1 1 2 1 12.27 6.52 5.74 5.75 88.1 7 0 0 11900.0 Hydrogen 80 (A) 9 GSVPTVGEW (R) 9 KnPEKEW G S/V/P/T|V\G\E\W 77.8 7 4 4 1 0 unbiased 69.228188 1.379519 931.4465 931.452 -0.006 -6.4 -1.8000000000000007 4.0 10367.6 nuORFdb_human ENST00000300249.9_1_18:32621372-32650196:+ ENST00000300249.9_1_18:32621372-32650196:+ MAPRE2 5' Overlap uORF 5' Overlap uORF GN=MAPRE2 5' Overlap uORF E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep3_Slot2-5_1_3521 40mio-rep4 E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep4_Slot2-5_1_3522.20042.579.1 1 4 1 13.3 6.05 7.65 7.26 88.3 7 0 0 14900.0 Hydrogen 80 (A) 9 GSVPTVGEW (R) 9 TDKPVmAPG G S/V/P\T|V\G\E\W 77.8 7 5 3 1 0 unbiased 69.218002 1.378984 931.4486 931.452 -0.003 -3.2 1.5 4.0 10367.6 nuORFdb_human ENST00000300249.9_1_18:32621372-32650196:+ ENST00000300249.9_1_18:32621372-32650196:+ MAPRE2 5' Overlap uORF 5' Overlap uORF GN=MAPRE2 5' Overlap uORF E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep4_Slot2-5_1_3522 5mio-rep1 E_20221201_NO30_400nL_HLAc1_5e6_directIP_titration_rep1_Slot1-37_1_3502.20450.579.1 1 1 1 9.29 3.73 5.19 5.56 76.8 6 0 0 2180.0 Hydrogen 80 (A) 9 GSVPTVGEW (R) 9 QPPLSADFG G S/V/P T|V\G-E\W 55.6 5 3 3 1 1 unbiased 70.632484 1.378772 931.4408 931.452 -0.011 -11.8 -4.800000000000001 4.0 10367.6 nuORFdb_human ENST00000300249.9_1_18:32621372-32650196:+ ENST00000300249.9_1_18:32621372-32650196:+ MAPRE2 5' Overlap uORF 5' Overlap uORF GN=MAPRE2 5' Overlap uORF E_20221201_NO30_400nL_HLAc1_5e6_directIP_titration_rep1_Slot1-37_1_3502 20mio-rep2 E_20221201_NO30_400nL_HLAc1_2e7_directIP_titration_rep3_Slot2-4_1_3518.19327.601.1 1 1 1 9.77 5.37 5.33 4.41 80.1 7 0 0 2180.0 Hydrogen 54 (Q) 9 GSVPVSPAW (A) 9 VSWSPVPGA G S/V/P\V|S|P-A\W 77.8 7 4 4 2 1 unbiased 66.7612 1.365885 899.4585 899.462 -0.0031 -3.5 2.8 5.52 12248.8 nuORFdb_human TCONS_00025884_17:27894303-27895505:+ TCONS_00025884_17:27894303-27895505:+|TCONS_00025885_17:27894992-27895505:+|ENST00000578749.5_2_17:27895268-27895505:+|TCONS_00025886_17:27895125-27895505:+ linc-TP53I13 5' uORF 5' uORF GN=linc-TP53I13 5' uORF E_20221201_NO30_400nL_HLAc1_2e7_directIP_titration_rep3_Slot2-4_1_3518 20mio-rep3 E_20221201_NO30_400nL_HLAc1_2e7_directIP_titration_rep4_Slot2-4_1_3519.19315.604.1 1 1 1 7.67 3.77 3.32 3.9 77.5 5 0 0 2480.0 Hydrogen 54 (Q) 9 GSVPVSPAW (A) 9 GNLLEEQP G S V/P V/S|P\A\W 44.4 4 3 3 1 0 unbiased 66.709938 1.363842 899.4592 899.462 -0.0031 -3.5 2.3 5.52 12248.8 nuORFdb_human TCONS_00025884_17:27894303-27895505:+ TCONS_00025884_17:27894303-27895505:+|TCONS_00025885_17:27894992-27895505:+|ENST00000578749.5_2_17:27895268-27895505:+|TCONS_00025886_17:27895125-27895505:+ linc-TP53I13 5' uORF 5' uORF GN=linc-TP53I13 5' uORF E_20221201_NO30_400nL_HLAc1_2e7_directIP_titration_rep4_Slot2-4_1_3519 40mio-rep1 E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep1_Slot1-04_1_3496.19487.582.1 1 1 1 9.89 6.78 1.39 3.11 83.5 6 0 0 5010.0 Hydrogen 54 (Q) 9 GSVPVSPAW (A) 9 PFQVSPQP G S V/P\V|S|P\A-W 66.7 6 4 3 2 1 unbiased 67.317833 1.376869 899.4585 899.462 -0.0041 -4.6 -1.3999999999999995 5.52 12248.8 nuORFdb_human TCONS_00025884_17:27894303-27895505:+ TCONS_00025884_17:27894303-27895505:+|TCONS_00025885_17:27894992-27895505:+|ENST00000578749.5_2_17:27895268-27895505:+|TCONS_00025886_17:27895125-27895505:+ linc-TP53I13 5' uORF 5' uORF GN=linc-TP53I13 5' uORF E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep1_Slot1-04_1_3496 40mio-rep2 E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep2_Slot2-5_1_3520.19206.599.1 1 2 1 8.56 3.29 5.07 5.27 75.1 7 0 0 7730.0 Hydrogen 54 (Q) 9 GSVPVSPAW (A) 9 PLAASSPAW G S/V/P\V/S|P\A\W 77.8 7 4 4 1 0 unbiased 66.347923 1.367051 899.4584 899.462 -0.0041 -4.6 0.3000000000000007 5.52 12248.8 nuORFdb_human TCONS_00025884_17:27894303-27895505:+ TCONS_00025884_17:27894303-27895505:+|TCONS_00025885_17:27894992-27895505:+|ENST00000578749.5_2_17:27895268-27895505:+|TCONS_00025886_17:27895125-27895505:+ linc-TP53I13 5' uORF 5' uORF GN=linc-TP53I13 5' uORF E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep2_Slot2-5_1_3520 40mio-rep3 E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep3_Slot2-5_1_3521.19226.592.1 1 2 1 8.92 5.7 3.84 3.22 80.6 6 0 0 5150.0 Hydrogen 54 (Q) 9 GSVPVSPAW (A) 9 VSWSPVPGA G S/V/P V|S|P-A\W 55.6 5 3 4 2 1 unbiased 66.414093 1.371175 899.4581 899.462 -0.0041 -4.6 0.0 5.52 12248.8 nuORFdb_human TCONS_00025884_17:27894303-27895505:+ TCONS_00025884_17:27894303-27895505:+|TCONS_00025885_17:27894992-27895505:+|ENST00000578749.5_2_17:27895268-27895505:+|TCONS_00025886_17:27895125-27895505:+ linc-TP53I13 5' uORF 5' uORF GN=linc-TP53I13 5' uORF E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep3_Slot2-5_1_3521 40mio-rep4 E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep4_Slot2-5_1_3522.19202.597.1 1 1 1 7.71 4.39 3.63 3.32 75.9 6 0 0 3530.0 Hydrogen 54 (Q) 9 GSVPVSPAW (A) 9 VSWSPVPGA G S/V/P V/S|P-A\W 55.6 5 2 4 1 1 y-ion bias 66.314018 1.368159 899.4598 899.462 -0.0021 -2.4 2.3000000000000003 5.52 12248.8 nuORFdb_human TCONS_00025884_17:27894303-27895505:+ TCONS_00025884_17:27894303-27895505:+|TCONS_00025885_17:27894992-27895505:+|ENST00000578749.5_2_17:27895268-27895505:+|TCONS_00025886_17:27895125-27895505:+ linc-TP53I13 5' uORF 5' uORF GN=linc-TP53I13 5' uORF E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep4_Slot2-5_1_3522 40mio-rep1 E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep1_Slot1-04_1_3496.22210.1257.2 2 1 1 9.69 -3.07 10.23 12.76 72.1 6 0 0 3440.0 Hydrogen 90 (A) 11 GTEDNYTYWAY (L) 11 YGnDPDIEMAWA G T E D N|Y|T\Y|W\A\Y 54.5 6 6 3 3 0 b-ion bias 76.724037 0.965527 691.7796 1382.553 -0.0008 -0.6 2.6 3.67 12556.4 nuORFdb_human ENST00000605830.5_1_3:129624520-129624853:+ ENST00000605830.5_1_3:129624520-129624853:+ TMCC1-AS1 Antisense lncRNA GN=TMCC1-AS1 Antisense E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep1_Slot1-04_1_3496 40mio-rep3 E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep3_Slot2-5_1_3521.21962.1264.2 2 1 1 8.99 8.99 8.99 69.9 6 0 0 3740.0 Hydrogen 90 (A) 11 GTEDNYTYWAY (L) 11 G T E D N\Y|T\Y|W|A\Y 54.5 6 6 3 3 0 b-ion bias 75.86731 0.961128 691.7788 1382.553 -0.0028 -2.0 2.5999999999999996 3.67 12556.4 nuORFdb_human ENST00000605830.5_1_3:129624520-129624853:+ ENST00000605830.5_1_3:129624520-129624853:+ TMCC1-AS1 Antisense lncRNA GN=TMCC1-AS1 Antisense E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep3_Slot2-5_1_3521 40mio-rep1 E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep1_Slot1-04_1_3496.25320.1317.2 2 2 1 10.43 3.55 5.45 6.88 76.0 5 0 0 8120.0 Hydrogen 111 (G) 10 GTESYPFWNY (I) 10 TTGYDMARnAY G T E S|Y|P F|W-N\Y 40.0 4 4 3 3 1 unbiased 87.47081 0.928021 632.2678 1263.532 -0.003 -2.3 0.9000000000000004 4.0 14895.6 nuORFdb_human ENST00000514615.5_3_12:112123944-112140026:+ ENST00000514615.5_3_12:112123944-112140026:+|ENST00000509936.5_1_12:112123944-112140026:+ ACAD10 5' Overlap uORF 5' Overlap uORF GN=ACAD10 5' Overlap uORF E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep1_Slot1-04_1_3496 40mio-rep2 E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep2_Slot2-5_1_3520.25075.1341.2 2 1 1 9.52 2.41 5.74 7.11 73.2 5 0 0 4230.0 Hydrogen 111 (G) 10 GTESYPFWNY (I) 10 HSAGTNGTGVSmE G T E S/Y|P F|W|N-Y 40.0 4 3 4 3 1 unbiased 86.620651 0.9134 632.2651 1263.532 -0.009 -7.1 -2.1999999999999993 4.0 14895.6 nuORFdb_human ENST00000514615.5_3_12:112123944-112140026:+ ENST00000514615.5_3_12:112123944-112140026:+|ENST00000509936.5_1_12:112123944-112140026:+ ACAD10 5' Overlap uORF 5' Overlap uORF GN=ACAD10 5' Overlap uORF E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep2_Slot2-5_1_3520 40mio-rep3 E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep3_Slot2-5_1_3521.25092.1338.2 2 2 1 14.62 3.49 10.23 11.12 81.6 7 0 0 5160.0 Hydrogen 111 (G) 10 GTESYPFWNY (I) 10 nYTSAPHCLEA G T/E S|Y|P\F|W|N\Y 60.0 6 6 5 4 0 dual b/y bias 86.677498 0.915052 632.2654 1263.532 -0.009 -7.1 -2.5 4.0 14895.6 nuORFdb_human ENST00000514615.5_3_12:112123944-112140026:+ ENST00000514615.5_3_12:112123944-112140026:+|ENST00000509936.5_1_12:112123944-112140026:+ ACAD10 5' Overlap uORF 5' Overlap uORF GN=ACAD10 5' Overlap uORF E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep3_Slot2-5_1_3521 40mio-rep4 E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep4_Slot2-5_1_3522.25092.1336.2 2 2 1 9.6 3.56 6.41 6.04 68.4 7 0 0 7260.0 Hydrogen 111 (G) 10 GTESYPFWNY (I) 10 qDPDVGTnTVSF G T E\S\Y|P\F/W|N-Y 70.0 7 5 3 2 1 unbiased 86.668297 0.916074 632.2671 1263.532 -0.005 -3.9 0.8000000000000003 4.0 14895.6 nuORFdb_human ENST00000514615.5_3_12:112123944-112140026:+ ENST00000514615.5_3_12:112123944-112140026:+|ENST00000509936.5_1_12:112123944-112140026:+ ACAD10 5' Overlap uORF 5' Overlap uORF GN=ACAD10 5' Overlap uORF E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep4_Slot2-5_1_3522 20mio-rep1 E_20221201_NO30_400nL_HLAc1_2e7_directIP_titration_rep1_Slot2-4_1_3515.7044.1502.2 2 1 1 8.15 3.38 5.87 4.78 68.4 6 1 1 6620.0 Hydrogen 21 (I) 9 GTIQKDSNW (M) 9 SALQSInEW G/T/I/Q|K D S|N\W 66.7 6 3 5 2 0 unbiased 24.33654 0.812068 524.7534 1048.506 -0.0071 -6.7 -0.5 5.84 4994.6 nuORFdb_human ENST00000322602.9_1_5:153429475-153432512:+ ENST00000322602.9_1_5:153429475-153432512:+|ENST00000519928.1_1_5:153429472-153431302:+ MFAP3 Out-of-Frame Out-of-Frame GN=MFAP3 Out-of-Frame E_20221201_NO30_400nL_HLAc1_2e7_directIP_titration_rep1_Slot2-4_1_3515 40mio-rep1 E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep1_Slot1-04_1_3496.7331.1485.2 2 1 1 13.23 8.15 7.75 5.07 85.5 7 1 1 10700.0 Hydrogen 21 (I) 9 GTIQKDSNW (M) 9 SALQSInEW G/T/I/Q|K\D S\N\W 77.8 7 4 4 1 0 unbiased 25.331442 0.822719 524.7546 1048.506 -0.0031 -2.9 0.30000000000000027 5.84 4994.6 nuORFdb_human ENST00000322602.9_1_5:153429475-153432512:+ ENST00000322602.9_1_5:153429475-153432512:+|ENST00000519928.1_1_5:153429472-153431302:+ MFAP3 Out-of-Frame Out-of-Frame GN=MFAP3 Out-of-Frame E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep1_Slot1-04_1_3496 40mio-rep3 E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep3_Slot2-5_1_3521.7065.1497.2 2 1 1 14.7 9.25 7.94 5.45 88.9 8 1 1 11900.0 Hydrogen 21 (I) 9 GTIQKDSNW (M) 9 SALQSInEW G/T/I/Q|K\D\S|N\W 100.0 9 5 5 2 0 unbiased 24.408106 0.815305 524.7536 1048.506 -0.0051 -4.8 -0.20000000000000018 5.84 4994.6 nuORFdb_human ENST00000322602.9_1_5:153429475-153432512:+ ENST00000322602.9_1_5:153429475-153432512:+|ENST00000519928.1_1_5:153429472-153431302:+ MFAP3 Out-of-Frame Out-of-Frame GN=MFAP3 Out-of-Frame E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep3_Slot2-5_1_3521 40mio-rep4 E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep4_Slot2-5_1_3522.7055.1496.2 2 1 1 11.33 5.95 4.71 5.38 83.0 7 1 1 11100.0 Hydrogen 21 (I) 9 GTIQKDSNW (M) 9 SALQSInEW G T/I|Q|K\D\S\N\W 77.8 7 6 3 2 0 b-ion bias 24.369329 0.816314 524.754 1048.506 -0.0051 -4.8 -0.09999999999999964 5.84 4994.6 nuORFdb_human ENST00000322602.9_1_5:153429475-153432512:+ ENST00000322602.9_1_5:153429475-153432512:+|ENST00000519928.1_1_5:153429472-153431302:+ MFAP3 Out-of-Frame Out-of-Frame GN=MFAP3 Out-of-Frame E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep4_Slot2-5_1_3522 10mio-rep1 E_20221201_NO30_400nL_HLAc1_1e7_directIP_titration_rep1_Slot2-3_1_3512.14878.1475.2 2 2 1 13.08 9.32 3.72 3.76 86.2 7 1 1 8300.0 Hydrogen 78 (E) 9 GTISSLRSW (L) 9 TGLSRLSSW G T/I/S|S|L/R|S\W 77.8 7 4 6 3 0 dual b/y bias 51.398457 0.828965 503.7651 1006.532 -0.0089 -8.8 -1.1000000000000005 9.75 12856.4 nuORFdb_human ENST00000533310.5_2_11:68031188-68039126:- ENST00000533310.5_2_11:68031188-68039126:-|ENST00000304271.10_2_11:68031188-68039337:- C11orf24 5' Overlap uORF 5' Overlap uORF GN=C11orf24 5' Overlap uORF E_20221201_NO30_400nL_HLAc1_1e7_directIP_titration_rep1_Slot2-3_1_3512 10mio-rep2 E_20221201_NO30_400nL_HLAc1_1e7_directIP_titration_rep2_Slot2-3_1_3513.14838.1476.2 2 1 1 9.9 7.12 3.24 2.77 77.3 7 1 1 4600.0 Hydrogen 78 (E) 9 GTISSLRSW (L) 9 TGLSRLSSW G T/I/S|S/L/R|S\W 77.8 7 3 6 2 0 y-ion bias 51.257797 0.828772 503.7668 1006.532 -0.0049 -4.9 1.7999999999999998 9.75 12856.4 nuORFdb_human ENST00000533310.5_2_11:68031188-68039126:- ENST00000533310.5_2_11:68031188-68039126:-|ENST00000304271.10_2_11:68031188-68039337:- C11orf24 5' Overlap uORF 5' Overlap uORF GN=C11orf24 5' Overlap uORF E_20221201_NO30_400nL_HLAc1_1e7_directIP_titration_rep2_Slot2-3_1_3513 10mio-rep3 E_20221201_NO30_400nL_HLAc1_1e7_directIP_titration_rep3_Slot2-3_1_3514.14785.1473.2 2 2 1 13.05 9.79 3.16 3.27 85.5 7 1 1 10400.0 Hydrogen 78 (E) 9 GTISSLRSW (L) 9 TGLSRLSSW G T/I|S|S/L/R|S\W 77.8 7 4 6 3 0 dual b/y bias 51.077084 0.830288 503.7668 1006.532 -0.0049 -4.9 1.3999999999999995 9.75 12856.4 nuORFdb_human ENST00000533310.5_2_11:68031188-68039126:- ENST00000533310.5_2_11:68031188-68039126:-|ENST00000304271.10_2_11:68031188-68039337:- C11orf24 5' Overlap uORF 5' Overlap uORF GN=C11orf24 5' Overlap uORF E_20221201_NO30_400nL_HLAc1_1e7_directIP_titration_rep3_Slot2-3_1_3514 20mio-rep2 E_20221201_NO30_400nL_HLAc1_2e7_directIP_titration_rep3_Slot2-4_1_3518.14861.1474.2 2 1 1 10.98 7.77 1.63 3.2 82.8 7 1 1 3870.0 Hydrogen 78 (E) 9 GTISSLRSW (L) 9 TGLSRLSSW G T/I|S|S/L|R\S\W 77.8 7 5 5 3 0 dual b/y bias 51.331009 0.829748 503.7654 1006.532 -0.0089 -8.8 -2.500000000000001 9.75 12856.4 nuORFdb_human ENST00000533310.5_2_11:68031188-68039126:- ENST00000533310.5_2_11:68031188-68039126:-|ENST00000304271.10_2_11:68031188-68039337:- C11orf24 5' Overlap uORF 5' Overlap uORF GN=C11orf24 5' Overlap uORF E_20221201_NO30_400nL_HLAc1_2e7_directIP_titration_rep3_Slot2-4_1_3518 20mio-rep3 E_20221201_NO30_400nL_HLAc1_2e7_directIP_titration_rep4_Slot2-4_1_3519.14785.1467.2 2 2 1 12.68 9.46 3.15 3.22 87.4 7 1 1 9450.0 Hydrogen 78 (E) 9 GTISSLRSW (L) 9 TGLSRLSSW G T/I/S|S/L/R|S\W 77.8 7 3 6 2 0 y-ion bias 51.067886 0.834376 503.7657 1006.532 -0.0069 -6.9 -1.1000000000000005 9.75 12856.4 nuORFdb_human ENST00000533310.5_2_11:68031188-68039126:- ENST00000533310.5_2_11:68031188-68039126:-|ENST00000304271.10_2_11:68031188-68039337:- C11orf24 5' Overlap uORF 5' Overlap uORF GN=C11orf24 5' Overlap uORF E_20221201_NO30_400nL_HLAc1_2e7_directIP_titration_rep4_Slot2-4_1_3519 40mio-rep1 E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep1_Slot1-04_1_3496.15017.1441.2 2 1 1 14.59 11.25 3.24 3.33 86.9 7 1 1 39100.0 Hydrogen 78 (E) 9 GTISSLRSW (L) 9 TGLSRLSSW G T/I|S|S/L/R|S\W 77.8 7 4 6 3 0 dual b/y bias 51.873131 0.850873 503.7684 1006.532 -0.0029 -2.9 0.30000000000000027 9.75 12856.4 nuORFdb_human ENST00000533310.5_2_11:68031188-68039126:- ENST00000533310.5_2_11:68031188-68039126:-|ENST00000304271.10_2_11:68031188-68039337:- C11orf24 5' Overlap uORF 5' Overlap uORF GN=C11orf24 5' Overlap uORF E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep1_Slot1-04_1_3496 40mio-rep3 E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep3_Slot2-5_1_3521.14713.1462.2 2 1 1 13.53 8.68 4.28 4.85 86.3 8 1 1 11800.0 Hydrogen 78 (E) 9 GTISSLRSW (L) 9 TGLSRLSSW G/T/I/S|S/L/R|S\W 100.0 9 3 7 2 0 y-ion bias 50.827267 0.837289 503.769 1006.532 -0.0009 -0.9 3.6999999999999997 9.75 12856.4 nuORFdb_human ENST00000533310.5_2_11:68031188-68039126:- ENST00000533310.5_2_11:68031188-68039126:-|ENST00000304271.10_2_11:68031188-68039337:- C11orf24 5' Overlap uORF 5' Overlap uORF GN=C11orf24 5' Overlap uORF E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep3_Slot2-5_1_3521 40mio-rep4 E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep4_Slot2-5_1_3522.14699.1463.2 2 1 1 12.88 9.98 3.31 2.9 82.8 7 1 1 13800.0 Hydrogen 78 (E) 9 GTISSLRSW (L) 9 TGLSRLSSW G T/I|S|S/L/R|S\W 77.8 7 4 6 3 0 dual b/y bias 50.764519 0.836815 503.7669 1006.532 -0.0049 -4.9 -0.20000000000000018 9.75 12856.4 nuORFdb_human ENST00000533310.5_2_11:68031188-68039126:- ENST00000533310.5_2_11:68031188-68039126:-|ENST00000304271.10_2_11:68031188-68039337:- C11orf24 5' Overlap uORF 5' Overlap uORF GN=C11orf24 5' Overlap uORF E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep4_Slot2-5_1_3522 10mio-rep1 E_20221201_NO30_400nL_HLAc1_1e7_directIP_titration_rep1_Slot2-3_1_3512.7254.1411.2 2 1 1 10.92 6.72 6.47 4.2 70.7 7 1 1 4940.0 Hydrogen 25 (L) 9 GTLTTKTHW (-) 9 ASLTTAPPTW G T/L/T/T/K/T|H\W 77.8 7 2 6 1 0 y-ion bias 25.068146 0.869313 522.7733 1044.547 -0.0085 -8.2 -0.4999999999999991 8.76 3748.3 nuORFdb_human T345058_8:65675913-65676012:+ T345058_8:65675913-65676012:+ lincRNA lncRNA GN=nan lincRNA E_20221201_NO30_400nL_HLAc1_1e7_directIP_titration_rep1_Slot2-3_1_3512 10mio-rep3 E_20221201_NO30_400nL_HLAc1_1e7_directIP_titration_rep3_Slot2-3_1_3514.7228.1413.2 2 2 1 9.69 4.43 6.28 5.26 67.9 7 1 1 7040.0 Hydrogen 25 (L) 9 GTLTTKTHW (-) 9 ASLTTAPPTW G T/L/T|T/K/T/H\W 77.8 7 2 6 1 0 y-ion bias 24.976696 0.868026 522.7741 1044.547 -0.0065 -6.3 0.0 8.76 3748.3 nuORFdb_human T345058_8:65675913-65676012:+ T345058_8:65675913-65676012:+ lincRNA lncRNA GN=nan lincRNA E_20221201_NO30_400nL_HLAc1_1e7_directIP_titration_rep3_Slot2-3_1_3514 20mio-rep1 E_20221201_NO30_400nL_HLAc1_2e7_directIP_titration_rep1_Slot2-4_1_3515.7120.1413.2 2 1 1 13.76 8.59 7.87 5.17 76.2 7 1 1 9260.0 Hydrogen 25 (L) 9 GTLTTKTHW (-) 9 ASLTTKYGGF G/T/L|T|T K/T\H\W 77.8 7 4 5 2 0 unbiased 24.598856 0.86821 522.773 1044.547 -0.0085 -8.2 -1.9999999999999991 8.76 3748.3 nuORFdb_human T345058_8:65675913-65676012:+ T345058_8:65675913-65676012:+ lincRNA lncRNA GN=nan lincRNA E_20221201_NO30_400nL_HLAc1_2e7_directIP_titration_rep1_Slot2-4_1_3515 20mio-rep2 E_20221201_NO30_400nL_HLAc1_2e7_directIP_titration_rep3_Slot2-4_1_3518.7286.1409.2 2 1 1 13.98 9.47 5.08 4.51 82.7 7 1 1 6350.0 Hydrogen 25 (L) 9 GTLTTKTHW (-) 9 GTLPGKGAGTW G/T/L/T/T K/T/H\W 77.8 7 1 6 0 0 y-ion bias 25.172853 0.870847 522.7732 1044.547 -0.0085 -8.2 -1.8999999999999995 8.76 3748.3 nuORFdb_human T345058_8:65675913-65676012:+ T345058_8:65675913-65676012:+ lincRNA lncRNA GN=nan lincRNA E_20221201_NO30_400nL_HLAc1_2e7_directIP_titration_rep3_Slot2-4_1_3518 20mio-rep3 E_20221201_NO30_400nL_HLAc1_2e7_directIP_titration_rep4_Slot2-4_1_3519.7170.1405.2 2 3 1 10.86 5.79 6.43 5.07 72.2 7 1 1 18800.0 Hydrogen 25 (L) 9 GTLTTKTHW (-) 9 ASLTTKYGGF G/T/L/T|T/K T|H\W 77.8 7 3 6 2 0 y-ion bias 24.769745 0.872949 522.774 1044.547 -0.0065 -6.3 -0.5 8.76 3748.3 nuORFdb_human T345058_8:65675913-65676012:+ T345058_8:65675913-65676012:+ lincRNA lncRNA GN=nan lincRNA E_20221201_NO30_400nL_HLAc1_2e7_directIP_titration_rep4_Slot2-4_1_3519 40mio-rep1 E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep1_Slot1-04_1_3496.7398.1379.2 2 2 1 18.33 11.59 8.35 6.75 94.8 8 1 1 55600.0 Hydrogen 25 (L) 9 GTLTTKTHW (-) 9 ASLTTAPPTW G/T/L/T|T/K/T|H\W 100.0 9 3 7 2 0 y-ion bias 25.562675 0.889337 522.7741 1044.547 -0.0065 -6.3 -3.0999999999999996 8.76 3748.3 nuORFdb_human T345058_8:65675913-65676012:+ T345058_8:65675913-65676012:+ lincRNA lncRNA GN=nan lincRNA E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep1_Slot1-04_1_3496 40mio-rep2 E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep2_Slot2-5_1_3520.7121.1400.2 2 1 1 11.51 7.03 6.22 4.48 76.2 7 1 1 14400.0 Hydrogen 25 (L) 9 GTLTTKTHW (-) 9 ASLTTKYGGF G T/L/T|T/K/T|H\W 77.8 7 3 6 2 0 y-ion bias 24.605505 0.87606 522.7733 1044.547 -0.0085 -8.2 -3.299999999999999 8.76 3748.3 nuORFdb_human T345058_8:65675913-65676012:+ T345058_8:65675913-65676012:+ lincRNA lncRNA GN=nan lincRNA E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep2_Slot2-5_1_3520 40mio-rep3 E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep3_Slot2-5_1_3521.7139.1397.2 2 3 1 15.49 9.79 6.43 5.69 82.6 7 1 1 32600.0 Hydrogen 25 (L) 9 GTLTTKTHW (-) 9 ASLTTAPPTW G/T/L/T|T/K/T H\W 77.8 7 2 6 1 0 y-ion bias 24.663506 0.878022 522.7762 1044.547 -0.0025 -2.4 2.1999999999999997 8.76 3748.3 nuORFdb_human T345058_8:65675913-65676012:+ T345058_8:65675913-65676012:+ lincRNA lncRNA GN=nan lincRNA E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep3_Slot2-5_1_3521 40mio-rep4 E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep4_Slot2-5_1_3522.7144.1398.2 2 1 1 14.82 8.68 6.87 6.14 85.6 8 1 1 9080.0 Hydrogen 25 (L) 9 GTLTTKTHW (-) 9 ASLTTAPPTW G/T/L/T/T/K/T/H\W 100.0 9 1 7 0 0 y-ion bias 24.677412 0.87734 522.7745 1044.547 -0.0045 -4.4 0.2999999999999998 8.76 3748.3 nuORFdb_human T345058_8:65675913-65676012:+ T345058_8:65675913-65676012:+ lincRNA lncRNA GN=nan lincRNA E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep4_Slot2-5_1_3522 20mio-rep2 E_20221201_NO30_400nL_HLAc1_2e7_directIP_titration_rep3_Slot2-4_1_3518.14573.1460.2 2 1 1 10.19 8.11 2.01 2.08 77.5 5 1 1 7900.0 Hydrogen 38 (L) 9 GTRSVNLSW (F) 9 QVSRSGLSW G T R S\V/N\L\S\W 55.6 5 4 1 0 0 b-ion bias 50.336803 0.838668 510.2637 1019.527 -0.0061 -6.0 0.2999999999999998 9.75 6017.8 nuORFdb_human ENST00000550406.1_1_17:40282423-40282585:- ENST00000550406.1_1_17:40282423-40282585:- RAB5C 5' Overlap uORF 5' Overlap uORF GN=RAB5C 5' Overlap uORF E_20221201_NO30_400nL_HLAc1_2e7_directIP_titration_rep3_Slot2-4_1_3518 10mio-rep2 E_20221201_NO30_400nL_HLAc1_1e7_directIP_titration_rep2_Slot2-3_1_3513.12104.1553.2 2 3 1 9.09 2.21 8.16 6.88 70.2 7 0 1 16100.0 Hydrogen 6 (L) 9 GTSGTLRPW (R) 9 TGEKTRPW G T/S/G/T|L|R/P\W 77.8 7 3 6 2 0 y-ion bias 41.817993 0.77983 487.7526 974.505 -0.0067 -6.9 -0.20000000000000018 9.75 3371.9 smORF_v4|nuORFdb_human SMURF2_17:62658066-62658152:- SMURF2_17:62658066-62658152:-|ENST00000579692.1_1_17:29335545-29335704:+|ENST00000262435.13_1_17:62658063-62658153:- SMURF2 uORF 17:62658066-62658152:- 17 RP11-848P1.9 Pseudogene Pseudogene GN=SMURF2 E_20221201_NO30_400nL_HLAc1_1e7_directIP_titration_rep2_Slot2-3_1_3513 10mio-rep3 E_20221201_NO30_400nL_HLAc1_1e7_directIP_titration_rep3_Slot2-3_1_3514.12067.1534.2 2 4 1 12.03 4.95 7.62 7.08 86.2 8 0 1 15800.0 Hydrogen 6 (L) 9 GTSGTLRPW (R) 9 TGEKTRPW G/T/S/G/T/L|R/P\W 100.0 9 2 7 1 0 y-ion bias 41.690491 0.792075 487.7522 974.505 -0.0087 -8.9 -2.6000000000000005 9.75 3371.9 smORF_v4|nuORFdb_human SMURF2_17:62658066-62658152:- SMURF2_17:62658066-62658152:-|ENST00000579692.1_1_17:29335545-29335704:+|ENST00000262435.13_1_17:62658063-62658153:- SMURF2 uORF 17:62658066-62658152:- 17 RP11-848P1.9 Pseudogene Pseudogene GN=SMURF2 E_20221201_NO30_400nL_HLAc1_1e7_directIP_titration_rep3_Slot2-3_1_3514 20mio-rep1 E_20221201_NO30_400nL_HLAc1_2e7_directIP_titration_rep1_Slot2-4_1_3515.12011.1512.2 2 1 1 9.93 2.98 5.38 6.95 79.7 7 0 1 6300.0 Hydrogen 6 (L) 9 GTSGTLRPW (R) 9 GTSASRPLnA G/T/S/G/T|L/R/P W 77.8 7 1 7 1 0 y-ion bias 41.487026 0.805982 487.7526 974.505 -0.0067 -6.9 -0.7000000000000002 9.75 3371.9 smORF_v4|nuORFdb_human SMURF2_17:62658066-62658152:- SMURF2_17:62658066-62658152:-|ENST00000579692.1_1_17:29335545-29335704:+|ENST00000262435.13_1_17:62658063-62658153:- SMURF2 uORF 17:62658066-62658152:- 17 RP11-848P1.9 Pseudogene Pseudogene GN=SMURF2 E_20221201_NO30_400nL_HLAc1_2e7_directIP_titration_rep1_Slot2-4_1_3515 20mio-rep2 E_20221201_NO30_400nL_HLAc1_2e7_directIP_titration_rep3_Slot2-4_1_3518.12155.1516.2 2 4 1 11.41 3.01 7.09 8.39 79.9 8 0 1 21400.0 Hydrogen 6 (L) 9 GTSGTLRPW (R) 9 SAAAAARTAQG G/T/S/G/T|L/R/P\W 100.0 9 2 7 1 0 y-ion bias 41.985508 0.803255 487.7538 974.505 -0.0047 -4.8 1.5 9.75 3371.9 smORF_v4|nuORFdb_human SMURF2_17:62658066-62658152:- SMURF2_17:62658066-62658152:-|ENST00000579692.1_1_17:29335545-29335704:+|ENST00000262435.13_1_17:62658063-62658153:- SMURF2 uORF 17:62658066-62658152:- 17 RP11-848P1.9 Pseudogene Pseudogene GN=SMURF2 E_20221201_NO30_400nL_HLAc1_2e7_directIP_titration_rep3_Slot2-4_1_3518 20mio-rep3 E_20221201_NO30_400nL_HLAc1_2e7_directIP_titration_rep4_Slot2-4_1_3519.12049.1521.2 2 2 1 11.65 3.43 7.57 8.22 81.5 7 0 1 20500.0 Hydrogen 6 (L) 9 GTSGTLRPW (R) 9 ASSGGPRVFP G/T/S/G/T|L/R/P W 77.8 7 1 7 1 0 y-ion bias 41.6227 0.800179 487.7523 974.505 -0.0087 -8.9 -3.1000000000000005 9.75 3371.9 smORF_v4|nuORFdb_human SMURF2_17:62658066-62658152:- SMURF2_17:62658066-62658152:-|ENST00000579692.1_1_17:29335545-29335704:+|ENST00000262435.13_1_17:62658063-62658153:- SMURF2 uORF 17:62658066-62658152:- 17 RP11-848P1.9 Pseudogene Pseudogene GN=SMURF2 E_20221201_NO30_400nL_HLAc1_2e7_directIP_titration_rep4_Slot2-4_1_3519 40mio-rep1 E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep1_Slot1-04_1_3496.12321.1490.2 2 2 1 14.0 4.69 7.96 9.31 86.7 8 0 1 43500.0 Hydrogen 6 (L) 9 GTSGTLRPW (R) 9 GTSWRPITG G/T/S/G/T|L/R/P\W 100.0 9 2 7 1 0 y-ion bias 42.560997 0.820059 487.7546 974.505 -0.0027 -2.8 0.40000000000000036 9.75 3371.9 smORF_v4|nuORFdb_human SMURF2_17:62658066-62658152:- SMURF2_17:62658066-62658152:-|ENST00000579692.1_1_17:29335545-29335704:+|ENST00000262435.13_1_17:62658063-62658153:- SMURF2 uORF 17:62658066-62658152:- 17 RP11-848P1.9 Pseudogene Pseudogene GN=SMURF2 E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep1_Slot1-04_1_3496 40mio-rep2 E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep2_Slot2-5_1_3520.11994.1500.2 2 1 1 10.13 2.01 6.26 8.13 77.5 7 0 1 16300.0 Hydrogen 6 (L) 9 GTSGTLRPW (R) 9 SASGNAIQAR G T/S/G/T|L/R/P\W 77.8 7 2 6 1 0 y-ion bias 41.432182 0.813678 487.7535 974.505 -0.0047 -4.8 0.10000000000000053 9.75 3371.9 smORF_v4|nuORFdb_human SMURF2_17:62658066-62658152:- SMURF2_17:62658066-62658152:-|ENST00000579692.1_1_17:29335545-29335704:+|ENST00000262435.13_1_17:62658063-62658153:- SMURF2 uORF 17:62658066-62658152:- 17 RP11-848P1.9 Pseudogene Pseudogene GN=SMURF2 E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep2_Slot2-5_1_3520 40mio-rep3 E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep3_Slot2-5_1_3521.12005.1504.2 2 2 1 11.87 3.75 6.38 8.13 85.0 7 0 1 34300.0 Hydrogen 6 (L) 9 GTSGTLRPW (R) 9 SSGPGAGKRSA G/T/S/G/T|L/R/P W 77.8 7 1 7 1 0 y-ion bias 41.473526 0.810958 487.7537 974.505 -0.0047 -4.8 -0.20000000000000018 9.75 3371.9 smORF_v4|nuORFdb_human SMURF2_17:62658066-62658152:- SMURF2_17:62658066-62658152:-|ENST00000579692.1_1_17:29335545-29335704:+|ENST00000262435.13_1_17:62658063-62658153:- SMURF2 uORF 17:62658066-62658152:- 17 RP11-848P1.9 Pseudogene Pseudogene GN=SMURF2 E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep3_Slot2-5_1_3521 40mio-rep4 E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep4_Slot2-5_1_3522.11997.1500.2 2 1 1 8.98 2.95 5.56 6.03 77.0 6 0 1 11200.0 Hydrogen 6 (L) 9 GTSGTLRPW (R) 9 ASSGGPRVFP G T/S/G/T|L/R/P W 55.6 5 1 6 1 0 y-ion bias 41.430477 0.813335 487.7539 974.505 -0.0047 -4.8 -0.09999999999999964 9.75 3371.9 smORF_v4|nuORFdb_human SMURF2_17:62658066-62658152:- SMURF2_17:62658066-62658152:-|ENST00000579692.1_1_17:29335545-29335704:+|ENST00000262435.13_1_17:62658063-62658153:- SMURF2 uORF 17:62658066-62658152:- 17 RP11-848P1.9 Pseudogene Pseudogene GN=SMURF2 E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep4_Slot2-5_1_3522 5mio-rep1 E_20221201_NO30_400nL_HLAc1_5e6_directIP_titration_rep1_Slot1-37_1_3502.12393.1522.2 2 2 1 13.41 4.5 8.9 8.91 84.2 7 0 1 8840.0 Hydrogen 6 (L) 9 GTSGTLRPW (R) 9 SASGNAIQAR G T/S/G|T|L|R/P\W 77.8 7 4 6 3 0 dual b/y bias 42.809856 0.799745 487.7524 974.505 -0.0087 -8.9 -1.9000000000000004 9.75 3371.9 smORF_v4|nuORFdb_human SMURF2_17:62658066-62658152:- SMURF2_17:62658066-62658152:-|ENST00000579692.1_1_17:29335545-29335704:+|ENST00000262435.13_1_17:62658063-62658153:- SMURF2 uORF 17:62658066-62658152:- 17 RP11-848P1.9 Pseudogene Pseudogene GN=SMURF2 E_20221201_NO30_400nL_HLAc1_5e6_directIP_titration_rep1_Slot1-37_1_3502 10mio-rep1 E_20221201_NO30_400nL_HLAc1_1e7_directIP_titration_rep1_Slot2-3_1_3512.14213.677.1 1 2 1 11.3 6.68 3.5 4.62 83.0 7 0 0 4800.0 Hydrogen 16 (R) 9 GTVDAGPGW (I) 9 VSEGAGPWG G T/V/D/A|G|P\G\W 77.8 7 4 5 2 0 unbiased 49.100403 1.319942 859.387 859.394 -0.0075 -8.7 -0.9999999999999991 3.8 4723.3 nuORFdb_human ENST00000602285.1_1_5:176560902-176562162:+ ENST00000602285.1_1_5:176560902-176562162:+ NSD1 5' Overlap uORF 5' Overlap uORF GN=NSD1 5' Overlap uORF E_20221201_NO30_400nL_HLAc1_1e7_directIP_titration_rep1_Slot2-3_1_3512 10mio-rep2 E_20221201_NO30_400nL_HLAc1_1e7_directIP_titration_rep2_Slot2-3_1_3513.14188.673.1 1 1 1 10.26 7.6 2.88 2.66 83.1 6 0 0 2980.0 Hydrogen 16 (R) 9 GTVDAGPGW (I) 9 VSEGAGPWG G T V/D/A/G|P/G\W 66.7 6 2 5 1 0 y-ion bias 49.013855 1.322366 859.3876 859.394 -0.0065 -7.5 -0.7999999999999998 3.8 4723.3 nuORFdb_human ENST00000602285.1_1_5:176560902-176562162:+ ENST00000602285.1_1_5:176560902-176562162:+ NSD1 5' Overlap uORF 5' Overlap uORF GN=NSD1 5' Overlap uORF E_20221201_NO30_400nL_HLAc1_1e7_directIP_titration_rep2_Slot2-3_1_3513 10mio-rep3 E_20221201_NO30_400nL_HLAc1_1e7_directIP_titration_rep3_Slot2-3_1_3514.14130.679.1 1 1 1 9.69 5.77 4.64 3.92 80.3 6 0 0 3900.0 Hydrogen 16 (R) 9 GTVDAGPGW (I) 9 VSEGAGPWG G T/V/D/A/G|P G\W 55.6 5 2 5 1 0 y-ion bias 48.815716 1.318867 859.3891 859.394 -0.0055 -6.4 -0.10000000000000053 3.8 4723.3 nuORFdb_human ENST00000602285.1_1_5:176560902-176562162:+ ENST00000602285.1_1_5:176560902-176562162:+ NSD1 5' Overlap uORF 5' Overlap uORF GN=NSD1 5' Overlap uORF E_20221201_NO30_400nL_HLAc1_1e7_directIP_titration_rep3_Slot2-3_1_3514 20mio-rep1 E_20221201_NO30_400nL_HLAc1_2e7_directIP_titration_rep1_Slot2-4_1_3515.14108.675.1 1 1 1 11.27 7.79 2.71 3.48 81.8 7 0 0 3910.0 Hydrogen 16 (R) 9 GTVDAGPGW (I) 9 VSEGAGPWG G T/V/D/A|G|P\G\W 77.8 7 4 5 2 0 unbiased 48.72691 1.321165 859.3903 859.394 -0.0045 -5.2 1.0 3.8 4723.3 nuORFdb_human ENST00000602285.1_1_5:176560902-176562162:+ ENST00000602285.1_1_5:176560902-176562162:+ NSD1 5' Overlap uORF 5' Overlap uORF GN=NSD1 5' Overlap uORF E_20221201_NO30_400nL_HLAc1_2e7_directIP_titration_rep1_Slot2-4_1_3515 20mio-rep2 E_20221201_NO30_400nL_HLAc1_2e7_directIP_titration_rep3_Slot2-4_1_3518.14237.668.1 1 2 1 12.47 7.11 3.88 5.36 86.4 7 0 0 16200.0 Hydrogen 16 (R) 9 GTVDAGPGW (I) 9 VSEGAGPWG G T/V/D/A|G|P|G\W 77.8 7 4 6 3 0 dual b/y bias 49.177097 1.325761 859.3904 859.394 -0.0045 -5.2 1.0999999999999996 3.8 4723.3 nuORFdb_human ENST00000602285.1_1_5:176560902-176562162:+ ENST00000602285.1_1_5:176560902-176562162:+ NSD1 5' Overlap uORF 5' Overlap uORF GN=NSD1 5' Overlap uORF E_20221201_NO30_400nL_HLAc1_2e7_directIP_titration_rep3_Slot2-4_1_3518 20mio-rep3 E_20221201_NO30_400nL_HLAc1_2e7_directIP_titration_rep4_Slot2-4_1_3519.14128.666.1 1 1 1 12.87 9.26 3.38 3.61 84.9 7 0 0 5750.0 Hydrogen 16 (R) 9 GTVDAGPGW (I) 9 VSEGAGPWG G T/V/D|A|G|P\G\W 77.8 7 5 5 3 0 dual b/y bias 48.799751 1.326501 859.3906 859.394 -0.0035 -4.0 1.7999999999999998 3.8 4723.3 nuORFdb_human ENST00000602285.1_1_5:176560902-176562162:+ ENST00000602285.1_1_5:176560902-176562162:+ NSD1 5' Overlap uORF 5' Overlap uORF GN=NSD1 5' Overlap uORF E_20221201_NO30_400nL_HLAc1_2e7_directIP_titration_rep4_Slot2-4_1_3519 40mio-rep1 E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep1_Slot1-04_1_3496.14434.651.1 1 2 1 13.41 8.79 4.06 4.62 86.1 7 0 0 38100.0 Hydrogen 16 (R) 9 GTVDAGPGW (I) 9 VSEGAGPWG G T/V/D|A/G|P|G\W 77.8 7 4 6 3 0 dual b/y bias 49.85968 1.335589 859.3914 859.394 -0.0035 -4.0 -0.7999999999999998 3.8 4723.3 nuORFdb_human ENST00000602285.1_1_5:176560902-176562162:+ ENST00000602285.1_1_5:176560902-176562162:+ NSD1 5' Overlap uORF 5' Overlap uORF GN=NSD1 5' Overlap uORF E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep1_Slot1-04_1_3496 40mio-rep2 E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep2_Slot2-5_1_3520.14104.665.1 1 2 1 11.36 7.36 3.39 4.0 82.2 7 0 0 29800.0 Hydrogen 16 (R) 9 GTVDAGPGW (I) 9 VSEGAGPWG G T/V/D/A|G|P|G\W 77.8 7 4 6 3 0 dual b/y bias 48.721428 1.32752 859.3889 859.394 -0.0055 -6.4 -1.5 3.8 4723.3 nuORFdb_human ENST00000602285.1_1_5:176560902-176562162:+ ENST00000602285.1_1_5:176560902-176562162:+ NSD1 5' Overlap uORF 5' Overlap uORF GN=NSD1 5' Overlap uORF E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep2_Slot2-5_1_3520 40mio-rep3 E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep3_Slot2-5_1_3521.14095.663.1 1 1 1 12.94 8.38 2.67 4.56 85.2 7 0 0 6910.0 Hydrogen 16 (R) 9 GTVDAGPGW (I) 9 VSEGAGPWG G T/V/D|A|G|P\G\W 77.8 7 5 5 3 0 dual b/y bias 48.693264 1.328222 859.3901 859.394 -0.0045 -5.2 -0.6000000000000005 3.8 4723.3 nuORFdb_human ENST00000602285.1_1_5:176560902-176562162:+ ENST00000602285.1_1_5:176560902-176562162:+ NSD1 5' Overlap uORF 5' Overlap uORF GN=NSD1 5' Overlap uORF E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep3_Slot2-5_1_3521 40mio-rep4 E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep4_Slot2-5_1_3522.14074.658.1 1 2 1 11.78 7.96 3.77 3.82 82.6 7 0 0 27000.0 Hydrogen 16 (R) 9 GTVDAGPGW (I) 9 VSEGAGPWG G T/V/D/A|G|P\G\W 77.8 7 4 5 2 0 unbiased 48.604179 1.331434 859.39 859.394 -0.0045 -5.2 -0.5 3.8 4723.3 nuORFdb_human ENST00000602285.1_1_5:176560902-176562162:+ ENST00000602285.1_1_5:176560902-176562162:+ NSD1 5' Overlap uORF 5' Overlap uORF GN=NSD1 5' Overlap uORF E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep4_Slot2-5_1_3522 5mio-rep2 E_20221201_NO30_400nL_HLAc1_5e6_directIP_titration_rep2_Slot2-2_1_3510.14389.690.1 1 2 1 8.4 4.23 2.05 4.17 76.4 7 0 0 3870.0 Hydrogen 16 (R) 9 GTVDAGPGW (I) 9 VSEGAGPWG G T/V/D/A/G|P\G\W 77.8 7 3 5 1 0 unbiased 49.699471 1.312211 859.3832 859.394 -0.0115 -13.3 -4.600000000000001 3.8 4723.3 nuORFdb_human ENST00000602285.1_1_5:176560902-176562162:+ ENST00000602285.1_1_5:176560902-176562162:+ NSD1 5' Overlap uORF 5' Overlap uORF GN=NSD1 5' Overlap uORF E_20221201_NO30_400nL_HLAc1_5e6_directIP_titration_rep2_Slot2-2_1_3510 5mio-rep3 E_20221201_NO30_400nL_HLAc1_5e6_directIP_titration_rep3_Slot2-2_1_3511.14420.683.1 1 1 1 11.05 6.2 4.16 4.84 80.4 7 0 0 2630.0 Hydrogen 16 (R) 9 GTVDAGPGW (I) 9 VSEGAGPWG G T/V/D/A|G|P\G\W 77.8 7 4 5 2 0 unbiased 49.811272 1.316415 859.3882 859.394 -0.0065 -7.5 0.6999999999999993 3.8 4723.3 nuORFdb_human ENST00000602285.1_1_5:176560902-176562162:+ ENST00000602285.1_1_5:176560902-176562162:+ NSD1 5' Overlap uORF 5' Overlap uORF GN=NSD1 5' Overlap uORF E_20221201_NO30_400nL_HLAc1_5e6_directIP_titration_rep3_Slot2-2_1_3511 40mio-rep1 E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep1_Slot1-04_1_3496.11740.1334.2 2 1 1 9.67 2.52 5.94 7.15 67.7 7 1 1 11800.0 Hydrogen 26 (C) 9 GTWIIHRTY (L) 9 SAWLCLQLR G/T/W|I|I H|R/T\Y 77.8 7 4 6 3 0 dual b/y bias 40.5546 0.917685 573.8049 1146.605 -0.0027 -2.4 0.8000000000000003 8.75 6717.8 nuORFdb_human ENST00000368990.7_2_10:124152762-124159877:+ ENST00000368990.7_2_10:124152762-124159877:+ PLEKHA1 Out-of-Frame Out-of-Frame GN=PLEKHA1 Out-of-Frame E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep1_Slot1-04_1_3496 1mio-rep1 E_20221201_NO30_400nL_HLAc1_1e6_directIP_titration_rep1_Slot1-06_1_3498.12208.461.1 1 3 1 12.18 9.83 12.18 83.8 7 0 0 5680.0 Hydrogen 4 (S) 9 GTYSGNTDW (A) 9 G T/Y/S|G/N|T\D\W 77.8 7 4 5 2 0 unbiased 42.16988 1.44903 1000.3996 1000.401 -0.0007 -0.7 4.2 3.8 2134.3 nuORFdb_human ENST00000590760.5_1_17:40729310-40729522:- ENST00000590760.5_1_17:40729310-40729522:- PSMC3IP 5' uORF 5' uORF GN=PSMC3IP 5' uORF E_20221201_NO30_400nL_HLAc1_1e6_directIP_titration_rep1_Slot1-06_1_3498 10mio-rep1 E_20221201_NO30_400nL_HLAc1_1e7_directIP_titration_rep1_Slot2-3_1_3512.11862.486.1 1 2 1 14.3 1.08 10.1 13.23 86.3 7 0 0 8990.0 Hydrogen 4 (S) 9 GTYSGNTDW (A) 9 ASYSAGDSGDA G T/Y/S/G|N|T|D\W 77.8 7 4 6 3 0 dual b/y bias 40.982113 1.434517 1000.3945 1000.401 -0.0107 -10.7 -2.999999999999999 3.8 2134.3 nuORFdb_human ENST00000590760.5_1_17:40729310-40729522:- ENST00000590760.5_1_17:40729310-40729522:- PSMC3IP 5' uORF 5' uORF GN=PSMC3IP 5' uORF E_20221201_NO30_400nL_HLAc1_1e7_directIP_titration_rep1_Slot2-3_1_3512 10mio-rep2 E_20221201_NO30_400nL_HLAc1_1e7_directIP_titration_rep2_Slot2-3_1_3513.11840.482.1 1 5 1 15.09 0.22 9.42 14.87 88.1 7 0 0 13300.0 Hydrogen 4 (S) 9 GTYSGNTDW (A) 9 mGKSCESW G T/Y/S|G/N|T|D\W 77.8 7 4 6 3 0 dual b/y bias 40.904057 1.436819 1000.3934 1000.401 -0.0107 -10.7 -3.999999999999999 3.8 2134.3 nuORFdb_human ENST00000590760.5_1_17:40729310-40729522:- ENST00000590760.5_1_17:40729310-40729522:- PSMC3IP 5' uORF 5' uORF GN=PSMC3IP 5' uORF E_20221201_NO30_400nL_HLAc1_1e7_directIP_titration_rep2_Slot2-3_1_3513 10mio-rep3 E_20221201_NO30_400nL_HLAc1_1e7_directIP_titration_rep3_Slot2-3_1_3514.11812.488.1 1 3 1 15.36 2.32 10.85 13.04 87.4 7 0 0 22100.0 Hydrogen 4 (S) 9 GTYSGNTDW (A) 9 SAEYESEW G T/Y/S|G|N|T\D\W 77.8 7 5 5 3 0 dual b/y bias 40.810368 1.433037 1000.3919 1000.401 -0.0107 -10.7 -4.3999999999999995 3.8 2134.3 nuORFdb_human ENST00000590760.5_1_17:40729310-40729522:- ENST00000590760.5_1_17:40729310-40729522:- PSMC3IP 5' uORF 5' uORF GN=PSMC3IP 5' uORF E_20221201_NO30_400nL_HLAc1_1e7_directIP_titration_rep3_Slot2-3_1_3514 20mio-rep1 E_20221201_NO30_400nL_HLAc1_2e7_directIP_titration_rep1_Slot2-4_1_3515.11772.486.1 1 3 1 13.99 10.55 13.99 86.7 7 0 0 33800.0 Hydrogen 4 (S) 9 GTYSGNTDW (A) 9 G T/Y/S|G|N|T-D\W 77.8 7 4 5 3 1 dual b/y bias 40.662144 1.434371 1000.3942 1000.401 -0.0107 -10.7 -4.499999999999999 3.8 2134.3 nuORFdb_human ENST00000590760.5_1_17:40729310-40729522:- ENST00000590760.5_1_17:40729310-40729522:- PSMC3IP 5' uORF 5' uORF GN=PSMC3IP 5' uORF E_20221201_NO30_400nL_HLAc1_2e7_directIP_titration_rep1_Slot2-4_1_3515 20mio-rep3 E_20221201_NO30_400nL_HLAc1_2e7_directIP_titration_rep4_Slot2-4_1_3519.11782.470.1 1 4 1 15.1 0.94 11.0 14.16 87.5 7 0 0 54000.0 Hydrogen 4 (S) 9 GTYSGNTDW (A) 9 SFSSADNLC G T/Y/S|G|N|T\D\W 77.8 7 5 5 3 0 dual b/y bias 40.70076 1.444003 1000.386 1000.401 -0.0107 -10.7 -4.8999999999999995 3.8 2134.3 nuORFdb_human ENST00000590760.5_1_17:40729310-40729522:- ENST00000590760.5_1_17:40729310-40729522:- PSMC3IP 5' uORF 5' uORF GN=PSMC3IP 5' uORF E_20221201_NO30_400nL_HLAc1_2e7_directIP_titration_rep4_Slot2-4_1_3519 40mio-rep1 E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep1_Slot1-04_1_3496.12102.458.1 1 5 1 14.49 -0.03 10.87 14.51 87.1 7 0 0 249000.0 Hydrogen 4 (S) 9 GTYSGNTDW (A) 9 FSSMDLDDA G T/Y/S|G/N|T\D\W 77.8 7 4 5 2 0 unbiased 41.804928 1.451037 1000.4001 1000.401 -0.0007 -0.7 2.5 3.8 2134.3 nuORFdb_human ENST00000590760.5_1_17:40729310-40729522:- ENST00000590760.5_1_17:40729310-40729522:- PSMC3IP 5' uORF 5' uORF GN=PSMC3IP 5' uORF E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep1_Slot1-04_1_3496 40mio-rep2 E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep2_Slot2-5_1_3520.11762.476.1 1 4 1 15.07 0.77 9.28 14.3 89.5 7 0 0 103000.0 Hydrogen 4 (S) 9 GTYSGNTDW (A) 9 mGKSCESW G T/Y/S|G/N|T|D\W 77.8 7 4 6 3 0 dual b/y bias 40.631424 1.440116 1000.3971 1000.401 -0.0007 -0.7 4.2 3.8 2134.3 nuORFdb_human ENST00000590760.5_1_17:40729310-40729522:- ENST00000590760.5_1_17:40729310-40729522:- PSMC3IP 5' uORF 5' uORF GN=PSMC3IP 5' uORF E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep2_Slot2-5_1_3520 40mio-rep3 E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep3_Slot2-5_1_3521.11752.479.1 1 4 1 14.76 0.73 9.7 14.03 88.3 7 0 0 76700.0 Hydrogen 4 (S) 9 GTYSGNTDW (A) 9 TDGNFDSASS G T/Y/S|G/N|T/D\W 77.8 7 3 6 2 0 y-ion bias 40.599159 1.438638 1000.3969 1000.401 -0.0007 -0.7 3.8999999999999995 3.8 2134.3 nuORFdb_human ENST00000590760.5_1_17:40729310-40729522:- ENST00000590760.5_1_17:40729310-40729522:- PSMC3IP 5' uORF 5' uORF GN=PSMC3IP 5' uORF E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep3_Slot2-5_1_3521 40mio-rep4 E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep4_Slot2-5_1_3522.11765.1493.2 2 3 1 16.0 3.43 10.38 12.56 91.6 7 0 0 91500.0 Hydrogen 4 (S) 9 GTYSGNTDW (A) 9 SAEYESEW G T/Y/S|G|N|T/D\W 77.8 7 4 6 3 0 dual b/y bias 40.629749 0.817823 500.7016 1000.401 -0.0039 -3.9 0.8000000000000003 3.8 2134.3 nuORFdb_human ENST00000590760.5_1_17:40729310-40729522:- ENST00000590760.5_1_17:40729310-40729522:- PSMC3IP 5' uORF 5' uORF GN=PSMC3IP 5' uORF E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep4_Slot2-5_1_3522 5mio-rep1 E_20221201_NO30_400nL_HLAc1_5e6_directIP_titration_rep1_Slot1-37_1_3502.12079.473.1 1 2 1 13.25 0.01 9.86 13.24 83.8 7 0 0 7270.0 Hydrogen 4 (S) 9 GTYSGNTDW (A) 9 FGDGmIETD G T/Y/S|G/N|T|D\W 77.8 7 4 6 3 0 dual b/y bias 41.725285 1.441968 1000.3882 1000.401 -0.0107 -10.7 -3.6999999999999993 3.8 2134.3 nuORFdb_human ENST00000590760.5_1_17:40729310-40729522:- ENST00000590760.5_1_17:40729310-40729522:- PSMC3IP 5' uORF 5' uORF GN=PSMC3IP 5' uORF E_20221201_NO30_400nL_HLAc1_5e6_directIP_titration_rep1_Slot1-37_1_3502 5mio-rep2 E_20221201_NO30_400nL_HLAc1_5e6_directIP_titration_rep2_Slot2-2_1_3510.12061.491.1 1 2 1 13.5 1.38 10.07 12.12 83.8 7 0 0 5800.0 Hydrogen 4 (S) 9 GTYSGNTDW (A) 9 ASTMSAGGDFG G T/Y/S/G/N|T|D\W 77.8 7 3 6 2 0 y-ion bias 41.659042 1.431113 1000.3949 1000.401 -0.0107 -10.7 -2.0 3.8 2134.3 nuORFdb_human ENST00000590760.5_1_17:40729310-40729522:- ENST00000590760.5_1_17:40729310-40729522:- PSMC3IP 5' uORF 5' uORF GN=PSMC3IP 5' uORF E_20221201_NO30_400nL_HLAc1_5e6_directIP_titration_rep2_Slot2-2_1_3510 5mio-rep3 E_20221201_NO30_400nL_HLAc1_5e6_directIP_titration_rep3_Slot2-2_1_3511.12078.491.1 1 1 1 11.7 3.04 10.12 8.66 81.7 6 0 0 2120.0 Hydrogen 4 (S) 9 GTYSGNTDW (A) 9 SAEYESEW G T/Y/S|G/N|T D\W 55.6 5 3 5 2 0 unbiased 41.724747 1.431545 1000.3895 1000.401 -0.0107 -10.7 -2.5 3.8 2134.3 nuORFdb_human ENST00000590760.5_1_17:40729310-40729522:- ENST00000590760.5_1_17:40729310-40729522:- PSMC3IP 5' uORF 5' uORF GN=PSMC3IP 5' uORF E_20221201_NO30_400nL_HLAc1_5e6_directIP_titration_rep3_Slot2-2_1_3511 40mio-rep1 E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep1_Slot1-04_1_3496.6776.1446.2 2 1 1 10.47 9.43 2.6 1.04 78.9 5 0 0 6600.0 Hydrogen 23 (L) 11 GVAGGPQQEVL (F) 11 GPGQGLGQEVL G V A G G P\Q|Q|E\V\L 45.5 5 5 2 2 0 b-ion bias 23.414921 0.847614 527.7777 1054.553 -0.004 -3.8 -0.5999999999999996 4.0 4078.6 nuORFdb_human ENST00000593779.1_1_19:16698433-16698541:- ENST00000593779.1_1_19:16698433-16698541:- CTD-3222D19.5 ncRNA Retained Intron lncRNA GN=CTD-3222D19.5 ncRNA Retained Intron E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep1_Slot1-04_1_3496 40mio-rep1 E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep1_Slot1-04_1_3496.22928.361.1 1 3 1 10.25 4.83 4.4 5.42 77.7 5 0 0 14300.0 Hydrogen 65 (G) 10 GVFEETGSPW (-) 10 MMVAETGSPW G V F E|E|T\G\S|P W 40.0 4 5 3 3 0 unbiased 79.206932 1.508376 1108.4903 1108.495 -0.0046 -4.1 -0.8999999999999995 3.8 7704.7 nuORFdb_human ENST00000479665.5_2_3:28390190-28390412:- ENST00000479665.5_2_3:28390190-28390412:- AZI2 5' uORF 5' uORF GN=AZI2 5' uORF E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep1_Slot1-04_1_3496 40mio-rep3 E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep3_Slot2-5_1_3521.22682.380.1 1 1 1 8.95 5.59 2.43 3.35 69.3 5 0 0 3870.0 Hydrogen 65 (G) 10 GVFEETGSPW (-) 10 MTSELASSPW G V F E/E\T\G|S|P W 40.0 4 4 3 2 0 unbiased 78.353539 1.497264 1108.4856 1108.495 -0.0046 -4.1 0.5 3.8 7704.7 nuORFdb_human ENST00000479665.5_2_3:28390190-28390412:- ENST00000479665.5_2_3:28390190-28390412:- AZI2 5' uORF 5' uORF GN=AZI2 5' uORF E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep3_Slot2-5_1_3521 40mio-rep4 E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep4_Slot2-5_1_3522.22687.375.1 1 1 1 8.54 5.75 2.73 2.8 72.2 5 0 0 4470.0 Hydrogen 65 (G) 10 GVFEETGSPW (-) 10 MMVAETGSPW G V F E/E/T\G|S|P W 40.0 4 3 4 2 0 unbiased 78.359215 1.5001 1108.4904 1108.495 -0.0046 -4.1 0.6000000000000005 3.8 7704.7 nuORFdb_human ENST00000479665.5_2_3:28390190-28390412:- ENST00000479665.5_2_3:28390190-28390412:- AZI2 5' uORF 5' uORF GN=AZI2 5' uORF E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep4_Slot2-5_1_3522 40mio-rep1 E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep1_Slot1-04_1_3496.15134.1378.2 2 1 1 10.39 7.98 3.72 2.41 82.2 6 1 1 10100.0 Hydrogen 23 (V) 9 GVFPYQREY (E) 9 VGnRSDQPER G V/F|P/Y/Q/R E\Y 55.6 5 2 5 1 0 y-ion bias 52.277027 0.890333 579.7811 1158.558 -0.0031 -2.7 0.5 6.0 6601.2 nuORFdb_human ENST00000553891.5_1_14:73491136-73491307:- ENST00000553891.5_1_14:73491136-73491307:- ZFYVE1 5' Overlap uORF 5' Overlap uORF GN=ZFYVE1 5' Overlap uORF E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep1_Slot1-04_1_3496 40mio-rep2 E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep2_Slot2-5_1_3520.14795.1387.2 2 1 1 9.46 6.94 2.69 2.52 85.3 6 1 1 7050.0 Hydrogen 23 (V) 9 GVFPYQREY (E) 9 LALGNECPER G V/F|P/Y/Q|R E-Y 55.6 5 2 5 2 1 y-ion bias 51.107834 0.884749 579.7811 1158.558 -0.0031 -2.7 2.2 6.0 6601.2 nuORFdb_human ENST00000553891.5_1_14:73491136-73491307:- ENST00000553891.5_1_14:73491136-73491307:- ZFYVE1 5' Overlap uORF 5' Overlap uORF GN=ZFYVE1 5' Overlap uORF E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep2_Slot2-5_1_3520 40mio-rep4 E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep4_Slot2-5_1_3522.14818.1393.2 2 2 1 10.3 7.07 3.72 3.22 82.0 6 1 1 10800.0 Hydrogen 23 (V) 9 GVFPYQREY (E) 9 YRVGDTYER G V/F|P/Y/Q|R E\Y 55.6 5 3 5 2 0 unbiased 51.175293 0.880686 579.7814 1158.558 -0.0031 -2.7 2.0 6.0 6601.2 nuORFdb_human ENST00000553891.5_1_14:73491136-73491307:- ENST00000553891.5_1_14:73491136-73491307:- ZFYVE1 5' Overlap uORF 5' Overlap uORF GN=ZFYVE1 5' Overlap uORF E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep4_Slot2-5_1_3522 40mio-rep1 E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep1_Slot1-04_1_3496.14976.1479.2 2 2 1 13.41 4.35 8.45 9.05 84.1 7 1 1 30800.0 Hydrogen 5 (C) 9 GVMENLSRV (-) 9 RMELmPLV G V/M/E|N/L|S\R\V 77.8 7 4 5 2 0 unbiased 51.731544 0.826611 502.7625 1004.519 -0.0006 -0.6 2.6 6.0 1577.9 nuORFdb_human ENST00000565534.5_1_15:75658926-75659886:- ENST00000565534.5_1_15:75658926-75659886:- MAN2C1 ncRNA Retained Intron lncRNA GN=MAN2C1 ncRNA Retained Intron E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep1_Slot1-04_1_3496 40mio-rep2 E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep2_Slot2-5_1_3520.14604.1482.2 2 1 1 9.57 3.59 7.24 5.98 67.6 7 1 1 7010.0 Hydrogen 5 (C) 9 GVMENLSRV (-) 9 mNLGKEVR G V/M|E|N/L/S\R\V 77.8 7 4 5 2 0 unbiased 50.448433 0.825045 502.7633 1004.519 -0.0006 -0.6 4.300000000000001 6.0 1577.9 nuORFdb_human ENST00000565534.5_1_15:75658926-75659886:- ENST00000565534.5_1_15:75658926-75659886:- MAN2C1 ncRNA Retained Intron lncRNA GN=MAN2C1 ncRNA Retained Intron E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep2_Slot2-5_1_3520 40mio-rep3 E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep3_Slot2-5_1_3521.14615.1489.2 2 1 1 10.06 4.15 7.34 5.91 70.9 6 1 1 6960.0 Hydrogen 5 (C) 9 GVMENLSRV (-) 9 VGMEnKVVQ G V/M|E|N/L|S R\V 55.6 5 4 5 3 0 dual b/y bias 50.488567 0.820223 502.7632 1004.519 -0.0006 -0.6 3.9999999999999996 6.0 1577.9 nuORFdb_human ENST00000565534.5_1_15:75658926-75659886:- ENST00000565534.5_1_15:75658926-75659886:- MAN2C1 ncRNA Retained Intron lncRNA GN=MAN2C1 ncRNA Retained Intron E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep3_Slot2-5_1_3521 20mio-rep1 E_20221201_NO30_400nL_HLAc1_2e7_directIP_titration_rep1_Slot2-4_1_3515.9185.1486.2 2 2 1 16.83 6.92 11.49 9.91 76.7 8 1 1 12400.0 M7m Hydrogen 5 (C) 9 GVmENLSRV (-) 9 VGmALGAADTV G/V/m|E|N|L|S|R\V 100.0 9 6 7 5 0 dual b/y bias m:Oxidized methionine 31.728001 0.82239 510.757 1004.519 15.9874 -7.4 -1.2000000000000002 6.0 1577.9 nuORFdb_human ENST00000565534.5_1_15:75658926-75659886:- ENST00000565534.5_1_15:75658926-75659886:- MAN2C1 ncRNA Retained Intron lncRNA GN=MAN2C1 ncRNA Retained Intron E_20221201_NO30_400nL_HLAc1_2e7_directIP_titration_rep1_Slot2-4_1_3515 20mio-rep2 E_20221201_NO30_400nL_HLAc1_2e7_directIP_titration_rep3_Slot2-4_1_3518.9362.1489.2 2 1 1 7.78 2.5 5.92 5.28 57.2 6 1 1 5250.0 M7m Hydrogen 5 (C) 9 GVmENLSRV (-) 9 VGmEnKVVQ G V/m|E N/L|S\R\V 55.6 5 4 4 2 0 unbiased m:Oxidized methionine 32.340511 0.820246 510.7562 1004.519 15.9854 -9.3 -3.000000000000001 6.0 1577.9 nuORFdb_human ENST00000565534.5_1_15:75658926-75659886:- ENST00000565534.5_1_15:75658926-75659886:- MAN2C1 ncRNA Retained Intron lncRNA GN=MAN2C1 ncRNA Retained Intron E_20221201_NO30_400nL_HLAc1_2e7_directIP_titration_rep3_Slot2-4_1_3518 20mio-rep3 E_20221201_NO30_400nL_HLAc1_2e7_directIP_titration_rep4_Slot2-4_1_3519.9224.1484.2 2 1 1 13.42 8.11 4.94 5.31 73.5 7 1 1 6270.0 M7m Hydrogen 5 (C) 9 GVmENLSRV (-) 9 VGmEnKVVQ G/V/m|E\N|L|S R\V 77.8 7 5 5 3 0 dual b/y bias m:Oxidized methionine 31.865368 0.82329 510.7588 1004.519 15.9914 -3.5 2.3 6.0 1577.9 nuORFdb_human ENST00000565534.5_1_15:75658926-75659886:- ENST00000565534.5_1_15:75658926-75659886:- MAN2C1 ncRNA Retained Intron lncRNA GN=MAN2C1 ncRNA Retained Intron E_20221201_NO30_400nL_HLAc1_2e7_directIP_titration_rep4_Slot2-4_1_3519 40mio-rep1 E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep1_Slot1-04_1_3496.9509.1456.2 2 1 1 12.09 6.18 6.09 5.9 71.8 7 1 1 9690.0 M7m Hydrogen 5 (C) 9 GVmENLSRV (-) 9 VGmEnKVVQ G/V/m|E\N/L|S R\V 77.8 7 4 5 2 0 unbiased m:Oxidized methionine 32.850872 0.840974 510.7599 1004.519 15.9934 -1.5 1.7000000000000002 6.0 1577.9 nuORFdb_human ENST00000565534.5_1_15:75658926-75659886:- ENST00000565534.5_1_15:75658926-75659886:- MAN2C1 ncRNA Retained Intron lncRNA GN=MAN2C1 ncRNA Retained Intron E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep1_Slot1-04_1_3496 40mio-rep2 E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep2_Slot2-5_1_3520.9201.1479.2 2 1 1 14.87 9.01 7.32 5.86 82.2 7 1 1 9660.0 M7m Hydrogen 5 (C) 9 GVmENLSRV (-) 9 VGmEnKVVQ G V/m|E|N/L\S|R\V 77.8 7 5 5 3 0 dual b/y bias m:Oxidized methionine 31.787224 0.82658 510.757 1004.519 15.9874 -7.4 -2.5 6.0 1577.9 nuORFdb_human ENST00000565534.5_1_15:75658926-75659886:- ENST00000565534.5_1_15:75658926-75659886:- MAN2C1 ncRNA Retained Intron lncRNA GN=MAN2C1 ncRNA Retained Intron E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep2_Slot2-5_1_3520 40mio-rep3 E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep3_Slot2-5_1_3521.9196.1476.2 2 1 1 16.07 7.31 9.87 8.76 86.3 8 1 1 7900.0 M7m Hydrogen 5 (C) 9 GVmENLSRV (-) 9 VGmALGAADTV G/V/m|E/N/L|S|R\V 100.0 9 4 7 3 0 dual b/y bias m:Oxidized methionine 31.77084 0.828789 510.7564 1004.519 15.9854 -9.3 -4.700000000000001 6.0 1577.9 nuORFdb_human ENST00000565534.5_1_15:75658926-75659886:- ENST00000565534.5_1_15:75658926-75659886:- MAN2C1 ncRNA Retained Intron lncRNA GN=MAN2C1 ncRNA Retained Intron E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep3_Slot2-5_1_3521 40mio-rep4 E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep4_Slot2-5_1_3522.9208.1478.2 2 1 1 17.71 8.46 10.31 9.24 94.1 7 1 1 6890.0 M7m Hydrogen 5 (C) 9 GVmENLSRV (-) 9 RmELmPLV G V/m|E|N|L|S|R\V 77.8 7 6 6 5 0 dual b/y bias m:Oxidized methionine 31.802608 0.827234 510.7583 1004.519 15.9894 -5.4 -0.7000000000000002 6.0 1577.9 nuORFdb_human ENST00000565534.5_1_15:75658926-75659886:- ENST00000565534.5_1_15:75658926-75659886:- MAN2C1 ncRNA Retained Intron lncRNA GN=MAN2C1 ncRNA Retained Intron E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep4_Slot2-5_1_3522 40mio-rep1 E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep1_Slot1-04_1_3496.12600.1577.3 3 2 1 9.93 3.22 4.6 6.71 60.2 7 1 1 11900.0 Hydrogen 22 (K) 9 HALQFMKHW (I) 9 HSTNLFRHW H/A L\Q|F|M/K|H\W 77.8 7 5 5 3 0 dual b/y bias 43.525032 0.764847 399.8686 1197.599 -0.0061 -5.1 -1.8999999999999995 8.76 5468.6 smORF_v4|nuORFdb_human FLJ46906_6:139013732-139015647:+ FLJ46906_6:139013732-139015647:+|ENST00000432673.1_1_6:139013686-139015644:+|ENST00000428044.5_1_6:139015455-139015644:+ FLJ46906 lncRNA 6:139013732-139015647:+ 6 RP11-390P2.4 Antisense lncRNA GN=FLJ46906 E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep1_Slot1-04_1_3496 1mio-rep1 E_20221201_NO30_400nL_HLAc1_1e6_directIP_titration_rep1_Slot1-06_1_3498.18533.1498.2 2 1 1 10.08 7.49 2.32 2.59 88.5 5 0 0 2950.0 Hydrogen 24 (G) 9 HEGVATVIW (-) 9 PDPREVLW H E G V\A\T\V|I\W 55.6 5 5 1 1 0 b-ion bias 64.006485 0.814712 506.2649 1011.526 -0.0031 -3.0 1.9000000000000004 5.24 3623.1 nuORFdb_human ENST00000552593.1_1_12:53575584-53575683:+ ENST00000552593.1_1_12:53575584-53575683:+ ZNF740 ncRNA Retained Intron lncRNA GN=ZNF740 ncRNA Retained Intron E_20221201_NO30_400nL_HLAc1_1e6_directIP_titration_rep1_Slot1-06_1_3498 10mio-rep2 E_20221201_NO30_400nL_HLAc1_1e7_directIP_titration_rep2_Slot2-3_1_3513.18219.1503.2 2 3 1 12.68 10.02 2.66 2.66 90.8 5 0 0 15900.0 Hydrogen 24 (G) 9 HEGVATVIW (-) 9 QLSEHVIW H E G V|A\T|V|I\W 55.6 5 5 3 3 0 dual b/y bias 62.933285 0.811543 506.2629 1011.526 -0.0071 -7.0 -0.2999999999999998 5.24 3623.1 nuORFdb_human ENST00000552593.1_1_12:53575584-53575683:+ ENST00000552593.1_1_12:53575584-53575683:+ ZNF740 ncRNA Retained Intron lncRNA GN=ZNF740 ncRNA Retained Intron E_20221201_NO30_400nL_HLAc1_1e7_directIP_titration_rep2_Slot2-3_1_3513 10mio-rep3 E_20221201_NO30_400nL_HLAc1_1e7_directIP_titration_rep3_Slot2-3_1_3514.18171.1510.2 2 2 1 12.84 9.75 3.09 3.09 94.6 5 0 0 10100.0 Hydrogen 24 (G) 9 HEGVATVIW (-) 9 PDPREVLW H E G V\A|T|V|I\W 55.6 5 5 3 3 0 dual b/y bias 62.769428 0.807456 506.2633 1011.526 -0.0071 -7.0 -0.7000000000000002 5.24 3623.1 nuORFdb_human ENST00000552593.1_1_12:53575584-53575683:+ ENST00000552593.1_1_12:53575584-53575683:+ ZNF740 ncRNA Retained Intron lncRNA GN=ZNF740 ncRNA Retained Intron E_20221201_NO30_400nL_HLAc1_1e7_directIP_titration_rep3_Slot2-3_1_3514 20mio-rep1 E_20221201_NO30_400nL_HLAc1_2e7_directIP_titration_rep1_Slot2-4_1_3515.18192.1577.2 2 2 1 10.16 7.58 2.59 2.59 88.7 5 0 0 6650.0 Hydrogen 24 (G) 9 HEGVATVIW (-) 9 PDPREVLW H E G V\A/T\V|I\W 55.6 5 4 2 1 0 b-ion bias 62.827599 0.764797 506.2651 1011.526 -0.0031 -3.0 3.2 5.24 3623.1 nuORFdb_human ENST00000552593.1_1_12:53575584-53575683:+ ENST00000552593.1_1_12:53575584-53575683:+ ZNF740 ncRNA Retained Intron lncRNA GN=ZNF740 ncRNA Retained Intron E_20221201_NO30_400nL_HLAc1_2e7_directIP_titration_rep1_Slot2-4_1_3515 20mio-rep2 E_20221201_NO30_400nL_HLAc1_2e7_directIP_titration_rep3_Slot2-4_1_3518.18262.1498.2 2 3 1 10.75 8.94 1.96 1.81 91.9 5 0 0 28400.0 Hydrogen 24 (G) 9 HEGVATVIW (-) 9 QLSEHVIW H E G V\A\T\V|I\W 55.6 5 5 1 1 0 b-ion bias 63.078686 0.814817 506.2631 1011.526 -0.0071 -7.0 -0.7000000000000002 5.24 3623.1 nuORFdb_human ENST00000552593.1_1_12:53575584-53575683:+ ENST00000552593.1_1_12:53575584-53575683:+ ZNF740 ncRNA Retained Intron lncRNA GN=ZNF740 ncRNA Retained Intron E_20221201_NO30_400nL_HLAc1_2e7_directIP_titration_rep3_Slot2-4_1_3518 20mio-rep3 E_20221201_NO30_400nL_HLAc1_2e7_directIP_titration_rep4_Slot2-4_1_3519.18240.1499.2 2 4 1 13.24 10.13 3.1 3.1 90.9 5 0 0 44200.0 Hydrogen 24 (G) 9 HEGVATVIW (-) 9 PDPREVLW H E G V|A\T|V|I\W 55.6 5 5 3 3 0 dual b/y bias 62.998123 0.814433 506.2633 1011.526 -0.0071 -7.0 -1.2000000000000002 5.24 3623.1 nuORFdb_human ENST00000552593.1_1_12:53575584-53575683:+ ENST00000552593.1_1_12:53575584-53575683:+ ZNF740 ncRNA Retained Intron lncRNA GN=ZNF740 ncRNA Retained Intron E_20221201_NO30_400nL_HLAc1_2e7_directIP_titration_rep4_Slot2-4_1_3519 40mio-rep1 E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep1_Slot1-04_1_3496.18405.1480.2 2 3 1 12.83 9.73 3.1 3.1 95.0 5 0 0 52700.0 Hydrogen 24 (G) 9 HEGVATVIW (-) 9 PDPREVLW H E G V\A\T\V|I\W 55.6 5 5 1 1 0 b-ion bias 63.57732 0.825945 506.2641 1011.526 -0.0051 -5.0 -1.7999999999999998 5.24 3623.1 nuORFdb_human ENST00000552593.1_1_12:53575584-53575683:+ ENST00000552593.1_1_12:53575584-53575683:+ ZNF740 ncRNA Retained Intron lncRNA GN=ZNF740 ncRNA Retained Intron E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep1_Slot1-04_1_3496 40mio-rep2 E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep2_Slot2-5_1_3520.18272.1494.2 2 5 1 12.18 9.08 3.09 3.09 90.4 6 0 0 36600.0 Hydrogen 24 (G) 9 HEGVATVIW (-) 9 PDPREVLW H E G\V\A\T|V|I\W 66.7 6 6 2 2 0 b-ion bias 63.121792 0.817394 506.2653 1011.526 -0.0031 -3.0 1.9000000000000004 5.24 3623.1 nuORFdb_human ENST00000552593.1_1_12:53575584-53575683:+ ENST00000552593.1_1_12:53575584-53575683:+ ZNF740 ncRNA Retained Intron lncRNA GN=ZNF740 ncRNA Retained Intron E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep2_Slot2-5_1_3520 40mio-rep3 E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep3_Slot2-5_1_3521.18074.1501.2 2 5 1 12.83 9.57 3.57 3.26 94.2 5 0 0 65600.0 Hydrogen 24 (G) 9 HEGVATVIW (-) 9 ASPTHISLW H E G V\A|T\V|I\W 55.6 5 5 2 2 0 b-ion bias 62.436443 0.813148 506.2632 1011.526 -0.0071 -7.0 -2.4000000000000004 5.24 3623.1 nuORFdb_human ENST00000552593.1_1_12:53575584-53575683:+ ENST00000552593.1_1_12:53575584-53575683:+ ZNF740 ncRNA Retained Intron lncRNA GN=ZNF740 ncRNA Retained Intron E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep3_Slot2-5_1_3521 40mio-rep4 E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep4_Slot2-5_1_3522.18097.1492.2 2 3 1 11.09 8.52 2.83 2.57 88.2 6 0 0 29700.0 Hydrogen 24 (G) 9 HEGVATVIW (-) 9 QLSEHVIW H E\G V\A\T\V|I\W 55.6 5 6 1 1 0 b-ion bias 62.498188 0.818565 506.2638 1011.526 -0.0051 -5.0 -0.2999999999999998 5.24 3623.1 nuORFdb_human ENST00000552593.1_1_12:53575584-53575683:+ ENST00000552593.1_1_12:53575584-53575683:+ ZNF740 ncRNA Retained Intron lncRNA GN=ZNF740 ncRNA Retained Intron E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep4_Slot2-5_1_3522 5mio-rep1 E_20221201_NO30_400nL_HLAc1_5e6_directIP_titration_rep1_Slot1-37_1_3502.18529.1523.2 2 1 1 9.67 8.14 1.02 1.53 88.7 5 0 0 4620.0 Hydrogen 24 (G) 9 HEGVATVIW (-) 9 PDPREVLW H E G\V\A T\V|I\W 44.4 4 5 1 1 0 b-ion bias 63.999607 0.798835 506.2628 1011.526 -0.0071 -7.0 0.0 5.24 3623.1 nuORFdb_human ENST00000552593.1_1_12:53575584-53575683:+ ENST00000552593.1_1_12:53575584-53575683:+ ZNF740 ncRNA Retained Intron lncRNA GN=ZNF740 ncRNA Retained Intron E_20221201_NO30_400nL_HLAc1_5e6_directIP_titration_rep1_Slot1-37_1_3502 40mio-rep1 E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep1_Slot1-04_1_3496.12915.1320.2 2 2 1 9.71 6.75 3.52 2.95 69.9 5 0 0 15900.0 M9m Hydrogen 4 (N) 9 HLDHLmTLY (R) 9 TmGAICTLLY H L D H\L\m\T|L\Y 55.6 5 5 1 1 0 b-ion bias m:Oxidized methionine 44.612499 0.926561 579.7827 1142.566 15.9924 -2.1 1.1 5.97 3302.8 nuORFdb_human ENST00000506451.1_1_4:120415631-120418847:+ ENST00000506451.1_1_4:120415631-120418847:+|ENST00000510011.5_1_4:120415631-120418987:+ RP11-33B1.1 Pseudogene Pseudogene GN=RP11-33B1.1 Pseudogene E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep1_Slot1-04_1_3496 Eva-50mio-3fr AL20210827_EV_HLA_PDAC_PANFRO413_2D_50e6_W632_classI_fxn2.36019.36019.2 2 2 2 11.02 8.54 3.35 2.48 72.2 6 0 0 13600000.0 Hydrogen 34 (N) 9 HPAGVQLIF (S) 9 FRGTGALIF H P\A G\V\Q\L|I/F 55.6 5 5 2 1 0 b-ion bias 0.994 100.0 65.98 100.0 0.0 491.2789 981.552 -0.0011 -1.1 -1.4000000000000001 6.74 6299.3 nuORFdb_human ENST00000376014.7_1_1:15537009-15541518:+ ENST00000376014.7_1_1:15537009-15541518:+ TMEM51 5' uORF 5' uORF GN=TMEM51 5' uORF AL20210827_EV_HLA_PDAC_PANFRO413_2D_50e6_W632_classI_fxn2 Exploris_FAIMS VH20221207_MKP_FAIMS2022E_HLAc1_4e7_PDAC.51321.51321.2 2 1 1 11.7 8.39 3.65 3.3 69.4 6 0 0 14400000.0 Hydrogen 34 (N) 9 HPAGVQLIF (S) 9 FRGTGALIF H P\A G\V\Q\L|I|F 55.6 5 6 2 2 0 b-ion bias -22.66 97.6 90.4 25.99 -50.0 491.2789 981.552 -0.001 -1.1 1.5 6.74 6299.3 nuORFdb_human ENST00000376014.7_1_1:15537009-15541518:+ ENST00000376014.7_1_1:15537009-15541518:+ TMEM51 5' uORF 5' uORF GN=TMEM51 5' uORF VH20221207_MKP_FAIMS2022E_HLAc1_4e7_PDAC SCP E_20221201_NO30_400nL_HLAc1_4e7_PDAC_Slot2-6_1_3524.4982.1459.2 2 1 1 11.1 7.36 4.81 3.74 78.6 6 0 0 3630.0 Hydrogen 19 (F) 9 HPHTTSLTY (-) 9 nVGKTCLTY H/P/H T T\S\L|T\Y 66.7 6 4 3 1 0 unbiased 17.219778 0.839443 528.7566 1056.511 -0.0042 -3.9 -1.5 6.92 3422.9 nuORFdb_human ENST00000339697.4_1_6:122774985-122775312:- ENST00000339697.4_1_6:122774985-122775312:- SERINC1 Out-of-Frame Out-of-Frame GN=SERINC1 Out-of-Frame E_20221201_NO30_400nL_HLAc1_4e7_PDAC_Slot2-6_1_3524 SCP E_20221201_NO30_400nL_HLAc1_4e7_PDAC_Slot2-6_1_3524.10749.1446.2 2 3 1 10.54 5.82 2.95 4.72 73.8 5 0 0 26900.0 Hydrogen 9 (M) 9 HPIVTCQSL (-) 9 DYMGLKGASL H P I V|T\C\Q\S\L 55.6 5 5 1 1 0 b-ion bias Carbamidomethylation C:Carbamidomethylation, 37.135597 0.847485 527.7697 1054.535 -0.0023 -2.2 0.19999999999999973 6.74 2020.4 nuORFdb_human ENST00000274030.10_1_4:120160700-120160751:+ ENST00000274030.10_1_4:120160700-120160751:+ USP53 5' uORF 5' uORF GN=USP53 5' uORF E_20221201_NO30_400nL_HLAc1_4e7_PDAC_Slot2-6_1_3524 SCP E_20221201_NO30_400nL_HLAc1_4e7_PDAC_Slot2-6_1_3524.12227.1265.2 2 1 1 10.37 6.54 4.58 3.83 73.9 7 0 0 10100.0 Hydrogen 13 (A) 11 HPLTCAHCLAL (P) 11 HFFnVSDELAL H/P|L/T C A H\C\L\A\L 63.6 7 5 3 1 0 unbiased Carbamidomethylation C:Carbamidomethylation, 42.240524 0.960791 646.8146 1292.624 -0.0011 -0.9 1.5 6.92 12588.4 smORF_v4|nuORFdb_human LINC00649_21:35303728-35318139:+ LINC00649_21:35303728-35318139:+|LOC440173_9:89656978-89625125:+|ENST00000580781.5_1_18:976625-1162827:+|ENST00000606016.1_1_7:8343401-8380111:+|T077605_12:47820665-47820896:-|T203106_2:174397838-174562964:+|T204263_2:186289930-186596461:+|T204261_2:186289981-186367408:+|T204262_2:186289981-186596987:+|T261295_4:10830697-10872649:+ LINC00649 lncRNA 21:35303728-35318139:+ 21 RP11-78F17.1 lincRNA lncRNA GN=LINC00649 E_20221201_NO30_400nL_HLAc1_4e7_PDAC_Slot2-6_1_3524 Eva-50mio-3fr AL20210827_EV_HLA_PDAC_PANFRO413_2D_50e6_W632_classI_fxn2.20801.20801.2 2 2 2 12.08 7.33 5.94 4.75 76.5 6 0 0 8470000.0 Hydrogen 99 (S) 9 HPLVSGHIL (S) 9 HPHLTAALI H/P|L/V S G\H|I-L 66.7 6 3 4 2 1 unbiased 1.0 68.1 39.45 82.47 0.0 486.7849 972.562 0.0001 0.1 -0.19999999999999998 6.92 14269.1 nuORFdb_human ENST00000414046.2_1_6:31431445-31431841:+ ENST00000414046.2_1_6:31431445-31431841:+ HCP5 Sense Overlapping Other GN=HCP5 Sense Overlapping AL20210827_EV_HLA_PDAC_PANFRO413_2D_50e6_W632_classI_fxn2 Exploris_FAIMS VH20221207_MKP_FAIMS2022E_HLAc1_4e7_PDAC.32023.32023.2 2 2 1 12.48 8.22 5.32 4.26 82.9 6 0 0 42600000.0 Hydrogen 99 (S) 9 HPLVSGHIL (S) 9 HPHLTAALI H/P\L-V S G\H|I-L 66.7 6 3 2 1 2 unbiased 1.0 88.0 61.83 21.6 -70.0 486.7836 972.562 -0.0025 -2.6 0.0 6.92 14269.1 nuORFdb_human ENST00000414046.2_1_6:31431445-31431841:+ ENST00000414046.2_1_6:31431445-31431841:+ HCP5 Sense Overlapping Other GN=HCP5 Sense Overlapping VH20221207_MKP_FAIMS2022E_HLAc1_4e7_PDAC Eva-50mio-3fr AL20210827_EV_HLA_PDAC_PANFRO413_2D_50e6_W632_classI_fxn2.21473.21473.2 2 1 1 9.57 6.49 2.31 3.08 67.1 7 0 0 2860000.0 Hydrogen 60 (H) 9 HPQPGVPAL (Y) 9 PAFRGSAPL H P\Q\P-G\V|P-A|L 77.8 7 5 2 2 2 b-ion bias 0.888 100.0 40.57 100.0 0.0 458.2561 915.505 0.0002 0.2 -0.09999999999999998 6.74 8174.1 nuORFdb_human ENST00000585374.5_1_19:5714280-5720143:- ENST00000585374.5_1_19:5714280-5720143:-|ENST00000360614.7_1_19:5714280-5720009:-|ENST00000590728.1_1_19:5715034-5720009:-|ENST00000590511.1_1_19:5717496-5719991:-|ENST00000593119.5_1_19:5714280-5720171:- LONP1 Out-of-Frame Out-of-Frame GN=LONP1 Out-of-Frame AL20210827_EV_HLA_PDAC_PANFRO413_2D_50e6_W632_classI_fxn2 Eva-50mio-3fr AL20210827_EV_HLA_PDAC_PANFRO413_2D_50e6_W632_classI_fxn3.15185.15185.2 2 2 2 12.82 6.93 5.39 5.89 88.6 7 0 1 22000000.0 Hydrogen 19 (G) 9 HPQPRPVAF (A) 9 HPQGPRTQK H/P|Q/P-R P-V/A|F 77.8 7 2 5 2 2 y-ion bias 0.959 95.5 29.13 100.0 0.0 524.7878 1048.569 -0.0004 -0.4 -0.9 9.75 11008.1 nuORFdb_human ENST00000301073.3_1_19:56111763-56113741:+ ENST00000301073.3_1_19:56111763-56113741:+|ENST00000591046.1_1_19:56113414-56113741:+ ZNF524 5' Overlap uORF 5' Overlap uORF GN=ZNF524 5' Overlap uORF AL20210827_EV_HLA_PDAC_PANFRO413_2D_50e6_W632_classI_fxn3 Exploris AL20221201_MKP_PDAC_01.18255.18380.2 2 2 1 10.45 6.5 4.02 3.95 71.3 7 0 1 221000000.0 Hydrogen 19 (G) 9 HPQPRPVAF (A) 9 HPQGPRTQK H/P|Q|P-R P-V/A|F 77.8 7 3 5 3 2 dual b/y bias 0.998 87.6 32.28 100.0 0.0 524.7885 1048.569 0.001 1.0 0.19999999999999996 9.75 11008.1 nuORFdb_human ENST00000301073.3_1_19:56111763-56113741:+ ENST00000301073.3_1_19:56111763-56113741:+|ENST00000591046.1_1_19:56113414-56113741:+ ZNF524 5' Overlap uORF 5' Overlap uORF GN=ZNF524 5' Overlap uORF AL20221201_MKP_PDAC_01 SCP E_20221201_NO30_400nL_HLAc1_4e7_PDAC_Slot2-6_1_3524.7901.1371.2 2 1 1 10.92 7.25 5.01 3.67 87.1 6 0 1 77400.0 Hydrogen 19 (G) 9 HPQPRPVAF (A) 9 HAGPFLFLF H/P/Q|P R\P V\A\F 55.6 5 4 3 1 0 unbiased 27.297445 0.894612 524.7879 1048.569 0.0 0.0 2.4 9.75 11008.1 nuORFdb_human ENST00000301073.3_1_19:56111763-56113741:+ ENST00000301073.3_1_19:56111763-56113741:+|ENST00000591046.1_1_19:56113414-56113741:+ ZNF524 5' Overlap uORF 5' Overlap uORF GN=ZNF524 5' Overlap uORF E_20221201_NO30_400nL_HLAc1_4e7_PDAC_Slot2-6_1_3524 SCP E_20221201_NO30_400nL_HLAc1_4e7_PDAC_Slot2-6_1_3524.3571.1569.3 3 1 1 8.02 5.5 2.51 2.52 73.3 6 2 2 7470.0 Hydrogen 39 (V) 10 HPRVAEGTRL (-) 10 LESGGIPGRIH H/P/R/V A/E/G T R-L 50.0 5 0 5 0 1 y-ion bias 12.349597 0.770037 379.2138 1135.633 -0.0056 -4.9 -2.5000000000000004 9.61 5242.0 nuORFdb_human ENST00000352480.9_1_9:133320329-133327701:+ ENST00000352480.9_1_9:133320329-133327701:+ ASS1 5' Overlap uORF 5' Overlap uORF GN=ASS1 5' Overlap uORF E_20221201_NO30_400nL_HLAc1_4e7_PDAC_Slot2-6_1_3524 Eva-50mio-3fr AL20210827_EV_HLA_PDAC_PANFRO413_2D_50e6_W632_classI_fxn3.24952.25052.2 2 2 2 16.02 10.74 11.35 5.28 91.2 7 1 1 38700000.0 Hydrogen 30 (R) 9 HPSLPVIKY (S) 9 LHPSIPVYK H/P\S L|P\V\I|K-Y 77.8 7 5 3 2 1 unbiased 0.982 85.5 46.48 100.0 0.0 527.3089 1053.609 0.0014 1.3 0.8 8.6 4481.1 nuORFdb_human ENST00000389561.6_1_12:132445225-132445345:+ ENST00000389561.6_1_12:132445225-132445345:+ EP400 Out-of-Frame Out-of-Frame GN=EP400 Out-of-Frame AL20210827_EV_HLA_PDAC_PANFRO413_2D_50e6_W632_classI_fxn3 SCP E_20221201_NO30_400nL_HLAc1_4e7_PDAC_Slot2-6_1_3524.14754.1404.2 2 2 1 15.87 7.5 9.83 8.37 86.8 6 1 1 51800.0 Hydrogen 30 (R) 9 HPSLPVIKY (S) 9 AMITTLLKY H/P|S L|P V|I|K\Y 55.6 5 5 5 4 0 dual b/y bias 50.972427 0.873942 527.3075 1053.609 -0.0024 -2.3 0.10000000000000009 8.6 4481.1 nuORFdb_human ENST00000389561.6_1_12:132445225-132445345:+ ENST00000389561.6_1_12:132445225-132445345:+ EP400 Out-of-Frame Out-of-Frame GN=EP400 Out-of-Frame E_20221201_NO30_400nL_HLAc1_4e7_PDAC_Slot2-6_1_3524 Exploris_FAIMS VH20221207_MKP_FAIMS2022E_HLAc1_4e7_PDAC.41071.41206.2 2 6 1 14.9 10.58 8.33 4.32 87.6 7 1 1 195000000.0 Hydrogen 30 (R) 9 HPSLPVIKY (S) 9 LHPSIPVYK H P\S-L|P-V\I|K/Y 77.8 7 4 3 2 2 unbiased 0.988 88.0 75.67 120.0 -70.0 527.3066 1053.609 -0.0031 -3.0 -0.3999999999999999 8.6 4481.1 nuORFdb_human ENST00000389561.6_1_12:132445225-132445345:+ ENST00000389561.6_1_12:132445225-132445345:+ EP400 Out-of-Frame Out-of-Frame GN=EP400 Out-of-Frame VH20221207_MKP_FAIMS2022E_HLAc1_4e7_PDAC SCP E_20221201_NO30_400nL_HLAc1_4e7_PDAC_Slot2-6_1_3524.10463.415.1 1 2 1 17.95 5.51 10.43 12.44 99.1 8 0 0 48500.0 Hydrogen 29 (M) 9 HPSSSVVIY (R) 9 ITDQVHLY H/P|S\S\S\V|V\I\Y 100.0 9 7 3 2 0 b-ion bias 36.148129 1.476566 988.5061 988.51 -0.0038 -3.9 -1.5 6.74 8761.4 nuORFdb_human ENST00000420613.6_1_12:49073557-49075333:- ENST00000420613.6_1_12:49073557-49075333:-|ENST00000548254.1_1_12:49075150-49075270:- KANSL2 Out-of-Frame Out-of-Frame GN=KANSL2 Out-of-Frame E_20221201_NO30_400nL_HLAc1_4e7_PDAC_Slot2-6_1_3524 Exploris AL20221201_MKP_PDAC_01.13158.13158.2 2 1 1 7.07 3.08 3.99 3.99 57.3 7 0 1 6100000.0 Hydrogen 17 (R) 9 HPVSQARPF (-) 9 HPVKKWPm H/P|V/S/Q/A R/P\F 77.8 7 2 6 1 0 y-ion bias 0.948 100.0 24.17 100.0 0.0 519.7783 1038.548 0.0013 1.3 0.5 9.75 2685.1 nuORFdb_human ENST00000588880.5_1_17:75137105-75137180:+ ENST00000588880.5_1_17:75137105-75137180:+ SEC14L1 5' uORF 5' uORF GN=SEC14L1 5' uORF AL20221201_MKP_PDAC_01 SCP E_20221201_NO30_400nL_HLAc1_4e7_PDAC_Slot2-6_1_3524.5498.1454.2 2 2 1 7.44 4.58 2.02 2.86 67.1 5 0 1 13800.0 Hydrogen 17 (R) 9 HPVSQARPF (-) 9 HPVACLKVn H/P|V|S/Q A R P-F 55.6 5 2 4 2 1 y-ion bias 19.001278 0.842567 519.7761 1038.548 -0.0032 -3.1 -0.7000000000000002 9.75 2685.1 nuORFdb_human ENST00000588880.5_1_17:75137105-75137180:+ ENST00000588880.5_1_17:75137105-75137180:+ SEC14L1 5' uORF 5' uORF GN=SEC14L1 5' uORF E_20221201_NO30_400nL_HLAc1_4e7_PDAC_Slot2-6_1_3524 Exploris_FAIMS VH20221207_MKP_FAIMS2022E_HLAc1_4e7_PDAC.17312.17312.2 2 1 1 8.01 6.39 2.25 1.62 84.4 5 0 1 1350000.0 Hydrogen 17 (R) 9 HPVSQARPF (-) 9 HPYIKFSF H/P|V/S Q A R/P\F 55.6 5 2 4 1 0 y-ion bias 0.909 80.2 37.15 120.0 -70.0 519.776 1038.548 -0.0031 -3.0 -0.3999999999999999 9.75 2685.1 nuORFdb_human ENST00000588880.5_1_17:75137105-75137180:+ ENST00000588880.5_1_17:75137105-75137180:+ SEC14L1 5' uORF 5' uORF GN=SEC14L1 5' uORF VH20221207_MKP_FAIMS2022E_HLAc1_4e7_PDAC Exploris_FAIMS VH20221207_MKP_FAIMS2022E_HLAc1_4e7_PDAC.35167.35167.2 2 1 1 8.82 6.78 1.47 2.04 70.5 5 0 0 9980000.0 Hydrogen 106 (R) 9 HPYPTSPPL (H) 9 HPTPQCRL H/P|Y/P T-S P P/L 44.4 4 1 4 1 1 y-ion bias 0.999 88.1 66.3 26.16 -70.0 504.7587 1008.515 -0.0048 -4.8 -2.1999999999999997 6.74 12858.4 nuORFdb_human ENST00000469505.1_1_X:47428924-47429504:+ ENST00000469505.1_1_X:47428924-47429504:+ ARAF ncRNA Processed Transcript lncRNA GN=ARAF ncRNA Processed Transcript VH20221207_MKP_FAIMS2022E_HLAc1_4e7_PDAC 40mio-rep2 E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep2_Slot2-5_1_3520.6207.1480.2 2 1 1 7.03 3.36 3.0 3.68 66.0 6 1 1 12400.0 Hydrogen 55 (F) 9 HSAPVVRSL (R) 9 HSAKLLAEP H/S/A|P/V V R\S-L 66.7 6 2 4 1 1 y-ion bias 21.449785 0.826023 483.2761 965.553 -0.008 -8.3 -3.4000000000000004 9.75 7719.9 nuORFdb_human ENST00000295924.11_2_3:156392382-156395461:+ ENST00000295924.11_2_3:156392382-156395461:+ TIPARP 5' uORF 5' uORF GN=TIPARP 5' uORF E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep2_Slot2-5_1_3520 40mio-rep1 E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep1_Slot1-04_1_3496.14448.1411.2 2 1 1 11.79 8.3 5.59 3.48 76.0 5 0 0 12000.0 Hydrogen 19 (S) 10 HSITQAGVQW (C) 10 KTSSGFSVQW H S I T Q\A|G|V|Q\W 50.0 5 5 3 3 0 unbiased 49.908024 0.869166 563.7847 1126.564 -0.0013 -1.1 2.1 6.74 5727.4 nuORFdb_human ENST00000216410.7_1_14:53242489-53242642:- ENST00000216410.7_1_14:53242489-53242642:-|ENST00000576713.6_1_17:79534551-79539120:- GNPNAT1 3' dORF 3' dORF GN=GNPNAT1 3' dORF E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep1_Slot1-04_1_3496 40mio-rep1 E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep1_Slot1-04_1_3496.23947.1323.2 2 1 1 10.06 3.78 3.83 6.28 77.7 6 0 0 6140.0 Hydrogen 57 (A) 10 HTLVTEPPFW (L) 10 VWYKMQSVSV H T\L V\T\E|P|P F\W 40.0 4 6 2 2 0 b-ion bias 82.726173 0.924336 613.8092 1226.62 -0.0097 -7.9 -4.7 5.24 8386.3 nuORFdb_human ENST00000528182.5_2_11:111957606-111959624:+ ENST00000528182.5_2_11:111957606-111959624:+|ENST00000414693.1_1_1:25620931-25621036:- SDHD 5' Overlap uORF 5' Overlap uORF GN=SDHD 5' Overlap uORF E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep1_Slot1-04_1_3496 Eva-50mio-3fr AL20210827_EV_HLA_PDAC_PANFRO413_2D_50e6_W632_classI_fxn1.16801.16801.2 2 1 1 10.2 4.85 5.35 5.35 73.8 6 0 0 156000000.0 Hydrogen 106 (P) 9 HVSEVTQAL (A) 9 TVSnIHQAI H V\S E\V\T\Q|A/L 55.6 5 5 2 1 0 b-ion bias 0.996 60.7 31.9 100.0 0.0 492.2612 983.516 -0.0005 -0.5 -0.6 5.24 12485.8 nuORFdb_human ENST00000528087.1_1_11:67120378-67120948:- ENST00000528087.1_1_11:67120378-67120948:- POLD4 5' Overlap uORF 5' Overlap uORF GN=POLD4 5' Overlap uORF AL20210827_EV_HLA_PDAC_PANFRO413_2D_50e6_W632_classI_fxn1 Exploris AL20221201_MKP_PDAC_01.21619.21619.2 2 1 1 7.22 3.16 3.84 4.06 61.2 6 0 0 1330000000.0 Hydrogen 106 (P) 9 HVSEVTQAL (A) 9 qLQQATQAL H V\S E\V\T\Q|A|L 55.6 5 6 2 2 0 b-ion bias 0.996 52.6 36.32 100.0 0.0 492.2622 983.516 0.0016 1.6 0.8 5.24 12485.8 nuORFdb_human ENST00000528087.1_1_11:67120378-67120948:- ENST00000528087.1_1_11:67120378-67120948:- POLD4 5' Overlap uORF 5' Overlap uORF GN=POLD4 5' Overlap uORF AL20221201_MKP_PDAC_01 Exploris_FAIMS VH20221207_MKP_FAIMS2022E_HLAc1_4e7_PDAC.26482.26482.2 2 2 1 10.62 4.91 5.72 5.72 64.4 7 0 0 655000000.0 Hydrogen 106 (P) 9 HVSEVTQAL (A) 9 TVSnIHQAI H V\S\E\V\T\Q|A/L 77.8 7 6 2 1 0 b-ion bias 0.99 42.8 52.78 23.47 -70.0 492.2603 983.516 -0.0023 -2.4 0.20000000000000018 5.24 12485.8 nuORFdb_human ENST00000528087.1_1_11:67120378-67120948:- ENST00000528087.1_1_11:67120378-67120948:- POLD4 5' Overlap uORF 5' Overlap uORF GN=POLD4 5' Overlap uORF VH20221207_MKP_FAIMS2022E_HLAc1_4e7_PDAC Eva-50mio-3fr AL20210827_EV_HLA_PDAC_PANFRO413_2D_50e6_W632_classI_fxn2.22173.22173.2 2 1 1 8.49 6.37 2.77 2.11 59.5 5 0 0 20100000.0 Hydrogen 16 (A) 9 HVVSPTSPF (-) 9 VSTHVPSFP H V\V S\P T|S|P-F 33.3 3 4 2 2 1 b-ion bias 0.999 90.4 41.83 100.0 0.0 485.753 970.499 -0.0006 -0.6 -0.8999999999999999 6.74 2714.2 nuORFdb_human ENST00000418819.5_3_X:102933501-102939656:- ENST00000418819.5_3_X:102933501-102939656:- MORF4L2 5' uORF 5' uORF GN=MORF4L2 5' uORF AL20210827_EV_HLA_PDAC_PANFRO413_2D_50e6_W632_classI_fxn2 Exploris AL20221201_MKP_PDAC_01.29233.29233.2 2 2 1 9.09 5.21 3.76 3.88 64.7 5 0 0 637000000.0 Hydrogen 16 (A) 9 HVVSPTSPF (-) 9 VSTHVPSFP H V\V S\P T/S|P/F 33.3 3 3 3 1 0 unbiased 0.856 70.3 47.28 18.05 0.0 485.7543 970.499 0.002 2.1 1.3 6.74 2714.2 nuORFdb_human ENST00000418819.5_3_X:102933501-102939656:- ENST00000418819.5_3_X:102933501-102939656:- MORF4L2 5' uORF 5' uORF GN=MORF4L2 5' uORF AL20221201_MKP_PDAC_01 SCP E_20221201_NO30_400nL_HLAc1_4e7_PDAC_Slot2-6_1_3524.7296.1558.3 3 4 1 17.78 5.78 10.64 12.0 93.3 8 0 1 82500.0 Hydrogen 108 (M) 10 HWNDTRPAHF (R) 10 HSEAQKYRDF H/W|N/D|T R/P\A\H\F 80.0 8 5 5 2 0 unbiased 25.209251 0.776877 427.5337 1280.592 -0.0045 -3.5 -1.1 6.92 16697.8 nuORFdb_human ENST00000521951.1_1_8:128806810-128903111:+ ENST00000521951.1_1_8:128806810-128903111:+ PVT1 lincRNA lncRNA GN=PVT1 lincRNA E_20221201_NO30_400nL_HLAc1_4e7_PDAC_Slot2-6_1_3524 Eva-50mio-3fr AL20210827_EV_HLA_PDAC_PANFRO413_2D_50e6_W632_classI_fxn3.30964.30964.2 2 1 1 13.06 2.36 7.56 10.7 81.9 8 1 1 19800000.0 Hydrogen 32 (N) 9 HYIIRVVSF (T) 9 WNLGIKmKK H/Y|I|I/R\V\V|S/F 100.0 9 5 6 3 0 dual b/y bias 0.998 97.2 56.88 34.96 0.0 567.327 1133.647 0.0002 0.2 -0.3 8.75 10013.6 nuORFdb_human ENST00000409714.7_1_2:170460711-170470975:+ ENST00000409714.7_1_2:170460711-170470975:+ PPIG Out-of-Frame Out-of-Frame GN=PPIG Out-of-Frame AL20210827_EV_HLA_PDAC_PANFRO413_2D_50e6_W632_classI_fxn3 Exploris_FAIMS VH20221207_MKP_FAIMS2022E_HLAc1_4e7_PDAC.62363.62363.2 2 1 1 13.33 4.38 6.87 8.95 81.2 8 1 1 283000000.0 Hydrogen 32 (N) 9 HYIIRVVSF (T) 9 HYAEIIIKF H/Y|I|I/R\V\V/S/F 100.0 9 4 6 2 0 unbiased 0.996 92.3 106.75 28.99 -70.0 567.3248 1133.647 -0.0042 -3.7 -1.1 8.75 10013.6 nuORFdb_human ENST00000409714.7_1_2:170460711-170470975:+ ENST00000409714.7_1_2:170460711-170470975:+ PPIG Out-of-Frame Out-of-Frame GN=PPIG Out-of-Frame VH20221207_MKP_FAIMS2022E_HLAc1_4e7_PDAC Exploris_FAIMS VH20221207_MKP_FAIMS2022E_HLAc1_4e7_PDAC.58378.58378.3 3 1 1 11.5 3.13 8.11 8.37 73.5 6 1 1 26100000.0 Hydrogen 4 (T) 9 HYINYKVHF (-) 9 KNERNYHIF H Y\I\N|Y|K V/H/F 55.6 5 4 4 2 0 unbiased 0.957 100.0 101.63 120.0 -50.0 407.544 1220.621 -0.0036 -3.0 -0.3999999999999999 8.51 1638.9 nuORFdb_human ENST00000357997.9_1_12:133331595-133331631:- ENST00000357997.9_1_12:133331595-133331631:- ANKLE2 Out-of-Frame Out-of-Frame GN=ANKLE2 Out-of-Frame VH20221207_MKP_FAIMS2022E_HLAc1_4e7_PDAC Exploris AL20221201_MKP_PDAC_01.62874.62874.2 2 2 1 10.76 1.62 7.73 9.14 74.6 8 1 1 14300000.0 Hydrogen 7 (M) 10 HYPELKLPYF (-) 10 TSTIKPYHRGF H Y|P\E-L-K-L/P/Y/F 80.0 8 2 4 1 3 y-ion bias 0.859 58.4 94.9 100.0 0.0 653.8433 1306.683 -0.0037 -2.8 -3.5999999999999996 6.75 2035.4 nuORFdb_human ENST00000290541.6_1_1:151374297-151374348:+ ENST00000290541.6_1_1:151374297-151374348:+ PSMB4 3' dORF 3' dORF GN=PSMB4 3' dORF AL20221201_MKP_PDAC_01 Exploris AL20221201_MKP_PDAC_01.14412.14538.2 2 1 1 16.38 7.71 7.95 8.67 86.6 6 1 1 216000000.0 Hydrogen 7 (V) 9 HYSKCTGVF (F) 9 NCGTWSKVF H/Y|S\K\C T G|V|F 66.7 6 5 4 3 0 dual b/y bias Carbamidomethylation C:Carbamidomethylation, 1.0 100.0 26.32 100.0 0.0 549.7556 1098.504 0.0003 0.3 -0.5 8.6 3004.6 smORF_v4|nuORFdb_human HNRNPC_14:21731784-21731858:- HNRNPC_14:21731784-21731858:-|ENST00000430246.6_1_14:21731784-21731862:- HNRNPC uORF 14:21731784-21731858:- 14 HNRNPC 5' uORF 5' uORF GN=HNRNPC AL20221201_MKP_PDAC_01 SCP E_20221201_NO30_400nL_HLAc1_4e7_PDAC_Slot2-6_1_3524.5801.1359.2 2 2 1 17.26 7.14 9.52 10.12 90.8 8 1 1 122000.0 Hydrogen 7 (V) 9 HYSKCTGVF (F) 9 RLGLDcGVF H/Y|S|K\C\T\G\V\F 100.0 9 7 3 2 0 b-ion bias Carbamidomethylation C:Carbamidomethylation, 20.047821 0.901925 549.7539 1098.504 -0.003 -2.7 -0.30000000000000027 8.6 3004.6 smORF_v4|nuORFdb_human HNRNPC_14:21731784-21731858:- HNRNPC_14:21731784-21731858:-|ENST00000430246.6_1_14:21731784-21731862:- HNRNPC uORF 14:21731784-21731858:- 14 HNRNPC 5' uORF 5' uORF GN=HNRNPC E_20221201_NO30_400nL_HLAc1_4e7_PDAC_Slot2-6_1_3524 Exploris_FAIMS VH20221207_MKP_FAIMS2022E_HLAc1_4e7_PDAC.19094.19218.2 2 1 1 11.57 4.37 3.86 7.2 72.9 6 1 1 75900000.0 Hydrogen 7 (V) 9 HYSKCTGVF (F) 9 AYHSKVSCF H Y|S\K\C T-G\V/F 55.6 5 4 2 1 1 b-ion bias Carbamidomethylation C:Carbamidomethylation, 0.996 100.0 40.63 9.71 -50.0 549.7537 1098.504 -0.0037 -3.3 -0.6999999999999997 8.6 3004.6 smORF_v4|nuORFdb_human HNRNPC_14:21731784-21731858:- HNRNPC_14:21731784-21731858:-|ENST00000430246.6_1_14:21731784-21731862:- HNRNPC uORF 14:21731784-21731858:- 14 HNRNPC 5' uORF 5' uORF GN=HNRNPC VH20221207_MKP_FAIMS2022E_HLAc1_4e7_PDAC Exploris AL20221201_MKP_PDAC_01.28423.28585.2 2 1 1 9.64 4.54 5.55 5.09 61.8 5 1 1 55800000.0 Hydrogen 7 (V) 10 HYSKCTGVFF (L) 10 TQSKGMWNFF H Y\S\K C T G\V|F/F 40.0 4 4 2 1 0 b-ion bias Carbamidomethylation C:Carbamidomethylation, 0.964 100.0 46.18 74.07 0.0 623.2909 1245.572 0.0024 1.9 1.0999999999999999 8.6 3004.6 smORF_v4|nuORFdb_human HNRNPC_14:21731784-21731858:- HNRNPC_14:21731784-21731858:-|ENST00000430246.6_1_14:21731784-21731862:- HNRNPC uORF 14:21731784-21731858:- 14 HNRNPC 5' uORF 5' uORF GN=HNRNPC AL20221201_MKP_PDAC_01 SCP E_20221201_NO30_400nL_HLAc1_4e7_PDAC_Slot2-6_1_3524.12004.1277.2 2 2 1 15.9 8.95 6.62 6.95 89.2 9 1 1 76300.0 Hydrogen 7 (V) 10 HYSKCTGVFF (L) 10 WNEGWIFFF H/Y|S\K\C\T\G\V|F\F 100.0 10 8 3 2 0 b-ion bias Carbamidomethylation C:Carbamidomethylation, 41.470009 0.952951 623.2879 1245.572 -0.0034 -2.7 -0.30000000000000027 8.6 3004.6 smORF_v4|nuORFdb_human HNRNPC_14:21731784-21731858:- HNRNPC_14:21731784-21731858:-|ENST00000430246.6_1_14:21731784-21731862:- HNRNPC uORF 14:21731784-21731858:- 14 HNRNPC 5' uORF 5' uORF GN=HNRNPC E_20221201_NO30_400nL_HLAc1_4e7_PDAC_Slot2-6_1_3524 Exploris_FAIMS VH20221207_MKP_FAIMS2022E_HLAc1_4e7_PDAC.34510.34510.2 2 1 1 11.9 5.93 5.28 5.97 74.2 5 1 1 19300000.0 Hydrogen 7 (V) 10 HYSKCTGVFF (L) 10 IQQmKMDFF H Y\S\K C T G\V|F/F 40.0 4 4 2 1 0 b-ion bias Carbamidomethylation C:Carbamidomethylation, 0.952 77.7 65.4 120.0 -50.0 623.2906 1245.572 0.0018 1.5 4.1 8.6 3004.6 smORF_v4|nuORFdb_human HNRNPC_14:21731784-21731858:- HNRNPC_14:21731784-21731858:-|ENST00000430246.6_1_14:21731784-21731862:- HNRNPC uORF 14:21731784-21731858:- 14 HNRNPC 5' uORF 5' uORF GN=HNRNPC VH20221207_MKP_FAIMS2022E_HLAc1_4e7_PDAC 40mio-rep3 E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep3_Slot2-5_1_3521.17400.635.1 1 1 1 8.7 6.37 1.79 2.32 76.0 5 0 0 3620.0 Hydrogen 7 (G) 9 IAEAGVSVV (L) 9 ADLGALSVV I A/E A G|V\S\V\V 44.4 4 4 2 1 0 b-ion bias 60.106312 1.345117 844.4731 844.477 -0.0045 -5.3 -0.7000000000000002 4.0 9415.7 nuORFdb_human ENST00000377748.5_1_1:6579769-6580027:- ENST00000377748.5_1_1:6579769-6580027:- PLEKHG5 5' uORF 5' uORF GN=PLEKHG5 5' uORF E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep3_Slot2-5_1_3521 20mio-rep1 E_20221201_NO30_400nL_HLAc1_2e7_directIP_titration_rep1_Slot2-4_1_3515.24405.620.1 1 2 1 9.7 3.63 6.38 6.08 82.5 7 0 0 5460.0 Hydrogen 29 (W) 9 IAGGPWAGW (S) 9 APWQGLQD I A/G/G/P/W\A\G\W 77.8 7 3 4 0 0 unbiased 84.275986 1.354366 914.4457 914.452 -0.0059 -6.5 -0.2999999999999998 5.52 7940.1 nuORFdb_human ENST00000369774.8_3_10:105376987-105386893:- ENST00000369774.8_3_10:105376987-105386893:- SH3PXD2A Out-of-Frame Out-of-Frame GN=SH3PXD2A Out-of-Frame E_20221201_NO30_400nL_HLAc1_2e7_directIP_titration_rep1_Slot2-4_1_3515 20mio-rep2 E_20221201_NO30_400nL_HLAc1_2e7_directIP_titration_rep3_Slot2-4_1_3518.24410.614.1 1 2 1 8.1 3.11 5.89 4.99 76.4 6 0 0 3990.0 Hydrogen 29 (W) 9 IAGGPWAGW (S) 9 APWQGLQD I A/G/G/P W\A\G\W 55.6 5 3 3 0 0 unbiased 84.312393 1.357963 914.4503 914.452 -0.0019 -2.1 4.199999999999999 5.52 7940.1 nuORFdb_human ENST00000369774.8_3_10:105376987-105386893:- ENST00000369774.8_3_10:105376987-105386893:- SH3PXD2A Out-of-Frame Out-of-Frame GN=SH3PXD2A Out-of-Frame E_20221201_NO30_400nL_HLAc1_2e7_directIP_titration_rep3_Slot2-4_1_3518 20mio-rep3 E_20221201_NO30_400nL_HLAc1_2e7_directIP_titration_rep4_Slot2-4_1_3519.24465.618.1 1 1 1 7.98 2.35 6.1 5.63 74.4 6 0 0 1980.0 Hydrogen 29 (W) 9 IAGGPWAGW (S) 9 APWQGLQD I A/G/G/P W\A\G\W 55.6 5 3 3 0 0 unbiased 84.49366 1.355593 914.4483 914.452 -0.0039 -4.3 1.5 5.52 7940.1 nuORFdb_human ENST00000369774.8_3_10:105376987-105386893:- ENST00000369774.8_3_10:105376987-105386893:- SH3PXD2A Out-of-Frame Out-of-Frame GN=SH3PXD2A Out-of-Frame E_20221201_NO30_400nL_HLAc1_2e7_directIP_titration_rep4_Slot2-4_1_3519 40mio-rep1 E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep1_Slot1-04_1_3496.24623.593.1 1 3 1 11.12 3.96 8.02 7.16 84.9 7 0 0 13000.0 Hydrogen 29 (W) 9 IAGGPWAGW (S) 9 APWQGLQD I/A/G/G/P W\A\G\W 77.8 7 3 4 0 0 unbiased 85.060295 1.370237 914.4485 914.452 -0.0029 -3.2 0.0 5.52 7940.1 nuORFdb_human ENST00000369774.8_3_10:105376987-105386893:- ENST00000369774.8_3_10:105376987-105386893:- SH3PXD2A Out-of-Frame Out-of-Frame GN=SH3PXD2A Out-of-Frame E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep1_Slot1-04_1_3496 40mio-rep2 E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep2_Slot2-5_1_3520.24392.609.1 1 2 1 11.2 3.5 7.5 7.71 83.7 7 0 0 7880.0 Hydrogen 29 (W) 9 IAGGPWAGW (S) 9 APWQGLQD I A/G/G|P\W\A\G\W 77.8 7 5 3 1 0 unbiased 84.261955 1.360731 914.45 914.452 -0.0019 -2.1 2.8000000000000003 5.52 7940.1 nuORFdb_human ENST00000369774.8_3_10:105376987-105386893:- ENST00000369774.8_3_10:105376987-105386893:- SH3PXD2A Out-of-Frame Out-of-Frame GN=SH3PXD2A Out-of-Frame E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep2_Slot2-5_1_3520 40mio-rep3 E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep3_Slot2-5_1_3521.24404.610.1 1 2 1 8.71 2.5 6.07 6.21 74.7 7 0 0 6240.0 Hydrogen 29 (W) 9 IAGGPWAGW (S) 9 HLAmLAGEG I/A/G/G/P W\A\G\W 77.8 7 3 4 0 0 unbiased 84.301384 1.360087 914.4465 914.452 -0.0059 -6.5 -1.9000000000000004 5.52 7940.1 nuORFdb_human ENST00000369774.8_3_10:105376987-105386893:- ENST00000369774.8_3_10:105376987-105386893:- SH3PXD2A Out-of-Frame Out-of-Frame GN=SH3PXD2A Out-of-Frame E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep3_Slot2-5_1_3521 40mio-rep4 E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep4_Slot2-5_1_3522.24404.605.1 1 2 1 9.59 3.24 6.77 6.34 75.4 7 0 0 6260.0 Hydrogen 29 (W) 9 IAGGPWAGW (S) 9 APWQGLQD I/A/G/G/P W\A\G\W 77.8 7 3 4 0 0 unbiased 84.289589 1.363548 914.4476 914.452 -0.0039 -4.3 0.40000000000000036 5.52 7940.1 nuORFdb_human ENST00000369774.8_3_10:105376987-105386893:- ENST00000369774.8_3_10:105376987-105386893:- SH3PXD2A Out-of-Frame Out-of-Frame GN=SH3PXD2A Out-of-Frame E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep4_Slot2-5_1_3522 SCP E_20221201_NO30_400nL_HLAc1_4e7_PDAC_Slot2-6_1_3524.19540.526.1 1 3 1 9.48 5.47 3.55 4.01 67.7 6 1 1 91600.0 M19m Hydrogen 13 (L) 8 IALKADmL (Y) 8 AmDLLKAI I/A/L/K|A|D m\L 75.0 6 3 5 2 0 unbiased m:Oxidized methionine 67.503357 1.410775 890.4971 874.507 15.9903 -5.1 -2.6999999999999997 5.84 4605.4 nuORFdb_human ENST00000537473.2_1_12:57058103-57058217:- ENST00000537473.2_1_12:57058103-57058217:- PTGES3 3' dORF 3' dORF GN=PTGES3 3' dORF E_20221201_NO30_400nL_HLAc1_4e7_PDAC_Slot2-6_1_3524 40mio-rep1 E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep1_Slot1-04_1_3496.21166.1293.2 2 1 1 7.85 7.85 7.85 55.5 9 1 1 35200.0 Hydrogen 25 (S) 10 IAWQSIQRSW (L) 10 I/A/W|Q/S/I/Q/R\S\W 100.0 10 3 7 1 0 y-ion bias 73.118004 0.943034 637.8317 1274.664 -0.0073 -5.7 -2.5 9.75 6111.1 nuORFdb_human ENST00000297579.9_1_8:104432557-104433221:+ ENST00000297579.9_1_8:104432557-104433221:+ DCAF13 Out-of-Frame Out-of-Frame GN=DCAF13 Out-of-Frame E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep1_Slot1-04_1_3496 Exploris_FAIMS VH20221207_MKP_FAIMS2022E_HLAc1_4e7_PDAC.62230.62545.2 2 1 1 9.75 7.22 -1.6 2.52 82.1 6 2 3 2060000000.0 Hydrogen 37 (K) 9 IDKYREVLK (A) 9 DRLKYLnLK I/D\K\Y R|E V|L/K 55.6 5 4 4 2 0 unbiased 0.994 99.0 107.1 0.63 -50.0 582.3432 1163.678 0.0009 0.8 3.4000000000000004 8.5 6001.9 nuORFdb_human ENST00000480739.2_1_12:58068877-58069030:+ ENST00000480739.2_1_12:58068877-58069030:+ RPL13AP23 Pseudogene Pseudogene GN=RPL13AP23 Pseudogene VH20221207_MKP_FAIMS2022E_HLAc1_4e7_PDAC 1mio-rep1 E_20221201_NO30_400nL_HLAc1_1e6_directIP_titration_rep1_Slot1-06_1_3498.13295.1486.2 2 1 1 8.72 6.63 3.12 2.09 74.4 6 1 1 3500.0 Hydrogen 8 (P) 9 IEVDGGRDW (S) 9 LEVnLSmSH I/E/V|D|G/G R D\W 66.7 6 3 5 2 0 unbiased 45.922474 0.822149 523.7471 1046.49 -0.0034 -3.3 1.6000000000000005 4.03 2399.6 nuORFdb_human ENST00000374677.7_1_6:33168901-33168970:+ ENST00000374677.7_1_6:33168901-33168970:+ SLC39A7 5' uORF 5' uORF GN=SLC39A7 5' uORF E_20221201_NO30_400nL_HLAc1_1e6_directIP_titration_rep1_Slot1-06_1_3498 10mio-rep1 E_20221201_NO30_400nL_HLAc1_1e7_directIP_titration_rep1_Slot2-3_1_3512.12927.1482.2 2 2 1 12.11 9.5 2.61 2.61 86.1 7 1 1 23600.0 Hydrogen 8 (P) 9 IEVDGGRDW (S) 9 LEVnLSmSH I/E|V|D|G/G R/D-W 77.8 7 3 6 3 1 dual b/y bias 44.661022 0.825089 523.745 1046.49 -0.0074 -7.1 0.6000000000000005 4.03 2399.6 nuORFdb_human ENST00000374677.7_1_6:33168901-33168970:+ ENST00000374677.7_1_6:33168901-33168970:+ SLC39A7 5' uORF 5' uORF GN=SLC39A7 5' uORF E_20221201_NO30_400nL_HLAc1_1e7_directIP_titration_rep1_Slot2-3_1_3512 10mio-rep2 E_20221201_NO30_400nL_HLAc1_1e7_directIP_titration_rep2_Slot2-3_1_3513.12883.1484.2 2 1 1 10.95 7.87 4.17 3.08 81.5 7 1 1 13400.0 Hydrogen 8 (P) 9 IEVDGGRDW (S) 9 LEVnLSmSH I/E/V|D|G/G/R\D W 77.8 7 3 6 2 0 y-ion bias 44.507542 0.823435 523.7448 1046.49 -0.0074 -7.1 -0.39999999999999947 4.03 2399.6 nuORFdb_human ENST00000374677.7_1_6:33168901-33168970:+ ENST00000374677.7_1_6:33168901-33168970:+ SLC39A7 5' uORF 5' uORF GN=SLC39A7 5' uORF E_20221201_NO30_400nL_HLAc1_1e7_directIP_titration_rep2_Slot2-3_1_3513 10mio-rep3 E_20221201_NO30_400nL_HLAc1_1e7_directIP_titration_rep3_Slot2-3_1_3514.12844.1487.2 2 1 1 13.76 11.18 2.64 2.57 91.3 7 1 1 10100.0 Hydrogen 8 (P) 9 IEVDGGRDW (S) 9 LEVnLSmSH I/E|V|D|G/G R|D-W 77.8 7 4 6 4 1 dual b/y bias 44.375401 0.821897 523.7462 1046.49 -0.0054 -5.2 1.0999999999999996 4.03 2399.6 nuORFdb_human ENST00000374677.7_1_6:33168901-33168970:+ ENST00000374677.7_1_6:33168901-33168970:+ SLC39A7 5' uORF 5' uORF GN=SLC39A7 5' uORF E_20221201_NO30_400nL_HLAc1_1e7_directIP_titration_rep3_Slot2-3_1_3514 20mio-rep1 E_20221201_NO30_400nL_HLAc1_2e7_directIP_titration_rep1_Slot2-4_1_3515.12828.1486.2 2 2 1 15.85 11.24 5.13 4.61 94.9 8 1 1 77900.0 Hydrogen 8 (P) 9 IEVDGGRDW (S) 9 LEVnLSmSH I/E|V|D|G/G/R\D-W 100.0 9 4 6 3 1 dual b/y bias 44.307705 0.822232 523.7455 1046.49 -0.0054 -5.2 1.0 4.03 2399.6 nuORFdb_human ENST00000374677.7_1_6:33168901-33168970:+ ENST00000374677.7_1_6:33168901-33168970:+ SLC39A7 5' uORF 5' uORF GN=SLC39A7 5' uORF E_20221201_NO30_400nL_HLAc1_2e7_directIP_titration_rep1_Slot2-4_1_3515 20mio-rep3 E_20221201_NO30_400nL_HLAc1_2e7_directIP_titration_rep4_Slot2-4_1_3519.12835.1483.2 2 2 1 12.47 9.61 2.9 2.85 91.0 7 1 1 59700.0 Hydrogen 8 (P) 9 IEVDGGRDW (S) 9 LEVnLSmSH I/E|V|D|G/G R/D-W 77.8 7 3 6 3 1 dual b/y bias 44.336361 0.823913 523.7468 1046.49 -0.0034 -3.3 2.5 4.03 2399.6 nuORFdb_human ENST00000374677.7_1_6:33168901-33168970:+ ENST00000374677.7_1_6:33168901-33168970:+ SLC39A7 5' uORF 5' uORF GN=SLC39A7 5' uORF E_20221201_NO30_400nL_HLAc1_2e7_directIP_titration_rep4_Slot2-4_1_3519 40mio-rep1 E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep1_Slot1-04_1_3496.13181.1475.2 2 3 1 17.39 12.79 4.62 4.6 96.1 8 1 1 142000.0 Hydrogen 8 (P) 9 IEVDGGRDW (S) 9 LEVnLSmSH I/E|V|D|G/G/R|D\W 100.0 9 5 7 4 0 dual b/y bias 45.531723 0.829121 523.747 1046.49 -0.0034 -3.3 -0.09999999999999964 4.03 2399.6 nuORFdb_human ENST00000374677.7_1_6:33168901-33168970:+ ENST00000374677.7_1_6:33168901-33168970:+ SLC39A7 5' uORF 5' uORF GN=SLC39A7 5' uORF E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep1_Slot1-04_1_3496 40mio-rep2 E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep2_Slot2-5_1_3520.12828.1487.2 2 2 1 15.97 10.23 4.23 5.74 95.5 7 1 1 160000.0 Hydrogen 8 (P) 9 IEVDGGRDW (S) 9 LEVnLSmSH I/E|V|D|G/G R\D\W 77.8 7 5 5 3 0 dual b/y bias 44.314404 0.821828 523.7473 1046.49 -0.0034 -3.3 1.6000000000000005 4.03 2399.6 nuORFdb_human ENST00000374677.7_1_6:33168901-33168970:+ ENST00000374677.7_1_6:33168901-33168970:+ SLC39A7 5' uORF 5' uORF GN=SLC39A7 5' uORF E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep2_Slot2-5_1_3520 40mio-rep3 E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep3_Slot2-5_1_3521.12828.1489.2 2 2 1 18.5 12.25 6.27 6.26 96.8 8 1 1 130000.0 Hydrogen 8 (P) 9 IEVDGGRDW (S) 9 LEVnLSmSH I/E|V|D|G/G/R\D\W 100.0 9 5 6 3 0 dual b/y bias 44.31879 0.820273 523.7464 1046.49 -0.0054 -5.2 -0.6000000000000005 4.03 2399.6 nuORFdb_human ENST00000374677.7_1_6:33168901-33168970:+ ENST00000374677.7_1_6:33168901-33168970:+ SLC39A7 5' uORF 5' uORF GN=SLC39A7 5' uORF E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep3_Slot2-5_1_3521 40mio-rep4 E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep4_Slot2-5_1_3522.12817.1489.2 2 2 1 15.98 10.27 4.2 5.71 95.6 7 1 1 121000.0 Hydrogen 8 (P) 9 IEVDGGRDW (S) 9 LEVnLSmSH I/E|V|D|G/G R\D\W 77.8 7 5 5 3 0 dual b/y bias 44.261238 0.820437 523.7467 1046.49 -0.0034 -3.3 1.4000000000000004 4.03 2399.6 nuORFdb_human ENST00000374677.7_1_6:33168901-33168970:+ ENST00000374677.7_1_6:33168901-33168970:+ SLC39A7 5' uORF 5' uORF GN=SLC39A7 5' uORF E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep4_Slot2-5_1_3522 5mio-rep1 E_20221201_NO30_400nL_HLAc1_5e6_directIP_titration_rep1_Slot1-37_1_3502.13183.1471.2 2 1 1 10.76 8.43 1.32 2.33 85.0 6 1 1 11600.0 Hydrogen 8 (P) 9 IEVDGGRDW (S) 9 LEVnLSmSH I/E/V|D|G/G R D-W 66.7 6 2 5 2 1 y-ion bias 45.537922 0.831592 523.7458 1046.49 -0.0054 -5.2 1.7999999999999998 4.03 2399.6 nuORFdb_human ENST00000374677.7_1_6:33168901-33168970:+ ENST00000374677.7_1_6:33168901-33168970:+ SLC39A7 5' uORF 5' uORF GN=SLC39A7 5' uORF E_20221201_NO30_400nL_HLAc1_5e6_directIP_titration_rep1_Slot1-37_1_3502 5mio-rep2 E_20221201_NO30_400nL_HLAc1_5e6_directIP_titration_rep2_Slot2-2_1_3510.13107.1485.2 2 1 1 11.09 9.05 1.48 2.04 85.6 7 1 1 7980.0 Hydrogen 8 (P) 9 IEVDGGRDW (S) 9 LEVnLSmSH I/E|V|D/G/G/R-D W 77.8 7 2 6 2 1 y-ion bias 45.270184 0.822971 523.7458 1046.49 -0.0054 -5.2 3.499999999999999 4.03 2399.6 nuORFdb_human ENST00000374677.7_1_6:33168901-33168970:+ ENST00000374677.7_1_6:33168901-33168970:+ SLC39A7 5' uORF 5' uORF GN=SLC39A7 5' uORF E_20221201_NO30_400nL_HLAc1_5e6_directIP_titration_rep2_Slot2-2_1_3510 5mio-rep3 E_20221201_NO30_400nL_HLAc1_5e6_directIP_titration_rep3_Slot2-2_1_3511.13116.1482.2 2 1 1 11.62 7.5 4.12 4.12 87.3 8 1 1 6810.0 Hydrogen 8 (P) 9 IEVDGGRDW (S) 9 LEVnLSmSH I/E|V|D/G/G/R\D\W 100.0 9 4 6 2 0 unbiased 45.308884 0.824753 523.7452 1046.49 -0.0074 -7.1 1.0999999999999996 4.03 2399.6 nuORFdb_human ENST00000374677.7_1_6:33168901-33168970:+ ENST00000374677.7_1_6:33168901-33168970:+ SLC39A7 5' uORF 5' uORF GN=SLC39A7 5' uORF E_20221201_NO30_400nL_HLAc1_5e6_directIP_titration_rep3_Slot2-2_1_3511 Exploris AL20221201_MKP_PDAC_01.65148.65148.2 2 1 1 13.12 8.53 5.17 4.59 86.7 7 1 1 92200000.0 Hydrogen 2 (M) 9 IFDFTCMRF (-) 9 FLDFMTCFR I/F|D|F/T C/M/R/F 77.8 7 2 7 2 0 y-ion bias Carbamidomethylation C:Carbamidomethylation, 0.937 92.2 98.27 69.16 0.0 618.7812 1236.554 0.0011 0.9 0.09999999999999998 5.84 1367.6 nuORFdb_human ENST00000400227.7_1_20:478366-478399:- ENST00000400227.7_1_20:478366-478399:- CSNK2A1 Out-of-Frame Out-of-Frame GN=CSNK2A1 Out-of-Frame AL20221201_MKP_PDAC_01 Exploris AL20221201_MKP_PDAC_01.66067.66067.2 2 1 1 11.64 6.89 4.14 4.75 76.9 7 0 0 285000000.0 Hydrogen 21 (F) 10 IFIADTCLSF (-) 10 SLFLDNPAYF I F\I\A D-T\C\L/S/F 60.0 6 4 2 0 1 b-ion bias Carbamidomethylation C:Carbamidomethylation, 0.689 84.7 98.73 4.76 0.0 593.7944 1186.581 0.0002 0.2 -0.6000000000000001 3.8 3488.1 nuORFdb_human ENST00000422154.6_1_X:102933531-102940140:- ENST00000422154.6_1_X:102933531-102940140:-|ENST00000434230.5_3_X:102933528-102939656:- MORF4L2 5' uORF 5' uORF GN=MORF4L2 5' uORF AL20221201_MKP_PDAC_01 SCP E_20221201_NO30_400nL_HLAc1_4e7_PDAC_Slot2-6_1_3524.28715.125.1 1 1 1 15.77 5.06 9.84 10.7 81.6 8 0 0 11100.0 Hydrogen 21 (F) 10 IFIADTCLSF (-) 10 FIFIADTCLS I F/I|A|D|T|C\L\S\F 80.0 8 7 5 4 0 dual b/y bias Carbamidomethylation C:Carbamidomethylation, 99.20723 1.647009 1186.583 1186.581 -0.0013 -1.1 1.2999999999999998 3.8 3488.1 nuORFdb_human ENST00000422154.6_1_X:102933531-102940140:- ENST00000422154.6_1_X:102933531-102940140:-|ENST00000434230.5_3_X:102933528-102939656:- MORF4L2 5' uORF 5' uORF GN=MORF4L2 5' uORF E_20221201_NO30_400nL_HLAc1_4e7_PDAC_Slot2-6_1_3524 1mio-rep1 E_20221201_NO30_400nL_HLAc1_1e6_directIP_titration_rep1_Slot1-06_1_3498.11782.1400.2 2 3 1 12.92 5.71 6.94 7.21 76.5 8 1 1 12300.0 Hydrogen 12 (M) 9 IFSQRSYSY (L) 9 IFHPSSDGKY I/F|S/Q/R\S\Y|S\Y 100.0 9 5 5 2 0 unbiased 40.699539 0.876566 575.7755 1150.553 -0.008 -7.0 -2.0999999999999996 8.59 3414.9 nuORFdb_human ENST00000567239.5_1_16:69602430-69660368:+ ENST00000567239.5_1_16:69602430-69660368:+ NFAT5 Out-of-Frame Out-of-Frame GN=NFAT5 Out-of-Frame E_20221201_NO30_400nL_HLAc1_1e6_directIP_titration_rep1_Slot1-06_1_3498 10mio-rep1 E_20221201_NO30_400nL_HLAc1_1e7_directIP_titration_rep1_Slot2-3_1_3512.11475.1410.2 2 1 1 13.43 7.0 6.97 6.43 73.8 8 1 1 39200.0 Hydrogen 12 (M) 9 IFSQRSYSY (L) 9 FLSQSPMNNL I/F|S/Q/R\S\Y|S\Y 100.0 9 5 5 2 0 unbiased 39.645515 0.869963 575.7755 1150.553 -0.01 -8.7 -0.9999999999999991 8.59 3414.9 nuORFdb_human ENST00000567239.5_1_16:69602430-69660368:+ ENST00000567239.5_1_16:69602430-69660368:+ NFAT5 Out-of-Frame Out-of-Frame GN=NFAT5 Out-of-Frame E_20221201_NO30_400nL_HLAc1_1e7_directIP_titration_rep1_Slot2-3_1_3512 10mio-rep2 E_20221201_NO30_400nL_HLAc1_1e7_directIP_titration_rep2_Slot2-3_1_3513.11431.1410.2 2 1 1 14.59 7.66 6.39 6.93 78.1 8 1 1 43600.0 Hydrogen 12 (M) 9 IFSQRSYSY (L) 9 IFHPSSDGKY I/F|S/Q/R\S\Y|S\Y 100.0 9 5 5 2 0 unbiased 39.492432 0.870142 575.7749 1150.553 -0.01 -8.7 -1.9999999999999991 8.59 3414.9 nuORFdb_human ENST00000567239.5_1_16:69602430-69660368:+ ENST00000567239.5_1_16:69602430-69660368:+ NFAT5 Out-of-Frame Out-of-Frame GN=NFAT5 Out-of-Frame E_20221201_NO30_400nL_HLAc1_1e7_directIP_titration_rep2_Slot2-3_1_3513 10mio-rep3 E_20221201_NO30_400nL_HLAc1_1e7_directIP_titration_rep3_Slot2-3_1_3514.11409.1414.2 2 1 1 11.47 5.92 5.63 5.55 63.3 7 1 1 31500.0 Hydrogen 12 (M) 9 IFSQRSYSY (L) 9 FIFmIDVSY I/F/S/Q/R\S Y|S\Y 77.8 7 3 5 1 0 unbiased 39.416653 0.867821 575.7773 1150.553 -0.006 -5.2 1.0999999999999996 8.59 3414.9 nuORFdb_human ENST00000567239.5_1_16:69602430-69660368:+ ENST00000567239.5_1_16:69602430-69660368:+ NFAT5 Out-of-Frame Out-of-Frame GN=NFAT5 Out-of-Frame E_20221201_NO30_400nL_HLAc1_1e7_directIP_titration_rep3_Slot2-3_1_3514 20mio-rep1 E_20221201_NO30_400nL_HLAc1_2e7_directIP_titration_rep1_Slot2-4_1_3515.11354.1406.2 2 1 1 9.15 3.61 9.15 5.55 51.5 8 1 1 42800.0 Hydrogen 12 (M) 9 IFSQRSYSY (L) 9 FLSQSPMNNL I/F|S/Q/R\S\Y|S-Y 100.0 9 4 5 2 1 unbiased 39.215996 0.872602 575.7767 1150.553 -0.006 -5.2 1.0 8.59 3414.9 nuORFdb_human ENST00000567239.5_1_16:69602430-69660368:+ ENST00000567239.5_1_16:69602430-69660368:+ NFAT5 Out-of-Frame Out-of-Frame GN=NFAT5 Out-of-Frame E_20221201_NO30_400nL_HLAc1_2e7_directIP_titration_rep1_Slot2-4_1_3515 20mio-rep2 E_20221201_NO30_400nL_HLAc1_2e7_directIP_titration_rep3_Slot2-4_1_3518.11497.1403.2 2 1 1 12.28 7.11 6.27 5.17 69.6 7 1 1 46100.0 Hydrogen 12 (M) 9 IFSQRSYSY (L) 9 FIFmIDVSY I/F|S/Q/R S\Y|S\Y 77.8 7 4 5 2 0 unbiased 39.711502 0.874224 575.7768 1150.553 -0.006 -5.2 1.0999999999999996 8.59 3414.9 nuORFdb_human ENST00000567239.5_1_16:69602430-69660368:+ ENST00000567239.5_1_16:69602430-69660368:+ NFAT5 Out-of-Frame Out-of-Frame GN=NFAT5 Out-of-Frame E_20221201_NO30_400nL_HLAc1_2e7_directIP_titration_rep3_Slot2-4_1_3518 20mio-rep3 E_20221201_NO30_400nL_HLAc1_2e7_directIP_titration_rep4_Slot2-4_1_3519.11387.1402.2 2 3 1 7.17 5.0 7.17 50.7 7 1 1 51500.0 Hydrogen 12 (M) 9 IFSQRSYSY (L) 9 I/F/S/Q/R S\Y|S\Y 77.8 7 3 5 1 0 unbiased 39.336525 0.875168 575.7769 1150.553 -0.006 -5.2 0.5999999999999996 8.59 3414.9 nuORFdb_human ENST00000567239.5_1_16:69602430-69660368:+ ENST00000567239.5_1_16:69602430-69660368:+ NFAT5 Out-of-Frame Out-of-Frame GN=NFAT5 Out-of-Frame E_20221201_NO30_400nL_HLAc1_2e7_directIP_titration_rep4_Slot2-4_1_3519 40mio-rep1 E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep1_Slot1-04_1_3496.11652.1377.2 2 1 1 12.82 6.59 6.33 6.23 63.7 7 1 1 132000.0 Hydrogen 12 (M) 9 IFSQRSYSY (L) 9 IFCTGKVYY I/F|S|Q/R S\Y|S\Y 77.8 7 5 5 3 0 dual b/y bias 40.250778 0.890975 575.7785 1150.553 -0.002 -1.8 1.4000000000000001 8.59 3414.9 nuORFdb_human ENST00000567239.5_1_16:69602430-69660368:+ ENST00000567239.5_1_16:69602430-69660368:+ NFAT5 Out-of-Frame Out-of-Frame GN=NFAT5 Out-of-Frame E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep1_Slot1-04_1_3496 40mio-rep2 E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep2_Slot2-5_1_3520.11366.1379.2 2 1 1 16.91 9.2 8.8 7.71 83.0 8 1 1 134000.0 Hydrogen 12 (M) 9 IFSQRSYSY (L) 9 FIFmIDVSY I/F|S|Q/R\S\Y|S\Y 100.0 9 6 5 3 0 dual b/y bias 39.263634 0.889243 575.7778 1150.553 -0.004 -3.5 1.4000000000000004 8.59 3414.9 nuORFdb_human ENST00000567239.5_1_16:69602430-69660368:+ ENST00000567239.5_1_16:69602430-69660368:+ NFAT5 Out-of-Frame Out-of-Frame GN=NFAT5 Out-of-Frame E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep2_Slot2-5_1_3520 40mio-rep3 E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep3_Slot2-5_1_3521.11387.1380.2 2 4 1 19.3 10.19 8.32 9.11 94.1 8 1 1 320000.0 Hydrogen 12 (M) 9 IFSQRSYSY (L) 9 FLSQSPMNNL I/F|S|Q/R\S\Y|S\Y 100.0 9 6 5 3 0 dual b/y bias 39.339348 0.888999 575.7776 1150.553 -0.004 -3.5 1.0999999999999996 8.59 3414.9 nuORFdb_human ENST00000567239.5_1_16:69602430-69660368:+ ENST00000567239.5_1_16:69602430-69660368:+ NFAT5 Out-of-Frame Out-of-Frame GN=NFAT5 Out-of-Frame E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep3_Slot2-5_1_3521 40mio-rep4 E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep4_Slot2-5_1_3522.11376.1387.2 2 2 1 11.98 5.84 6.72 6.14 64.0 8 1 1 75900.0 Hydrogen 12 (M) 9 IFSQRSYSY (L) 9 FLSQSPMNNL I/F|S/Q/R\S\Y|S\Y 100.0 9 5 5 2 0 unbiased 39.286495 0.884307 575.7777 1150.553 -0.004 -3.5 1.2000000000000002 8.59 3414.9 nuORFdb_human ENST00000567239.5_1_16:69602430-69660368:+ ENST00000567239.5_1_16:69602430-69660368:+ NFAT5 Out-of-Frame Out-of-Frame GN=NFAT5 Out-of-Frame E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep4_Slot2-5_1_3522 5mio-rep1 E_20221201_NO30_400nL_HLAc1_5e6_directIP_titration_rep1_Slot1-37_1_3502.11696.1404.2 2 1 1 13.77 5.87 7.88 7.9 67.8 8 1 1 15300.0 Hydrogen 12 (M) 9 IFSQRSYSY (L) 9 FLSQSPMNNL I/F|S|Q/R\S\Y\S\Y 100.0 9 6 4 2 0 unbiased 40.403381 0.873815 575.7757 1150.553 -0.008 -7.0 0.0 8.59 3414.9 nuORFdb_human ENST00000567239.5_1_16:69602430-69660368:+ ENST00000567239.5_1_16:69602430-69660368:+ NFAT5 Out-of-Frame Out-of-Frame GN=NFAT5 Out-of-Frame E_20221201_NO30_400nL_HLAc1_5e6_directIP_titration_rep1_Slot1-37_1_3502 5mio-rep2 E_20221201_NO30_400nL_HLAc1_5e6_directIP_titration_rep2_Slot2-2_1_3510.11651.1416.2 2 1 1 9.57 4.58 4.62 4.99 65.3 8 1 1 17900.0 Hydrogen 12 (M) 9 IFSQRSYSY (L) 9 SEAGSSRSQEI I/F/S/Q/R\S/Y\S\Y 100.0 9 3 5 0 0 unbiased 40.243164 0.866334 575.7747 1150.553 -0.01 -8.7 0.0 8.59 3414.9 nuORFdb_human ENST00000567239.5_1_16:69602430-69660368:+ ENST00000567239.5_1_16:69602430-69660368:+ NFAT5 Out-of-Frame Out-of-Frame GN=NFAT5 Out-of-Frame E_20221201_NO30_400nL_HLAc1_5e6_directIP_titration_rep2_Slot2-2_1_3510 5mio-rep3 E_20221201_NO30_400nL_HLAc1_5e6_directIP_titration_rep3_Slot2-2_1_3511.11664.1414.2 2 1 1 9.18 3.92 4.49 5.26 65.6 7 1 1 7390.0 Hydrogen 12 (M) 9 IFSQRSYSY (L) 9 KDRYGVYSY I/F/S/Q R\S/Y|S\Y 77.8 7 3 5 1 0 unbiased 40.295269 0.867278 575.7743 1150.553 -0.012 -10.5 -2.3000000000000007 8.59 3414.9 nuORFdb_human ENST00000567239.5_1_16:69602430-69660368:+ ENST00000567239.5_1_16:69602430-69660368:+ NFAT5 Out-of-Frame Out-of-Frame GN=NFAT5 Out-of-Frame E_20221201_NO30_400nL_HLAc1_5e6_directIP_titration_rep3_Slot2-2_1_3511 SCP E_20221201_NO30_400nL_HLAc1_4e7_PDAC_Slot2-6_1_3524.16138.1440.2 2 2 1 14.35 10.11 4.24 4.24 85.2 6 1 1 28600.0 Hydrogen 27 (N) 9 IFTGRNAYF (C) 9 IFLLcTLF I/F|T|G R\N A|Y\F 55.6 5 5 4 3 0 dual b/y bias 55.752907 0.851318 544.7777 1088.552 -0.0036 -3.3 -0.8999999999999999 8.75 4839.7 nuORFdb_human ENST00000423113.5_1_10:33218934-33221518:- ENST00000423113.5_1_10:33218934-33221518:- ITGB1 Out-of-Frame Out-of-Frame GN=ITGB1 Out-of-Frame E_20221201_NO30_400nL_HLAc1_4e7_PDAC_Slot2-6_1_3524 Exploris_FAIMS VH20221207_MKP_FAIMS2022E_HLAc1_4e7_PDAC.42234.42234.2 2 1 1 12.85 6.86 3.82 5.98 82.9 6 1 1 27400000.0 Hydrogen 27 (N) 9 IFTGRNAYF (C) 9 IFLLcTLF I/F|T/G R N\A/Y|F 66.7 6 3 5 2 0 unbiased 0.984 49.5 77.15 9.92 -50.0 544.7776 1088.552 -0.0045 -4.1 -1.4999999999999996 8.75 4839.7 nuORFdb_human ENST00000423113.5_1_10:33218934-33221518:- ENST00000423113.5_1_10:33218934-33221518:- ITGB1 Out-of-Frame Out-of-Frame GN=ITGB1 Out-of-Frame VH20221207_MKP_FAIMS2022E_HLAc1_4e7_PDAC 10mio-rep2 E_20221201_NO30_400nL_HLAc1_1e7_directIP_titration_rep2_Slot2-3_1_3513.25378.627.1 1 1 1 8.84 7.33 1.51 1.51 81.6 6 0 0 1340.0 Hydrogen 12 (I) 8 ILALNLGV (R) 8 LLALVQGV I L/A/L\N\L\G\V 75.0 6 4 2 0 0 b-ion bias 87.651421 1.350096 812.5153 812.524 -0.009 -11.1 -4.3999999999999995 5.52 3509.2 nuORFdb_human ENST00000379170.8_2_6:13788628-13788718:- ENST00000379170.8_2_6:13788628-13788718:- MCUR1 3' dORF 3' dORF GN=MCUR1 3' dORF E_20221201_NO30_400nL_HLAc1_1e7_directIP_titration_rep2_Slot2-3_1_3513 20mio-rep3 E_20221201_NO30_400nL_HLAc1_2e7_directIP_titration_rep4_Slot2-4_1_3519.26038.440.1 1 1 1 9.6 8.22 4.02 1.39 79.2 6 0 0 1980.0 Hydrogen 11 (L) 9 ILDEIVLAA (R) 9 LEnLLVLAA I L D\E\I\V\L\A\A 66.7 6 6 0 0 0 b-ion bias 89.929443 1.461611 956.5642 956.566 -0.0023 -2.4 3.4 3.66 2390.7 nuORFdb_human ENST00000370440.5_1_1:91447884-91486849:- ENST00000370440.5_1_1:91447884-91486849:-|ENST00000337393.9_1_1:91447884-91487590:- ZNF644 5' Overlap uORF 5' Overlap uORF GN=ZNF644 5' Overlap uORF E_20221201_NO30_400nL_HLAc1_2e7_directIP_titration_rep4_Slot2-4_1_3519 40mio-rep3 E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep3_Slot2-5_1_3521.26002.434.1 1 1 1 10.06 8.47 2.87 1.59 82.1 6 0 0 5040.0 Hydrogen 11 (L) 9 ILDEIVLAA (R) 9 LEnLLVLAA I L D\E\I\V\L\A\A 66.7 6 6 0 0 0 b-ion bias 89.819603 1.465449 956.562 956.566 -0.0043 -4.5 0.09999999999999964 3.66 2390.7 nuORFdb_human ENST00000370440.5_1_1:91447884-91486849:- ENST00000370440.5_1_1:91447884-91486849:-|ENST00000337393.9_1_1:91447884-91487590:- ZNF644 5' Overlap uORF 5' Overlap uORF GN=ZNF644 5' Overlap uORF E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep3_Slot2-5_1_3521 10mio-rep1 E_20221201_NO30_400nL_HLAc1_1e7_directIP_titration_rep1_Slot2-3_1_3512.25537.440.1 1 1 1 9.87 8.07 1.49 1.8 79.2 5 0 0 4050.0 Hydrogen 14 (F) 8 ILDSPLLY (V) 8 nLISIPIY I L D|S|P\L\L\Y 62.5 5 5 2 2 0 b-ion bias 88.20784 1.461601 933.5229 933.529 -0.0062 -6.6 1.1000000000000005 3.8 2584.0 nuORFdb_human ENST00000373129.7_1_1:36833451-36833520:- ENST00000373129.7_1_1:36833451-36833520:-|ENST00000482458.1_1_1:36826853-36833520:- STK40 5' uORF 5' uORF GN=STK40 5' uORF E_20221201_NO30_400nL_HLAc1_1e7_directIP_titration_rep1_Slot2-3_1_3512 10mio-rep2 E_20221201_NO30_400nL_HLAc1_1e7_directIP_titration_rep2_Slot2-3_1_3513.25454.439.1 1 1 1 11.76 9.8 3.51 1.96 81.0 6 0 0 2150.0 Hydrogen 14 (F) 8 ILDSPLLY (V) 8 DLLSPILY I L/D|S|P\L\L\Y 75.0 6 5 3 2 0 unbiased 87.913719 1.462272 933.5203 933.529 -0.0092 -9.8 -3.1000000000000005 3.8 2584.0 nuORFdb_human ENST00000373129.7_1_1:36833451-36833520:- ENST00000373129.7_1_1:36833451-36833520:-|ENST00000482458.1_1_1:36826853-36833520:- STK40 5' uORF 5' uORF GN=STK40 5' uORF E_20221201_NO30_400nL_HLAc1_1e7_directIP_titration_rep2_Slot2-3_1_3513 20mio-rep2 E_20221201_NO30_400nL_HLAc1_2e7_directIP_titration_rep3_Slot2-4_1_3518.25436.430.1 1 1 1 12.27 8.24 2.49 4.04 87.9 5 0 0 6590.0 Hydrogen 14 (F) 8 ILDSPLLY (V) 8 nLISIPIY I L D|S|P\L\L\Y 62.5 5 5 2 2 0 b-ion bias 87.855026 1.467491 933.5255 933.529 -0.0042 -4.5 1.7999999999999998 3.8 2584.0 nuORFdb_human ENST00000373129.7_1_1:36833451-36833520:- ENST00000373129.7_1_1:36833451-36833520:-|ENST00000482458.1_1_1:36826853-36833520:- STK40 5' uORF 5' uORF GN=STK40 5' uORF E_20221201_NO30_400nL_HLAc1_2e7_directIP_titration_rep3_Slot2-4_1_3518 20mio-rep3 E_20221201_NO30_400nL_HLAc1_2e7_directIP_titration_rep4_Slot2-4_1_3519.25460.427.1 1 1 1 9.64 7.07 1.97 2.57 83.2 5 0 0 4060.0 Hydrogen 14 (F) 8 ILDSPLLY (V) 8 nLISIPIY I L D/S|P\L\L\Y 62.5 5 4 2 1 0 b-ion bias 87.931572 1.469183 933.5218 933.529 -0.0072 -7.7 -1.9000000000000004 3.8 2584.0 nuORFdb_human ENST00000373129.7_1_1:36833451-36833520:- ENST00000373129.7_1_1:36833451-36833520:-|ENST00000482458.1_1_1:36826853-36833520:- STK40 5' uORF 5' uORF GN=STK40 5' uORF E_20221201_NO30_400nL_HLAc1_2e7_directIP_titration_rep4_Slot2-4_1_3519 40mio-rep1 E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep1_Slot1-04_1_3496.25665.409.1 1 1 1 11.33 8.43 2.15 2.91 83.0 5 0 0 16600.0 Hydrogen 14 (F) 8 ILDSPLLY (V) 8 SIIDPLIY I L D|S|P\L\L\Y 62.5 5 5 2 2 0 b-ion bias 88.66201 1.479792 933.5268 933.529 -0.0022 -2.3 0.9000000000000004 3.8 2584.0 nuORFdb_human ENST00000373129.7_1_1:36833451-36833520:- ENST00000373129.7_1_1:36833451-36833520:-|ENST00000482458.1_1_1:36826853-36833520:- STK40 5' uORF 5' uORF GN=STK40 5' uORF E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep1_Slot1-04_1_3496 40mio-rep2 E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep2_Slot2-5_1_3520.25401.425.1 1 1 1 9.42 7.5 2.31 1.92 78.0 5 0 0 13500.0 Hydrogen 14 (F) 8 ILDSPLLY (V) 8 IISnLPLY I L D/S|P\L|L\Y 62.5 5 4 3 2 0 unbiased 87.74662 1.470237 933.5228 933.529 -0.0062 -6.6 -1.6999999999999993 3.8 2584.0 nuORFdb_human ENST00000373129.7_1_1:36833451-36833520:- ENST00000373129.7_1_1:36833451-36833520:-|ENST00000482458.1_1_1:36826853-36833520:- STK40 5' uORF 5' uORF GN=STK40 5' uORF E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep2_Slot2-5_1_3520 40mio-rep3 E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep3_Slot2-5_1_3521.25406.425.1 1 2 1 12.71 8.14 4.0 4.57 85.7 5 0 0 39200.0 Hydrogen 14 (F) 8 ILDSPLLY (V) 8 SIIDPLIY I L D|S|P|L\L\Y 62.5 5 5 3 3 0 dual b/y bias 87.761955 1.47046 933.5277 933.529 -0.0012 -1.2 3.3999999999999995 3.8 2584.0 nuORFdb_human ENST00000373129.7_1_1:36833451-36833520:- ENST00000373129.7_1_1:36833451-36833520:-|ENST00000482458.1_1_1:36826853-36833520:- STK40 5' uORF 5' uORF GN=STK40 5' uORF E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep3_Slot2-5_1_3521 SCP E_20221201_NO30_400nL_HLAc1_4e7_PDAC_Slot2-6_1_3524.18679.466.1 1 1 1 8.99 5.27 2.51 3.72 64.7 7 0 0 6400.0 Hydrogen 6 (H) 9 ILGSSLCTV (-) 9 VTGLSLCSI I/L/G S/S|L|C\T\V 77.8 7 4 5 2 0 unbiased Carbamidomethylation C:Carbamidomethylation, 64.529091 1.446243 949.4992 949.502 -0.0033 -3.5 -1.1 5.52 1488.8 nuORFdb_human ENST00000495535.1_1_7:150067035-150067080:+ ENST00000495535.1_1_7:150067035-150067080:+ REPIN1 ncRNA Retained Intron lncRNA GN=REPIN1 ncRNA Retained Intron E_20221201_NO30_400nL_HLAc1_4e7_PDAC_Slot2-6_1_3524 40mio-rep1 E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep1_Slot1-04_1_3496.19964.1308.2 2 3 1 12.06 10.87 3.18 1.2 72.6 6 1 1 18500.0 M10m Hydrogen 3 (S) 9 ILMKEQLmL (S) 9 IIDIKCLmL I/L/M/K E Q|L|m\L 66.7 6 3 5 2 0 unbiased m:Oxidized methionine 68.965347 0.933668 567.8151 1118.631 15.9915 -3.0 0.20000000000000018 6.0 5535.6 nuORFdb_human ENST00000478691.5_1_1:23664326-23665089:- ENST00000478691.5_1_1:23664326-23665089:-|ENST00000490652.1_1_1:23664981-23665089:- HNRNPR Out-of-Frame Out-of-Frame GN=HNRNPR Out-of-Frame E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep1_Slot1-04_1_3496 40mio-rep2 E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep2_Slot2-5_1_3520.19730.1329.2 2 1 1 15.03 12.21 4.48 2.82 79.4 7 1 1 3970.0 M10m Hydrogen 3 (S) 9 ILMKEQLmL (S) 9 LLRLESSGmI I/L/M|K\E Q\L|m\L 77.8 7 5 4 2 0 unbiased m:Oxidized methionine 68.157928 0.920802 567.8141 1118.631 15.9895 -4.8 0.10000000000000053 6.0 5535.6 nuORFdb_human ENST00000478691.5_1_1:23664326-23665089:- ENST00000478691.5_1_1:23664326-23665089:-|ENST00000490652.1_1_1:23664981-23665089:- HNRNPR Out-of-Frame Out-of-Frame GN=HNRNPR Out-of-Frame E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep2_Slot2-5_1_3520 10mio-rep1 E_20221201_NO30_400nL_HLAc1_1e7_directIP_titration_rep1_Slot2-3_1_3512.16094.1344.2 2 2 1 13.74 10.09 3.65 3.65 73.7 6 1 1 7970.0 M5m M10m Hydrogen 3 (S) 9 ILmKEQLmL (S) 9 LYPNmTAVLL I/L/m|K E Q|L|m\L 66.7 6 4 5 3 0 dual b/y bias m:Oxidized methionine 55.596943 0.911415 575.8104 1118.631 31.9815 -7.2 0.5 6.0 5535.6 nuORFdb_human ENST00000478691.5_1_1:23664326-23665089:- ENST00000478691.5_1_1:23664326-23665089:-|ENST00000490652.1_1_1:23664981-23665089:- HNRNPR Out-of-Frame Out-of-Frame GN=HNRNPR Out-of-Frame E_20221201_NO30_400nL_HLAc1_1e7_directIP_titration_rep1_Slot2-3_1_3512 10mio-rep2 E_20221201_NO30_400nL_HLAc1_1e7_directIP_titration_rep2_Slot2-3_1_3513.16050.1340.2 2 2 1 8.56 6.34 2.75 2.22 55.7 6 1 1 7980.0 M5m M10m Hydrogen 3 (S) 9 ILmKEQLmL (S) 9 ILLEQKmmI I/L|m\K E Q/L\m\L 66.7 6 4 3 1 0 unbiased m:Oxidized methionine 55.441452 0.913745 575.8115 1118.631 31.9855 -3.7 3.0 6.0 5535.6 nuORFdb_human ENST00000478691.5_1_1:23664326-23665089:- ENST00000478691.5_1_1:23664326-23665089:-|ENST00000490652.1_1_1:23664981-23665089:- HNRNPR Out-of-Frame Out-of-Frame GN=HNRNPR Out-of-Frame E_20221201_NO30_400nL_HLAc1_1e7_directIP_titration_rep2_Slot2-3_1_3513 10mio-rep3 E_20221201_NO30_400nL_HLAc1_1e7_directIP_titration_rep3_Slot2-3_1_3514.15948.1341.2 2 2 1 12.33 8.12 3.12 4.21 74.0 7 1 1 8620.0 M5m M10m Hydrogen 3 (S) 9 ILmKEQLmL (S) 9 ILLEQKmmI I/L/m|K E\Q\L|m\L 77.8 7 5 4 2 0 unbiased m:Oxidized methionine 55.092564 0.9135 575.8129 1118.631 31.9875 -2.0 4.3 6.0 5535.6 nuORFdb_human ENST00000478691.5_1_1:23664326-23665089:- ENST00000478691.5_1_1:23664326-23665089:-|ENST00000490652.1_1_1:23664981-23665089:- HNRNPR Out-of-Frame Out-of-Frame GN=HNRNPR Out-of-Frame E_20221201_NO30_400nL_HLAc1_1e7_directIP_titration_rep3_Slot2-3_1_3514 20mio-rep1 E_20221201_NO30_400nL_HLAc1_2e7_directIP_titration_rep1_Slot2-4_1_3515.16019.1328.2 2 2 1 15.61 11.59 4.54 4.02 80.8 7 1 1 13600.0 M5m M10m Hydrogen 3 (S) 9 ILmKEQLmL (S) 9 LLmKmIEQL I/L|m|K\E Q|L\m\L 77.8 7 6 4 3 0 dual b/y bias m:Oxidized methionine 55.32391 0.921331 575.8113 1118.631 31.9835 -5.5 0.7000000000000002 6.0 5535.6 nuORFdb_human ENST00000478691.5_1_1:23664326-23665089:- ENST00000478691.5_1_1:23664326-23665089:-|ENST00000490652.1_1_1:23664981-23665089:- HNRNPR Out-of-Frame Out-of-Frame GN=HNRNPR Out-of-Frame E_20221201_NO30_400nL_HLAc1_2e7_directIP_titration_rep1_Slot2-4_1_3515 20mio-rep2 E_20221201_NO30_400nL_HLAc1_2e7_directIP_titration_rep3_Slot2-4_1_3518.16026.1325.2 2 2 1 13.99 9.26 3.44 4.72 81.2 8 1 1 9180.0 M5m M10m Hydrogen 3 (S) 9 ILmKEQLmL (S) 9 LLEQKmmIL I/L/m/K/E\Q|L\m\L 100.0 9 4 5 1 0 unbiased m:Oxidized methionine 55.35358 0.923074 575.8117 1118.631 31.9855 -3.7 2.5999999999999996 6.0 5535.6 nuORFdb_human ENST00000478691.5_1_1:23664326-23665089:- ENST00000478691.5_1_1:23664326-23665089:-|ENST00000490652.1_1_1:23664981-23665089:- HNRNPR Out-of-Frame Out-of-Frame GN=HNRNPR Out-of-Frame E_20221201_NO30_400nL_HLAc1_2e7_directIP_titration_rep3_Slot2-4_1_3518 20mio-rep3 E_20221201_NO30_400nL_HLAc1_2e7_directIP_titration_rep4_Slot2-4_1_3519.16108.1332.2 2 2 1 15.69 10.27 3.75 5.42 81.4 6 1 1 9320.0 M5m M10m Hydrogen 3 (S) 9 ILmKEQLmL (S) 9 ILLEQKmmI I/L/m|K E Q|L|m\L 66.7 6 4 5 3 0 dual b/y bias m:Oxidized methionine 55.637463 0.919184 575.8107 1118.631 31.9835 -5.5 0.2999999999999998 6.0 5535.6 nuORFdb_human ENST00000478691.5_1_1:23664326-23665089:- ENST00000478691.5_1_1:23664326-23665089:-|ENST00000490652.1_1_1:23664981-23665089:- HNRNPR Out-of-Frame Out-of-Frame GN=HNRNPR Out-of-Frame E_20221201_NO30_400nL_HLAc1_2e7_directIP_titration_rep4_Slot2-4_1_3519 40mio-rep1 E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep1_Slot1-04_1_3496.16163.1297.2 2 2 1 15.66 10.41 3.9 5.25 83.1 8 1 1 23700.0 M5m M10m Hydrogen 3 (S) 9 ILmKEQLmL (S) 9 ILLEQKmmI I/L/m/K\E\Q|L|m\L 100.0 9 5 5 2 0 unbiased m:Oxidized methionine 55.831539 0.94098 575.8124 1118.631 31.9855 -3.7 -0.5 6.0 5535.6 nuORFdb_human ENST00000478691.5_1_1:23664326-23665089:- ENST00000478691.5_1_1:23664326-23665089:-|ENST00000490652.1_1_1:23664981-23665089:- HNRNPR Out-of-Frame Out-of-Frame GN=HNRNPR Out-of-Frame E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep1_Slot1-04_1_3496 40mio-rep2 E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep2_Slot2-5_1_3520.15871.1315.2 2 2 1 15.97 10.8 3.81 5.16 82.4 7 1 1 13100.0 M5m M10m Hydrogen 3 (S) 9 ILmKEQLmL (S) 9 LYPNmTAVLL I/L/m|K E\Q|L|m\L 77.8 7 5 5 3 0 dual b/y bias m:Oxidized methionine 54.82444 0.929283 575.8116 1118.631 31.9855 -3.7 1.2000000000000002 6.0 5535.6 nuORFdb_human ENST00000478691.5_1_1:23664326-23665089:- ENST00000478691.5_1_1:23664326-23665089:-|ENST00000490652.1_1_1:23664981-23665089:- HNRNPR Out-of-Frame Out-of-Frame GN=HNRNPR Out-of-Frame E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep2_Slot2-5_1_3520 40mio-rep4 E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep4_Slot2-5_1_3522.15873.1323.2 2 4 1 13.1 8.99 2.71 4.11 80.8 6 1 1 25600.0 M5m M10m Hydrogen 3 (S) 9 ILmKEQLmL (S) 9 ILLEQKmmI I/L/m|K E Q|L|m\L 66.7 6 4 5 3 0 dual b/y bias m:Oxidized methionine 54.81773 0.924358 575.8141 1118.631 31.9895 -0.3 4.4 6.0 5535.6 nuORFdb_human ENST00000478691.5_1_1:23664326-23665089:- ENST00000478691.5_1_1:23664326-23665089:-|ENST00000490652.1_1_1:23664981-23665089:- HNRNPR Out-of-Frame Out-of-Frame GN=HNRNPR Out-of-Frame E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep4_Slot2-5_1_3522 SCP E_20221201_NO30_400nL_HLAc1_4e7_PDAC_Slot2-6_1_3524.27372.1300.2 2 1 1 11.31 5.83 4.89 5.48 82.0 8 2 2 33800.0 Hydrogen 5 (A) 9 ILPKVIYRF (N) 9 LLPKGRSALPP I/L/P/K/V/I/Y/R\F 100.0 9 1 7 0 0 y-ion bias 94.565643 0.938564 574.8623 1148.719 -0.0023 -2.0 0.3999999999999999 9.99 14570.0 nuORFdb_human ENST00000502413.2_1_1:67131896-67132262:- ENST00000502413.2_1_1:67131896-67132262:-|T266277_4:72486062-72486887:+ RP4-598P13.1 Antisense lncRNA GN=RP4-598P13.1 Antisense E_20221201_NO30_400nL_HLAc1_4e7_PDAC_Slot2-6_1_3524 Exploris_FAIMS VH20221207_MKP_FAIMS2022E_HLAc1_4e7_PDAC.60984.60984.3 3 1 1 11.51 5.36 11.51 6.15 72.5 6 2 2 4640000.0 Hydrogen 5 (A) 9 ILPKVIYRF (N) 9 LLPKGRSALPP I L|P/K/V/I/Y R/F 55.6 5 1 6 1 0 y-ion bias 0.98 78.9 105.32 88.89 -50.0 383.5769 1148.719 -0.003 -2.6 0.0 9.99 14570.0 nuORFdb_human ENST00000502413.2_1_1:67131896-67132262:- ENST00000502413.2_1_1:67131896-67132262:-|T266277_4:72486062-72486887:+ RP4-598P13.1 Antisense lncRNA GN=RP4-598P13.1 Antisense VH20221207_MKP_FAIMS2022E_HLAc1_4e7_PDAC Eva-50mio-3fr AL20210827_EV_HLA_PDAC_PANFRO413_2D_50e6_W632_classI_fxn3.49475.49475.2 2 1 1 13.07 9.66 3.41 3.41 93.1 5 0 0 7870000.0 Hydrogen 17 (M) 9 ILPPTSYIF (-) 9 PSPYTLILF I L|P P T-S\Y|I/F 44.4 4 3 3 2 1 unbiased 0.786 66.0 88.9 84.08 0.0 525.7968 1050.587 -0.0007 -0.7 -1.2 5.52 2614.1 nuORFdb_human ENST00000473764.5_1_X:48755552-48755627:+ ENST00000473764.5_1_X:48755552-48755627:+ PQBP1 5' Overlap uORF 5' Overlap uORF GN=PQBP1 5' Overlap uORF AL20210827_EV_HLA_PDAC_PANFRO413_2D_50e6_W632_classI_fxn3 Exploris AL20221201_MKP_PDAC_01.65895.65895.2 2 1 1 10.6 7.61 3.0 3.0 70.0 5 0 0 140000000.0 Hydrogen 17 (M) 9 ILPPTSYIF (-) 9 PSPYTLILF I L|P P T-S\Y|I/F 44.4 4 3 3 2 1 unbiased 0.902 59.6 99.12 6.11 0.0 525.798 1050.587 0.0017 1.6 0.8 5.52 2614.1 nuORFdb_human ENST00000473764.5_1_X:48755552-48755627:+ ENST00000473764.5_1_X:48755552-48755627:+ PQBP1 5' Overlap uORF 5' Overlap uORF GN=PQBP1 5' Overlap uORF AL20221201_MKP_PDAC_01 SCP E_20221201_NO30_400nL_HLAc1_4e7_PDAC_Slot2-6_1_3524.28613.288.1 1 1 1 12.29 6.85 4.4 5.44 94.8 7 0 0 24500.0 Hydrogen 17 (M) 9 ILPPTSYIF (-) 9 LISTLmPIF I L/P/P\T-S\Y\I\F 77.8 7 4 2 0 1 b-ion bias 98.855034 1.551232 1050.5849 1050.587 -0.007 -6.7 -4.300000000000001 5.52 2614.1 nuORFdb_human ENST00000473764.5_1_X:48755552-48755627:+ ENST00000473764.5_1_X:48755552-48755627:+ PQBP1 5' Overlap uORF 5' Overlap uORF GN=PQBP1 5' Overlap uORF E_20221201_NO30_400nL_HLAc1_4e7_PDAC_Slot2-6_1_3524 SCP E_20221201_NO30_400nL_HLAc1_4e7_PDAC_Slot2-6_1_3524.25468.366.1 1 3 1 9.83 6.22 3.35 3.61 76.8 7 0 0 86100.0 Hydrogen 6 (E) 9 ILPSTTLTF (L) 9 LInAISLTF I/L/P S/T-T\L\T\F 77.8 7 3 3 0 1 unbiased 87.983879 1.505636 992.5664 992.566 -0.0003 -0.3 2.1 5.52 1793.1 nuORFdb_human ENST00000519683.1_1_5:153830726-153830774:+ ENST00000519683.1_1_5:153830726-153830774:+|ENST00000297109.10_1_5:153830684-153832986:+ SAP30L ncRNA Retained Intron lncRNA GN=SAP30L ncRNA Retained Intron E_20221201_NO30_400nL_HLAc1_4e7_PDAC_Slot2-6_1_3524 SCP E_20221201_NO30_400nL_HLAc1_4e7_PDAC_Slot2-6_1_3524.30318.486.1 1 1 1 13.09 11.97 1.29 1.12 90.7 6 0 0 220000.0 Hydrogen 89 (G) 8 ILPVTVLL (T) 8 LLPVLTVL I/L P|V\T\V\L\L 75.0 6 5 2 1 0 b-ion bias 104.746544 1.434265 867.5911 867.591 -0.0004 -0.4 2.0 5.52 14714.5 nuORFdb_human ENST00000552265.2_1_12:111822376-111823062:+ ENST00000552265.2_1_12:111822376-111823062:+ HSPA8P14 Pseudogene Pseudogene GN=HSPA8P14 Pseudogene E_20221201_NO30_400nL_HLAc1_4e7_PDAC_Slot2-6_1_3524 SCP E_20221201_NO30_400nL_HLAc1_4e7_PDAC_Slot2-6_1_3524.29927.309.1 1 1 1 10.47 8.5 4.49 1.96 79.9 6 0 0 12700.0 Hydrogen 14 (K) 9 ILSSLQVLL (Y) 9 IVQSLLSLL I L S\S\L/Q\V\L\L 66.7 6 5 1 0 0 b-ion bias 103.396461 1.539244 985.6231 985.629 -0.0062 -6.3 -3.9 5.52 5582.6 nuORFdb_human ENST00000609841.1_1_10:42833186-42833330:- ENST00000609841.1_1_10:42833186-42833330:- RP11-313J2.1 Pseudogene Pseudogene GN=RP11-313J2.1 Pseudogene E_20221201_NO30_400nL_HLAc1_4e7_PDAC_Slot2-6_1_3524 40mio-rep1 E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep1_Slot1-04_1_3496.24999.338.1 1 1 1 9.46 3.0 6.74 6.46 78.2 7 0 0 5280.0 Hydrogen 13 (P) 9 ILWGGEQSW (S) 9 LLGEGQEGWS I L/W/G/G\E\Q\S\W 77.8 7 4 3 0 0 unbiased 86.361908 1.521892 1075.5167 1075.521 -0.0007 -0.7 2.5 4.0 2861.3 nuORFdb_human ENST00000460115.5_1_17:41292066-41292147:+ ENST00000460115.5_1_17:41292066-41292147:+ NBR2 Pseudogene Pseudogene GN=NBR2 Pseudogene E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep1_Slot1-04_1_3496 SCP E_20221201_NO30_400nL_HLAc1_4e7_PDAC_Slot2-6_1_3524.30176.267.1 1 2 1 11.73 4.79 4.32 6.94 85.3 7 0 0 20400.0 Hydrogen 16 (M) 9 ILWPVEITL (-) 9 WPEVLLLLT I L/W|P\V\E\I\T-L 77.8 7 5 2 1 1 b-ion bias 104.256226 1.563915 1083.6416 1083.645 -0.0049 -4.5 -2.1 4.0 2842.4 nuORFdb_human ENST00000372348.6_1_9:133729537-133729609:+ ENST00000372348.6_1_9:133729537-133729609:+ ABL1 Out-of-Frame Out-of-Frame GN=ABL1 Out-of-Frame E_20221201_NO30_400nL_HLAc1_4e7_PDAC_Slot2-6_1_3524 40mio-rep1 E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep1_Slot1-04_1_3496.29930.261.1 1 4 1 15.74 5.16 6.6 10.58 90.5 7 0 0 74000.0 Hydrogen 16 (M) 9 ILWPVEITL (-) 9 GLKNInIPTL I L/W|P\V|E\I\T\L 77.8 7 6 3 2 0 b-ion bias 103.398293 1.567123 1083.6439 1083.645 -0.0049 -4.5 -1.2999999999999998 4.0 2842.4 nuORFdb_human ENST00000372348.6_1_9:133729537-133729609:+ ENST00000372348.6_1_9:133729537-133729609:+ ABL1 Out-of-Frame Out-of-Frame GN=ABL1 Out-of-Frame E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep1_Slot1-04_1_3496 40mio-rep2 E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep2_Slot2-5_1_3520.29881.262.1 1 2 1 11.78 6.32 2.94 5.46 86.0 7 0 0 24800.0 Hydrogen 16 (M) 9 ILWPVEITL (-) 9 GLKNInIPTL I L/W|P\V-E\I\T\L 77.8 7 5 2 1 1 b-ion bias 103.224045 1.566698 1083.6428 1083.645 -0.0049 -4.5 0.40000000000000036 4.0 2842.4 nuORFdb_human ENST00000372348.6_1_9:133729537-133729609:+ ENST00000372348.6_1_9:133729537-133729609:+ ABL1 Out-of-Frame Out-of-Frame GN=ABL1 Out-of-Frame E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep2_Slot2-5_1_3520 40mio-rep3 E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep3_Slot2-5_1_3521.29882.1405.2 2 3 1 13.96 7.23 4.31 6.73 82.1 7 0 0 22800.0 Hydrogen 16 (M) 9 ILWPVEITL (-) 9 LDIWPLITL I L/W|P\V/E|I|T\L 77.8 7 5 5 3 0 dual b/y bias 103.219269 0.873035 542.3245 1083.645 -0.0021 -2.0 2.5999999999999996 4.0 2842.4 nuORFdb_human ENST00000372348.6_1_9:133729537-133729609:+ ENST00000372348.6_1_9:133729537-133729609:+ ABL1 Out-of-Frame Out-of-Frame GN=ABL1 Out-of-Frame E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep3_Slot2-5_1_3521 40mio-rep4 E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep4_Slot2-5_1_3522.29891.259.1 1 3 1 10.12 5.26 2.59 4.86 81.0 6 0 0 35000.0 Hydrogen 16 (M) 9 ILWPVEITL (-) 9 WPEVLLLLT I L/W|P\V E\I\T\L 55.6 5 5 2 1 0 b-ion bias 103.240555 1.568703 1083.6413 1083.645 -0.0049 -4.5 0.20000000000000018 4.0 2842.4 nuORFdb_human ENST00000372348.6_1_9:133729537-133729609:+ ENST00000372348.6_1_9:133729537-133729609:+ ABL1 Out-of-Frame Out-of-Frame GN=ABL1 Out-of-Frame E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep4_Slot2-5_1_3522 40mio-rep1 E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep1_Slot1-04_1_3496.15447.1392.2 2 1 1 9.01 5.1 2.82 3.91 68.6 6 1 1 12700.0 Hydrogen 39 (N) 9 IMGEFRTEV (-) 9 VVmETnKML I/M/G/E|F/R T E\V 66.7 6 2 5 1 0 y-ion bias 53.358658 0.881312 541.269 1081.535 -0.0039 -3.6 -0.3999999999999999 4.53 5756.7 nuORFdb_human ENST00000534336.1_1_11:65268195-65268336:+ ENST00000534336.1_1_11:65268195-65268336:+ MALAT1 lincRNA lncRNA GN=MALAT1 lincRNA E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep1_Slot1-04_1_3496 Exploris_FAIMS VH20221207_MKP_FAIMS2022E_HLAc1_4e7_PDAC.47913.47913.2 2 1 1 9.5 8.33 1.8 1.17 76.8 7 1 1 1360000000.0 Hydrogen 12 (V) 9 IPAFERTPF (-) 9 IPAHTPTPPF I/P|A/F/E R-T/P-F 77.8 7 1 5 1 2 y-ion bias 0.821 70.6 86.1 120.0 -70.0 539.2876 1077.573 -0.0048 -4.4 -1.8000000000000003 6.0 2398.8 nuORFdb_human ENST00000424596.1_1_7:56118831-56118894:- ENST00000424596.1_1_7:56118831-56118894:- PSPH 5' uORF 5' uORF GN=PSPH 5' uORF VH20221207_MKP_FAIMS2022E_HLAc1_4e7_PDAC Eva-50mio-3fr AL20210827_EV_HLA_PDAC_PANFRO413_2D_50e6_W632_classI_fxn1.21005.21005.2 2 1 1 13.87 11.44 3.85 2.43 91.2 7 0 0 5950000.0 Hydrogen 13 (R) 9 IPAPDHPAL (R) 9 IPAnHPPLA I/P|A/P D/H|P-A-L 77.8 7 2 5 2 2 y-ion bias 0.998 100.0 39.23 100.0 0.0 465.7558 930.504 0.0 0.0 -0.1 5.08 9066.4 nuORFdb_human ENST00000440177.6_1_19:42753452-42753692:- ENST00000440177.6_1_19:42753452-42753692:- ERF Out-of-Frame Out-of-Frame GN=ERF Out-of-Frame AL20210827_EV_HLA_PDAC_PANFRO413_2D_50e6_W632_classI_fxn1 Exploris_FAIMS VH20221207_MKP_FAIMS2022E_HLAc1_4e7_PDAC.32723.32861.2 2 1 1 13.17 11.11 4.06 2.06 89.7 7 0 0 16200000.0 Hydrogen 13 (R) 9 IPAPDHPAL (R) 9 IPAnHPPLA I/P|A/P D/H|P-A-L 77.8 7 2 5 2 2 y-ion bias 0.999 78.8 62.88 52.28 -70.0 465.7544 930.504 -0.0027 -2.9 -0.2999999999999998 5.08 9066.4 nuORFdb_human ENST00000440177.6_1_19:42753452-42753692:- ENST00000440177.6_1_19:42753452-42753692:- ERF Out-of-Frame Out-of-Frame GN=ERF Out-of-Frame VH20221207_MKP_FAIMS2022E_HLAc1_4e7_PDAC SCP E_20221201_NO30_400nL_HLAc1_4e7_PDAC_Slot2-6_1_3524.16389.458.1 1 1 1 8.49 2.95 5.54 5.54 73.5 7 0 0 5410.0 Hydrogen 16 (S) 9 IPCQVGATL (-) 9 LCPQVTLQ I/P C/Q\V\G\A\T\L 77.8 7 5 2 0 0 b-ion bias Carbamidomethylation C:Carbamidomethylation, 56.619572 1.450999 958.499 958.503 -0.0036 -3.8 -1.4 5.52 2647.1 nuORFdb_human ENST00000330537.6_16:18553582-18553989:- ENST00000330537.6_16:18553582-18553989:- NOMO2 Out-of-Frame Out-of-Frame GN=NOMO2 Out-of-Frame E_20221201_NO30_400nL_HLAc1_4e7_PDAC_Slot2-6_1_3524 SCP E_20221201_NO30_400nL_HLAc1_4e7_PDAC_Slot2-6_1_3524.17150.1312.2 2 1 1 14.38 8.24 6.84 6.14 81.0 6 0 0 7420.0 Hydrogen 45 (C) 10 IPFHDPTCVY (-) 10 IWFVcSLVY I/P F H\D|P T|C|V\Y 50.0 5 5 4 3 0 unbiased Carbamidomethylation C:Carbamidomethylation, 59.248196 0.931327 624.7881 1248.572 -0.003 -2.4 0.0 5.08 6249.8 nuORFdb_human ENST00000569565.1_1_15:65899662-65903067:- ENST00000569565.1_1_15:65899662-65903067:-|ENST00000313182.6_1_15:65899662-65903458:-|ENST00000567923.1_1_15:65899662-65903535:- INTS14 3' Overlap dORF 3' Overlap dORF GN=INTS14 3' Overlap dORF E_20221201_NO30_400nL_HLAc1_4e7_PDAC_Slot2-6_1_3524 Exploris_FAIMS VH20221207_MKP_FAIMS2022E_HLAc1_4e7_PDAC.62755.62755.2 2 1 1 10.09 4.53 3.79 5.56 71.3 7 0 0 2790000.0 Hydrogen 28 (I) 9 IPFVMDPQL (Q) 9 IIPFVMDPQ I P\F\V\M\D\P-Q/L 77.8 7 5 1 0 1 b-ion bias 0.958 70.1 107.7 57.78 -70.0 530.2789 1059.554 -0.0039 -3.6 -1.0 3.8 17668.9 nuORFdb_human ENST00000412609.5_1_19:40170059-40174276:+ ENST00000412609.5_1_19:40170059-40174276:+|ENST00000598164.2_1_19:40172096-40174784:+ LGALS17A Pseudogene Pseudogene GN=LGALS17A Pseudogene VH20221207_MKP_FAIMS2022E_HLAc1_4e7_PDAC Eva-50mio-3fr AL20210827_EV_HLA_PDAC_PANFRO413_2D_50e6_W632_classI_fxn3.37454.37454.2 2 2 1 10.13 4.86 4.24 5.28 56.0 7 0 0 74500000.0 Hydrogen 3 (K) 9 IPLHFSTAF (S) 9 FTHLPSLAF I/P L/H\F\S-T/A|F 77.8 7 3 4 1 1 unbiased 0.984 86.9 68.25 46.93 0.0 516.7796 1032.551 0.0007 0.6 0.09999999999999998 6.74 1882.2 nuORFdb_human ENST00000392750.6_2_3:167437948-167437999:- ENST00000392750.6_2_3:167437948-167437999:- PDCD10 5' uORF 5' uORF GN=PDCD10 5' uORF AL20210827_EV_HLA_PDAC_PANFRO413_2D_50e6_W632_classI_fxn3 SCP E_20221201_NO30_400nL_HLAc1_4e7_PDAC_Slot2-6_1_3524.26296.1453.2 2 1 1 12.33 9.49 2.84 2.84 84.0 6 0 0 9940.0 Hydrogen 3 (K) 9 IPLHFSTAF (S) 9 LSIFPHTAF I/P/L H F\S\T\A\F 66.7 6 4 2 0 0 b-ion bias 90.844254 0.843186 516.7775 1032.551 -0.0046 -4.4 -2.0000000000000004 6.74 1882.2 nuORFdb_human ENST00000392750.6_2_3:167437948-167437999:- ENST00000392750.6_2_3:167437948-167437999:- PDCD10 5' uORF 5' uORF GN=PDCD10 5' uORF E_20221201_NO30_400nL_HLAc1_4e7_PDAC_Slot2-6_1_3524 Exploris AL20221201_MKP_PDAC_01.67009.67009.2 2 2 1 14.01 10.67 10.7 3.35 81.6 8 0 0 233000000.0 Hydrogen 3 (E) 11 IPLSYLADLTY (L) 11 PLSYLADLTYL I P\L-S-Y\L\A\D\L|T/Y 81.8 9 6 2 1 2 b-ion bias 0.982 100.0 100.68 45.77 0.0 634.8429 1268.677 0.0012 1.0 0.19999999999999996 3.8 6341.3 nuORFdb_human ENST00000472644.6_3_3:105238940-105243229:+ ENST00000472644.6_3_3:105238940-105243229:+ ALCAM Out-of-Frame Out-of-Frame GN=ALCAM Out-of-Frame AL20221201_MKP_PDAC_01 SCP E_20221201_NO30_400nL_HLAc1_4e7_PDAC_Slot2-6_1_3524.29160.65.1 1 1 1 11.98 2.23 9.78 9.75 83.1 7 0 0 2660.0 Hydrogen 3 (E) 11 IPLSYLADLTY (L) 11 YPLTVASKSTTT I P L S/Y|L\A\D\L\T\Y 63.6 7 6 2 1 0 b-ion bias 100.746811 1.681487 1268.6841 1268.677 0.0027 2.1 4.5 3.8 6341.3 nuORFdb_human ENST00000472644.6_3_3:105238940-105243229:+ ENST00000472644.6_3_3:105238940-105243229:+ ALCAM Out-of-Frame Out-of-Frame GN=ALCAM Out-of-Frame E_20221201_NO30_400nL_HLAc1_4e7_PDAC_Slot2-6_1_3524 Eva-50mio-3fr AL20210827_EV_HLA_PDAC_PANFRO413_2D_50e6_W632_classI_fxn2.31605.31605.2 2 1 1 12.62 10.93 0.53 1.69 81.9 5 1 1 3390000.0 Hydrogen 3 (R) 8 IPRLSELL (R) 8 IPRLSELL|LPRLSELL LPEISRIL I/P|R L S\E\L|L 62.5 5 4 3 2 0 unbiased 0.968 90.1 58.15 100.0 0.0 470.795 940.583 0.0001 0.1 -0.19999999999999998 6.0 9254.5 nuORFdb_human ENST00000475869.2_1_1:21766682-21767355:+ ENST00000475869.2_1_1:21766682-21767355:+|ENST00000454000.6_1_1:21766682-21771446:+|ENST00000557373.1_1_14:35343971-35344544:+ NBPF3 ncRNA Processed Transcript lncRNA GN=NBPF3 ncRNA Processed Transcript AL20210827_EV_HLA_PDAC_PANFRO413_2D_50e6_W632_classI_fxn2 Exploris_FAIMS VH20221207_MKP_FAIMS2022E_HLAc1_4e7_PDAC.46841.46841.2 2 1 1 15.57 12.24 3.64 3.33 81.0 7 1 1 11300000.0 Hydrogen 3 (R) 8 IPRLSELL (R) 8 IPRLSELL|LPRLSELL LPLRVTEL I/P|R\L\S\E\L|L 100.0 8 6 3 2 0 b-ion bias 0.912 100.0 83.95 44.01 -50.0 470.794 940.583 -0.0019 -2.0 0.6000000000000001 6.0 9254.5 nuORFdb_human ENST00000475869.2_1_1:21766682-21767355:+ ENST00000475869.2_1_1:21766682-21767355:+|ENST00000454000.6_1_1:21766682-21771446:+|ENST00000557373.1_1_14:35343971-35344544:+ NBPF3 ncRNA Processed Transcript lncRNA GN=NBPF3 ncRNA Processed Transcript VH20221207_MKP_FAIMS2022E_HLAc1_4e7_PDAC Eva-50mio-3fr AL20210827_EV_HLA_PDAC_PANFRO413_2D_50e6_W632_classI_fxn3.53289.53289.2 2 1 1 14.63 9.02 6.56 5.61 84.2 8 1 1 2210000.0 Hydrogen 41 (Y) 9 IPSFLKIVL (Q) 9 PSLFVKILI I/P|S/F\L/K\I/V/L 100.0 9 3 6 1 0 y-ion bias 0.972 94.7 95.93 100.0 0.0 515.34 1029.671 0.0021 2.0 1.5 8.75 6310.9 nuORFdb_human ENST00000379607.9_1_X:20146365-20150302:- ENST00000379607.9_1_X:20146365-20150302:- EIF1AX 3' Overlap dORF 3' Overlap dORF GN=EIF1AX 3' Overlap dORF AL20210827_EV_HLA_PDAC_PANFRO413_2D_50e6_W632_classI_fxn3 Exploris AL20221201_MKP_PDAC_01.67200.67200.2 2 1 1 17.52 8.59 6.47 8.93 94.6 8 1 1 28200000.0 Hydrogen 41 (Y) 9 IPSFLKIVL (Q) 9 LKPSLFLVL I/P|S/F\L/K\I|V/L 100.0 9 4 6 2 0 unbiased 0.886 90.7 100.92 82.38 0.0 515.3396 1029.671 0.0012 1.2 0.3999999999999999 8.75 6310.9 nuORFdb_human ENST00000379607.9_1_X:20146365-20150302:- ENST00000379607.9_1_X:20146365-20150302:- EIF1AX 3' Overlap dORF 3' Overlap dORF GN=EIF1AX 3' Overlap dORF AL20221201_MKP_PDAC_01 SCP E_20221201_NO30_400nL_HLAc1_4e7_PDAC_Slot2-6_1_3524.29514.203.1 1 1 1 10.66 5.71 4.4 4.95 79.2 7 1 1 4840.0 Hydrogen 41 (Y) 9 IPSFLKIVL (Q) 9 VITMIKIVL I/P S/F|L|K\I\V\L 77.8 7 5 4 2 0 unbiased 101.969757 1.601472 1029.6721 1029.671 -0.0007 -0.7 1.7 8.75 6310.9 nuORFdb_human ENST00000379607.9_1_X:20146365-20150302:- ENST00000379607.9_1_X:20146365-20150302:- EIF1AX 3' Overlap dORF 3' Overlap dORF GN=EIF1AX 3' Overlap dORF E_20221201_NO30_400nL_HLAc1_4e7_PDAC_Slot2-6_1_3524 Exploris_FAIMS VH20221207_MKP_FAIMS2022E_HLAc1_4e7_PDAC.65050.65084.2 2 1 1 16.43 7.13 5.88 9.3 92.5 8 1 1 14600000.0 Hydrogen 41 (Y) 9 IPSFLKIVL (Q) 9 VFLPSKIIL I/P|S/F\L/K\I|V/L 100.0 9 4 6 2 0 unbiased 0.998 96.3 111.0 55.61 -70.0 515.3372 1029.671 -0.0035 -3.4 -0.7999999999999998 8.75 6310.9 nuORFdb_human ENST00000379607.9_1_X:20146365-20150302:- ENST00000379607.9_1_X:20146365-20150302:- EIF1AX 3' Overlap dORF 3' Overlap dORF GN=EIF1AX 3' Overlap dORF VH20221207_MKP_FAIMS2022E_HLAc1_4e7_PDAC 40mio-rep1 E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep1_Slot1-04_1_3496.8659.1619.3 3 1 1 11.96 9.75 2.83 2.21 72.9 7 2 2 52700.0 Hydrogen 4 (E) 9 IPVEVKIKI (Q) 9 IVPEVLKKI I/P/V|E|V-K I/K\I 77.8 7 3 5 2 1 unbiased 29.915985 0.738408 346.9004 1038.692 -0.0067 -6.4 -3.2 8.59 5446.5 nuORFdb_human ENST00000425941.6_1_2:38972287-38975206:- ENST00000425941.6_1_2:38972287-38975206:-|ENST00000443213.5_1_2:38972287-38975206:-|ENST00000446327.6_2_2:38972287-38973889:- SRSF7 3' dORF 3' dORF GN=SRSF7 3' dORF E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep1_Slot1-04_1_3496 40mio-rep4 E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep4_Slot2-5_1_3522.8375.1630.3 3 1 1 8.5 6.21 2.13 2.29 51.1 7 2 2 14700.0 Hydrogen 4 (E) 9 IPVEVKIKI (Q) 9 IVPEVLKKI I/P/V|E|V K-I/K-I 77.8 7 2 5 2 2 y-ion bias 28.927299 0.731215 346.8994 1038.692 -0.0097 -9.3 -4.6000000000000005 8.59 5446.5 nuORFdb_human ENST00000425941.6_1_2:38972287-38975206:- ENST00000425941.6_1_2:38972287-38975206:-|ENST00000443213.5_1_2:38972287-38975206:-|ENST00000446327.6_2_2:38972287-38973889:- SRSF7 3' dORF 3' dORF GN=SRSF7 3' dORF E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep4_Slot2-5_1_3522 Eva-50mio-3fr AL20210827_EV_HLA_PDAC_PANFRO413_2D_50e6_W632_classI_fxn2.27989.27989.2 2 1 1 10.14 4.59 6.08 5.55 72.2 6 0 0 27200000.0 Hydrogen 6 (A) 9 IPVSSVIHY (-) 9 LPVHFTSLT I/P|V/S S V|I/H-Y 66.7 6 2 5 2 1 y-ion bias 0.97 57.7 51.32 50.49 0.0 507.7836 1014.562 -0.002 -2.0 -2.3 6.74 1445.7 nuORFdb_human ENST00000549645.5_1_12:53400267-53402412:+ ENST00000549645.5_1_12:53400267-53402412:+ EIF4B ncRNA Processed Transcript lncRNA GN=EIF4B ncRNA Processed Transcript AL20210827_EV_HLA_PDAC_PANFRO413_2D_50e6_W632_classI_fxn2 Exploris AL20221201_MKP_PDAC_01.36842.36842.2 2 1 1 7.18 1.19 4.72 5.99 54.7 6 0 0 39600000.0 Hydrogen 6 (A) 9 IPVSSVIHY (-) 9 LPVNPNFTL I/P|V/S S V|I/H-Y 66.7 6 2 5 2 1 y-ion bias 0.897 48.4 56.87 17.75 0.0 507.7852 1014.562 0.0012 1.2 0.3999999999999999 6.74 1445.7 nuORFdb_human ENST00000549645.5_1_12:53400267-53402412:+ ENST00000549645.5_1_12:53400267-53402412:+ EIF4B ncRNA Processed Transcript lncRNA GN=EIF4B ncRNA Processed Transcript AL20221201_MKP_PDAC_01 Exploris_FAIMS VH20221207_MKP_FAIMS2022E_HLAc1_4e7_PDAC.42680.42680.2 2 1 1 15.39 5.08 11.46 10.32 92.3 8 0 0 43500000.0 Hydrogen 6 (A) 9 IPVSSVIHY (-) 9 LVPSVLHSY I/P|V/S/S-V|I/H-Y 100.0 9 2 6 2 2 y-ion bias 0.965 94.2 77.82 4.41 -70.0 507.7835 1014.562 -0.0022 -2.1 0.5 6.74 1445.7 nuORFdb_human ENST00000549645.5_1_12:53400267-53402412:+ ENST00000549645.5_1_12:53400267-53402412:+ EIF4B ncRNA Processed Transcript lncRNA GN=EIF4B ncRNA Processed Transcript VH20221207_MKP_FAIMS2022E_HLAc1_4e7_PDAC Exploris_FAIMS VH20221207_MKP_FAIMS2022E_HLAc1_4e7_PDAC.41019.41019.2 2 1 1 7.16 5.69 3.7 1.48 63.3 7 0 0 8330000.0 Hydrogen 35 (Q) 10 IPYVQQPHPS (L) 10 LPYVADRQGF I/P|Y/V/Q-Q-P-H P S 70.0 7 1 4 1 3 y-ion bias 0.92 79.2 74.67 30.81 -50.0 583.3021 1165.6 -0.0032 -2.7 -0.10000000000000009 6.74 6791.7 nuORFdb_human ENST00000476565.1_1_1:156506896-156507079:- ENST00000476565.1_1_1:156506896-156507079:- IQGAP3 ncRNA Processed Transcript lncRNA GN=IQGAP3 ncRNA Processed Transcript VH20221207_MKP_FAIMS2022E_HLAc1_4e7_PDAC 20mio-rep2 E_20221201_NO30_400nL_HLAc1_2e7_directIP_titration_rep3_Slot2-4_1_3518.11341.758.1 1 1 1 9.81 8.19 3.99 1.62 69.3 6 0 0 2180.0 Hydrogen 29 (W) 8 IQPPPAAA (P) 8 LQPPAPAA I Q/P|P|P\A\A\A 75.0 6 5 3 2 0 unbiased 39.173077 1.271265 764.4295 764.43 -0.0011 -1.5 4.8 5.52 5568.2 smORF_v4|nuORFdb_human GSK3B_3:119812878-119813036:- GSK3B_3:119812878-119813036:-|ENST00000264235.12_2_3:119812880-119813177:- GSK3B uORF 3:119812878-119813036:- 3 GSK3B 5' uORF 5' uORF GN=GSK3B E_20221201_NO30_400nL_HLAc1_2e7_directIP_titration_rep3_Slot2-4_1_3518 40mio-rep1 E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep1_Slot1-04_1_3496.11501.741.1 1 3 1 9.83 7.55 2.78 2.28 68.1 6 0 0 12000.0 Hydrogen 29 (W) 8 IQPPPAAA (P) 8 GALPPAPAA I Q/P|P|P-A\A\A 75.0 6 4 3 2 1 unbiased 39.72887 1.281462 764.428 764.43 -0.0021 -2.8 0.40000000000000036 5.52 5568.2 smORF_v4|nuORFdb_human GSK3B_3:119812878-119813036:- GSK3B_3:119812878-119813036:-|ENST00000264235.12_2_3:119812880-119813177:- GSK3B uORF 3:119812878-119813036:- 3 GSK3B 5' uORF 5' uORF GN=GSK3B E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep1_Slot1-04_1_3496 40mio-rep2 E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep2_Slot2-5_1_3520.11216.755.1 1 1 1 7.45 5.75 2.2 1.7 60.5 6 0 0 3080.0 Hydrogen 29 (W) 8 IQPPPAAA (P) 8 GALPPAPAA I Q/P/P|P\A\A\A 75.0 6 4 3 1 0 unbiased 38.745872 1.27284 764.4257 764.43 -0.0041 -5.4 -0.5 5.52 5568.2 smORF_v4|nuORFdb_human GSK3B_3:119812878-119813036:- GSK3B_3:119812878-119813036:-|ENST00000264235.12_2_3:119812880-119813177:- GSK3B uORF 3:119812878-119813036:- 3 GSK3B 5' uORF 5' uORF GN=GSK3B E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep2_Slot2-5_1_3520 40mio-rep3 E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep3_Slot2-5_1_3521.11221.725.1 1 2 1 9.65 7.07 2.39 2.57 69.2 6 0 0 6760.0 Hydrogen 29 (W) 8 IQPPPAAA (P) 8 GALPPAPAA I Q/P|P|P-A\A\A 75.0 6 4 3 2 1 unbiased 38.765743 1.29109 764.4261 764.43 -0.0041 -5.4 -0.8000000000000007 5.52 5568.2 smORF_v4|nuORFdb_human GSK3B_3:119812878-119813036:- GSK3B_3:119812878-119813036:-|ENST00000264235.12_2_3:119812880-119813177:- GSK3B uORF 3:119812878-119813036:- 3 GSK3B 5' uORF 5' uORF GN=GSK3B E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep3_Slot2-5_1_3521 40mio-rep4 E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep4_Slot2-5_1_3522.11226.753.1 1 2 1 9.18 7.54 1.91 1.64 68.1 6 0 0 7230.0 Hydrogen 29 (W) 8 IQPPPAAA (P) 8 LQPPAPAA I Q/P|P|P\A\A\A 75.0 6 5 3 2 0 unbiased 38.768764 1.274451 764.4244 764.43 -0.0061 -8.0 -3.3 5.52 5568.2 smORF_v4|nuORFdb_human GSK3B_3:119812878-119813036:- GSK3B_3:119812878-119813036:-|ENST00000264235.12_2_3:119812880-119813177:- GSK3B uORF 3:119812878-119813036:- 3 GSK3B 5' uORF 5' uORF GN=GSK3B E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep4_Slot2-5_1_3522 20mio-rep3 E_20221201_NO30_400nL_HLAc1_2e7_directIP_titration_rep4_Slot2-4_1_3519.10572.1689.3 3 1 1 9.51 3.29 6.07 6.22 72.4 6 1 1 3040.0 Hydrogen 87 (Q) 9 IRFPGDHSV (A) 9 RIFYSENV I R/F|P/G\D-H S\V 55.6 5 3 3 1 1 unbiased 36.522942 0.693586 343.1792 1027.532 -0.0095 -9.3 -3.500000000000001 6.74 15609.4 nuORFdb_human ENST00000551108.1_1_12:29542438-29639896:+ ENST00000551108.1_1_12:29542438-29639896:+|ENST00000565493.1_1_20:34638316-34638640:- OVCH1-AS1 Antisense lncRNA GN=OVCH1-AS1 Antisense E_20221201_NO30_400nL_HLAc1_2e7_directIP_titration_rep4_Slot2-4_1_3519 40mio-rep2 E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep2_Slot2-5_1_3520.10533.1694.3 3 4 1 11.73 3.69 7.32 8.04 80.5 6 1 1 19700.0 Hydrogen 87 (Q) 9 IRFPGDHSV (A) 9 LRFPSSSFS I/R\F|P/G D H\S\V 66.7 6 4 3 1 0 unbiased 36.387684 0.690361 343.1792 1027.532 -0.0095 -9.3 -4.4 6.74 15609.4 nuORFdb_human ENST00000551108.1_1_12:29542438-29639896:+ ENST00000551108.1_1_12:29542438-29639896:+|ENST00000565493.1_1_20:34638316-34638640:- OVCH1-AS1 Antisense lncRNA GN=OVCH1-AS1 Antisense E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep2_Slot2-5_1_3520 40mio-rep3 E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep3_Slot2-5_1_3521.10538.1705.3 3 2 1 11.29 5.21 6.08 6.08 79.3 7 1 1 12800.0 Hydrogen 87 (Q) 9 IRFPGDHSV (A) 9 IRFRSYGE I/R|F|P/G D\H\S\V 77.8 7 5 4 2 0 unbiased 36.407513 0.683446 343.1815 1027.532 -0.0035 -3.4 1.1999999999999997 6.74 15609.4 nuORFdb_human ENST00000551108.1_1_12:29542438-29639896:+ ENST00000551108.1_1_12:29542438-29639896:+|ENST00000565493.1_1_20:34638316-34638640:- OVCH1-AS1 Antisense lncRNA GN=OVCH1-AS1 Antisense E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep3_Slot2-5_1_3521 40mio-rep4 E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep4_Slot2-5_1_3522.10549.1701.3 3 2 1 11.2 3.68 7.52 7.52 80.5 8 1 1 10400.0 Hydrogen 87 (Q) 9 IRFPGDHSV (A) 9 IRFRSYGE I/R\F|P/G/D-H\S\V 100.0 9 4 4 1 1 unbiased 36.431355 0.685931 343.1802 1027.532 -0.0065 -6.3 -1.5999999999999996 6.74 15609.4 nuORFdb_human ENST00000551108.1_1_12:29542438-29639896:+ ENST00000551108.1_1_12:29542438-29639896:+|ENST00000565493.1_1_20:34638316-34638640:- OVCH1-AS1 Antisense lncRNA GN=OVCH1-AS1 Antisense E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep4_Slot2-5_1_3522 40mio-rep1 E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep1_Slot1-04_1_3496.24751.1374.2 2 1 1 14.16 12.12 2.1 2.04 90.5 7 1 1 3170.0 Hydrogen 94 (K) 9 ISARQLIIW (M) 9 LASLQRLLW I/S/A R\Q\L\I|I\W 77.8 7 5 3 1 0 unbiased 85.505608 0.892812 550.3334 1099.662 -0.0035 -3.2 0.0 9.75 12896.8 nuORFdb_human ENST00000573542.5_2_17:7211042-7214672:+ ENST00000573542.5_2_17:7211042-7214672:+|ENST00000572815.5_1_17:7211042-7214672:+|ENST00000573714.5_2_17:7211447-7214672:+ EIF5A 5' Overlap uORF 5' Overlap uORF GN=EIF5A 5' Overlap uORF E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep1_Slot1-04_1_3496 10mio-rep1 E_20221201_NO30_400nL_HLAc1_1e7_directIP_titration_rep1_Slot2-3_1_3512.7794.1439.2 2 1 1 17.8 9.04 6.31 8.76 90.1 8 1 1 3790.0 Hydrogen 61 (T) 10 ISATSSSRNW (G) 10 ISATCKDEKG I/S/A|T|S|S/S/R N\W 80.0 8 4 7 3 0 unbiased 26.932039 0.852002 554.7695 1108.538 -0.0054 -4.9 2.8 9.75 8642.7 nuORFdb_human ENST00000409638.7_1_2:220110217-220111398:+ ENST00000409638.7_1_2:220110217-220111398:+|ENST00000491697.1_1_2:220110217-220110827:+|ENST00000409516.7_1_2:220110217-220112141:+|ENST00000475696.5_1_2:220110350-220111398:+|ENST00000496443.5_1_2:220110461-220110827:+|ENST00000409260.5_1_2:220110626-220111251:+|ENST00000486813.1_1_2:220110626-220111851:+ STK16 5' Overlap uORF 5' Overlap uORF GN=STK16 5' Overlap uORF E_20221201_NO30_400nL_HLAc1_1e7_directIP_titration_rep1_Slot2-3_1_3512 10mio-rep2 E_20221201_NO30_400nL_HLAc1_1e7_directIP_titration_rep2_Slot2-3_1_3513.7744.1443.2 2 2 1 11.62 8.43 2.3 3.19 77.9 8 1 1 7560.0 Hydrogen 61 (T) 10 ISATSSSRNW (G) 10 ISTASWmPSL I/S/A/T/S|S/S R\N\W 80.0 8 3 6 1 0 y-ion bias 26.758116 0.849366 554.7706 1108.538 -0.0034 -3.1 3.6 9.75 8642.7 nuORFdb_human ENST00000409638.7_1_2:220110217-220111398:+ ENST00000409638.7_1_2:220110217-220111398:+|ENST00000491697.1_1_2:220110217-220110827:+|ENST00000409516.7_1_2:220110217-220112141:+|ENST00000475696.5_1_2:220110350-220111398:+|ENST00000496443.5_1_2:220110461-220110827:+|ENST00000409260.5_1_2:220110626-220111251:+|ENST00000486813.1_1_2:220110626-220111851:+ STK16 5' Overlap uORF 5' Overlap uORF GN=STK16 5' Overlap uORF E_20221201_NO30_400nL_HLAc1_1e7_directIP_titration_rep2_Slot2-3_1_3513 10mio-rep3 E_20221201_NO30_400nL_HLAc1_1e7_directIP_titration_rep3_Slot2-3_1_3514.7755.1437.2 2 2 1 13.91 7.52 4.21 6.4 80.9 8 1 1 6980.0 Hydrogen 61 (T) 10 ISATSSSRNW (G) 10 IGTTSnTTAEL I/S/A|T|S|S/S R\N\W 80.0 8 5 6 3 0 unbiased 26.796007 0.852889 554.7719 1108.538 -0.0014 -1.3 5.0 9.75 8642.7 nuORFdb_human ENST00000409638.7_1_2:220110217-220111398:+ ENST00000409638.7_1_2:220110217-220111398:+|ENST00000491697.1_1_2:220110217-220110827:+|ENST00000409516.7_1_2:220110217-220112141:+|ENST00000475696.5_1_2:220110350-220111398:+|ENST00000496443.5_1_2:220110461-220110827:+|ENST00000409260.5_1_2:220110626-220111251:+|ENST00000486813.1_1_2:220110626-220111851:+ STK16 5' Overlap uORF 5' Overlap uORF GN=STK16 5' Overlap uORF E_20221201_NO30_400nL_HLAc1_1e7_directIP_titration_rep3_Slot2-3_1_3514 20mio-rep1 E_20221201_NO30_400nL_HLAc1_2e7_directIP_titration_rep1_Slot2-4_1_3515.7681.1438.2 2 1 1 14.75 7.57 6.57 7.18 81.9 8 1 1 13200.0 Hydrogen 61 (T) 10 ISATSSSRNW (G) 10 ISATCKDEKG I/S/A|T|S/S/S R\N\W 80.0 8 4 6 2 0 unbiased 26.535322 0.852413 554.7682 1108.538 -0.0094 -8.5 -2.3 9.75 8642.7 nuORFdb_human ENST00000409638.7_1_2:220110217-220111398:+ ENST00000409638.7_1_2:220110217-220111398:+|ENST00000491697.1_1_2:220110217-220110827:+|ENST00000409516.7_1_2:220110217-220112141:+|ENST00000475696.5_1_2:220110350-220111398:+|ENST00000496443.5_1_2:220110461-220110827:+|ENST00000409260.5_1_2:220110626-220111251:+|ENST00000486813.1_1_2:220110626-220111851:+ STK16 5' Overlap uORF 5' Overlap uORF GN=STK16 5' Overlap uORF E_20221201_NO30_400nL_HLAc1_2e7_directIP_titration_rep1_Slot2-4_1_3515 20mio-rep2 E_20221201_NO30_400nL_HLAc1_2e7_directIP_titration_rep3_Slot2-4_1_3518.7835.1435.2 2 1 1 11.08 5.65 5.54 5.43 70.3 8 1 1 10400.0 Hydrogen 61 (T) 10 ISATSSSRNW (G) 10 ISTASWmPSL I/S/A/T|S|S/S R\N\W 80.0 8 4 6 2 0 unbiased 27.06768 0.8541 554.7701 1108.538 -0.0054 -4.9 1.3999999999999995 9.75 8642.7 nuORFdb_human ENST00000409638.7_1_2:220110217-220111398:+ ENST00000409638.7_1_2:220110217-220111398:+|ENST00000491697.1_1_2:220110217-220110827:+|ENST00000409516.7_1_2:220110217-220112141:+|ENST00000475696.5_1_2:220110350-220111398:+|ENST00000496443.5_1_2:220110461-220110827:+|ENST00000409260.5_1_2:220110626-220111251:+|ENST00000486813.1_1_2:220110626-220111851:+ STK16 5' Overlap uORF 5' Overlap uORF GN=STK16 5' Overlap uORF E_20221201_NO30_400nL_HLAc1_2e7_directIP_titration_rep3_Slot2-4_1_3518 20mio-rep3 E_20221201_NO30_400nL_HLAc1_2e7_directIP_titration_rep4_Slot2-4_1_3519.7725.1458.2 2 2 1 14.97 7.01 7.53 7.96 83.9 9 1 1 19400.0 Hydrogen 61 (T) 10 ISATSSSRNW (G) 10 ISTASWmPSL I/S/A/T|S/S/S/R\N\W 100.0 10 3 7 1 0 y-ion bias 26.686153 0.839705 554.7692 1108.538 -0.0074 -6.7 -0.9000000000000004 9.75 8642.7 nuORFdb_human ENST00000409638.7_1_2:220110217-220111398:+ ENST00000409638.7_1_2:220110217-220111398:+|ENST00000491697.1_1_2:220110217-220110827:+|ENST00000409516.7_1_2:220110217-220112141:+|ENST00000475696.5_1_2:220110350-220111398:+|ENST00000496443.5_1_2:220110461-220110827:+|ENST00000409260.5_1_2:220110626-220111251:+|ENST00000486813.1_1_2:220110626-220111851:+ STK16 5' Overlap uORF 5' Overlap uORF GN=STK16 5' Overlap uORF E_20221201_NO30_400nL_HLAc1_2e7_directIP_titration_rep4_Slot2-4_1_3519 40mio-rep1 E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep1_Slot1-04_1_3496.7966.1407.2 2 3 1 19.02 10.79 8.14 8.23 92.9 9 1 1 79400.0 Hydrogen 61 (T) 10 ISATSSSRNW (G) 10 ISTASWmPSL I/S/A/T|S|S/S/R\N\W 100.0 10 4 7 2 0 unbiased 27.523443 0.871794 554.7716 1108.538 -0.0014 -1.3 1.9000000000000001 9.75 8642.7 nuORFdb_human ENST00000409638.7_1_2:220110217-220111398:+ ENST00000409638.7_1_2:220110217-220111398:+|ENST00000491697.1_1_2:220110217-220110827:+|ENST00000409516.7_1_2:220110217-220112141:+|ENST00000475696.5_1_2:220110350-220111398:+|ENST00000496443.5_1_2:220110461-220110827:+|ENST00000409260.5_1_2:220110626-220111251:+|ENST00000486813.1_1_2:220110626-220111851:+ STK16 5' Overlap uORF 5' Overlap uORF GN=STK16 5' Overlap uORF E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep1_Slot1-04_1_3496 40mio-rep2 E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep2_Slot2-5_1_3520.7691.1427.2 2 1 1 20.67 10.55 8.62 10.12 95.1 9 1 1 37100.0 Hydrogen 61 (T) 10 ISATSSSRNW (G) 10 IGTTSnTTAEL I/S/A|T|S|S/S/R|N\W 100.0 10 5 8 4 0 dual b/y bias 26.573841 0.8594 554.7693 1108.538 -0.0074 -6.7 -1.7999999999999998 9.75 8642.7 nuORFdb_human ENST00000409638.7_1_2:220110217-220111398:+ ENST00000409638.7_1_2:220110217-220111398:+|ENST00000491697.1_1_2:220110217-220110827:+|ENST00000409516.7_1_2:220110217-220112141:+|ENST00000475696.5_1_2:220110350-220111398:+|ENST00000496443.5_1_2:220110461-220110827:+|ENST00000409260.5_1_2:220110626-220111251:+|ENST00000486813.1_1_2:220110626-220111851:+ STK16 5' Overlap uORF 5' Overlap uORF GN=STK16 5' Overlap uORF E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep2_Slot2-5_1_3520 40mio-rep3 E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep3_Slot2-5_1_3521.7702.1428.2 2 2 1 19.13 11.89 8.62 7.24 93.9 9 1 1 49500.0 Hydrogen 61 (T) 10 ISATSSSRNW (G) 10 ISTASWmPSL I/S/A/T|S|S/S/R|N\W 100.0 10 4 8 3 0 y-ion bias 26.608301 0.858819 554.7703 1108.538 -0.0054 -4.9 -0.3000000000000007 9.75 8642.7 nuORFdb_human ENST00000409638.7_1_2:220110217-220111398:+ ENST00000409638.7_1_2:220110217-220111398:+|ENST00000491697.1_1_2:220110217-220110827:+|ENST00000409516.7_1_2:220110217-220112141:+|ENST00000475696.5_1_2:220110350-220111398:+|ENST00000496443.5_1_2:220110461-220110827:+|ENST00000409260.5_1_2:220110626-220111251:+|ENST00000486813.1_1_2:220110626-220111851:+ STK16 5' Overlap uORF 5' Overlap uORF GN=STK16 5' Overlap uORF E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep3_Slot2-5_1_3521 40mio-rep4 E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep4_Slot2-5_1_3522.7702.1427.2 2 2 1 16.18 10.28 5.77 5.89 87.9 8 1 1 35000.0 Hydrogen 61 (T) 10 ISATSSSRNW (G) 10 ISTASWmPSL I/S/A|T|S|S/S R\N\W 80.0 8 5 6 3 0 unbiased 26.60396 0.859368 554.7697 1108.538 -0.0054 -4.9 -0.20000000000000018 9.75 8642.7 nuORFdb_human ENST00000409638.7_1_2:220110217-220111398:+ ENST00000409638.7_1_2:220110217-220111398:+|ENST00000491697.1_1_2:220110217-220110827:+|ENST00000409516.7_1_2:220110217-220112141:+|ENST00000475696.5_1_2:220110350-220111398:+|ENST00000496443.5_1_2:220110461-220110827:+|ENST00000409260.5_1_2:220110626-220111251:+|ENST00000486813.1_1_2:220110626-220111851:+ STK16 5' Overlap uORF 5' Overlap uORF GN=STK16 5' Overlap uORF E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep4_Slot2-5_1_3522 5mio-rep1 E_20221201_NO30_400nL_HLAc1_5e6_directIP_titration_rep1_Slot1-37_1_3502.7980.1435.2 2 1 1 9.2 6.83 1.71 2.37 78.3 6 1 1 4520.0 Hydrogen 61 (T) 10 ISATSSSRNW (G) 10 ISTASWmPSL I/S/A/T|S/S S R/N W 50.0 5 1 6 1 0 y-ion bias 27.569317 0.854399 554.7691 1108.538 -0.0074 -6.7 0.2999999999999998 9.75 8642.7 nuORFdb_human ENST00000409638.7_1_2:220110217-220111398:+ ENST00000409638.7_1_2:220110217-220111398:+|ENST00000491697.1_1_2:220110217-220110827:+|ENST00000409516.7_1_2:220110217-220112141:+|ENST00000475696.5_1_2:220110350-220111398:+|ENST00000496443.5_1_2:220110461-220110827:+|ENST00000409260.5_1_2:220110626-220111251:+|ENST00000486813.1_1_2:220110626-220111851:+ STK16 5' Overlap uORF 5' Overlap uORF GN=STK16 5' Overlap uORF E_20221201_NO30_400nL_HLAc1_5e6_directIP_titration_rep1_Slot1-37_1_3502 5mio-rep2 E_20221201_NO30_400nL_HLAc1_5e6_directIP_titration_rep2_Slot2-2_1_3510.7948.1444.2 2 2 1 10.45 6.38 3.53 4.07 73.3 7 1 1 6570.0 Hydrogen 61 (T) 10 ISATSSSRNW (G) 10 ISTASWmPSL I/S/A/T/S|S/S R N\W 70.0 7 2 6 1 0 y-ion bias 27.451105 0.848447 554.768 1108.538 -0.0094 -8.5 0.1999999999999993 9.75 8642.7 nuORFdb_human ENST00000409638.7_1_2:220110217-220111398:+ ENST00000409638.7_1_2:220110217-220111398:+|ENST00000491697.1_1_2:220110217-220110827:+|ENST00000409516.7_1_2:220110217-220112141:+|ENST00000475696.5_1_2:220110350-220111398:+|ENST00000496443.5_1_2:220110461-220110827:+|ENST00000409260.5_1_2:220110626-220111251:+|ENST00000486813.1_1_2:220110626-220111851:+ STK16 5' Overlap uORF 5' Overlap uORF GN=STK16 5' Overlap uORF E_20221201_NO30_400nL_HLAc1_5e6_directIP_titration_rep2_Slot2-2_1_3510 1mio-rep1 E_20221201_NO30_400nL_HLAc1_1e6_directIP_titration_rep1_Slot1-06_1_3498.12560.589.1 1 2 1 16.3 6.73 8.54 9.58 94.5 7 0 0 25700.0 Hydrogen 86 (I) 9 ISDPGVQGY (S) 9 LPAGETQGY I/S/D/P G|V|Q\G\Y 77.8 7 4 5 2 0 unbiased 43.385353 1.373025 935.4451 935.447 -0.0019 -2.0 2.9000000000000004 3.8 11588.8 nuORFdb_human ENST00000374647.9_1_9:111693343-111696326:- ENST00000374647.9_1_9:111693343-111696326:- IKBKAP 5' Overlap uORF 5' Overlap uORF GN=IKBKAP 5' Overlap uORF E_20221201_NO30_400nL_HLAc1_1e6_directIP_titration_rep1_Slot1-06_1_3498 1mio-rep2 E_20221201_NO30_400nL_HLAc1_1e6_directIP_titration_rep3_Slot2-1_1_3508.12519.606.1 1 2 1 12.16 5.93 6.15 6.23 94.6 7 0 0 10500.0 Hydrogen 86 (I) 9 ISDPGVQGY (S) 9 LPAGETQGY I/S/D/P G\V|Q\G\Y 77.8 7 4 4 1 0 unbiased 43.239895 1.362987 935.4373 935.447 -0.0099 -10.6 -2.299999999999999 3.8 11588.8 nuORFdb_human ENST00000374647.9_1_9:111693343-111696326:- ENST00000374647.9_1_9:111693343-111696326:- IKBKAP 5' Overlap uORF 5' Overlap uORF GN=IKBKAP 5' Overlap uORF E_20221201_NO30_400nL_HLAc1_1e6_directIP_titration_rep3_Slot2-1_1_3508 1mio-rep3 E_20221201_NO30_400nL_HLAc1_1e6_directIP_titration_rep4_Slot2-1_1_3509.12444.608.1 1 1 1 11.0 4.2 5.09 6.8 90.4 6 0 0 8470.0 Hydrogen 86 (I) 9 ISDPGVQGY (S) 9 LPAGETQGY I S/D/P G|V|Q\G\Y 55.6 5 4 4 2 0 unbiased 42.978767 1.361206 935.4397 935.447 -0.0069 -7.4 0.5999999999999996 3.8 11588.8 nuORFdb_human ENST00000374647.9_1_9:111693343-111696326:- ENST00000374647.9_1_9:111693343-111696326:- IKBKAP 5' Overlap uORF 5' Overlap uORF GN=IKBKAP 5' Overlap uORF E_20221201_NO30_400nL_HLAc1_1e6_directIP_titration_rep4_Slot2-1_1_3509 10mio-rep1 E_20221201_NO30_400nL_HLAc1_1e7_directIP_titration_rep1_Slot2-3_1_3512.12253.603.1 1 5 1 14.24 6.01 7.68 8.24 94.0 8 0 0 110000.0 Hydrogen 86 (I) 9 ISDPGVQGY (S) 9 LPAGETQGY I/S/D/P/G|V|Q\G\Y 100.0 9 4 6 2 0 unbiased 42.332012 1.364769 935.4387 935.447 -0.0079 -8.4 -0.7000000000000002 3.8 11588.8 nuORFdb_human ENST00000374647.9_1_9:111693343-111696326:- ENST00000374647.9_1_9:111693343-111696326:- IKBKAP 5' Overlap uORF 5' Overlap uORF GN=IKBKAP 5' Overlap uORF E_20221201_NO30_400nL_HLAc1_1e7_directIP_titration_rep1_Slot2-3_1_3512 10mio-rep2 E_20221201_NO30_400nL_HLAc1_1e7_directIP_titration_rep2_Slot2-3_1_3513.12234.600.1 1 8 1 15.62 6.29 8.35 9.34 91.6 8 0 0 110000.0 Hydrogen 86 (I) 9 ISDPGVQGY (S) 9 LPAGETQGY I/S/D|P\G|V|Q\G\Y 100.0 9 6 5 3 0 dual b/y bias 42.266674 1.366448 935.4411 935.447 -0.0059 -6.3 0.40000000000000036 3.8 11588.8 nuORFdb_human ENST00000374647.9_1_9:111693343-111696326:- ENST00000374647.9_1_9:111693343-111696326:- IKBKAP 5' Overlap uORF 5' Overlap uORF GN=IKBKAP 5' Overlap uORF E_20221201_NO30_400nL_HLAc1_1e7_directIP_titration_rep2_Slot2-3_1_3513 10mio-rep3 E_20221201_NO30_400nL_HLAc1_1e7_directIP_titration_rep3_Slot2-3_1_3514.12223.600.1 1 8 1 15.36 6.81 7.72 8.55 93.3 7 0 0 243000.0 Hydrogen 86 (I) 9 ISDPGVQGY (S) 9 LPAGETQGY I/S/D/P G|V|Q\G\Y 77.8 7 4 5 2 0 unbiased 42.228924 1.366185 935.4417 935.447 -0.0049 -5.2 1.0999999999999996 3.8 11588.8 nuORFdb_human ENST00000374647.9_1_9:111693343-111696326:- ENST00000374647.9_1_9:111693343-111696326:- IKBKAP 5' Overlap uORF 5' Overlap uORF GN=IKBKAP 5' Overlap uORF E_20221201_NO30_400nL_HLAc1_1e7_directIP_titration_rep3_Slot2-3_1_3514 20mio-rep1 E_20221201_NO30_400nL_HLAc1_2e7_directIP_titration_rep1_Slot2-4_1_3515.12157.600.1 1 5 1 15.18 7.39 8.33 7.8 95.3 7 0 0 442000.0 Hydrogen 86 (I) 9 ISDPGVQGY (S) 9 LPAGETQGY I/S/D/P G|V|Q\G\Y 77.8 7 4 5 2 0 unbiased 41.990925 1.366412 935.4421 935.447 -0.0049 -5.2 1.0 3.8 11588.8 nuORFdb_human ENST00000374647.9_1_9:111693343-111696326:- ENST00000374647.9_1_9:111693343-111696326:- IKBKAP 5' Overlap uORF 5' Overlap uORF GN=IKBKAP 5' Overlap uORF E_20221201_NO30_400nL_HLAc1_2e7_directIP_titration_rep1_Slot2-4_1_3515 20mio-rep2 E_20221201_NO30_400nL_HLAc1_2e7_directIP_titration_rep3_Slot2-4_1_3518.12289.1545.2 2 9 1 15.57 7.89 8.17 7.68 86.7 7 0 0 204000.0 Hydrogen 86 (I) 9 ISDPGVQGY (S) 9 LPAGETQGY I/S/D|P|G V|Q|G\Y 77.8 7 5 6 4 0 dual b/y bias 42.44799 0.785003 468.2239 935.447 -0.0062 -6.6 -0.2999999999999998 3.8 11588.8 nuORFdb_human ENST00000374647.9_1_9:111693343-111696326:- ENST00000374647.9_1_9:111693343-111696326:- IKBKAP 5' Overlap uORF 5' Overlap uORF GN=IKBKAP 5' Overlap uORF E_20221201_NO30_400nL_HLAc1_2e7_directIP_titration_rep3_Slot2-4_1_3518 20mio-rep3 E_20221201_NO30_400nL_HLAc1_2e7_directIP_titration_rep4_Slot2-4_1_3519.12201.586.1 1 6 1 18.38 8.24 9.86 10.14 94.2 8 0 0 331000.0 Hydrogen 86 (I) 9 ISDPGVQGY (S) 9 SnLPGNYGL I/S/D|P|G|V|Q\G\Y 100.0 9 6 6 4 0 dual b/y bias 42.147316 1.374932 935.4442 935.447 -0.0029 -3.1 2.6999999999999997 3.8 11588.8 nuORFdb_human ENST00000374647.9_1_9:111693343-111696326:- ENST00000374647.9_1_9:111693343-111696326:- IKBKAP 5' Overlap uORF 5' Overlap uORF GN=IKBKAP 5' Overlap uORF E_20221201_NO30_400nL_HLAc1_2e7_directIP_titration_rep4_Slot2-4_1_3519 40mio-rep1 E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep1_Slot1-04_1_3496.12443.574.1 1 4 1 14.59 6.29 6.74 8.3 92.8 7 0 0 269000.0 Hydrogen 86 (I) 9 ISDPGVQGY (S) 9 LPAGETQGY I/S/D/P G|V|Q\G\Y 77.8 7 4 5 2 0 unbiased 42.982182 1.381837 935.4459 935.447 -0.0009 -0.9 2.3000000000000003 3.8 11588.8 nuORFdb_human ENST00000374647.9_1_9:111693343-111696326:- ENST00000374647.9_1_9:111693343-111696326:- IKBKAP 5' Overlap uORF 5' Overlap uORF GN=IKBKAP 5' Overlap uORF E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep1_Slot1-04_1_3496 40mio-rep2 E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep2_Slot2-5_1_3520.12157.584.1 1 5 1 16.66 8.1 8.91 8.56 94.8 8 0 0 430000.0 Hydrogen 86 (I) 9 ISDPGVQGY (S) 9 SnLPGNYGL I/S/D/P/G|V|Q\G\Y 100.0 9 4 6 2 0 unbiased 41.997749 1.375843 935.4432 935.447 -0.0039 -4.2 0.7000000000000002 3.8 11588.8 nuORFdb_human ENST00000374647.9_1_9:111693343-111696326:- ENST00000374647.9_1_9:111693343-111696326:- IKBKAP 5' Overlap uORF 5' Overlap uORF GN=IKBKAP 5' Overlap uORF E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep2_Slot2-5_1_3520 40mio-rep3 E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep3_Slot2-5_1_3521.12179.1537.2 2 5 1 17.29 9.65 9.14 7.64 89.2 7 0 0 318000.0 Hydrogen 86 (I) 9 ISDPGVQGY (S) 9 LPAGETQGY I/S/D|P|G V|Q|G\Y 77.8 7 5 6 4 0 dual b/y bias 42.07407 0.790123 468.2255 935.447 -0.0022 -2.3 2.3 3.8 11588.8 nuORFdb_human ENST00000374647.9_1_9:111693343-111696326:- ENST00000374647.9_1_9:111693343-111696326:- IKBKAP 5' Overlap uORF 5' Overlap uORF GN=IKBKAP 5' Overlap uORF E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep3_Slot2-5_1_3521 40mio-rep4 E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep4_Slot2-5_1_3522.12157.1536.2 2 5 1 16.96 8.75 7.84 8.2 89.4 7 0 0 408000.0 Hydrogen 86 (I) 9 ISDPGVQGY (S) 9 LPAGETQGY I/S/D|P|G V|Q|G\Y 77.8 7 5 6 4 0 dual b/y bias 41.983147 0.791093 468.2243 935.447 -0.0062 -6.6 -1.8999999999999995 3.8 11588.8 nuORFdb_human ENST00000374647.9_1_9:111693343-111696326:- ENST00000374647.9_1_9:111693343-111696326:- IKBKAP 5' Overlap uORF 5' Overlap uORF GN=IKBKAP 5' Overlap uORF E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep4_Slot2-5_1_3522 5mio-rep1 E_20221201_NO30_400nL_HLAc1_5e6_directIP_titration_rep1_Slot1-37_1_3502.12498.588.1 1 3 1 15.61 6.2 9.3 9.4 93.5 8 0 0 126000.0 Hydrogen 86 (I) 9 ISDPGVQGY (S) 9 LPAGETQGY I/S/D/P/G|V|Q\G\Y 100.0 9 4 6 2 0 unbiased 43.172268 1.373484 935.4409 935.447 -0.0059 -6.3 0.7000000000000002 3.8 11588.8 nuORFdb_human ENST00000374647.9_1_9:111693343-111696326:- ENST00000374647.9_1_9:111693343-111696326:- IKBKAP 5' Overlap uORF 5' Overlap uORF GN=IKBKAP 5' Overlap uORF E_20221201_NO30_400nL_HLAc1_5e6_directIP_titration_rep1_Slot1-37_1_3502 5mio-rep2 E_20221201_NO30_400nL_HLAc1_5e6_directIP_titration_rep2_Slot2-2_1_3510.12432.606.1 1 2 1 12.31 4.4 6.8 7.91 88.8 6 0 0 37900.0 Hydrogen 86 (I) 9 ISDPGVQGY (S) 9 LPAGETQGY I S/D/P G|V|Q\G\Y 55.6 5 4 4 2 0 unbiased 42.939892 1.362543 935.4398 935.447 -0.0069 -7.4 1.299999999999999 3.8 11588.8 nuORFdb_human ENST00000374647.9_1_9:111693343-111696326:- ENST00000374647.9_1_9:111693343-111696326:- IKBKAP 5' Overlap uORF 5' Overlap uORF GN=IKBKAP 5' Overlap uORF E_20221201_NO30_400nL_HLAc1_5e6_directIP_titration_rep2_Slot2-2_1_3510 5mio-rep3 E_20221201_NO30_400nL_HLAc1_5e6_directIP_titration_rep3_Slot2-2_1_3511.12454.601.1 1 2 1 17.04 6.81 10.06 10.24 91.6 8 0 0 70100.0 Hydrogen 86 (I) 9 ISDPGVQGY (S) 9 LPAGETQGY I/S/D|P|G|V|Q\G\Y 100.0 9 6 6 4 0 dual b/y bias 43.023499 1.365449 935.4395 935.447 -0.0069 -7.4 0.7999999999999989 3.8 11588.8 nuORFdb_human ENST00000374647.9_1_9:111693343-111696326:- ENST00000374647.9_1_9:111693343-111696326:- IKBKAP 5' Overlap uORF 5' Overlap uORF GN=IKBKAP 5' Overlap uORF E_20221201_NO30_400nL_HLAc1_5e6_directIP_titration_rep3_Slot2-2_1_3511 10mio-rep1 E_20221201_NO30_400nL_HLAc1_1e7_directIP_titration_rep1_Slot2-3_1_3512.18241.1483.2 2 1 1 7.74 5.88 -0.31 1.86 74.9 6 0 0 9410.0 Hydrogen 45 (Y) 9 ISIHYGAEW (-) 9 GLAGnGTVAEW I/S/I H|Y-G A\E\W 55.6 5 3 3 1 1 unbiased 63.011909 0.824153 538.2591 1075.521 -0.01 -9.3 -1.6000000000000005 5.24 6384.4 nuORFdb_human ENST00000465271.5_1_1:224548240-224559008:+ ENST00000465271.5_1_1:224548240-224559008:+ CNIH4 Out-of-Frame Out-of-Frame GN=CNIH4 Out-of-Frame E_20221201_NO30_400nL_HLAc1_1e7_directIP_titration_rep1_Slot2-3_1_3512 10mio-rep2 E_20221201_NO30_400nL_HLAc1_1e7_directIP_titration_rep2_Slot2-3_1_3513.18184.1508.2 2 4 1 11.53 6.68 3.5 4.85 82.0 6 0 0 19500.0 Hydrogen 45 (Y) 9 ISIHYGAEW (-) 9 IRGSDNVEW I/S/I H|Y G/A|E\W 55.6 5 3 5 2 0 unbiased 62.811783 0.808433 538.26 1075.521 -0.008 -7.4 -0.7000000000000002 5.24 6384.4 nuORFdb_human ENST00000465271.5_1_1:224548240-224559008:+ ENST00000465271.5_1_1:224548240-224559008:+ CNIH4 Out-of-Frame Out-of-Frame GN=CNIH4 Out-of-Frame E_20221201_NO30_400nL_HLAc1_1e7_directIP_titration_rep2_Slot2-3_1_3513 10mio-rep3 E_20221201_NO30_400nL_HLAc1_1e7_directIP_titration_rep3_Slot2-3_1_3514.18140.1495.2 2 4 1 13.58 8.63 4.84 4.95 83.9 7 0 0 29400.0 Hydrogen 45 (Y) 9 ISIHYGAEW (-) 9 nWKALQEW I/S/I H|Y|G\A|E|W 77.8 7 5 6 4 0 dual b/y bias 62.662441 0.816829 538.2614 1075.521 -0.006 -5.6 0.7000000000000002 5.24 6384.4 nuORFdb_human ENST00000465271.5_1_1:224548240-224559008:+ ENST00000465271.5_1_1:224548240-224559008:+ CNIH4 Out-of-Frame Out-of-Frame GN=CNIH4 Out-of-Frame E_20221201_NO30_400nL_HLAc1_1e7_directIP_titration_rep3_Slot2-3_1_3514 20mio-rep1 E_20221201_NO30_400nL_HLAc1_2e7_directIP_titration_rep1_Slot2-4_1_3515.18187.1451.2 2 3 1 13.43 6.47 5.54 6.96 81.6 7 0 0 24100.0 Hydrogen 45 (Y) 9 ISIHYGAEW (-) 9 IRGSDNVEW I/S/I H|Y\G|A|E\W 77.8 7 5 5 3 0 dual b/y bias 62.810326 0.844108 538.2621 1075.521 -0.004 -3.7 2.5 5.24 6384.4 nuORFdb_human ENST00000465271.5_1_1:224548240-224559008:+ ENST00000465271.5_1_1:224548240-224559008:+ CNIH4 Out-of-Frame Out-of-Frame GN=CNIH4 Out-of-Frame E_20221201_NO30_400nL_HLAc1_2e7_directIP_titration_rep1_Slot2-4_1_3515 20mio-rep2 E_20221201_NO30_400nL_HLAc1_2e7_directIP_titration_rep3_Slot2-4_1_3518.18240.1478.2 2 2 1 15.18 8.61 6.57 6.57 88.2 8 0 0 28300.0 Hydrogen 45 (Y) 9 ISIHYGAEW (-) 9 IRGSDNVEW I/S/I/H\Y\G\A|E\W 100.0 9 5 4 1 0 unbiased 63.002762 0.827119 538.2604 1075.521 -0.008 -7.4 -1.1000000000000005 5.24 6384.4 nuORFdb_human ENST00000465271.5_1_1:224548240-224559008:+ ENST00000465271.5_1_1:224548240-224559008:+ CNIH4 Out-of-Frame Out-of-Frame GN=CNIH4 Out-of-Frame E_20221201_NO30_400nL_HLAc1_2e7_directIP_titration_rep3_Slot2-4_1_3518 20mio-rep3 E_20221201_NO30_400nL_HLAc1_2e7_directIP_titration_rep4_Slot2-4_1_3519.18208.1482.2 2 4 1 13.04 5.76 6.38 7.28 84.0 8 0 0 32900.0 Hydrogen 45 (Y) 9 ISIHYGAEW (-) 9 IGVGVGAMGEW I/S/I/H|Y\G\A|E\W 100.0 9 5 5 2 0 unbiased 62.887695 0.825166 538.2601 1075.521 -0.008 -7.4 -1.6000000000000005 5.24 6384.4 nuORFdb_human ENST00000465271.5_1_1:224548240-224559008:+ ENST00000465271.5_1_1:224548240-224559008:+ CNIH4 Out-of-Frame Out-of-Frame GN=CNIH4 Out-of-Frame E_20221201_NO30_400nL_HLAc1_2e7_directIP_titration_rep4_Slot2-4_1_3519 40mio-rep1 E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep1_Slot1-04_1_3496.18394.1457.2 2 9 1 17.3 7.18 8.32 10.12 93.6 8 0 0 155000.0 Hydrogen 45 (Y) 9 ISIHYGAEW (-) 9 nWKALQEW I/S/I/H|Y|G|A|E\W 100.0 9 5 7 4 0 dual b/y bias 63.539322 0.840283 538.2616 1075.521 -0.004 -3.7 -0.5 5.24 6384.4 nuORFdb_human ENST00000465271.5_1_1:224548240-224559008:+ ENST00000465271.5_1_1:224548240-224559008:+ CNIH4 Out-of-Frame Out-of-Frame GN=CNIH4 Out-of-Frame E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep1_Slot1-04_1_3496 40mio-rep2 E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep2_Slot2-5_1_3520.18186.1463.2 2 5 1 15.22 8.11 5.56 7.11 90.9 7 0 0 91300.0 Hydrogen 45 (Y) 9 ISIHYGAEW (-) 9 IGVGVGAMGEW I/S/I H|Y\G\A|E\W 77.8 7 5 4 2 0 unbiased 62.822079 0.836753 538.2611 1075.521 -0.006 -5.6 -0.6999999999999993 5.24 6384.4 nuORFdb_human ENST00000465271.5_1_1:224548240-224559008:+ ENST00000465271.5_1_1:224548240-224559008:+ CNIH4 Out-of-Frame Out-of-Frame GN=CNIH4 Out-of-Frame E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep2_Slot2-5_1_3520 40mio-rep3 E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep3_Slot2-5_1_3521.18155.1469.2 2 4 1 15.71 7.48 5.57 8.23 89.9 8 0 0 68400.0 Hydrogen 45 (Y) 9 ISIHYGAEW (-) 9 qILEFQEW I/S/I/H|Y|G|A|E\W 100.0 9 5 7 4 0 dual b/y bias 62.716064 0.833054 538.2612 1075.521 -0.006 -5.6 -1.0 5.24 6384.4 nuORFdb_human ENST00000465271.5_1_1:224548240-224559008:+ ENST00000465271.5_1_1:224548240-224559008:+ CNIH4 Out-of-Frame Out-of-Frame GN=CNIH4 Out-of-Frame E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep3_Slot2-5_1_3521 40mio-rep4 E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep4_Slot2-5_1_3522.18242.1453.2 2 3 1 15.7 8.05 6.11 7.64 91.0 8 0 0 56800.0 Hydrogen 45 (Y) 9 ISIHYGAEW (-) 9 IRGSDNVEW I/S/I/H|Y\G\A|E\W 100.0 9 5 5 2 0 unbiased 62.998768 0.842844 538.2618 1075.521 -0.004 -3.7 1.0 5.24 6384.4 nuORFdb_human ENST00000465271.5_1_1:224548240-224559008:+ ENST00000465271.5_1_1:224548240-224559008:+ CNIH4 Out-of-Frame Out-of-Frame GN=CNIH4 Out-of-Frame E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep4_Slot2-5_1_3522 5mio-rep1 E_20221201_NO30_400nL_HLAc1_5e6_directIP_titration_rep1_Slot1-37_1_3502.18479.1480.2 2 7 1 12.99 6.98 5.5 6.02 83.3 8 0 0 34800.0 Hydrogen 45 (Y) 9 ISIHYGAEW (-) 9 IGVGVGAMGEW I/S/I/H/Y\G\A|E\W 100.0 9 4 5 1 0 unbiased 63.827034 0.82604 538.2598 1075.521 -0.008 -7.4 -0.40000000000000036 5.24 6384.4 nuORFdb_human ENST00000465271.5_1_1:224548240-224559008:+ ENST00000465271.5_1_1:224548240-224559008:+ CNIH4 Out-of-Frame Out-of-Frame GN=CNIH4 Out-of-Frame E_20221201_NO30_400nL_HLAc1_5e6_directIP_titration_rep1_Slot1-37_1_3502 20mio-rep1 E_20221201_NO30_400nL_HLAc1_2e7_directIP_titration_rep1_Slot2-4_1_3515.14386.1476.2 2 1 1 8.06 5.47 2.19 2.59 65.5 6 0 0 6270.0 Hydrogen 11 (S) 9 ISLHSTASW (A) 9 VNNLPTASW I/S L H\S\T|A|S\W 66.7 6 5 3 2 0 unbiased 49.68668 0.828427 501.2535 1001.505 -0.0043 -4.3 1.9000000000000004 6.74 4538.1 nuORFdb_human ENST00000539156.5_1_15:80450477-80452179:+ ENST00000539156.5_1_15:80450477-80452179:+ FAH 5' Overlap uORF 5' Overlap uORF GN=FAH 5' Overlap uORF E_20221201_NO30_400nL_HLAc1_2e7_directIP_titration_rep1_Slot2-4_1_3515 40mio-rep1 E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep1_Slot1-04_1_3496.14646.1452.2 2 1 1 12.82 8.08 3.3 4.74 85.3 7 0 0 19100.0 Hydrogen 11 (S) 9 ISLHSTASW (A) 9 VNNLPTASW I/S/L H|S\T\A|S\W 77.8 7 5 4 2 0 unbiased 50.59161 0.843549 501.2554 1001.505 -0.0023 -2.3 0.9000000000000004 6.74 4538.1 nuORFdb_human ENST00000539156.5_1_15:80450477-80452179:+ ENST00000539156.5_1_15:80450477-80452179:+ FAH 5' Overlap uORF 5' Overlap uORF GN=FAH 5' Overlap uORF E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep1_Slot1-04_1_3496 40mio-rep2 E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep2_Slot2-5_1_3520.14347.1463.2 2 1 1 13.07 7.98 4.38 5.08 86.9 7 0 0 16400.0 Hydrogen 11 (S) 9 ISLHSTASW (A) 9 LSSVHSVSW I/S/L H|S\T\A|S\W 77.8 7 5 4 2 0 unbiased 49.560329 0.836589 501.2535 1001.505 -0.0043 -4.3 0.6000000000000005 6.74 4538.1 nuORFdb_human ENST00000539156.5_1_15:80450477-80452179:+ ENST00000539156.5_1_15:80450477-80452179:+ FAH 5' Overlap uORF 5' Overlap uORF GN=FAH 5' Overlap uORF E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep2_Slot2-5_1_3520 40mio-rep3 E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep3_Slot2-5_1_3521.14356.1466.2 2 2 1 11.04 6.07 3.23 4.97 84.7 6 0 0 26000.0 Hydrogen 11 (S) 9 ISLHSTASW (A) 9 LQSNGLPSW I/S L H|S\T\A|S\W 66.7 6 5 3 2 0 unbiased 49.594589 0.834667 501.254 1001.505 -0.0043 -4.3 0.2999999999999998 6.74 4538.1 nuORFdb_human ENST00000539156.5_1_15:80450477-80452179:+ ENST00000539156.5_1_15:80450477-80452179:+ FAH 5' Overlap uORF 5' Overlap uORF GN=FAH 5' Overlap uORF E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep3_Slot2-5_1_3521 40mio-rep4 E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep4_Slot2-5_1_3522.14351.1467.2 2 1 1 11.77 9.39 2.79 2.37 83.5 6 0 0 10500.0 Hydrogen 11 (S) 9 ISLHSTASW (A) 9 VNNLPTASW I/S L H\S\T\A|S\W 66.7 6 5 2 1 0 b-ion bias 49.563271 0.834572 501.2535 1001.505 -0.0043 -4.3 0.40000000000000036 6.74 4538.1 nuORFdb_human ENST00000539156.5_1_15:80450477-80452179:+ ENST00000539156.5_1_15:80450477-80452179:+ FAH 5' Overlap uORF 5' Overlap uORF GN=FAH 5' Overlap uORF E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep4_Slot2-5_1_3522 SCP E_20221201_NO30_400nL_HLAc1_4e7_PDAC_Slot2-6_1_3524.28252.1471.2 2 1 1 8.19 5.69 2.05 2.5 66.8 7 0 1 22800.0 Hydrogen 41 (T) 8 ISLWDVRP (S) 8 GAAGASRPVSL I/S/L/W-D-V/R\P 100.0 8 1 4 0 2 y-ion bias 97.605614 0.831696 493.2742 985.547 -0.0058 -5.9 -3.5000000000000004 5.84 10241.1 nuORFdb_human ENST00000368895.2_1_10:124648046-124657367:+ ENST00000368895.2_1_10:124648046-124657367:+ RP11-564D11.3 Pseudogene Pseudogene GN=RP11-564D11.3 Pseudogene E_20221201_NO30_400nL_HLAc1_4e7_PDAC_Slot2-6_1_3524 Eva-50mio-3fr AL20210827_EV_HLA_PDAC_PANFRO413_2D_50e6_W632_classI_fxn1.32470.32470.2 2 1 1 7.5 5.93 -0.78 1.57 50.3 6 0 0 3680000.0 Hydrogen 54 (A) 10 ISSHWFLPQI (H) 10 HPLCALYIQL I/S\S/H/W F L P|Q\I 60.0 6 3 4 1 0 unbiased 0.994 90.9 58.37 100.0 0.0 614.328 1227.652 -0.0033 -2.7 -2.8000000000000003 6.74 14789.7 smORF_v4 LINC00303_1:204010221-204006715:- LINC00303_1:204010221-204006715:- LINC00303 lncRNA 1:204010221-204006715:- 1 lincRNA GN=LINC00303 AL20210827_EV_HLA_PDAC_PANFRO413_2D_50e6_W632_classI_fxn1 20mio-rep3 E_20221201_NO30_400nL_HLAc1_2e7_directIP_titration_rep4_Slot2-4_1_3519.18767.574.1 1 1 1 9.59 6.68 3.07 2.91 70.4 5 0 0 3240.0 M40m Hydrogen 37 (E) 8 ISSmASLW (L) 8 SSLmTGLW I S S/m\A\S\L\W 62.5 5 4 1 0 0 b-ion bias m:Oxidized methionine 64.817879 1.38156 910.4285 894.439 15.989 -6.5 -0.7000000000000002 5.52 9304.9 nuORFdb_human ENST00000465860.5_1_9:37782001-37784011:- ENST00000465860.5_1_9:37782001-37784011:- EXOSC3 ncRNA Retained Intron lncRNA GN=EXOSC3 ncRNA Retained Intron E_20221201_NO30_400nL_HLAc1_2e7_directIP_titration_rep4_Slot2-4_1_3519 40mio-rep2 E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep2_Slot2-5_1_3520.18683.570.1 1 2 1 10.9 7.77 3.79 3.12 79.7 5 0 0 11300.0 M40m Hydrogen 37 (E) 8 ISSmASLW (L) 8 ALSmSSLW I S/S m\A\S\L\W 50.0 4 4 1 0 0 b-ion bias m:Oxidized methionine 64.540749 1.384224 910.4296 894.439 15.991 -4.3 0.6000000000000005 5.52 9304.9 nuORFdb_human ENST00000465860.5_1_9:37782001-37784011:- ENST00000465860.5_1_9:37782001-37784011:- EXOSC3 ncRNA Retained Intron lncRNA GN=EXOSC3 ncRNA Retained Intron E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep2_Slot2-5_1_3520 40mio-rep4 E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep4_Slot2-5_1_3522.18690.573.1 1 2 1 8.59 5.44 3.38 3.15 69.2 5 0 0 12400.0 M40m Hydrogen 37 (E) 8 ISSmASLW (L) 8 SSLmTGLW I S/S m\A\S\L\W 50.0 4 4 1 0 0 b-ion bias m:Oxidized methionine 64.546379 1.382607 910.4274 894.439 15.988 -7.6 -2.8999999999999995 5.52 9304.9 nuORFdb_human ENST00000465860.5_1_9:37782001-37784011:- ENST00000465860.5_1_9:37782001-37784011:- EXOSC3 ncRNA Retained Intron lncRNA GN=EXOSC3 ncRNA Retained Intron E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep4_Slot2-5_1_3522 40mio-rep3 E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep3_Slot2-5_1_3521.6835.1420.2 2 1 1 7.1 2.75 5.73 4.35 55.2 8 1 1 9140.0 Hydrogen 67 (I) 11 ISSSSTGKTSW (T) 11 LSSSDmLATSL I/S/S/S/S/T/G K T\S\W 72.7 8 2 6 0 0 y-ion bias 23.614265 0.864033 570.7769 1140.553 -0.0064 -5.6 -1.0 8.75 9358.7 nuORFdb_human ENST00000395879.5_2_7:43680244-43687232:- ENST00000395879.5_2_7:43680244-43687232:- COA1 Out-of-Frame Out-of-Frame GN=COA1 Out-of-Frame E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep3_Slot2-5_1_3521 20mio-rep1 E_20221201_NO30_400nL_HLAc1_2e7_directIP_titration_rep1_Slot2-4_1_3515.23151.569.1 1 1 1 14.13 7.59 7.74 6.54 86.4 5 0 0 4880.0 Hydrogen 39 (V) 9 ITAGLPATW (H) 9 SAALLTPAW I T/A G|L|P A|T\W 33.3 3 4 4 3 0 dual b/y bias 79.947411 1.384633 929.5045 929.509 -0.0041 -4.4 1.7999999999999998 5.52 7248.1 nuORFdb_human ENST00000391919.1_1_19:46857064-46878873:+ ENST00000391919.1_1_19:46857064-46878873:+ PPP5C 5' Overlap uORF 5' Overlap uORF GN=PPP5C 5' Overlap uORF E_20221201_NO30_400nL_HLAc1_2e7_directIP_titration_rep1_Slot2-4_1_3515 20mio-rep2 E_20221201_NO30_400nL_HLAc1_2e7_directIP_titration_rep3_Slot2-4_1_3518.23200.565.1 1 2 1 10.64 6.21 6.03 4.43 82.1 5 0 0 6080.0 Hydrogen 39 (V) 9 ITAGLPATW (H) 9 SAALLTPAW I T/A G\L|P A|T\W 33.3 3 4 3 2 0 unbiased 80.133461 1.38723 929.505 929.509 -0.0041 -4.4 1.8999999999999995 5.52 7248.1 nuORFdb_human ENST00000391919.1_1_19:46857064-46878873:+ ENST00000391919.1_1_19:46857064-46878873:+ PPP5C 5' Overlap uORF 5' Overlap uORF GN=PPP5C 5' Overlap uORF E_20221201_NO30_400nL_HLAc1_2e7_directIP_titration_rep3_Slot2-4_1_3518 20mio-rep3 E_20221201_NO30_400nL_HLAc1_2e7_directIP_titration_rep4_Slot2-4_1_3519.23200.553.1 1 2 1 13.96 9.08 6.65 4.88 89.8 7 0 0 4300.0 Hydrogen 39 (V) 9 ITAGLPATW (H) 9 LWRPATW I T/A/G|L|P\A/T\W 77.8 7 4 5 2 0 unbiased 80.124802 1.394553 929.5042 929.509 -0.0051 -5.5 0.2999999999999998 5.52 7248.1 nuORFdb_human ENST00000391919.1_1_19:46857064-46878873:+ ENST00000391919.1_1_19:46857064-46878873:+ PPP5C 5' Overlap uORF 5' Overlap uORF GN=PPP5C 5' Overlap uORF E_20221201_NO30_400nL_HLAc1_2e7_directIP_titration_rep4_Slot2-4_1_3519 40mio-rep1 E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep1_Slot1-04_1_3496.23370.537.1 1 1 1 14.2 8.77 7.54 5.43 89.5 6 0 0 3640.0 Hydrogen 39 (V) 9 ITAGLPATW (H) 9 SAALLTPAW I T/A G|L|P\A|T\W 55.6 5 5 4 3 0 dual b/y bias 80.733528 1.403839 929.5047 929.509 -0.0041 -4.4 -1.2000000000000002 5.52 7248.1 nuORFdb_human ENST00000391919.1_1_19:46857064-46878873:+ ENST00000391919.1_1_19:46857064-46878873:+ PPP5C 5' Overlap uORF 5' Overlap uORF GN=PPP5C 5' Overlap uORF E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep1_Slot1-04_1_3496 40mio-rep2 E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep2_Slot2-5_1_3520.23138.559.1 1 1 1 15.57 8.33 8.87 7.24 94.9 7 0 0 5120.0 Hydrogen 39 (V) 9 ITAGLPATW (H) 9 LWRPATW I T/A/G|L|P\A|T\W 77.8 7 5 5 3 0 dual b/y bias 79.930984 1.390545 929.5043 929.509 -0.0051 -5.5 -0.5999999999999996 5.52 7248.1 nuORFdb_human ENST00000391919.1_1_19:46857064-46878873:+ ENST00000391919.1_1_19:46857064-46878873:+ PPP5C 5' Overlap uORF 5' Overlap uORF GN=PPP5C 5' Overlap uORF E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep2_Slot2-5_1_3520 40mio-rep3 E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep3_Slot2-5_1_3521.23155.544.1 1 2 1 15.33 8.43 8.55 6.89 92.5 7 0 0 8700.0 Hydrogen 39 (V) 9 ITAGLPATW (H) 9 SAALLTPAW I T/A/G|L|P\A|T\W 77.8 7 5 5 3 0 dual b/y bias 79.98703 1.39993 929.5036 929.509 -0.0051 -5.5 -0.9000000000000004 5.52 7248.1 nuORFdb_human ENST00000391919.1_1_19:46857064-46878873:+ ENST00000391919.1_1_19:46857064-46878873:+ PPP5C 5' Overlap uORF 5' Overlap uORF GN=PPP5C 5' Overlap uORF E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep3_Slot2-5_1_3521 40mio-rep4 E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep4_Slot2-5_1_3522.23142.555.1 1 1 1 14.86 8.38 8.25 6.48 90.4 7 0 0 2760.0 Hydrogen 39 (V) 9 ITAGLPATW (H) 9 LWRPATW I T/A/G|L|P\A|T\W 77.8 7 5 5 3 0 dual b/y bias 79.931046 1.393456 929.5083 929.509 -0.0011 -1.2 3.5 5.52 7248.1 nuORFdb_human ENST00000391919.1_1_19:46857064-46878873:+ ENST00000391919.1_1_19:46857064-46878873:+ PPP5C 5' Overlap uORF 5' Overlap uORF GN=PPP5C 5' Overlap uORF E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep4_Slot2-5_1_3522 40mio-rep1 E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep1_Slot1-04_1_3496.7712.1451.2 2 1 1 9.93 7.0 2.63 2.93 72.2 7 1 1 4580.0 Hydrogen 29 (Q) 9 ITEAGQRSW (L) 9 LTEAIKEEn I/T|E/A|G/Q-R S\W 77.8 7 3 5 2 1 unbiased 26.646784 0.844436 524.2627 1047.522 -0.0031 -2.9 0.30000000000000027 6.0 4664.2 nuORFdb_human ENST00000334512.9_1_10:80899522-80961376:+ ENST00000334512.9_1_10:80899522-80961376:+ ZMIZ1 5' uORF 5' uORF GN=ZMIZ1 5' uORF E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep1_Slot1-04_1_3496 10mio-rep1 E_20221201_NO30_400nL_HLAc1_1e7_directIP_titration_rep1_Slot2-3_1_3512.24861.316.1 1 1 1 9.5 4.59 3.8 4.91 75.1 6 0 0 1360.0 Hydrogen 10 (R) 9 ITELVTLTY (L) 9 TLLTLELSY I T E\L|V\T\L\T\Y 66.7 6 6 1 1 0 b-ion bias 85.873535 1.534958 1052.5843 1052.587 -0.0074 -7.0 0.7000000000000002 4.0 2838.3 nuORFdb_human ENST00000633556.1_1_6:106975319-106978099:+ ENST00000633556.1_1_6:106975319-106978099:+ AIM1 Out-of-Frame Out-of-Frame GN=AIM1 Out-of-Frame E_20221201_NO30_400nL_HLAc1_1e7_directIP_titration_rep1_Slot2-3_1_3512 10mio-rep2 E_20221201_NO30_400nL_HLAc1_1e7_directIP_titration_rep2_Slot2-3_1_3513.24784.320.1 1 2 1 9.9 4.16 5.34 5.74 74.9 6 0 0 3190.0 Hydrogen 10 (R) 9 ITELVTLTY (L) 9 TELVTLTYL I T E\L\V\T|L\T\Y 66.7 6 6 1 1 0 b-ion bias 85.60041 1.532291 1052.5806 1052.587 -0.0074 -7.0 -0.2999999999999998 4.0 2838.3 nuORFdb_human ENST00000633556.1_1_6:106975319-106978099:+ ENST00000633556.1_1_6:106975319-106978099:+ AIM1 Out-of-Frame Out-of-Frame GN=AIM1 Out-of-Frame E_20221201_NO30_400nL_HLAc1_1e7_directIP_titration_rep2_Slot2-3_1_3513 20mio-rep1 E_20221201_NO30_400nL_HLAc1_2e7_directIP_titration_rep1_Slot2-4_1_3515.24749.318.1 1 3 1 8.83 4.36 4.37 4.47 73.8 6 0 0 9540.0 Hydrogen 10 (R) 9 ITELVTLTY (L) 9 LDITTLITY I T E\L\V\T\L\T\Y 66.7 6 6 0 0 0 b-ion bias 85.463661 1.533809 1052.5849 1052.587 -0.0074 -7.0 -0.7999999999999998 4.0 2838.3 nuORFdb_human ENST00000633556.1_1_6:106975319-106978099:+ ENST00000633556.1_1_6:106975319-106978099:+ AIM1 Out-of-Frame Out-of-Frame GN=AIM1 Out-of-Frame E_20221201_NO30_400nL_HLAc1_2e7_directIP_titration_rep1_Slot2-4_1_3515 20mio-rep3 E_20221201_NO30_400nL_HLAc1_2e7_directIP_titration_rep4_Slot2-4_1_3519.24793.309.1 1 1 1 9.62 4.26 4.81 5.35 75.5 6 0 0 2760.0 Hydrogen 10 (R) 9 ITELVTLTY (L) 9 LDITTLITY I T E\L\V\T|L|T\Y 66.7 6 6 2 2 0 b-ion bias 85.62571 1.539255 1052.5826 1052.587 -0.0074 -7.0 -1.2000000000000002 4.0 2838.3 nuORFdb_human ENST00000633556.1_1_6:106975319-106978099:+ ENST00000633556.1_1_6:106975319-106978099:+ AIM1 Out-of-Frame Out-of-Frame GN=AIM1 Out-of-Frame E_20221201_NO30_400nL_HLAc1_2e7_directIP_titration_rep4_Slot2-4_1_3519 40mio-rep2 E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep2_Slot2-5_1_3520.24749.303.1 1 2 1 9.63 4.49 3.48 5.14 75.9 6 0 0 7930.0 Hydrogen 10 (R) 9 ITELVTLTY (L) 9 TLLTLELSY I T E\L|V\T\L\T\Y 66.7 6 6 1 1 0 b-ion bias 85.494461 1.542429 1052.5759 1052.587 -0.0074 -7.0 -2.0999999999999996 4.0 2838.3 nuORFdb_human ENST00000633556.1_1_6:106975319-106978099:+ ENST00000633556.1_1_6:106975319-106978099:+ AIM1 Out-of-Frame Out-of-Frame GN=AIM1 Out-of-Frame E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep2_Slot2-5_1_3520 10mio-rep3 E_20221201_NO30_400nL_HLAc1_1e7_directIP_titration_rep3_Slot2-3_1_3514.16435.301.1 1 1 1 10.95 3.72 4.09 7.23 79.3 6 0 0 1370.0 Hydrogen 15 (R) 9 ITEVQQELY (-) 9 FHKTGQEIY I T E/V/Q|Q\E\L\Y 66.7 6 4 3 1 0 unbiased 56.773426 1.54373 1122.5601 1122.568 -0.0077 -6.9 -0.6000000000000005 3.8 2688.0 nuORFdb_human ENST00000262646.11_1_8:61484667-61496833:+ ENST00000262646.11_1_8:61484667-61496833:+ RAB2A Out-of-Frame Out-of-Frame GN=RAB2A Out-of-Frame E_20221201_NO30_400nL_HLAc1_1e7_directIP_titration_rep3_Slot2-3_1_3514 40mio-rep2 E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep2_Slot2-5_1_3520.16362.288.1 1 3 1 16.38 5.72 5.02 10.66 94.6 6 0 0 15800.0 Hydrogen 15 (R) 9 ITEVQQELY (-) 9 LTEQDLQLY I T E|V|Q|Q\E\L\Y 66.7 6 6 3 3 0 dual b/y bias 56.519657 1.551487 1122.5634 1122.568 -0.0077 -6.9 -2.0 3.8 2688.0 nuORFdb_human ENST00000262646.11_1_8:61484667-61496833:+ ENST00000262646.11_1_8:61484667-61496833:+ RAB2A Out-of-Frame Out-of-Frame GN=RAB2A Out-of-Frame E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep2_Slot2-5_1_3520 5mio-rep1 E_20221201_NO30_400nL_HLAc1_5e6_directIP_titration_rep1_Slot1-37_1_3502.16765.292.1 1 1 1 16.05 6.1 4.81 9.95 95.2 6 0 0 1580.0 Hydrogen 15 (R) 9 ITEVQQELY (-) 9 LTEQDLQLY I T E|V|Q|Q\E\L\Y 66.7 6 6 3 3 0 dual b/y bias 57.910099 1.549243 1122.5631 1122.568 -0.0077 -6.9 0.09999999999999964 3.8 2688.0 nuORFdb_human ENST00000262646.11_1_8:61484667-61496833:+ ENST00000262646.11_1_8:61484667-61496833:+ RAB2A Out-of-Frame Out-of-Frame GN=RAB2A Out-of-Frame E_20221201_NO30_400nL_HLAc1_5e6_directIP_titration_rep1_Slot1-37_1_3502 40mio-rep1 E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep1_Slot1-04_1_3496.27147.1306.2 2 1 1 14.5 5.42 8.72 9.08 87.7 7 0 1 5370.0 Hydrogen 35 (L) 10 ITSWFVPRPW (-) 10 LTREPHPWRP I/T/S/W|F/V|P-R/P W 80.0 8 2 7 2 1 y-ion bias 93.785492 0.93505 644.8433 1288.684 -0.005 -3.9 -0.6999999999999997 9.75 4837.6 nuORFdb_human ENST00000372209.3_1_1:45241798-45243366:+ ENST00000372209.3_1_1:45241798-45243366:+ RPS8 Out-of-Frame Out-of-Frame GN=RPS8 Out-of-Frame E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep1_Slot1-04_1_3496 40mio-rep2 E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep2_Slot2-5_1_3520.26940.1329.2 2 2 1 9.48 3.4 6.9 6.07 77.3 6 0 1 5880.0 Hydrogen 35 (L) 10 ITSWFVPRPW (-) 10 ITRSHSFnRAV I/T/S/W|F/V/P R P W 60.0 6 1 6 1 0 y-ion bias 93.065529 0.921013 644.8449 1288.684 -0.001 -0.8 4.1000000000000005 9.75 4837.6 nuORFdb_human ENST00000372209.3_1_1:45241798-45243366:+ ENST00000372209.3_1_1:45241798-45243366:+ RPS8 Out-of-Frame Out-of-Frame GN=RPS8 Out-of-Frame E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep2_Slot2-5_1_3520 40mio-rep1 E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep1_Slot1-04_1_3496.13201.1344.2 2 1 1 16.75 12.47 6.34 4.28 93.1 7 1 2 9780.0 Hydrogen 25 (M) 9 ITVLPKKGW (L) 9 TLVIVPRML I/T|V|L|P K/K\G-W 77.8 7 4 5 3 1 dual b/y bias 45.600788 0.911078 521.3256 1041.646 -0.0008 -0.8 2.4000000000000004 10.0 4217.1 smORF_v4|nuORFdb_human MTIF3_13:28024646-28014586:- MTIF3_13:28024646-28014586:-|ENST00000493719.5_1_13:28014569-28024213:-|ENST00000461838.5_1_13:28014569-28024229:-|ENST00000464753.1_1_13:28014569-28024306:-|ENST00000381120.7_1_13:28014569-28024670:-|ENST00000484342.2_1_13:28014569-28024692:- MTIF3 overlapping.uORF 13:28024646-28014586:- 13 MTIF3 5' Overlap uORF 5' Overlap uORF GN=MTIF3 E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep1_Slot1-04_1_3496 40mio-rep1 E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep1_Slot1-04_1_3496.30044.378.1 1 1 1 10.01 8.37 0.5 1.64 79.0 6 0 0 13200.0 Hydrogen 99 (G) 9 IVAGLVLLV (A) 9 VLLGLGLVL I V A\G\L\V\L\L\V 66.7 6 6 0 0 0 b-ion bias 103.791908 1.498424 896.6149 896.618 -0.0029 -3.3 -0.09999999999999964 5.52 16339.0 nuORFdb_human ENST00000383620.4_1_6:29857122-29858256:+ ENST00000383620.4_1_6:29857122-29858256:+ HLA-H Pseudogene Pseudogene GN=HLA-H Pseudogene E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep1_Slot1-04_1_3496 SCP E_20221201_NO30_400nL_HLAc1_4e7_PDAC_Slot2-6_1_3524.24960.215.1 1 1 1 13.85 3.81 8.29 10.04 92.3 6 0 0 4390.0 Hydrogen 54 (S) 9 IWCPCPPTF (M) 9 nSPEKCQGKE I W|C|P|C|P|P T\F 55.6 5 6 5 5 0 dual b/y bias Carbamidomethylation C:Carbamidomethylation, 86.229568 1.594292 1177.5084 1177.517 -0.0069 -5.9 -3.5000000000000004 5.52 8075.9 nuORFdb_human ENST00000373823.7_3_9:124043766-124064391:+ ENST00000373823.7_3_9:124043766-124064391:+ GSN 5' Overlap uORF 5' Overlap uORF GN=GSN 5' Overlap uORF E_20221201_NO30_400nL_HLAc1_4e7_PDAC_Slot2-6_1_3524 Eva-50mio-3fr AL20210827_EV_HLA_PDAC_PANFRO413_2D_50e6_W632_classI_fxn3.44902.45004.2 2 1 1 12.5 7.98 2.73 4.51 77.8 7 1 1 54400000.0 Hydrogen 36 (S) 9 IWDLSITRL (-) 9 LWLGLSLAPF I/W|D|L/S/I/T R-L 77.8 7 2 6 2 1 y-ion bias 0.998 75.0 81.3 59.11 0.0 558.8258 1116.641 0.0031 2.8 2.3 5.84 5143.8 smORF_v4|nuORFdb_human MGAT1_5:180235914-180236048:- MGAT1_5:180235914-180236048:-|ENST00000393340.7_1_5:180235916-180237137:-|ENST00000508702.1_1_5:180235916-180237505:-|ENST00000508090.1_1_5:180235916-180242424:-|ENST00000333055.8_1_5:180235916-180242536:- MGAT1 uORF 5:180235914-180236048:- 5 MGAT1 5' uORF 5' uORF GN=MGAT1 AL20210827_EV_HLA_PDAC_PANFRO413_2D_50e6_W632_classI_fxn3 Exploris AL20221201_MKP_PDAC_01.64316.64316.2 2 1 1 10.27 6.83 3.44 3.44 67.9 6 1 1 332000000.0 Hydrogen 36 (S) 9 IWDLSITRL (-) 9 IWDKAVVTGK I/W|D|L/S/I/T R L 66.7 6 2 6 2 0 y-ion bias 0.982 75.5 97.05 2.08 0.0 558.8251 1116.641 0.0017 1.5 0.7 5.84 5143.8 smORF_v4|nuORFdb_human MGAT1_5:180235914-180236048:- MGAT1_5:180235914-180236048:-|ENST00000393340.7_1_5:180235916-180237137:-|ENST00000508702.1_1_5:180235916-180237505:-|ENST00000508090.1_1_5:180235916-180242424:-|ENST00000333055.8_1_5:180235916-180242536:- MGAT1 uORF 5:180235914-180236048:- 5 MGAT1 5' uORF 5' uORF GN=MGAT1 AL20221201_MKP_PDAC_01 SCP E_20221201_NO30_400nL_HLAc1_4e7_PDAC_Slot2-6_1_3524.28252.187.1 1 1 1 12.32 6.37 4.43 5.95 68.0 7 1 1 27600.0 Hydrogen 36 (S) 9 IWDLSITRL (-) 9 EERISELKI I/W|D|L|S I|T\R\L 77.8 7 6 5 4 0 dual b/y bias 97.605614 1.610608 1116.64 1116.641 -0.0012 -1.0 1.4 5.84 5143.8 smORF_v4|nuORFdb_human MGAT1_5:180235914-180236048:- MGAT1_5:180235914-180236048:-|ENST00000393340.7_1_5:180235916-180237137:-|ENST00000508702.1_1_5:180235916-180237505:-|ENST00000508090.1_1_5:180235916-180242424:-|ENST00000333055.8_1_5:180235916-180242536:- MGAT1 uORF 5:180235914-180236048:- 5 MGAT1 5' uORF 5' uORF GN=MGAT1 E_20221201_NO30_400nL_HLAc1_4e7_PDAC_Slot2-6_1_3524 SCP E_20221201_NO30_400nL_HLAc1_4e7_PDAC_Slot2-6_1_3524.14464.1364.2 2 1 1 9.21 5.84 4.92 3.36 51.0 7 1 1 8750.0 Hydrogen 23 (L) 9 IYCTRLSSL (-) 9 LYCLISGLSS I/Y|C|T R\L\S\S\L 77.8 7 6 3 2 0 b-ion bias Carbamidomethylation C:Carbamidomethylation, 49.967903 0.898734 556.7899 1112.577 -0.0041 -3.7 -1.3000000000000003 8.75 3500.0 nuORFdb_human TCONS_00016342_9:71911653-71911746:- TCONS_00016342_9:71911653-71911746:- linc-PRKACG-1 lincRNA lncRNA GN=linc-PRKACG-1 lincRNA E_20221201_NO30_400nL_HLAc1_4e7_PDAC_Slot2-6_1_3524 SCP E_20221201_NO30_400nL_HLAc1_4e7_PDAC_Slot2-6_1_3524.13902.1303.2 2 1 1 10.38 4.76 5.62 5.62 70.0 8 0 0 5770.0 Hydrogen 246 (I) 9 IYEYCTHEF (A) 9 LYETGGCDmSL I/Y|E|Y/C/T/H\E-F 100.0 9 3 6 2 1 y-ion bias Carbamidomethylation C:Carbamidomethylation, 48.027134 0.936795 631.261 1261.519 -0.0047 -3.7 -1.3000000000000003 4.51 29863.9 nuORFdb_human ENST00000544349.5_1_12:122735394-122750988:- ENST00000544349.5_1_12:122735394-122750988:- VPS33A 3' Overlap dORF 3' Overlap dORF GN=VPS33A 3' Overlap dORF E_20221201_NO30_400nL_HLAc1_4e7_PDAC_Slot2-6_1_3524 SCP E_20221201_NO30_400nL_HLAc1_4e7_PDAC_Slot2-6_1_3524.25796.216.1 1 2 1 12.22 4.97 8.9 7.25 80.2 7 0 0 12900.0 Hydrogen 98 (L) 9 IYIENTSLF (C) 9 LSDLQTYLF I Y\I|E|N\T\S\L\F 77.8 7 7 2 2 0 b-ion bias 89.117432 1.593405 1099.5645 1099.567 -0.007 -6.4 -4.0 4.0 24525.5 nuORFdb_human ENST00000535977.5_1_16:677627-678308:+ ENST00000535977.5_1_16:677627-678308:+ RAB40C 3' dORF 3' dORF GN=RAB40C 3' dORF E_20221201_NO30_400nL_HLAc1_4e7_PDAC_Slot2-6_1_3524 SCP E_20221201_NO30_400nL_HLAc1_4e7_PDAC_Slot2-6_1_3524.27491.1290.2 2 2 1 21.77 15.98 5.88 5.79 98.8 8 2 2 115000.0 Hydrogen 22 (D) 9 IYLRVQLKI (-) 9 YLRVQLIKI I/Y|L/R|V\Q|L|K\I 100.0 9 6 6 4 0 dual b/y bias 94.976532 0.945349 573.3725 1145.741 -0.0018 -1.5 0.8999999999999999 9.99 3590.4 nuORFdb_human ENST00000443024.6_1_9:95050481-95051592:- ENST00000443024.6_1_9:95050481-95051592:- IARS Out-of-Frame Out-of-Frame GN=IARS Out-of-Frame E_20221201_NO30_400nL_HLAc1_4e7_PDAC_Slot2-6_1_3524 Eva-50mio-3fr AL20210827_EV_HLA_PDAC_PANFRO413_2D_50e6_W632_classI_fxn3.56388.56388.2 2 1 1 15.62 7.36 10.59 8.26 96.3 7 0 0 7420000.0 Hydrogen 44 (A) 11 IYPPLLGALSF (I) 11 YPPLLGALSFI I Y|P P L\L-G\A\L/S/F 54.5 6 4 3 1 1 unbiased 0.966 88.5 101.47 32.26 0.0 595.8474 1190.682 0.0056 4.7 4.2 5.52 6097.9 smORF_v4|nuORFdb_human MLLT3_9:20622328-20622507:- MLLT3_9:20622328-20622507:-|ENST00000380338.8_1_9:20622330-20622507:- MLLT3 uORF 9:20622328-20622507:- 9 MLLT3 5' uORF 5' uORF GN=MLLT3 AL20210827_EV_HLA_PDAC_PANFRO413_2D_50e6_W632_classI_fxn3 Exploris AL20221201_MKP_PDAC_01.68390.68390.2 2 1 1 15.43 6.56 11.34 8.87 93.8 7 0 0 70300000.0 Hydrogen 44 (A) 11 IYPPLLGALSF (I) 11 YPPLLGALSFI I Y|P P L\L-G\A\L/S/F 54.5 6 4 3 1 1 unbiased 0.994 96.1 101.87 4.11 0.0 595.8446 1190.682 -0.0001 -0.1 -0.9 5.52 6097.9 smORF_v4|nuORFdb_human MLLT3_9:20622328-20622507:- MLLT3_9:20622328-20622507:-|ENST00000380338.8_1_9:20622330-20622507:- MLLT3 uORF 9:20622328-20622507:- 9 MLLT3 5' uORF 5' uORF GN=MLLT3 AL20221201_MKP_PDAC_01 SCP E_20221201_NO30_400nL_HLAc1_4e7_PDAC_Slot2-6_1_3524.30243.136.1 1 3 1 19.18 5.2 13.43 13.98 99.4 9 0 0 216000.0 Hydrogen 44 (A) 11 IYPPLLGALSF (I) 11 AALQnKVVALY I Y/P/P\L|L|G\A\L\S\F 81.8 9 7 4 2 0 unbiased 104.487556 1.640688 1190.679 1190.682 -0.002 -1.7 0.7 5.52 6097.9 smORF_v4|nuORFdb_human MLLT3_9:20622328-20622507:- MLLT3_9:20622328-20622507:-|ENST00000380338.8_1_9:20622330-20622507:- MLLT3 uORF 9:20622328-20622507:- 9 MLLT3 5' uORF 5' uORF GN=MLLT3 E_20221201_NO30_400nL_HLAc1_4e7_PDAC_Slot2-6_1_3524 Eva-50mio-3fr AL20210827_EV_HLA_PDAC_PANFRO413_2D_50e6_W632_classI_fxn2.16450.16450.3 3 1 1 14.41 10.04 14.41 88.5 7 1 1 7240000.0 Hydrogen 7 (L) 9 IYSNHEYKW (-) 9 I/Y/S N/H/E-Y/K/W 77.8 7 0 6 0 1 y-ion bias 0.995 95.9 31.63 100.0 0.0 413.8649 1239.579 0.001 0.8 0.5 6.75 1925.1 nuORFdb_human ENST00000371804.3_1_10:91152438-91162049:+ ENST00000371804.3_1_10:91152438-91162049:+ IFIT1 5' Overlap uORF 5' Overlap uORF GN=IFIT1 5' Overlap uORF AL20210827_EV_HLA_PDAC_PANFRO413_2D_50e6_W632_classI_fxn2 SCP E_20221201_NO30_400nL_HLAc1_4e7_PDAC_Slot2-6_1_3524.9254.1284.2 2 4 1 16.5 6.27 8.04 10.23 89.9 7 1 1 71200.0 Hydrogen 7 (L) 9 IYSNHEYKW (-) 9 IGnHASYYKDA I/Y|S N\H/E|Y|K\W 77.8 7 5 5 3 0 dual b/y bias 31.969412 0.948708 620.2909 1239.579 -0.0046 -3.7 -1.3000000000000003 6.75 1925.1 nuORFdb_human ENST00000371804.3_1_10:91152438-91162049:+ ENST00000371804.3_1_10:91152438-91162049:+ IFIT1 5' Overlap uORF 5' Overlap uORF GN=IFIT1 5' Overlap uORF E_20221201_NO30_400nL_HLAc1_4e7_PDAC_Slot2-6_1_3524 Exploris_FAIMS VH20221207_MKP_FAIMS2022E_HLAc1_4e7_PDAC.26641.26641.2 2 2 1 15.32 15.32 15.32 83.2 7 1 1 17400000.0 Hydrogen 7 (L) 9 IYSNHEYKW (-) 9 I/Y|S N/H-E\Y/K/W 77.8 7 2 5 1 1 y-ion bias 0.941 71.2 53.68 120.0 -50.0 620.2904 1239.579 -0.0057 -4.6 -1.9999999999999996 6.75 1925.1 nuORFdb_human ENST00000371804.3_1_10:91152438-91162049:+ ENST00000371804.3_1_10:91152438-91162049:+ IFIT1 5' Overlap uORF 5' Overlap uORF GN=IFIT1 5' Overlap uORF VH20221207_MKP_FAIMS2022E_HLAc1_4e7_PDAC SCP E_20221201_NO30_400nL_HLAc1_4e7_PDAC_Slot2-6_1_3524.25348.1348.2 2 5 1 9.67 6.88 3.34 2.79 55.1 7 1 1 82500.0 Hydrogen 1 (-) 9 IYSTLWEKI (-) 9 YLNmLLEKL I/Y|S|T/L W|E\K\I 77.8 7 5 5 3 0 dual b/y bias 87.569534 0.908791 576.8178 1152.63 -0.0012 -1.1 1.2999999999999998 6.0 1152.3 nuORFdb_human ENST00000404125.5_1_2:54176390-54176420:- ENST00000404125.5_1_2:54176390-54176420:- PSME4 Out-of-Frame Out-of-Frame GN=PSME4 Out-of-Frame E_20221201_NO30_400nL_HLAc1_4e7_PDAC_Slot2-6_1_3524 40mio-rep1 E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep1_Slot1-04_1_3496.27516.1363.2 2 1 1 11.3 3.33 3.66 7.97 83.7 6 1 1 10600.0 Hydrogen 36 (M) 9 KALLIPYVW (F) 9 KLIIAmLER K A L\L|I|P|Y|V\W 66.7 6 6 4 4 0 dual b/y bias 95.060417 0.899574 551.8336 1102.666 -0.0052 -4.7 -1.5 8.59 7902.9 nuORFdb_human ENST00000602430.1_1_3:51575458-51575656:+ ENST00000602430.1_1_3:51575458-51575656:+ RAD54L2 ncRNA Retained Intron lncRNA GN=RAD54L2 ncRNA Retained Intron E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep1_Slot1-04_1_3496 40mio-rep2 E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep2_Slot2-5_1_3520.27336.1365.2 2 1 1 8.42 2.94 1.99 5.48 78.7 5 1 1 4380.0 Hydrogen 36 (M) 9 KALLIPYVW (F) 9 VVKVIGRGAFG K A L L\I|P/Y|V\W 55.6 5 4 3 2 0 unbiased 94.433601 0.898044 551.8337 1102.666 -0.0052 -4.7 0.20000000000000018 8.59 7902.9 nuORFdb_human ENST00000602430.1_1_3:51575458-51575656:+ ENST00000602430.1_1_3:51575458-51575656:+ RAD54L2 ncRNA Retained Intron lncRNA GN=RAD54L2 ncRNA Retained Intron E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep2_Slot2-5_1_3520 40mio-rep3 E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep3_Slot2-5_1_3521.27344.1370.2 2 1 1 10.12 3.15 3.75 6.97 82.2 5 1 1 4180.0 Hydrogen 36 (M) 9 KALLIPYVW (F) 9 KLIIAmLER K A L L|I|P\Y|V\W 55.6 5 5 3 3 0 dual b/y bias 94.453781 0.895156 551.8336 1102.666 -0.0052 -4.7 -0.10000000000000053 8.59 7902.9 nuORFdb_human ENST00000602430.1_1_3:51575458-51575656:+ ENST00000602430.1_1_3:51575458-51575656:+ RAD54L2 ncRNA Retained Intron lncRNA GN=RAD54L2 ncRNA Retained Intron E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep3_Slot2-5_1_3521 10mio-rep1 E_20221201_NO30_400nL_HLAc1_1e7_directIP_titration_rep1_Slot2-3_1_3512.14400.1467.2 2 1 1 10.39 9.25 2.64 1.14 82.6 5 1 1 4920.0 Hydrogen 21 (M) 9 KEESPVVSW (R) 9 RWGLDVSW K E E/S|P V|V|S\W 44.4 4 4 4 3 0 dual b/y bias 49.745823 0.834064 530.7659 1060.531 -0.0062 -5.9 1.7999999999999998 4.53 6212.1 nuORFdb_human ENST00000474011.5_1_21:11060978-11098717:- ENST00000474011.5_1_21:11060978-11098717:-|ENST00000624155.2_1_GL000237.1:901-2487:-|ENST00000412295.2_1_5:180710378-180710423:+ BAGE2 Pseudogene Pseudogene GN=BAGE2 Pseudogene E_20221201_NO30_400nL_HLAc1_1e7_directIP_titration_rep1_Slot2-3_1_3512 10mio-rep2 E_20221201_NO30_400nL_HLAc1_1e7_directIP_titration_rep2_Slot2-3_1_3513.14362.1471.2 2 1 1 10.94 7.28 3.6 3.66 81.2 6 1 1 5710.0 Hydrogen 21 (M) 9 KEESPVVSW (R) 9 DVGADLVVSW K E E/S|P\V\V|S\W 66.7 6 5 3 2 0 unbiased 49.614407 0.832037 530.7656 1060.531 -0.0062 -5.9 0.7999999999999998 4.53 6212.1 nuORFdb_human ENST00000474011.5_1_21:11060978-11098717:- ENST00000474011.5_1_21:11060978-11098717:-|ENST00000624155.2_1_GL000237.1:901-2487:-|ENST00000412295.2_1_5:180710378-180710423:+ BAGE2 Pseudogene Pseudogene GN=BAGE2 Pseudogene E_20221201_NO30_400nL_HLAc1_1e7_directIP_titration_rep2_Slot2-3_1_3513 10mio-rep3 E_20221201_NO30_400nL_HLAc1_1e7_directIP_titration_rep3_Slot2-3_1_3514.14323.1480.2 2 1 1 12.66 9.01 3.63 3.65 88.6 5 1 1 5660.0 Hydrogen 21 (M) 9 KEESPVVSW (R) 9 AGTPEITVSW K E E|S|P V|V|S\W 44.4 4 5 4 4 0 dual b/y bias 49.481823 0.825819 530.7639 1060.531 -0.0102 -9.6 -3.3 4.53 6212.1 nuORFdb_human ENST00000474011.5_1_21:11060978-11098717:- ENST00000474011.5_1_21:11060978-11098717:-|ENST00000624155.2_1_GL000237.1:901-2487:-|ENST00000412295.2_1_5:180710378-180710423:+ BAGE2 Pseudogene Pseudogene GN=BAGE2 Pseudogene E_20221201_NO30_400nL_HLAc1_1e7_directIP_titration_rep3_Slot2-3_1_3514 40mio-rep1 E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep1_Slot1-04_1_3496.14610.1441.2 2 2 1 11.61 8.41 3.52 3.2 77.4 6 1 1 45500.0 Hydrogen 21 (M) 9 KEESPVVSW (R) 9 AGTPEITVSW K E E\S|P\V|V|S\W 66.7 6 6 3 3 0 dual b/y bias 50.467297 0.850579 530.7682 1060.531 -0.0022 -2.1 1.1 4.53 6212.1 nuORFdb_human ENST00000474011.5_1_21:11060978-11098717:- ENST00000474011.5_1_21:11060978-11098717:-|ENST00000624155.2_1_GL000237.1:901-2487:-|ENST00000412295.2_1_5:180710378-180710423:+ BAGE2 Pseudogene Pseudogene GN=BAGE2 Pseudogene E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep1_Slot1-04_1_3496 40mio-rep3 E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep3_Slot2-5_1_3521.14273.1463.2 2 1 1 14.64 9.95 4.51 4.69 89.6 6 1 1 12300.0 Hydrogen 21 (M) 9 KEESPVVSW (R) 9 AGTPEITVSW K E E|S|P\V|V|S\W 66.7 6 6 4 4 0 dual b/y bias 49.308109 0.836933 530.7673 1060.531 -0.0042 -4.0 0.5999999999999996 4.53 6212.1 nuORFdb_human ENST00000474011.5_1_21:11060978-11098717:- ENST00000474011.5_1_21:11060978-11098717:-|ENST00000624155.2_1_GL000237.1:901-2487:-|ENST00000412295.2_1_5:180710378-180710423:+ BAGE2 Pseudogene Pseudogene GN=BAGE2 Pseudogene E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep3_Slot2-5_1_3521 40mio-rep1 E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep1_Slot1-04_1_3496.25873.1285.2 2 2 1 12.46 4.49 6.77 7.97 76.3 6 1 1 7910.0 Hydrogen 38 (A) 9 KEFFENWNW (R) 9 nAKEFFENWN K E F\F|E|N|W|N\W 66.7 6 6 4 4 0 dual b/y bias 89.38018 0.947973 650.2899 1299.579 -0.0066 -5.1 -1.8999999999999995 4.53 10843.3 nuORFdb_human ENST00000427574.6_1_11:76156149-76164398:+ ENST00000427574.6_1_11:76156149-76164398:+ EMSY 5' Overlap uORF 5' Overlap uORF GN=EMSY 5' Overlap uORF E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep1_Slot1-04_1_3496 40mio-rep1 E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep1_Slot1-04_1_3496.13423.1338.2 2 1 1 7.47 3.84 3.56 3.63 61.7 6 2 2 16300.0 Hydrogen 12 (E) 9 KEGEWKDVW (I) 9 EKTEWGLQEG K E/G E/W/K\D\V\W 55.6 5 3 3 0 0 unbiased 46.367294 0.915369 588.7866 1176.568 -0.0017 -1.4 1.8000000000000003 4.68 10033.2 nuORFdb_human ENST00000547963.1_1_12:114184084-114184339:- ENST00000547963.1_1_12:114184084-114184339:- LINC01234 lincRNA lncRNA GN=LINC01234 lincRNA E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep1_Slot1-04_1_3496 40mio-rep1 E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep1_Slot1-04_1_3496.9346.1304.2 2 2 1 7.97 4.9 2.14 3.07 56.8 6 2 2 41900.0 Hydrogen 23 (M) 10 KEQCGRSVLW (M) 10 LSNASCVGGVLW K/E/Q|C G R S-V|L\W 60.0 6 3 4 2 1 unbiased Carbamidomethylation C:Carbamidomethylation, 32.288307 0.936234 631.8179 1262.631 -0.0023 -1.8 1.4000000000000001 8.75 5826.7 nuORFdb_human ENST00000370873.8_1_20:60714937-60715996:- ENST00000370873.8_1_20:60714937-60715996:- PSMA7 Out-of-Frame Out-of-Frame GN=PSMA7 Out-of-Frame E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep1_Slot1-04_1_3496 40mio-rep2 E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep2_Slot2-5_1_3520.9043.1320.2 2 2 1 8.57 2.7 5.88 5.88 57.7 7 2 2 13200.0 Hydrogen 23 (M) 10 KEQCGRSVLW (M) 10 QKERnCKLW K/E/Q/C|G R S\V|L\W 70.0 7 4 5 2 0 unbiased Carbamidomethylation C:Carbamidomethylation, 31.240871 0.926576 631.8167 1262.631 -0.0043 -3.4 1.5000000000000004 8.75 5826.7 nuORFdb_human ENST00000370873.8_1_20:60714937-60715996:- ENST00000370873.8_1_20:60714937-60715996:- PSMA7 Out-of-Frame Out-of-Frame GN=PSMA7 Out-of-Frame E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep2_Slot2-5_1_3520 40mio-rep1 E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep1_Slot1-04_1_3496.10871.1577.3 3 1 1 12.21 5.62 5.63 6.59 77.9 7 3 3 66100.0 Hydrogen 47 (M) 10 KEWLQTKERW (K) 10 AnLDKnKQTLEK K/E/W|L/Q/T|K/E-R W 80.0 8 2 7 2 1 y-ion bias 37.554092 0.76468 468.5843 1403.743 -0.0056 -4.0 -0.7999999999999998 8.59 7986.1 nuORFdb_human ENST00000425011.1_3_X:102840593-102841700:+ ENST00000425011.1_3_X:102840593-102841700:+|ENST00000494801.5_1_X:102840495-102841700:+ TCEAL4 5' Overlap uORF 5' Overlap uORF GN=TCEAL4 5' Overlap uORF E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep1_Slot1-04_1_3496 40mio-rep2 E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep2_Slot2-5_1_3520.10580.1589.3 3 1 1 8.19 4.73 4.07 3.45 63.0 6 3 3 10500.0 Hydrogen 47 (M) 10 KEWLQTKERW (K) 10 KEKNGGARLGmAGV K/E/W-L|Q|T/K E R W 60.0 6 2 5 2 1 y-ion bias 36.549892 0.75718 468.5833 1403.743 -0.0086 -6.1 -1.1999999999999993 8.59 7986.1 nuORFdb_human ENST00000425011.1_3_X:102840593-102841700:+ ENST00000425011.1_3_X:102840593-102841700:+|ENST00000494801.5_1_X:102840495-102841700:+ TCEAL4 5' Overlap uORF 5' Overlap uORF GN=TCEAL4 5' Overlap uORF E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep2_Slot2-5_1_3520 Exploris AL20221201_MKP_PDAC_01.61815.61815.2 2 1 1 14.26 9.96 4.8 4.3 87.0 7 2 2 55200000.0 Hydrogen 53 (M) 9 KFITRQLLF (-) 9 KLNKKmKLF K/F/I/T R-Q\L|L|F 77.8 7 3 5 2 1 unbiased 0.988 83.2 93.4 67.11 0.0 583.3582 1165.709 -0.0001 -0.1 -0.9 11.0 7177.5 nuORFdb_human ENST00000341170.4_1_9:100388110-100389693:- ENST00000341170.4_1_9:100388110-100389693:- TSTD2 Out-of-Frame Out-of-Frame GN=TSTD2 Out-of-Frame AL20221201_MKP_PDAC_01 Exploris_FAIMS VH20221207_MKP_FAIMS2022E_HLAc1_4e7_PDAC.63259.63259.2 2 1 1 13.82 8.47 5.35 5.35 82.4 6 2 2 24200000.0 Hydrogen 53 (M) 9 KFITRQLLF (-) 9 KIYLVGRGLF K/F|I/T R Q\L|L|F 66.7 6 4 5 3 0 dual b/y bias 0.99 87.4 108.33 15.52 -50.0 583.3567 1165.709 -0.0031 -2.7 -0.10000000000000009 11.0 7177.5 nuORFdb_human ENST00000341170.4_1_9:100388110-100389693:- ENST00000341170.4_1_9:100388110-100389693:- TSTD2 Out-of-Frame Out-of-Frame GN=TSTD2 Out-of-Frame VH20221207_MKP_FAIMS2022E_HLAc1_4e7_PDAC Eva-50mio-3fr AL20210827_EV_HLA_PDAC_PANFRO413_2D_50e6_W632_classI_fxn3.31521.31521.2 2 1 1 9.53 2.67 5.72 6.86 66.2 6 1 1 6860000.0 Hydrogen 3 (N) 9 KFLVQNNVF (Q) 9 RKELGPYVF K F L\V\Q\N\N\V|F 66.7 6 6 1 1 0 b-ion bias 0.981 72.6 57.87 76.01 0.0 554.8117 1108.615 0.0012 1.1 0.6000000000000001 8.75 4796.3 nuORFdb_human ENST00000314367.14_1_9:175738-177793:- ENST00000314367.14_1_9:175738-177793:- CBWD1 3' Overlap dORF 3' Overlap dORF GN=CBWD1 3' Overlap dORF AL20210827_EV_HLA_PDAC_PANFRO413_2D_50e6_W632_classI_fxn3 40mio-rep1 E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep1_Slot1-04_1_3496.28691.1379.2 2 1 1 12.7 11.43 -0.06 1.27 90.1 5 1 1 12600.0 Hydrogen 5 (I) 10 KGQLLAVLQL (E) 10 RLnLVILQL K G Q L L-A|V|L|Q\L 50.0 5 4 3 3 1 unbiased 99.118729 0.889708 541.8475 1082.693 -0.0065 -6.0 -2.8 8.75 1725.1 nuORFdb_human ENST00000442940.1_1_X:7894156-7895742:- ENST00000442940.1_1_X:7894156-7895742:- PNPLA4 5' Overlap uORF 5' Overlap uORF GN=PNPLA4 5' Overlap uORF E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep1_Slot1-04_1_3496 10mio-rep1 E_20221201_NO30_400nL_HLAc1_1e7_directIP_titration_rep1_Slot2-3_1_3512.9480.1485.2 2 2 1 13.78 6.17 5.27 7.61 79.2 6 1 1 7640.0 M34m Hydrogen 28 (L) 9 KIQGTLmTV (M) 9 IGISAKAmSI K I/Q|G T\L|m\T\V 55.6 5 5 3 2 0 unbiased m:Oxidized methionine 32.75301 0.82328 503.7791 990.565 15.9855 -9.4 -1.7000000000000002 8.75 6876.0 nuORFdb_human ENST00000586602.5_1_19:9677598-9677772:- ENST00000586602.5_1_19:9677598-9677772:- ZNF121 Out-of-Frame Out-of-Frame GN=ZNF121 Out-of-Frame E_20221201_NO30_400nL_HLAc1_1e7_directIP_titration_rep1_Slot2-3_1_3512 10mio-rep2 E_20221201_NO30_400nL_HLAc1_1e7_directIP_titration_rep2_Slot2-3_1_3513.9433.1487.2 2 1 1 16.01 9.3 4.73 6.71 84.2 6 1 1 4060.0 M34m Hydrogen 28 (L) 9 KIQGTLmTV (M) 9 KTNIAmLTV K I/Q|G T|L\m|T\V 55.6 5 5 4 3 0 dual b/y bias m:Oxidized methionine 32.589928 0.821608 503.7793 990.565 15.9855 -9.4 -2.7 8.75 6876.0 nuORFdb_human ENST00000586602.5_1_19:9677598-9677772:- ENST00000586602.5_1_19:9677598-9677772:- ZNF121 Out-of-Frame Out-of-Frame GN=ZNF121 Out-of-Frame E_20221201_NO30_400nL_HLAc1_1e7_directIP_titration_rep2_Slot2-3_1_3513 10mio-rep3 E_20221201_NO30_400nL_HLAc1_1e7_directIP_titration_rep3_Slot2-3_1_3514.9438.1501.2 2 1 1 12.57 5.11 4.61 7.46 72.3 6 1 1 3260.0 M34m Hydrogen 28 (L) 9 KIQGTLmTV (M) 9 mLSVInLTV K I/Q|G T/L|m\T\V 55.6 5 4 4 2 0 unbiased m:Oxidized methionine 32.609661 0.812601 503.7807 990.565 15.9895 -5.4 0.8999999999999995 8.75 6876.0 nuORFdb_human ENST00000586602.5_1_19:9677598-9677772:- ENST00000586602.5_1_19:9677598-9677772:- ZNF121 Out-of-Frame Out-of-Frame GN=ZNF121 Out-of-Frame E_20221201_NO30_400nL_HLAc1_1e7_directIP_titration_rep3_Slot2-3_1_3514 20mio-rep3 E_20221201_NO30_400nL_HLAc1_2e7_directIP_titration_rep4_Slot2-4_1_3519.9411.1483.2 2 2 1 16.72 7.01 6.61 9.7 83.6 6 1 1 9410.0 M34m Hydrogen 28 (L) 9 KIQGTLmTV (M) 9 KTNIAmLTV K I|Q|G T|L|m\T\V 55.6 5 6 4 4 0 dual b/y bias m:Oxidized methionine 32.511353 0.824534 503.7811 990.565 15.9895 -5.4 0.39999999999999947 8.75 6876.0 nuORFdb_human ENST00000586602.5_1_19:9677598-9677772:- ENST00000586602.5_1_19:9677598-9677772:- ZNF121 Out-of-Frame Out-of-Frame GN=ZNF121 Out-of-Frame E_20221201_NO30_400nL_HLAc1_2e7_directIP_titration_rep4_Slot2-4_1_3519 40mio-rep1 E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep1_Slot1-04_1_3496.9653.1452.2 2 1 1 16.85 8.13 4.99 8.72 84.8 6 1 1 12900.0 M34m Hydrogen 28 (L) 9 KIQGTLmTV (M) 9 IAmSIKAGTV K I/Q|G T|L|m\T\V 55.6 5 5 4 3 0 dual b/y bias m:Oxidized methionine 33.347862 0.843598 503.7817 990.565 15.9915 -3.4 -0.19999999999999973 8.75 6876.0 nuORFdb_human ENST00000586602.5_1_19:9677598-9677772:- ENST00000586602.5_1_19:9677598-9677772:- ZNF121 Out-of-Frame Out-of-Frame GN=ZNF121 Out-of-Frame E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep1_Slot1-04_1_3496 40mio-rep2 E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep2_Slot2-5_1_3520.9368.1469.2 2 2 1 15.85 7.08 5.45 8.77 79.7 7 1 1 20900.0 M34m Hydrogen 28 (L) 9 KIQGTLmTV (M) 9 KTNIAmLTV K I/Q|G\T|L|m|T\V 77.8 7 6 5 4 0 dual b/y bias m:Oxidized methionine 32.363598 0.83274 503.7814 990.565 15.9895 -5.4 -0.5 8.75 6876.0 nuORFdb_human ENST00000586602.5_1_19:9677598-9677772:- ENST00000586602.5_1_19:9677598-9677772:- ZNF121 Out-of-Frame Out-of-Frame GN=ZNF121 Out-of-Frame E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep2_Slot2-5_1_3520 40mio-rep4 E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep4_Slot2-5_1_3522.9385.1477.2 2 1 1 13.8 8.2 4.1 5.6 75.1 6 1 1 14600.0 M34m Hydrogen 28 (L) 9 KIQGTLmTV (M) 9 KTNIAmLTV K I/Q|G T|L|m\T\V 55.6 5 5 4 3 0 dual b/y bias m:Oxidized methionine 32.413494 0.828257 503.7809 990.565 15.9895 -5.4 -0.7000000000000002 8.75 6876.0 nuORFdb_human ENST00000586602.5_1_19:9677598-9677772:- ENST00000586602.5_1_19:9677598-9677772:- ZNF121 Out-of-Frame Out-of-Frame GN=ZNF121 Out-of-Frame E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep4_Slot2-5_1_3522 40mio-rep1 E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep1_Slot1-04_1_3496.15717.1313.2 2 1 1 14.21 1.99 9.81 12.22 83.9 7 1 1 7670.0 Hydrogen 28 (S) 10 KIVNVETQEW (A) 10 TVKVLGQGEEW K I V|N\V|E|T|Q|E\W 70.0 7 7 5 5 0 dual b/y bias 54.290852 0.931006 623.3275 1245.647 -0.0007 -0.5 2.7 4.53 7521.7 smORF_v4 CTC-480C2.1_5:84959484-84959684:+ CTC-480C2.1_5:84959484-84959684:+ CTC-480C2.1 lncRNA 5:84959484-84959684:+ 5 lincRNA GN=CTC-480C2.1 E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep1_Slot1-04_1_3496 Eva-50mio-3fr AL20210827_EV_HLA_PDAC_PANFRO413_2D_50e6_W632_classI_fxn3.27668.27668.2 2 1 1 11.21 8.03 4.12 3.19 75.2 7 2 2 2830000.0 Hydrogen 18 (S) 9 KIWERIWSA (S) 9 KILERWWGT K/I|W/E/R\I\W/S A 77.8 7 3 5 1 0 unbiased 0.906 64.1 51.13 100.0 0.0 594.8299 1188.652 0.0001 0.1 -0.4 8.75 3725.2 nuORFdb_human ENST00000532383.5_1_11:10825070-10825157:- ENST00000532383.5_1_11:10825070-10825157:-|ENST00000339995.9_2_11:10825067-10825719:- EIF4G2 ncRNA Retained Intron lncRNA GN=EIF4G2 ncRNA Retained Intron AL20210827_EV_HLA_PDAC_PANFRO413_2D_50e6_W632_classI_fxn3 40mio-rep1 E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep1_Slot1-04_1_3496.25080.1403.2 2 2 1 11.56 5.78 6.36 5.79 81.0 6 1 1 20500.0 Hydrogen 107 (W) 9 KLAELFTSW (I) 9 AYVVVGITSW K L A\E|L\F|T|S\W 66.7 6 6 3 3 0 dual b/y bias 86.641556 0.874168 547.7951 1094.588 -0.0053 -4.9 -1.7000000000000002 6.0 14230.9 nuORFdb_human ENST00000512876.5_1_5:138629414-138653336:+ ENST00000512876.5_1_5:138629414-138653336:+ MATR3 5' Overlap uORF 5' Overlap uORF GN=MATR3 5' Overlap uORF E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep1_Slot1-04_1_3496 40mio-rep2 E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep2_Slot2-5_1_3520.24949.1423.2 2 2 1 11.78 6.1 5.68 5.68 83.4 6 1 1 11200.0 Hydrogen 107 (W) 9 KLAELFTSW (I) 9 AYVVVGITSW K L A\E|L\F|T|S\W 66.7 6 6 3 3 0 dual b/y bias 86.185738 0.862103 547.7948 1094.588 -0.0053 -4.9 0.0 6.0 14230.9 nuORFdb_human ENST00000512876.5_1_5:138629414-138653336:+ ENST00000512876.5_1_5:138629414-138653336:+ MATR3 5' Overlap uORF 5' Overlap uORF GN=MATR3 5' Overlap uORF E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep2_Slot2-5_1_3520 40mio-rep4 E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep4_Slot2-5_1_3522.25047.1425.2 2 1 1 8.59 3.87 5.77 4.71 65.4 6 1 1 6670.0 Hydrogen 107 (W) 9 KLAELFTSW (I) 9 FGLLSALTLC K L A|E|L\F|T|S\W 66.7 6 6 4 4 0 dual b/y bias 86.512978 0.860887 547.7957 1094.588 -0.0033 -3.1 1.6 6.0 14230.9 nuORFdb_human ENST00000512876.5_1_5:138629414-138653336:+ ENST00000512876.5_1_5:138629414-138653336:+ MATR3 5' Overlap uORF 5' Overlap uORF GN=MATR3 5' Overlap uORF E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep4_Slot2-5_1_3522 40mio-rep1 E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep1_Slot1-04_1_3496.6132.1495.2 2 1 1 7.54 5.14 2.4 2.4 74.1 5 2 2 9680.0 Hydrogen 31 (W) 9 KLAPQLRGA (-) 9 KLALKFHP K/L/A|P Q|L R\G A 33.3 3 3 4 2 0 unbiased 21.191711 0.816616 477.2958 953.589 -0.0044 -4.6 -1.3999999999999995 11.0 4395.1 nuORFdb_human ENST00000265990.10_1_10:93683679-93683796:+ ENST00000265990.10_1_10:93683679-93683796:+ BTAF1 5' uORF 5' uORF GN=BTAF1 5' uORF E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep1_Slot1-04_1_3496 20mio-rep1 E_20221201_NO30_400nL_HLAc1_2e7_directIP_titration_rep1_Slot2-4_1_3515.23881.1397.2 2 1 1 10.38 5.54 5.67 4.84 71.7 6 1 1 2290.0 Hydrogen 87 (L) 9 KLIQLQFEV (-) 9 LQKIIQFVE K L I\Q\L|Q|F\E\V 66.7 6 6 2 2 0 b-ion bias 82.467361 0.878321 559.3306 1117.662 -0.0068 -6.1 0.10000000000000053 6.0 10521.9 nuORFdb_human ENST00000393179.8_1_12:89885842-89919131:- ENST00000393179.8_1_12:89885842-89919131:-|ENST00000313546.7_1_12:89885839-89890951:- POC1B 3' Overlap dORF 3' Overlap dORF GN=POC1B 3' Overlap dORF E_20221201_NO30_400nL_HLAc1_2e7_directIP_titration_rep1_Slot2-4_1_3515 20mio-rep2 E_20221201_NO30_400nL_HLAc1_2e7_directIP_titration_rep3_Slot2-4_1_3518.23893.1382.2 2 1 1 10.37 4.95 6.06 5.41 76.4 6 1 1 2290.0 Hydrogen 87 (L) 9 KLIQLQFEV (-) 9 KLLQLQEVF K L I\Q\L|Q|F\E\V 66.7 6 6 2 2 0 b-ion bias 82.527969 0.887569 559.3302 1117.662 -0.0088 -7.9 -1.6000000000000005 6.0 10521.9 nuORFdb_human ENST00000393179.8_1_12:89885842-89919131:- ENST00000393179.8_1_12:89885842-89919131:-|ENST00000313546.7_1_12:89885839-89890951:- POC1B 3' Overlap dORF 3' Overlap dORF GN=POC1B 3' Overlap dORF E_20221201_NO30_400nL_HLAc1_2e7_directIP_titration_rep3_Slot2-4_1_3518 40mio-rep1 E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep1_Slot1-04_1_3496.24217.1361.2 2 2 1 15.52 8.27 9.23 7.26 92.6 6 1 1 24100.0 Hydrogen 87 (L) 9 KLIQLQFEV (-) 9 EFQLLLKGAV K L I|Q|L|Q|F\E\V 66.7 6 6 4 4 0 dual b/y bias 83.658432 0.900698 559.3327 1117.662 -0.0028 -2.5 0.7000000000000002 6.0 10521.9 nuORFdb_human ENST00000393179.8_1_12:89885842-89919131:- ENST00000393179.8_1_12:89885842-89919131:-|ENST00000313546.7_1_12:89885839-89890951:- POC1B 3' Overlap dORF 3' Overlap dORF GN=POC1B 3' Overlap dORF E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep1_Slot1-04_1_3496 40mio-rep2 E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep2_Slot2-5_1_3520.24111.1387.2 2 1 1 11.43 6.62 7.33 4.81 74.8 6 1 1 7640.0 Hydrogen 87 (L) 9 KLIQLQFEV (-) 9 KLLQLQEVF K L I|Q/L|Q|F\E\V 66.7 6 5 4 3 0 dual b/y bias 83.290756 0.884329 559.3316 1117.662 -0.0048 -4.3 0.6000000000000005 6.0 10521.9 nuORFdb_human ENST00000393179.8_1_12:89885842-89919131:- ENST00000393179.8_1_12:89885842-89919131:-|ENST00000313546.7_1_12:89885839-89890951:- POC1B 3' Overlap dORF 3' Overlap dORF GN=POC1B 3' Overlap dORF E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep2_Slot2-5_1_3520 SCP E_20221201_NO30_400nL_HLAc1_4e7_PDAC_Slot2-6_1_3524.27111.1376.2 2 1 1 7.39 4.29 0.53 3.1 71.3 5 2 2 7610.0 Hydrogen 22 (S) 9 KLLDFSTRI (-) 9 KILnEPLKH K/L/L|D|F/S T R I 55.6 5 2 5 2 0 y-ion bias 93.664429 0.891178 546.8228 1092.641 -0.0024 -2.2 0.19999999999999973 8.75 3592.0 nuORFdb_human ENST00000521797.1_1_5:162884004-162884578:- ENST00000521797.1_1_5:162884004-162884578:- NUDCD2 ncRNA Processed Transcript lncRNA GN=NUDCD2 ncRNA Processed Transcript E_20221201_NO30_400nL_HLAc1_4e7_PDAC_Slot2-6_1_3524 Exploris_FAIMS VH20221207_MKP_FAIMS2022E_HLAc1_4e7_PDAC.14377.14476.3 3 1 1 8.45 5.6 3.3 2.85 62.9 6 2 3 2400000.0 Hydrogen 6 (M) 9 KLPEKRATF (F) 9 KLPEIQHPK K/L/P/E/K/R A T/F 66.7 6 0 6 0 0 y-ion bias 0.988 94.7 31.15 96.71 -50.0 363.8857 1089.641 0.001 0.9 3.5 9.99 2882.4 nuORFdb_human ENST00000433259.6_3_20:25187179-25187745:+ ENST00000433259.6_3_20:25187179-25187745:+ ENTPD6 Out-of-Frame Out-of-Frame GN=ENTPD6 Out-of-Frame VH20221207_MKP_FAIMS2022E_HLAc1_4e7_PDAC SCP E_20221201_NO30_400nL_HLAc1_4e7_PDAC_Slot2-6_1_3524.2917.1715.3 3 1 1 10.6 9.02 1.13 1.58 81.1 6 3 3 110000.0 Hydrogen 1 (-) 8 KLRNPKSL (N) 8 LPKNRSLK K/L/R N\P\K\S\L 75.0 6 4 2 0 0 b-ion bias 10.088516 0.676976 319.205 955.605 -0.0043 -4.5 -2.1 11.17 3601.0 nuORFdb_human ENST00000476149.1_1_2:118588229-118588328:+ ENST00000476149.1_1_2:118588229-118588328:+ DDX18 ncRNA Retained Intron lncRNA GN=DDX18 ncRNA Retained Intron E_20221201_NO30_400nL_HLAc1_4e7_PDAC_Slot2-6_1_3524 Exploris_FAIMS VH20221207_MKP_FAIMS2022E_HLAc1_4e7_PDAC.12702.12795.2 2 1 1 15.25 13.2 3.29 2.06 87.2 5 3 3 4700000.0 Hydrogen 1 (-) 8 KLRNPKSL (N) 8 KPGRKGISL K/L/R N P\K|S|L 62.5 5 3 4 2 0 unbiased 0.996 96.8 27.58 120.0 -50.0 478.3043 955.605 -0.0034 -3.5 -0.8999999999999999 11.17 3601.0 nuORFdb_human ENST00000476149.1_1_2:118588229-118588328:+ ENST00000476149.1_1_2:118588229-118588328:+ DDX18 ncRNA Retained Intron lncRNA GN=DDX18 ncRNA Retained Intron VH20221207_MKP_FAIMS2022E_HLAc1_4e7_PDAC SCP E_20221201_NO30_400nL_HLAc1_4e7_PDAC_Slot2-6_1_3524.29562.1243.2 2 1 1 14.79 8.91 6.03 5.89 86.0 7 1 1 12300.0 Hydrogen 181 (Y) 11 KLWEIPSLAYL (I) 11 AEERLLAALAYL K L W\E/I\P S\L|A|Y\L 54.5 6 6 3 2 0 b-ion bias 102.13549 0.974007 666.8801 1332.756 -0.0035 -2.6 -0.20000000000000018 6.0 22195.5 nuORFdb_human ENST00000465772.5_1_10:27793310-27827017:+ ENST00000465772.5_1_10:27793310-27827017:+ RAB18 3' Overlap dORF 3' Overlap dORF GN=RAB18 3' Overlap dORF E_20221201_NO30_400nL_HLAc1_4e7_PDAC_Slot2-6_1_3524 SCP E_20221201_NO30_400nL_HLAc1_4e7_PDAC_Slot2-6_1_3524.28488.227.1 1 2 1 9.19 4.28 4.76 4.91 70.9 7 1 1 22000.0 Hydrogen 7 (M) 9 KLWTSLVNL (-) 9 VSGAKGSKINI K L/W|T\S\L\V\N\L 77.8 7 6 2 1 0 b-ion bias 98.420502 1.587076 1073.6332 1073.635 -0.0054 -5.0 -2.6 8.75 1766.1 nuORFdb_human ENST00000513643.5_1_4:2492220-2502421:+ ENST00000513643.5_1_4:2492220-2502421:+|ENST00000504224.5_3_4:2498845-2502421:+ RNF4 Out-of-Frame Out-of-Frame GN=RNF4 Out-of-Frame E_20221201_NO30_400nL_HLAc1_4e7_PDAC_Slot2-6_1_3524 10mio-rep3 E_20221201_NO30_400nL_HLAc1_1e7_directIP_titration_rep3_Slot2-3_1_3514.15508.1412.2 2 1 1 7.6 1.25 4.2 6.35 55.7 6 1 1 4060.0 M5m M10m Hydrogen 4 (S) 9 KmLSLQmTF (-) 9 mIPTNPPPTF K m L\S/L|Q|m|T\F 66.7 6 5 4 3 0 dual b/y bias m:Oxidized methionine 53.573372 0.869007 565.7793 1098.569 31.9821 -6.8 -0.5 8.75 1412.7 nuORFdb_human ENST00000465259.5_2_3:148802548-148802587:- ENST00000465259.5_2_3:148802548-148802587:- HLTF Out-of-Frame Out-of-Frame GN=HLTF Out-of-Frame E_20221201_NO30_400nL_HLAc1_1e7_directIP_titration_rep3_Slot2-3_1_3514 40mio-rep1 E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep1_Slot1-04_1_3496.15732.1368.2 2 1 1 8.48 2.05 4.41 6.43 63.2 6 1 1 14700.0 M5m M10m Hydrogen 4 (S) 9 KmLSLQmTF (-) 9 mIPTNPPPTF K m L\S\L\Q|m|T\F 66.7 6 6 2 2 0 b-ion bias m:Oxidized methionine 54.342651 0.896368 565.7807 1098.569 31.9861 -3.3 -0.09999999999999964 8.75 1412.7 nuORFdb_human ENST00000465259.5_2_3:148802548-148802587:- ENST00000465259.5_2_3:148802548-148802587:- HLTF Out-of-Frame Out-of-Frame GN=HLTF Out-of-Frame E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep1_Slot1-04_1_3496 Eva-50mio-3fr AL20210827_EV_HLA_PDAC_PANFRO413_2D_50e6_W632_classI_fxn2.22976.22976.2 2 1 1 10.93 6.45 4.53 4.47 72.9 5 0 1 13100000.0 Hydrogen 13 (G) 9 KPFPGTSEF (-) 9 QLRETSEF K P\F|P G T\S\E|F 44.4 4 5 2 2 0 b-ion bias 0.962 92.4 43.23 100.0 0.0 505.2526 1009.499 -0.001 -1.0 -1.3 6.0 2478.9 nuORFdb_human ENST00000381782.6_2_4:40752304-40752672:+ ENST00000381782.6_2_4:40752304-40752672:+ NSUN7 5' uORF 5' uORF GN=NSUN7 5' uORF AL20210827_EV_HLA_PDAC_PANFRO413_2D_50e6_W632_classI_fxn2 Exploris AL20221201_MKP_PDAC_01.29886.29886.2 2 1 1 8.69 5.38 3.5 3.31 54.1 5 0 1 561000000.0 Hydrogen 13 (G) 9 KPFPGTSEF (-) 9 KPFQnVEF K P\F|P G T\S\E|F 44.4 4 5 2 2 0 b-ion bias 0.995 70.8 48.4 29.26 0.0 505.2537 1009.499 0.0012 1.2 0.3999999999999999 6.0 2478.9 nuORFdb_human ENST00000381782.6_2_4:40752304-40752672:+ ENST00000381782.6_2_4:40752304-40752672:+ NSUN7 5' uORF 5' uORF GN=NSUN7 5' uORF AL20221201_MKP_PDAC_01 Exploris_FAIMS VH20221207_MKP_FAIMS2022E_HLAc1_4e7_PDAC.36462.36581.2 2 1 1 14.06 8.29 6.57 5.77 85.6 7 0 1 53600000.0 Hydrogen 13 (G) 9 KPFPGTSEF (-) 9 KPFQnVEF K P\F|P-G\T\S\E|F 77.8 7 6 2 2 1 b-ion bias 0.994 86.8 68.37 120.0 -50.0 505.2515 1009.499 -0.0032 -3.1 -0.5 6.0 2478.9 nuORFdb_human ENST00000381782.6_2_4:40752304-40752672:+ ENST00000381782.6_2_4:40752304-40752672:+ NSUN7 5' uORF 5' uORF GN=NSUN7 5' uORF VH20221207_MKP_FAIMS2022E_HLAc1_4e7_PDAC Exploris AL20221201_MKP_PDAC_01.35513.35513.2 2 1 1 10.19 4.71 10.19 60.6 7 0 1 30200000.0 Hydrogen 3 (G) 11 KPMEVFQSSSL (-) 11 K P M E-V\F\Q\S\S|S|L 63.6 7 6 2 2 1 b-ion bias 0.937 100.0 55.75 98.18 0.0 626.8168 1252.624 0.0021 1.7 0.8999999999999999 6.0 1440.7 nuORFdb_human ENST00000393766.6_1_4:166258954-166258996:+ ENST00000393766.6_1_4:166258954-166258996:+ MSMO1 5' uORF 5' uORF GN=MSMO1 5' uORF AL20221201_MKP_PDAC_01 SCP E_20221201_NO30_400nL_HLAc1_4e7_PDAC_Slot2-6_1_3524.16458.1288.2 2 2 1 16.3 9.06 7.25 7.25 80.1 7 0 1 51400.0 Hydrogen 3 (G) 11 KPMEVFQSSSL (-) 11 SLLCLLCSSSL K P M E/V|F|Q|S|S\S\L 63.6 7 6 5 4 0 dual b/y bias 56.857803 0.946603 626.815 1252.624 -0.0015 -1.2 1.2 6.0 1440.7 nuORFdb_human ENST00000393766.6_1_4:166258954-166258996:+ ENST00000393766.6_1_4:166258954-166258996:+ MSMO1 5' uORF 5' uORF GN=MSMO1 5' uORF E_20221201_NO30_400nL_HLAc1_4e7_PDAC_Slot2-6_1_3524 SCP E_20221201_NO30_400nL_HLAc1_4e7_PDAC_Slot2-6_1_3524.12796.1281.2 2 1 1 9.51 5.49 2.64 4.01 57.8 5 0 1 38500.0 M5m Hydrogen 3 (G) 11 KPmEVFQSSSL (-) 11 DSGRYSPLSSSL K P m E V|F|Q|S|S\S L 36.4 4 5 4 4 0 dual b/y bias m:Oxidized methionine 44.208138 0.950942 634.8119 1252.624 15.9925 -1.9 0.5 6.0 1440.7 nuORFdb_human ENST00000393766.6_1_4:166258954-166258996:+ ENST00000393766.6_1_4:166258954-166258996:+ MSMO1 5' uORF 5' uORF GN=MSMO1 5' uORF E_20221201_NO30_400nL_HLAc1_4e7_PDAC_Slot2-6_1_3524 SCP E_20221201_NO30_400nL_HLAc1_4e7_PDAC_Slot2-6_1_3524.3763.1721.3 3 1 1 8.98 4.86 4.12 4.12 66.3 6 0 1 5490.0 Hydrogen 18 (R) 9 KPTVHHLSV (-) 9 KNLMNVLSV K/P/T V H\H\L\S\V 66.7 6 4 2 0 0 b-ion bias 13.012284 0.673098 339.864 1017.584 -0.0065 -6.4 -4.0 8.76 2970.6 nuORFdb_human ENST00000296181.8_1_3:124578258-124592335:- ENST00000296181.8_1_3:124578258-124592335:- ITGB5 Out-of-Frame Out-of-Frame GN=ITGB5 Out-of-Frame E_20221201_NO30_400nL_HLAc1_4e7_PDAC_Slot2-6_1_3524 40mio-rep1 E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep1_Slot1-04_1_3496.16350.1379.2 2 1 1 11.05 6.11 6.12 4.93 68.3 7 2 2 21200.0 Hydrogen 143 (A) 9 KSFEAPIKW (I) 9 KIPAWAHPW K/S/F E|A\P\I|K\W 77.8 7 5 4 2 0 unbiased 56.478123 0.88978 553.3043 1105.604 -0.0033 -3.0 0.20000000000000018 8.59 28309.6 nuORFdb_human ENST00000460389.5_1_20:30136650-30157019:+ ENST00000460389.5_1_20:30136650-30157019:+ HM13 3' Overlap dORF 3' Overlap dORF GN=HM13 3' Overlap dORF E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep1_Slot1-04_1_3496 20mio-rep1 E_20221201_NO30_400nL_HLAc1_2e7_directIP_titration_rep1_Slot2-4_1_3515.19152.1539.2 2 1 1 11.39 8.99 1.68 2.4 85.0 6 1 1 3040.0 Hydrogen 77 (E) 9 KSISLQVAL (V) 9 LNISTKVAL K S I/S\L|Q\V\A\L 66.7 6 5 2 1 0 b-ion bias 66.141342 0.788695 479.7969 958.593 -0.0064 -6.7 -0.5 8.75 11141.4 nuORFdb_human ENST00000316059.6_1_11:58352348-58378460:+ ENST00000316059.6_1_11:58352348-58378460:+ ZFP91 Out-of-Frame Out-of-Frame GN=ZFP91 Out-of-Frame E_20221201_NO30_400nL_HLAc1_2e7_directIP_titration_rep1_Slot2-4_1_3515 20mio-rep3 E_20221201_NO30_400nL_HLAc1_2e7_directIP_titration_rep4_Slot2-4_1_3519.19206.1505.2 2 2 1 10.96 8.31 1.72 2.65 82.4 6 1 1 10000.0 Hydrogen 77 (E) 9 KSISLQVAL (V) 9 VTKALDKAL K S I/S\L|Q\V\A\L 66.7 6 5 2 1 0 b-ion bias 66.333755 0.810629 479.7968 958.593 -0.0064 -6.7 -0.9000000000000004 8.75 11141.4 nuORFdb_human ENST00000316059.6_1_11:58352348-58378460:+ ENST00000316059.6_1_11:58352348-58378460:+ ZFP91 Out-of-Frame Out-of-Frame GN=ZFP91 Out-of-Frame E_20221201_NO30_400nL_HLAc1_2e7_directIP_titration_rep4_Slot2-4_1_3519 40mio-rep1 E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep1_Slot1-04_1_3496.19322.1481.2 2 2 1 11.6 8.05 1.67 3.55 80.3 6 1 1 16300.0 Hydrogen 77 (E) 9 KSISLQVAL (V) 9 KMRLAVGAL K S I/S/L|Q\V\A\L 66.7 6 4 3 1 0 unbiased 66.748093 0.825498 479.7988 958.593 -0.0024 -2.5 0.7000000000000002 8.75 11141.4 nuORFdb_human ENST00000316059.6_1_11:58352348-58378460:+ ENST00000316059.6_1_11:58352348-58378460:+ ZFP91 Out-of-Frame Out-of-Frame GN=ZFP91 Out-of-Frame E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep1_Slot1-04_1_3496 40mio-rep3 E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep3_Slot2-5_1_3521.8039.1502.2 2 1 1 7.92 7.92 7.92 63.7 6 1 2 10800.0 Hydrogen 15 (E) 9 KSKPLVGSF (-) 9 K/S K|P L\V\G|S\F 55.6 5 5 3 2 0 unbiased 27.771917 0.812366 481.7845 962.567 -0.0062 -6.5 -1.9000000000000004 10.0 2477.9 nuORFdb_human ENST00000371084.7_1_1:64095120-64095192:+ ENST00000371084.7_1_1:64095120-64095192:+ PGM1 Out-of-Frame Out-of-Frame GN=PGM1 Out-of-Frame E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep3_Slot2-5_1_3521 10mio-rep2 E_20221201_NO30_400nL_HLAc1_1e7_directIP_titration_rep2_Slot2-3_1_3513.11070.1417.2 2 3 1 11.46 5.36 7.23 6.1 65.3 6 1 1 22400.0 Hydrogen 768 (Q) 9 KSLSEYNNF (K) 9 YFHFLNNF K S L|S\E/Y|N|N\F 66.7 6 5 4 3 0 dual b/y bias 38.245457 0.865946 551.2611 1101.521 -0.0064 -5.8 0.9000000000000004 6.0 213870.4 nuORFdb_human ENST00000617998.4_2_17:7388053-7417318:+ ENST00000617998.4_2_17:7388053-7417318:+ POLR2A 5' Overlap uORF 5' Overlap uORF GN=POLR2A 5' Overlap uORF E_20221201_NO30_400nL_HLAc1_1e7_directIP_titration_rep2_Slot2-3_1_3513 10mio-rep3 E_20221201_NO30_400nL_HLAc1_1e7_directIP_titration_rep3_Slot2-3_1_3514.11051.1440.2 2 2 1 7.83 3.67 5.41 4.16 56.1 6 1 1 9040.0 Hydrogen 768 (Q) 9 KSLSEYNNF (K) 9 YFHFLNNF K S L\S\E/Y|N\N\F 66.7 6 5 2 1 0 b-ion bias 38.180363 0.851052 551.2598 1101.521 -0.0084 -7.6 -1.2999999999999998 6.0 213870.4 nuORFdb_human ENST00000617998.4_2_17:7388053-7417318:+ ENST00000617998.4_2_17:7388053-7417318:+ POLR2A 5' Overlap uORF 5' Overlap uORF GN=POLR2A 5' Overlap uORF E_20221201_NO30_400nL_HLAc1_1e7_directIP_titration_rep3_Slot2-3_1_3514 20mio-rep1 E_20221201_NO30_400nL_HLAc1_2e7_directIP_titration_rep1_Slot2-4_1_3515.10992.1423.2 2 1 1 7.5 2.33 5.46 5.16 64.2 6 1 1 14000.0 Hydrogen 768 (Q) 9 KSLSEYNNF (K) 9 YFHFLNNF K S L/S\E/Y\N\N\F 66.7 6 4 2 0 0 b-ion bias 37.966103 0.86177 551.2601 1101.521 -0.0084 -7.6 -1.3999999999999995 6.0 213870.4 nuORFdb_human ENST00000617998.4_2_17:7388053-7417318:+ ENST00000617998.4_2_17:7388053-7417318:+ POLR2A 5' Overlap uORF 5' Overlap uORF GN=POLR2A 5' Overlap uORF E_20221201_NO30_400nL_HLAc1_2e7_directIP_titration_rep1_Slot2-4_1_3515 20mio-rep2 E_20221201_NO30_400nL_HLAc1_2e7_directIP_titration_rep3_Slot2-4_1_3518.11166.1417.2 2 3 1 9.89 5.45 6.64 4.44 69.9 6 1 1 25900.0 Hydrogen 768 (Q) 9 KSLSEYNNF (K) 9 VKTSEFEnF K S L|S\E/Y\N\N\F 66.7 6 5 2 1 0 b-ion bias 38.56908 0.865584 551.2615 1101.521 -0.0044 -4.0 2.3 6.0 213870.4 nuORFdb_human ENST00000617998.4_2_17:7388053-7417318:+ ENST00000617998.4_2_17:7388053-7417318:+ POLR2A 5' Overlap uORF 5' Overlap uORF GN=POLR2A 5' Overlap uORF E_20221201_NO30_400nL_HLAc1_2e7_directIP_titration_rep3_Slot2-4_1_3518 40mio-rep1 E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep1_Slot1-04_1_3496.11312.1373.2 2 1 1 12.54 4.75 8.58 7.8 79.3 6 1 1 26000.0 Hydrogen 768 (Q) 9 KSLSEYNNF (K) 9 YFHFLNNF K S L|S\E/Y|N|N\F 66.7 6 5 4 3 0 dual b/y bias 39.076462 0.892965 551.2631 1101.521 -0.0024 -2.2 1.0 6.0 213870.4 nuORFdb_human ENST00000617998.4_2_17:7388053-7417318:+ ENST00000617998.4_2_17:7388053-7417318:+ POLR2A 5' Overlap uORF 5' Overlap uORF GN=POLR2A 5' Overlap uORF E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep1_Slot1-04_1_3496 40mio-rep2 E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep2_Slot2-5_1_3520.10994.1393.2 2 1 1 9.13 5.14 6.19 3.99 69.6 6 1 1 24800.0 Hydrogen 768 (Q) 9 KSLSEYNNF (K) 9 VKTSEFEnF K S L|S\E/Y\N\N\F 66.7 6 5 2 1 0 b-ion bias 37.97963 0.880691 551.2619 1101.521 -0.0044 -4.0 0.9000000000000004 6.0 213870.4 nuORFdb_human ENST00000617998.4_2_17:7388053-7417318:+ ENST00000617998.4_2_17:7388053-7417318:+ POLR2A 5' Overlap uORF 5' Overlap uORF GN=POLR2A 5' Overlap uORF E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep2_Slot2-5_1_3520 40mio-rep3 E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep3_Slot2-5_1_3521.11001.1396.2 2 2 1 11.47 4.61 6.99 6.87 76.3 6 1 1 69600.0 Hydrogen 768 (Q) 9 KSLSEYNNF (K) 9 YFHFLNNF K S L\S\E/Y\N|N\F 66.7 6 5 2 1 0 b-ion bias 38.006428 0.878673 551.2619 1101.521 -0.0044 -4.0 0.5999999999999996 6.0 213870.4 nuORFdb_human ENST00000617998.4_2_17:7388053-7417318:+ ENST00000617998.4_2_17:7388053-7417318:+ POLR2A 5' Overlap uORF 5' Overlap uORF GN=POLR2A 5' Overlap uORF E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep3_Slot2-5_1_3521 40mio-rep4 E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep4_Slot2-5_1_3522.11005.1396.2 2 1 1 10.71 4.68 6.2 6.03 72.4 6 1 1 16300.0 Hydrogen 768 (Q) 9 KSLSEYNNF (K) 9 YFHFLNNF K S L|S\E/Y\N|N\F 66.7 6 5 3 2 0 unbiased 38.005642 0.878712 551.2622 1101.521 -0.0044 -4.0 0.7000000000000002 6.0 213870.4 nuORFdb_human ENST00000617998.4_2_17:7388053-7417318:+ ENST00000617998.4_2_17:7388053-7417318:+ POLR2A 5' Overlap uORF 5' Overlap uORF GN=POLR2A 5' Overlap uORF E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep4_Slot2-5_1_3522 10mio-rep1 E_20221201_NO30_400nL_HLAc1_1e7_directIP_titration_rep1_Slot2-3_1_3512.18192.1411.2 2 1 1 9.64 1.45 7.11 8.19 73.6 6 1 1 3440.0 Hydrogen 33 (S) 9 KSWVFEESY (-) 9 qVHSASALESY K S W\V|F|E|E|S\Y 66.7 6 6 4 4 0 dual b/y bias 62.842793 0.869163 587.7691 1174.542 -0.0108 -9.2 -1.4999999999999991 4.53 4860.5 nuORFdb_human ENST00000499217.6_1_15:74753929-74754052:+ ENST00000499217.6_1_15:74753929-74754052:+ UBL7-AS1 Antisense lncRNA GN=UBL7-AS1 Antisense E_20221201_NO30_400nL_HLAc1_1e7_directIP_titration_rep1_Slot2-3_1_3512 10mio-rep2 E_20221201_NO30_400nL_HLAc1_1e7_directIP_titration_rep2_Slot2-3_1_3513.18137.1411.2 2 1 1 13.13 5.31 6.82 7.82 80.6 5 1 1 3560.0 Hydrogen 33 (S) 9 KSWVFEESY (-) 9 qVHSASALESY K S W V|F\E|E|S\Y 55.6 5 5 3 3 0 dual b/y bias 62.649559 0.869575 587.7702 1174.542 -0.0088 -7.5 -0.7999999999999998 4.53 4860.5 nuORFdb_human ENST00000499217.6_1_15:74753929-74754052:+ ENST00000499217.6_1_15:74753929-74754052:+ UBL7-AS1 Antisense lncRNA GN=UBL7-AS1 Antisense E_20221201_NO30_400nL_HLAc1_1e7_directIP_titration_rep2_Slot2-3_1_3513 10mio-rep3 E_20221201_NO30_400nL_HLAc1_1e7_directIP_titration_rep3_Slot2-3_1_3514.18089.1410.2 2 2 1 8.7 1.02 8.32 7.68 68.2 6 1 1 5630.0 Hydrogen 33 (S) 9 KSWVFEESY (-) 9 YEEFSKERS K S W\V|F|E\E\S\Y 66.7 6 6 2 2 0 b-ion bias 62.486412 0.870354 587.7691 1174.542 -0.0108 -9.2 -2.8999999999999995 4.53 4860.5 nuORFdb_human ENST00000499217.6_1_15:74753929-74754052:+ ENST00000499217.6_1_15:74753929-74754052:+ UBL7-AS1 Antisense lncRNA GN=UBL7-AS1 Antisense E_20221201_NO30_400nL_HLAc1_1e7_directIP_titration_rep3_Slot2-3_1_3514 20mio-rep1 E_20221201_NO30_400nL_HLAc1_2e7_directIP_titration_rep1_Slot2-4_1_3515.18116.1403.2 2 1 1 13.0 4.13 7.91 8.87 81.0 6 1 1 7200.0 Hydrogen 33 (S) 9 KSWVFEESY (-) 9 qVHSASALESY K S W\V|F|E|E|S\Y 66.7 6 6 4 4 0 dual b/y bias 62.565258 0.87438 587.7703 1174.542 -0.0088 -7.5 -1.2999999999999998 4.53 4860.5 nuORFdb_human ENST00000499217.6_1_15:74753929-74754052:+ ENST00000499217.6_1_15:74753929-74754052:+ UBL7-AS1 Antisense lncRNA GN=UBL7-AS1 Antisense E_20221201_NO30_400nL_HLAc1_2e7_directIP_titration_rep1_Slot2-4_1_3515 20mio-rep2 E_20221201_NO30_400nL_HLAc1_2e7_directIP_titration_rep3_Slot2-4_1_3518.9142.1465.2 2 1 1 8.58 0.21 7.65 8.37 70.4 7 1 1 3770.0 Hydrogen 15 (R) 9 KTHVTPVNW (S) 9 VPKSTLHNW K T/H\V|T|P/V|N\W 77.8 7 5 5 3 0 dual b/y bias 31.58119 0.835529 541.2898 1081.579 -0.0062 -5.7 0.5999999999999996 8.76 12365.2 nuORFdb_human T360116_9:73218787-73219111:+ T360116_9:73218787-73219111:+ lincRNA lncRNA GN=nan lincRNA E_20221201_NO30_400nL_HLAc1_2e7_directIP_titration_rep3_Slot2-4_1_3518 40mio-rep1 E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep1_Slot1-04_1_3496.9266.1417.2 2 1 1 13.24 3.76 11.05 9.49 85.3 6 1 1 15600.0 Hydrogen 15 (R) 9 KTHVTPVNW (S) 9 PAPLSPLSGGW K T H|V|T|P\V|N\W 66.7 6 6 4 4 0 dual b/y bias 32.012165 0.865781 541.291 1081.579 -0.0042 -3.9 -0.6999999999999997 8.76 12365.2 nuORFdb_human T360116_9:73218787-73219111:+ T360116_9:73218787-73219111:+ lincRNA lncRNA GN=nan lincRNA E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep1_Slot1-04_1_3496 40mio-rep3 E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep3_Slot2-5_1_3521.8992.1443.2 2 2 1 11.34 3.82 8.94 7.52 84.0 6 1 1 20600.0 Hydrogen 15 (R) 9 KTHVTPVNW (S) 9 VPKSTLHNW K T H/V|T|P\V|N\W 66.7 6 5 4 3 0 dual b/y bias 31.066778 0.84924 541.2905 1081.579 -0.0062 -5.7 -1.1000000000000005 8.76 12365.2 nuORFdb_human T360116_9:73218787-73219111:+ T360116_9:73218787-73219111:+ lincRNA lncRNA GN=nan lincRNA E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep3_Slot2-5_1_3521 40mio-rep4 E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep4_Slot2-5_1_3522.5790.1594.3 3 1 1 7.39 3.0 5.54 4.4 58.4 6 2 3 13400.0 Hydrogen 3 (R) 10 KTLDSSFKRY (V) 10 KEEAGKGSALGTP K/T L D|S|S/F/K/R Y 50.0 5 2 6 2 0 y-ion bias 20.00272 0.753998 415.5568 1244.663 -0.0069 -5.6 -0.8999999999999995 9.7 2450.8 nuORFdb_human ENST00000521847.5_1_17:78391611-78391674:+ ENST00000521847.5_1_17:78391611-78391674:+ ENDOV ncRNA Processed Transcript lncRNA GN=ENDOV ncRNA Processed Transcript E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep4_Slot2-5_1_3522 40mio-rep1 E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep1_Slot1-04_1_3496.6404.1640.3 3 1 1 10.92 10.92 10.92 62.2 7 3 3 60600.0 M23m Hydrogen 21 (T) 9 KTmKELVRF (L) 9 K/T/m/K|E|L/V/R F 77.8 7 2 7 2 0 y-ion bias m:Oxidized methionine 22.130518 0.725088 389.8881 1151.66 15.9889 -5.1 -1.8999999999999995 9.99 4434.2 nuORFdb_human ENST00000346183.7_1_16:68155976-68156087:+ ENST00000346183.7_1_16:68155976-68156087:+ NFATC3 Out-of-Frame Out-of-Frame GN=NFATC3 Out-of-Frame E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep1_Slot1-04_1_3496 40mio-rep3 E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep3_Slot2-5_1_3521.6152.1650.3 3 1 1 9.02 9.02 9.02 61.3 6 3 3 26500.0 M23m Hydrogen 21 (T) 9 KTmKELVRF (L) 9 K/T/m/K|E/L/V R F 66.7 6 1 6 1 0 y-ion bias m:Oxidized methionine 21.25486 0.718518 389.8873 1151.66 15.9859 -7.7 -3.1000000000000005 9.99 4434.2 nuORFdb_human ENST00000346183.7_1_16:68155976-68156087:+ ENST00000346183.7_1_16:68155976-68156087:+ NFATC3 Out-of-Frame Out-of-Frame GN=NFATC3 Out-of-Frame E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep3_Slot2-5_1_3521 40mio-rep4 E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep4_Slot2-5_1_3522.6174.1650.3 3 2 1 10.01 8.87 10.01 63.1 6 3 3 90100.0 M23m Hydrogen 21 (T) 9 KTmKELVRF (L) 9 K/T m/K|E|L/V/R F 55.6 5 2 6 2 0 y-ion bias m:Oxidized methionine 21.328075 0.718578 389.888 1151.66 15.9889 -5.1 -0.39999999999999947 9.99 4434.2 nuORFdb_human ENST00000346183.7_1_16:68155976-68156087:+ ENST00000346183.7_1_16:68155976-68156087:+ NFATC3 Out-of-Frame Out-of-Frame GN=NFATC3 Out-of-Frame E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep4_Slot2-5_1_3522 40mio-rep1 E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep1_Slot1-04_1_3496.14877.1385.2 2 1 1 7.18 2.18 3.92 5.0 56.1 7 2 2 14000.0 Hydrogen 29 (K) 9 KTSFIIRSL (Q) 9 KTFTVRIVT K/T/S/F/I|I/R\S L 77.8 7 2 6 1 0 y-ion bias 51.389748 0.885721 532.8247 1064.646 -0.0035 -3.3 -0.09999999999999964 11.0 8318.8 nuORFdb_human ENST00000561205.1_1_15:38591689-38614595:+ ENST00000561205.1_1_15:38591689-38614595:+ SPRED1 ncRNA Processed Transcript lncRNA GN=SPRED1 ncRNA Processed Transcript E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep1_Slot1-04_1_3496 40mio-rep4 E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep4_Slot2-5_1_3522.6213.1373.2 2 1 1 11.54 5.7 5.7 5.84 68.9 6 1 1 5370.0 M21m Hydrogen 15 (I) 10 KTWHGSmETF (T) 10 FmHQIQnGTF K T W|H|G-S m\E\T\F 50.0 5 5 2 2 1 b-ion bias m:Oxidized methionine 21.462698 0.892983 620.2736 1223.551 15.9894 -4.5 0.20000000000000018 6.75 4001.7 nuORFdb_human ENST00000299468.11_1_20:62891430-62891532:+ ENST00000299468.11_1_20:62891430-62891532:+|ENST00000609372.1_1_20:62891430-62904657:+ PCMTD2 Out-of-Frame Out-of-Frame GN=PCMTD2 Out-of-Frame E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep4_Slot2-5_1_3522 20mio-rep1 E_20221201_NO30_400nL_HLAc1_2e7_directIP_titration_rep1_Slot2-4_1_3515.16641.1281.2 2 1 1 13.09 7.79 3.05 5.3 81.0 6 1 1 2740.0 Hydrogen 447 (H) 11 KTYEELSSITY (-) 11 GTSIVFDMSLTY K T Y-E\E L|S|S|I|T\Y 54.5 6 6 4 4 1 dual b/y bias 57.471127 0.950489 667.3265 1333.652 -0.0055 -4.1 2.1000000000000005 4.53 52755.9 nuORFdb_human ENST00000544227.5_1_8:89081601-89339435:- ENST00000544227.5_1_8:89081601-89339435:- MMP16 ncRNA Processed Transcript lncRNA GN=MMP16 ncRNA Processed Transcript E_20221201_NO30_400nL_HLAc1_2e7_directIP_titration_rep1_Slot2-4_1_3515 20mio-rep2 E_20221201_NO30_400nL_HLAc1_2e7_directIP_titration_rep3_Slot2-4_1_3518.16698.1273.2 2 1 1 14.31 6.98 4.58 7.33 85.9 7 1 1 2260.0 Hydrogen 447 (H) 11 KTYEELSSITY (-) 11 VWSMMRFLTY K T Y E\E/L|S|S\I|T\Y 63.6 7 6 4 3 0 unbiased 57.676315 0.95594 667.3248 1333.652 -0.0095 -7.1 -0.7999999999999998 4.53 52755.9 nuORFdb_human ENST00000544227.5_1_8:89081601-89339435:- ENST00000544227.5_1_8:89081601-89339435:- MMP16 ncRNA Processed Transcript lncRNA GN=MMP16 ncRNA Processed Transcript E_20221201_NO30_400nL_HLAc1_2e7_directIP_titration_rep3_Slot2-4_1_3518 20mio-rep3 E_20221201_NO30_400nL_HLAc1_2e7_directIP_titration_rep4_Slot2-4_1_3519.16687.1273.2 2 2 1 12.24 9.28 1.96 2.96 77.6 5 1 1 4860.0 Hydrogen 447 (H) 11 KTYEELSSITY (-) 11 GTSIVFDMSLTY K T Y-E E L|S|S\I|T\Y 45.5 5 5 3 3 1 unbiased 57.637268 0.955616 667.3266 1333.652 -0.0055 -4.1 1.7000000000000002 4.53 52755.9 nuORFdb_human ENST00000544227.5_1_8:89081601-89339435:- ENST00000544227.5_1_8:89081601-89339435:- MMP16 ncRNA Processed Transcript lncRNA GN=MMP16 ncRNA Processed Transcript E_20221201_NO30_400nL_HLAc1_2e7_directIP_titration_rep4_Slot2-4_1_3519 40mio-rep1 E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep1_Slot1-04_1_3496.16874.1244.2 2 1 1 9.58 5.63 2.85 3.95 73.4 5 1 1 4110.0 Hydrogen 447 (H) 11 KTYEELSSITY (-) 11 VWSMMRFLTY K T Y E E L|S\S\I|T\Y 45.5 5 5 2 2 0 b-ion bias 58.287395 0.973625 667.3299 1333.652 0.0005 0.4 3.6 4.53 52755.9 nuORFdb_human ENST00000544227.5_1_8:89081601-89339435:- ENST00000544227.5_1_8:89081601-89339435:- MMP16 ncRNA Processed Transcript lncRNA GN=MMP16 ncRNA Processed Transcript E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep1_Slot1-04_1_3496 40mio-rep2 E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep2_Slot2-5_1_3520.16589.1275.2 2 1 1 9.67 5.55 2.93 4.12 65.5 6 1 1 4420.0 Hydrogen 447 (H) 11 KTYEELSSITY (-) 11 VWSMMRFLTY K T Y E\E L/S\S|I|T\Y 45.5 5 5 3 2 0 unbiased 57.304325 0.954129 667.3271 1333.652 -0.0055 -4.1 0.8000000000000007 4.53 52755.9 nuORFdb_human ENST00000544227.5_1_8:89081601-89339435:- ENST00000544227.5_1_8:89081601-89339435:- MMP16 ncRNA Processed Transcript lncRNA GN=MMP16 ncRNA Processed Transcript E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep2_Slot2-5_1_3520 40mio-rep3 E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep3_Slot2-5_1_3521.16611.1279.2 2 2 1 7.53 5.33 2.25 2.2 64.2 6 1 1 5600.0 Hydrogen 447 (H) 11 KTYEELSSITY (-) 11 TMKIDnPHITY K T Y E\E/L/S S\I|T\Y 45.5 5 4 3 1 0 unbiased 57.381844 0.951714 667.3257 1333.652 -0.0075 -5.6 -1.0 4.53 52755.9 nuORFdb_human ENST00000544227.5_1_8:89081601-89339435:- ENST00000544227.5_1_8:89081601-89339435:- MMP16 ncRNA Processed Transcript lncRNA GN=MMP16 ncRNA Processed Transcript E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep3_Slot2-5_1_3521 40mio-rep4 E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep4_Slot2-5_1_3522.16586.1274.2 2 2 1 13.65 9.94 2.85 3.71 81.8 6 1 1 6110.0 Hydrogen 447 (H) 11 KTYEELSSITY (-) 11 SNAEPARPDITY K T Y E\E L|S|S\I|T\Y 45.5 5 6 3 3 0 b-ion bias 57.279896 0.955269 667.3236 1333.652 -0.0115 -8.6 -3.8999999999999995 4.53 52755.9 nuORFdb_human ENST00000544227.5_1_8:89081601-89339435:- ENST00000544227.5_1_8:89081601-89339435:- MMP16 ncRNA Processed Transcript lncRNA GN=MMP16 ncRNA Processed Transcript E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep4_Slot2-5_1_3522 10mio-rep1 E_20221201_NO30_400nL_HLAc1_1e7_directIP_titration_rep1_Slot2-3_1_3512.12330.1353.2 2 1 1 9.53 6.71 0.99 2.82 70.7 5 1 1 7260.0 Hydrogen 707 (S) 10 KTYQDIQNTI (K) 10 qGIPQELQQSL K T Y Q D\I|Q|N\T\I 50.0 5 5 2 2 0 b-ion bias 42.597767 0.90582 612.3112 1223.627 -0.0119 -9.7 -1.9999999999999991 5.83 213870.4 nuORFdb_human ENST00000617998.4_2_17:7388053-7417318:+ ENST00000617998.4_2_17:7388053-7417318:+ POLR2A 5' Overlap uORF 5' Overlap uORF GN=POLR2A 5' Overlap uORF E_20221201_NO30_400nL_HLAc1_1e7_directIP_titration_rep1_Slot2-3_1_3512 10mio-rep2 E_20221201_NO30_400nL_HLAc1_1e7_directIP_titration_rep2_Slot2-3_1_3513.12296.1353.2 2 2 1 11.43 6.88 2.75 4.54 73.9 6 1 1 15100.0 Hydrogen 707 (S) 10 KTYQDIQNTI (K) 10 qGIPQELQQSL K T Y Q\D\I|Q|N|T\I 60.0 6 6 3 3 0 b-ion bias 42.480656 0.905553 612.3122 1223.627 -0.0099 -8.1 -1.3999999999999995 5.83 213870.4 nuORFdb_human ENST00000617998.4_2_17:7388053-7417318:+ ENST00000617998.4_2_17:7388053-7417318:+ POLR2A 5' Overlap uORF 5' Overlap uORF GN=POLR2A 5' Overlap uORF E_20221201_NO30_400nL_HLAc1_1e7_directIP_titration_rep2_Slot2-3_1_3513 10mio-rep3 E_20221201_NO30_400nL_HLAc1_1e7_directIP_titration_rep3_Slot2-3_1_3514.12265.1362.2 2 1 1 10.31 5.73 3.95 4.58 64.1 6 1 1 5620.0 Hydrogen 707 (S) 10 KTYQDIQNTI (K) 10 YTIEIQENTL K T Y Q\D\I\Q|N|T\I 60.0 6 6 2 2 0 b-ion bias 42.376755 0.899866 612.3137 1223.627 -0.0059 -4.8 1.5 5.83 213870.4 nuORFdb_human ENST00000617998.4_2_17:7388053-7417318:+ ENST00000617998.4_2_17:7388053-7417318:+ POLR2A 5' Overlap uORF 5' Overlap uORF GN=POLR2A 5' Overlap uORF E_20221201_NO30_400nL_HLAc1_1e7_directIP_titration_rep3_Slot2-3_1_3514 20mio-rep1 E_20221201_NO30_400nL_HLAc1_2e7_directIP_titration_rep1_Slot2-4_1_3515.12216.1353.2 2 1 1 9.53 3.79 4.44 5.74 66.8 7 1 1 8570.0 Hydrogen 707 (S) 10 KTYQDIQNTI (K) 10 TNELSYRDLL K T Y/Q\D\I|Q\N\T\I 70.0 7 6 2 1 0 b-ion bias 42.194897 0.90589 612.314 1223.627 -0.0059 -4.8 1.4000000000000004 5.83 213870.4 nuORFdb_human ENST00000617998.4_2_17:7388053-7417318:+ ENST00000617998.4_2_17:7388053-7417318:+ POLR2A 5' Overlap uORF 5' Overlap uORF GN=POLR2A 5' Overlap uORF E_20221201_NO30_400nL_HLAc1_2e7_directIP_titration_rep1_Slot2-4_1_3515 20mio-rep2 E_20221201_NO30_400nL_HLAc1_2e7_directIP_titration_rep3_Slot2-4_1_3518.12334.1345.2 2 3 1 10.64 6.28 3.34 4.36 73.9 6 1 1 26500.0 Hydrogen 707 (S) 10 KTYQDIQNTI (K) 10 qGIPQELQQSL K T Y Q\D\I\Q|N\T\I 60.0 6 6 1 1 0 b-ion bias 42.603294 0.911051 612.3129 1223.627 -0.0079 -6.5 -0.20000000000000018 5.83 213870.4 nuORFdb_human ENST00000617998.4_2_17:7388053-7417318:+ ENST00000617998.4_2_17:7388053-7417318:+ POLR2A 5' Overlap uORF 5' Overlap uORF GN=POLR2A 5' Overlap uORF E_20221201_NO30_400nL_HLAc1_2e7_directIP_titration_rep3_Slot2-4_1_3518 20mio-rep3 E_20221201_NO30_400nL_HLAc1_2e7_directIP_titration_rep4_Slot2-4_1_3519.12246.1342.2 2 2 1 14.24 8.35 5.8 5.89 83.6 7 1 1 12600.0 Hydrogen 707 (S) 10 KTYQDIQNTI (K) 10 qGIPQELQQSL K T Y\Q|D\I|Q|N\T\I 70.0 7 7 3 3 0 b-ion bias 42.302631 0.912409 612.313 1223.627 -0.0079 -6.5 -0.7000000000000002 5.83 213870.4 nuORFdb_human ENST00000617998.4_2_17:7388053-7417318:+ ENST00000617998.4_2_17:7388053-7417318:+ POLR2A 5' Overlap uORF 5' Overlap uORF GN=POLR2A 5' Overlap uORF E_20221201_NO30_400nL_HLAc1_2e7_directIP_titration_rep4_Slot2-4_1_3519 40mio-rep1 E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep1_Slot1-04_1_3496.12539.1307.2 2 2 1 19.0 10.64 7.55 8.36 89.0 7 1 1 60000.0 Hydrogen 707 (S) 10 KTYQDIQNTI (K) 10 qGIPQELQQSL K T Y|Q|D\I|Q|N|T\I 70.0 7 7 5 5 0 dual b/y bias 43.314438 0.934715 612.3152 1223.627 -0.0039 -3.2 0.0 5.83 213870.4 nuORFdb_human ENST00000617998.4_2_17:7388053-7417318:+ ENST00000617998.4_2_17:7388053-7417318:+ POLR2A 5' Overlap uORF 5' Overlap uORF GN=POLR2A 5' Overlap uORF E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep1_Slot1-04_1_3496 40mio-rep2 E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep2_Slot2-5_1_3520.12204.1325.2 2 1 1 15.42 9.53 4.06 5.89 81.2 7 1 1 26500.0 Hydrogen 707 (S) 10 KTYQDIQNTI (K) 10 qGIPQELQQSL K T Y\Q/D\I|Q|N|T\I 70.0 7 6 4 3 0 unbiased 42.159981 0.922944 612.3137 1223.627 -0.0059 -4.8 0.10000000000000053 5.83 213870.4 nuORFdb_human ENST00000617998.4_2_17:7388053-7417318:+ ENST00000617998.4_2_17:7388053-7417318:+ POLR2A 5' Overlap uORF 5' Overlap uORF GN=POLR2A 5' Overlap uORF E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep2_Slot2-5_1_3520 40mio-rep3 E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep3_Slot2-5_1_3521.12219.1339.2 2 2 1 11.89 7.76 3.55 4.13 69.4 6 1 1 22600.0 Hydrogen 707 (S) 10 KTYQDIQNTI (K) 10 YKVEInENTL K T Y Q\D\I\Q|N\T\I 60.0 6 6 1 1 0 b-ion bias 42.212124 0.914542 612.3145 1223.627 -0.0039 -3.2 1.3999999999999995 5.83 213870.4 nuORFdb_human ENST00000617998.4_2_17:7388053-7417318:+ ENST00000617998.4_2_17:7388053-7417318:+ POLR2A 5' Overlap uORF 5' Overlap uORF GN=POLR2A 5' Overlap uORF E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep3_Slot2-5_1_3521 40mio-rep4 E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep4_Slot2-5_1_3522.12204.1326.2 2 2 1 12.67 8.26 2.57 4.4 77.3 6 1 1 26500.0 Hydrogen 707 (S) 10 KTYQDIQNTI (K) 10 YKVEInENTL K T Y Q\D\I|Q|N\T\I 60.0 6 6 2 2 0 b-ion bias 42.145374 0.922865 612.3132 1223.627 -0.0079 -6.5 -1.7999999999999998 5.83 213870.4 nuORFdb_human ENST00000617998.4_2_17:7388053-7417318:+ ENST00000617998.4_2_17:7388053-7417318:+ POLR2A 5' Overlap uORF 5' Overlap uORF GN=POLR2A 5' Overlap uORF E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep4_Slot2-5_1_3522 5mio-rep1 E_20221201_NO30_400nL_HLAc1_5e6_directIP_titration_rep1_Slot1-37_1_3502.12580.1342.2 2 1 1 10.24 7.54 0.89 2.7 73.2 5 1 1 5480.0 Hydrogen 707 (S) 10 KTYQDIQNTI (K) 10 qGIPQELQQSL K T Y Q D|I|Q\N\T\I 50.0 5 5 2 2 0 b-ion bias 43.455276 0.91274 612.3121 1223.627 -0.0099 -8.1 -1.0999999999999996 5.83 213870.4 nuORFdb_human ENST00000617998.4_2_17:7388053-7417318:+ ENST00000617998.4_2_17:7388053-7417318:+ POLR2A 5' Overlap uORF 5' Overlap uORF GN=POLR2A 5' Overlap uORF E_20221201_NO30_400nL_HLAc1_5e6_directIP_titration_rep1_Slot1-37_1_3502 5mio-rep2 E_20221201_NO30_400nL_HLAc1_5e6_directIP_titration_rep2_Slot2-2_1_3510.12506.1360.2 2 1 1 9.56 7.22 0.82 2.34 72.8 5 1 1 2520.0 Hydrogen 707 (S) 10 KTYQDIQNTI (K) 10 YTIEIQENTL K T Y Q D\I|Q|N\T\I 50.0 5 5 2 2 0 b-ion bias 43.19529 0.901223 612.3106 1223.627 -0.0119 -9.7 -1.0 5.83 213870.4 nuORFdb_human ENST00000617998.4_2_17:7388053-7417318:+ ENST00000617998.4_2_17:7388053-7417318:+ POLR2A 5' Overlap uORF 5' Overlap uORF GN=POLR2A 5' Overlap uORF E_20221201_NO30_400nL_HLAc1_5e6_directIP_titration_rep2_Slot2-2_1_3510 5mio-rep3 E_20221201_NO30_400nL_HLAc1_5e6_directIP_titration_rep3_Slot2-2_1_3511.12527.1361.2 2 1 1 9.13 6.17 2.67 2.96 63.0 6 1 1 2720.0 Hydrogen 707 (S) 10 KTYQDIQNTI (K) 10 YTIEIQENTL K T Y Q/D\I\Q\N\T\I 60.0 6 5 1 0 0 b-ion bias 43.27544 0.900621 612.313 1223.627 -0.0079 -6.5 1.6999999999999993 5.83 213870.4 nuORFdb_human ENST00000617998.4_2_17:7388053-7417318:+ ENST00000617998.4_2_17:7388053-7417318:+ POLR2A 5' Overlap uORF 5' Overlap uORF GN=POLR2A 5' Overlap uORF E_20221201_NO30_400nL_HLAc1_5e6_directIP_titration_rep3_Slot2-2_1_3511 40mio-rep1 E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep1_Slot1-04_1_3496.10344.1303.2 2 2 1 7.31 7.31 7.31 53.7 7 3 3 18900.0 Hydrogen 73 (L) 10 KVKVISMEKF (-) 10 K V/K/V|I\S M\E|K\F 60.0 6 5 4 2 0 unbiased 35.734131 0.936853 604.8543 1208.707 -0.0064 -5.3 -2.0999999999999996 9.7 9551.6 smORF_v4 ZNF788.1_19:12203283-12222119:+ ZNF788.1_19:12203283-12222119:+ ZNF788 lncRNA 19:12203283-12222119:+ 19 lincRNA GN=ZNF788 E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep1_Slot1-04_1_3496 40mio-rep1 E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep1_Slot1-04_1_3496.6483.1303.2 2 2 1 12.04 2.36 9.68 9.68 64.4 7 3 3 10400.0 M79m Hydrogen 73 (L) 10 KVKVISmEKF (-) 10 KRmLKCLIF K/V|K/V|I|S m E\K\F 70.0 7 5 5 3 0 unbiased m:Oxidized methionine 22.403179 0.936788 612.8539 1208.707 15.9936 -1.1 2.1 9.7 9551.6 smORF_v4 ZNF788.1_19:12203283-12222119:+ ZNF788.1_19:12203283-12222119:+ ZNF788 lncRNA 19:12203283-12222119:+ 19 lincRNA GN=ZNF788 E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep1_Slot1-04_1_3496 40mio-rep2 E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep2_Slot2-5_1_3520.6220.1316.2 2 3 1 7.99 7.99 7.99 54.1 7 3 3 73400.0 M79m Hydrogen 73 (L) 10 KVKVISmEKF (-) 10 K/V/K/V|I/S/m E K\F 70.0 7 2 6 1 0 y-ion bias m:Oxidized methionine 21.494654 0.928674 612.8536 1208.707 15.9936 -1.1 3.8000000000000003 9.7 9551.6 smORF_v4 ZNF788.1_19:12203283-12222119:+ ZNF788.1_19:12203283-12222119:+ ZNF788 lncRNA 19:12203283-12222119:+ 19 lincRNA GN=ZNF788 E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep2_Slot2-5_1_3520 40mio-rep3 E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep3_Slot2-5_1_3521.6243.1319.2 2 1 1 8.8 8.8 8.8 52.1 7 3 3 3870.0 M79m Hydrogen 73 (L) 10 KVKVISmEKF (-) 10 K V/K\V|I|S m\E|K\F 60.0 6 6 4 3 0 unbiased m:Oxidized methionine 21.570139 0.926915 612.8524 1208.707 15.9896 -4.4 0.1999999999999993 9.7 9551.6 smORF_v4 ZNF788.1_19:12203283-12222119:+ ZNF788.1_19:12203283-12222119:+ ZNF788 lncRNA 19:12203283-12222119:+ 19 lincRNA GN=ZNF788 E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep3_Slot2-5_1_3521 20mio-rep1 E_20221201_NO30_400nL_HLAc1_2e7_directIP_titration_rep1_Slot2-4_1_3515.21000.1485.2 2 1 1 9.46 6.64 2.1 2.82 78.6 6 1 1 4460.0 Hydrogen 29 (I) 9 KVLDGAIVW (M) 9 LGKnGLLVW K V L\D\G\A\I|V\W 66.7 6 6 1 1 0 b-ion bias 72.520737 0.823177 500.7921 1000.583 -0.0059 -5.9 0.2999999999999998 5.84 5466.4 nuORFdb_human ENST00000496060.1_1_2:95821258-95821402:- ENST00000496060.1_1_2:95821258-95821402:- ZNF514 ncRNA Retained Intron lncRNA GN=ZNF514 ncRNA Retained Intron E_20221201_NO30_400nL_HLAc1_2e7_directIP_titration_rep1_Slot2-4_1_3515 20mio-rep2 E_20221201_NO30_400nL_HLAc1_2e7_directIP_titration_rep3_Slot2-4_1_3518.21036.1478.2 2 1 1 9.67 6.94 2.71 2.73 84.4 5 1 1 6400.0 Hydrogen 29 (I) 9 KVLDGAIVW (M) 9 PRSSRLVW K V L|D\G A\I|V\W 44.4 4 5 2 2 0 b-ion bias 72.662003 0.827586 500.791 1000.583 -0.0079 -7.9 -1.6000000000000005 5.84 5466.4 nuORFdb_human ENST00000496060.1_1_2:95821258-95821402:- ENST00000496060.1_1_2:95821258-95821402:- ZNF514 ncRNA Retained Intron lncRNA GN=ZNF514 ncRNA Retained Intron E_20221201_NO30_400nL_HLAc1_2e7_directIP_titration_rep3_Slot2-4_1_3518 20mio-rep3 E_20221201_NO30_400nL_HLAc1_2e7_directIP_titration_rep4_Slot2-4_1_3519.21070.1473.2 2 1 1 11.81 9.33 3.32 2.48 86.8 5 1 1 4630.0 Hydrogen 29 (I) 9 KVLDGAIVW (M) 9 VQLIDKVW K V L/D\G A\I|V|W 44.4 4 4 3 2 0 unbiased 72.771957 0.830475 500.7908 1000.583 -0.0079 -7.9 -2.1000000000000005 5.84 5466.4 nuORFdb_human ENST00000496060.1_1_2:95821258-95821402:- ENST00000496060.1_1_2:95821258-95821402:- ZNF514 ncRNA Retained Intron lncRNA GN=ZNF514 ncRNA Retained Intron E_20221201_NO30_400nL_HLAc1_2e7_directIP_titration_rep4_Slot2-4_1_3519 40mio-rep2 E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep2_Slot2-5_1_3520.21116.1474.2 2 1 1 11.73 8.5 3.45 3.23 88.6 5 1 1 7260.0 Hydrogen 29 (I) 9 KVLDGAIVW (M) 9 AVDGIIKVW K V L|D G\A\I|V\W 44.4 4 5 2 2 0 b-ion bias 72.94783 0.829581 500.7914 1000.583 -0.0079 -7.9 -3.0 5.84 5466.4 nuORFdb_human ENST00000496060.1_1_2:95821258-95821402:- ENST00000496060.1_1_2:95821258-95821402:- ZNF514 ncRNA Retained Intron lncRNA GN=ZNF514 ncRNA Retained Intron E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep2_Slot2-5_1_3520 40mio-rep3 E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep3_Slot2-5_1_3521.21097.1473.2 2 1 1 9.56 6.41 2.51 3.15 83.1 5 1 1 6340.0 Hydrogen 29 (I) 9 KVLDGAIVW (M) 9 FGVLQLLGGP K V L D|G\A\I|V\W 55.6 5 5 2 2 0 b-ion bias 72.878731 0.830423 500.793 1000.583 -0.0039 -3.9 0.6999999999999997 5.84 5466.4 nuORFdb_human ENST00000496060.1_1_2:95821258-95821402:- ENST00000496060.1_1_2:95821258-95821402:- ZNF514 ncRNA Retained Intron lncRNA GN=ZNF514 ncRNA Retained Intron E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep3_Slot2-5_1_3521 10mio-rep1 E_20221201_NO30_400nL_HLAc1_1e7_directIP_titration_rep1_Slot2-3_1_3512.17314.1334.2 2 3 1 13.95 6.87 7.1 7.07 78.3 7 1 1 11900.0 M13m Hydrogen 5 (F) 10 KVLSSQDImW (A) 10 LVKNLTSnmW K V L/S|S/Q|D|I|m\W 70.0 7 5 6 4 0 dual b/y bias m:Oxidized methionine 59.808815 0.917815 611.8055 1206.619 15.984 -8.9 -1.2000000000000002 5.84 7228.2 smORF_v4|nuORFdb_human RNF216P1_7:5024623-5036354:+ RNF216P1_7:5024623-5036354:+|ENST00000406608.2_1_7:5016050-5028783:+|ENST00000494947.5_1_7:5024574-5024706:+|ENST00000493407.5_1_7:5024574-5026202:+|ENST00000404006.5_1_7:5024574-5028783:+|ENST00000403969.5_1_7:5024574-5036351:+|ENST00000407184.5_2_7:5024574-5087708:+ RNF216P1 Pseudogene 7:5024623-5036354:+ 7 RNF216P1 Pseudogene Pseudogene GN=RNF216P1 E_20221201_NO30_400nL_HLAc1_1e7_directIP_titration_rep1_Slot2-3_1_3512 10mio-rep2 E_20221201_NO30_400nL_HLAc1_1e7_directIP_titration_rep2_Slot2-3_1_3513.17264.1334.2 2 3 1 12.07 8.09 6.79 3.98 75.3 6 1 1 16600.0 M13m Hydrogen 5 (F) 10 KVLSSQDImW (A) 10 LVKnLTSNmW K V L|S\S Q|D\I|m\W 50.0 5 6 3 3 0 b-ion bias m:Oxidized methionine 59.635578 0.917431 611.8049 1206.619 15.984 -8.9 -2.2 5.84 7228.2 smORF_v4|nuORFdb_human RNF216P1_7:5024623-5036354:+ RNF216P1_7:5024623-5036354:+|ENST00000406608.2_1_7:5016050-5028783:+|ENST00000494947.5_1_7:5024574-5024706:+|ENST00000493407.5_1_7:5024574-5026202:+|ENST00000404006.5_1_7:5024574-5028783:+|ENST00000403969.5_1_7:5024574-5036351:+|ENST00000407184.5_2_7:5024574-5087708:+ RNF216P1 Pseudogene 7:5024623-5036354:+ 7 RNF216P1 Pseudogene Pseudogene GN=RNF216P1 E_20221201_NO30_400nL_HLAc1_1e7_directIP_titration_rep2_Slot2-3_1_3513 10mio-rep3 E_20221201_NO30_400nL_HLAc1_1e7_directIP_titration_rep3_Slot2-3_1_3514.17227.1335.2 2 4 1 17.15 9.71 9.29 7.44 83.4 8 1 1 19400.0 M13m Hydrogen 5 (F) 10 KVLSSQDImW (A) 10 LVKNLTSnmW K V/L|S|S|Q\D|I|m\W 80.0 8 7 6 5 0 dual b/y bias m:Oxidized methionine 59.507523 0.917086 611.8063 1206.619 15.986 -7.3 -1.0 5.84 7228.2 smORF_v4|nuORFdb_human RNF216P1_7:5024623-5036354:+ RNF216P1_7:5024623-5036354:+|ENST00000406608.2_1_7:5016050-5028783:+|ENST00000494947.5_1_7:5024574-5024706:+|ENST00000493407.5_1_7:5024574-5026202:+|ENST00000404006.5_1_7:5024574-5028783:+|ENST00000403969.5_1_7:5024574-5036351:+|ENST00000407184.5_2_7:5024574-5087708:+ RNF216P1 Pseudogene 7:5024623-5036354:+ 7 RNF216P1 Pseudogene Pseudogene GN=RNF216P1 E_20221201_NO30_400nL_HLAc1_1e7_directIP_titration_rep3_Slot2-3_1_3514 20mio-rep1 E_20221201_NO30_400nL_HLAc1_2e7_directIP_titration_rep1_Slot2-4_1_3515.17317.1330.2 2 3 1 15.5 10.67 6.14 4.83 84.4 6 1 1 25400.0 M13m Hydrogen 5 (F) 10 KVLSSQDImW (A) 10 LVKNLTSnmW K V L|S/S Q|D|I|m\W 50.0 5 5 5 4 0 dual b/y bias m:Oxidized methionine 59.807011 0.920041 611.807 1206.619 15.988 -5.7 0.5 5.84 7228.2 smORF_v4|nuORFdb_human RNF216P1_7:5024623-5036354:+ RNF216P1_7:5024623-5036354:+|ENST00000406608.2_1_7:5016050-5028783:+|ENST00000494947.5_1_7:5024574-5024706:+|ENST00000493407.5_1_7:5024574-5026202:+|ENST00000404006.5_1_7:5024574-5028783:+|ENST00000403969.5_1_7:5024574-5036351:+|ENST00000407184.5_2_7:5024574-5087708:+ RNF216P1 Pseudogene 7:5024623-5036354:+ 7 RNF216P1 Pseudogene Pseudogene GN=RNF216P1 E_20221201_NO30_400nL_HLAc1_2e7_directIP_titration_rep1_Slot2-4_1_3515 20mio-rep2 E_20221201_NO30_400nL_HLAc1_2e7_directIP_titration_rep3_Slot2-4_1_3518.17318.1328.2 2 3 1 16.51 9.95 7.85 6.56 84.0 7 1 1 18900.0 M13m Hydrogen 5 (F) 10 KVLSSQDImW (A) 10 LVKNLTSnmW K V L|S|S\Q\D|I|m\W 70.0 7 7 4 4 0 dual b/y bias m:Oxidized methionine 59.819038 0.921162 611.8058 1206.619 15.986 -7.3 -1.0 5.84 7228.2 smORF_v4|nuORFdb_human RNF216P1_7:5024623-5036354:+ RNF216P1_7:5024623-5036354:+|ENST00000406608.2_1_7:5016050-5028783:+|ENST00000494947.5_1_7:5024574-5024706:+|ENST00000493407.5_1_7:5024574-5026202:+|ENST00000404006.5_1_7:5024574-5028783:+|ENST00000403969.5_1_7:5024574-5036351:+|ENST00000407184.5_2_7:5024574-5087708:+ RNF216P1 Pseudogene 7:5024623-5036354:+ 7 RNF216P1 Pseudogene Pseudogene GN=RNF216P1 E_20221201_NO30_400nL_HLAc1_2e7_directIP_titration_rep3_Slot2-4_1_3518 20mio-rep3 E_20221201_NO30_400nL_HLAc1_2e7_directIP_titration_rep4_Slot2-4_1_3519.17362.1325.2 2 2 1 17.21 11.48 7.53 5.73 88.3 8 1 1 18300.0 M13m Hydrogen 5 (F) 10 KVLSSQDImW (A) 10 LVKNLTSnmW K V/L|S\S\Q\D|I|m\W 80.0 8 7 4 3 0 unbiased m:Oxidized methionine 59.967354 0.923168 611.8069 1206.619 15.988 -5.7 0.09999999999999964 5.84 7228.2 smORF_v4|nuORFdb_human RNF216P1_7:5024623-5036354:+ RNF216P1_7:5024623-5036354:+|ENST00000406608.2_1_7:5016050-5028783:+|ENST00000494947.5_1_7:5024574-5024706:+|ENST00000493407.5_1_7:5024574-5026202:+|ENST00000404006.5_1_7:5024574-5028783:+|ENST00000403969.5_1_7:5024574-5036351:+|ENST00000407184.5_2_7:5024574-5087708:+ RNF216P1 Pseudogene 7:5024623-5036354:+ 7 RNF216P1 Pseudogene Pseudogene GN=RNF216P1 E_20221201_NO30_400nL_HLAc1_2e7_directIP_titration_rep4_Slot2-4_1_3519 40mio-rep1 E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep1_Slot1-04_1_3496.17471.1287.2 2 5 1 20.5 11.86 9.88 8.64 91.4 8 1 1 50100.0 M13m Hydrogen 5 (F) 10 KVLSSQDImW (A) 10 LVKNLTSnmW K V/L|S|S|Q\D|I|m\W 80.0 8 7 6 5 0 dual b/y bias m:Oxidized methionine 60.349155 0.946788 611.8084 1206.619 15.99 -4.0 -0.7999999999999998 5.84 7228.2 smORF_v4|nuORFdb_human RNF216P1_7:5024623-5036354:+ RNF216P1_7:5024623-5036354:+|ENST00000406608.2_1_7:5016050-5028783:+|ENST00000494947.5_1_7:5024574-5024706:+|ENST00000493407.5_1_7:5024574-5026202:+|ENST00000404006.5_1_7:5024574-5028783:+|ENST00000403969.5_1_7:5024574-5036351:+|ENST00000407184.5_2_7:5024574-5087708:+ RNF216P1 Pseudogene 7:5024623-5036354:+ 7 RNF216P1 Pseudogene Pseudogene GN=RNF216P1 E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep1_Slot1-04_1_3496 40mio-rep2 E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep2_Slot2-5_1_3520.17319.1310.2 2 5 1 15.84 9.66 9.83 6.18 75.6 7 1 1 60400.0 M13m Hydrogen 5 (F) 10 KVLSSQDImW (A) 10 LVKNLTSnmW K V L|S|S\Q|D\I|m\W 70.0 7 7 4 4 0 dual b/y bias m:Oxidized methionine 59.828075 0.932796 611.8084 1206.619 15.99 -4.0 0.9000000000000004 5.84 7228.2 smORF_v4|nuORFdb_human RNF216P1_7:5024623-5036354:+ RNF216P1_7:5024623-5036354:+|ENST00000406608.2_1_7:5016050-5028783:+|ENST00000494947.5_1_7:5024574-5024706:+|ENST00000493407.5_1_7:5024574-5026202:+|ENST00000404006.5_1_7:5024574-5028783:+|ENST00000403969.5_1_7:5024574-5036351:+|ENST00000407184.5_2_7:5024574-5087708:+ RNF216P1 Pseudogene 7:5024623-5036354:+ 7 RNF216P1 Pseudogene Pseudogene GN=RNF216P1 E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep2_Slot2-5_1_3520 40mio-rep3 E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep3_Slot2-5_1_3521.17274.1320.2 2 6 1 17.22 12.92 6.57 4.3 88.5 6 1 1 47900.0 M13m Hydrogen 5 (F) 10 KVLSSQDImW (A) 10 LVKNLTSnmW K V L|S/S Q|D|I|m|W 50.0 5 5 6 5 0 dual b/y bias m:Oxidized methionine 59.671379 0.926431 611.8081 1206.619 15.99 -4.0 0.5999999999999996 5.84 7228.2 smORF_v4|nuORFdb_human RNF216P1_7:5024623-5036354:+ RNF216P1_7:5024623-5036354:+|ENST00000406608.2_1_7:5016050-5028783:+|ENST00000494947.5_1_7:5024574-5024706:+|ENST00000493407.5_1_7:5024574-5026202:+|ENST00000404006.5_1_7:5024574-5028783:+|ENST00000403969.5_1_7:5024574-5036351:+|ENST00000407184.5_2_7:5024574-5087708:+ RNF216P1 Pseudogene 7:5024623-5036354:+ 7 RNF216P1 Pseudogene Pseudogene GN=RNF216P1 E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep3_Slot2-5_1_3521 40mio-rep4 E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep4_Slot2-5_1_3522.17405.1311.2 2 6 1 17.91 8.44 10.61 9.48 82.5 7 1 1 46800.0 M13m Hydrogen 5 (F) 10 KVLSSQDImW (A) 10 LVKnLTSNmW K V L|S|S|Q|D|I|m\W 70.0 7 7 6 6 0 dual b/y bias m:Oxidized methionine 60.108196 0.931981 611.8088 1206.619 15.992 -2.4 2.3000000000000003 5.84 7228.2 smORF_v4|nuORFdb_human RNF216P1_7:5024623-5036354:+ RNF216P1_7:5024623-5036354:+|ENST00000406608.2_1_7:5016050-5028783:+|ENST00000494947.5_1_7:5024574-5024706:+|ENST00000493407.5_1_7:5024574-5026202:+|ENST00000404006.5_1_7:5024574-5028783:+|ENST00000403969.5_1_7:5024574-5036351:+|ENST00000407184.5_2_7:5024574-5087708:+ RNF216P1 Pseudogene 7:5024623-5036354:+ 7 RNF216P1 Pseudogene Pseudogene GN=RNF216P1 E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep4_Slot2-5_1_3522 5mio-rep1 E_20221201_NO30_400nL_HLAc1_5e6_directIP_titration_rep1_Slot1-37_1_3502.17544.1328.2 2 1 1 11.57 7.26 3.86 4.31 72.4 6 1 1 2480.0 M13m Hydrogen 5 (F) 10 KVLSSQDImW (A) 10 LVKnLTSNmW K V L/S|S Q\D|I|m\W 50.0 5 5 4 3 0 unbiased m:Oxidized methionine 60.599461 0.921277 611.8052 1206.619 15.984 -8.9 -1.9000000000000004 5.84 7228.2 smORF_v4|nuORFdb_human RNF216P1_7:5024623-5036354:+ RNF216P1_7:5024623-5036354:+|ENST00000406608.2_1_7:5016050-5028783:+|ENST00000494947.5_1_7:5024574-5024706:+|ENST00000493407.5_1_7:5024574-5026202:+|ENST00000404006.5_1_7:5024574-5028783:+|ENST00000403969.5_1_7:5024574-5036351:+|ENST00000407184.5_2_7:5024574-5087708:+ RNF216P1 Pseudogene 7:5024623-5036354:+ 7 RNF216P1 Pseudogene Pseudogene GN=RNF216P1 E_20221201_NO30_400nL_HLAc1_5e6_directIP_titration_rep1_Slot1-37_1_3502 40mio-rep1 E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep1_Slot1-04_1_3496.16978.1410.2 2 1 1 12.48 9.87 2.03 2.62 86.7 6 1 1 9220.0 Hydrogen 14 (S) 9 KVMDVDTLY (-) 9 QLSIMDTIY K V M\D\V\D\T|L\Y 66.7 6 6 1 1 0 b-ion bias 58.646465 0.870063 542.2729 1083.539 -0.0004 -0.3 2.9000000000000004 4.21 2622.0 nuORFdb_human ENST00000326840.10_1_3:98600587-98620007:- ENST00000326840.10_1_3:98600587-98620007:- DCBLD2 Out-of-Frame Out-of-Frame GN=DCBLD2 Out-of-Frame E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep1_Slot1-04_1_3496 40mio-rep3 E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep3_Slot2-5_1_3521.16631.1430.2 2 1 1 13.45 10.81 3.11 2.64 86.5 6 1 1 7200.0 Hydrogen 14 (S) 9 KVMDVDTLY (-) 9 QLSIMDTIY K V M\D\V|D\T|L\Y 66.7 6 6 2 2 0 b-ion bias 57.450882 0.857701 542.2708 1083.539 -0.0044 -4.0 0.5999999999999996 4.21 2622.0 nuORFdb_human ENST00000326840.10_1_3:98600587-98620007:- ENST00000326840.10_1_3:98600587-98620007:- DCBLD2 Out-of-Frame Out-of-Frame GN=DCBLD2 Out-of-Frame E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep3_Slot2-5_1_3521 40mio-rep1 E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep1_Slot1-04_1_3496.10603.1385.2 2 1 1 8.98 6.91 2.11 2.08 70.8 5 2 2 8630.0 Hydrogen 25 (R) 10 KVREAVGAVW (G) 10 NLKRVAEVW K/V R E A V\G\A|V\W 50.0 5 4 2 1 0 b-ion bias 36.628105 0.88593 557.8211 1114.637 -0.002 -1.8 1.4000000000000001 8.75 5189.8 smORF_v4|nuORFdb_human HUWE1_X:53710819-53707139:- HUWE1_X:53710819-53707139:-|ENST00000446750.1_3_X:53707010-53707565:-|ENST00000342160.7_2_X:53707007-53711004:- HUWE1 uORF X:53710819-53707139:- X HUWE1 5' uORF 5' uORF GN=HUWE1 E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep1_Slot1-04_1_3496 40mio-rep1 E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep1_Slot1-04_1_3496.11605.1430.2 2 2 1 7.37 4.05 3.42 3.32 76.3 5 1 1 16900.0 Hydrogen 43 (E) 9 KVYADTGLY (-) 9 AAVYKDSIY K V/Y|A D\T G\L\Y 33.3 3 4 2 1 0 b-ion bias 40.088562 0.857478 515.2644 1029.525 -0.0044 -4.3 -1.0999999999999996 5.83 4981.4 nuORFdb_human ENST00000312814.10_1_7:112430370-112430523:- ENST00000312814.10_1_7:112430370-112430523:- TMEM168 5' uORF 5' uORF GN=TMEM168 5' uORF E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep1_Slot1-04_1_3496 40mio-rep2 E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep2_Slot2-5_1_3520.11264.1445.2 2 1 1 12.64 7.91 3.72 4.73 87.2 6 1 1 7230.0 Hydrogen 43 (E) 9 KVYADTGLY (-) 9 FTNLTTGIY K V Y/A|D|T\G|L\Y 66.7 6 5 4 3 0 dual b/y bias 38.911556 0.848186 515.2631 1029.525 -0.0064 -6.2 -1.2999999999999998 5.83 4981.4 nuORFdb_human ENST00000312814.10_1_7:112430370-112430523:- ENST00000312814.10_1_7:112430370-112430523:- TMEM168 5' uORF 5' uORF GN=TMEM168 5' uORF E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep2_Slot2-5_1_3520 40mio-rep3 E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep3_Slot2-5_1_3521.11264.1448.2 2 1 1 11.51 7.86 3.58 3.66 81.1 6 1 1 6230.0 Hydrogen 43 (E) 9 KVYADTGLY (-) 9 SLSKnFGIY K V/Y\A\D T\G|L\Y 55.6 5 5 2 1 0 b-ion bias 38.914143 0.846037 515.2628 1029.525 -0.0064 -6.2 -1.6000000000000005 5.83 4981.4 nuORFdb_human ENST00000312814.10_1_7:112430370-112430523:- ENST00000312814.10_1_7:112430370-112430523:- TMEM168 5' uORF 5' uORF GN=TMEM168 5' uORF E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep3_Slot2-5_1_3521 40mio-rep4 E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep4_Slot2-5_1_3522.11280.1454.2 2 1 1 9.36 5.66 4.21 3.71 77.8 6 1 1 9280.0 Hydrogen 43 (E) 9 KVYADTGLY (-) 9 FTNLTTGIY K V/Y|A D\T\G|L\Y 55.6 5 5 3 2 0 unbiased 38.955154 0.842437 515.2648 1029.525 -0.0024 -2.3 2.4000000000000004 5.83 4981.4 nuORFdb_human ENST00000312814.10_1_7:112430370-112430523:- ENST00000312814.10_1_7:112430370-112430523:- TMEM168 5' uORF 5' uORF GN=TMEM168 5' uORF E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep4_Slot2-5_1_3522 Eva-50mio-3fr AL20210827_EV_HLA_PDAC_PANFRO413_2D_50e6_W632_classI_fxn3.42868.42868.2 2 1 1 13.33 8.89 2.72 4.45 88.2 6 1 1 34800000.0 Hydrogen 17 (R) 9 KWLDSPLAF (A) 9 RRMWPLAF K W L\D\S\P\L|A|F 66.7 6 6 2 2 0 b-ion bias 0.903 93.9 77.7 22.03 0.0 538.7923 1076.578 -0.0002 -0.2 -0.7 5.84 4895.6 nuORFdb_human ENST00000398341.7_1_21:43938514-43945908:+ ENST00000398341.7_1_21:43938514-43945908:+ SLC37A1 5' Overlap uORF 5' Overlap uORF GN=SLC37A1 5' Overlap uORF AL20210827_EV_HLA_PDAC_PANFRO413_2D_50e6_W632_classI_fxn3 SCP E_20221201_NO30_400nL_HLAc1_4e7_PDAC_Slot2-6_1_3524.28338.297.1 1 1 1 14.09 6.81 7.26 7.28 85.9 7 1 1 49900.0 Hydrogen 17 (R) 9 KWLDSPLAF (A) 9 KSGGRGSLPAF K/W/L\D|S|P L\A\F 77.8 7 5 4 2 0 unbiased 97.902573 1.546123 1076.5763 1076.578 0.0025 2.3 4.699999999999999 5.84 4895.6 nuORFdb_human ENST00000398341.7_1_21:43938514-43945908:+ ENST00000398341.7_1_21:43938514-43945908:+ SLC37A1 5' Overlap uORF 5' Overlap uORF GN=SLC37A1 5' Overlap uORF E_20221201_NO30_400nL_HLAc1_4e7_PDAC_Slot2-6_1_3524 SCP E_20221201_NO30_400nL_HLAc1_4e7_PDAC_Slot2-6_1_3524.27147.1261.2 2 1 1 9.26 5.08 3.34 4.18 67.2 7 1 1 4160.0 Hydrogen 27 (Q) 11 KWNSLTNSLQF (-) 11 LQRPDFLSSQF K W N S\L\T\N\S|L|Q\F 63.6 7 7 2 2 0 b-ion bias 93.788765 0.963205 669.3461 1337.685 -0.0001 -0.1 2.3 8.75 4478.3 nuORFdb_human ENST00000585960.5_1_19:37047019-37080547:- ENST00000585960.5_1_19:37047019-37080547:- ZNF529 5' Overlap uORF 5' Overlap uORF GN=ZNF529 5' Overlap uORF E_20221201_NO30_400nL_HLAc1_4e7_PDAC_Slot2-6_1_3524 SCP E_20221201_NO30_400nL_HLAc1_4e7_PDAC_Slot2-6_1_3524.14696.1408.2 2 1 1 10.71 3.74 7.64 6.97 61.0 6 1 1 9590.0 Hydrogen 52 (E) 9 KYAFSENTF (N) 9 KFSQDFSTF K Y A|F|S\E|N|T\F 66.7 6 6 4 4 0 dual b/y bias 50.772148 0.871482 553.7599 1106.515 -0.0026 -2.3 0.10000000000000009 6.0 46705.7 nuORFdb_human ENST00000244070.7_1_20:56821016-56884481:- ENST00000244070.7_1_20:56821016-56884481:-|ENST00000493688.5_1_20:56826891-56884481:-|ENST00000334187.12_1_20:56838516-56861449:- PPP4R1L Pseudogene Pseudogene GN=PPP4R1L Pseudogene E_20221201_NO30_400nL_HLAc1_4e7_PDAC_Slot2-6_1_3524 SCP E_20221201_NO30_400nL_HLAc1_4e7_PDAC_Slot2-6_1_3524.13028.1317.2 2 1 1 15.64 7.58 5.84 8.06 88.6 6 1 1 11200.0 Hydrogen 129 (F) 10 KYAPGSLHYF (A) 10 KFDVINHYF K/Y|A|P G S L|H|Y\F 60.0 6 5 5 4 0 dual b/y bias 45.009197 0.928249 591.8011 1182.594 0.0005 0.4 2.8 8.51 21074.7 nuORFdb_human ENST00000521580.2_1_1:35320720-35321281:- ENST00000521580.2_1_1:35320720-35321281:- SMIM12 3' dORF 3' dORF GN=SMIM12 3' dORF E_20221201_NO30_400nL_HLAc1_4e7_PDAC_Slot2-6_1_3524 Exploris AL20221201_MKP_PDAC_01.22344.22344.2 2 1 1 8.36 8.36 8.36 51.3 7 2 2 9860000.0 Hydrogen 21 (R) 9 KYCKLADCW (-) 9 K Y/C/K-L-A\D|C|W 77.8 7 3 4 2 2 unbiased Carbamidomethylation C:Carbamidomethylation, 0.875 100.0 37.97 100.0 0.0 622.2829 1243.56 -0.0013 -1.1 -1.9000000000000001 8.5 3727.3 nuORFdb_human ENST00000368314.5_1_6:127588039-127601431:+ ENST00000368314.5_1_6:127588039-127601431:+|ENST00000476956.5_1_6:127588209-127601431:+ RNF146 5' uORF 5' uORF GN=RNF146 5' uORF AL20221201_MKP_PDAC_01 SCP E_20221201_NO30_400nL_HLAc1_4e7_PDAC_Slot2-6_1_3524.10143.1298.2 2 1 1 11.81 -0.73 11.81 12.54 68.3 8 2 2 3350.0 Hydrogen 21 (R) 9 KYCKLADCW (-) 9 nKHVQEAACW K/Y|C|K\L|A|D|C\W 100.0 9 7 6 5 0 dual b/y bias Carbamidomethylation C:Carbamidomethylation, 35.042282 0.940088 622.2851 1243.56 0.0029 2.3 4.699999999999999 8.5 3727.3 nuORFdb_human ENST00000368314.5_1_6:127588039-127601431:+ ENST00000368314.5_1_6:127588039-127601431:+|ENST00000476956.5_1_6:127588209-127601431:+ RNF146 5' uORF 5' uORF GN=RNF146 5' uORF E_20221201_NO30_400nL_HLAc1_4e7_PDAC_Slot2-6_1_3524 Exploris_FAIMS VH20221207_MKP_FAIMS2022E_HLAc1_4e7_PDAC.28931.28931.2 2 1 1 18.29 18.29 18.29 80.5 8 2 2 3370000.0 Hydrogen 21 (R) 9 KYCKLADCW (-) 9 K/Y/C|K\L\A\D|C|W 100.0 9 6 5 3 0 dual b/y bias Carbamidomethylation C:Carbamidomethylation, 0.736 88.8 57.12 120.0 -50.0 622.2824 1243.56 -0.0023 -1.8 0.8 8.5 3727.3 nuORFdb_human ENST00000368314.5_1_6:127588039-127601431:+ ENST00000368314.5_1_6:127588039-127601431:+|ENST00000476956.5_1_6:127588209-127601431:+ RNF146 5' uORF 5' uORF GN=RNF146 5' uORF VH20221207_MKP_FAIMS2022E_HLAc1_4e7_PDAC Eva-50mio-3fr AL20210827_EV_HLA_PDAC_PANFRO413_2D_50e6_W632_classI_fxn3.34730.34730.2 2 2 1 10.39 9.82 10.39 71.1 6 1 1 141000000.0 Hydrogen 2 (M) 9 KYGVTFYNF (T) 9 K Y G-V\T\F\Y|N/F 66.7 6 4 2 1 1 b-ion bias 0.996 83.4 63.53 24.64 0.0 569.7825 1138.557 0.001 0.9 0.4 8.5 2616.0 nuORFdb_human ENST00000510737.1_1_4:120160395-120160458:+ ENST00000510737.1_1_4:120160395-120160458:+ USP53 ncRNA Processed Transcript lncRNA GN=USP53 ncRNA Processed Transcript AL20210827_EV_HLA_PDAC_PANFRO413_2D_50e6_W632_classI_fxn3 SCP E_20221201_NO30_400nL_HLAc1_4e7_PDAC_Slot2-6_1_3524.11785.1272.2 2 1 1 13.8 4.32 13.8 9.47 80.0 7 2 2 10300.0 Hydrogen 31 (W) 9 KYIKTPYCI (R) 9 KTYLKmLCL K/Y|I|K|T|P Y\C\I 77.8 7 6 5 4 0 dual b/y bias Carbamidomethylation C:Carbamidomethylation, 40.713928 0.956451 593.3178 1185.634 -0.0049 -4.1 -1.6999999999999997 9.53 7928.9 nuORFdb_human ENST00000537956.1_1_12:133563399-133582688:+ ENST00000537956.1_1_12:133563399-133582688:+ ZNF26 ncRNA Processed Transcript lncRNA GN=ZNF26 ncRNA Processed Transcript E_20221201_NO30_400nL_HLAc1_4e7_PDAC_Slot2-6_1_3524 Eva-50mio-3fr AL20210827_EV_HLA_PDAC_PANFRO413_2D_50e6_W632_classI_fxn1.28491.28491.2 2 1 1 11.64 8.62 2.85 3.01 80.1 6 1 1 10700000.0 Hydrogen 9 (M) 9 KYLDNSEIF (I) 9 KSNDLYELF K Y L\D\N\S\E|I|F 66.7 6 6 2 2 0 b-ion bias 0.752 97.1 51.55 48.33 0.0 564.7834 1128.557 0.0025 2.2 2.1 4.37 3292.8 nuORFdb_human ENST00000503671.2_1_4:110739196-110740187:+ ENST00000503671.2_1_4:110739196-110740187:+ GAR1 ncRNA Retained Intron lncRNA GN=GAR1 ncRNA Retained Intron AL20210827_EV_HLA_PDAC_PANFRO413_2D_50e6_W632_classI_fxn1 SCP E_20221201_NO30_400nL_HLAc1_4e7_PDAC_Slot2-6_1_3524.12070.317.1 1 2 1 7.41 2.43 4.73 4.98 65.5 6 1 1 15000.0 Hydrogen 88 (G) 9 KYLPESNTL (Y) 9 ATTLSnAVSSL K Y/L/P-E|S\N T\L 55.6 5 3 3 1 1 unbiased 41.697895 1.534518 1064.5571 1064.562 -0.0023 -2.1 0.2999999999999998 6.0 12038.3 nuORFdb_human ENST00000324677.11_1_17:28011643-28088245:- ENST00000324677.11_1_17:28011643-28088245:-|ENST00000269033.7_1_17:28011643-28257046:-|ENST00000592397.1_1_17:28011643-28166047:- SSH2 ncRNA Processed Transcript lncRNA GN=SSH2 ncRNA Processed Transcript E_20221201_NO30_400nL_HLAc1_4e7_PDAC_Slot2-6_1_3524 SCP E_20221201_NO30_400nL_HLAc1_4e7_PDAC_Slot2-6_1_3524.26490.1277.2 2 5 1 19.57 14.32 9.38 5.25 94.1 8 3 3 126000.0 Hydrogen 4 (A) 9 KYMKEKLLF (V) 9 KYMTAVVKLF K/Y|M|K|E|K\L|L\F 100.0 9 7 6 5 0 dual b/y bias 91.514107 0.953349 600.3461 1199.686 -0.001 -0.8 1.5999999999999999 9.53 2454.9 smORF_v4|nuORFdb_human FASTKD1_2:170428583-170428645:- FASTKD1_2:170428583-170428645:-|ENST00000445210.1_1_2:170428583-170429512:-|ENST00000453153.6_2_2:170428583-170430242:- FASTKD1 uORF 2:170428583-170428645:- 2 FASTKD1 5' uORF 5' uORF GN=FASTKD1 E_20221201_NO30_400nL_HLAc1_4e7_PDAC_Slot2-6_1_3524 SCP E_20221201_NO30_400nL_HLAc1_4e7_PDAC_Slot2-6_1_3524.8738.1272.2 2 2 1 13.34 10.22 6.34 3.13 75.4 6 3 3 68800.0 M6m Hydrogen 4 (A) 9 KYmKEKLLF (V) 9 KYmTAVVKLF K/Y/m K|E K\L\L\F 55.6 5 4 3 1 0 unbiased m:Oxidized methionine 30.187447 0.956393 608.3425 1199.686 15.993 -1.5 0.8999999999999999 9.53 2454.9 smORF_v4|nuORFdb_human FASTKD1_2:170428583-170428645:- FASTKD1_2:170428583-170428645:-|ENST00000445210.1_1_2:170428583-170429512:-|ENST00000453153.6_2_2:170428583-170430242:- FASTKD1 uORF 2:170428583-170428645:- 2 FASTKD1 5' uORF 5' uORF GN=FASTKD1 E_20221201_NO30_400nL_HLAc1_4e7_PDAC_Slot2-6_1_3524 SCP E_20221201_NO30_400nL_HLAc1_4e7_PDAC_Slot2-6_1_3524.27942.1206.2 2 1 1 12.32 3.37 7.17 8.95 71.1 9 2 2 32500.0 Hydrogen 113 (D) 11 KYMKVLFGTEF (N) 11 LNFHVSVLGETF K Y/M|K\V|L\F|G-T\E\F 81.8 9 7 4 3 1 unbiased 96.534561 0.99722 681.8572 1362.713 -0.0059 -4.3 -1.9 8.5 18719.8 nuORFdb_human ENST00000458236.1_1_6:29706493-29716746:- ENST00000458236.1_1_6:29706493-29716746:- HLA-F-AS1 ncRNA Processed Transcript lncRNA GN=HLA-F-AS1 ncRNA Processed Transcript E_20221201_NO30_400nL_HLAc1_4e7_PDAC_Slot2-6_1_3524 Exploris AL20221201_MKP_PDAC_01.46337.46568.2 2 1 1 13.13 6.54 13.13 6.59 81.5 6 1 1 354000000.0 Hydrogen 3 (L) 9 KYPTIICGF (L) 9 FTQLLLCGF K Y\P T\I\I|C/G/F 55.6 5 4 3 1 0 unbiased Carbamidomethylation C:Carbamidomethylation, 0.976 93.9 71.08 6.84 0.0 549.7863 1098.565 0.0001 0.1 -0.7000000000000001 8.59 3524.4 nuORFdb_human ENST00000628006.2_2_17:46730246-46730333:+ ENST00000628006.2_2_17:46730246-46730333:+ LINC02086 lincRNA lncRNA GN=LINC02086 lincRNA AL20221201_MKP_PDAC_01 SCP E_20221201_NO30_400nL_HLAc1_4e7_PDAC_Slot2-6_1_3524.22362.1392.2 2 5 1 15.34 11.66 11.31 3.68 89.3 6 1 1 45900.0 Hydrogen 3 (L) 9 KYPTIICGF (L) 9 FTQLLLCGF K Y|P T|I|I|C|G\F 55.6 5 6 5 5 0 dual b/y bias Carbamidomethylation C:Carbamidomethylation, 77.255997 0.881501 549.7857 1098.565 -0.0005 -0.5 1.9 8.59 3524.4 nuORFdb_human ENST00000628006.2_2_17:46730246-46730333:+ ENST00000628006.2_2_17:46730246-46730333:+ LINC02086 lincRNA lncRNA GN=LINC02086 lincRNA E_20221201_NO30_400nL_HLAc1_4e7_PDAC_Slot2-6_1_3524 Eva-50mio-3fr AL20210827_EV_HLA_PDAC_PANFRO413_2D_50e6_W632_classI_fxn2.33046.33250.2 2 2 2 10.72 10.72 10.72 82.9 6 1 1 39300000.0 C9c Hydrogen 3 (L) 9 KYPTIIcGF (L) 9 K Y|P T-I\I|c/G/F 55.6 5 3 4 2 1 unbiased c:Cysteinylation 0.985 100.0 60.82 100.0 0.0 580.7783 1098.565 61.9841 1.3 1.0 8.59 3524.4 nuORFdb_human ENST00000628006.2_2_17:46730246-46730333:+ ENST00000628006.2_2_17:46730246-46730333:+ LINC02086 lincRNA lncRNA GN=LINC02086 lincRNA AL20210827_EV_HLA_PDAC_PANFRO413_2D_50e6_W632_classI_fxn2 SCP E_20221201_NO30_400nL_HLAc1_4e7_PDAC_Slot2-6_1_3524.16279.617.1 1 1 1 9.58 3.7 5.32 5.87 76.1 7 0 0 7630.0 Hydrogen 75 (R) 9 LAAPAAESF (P) 9 LAAGnVPAY L/A/A/P A\A\E\S\F 77.8 7 4 3 0 0 unbiased 56.239765 1.355842 876.4421 876.446 -0.0042 -4.7 -2.3000000000000003 4.0 12127.8 nuORFdb_human ENST00000339364.9_1_10:98469376-98469727:- ENST00000339364.9_1_10:98469376-98469727:- PIK3AP1 Out-of-Frame Out-of-Frame GN=PIK3AP1 Out-of-Frame E_20221201_NO30_400nL_HLAc1_4e7_PDAC_Slot2-6_1_3524 40mio-rep4 E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep4_Slot2-5_1_3522.9740.1635.3 3 1 1 7.9 6.55 1.71 1.36 67.3 6 1 1 10200.0 Hydrogen 19 (T) 10 LAELERASIA (P) 10 AIGPKmLVDL L A/E/L/E/R A S\I\A 50.0 5 2 4 0 0 y-ion bias 33.639153 0.728177 358.2044 1072.6 -0.0023 -2.1 2.6 4.53 10388.6 nuORFdb_human ENST00000430792.1_1_21:38444859-38445147:- ENST00000430792.1_1_21:38444859-38445147:- PIGP 5' Overlap uORF 5' Overlap uORF GN=PIGP 5' Overlap uORF E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep4_Slot2-5_1_3522 40mio-rep4 E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep4_Slot2-5_1_3522.9587.1649.3 3 2 1 12.29 9.81 4.75 2.48 78.4 5 1 2 17700.0 Hydrogen 1 (-) 10 LASATLVKLK (G) 10 IKSnLKVKL L/A S A T|L|V|K|L-K 60.0 6 4 5 4 1 dual b/y bias 33.110672 0.718894 348.5633 1043.682 -0.0079 -7.5 -2.8 10.0 4083.8 nuORFdb_human T172304_19:28936523-28936721:+ T172304_19:28936523-28936721:+ lincRNA lncRNA GN=nan lincRNA E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep4_Slot2-5_1_3522 10mio-rep3 E_20221201_NO30_400nL_HLAc1_1e7_directIP_titration_rep3_Slot2-3_1_3514.14663.641.1 1 1 1 7.82 4.77 3.01 3.05 70.2 6 0 0 2230.0 Hydrogen 16 (R) 8 LASLQSEV (-) 8 LSAIQSEV L A/S/L/Q\S\E\V 75.0 6 3 3 0 0 unbiased 50.656017 1.341588 846.4554 846.457 -0.0017 -2.0 4.3 4.0 2480.8 smORF_v4|nuORFdb_human REPIN1_7:150065992-150066792:+ REPIN1_7:150065992-150066792:+|ENST00000397281.6_1_7:150065961-150066789:+|ENST00000487455.1_1_7:150065323-150066789:+ REPIN1 uORF 7:150065992-150066792:+ 7 REPIN1 5' Overlap uORF 5' Overlap uORF GN=REPIN1 E_20221201_NO30_400nL_HLAc1_1e7_directIP_titration_rep3_Slot2-3_1_3514 20mio-rep2 E_20221201_NO30_400nL_HLAc1_2e7_directIP_titration_rep3_Slot2-4_1_3518.14747.633.1 1 1 1 11.56 7.72 3.48 3.84 80.3 6 0 0 3450.0 Hydrogen 16 (R) 8 LASLQSEV (-) 8 LSAIQSEV L A/S|L/Q\S\E\V 75.0 6 4 3 1 0 unbiased 50.937538 1.346589 846.4492 846.457 -0.0077 -9.1 -2.8 4.0 2480.8 smORF_v4|nuORFdb_human REPIN1_7:150065992-150066792:+ REPIN1_7:150065992-150066792:+|ENST00000397281.6_1_7:150065961-150066789:+|ENST00000487455.1_1_7:150065323-150066789:+ REPIN1 uORF 7:150065992-150066792:+ 7 REPIN1 5' Overlap uORF 5' Overlap uORF GN=REPIN1 E_20221201_NO30_400nL_HLAc1_2e7_directIP_titration_rep3_Slot2-4_1_3518 40mio-rep4 E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep4_Slot2-5_1_3522.14615.625.1 1 1 1 10.7 6.24 3.79 4.46 78.3 5 0 0 6240.0 Hydrogen 16 (R) 8 LASLQSEV (-) 8 AVTLQDTV L A S|L/Q\S\E\V 62.5 5 4 2 1 0 b-ion bias 50.474594 1.35103 846.45 846.457 -0.0067 -7.9 -3.2 4.0 2480.8 smORF_v4|nuORFdb_human REPIN1_7:150065992-150066792:+ REPIN1_7:150065992-150066792:+|ENST00000397281.6_1_7:150065961-150066789:+|ENST00000487455.1_1_7:150065323-150066789:+ REPIN1 uORF 7:150065992-150066792:+ 7 REPIN1 5' Overlap uORF 5' Overlap uORF GN=REPIN1 E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep4_Slot2-5_1_3522 20mio-rep3 E_20221201_NO30_400nL_HLAc1_2e7_directIP_titration_rep4_Slot2-4_1_3519.18484.1495.2 2 2 1 11.06 7.1 3.16 3.96 71.4 7 1 1 10900.0 Hydrogen 30 (G) 9 LAVDGTRIL (-) 9 AVInGEKLL L/A/V|D|G/T R\I\L 77.8 7 4 5 2 0 unbiased 63.841091 0.816666 479.2861 957.573 -0.008 -8.4 -2.6000000000000005 5.84 3885.3 nuORFdb_human ENST00000427117.5_1_19:37407323-37407440:+ ENST00000427117.5_1_19:37407323-37407440:+ ZNF568 5' uORF 5' uORF GN=ZNF568 5' uORF E_20221201_NO30_400nL_HLAc1_2e7_directIP_titration_rep4_Slot2-4_1_3519 40mio-rep1 E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep1_Slot1-04_1_3496.18723.1479.2 2 1 1 10.98 8.09 2.69 2.89 79.1 6 1 1 5250.0 Hydrogen 30 (G) 9 LAVDGTRIL (-) 9 VALnRSLAI L/A/V/D|G T R\I\L 66.7 6 3 4 1 0 unbiased 64.678932 0.826601 479.2877 957.573 -0.004 -4.2 -1.0 5.84 3885.3 nuORFdb_human ENST00000427117.5_1_19:37407323-37407440:+ ENST00000427117.5_1_19:37407323-37407440:+ ZNF568 5' uORF 5' uORF GN=ZNF568 5' uORF E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep1_Slot1-04_1_3496 40mio-rep2 E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep2_Slot2-5_1_3520.18355.1484.2 2 1 1 10.04 6.97 1.97 3.07 71.6 7 1 1 4360.0 Hydrogen 30 (G) 9 LAVDGTRIL (-) 9 LAVGIWVSI L/A/V|D/G/T R\I\L 77.8 7 3 5 1 0 unbiased 63.408352 0.823329 479.2882 957.573 -0.004 -4.2 0.7000000000000002 5.84 3885.3 nuORFdb_human ENST00000427117.5_1_19:37407323-37407440:+ ENST00000427117.5_1_19:37407323-37407440:+ ZNF568 5' uORF 5' uORF GN=ZNF568 5' uORF E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep2_Slot2-5_1_3520 40mio-rep4 E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep4_Slot2-5_1_3522.18398.1489.2 2 3 1 11.48 10.3 2.5 1.19 82.2 7 1 1 15800.0 Hydrogen 30 (G) 9 LAVDGTRIL (-) 9 VALnRSLAI L/A/V|D|G/T R\I\L 77.8 7 4 5 2 0 unbiased 63.538372 0.820339 479.2879 957.573 -0.004 -4.2 0.5 5.84 3885.3 nuORFdb_human ENST00000427117.5_1_19:37407323-37407440:+ ENST00000427117.5_1_19:37407323-37407440:+ ZNF568 5' uORF 5' uORF GN=ZNF568 5' uORF E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep4_Slot2-5_1_3522 SCP E_20221201_NO30_400nL_HLAc1_4e7_PDAC_Slot2-6_1_3524.16403.1444.2 2 3 1 10.85 9.55 0.3 1.3 73.1 6 2 2 58800.0 Hydrogen 107 (A) 8 LCKEFSKL (E) 8 ERTGSFSKL L/C/K E\F|S|K\L 75.0 6 4 4 2 0 unbiased Carbamidomethylation C:Carbamidomethylation, 56.667915 0.84896 512.776 1024.55 -0.0049 -4.7 -2.3000000000000003 8.59 22570.7 nuORFdb_human ENST00000504733.1_1_3:130546621-130547209:+ ENST00000504733.1_1_3:130546621-130547209:+ GSTO3P Pseudogene Pseudogene GN=GSTO3P Pseudogene E_20221201_NO30_400nL_HLAc1_4e7_PDAC_Slot2-6_1_3524 40mio-rep1 E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep1_Slot1-04_1_3496.16569.1332.2 2 3 1 12.6 10.64 0.07 1.96 87.8 5 0 1 83900.0 M19m Hydrogen 16 (G) 10 LDLmPLEPSK (M) 10 LDPLLmSEPK L/D/L m|P\L|E P S K 40.0 4 3 4 2 0 unbiased m:Oxidized methionine 57.23431 0.918814 579.8047 1142.613 15.9902 -4.1 -0.8999999999999995 4.37 3534.0 nuORFdb_human ENST00000439362.5_1_2:111965809-111965902:- ENST00000439362.5_1_2:111965809-111965902:- MIR4435-2HG lincRNA lncRNA GN=MIR4435-2HG lincRNA E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep1_Slot1-04_1_3496 20mio-rep3 E_20221201_NO30_400nL_HLAc1_2e7_directIP_titration_rep4_Slot2-4_1_3519.16285.1669.3 3 1 1 13.88 6.63 5.98 7.24 68.4 6 2 2 5300.0 M5m Acetyl 1 (-) 10 LDSKmIIKEY (R) 10 EImDKLKEYL L D S K|m|I|I/K/E\Y 60.0 6 4 5 3 0 unbiased m:Oxidized methionine 56.248901 0.70652 433.2254 1281.676 15.9845 -8.0 -2.2 6.07 51964.6 nuORFdb_human ENST00000451868.2_1_12:123484929-123519149:- ENST00000451868.2_1_12:123484929-123519149:-|ENST00000535289.1_1_12:123518987-123519149:- PITPNM2 5' Overlap uORF 5' Overlap uORF GN=PITPNM2 5' Overlap uORF E_20221201_NO30_400nL_HLAc1_2e7_directIP_titration_rep4_Slot2-4_1_3519 40mio-rep2 E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep2_Slot2-5_1_3520.16207.1286.2 2 1 1 9.53 3.42 6.84 6.11 60.7 5 2 2 13100.0 M5m Acetyl 1 (-) 10 LDSKmIIKEY (R) 10 LGTSTAKEAKEY L D S K/m I|I|K\E\Y 40.0 4 4 3 2 0 unbiased m:Oxidized methionine 55.984505 0.94772 649.335 1281.676 15.9868 -6.2 -1.2999999999999998 6.07 51964.6 nuORFdb_human ENST00000451868.2_1_12:123484929-123519149:- ENST00000451868.2_1_12:123484929-123519149:-|ENST00000535289.1_1_12:123518987-123519149:- PITPNM2 5' Overlap uORF 5' Overlap uORF GN=PITPNM2 5' Overlap uORF E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep2_Slot2-5_1_3520 40mio-rep1 E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep1_Slot1-04_1_3496.26005.411.1 1 1 1 9.41 6.13 3.17 3.28 73.4 6 0 0 3510.0 Hydrogen 48 (A) 9 LEGAFISSW (V) 9 IEmKLSSW L E/G A|F\I\S\S\W 55.6 5 5 2 1 0 b-ion bias 89.835945 1.47856 1009.4959 1009.499 0.0011 1.1 4.300000000000001 4.0 9116.2 nuORFdb_human ENST00000489692.1_2_1:235492468-235492705:+ ENST00000489692.1_2_1:235492468-235492705:+ GGPS1 ncRNA Processed Transcript lncRNA GN=GGPS1 ncRNA Processed Transcript E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep1_Slot1-04_1_3496 20mio-rep3 E_20221201_NO30_400nL_HLAc1_2e7_directIP_titration_rep4_Slot2-4_1_3519.10727.1478.2 2 1 1 14.3 13.2 2.13 1.1 91.3 6 1 1 29200.0 Hydrogen 1 (-) 9 LEGQRLQAV (S) 9 RLQGELQAV L E G/Q\R|L|Q\A\V 66.7 6 5 3 2 0 unbiased 37.057915 0.827388 507.2861 1013.574 -0.0091 -9.0 -3.2 6.0 23901.1 nuORFdb_human ENST00000530363.5_1_16:81161532-81175087:- ENST00000530363.5_1_16:81161532-81175087:-|ENST00000533478.5_1_16:81171088-81180967:- PKD1L2 Out-of-Frame Out-of-Frame GN=PKD1L2 Out-of-Frame E_20221201_NO30_400nL_HLAc1_2e7_directIP_titration_rep4_Slot2-4_1_3519 20mio-rep3 E_20221201_NO30_400nL_HLAc1_2e7_directIP_titration_rep4_Slot2-4_1_3519.24749.409.1 1 1 1 10.3 8.51 1.0 1.79 80.3 5 0 0 3080.0 Hydrogen 44 (Q) 9 LELGSEVEL (R) 9 LSGELEVEL L E L G\S\E\V\E\L 55.6 5 5 0 0 0 b-ion bias 85.473862 1.479798 988.5145 988.52 -0.0047 -4.8 1.0 3.67 7660.7 nuORFdb_human ENST00000435580.1_1_1:160033303-160034783:+ ENST00000435580.1_1_1:160033303-160034783:+ RP11-536C5.2 Antisense lncRNA GN=RP11-536C5.2 Antisense E_20221201_NO30_400nL_HLAc1_2e7_directIP_titration_rep4_Slot2-4_1_3519 SCP E_20221201_NO30_400nL_HLAc1_4e7_PDAC_Slot2-6_1_3524.30264.268.1 1 1 1 11.73 10.29 0.47 1.44 85.1 6 0 0 23900.0 Hydrogen 18 (I) 9 LEVLSLVLQ (T) 9 SLVELLVQL L E V\L\S|L\V|L-Q 66.7 6 5 2 2 1 b-ion bias 104.560074 1.563002 1013.6238 1013.624 -0.0041 -4.1 -1.6999999999999997 4.0 7412.8 nuORFdb_human ENST00000382466.7_2_13:21947725-21947908:- ENST00000382466.7_2_13:21947725-21947908:- ZDHHC20 3' dORF 3' dORF GN=ZDHHC20 3' dORF E_20221201_NO30_400nL_HLAc1_4e7_PDAC_Slot2-6_1_3524 40mio-rep1 E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep1_Slot1-04_1_3496.23755.1320.2 2 1 1 9.55 1.51 8.68 8.04 57.4 7 1 1 6650.0 Hydrogen 19 (S) 9 LFSIRQWTY (R) 9 NKFGSIRQTY L F/S\I/R\Q|W|T\Y 77.8 7 5 4 2 0 unbiased 82.063217 0.926173 607.3194 1213.636 -0.0057 -4.7 -1.5 8.75 4175.5 smORF_v4|nuORFdb_human SNHG8_4:119200567-119200869:+ SNHG8_4:119200567-119200869:+|ENST00000602414.5_1_4:119200566-119200866:+ SNHG8 lncRNA 4:119200567-119200869:+ 4 SNHG8 lincRNA lncRNA GN=SNHG8 E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep1_Slot1-04_1_3496 10mio-rep1 E_20221201_NO30_400nL_HLAc1_1e7_directIP_titration_rep1_Slot2-3_1_3512.22654.720.1 1 1 1 11.37 9.79 0.25 1.58 84.8 6 0 0 5030.0 Hydrogen 64 (S) 9 LGAGAVLGV (G) 9 IQVAGIGV L G/A/G\A V|L\G\V 55.6 5 4 3 1 0 unbiased 78.250511 1.294291 756.455 756.461 -0.0064 -8.5 -0.7999999999999998 5.52 8986.9 nuORFdb_human ENST00000397640.5_1_17:73996391-73996640:+ ENST00000397640.5_1_17:73996391-73996640:+ TEN1 3' dORF 3' dORF GN=TEN1 3' dORF E_20221201_NO30_400nL_HLAc1_1e7_directIP_titration_rep1_Slot2-3_1_3512 Exploris AL20221201_MKP_PDAC_01.11021.11210.2 2 1 1 9.86 8.19 4.03 1.68 73.6 5 1 1 3410000.0 Acetyl 1 (-) 9 LGCRLSAAA (L) 9 RQNSRSAAA L G C R\L\S\A|A\A 55.6 5 5 1 1 0 b-ion bias Carbamidomethylation C:Carbamidomethylation, 0.859 100.0 20.98 100.0 0.0 480.7507 960.493 0.0009 1.0 0.19999999999999996 9.75 7535.6 nuORFdb_human ENST00000453527.6_1_8:11710880-11725540:- ENST00000453527.6_1_8:11710880-11725540:-|ENST00000534636.5_1_8:11710981-11725540:-|ENST00000534510.5_1_8:11710880-11725540:- CTSB 5' Overlap uORF 5' Overlap uORF GN=CTSB 5' Overlap uORF AL20221201_MKP_PDAC_01 Exploris_FAIMS VH20221207_MKP_FAIMS2022E_HLAc1_4e7_PDAC.14949.14949.2 2 1 1 11.66 8.03 4.64 3.63 80.0 5 1 1 2280000.0 Acetyl 1 (-) 9 LGCRLSAAA (L) 9 RGLTWDAAA L G C R\L\S\A|A\A 55.6 5 5 1 1 0 b-ion bias Carbamidomethylation C:Carbamidomethylation, 0.99 93.2 32.42 120.0 -50.0 480.7498 960.493 -0.0009 -0.9 1.7000000000000002 9.75 7535.6 nuORFdb_human ENST00000453527.6_1_8:11710880-11725540:- ENST00000453527.6_1_8:11710880-11725540:-|ENST00000534636.5_1_8:11710981-11725540:-|ENST00000534510.5_1_8:11710880-11725540:- CTSB 5' Overlap uORF 5' Overlap uORF GN=CTSB 5' Overlap uORF VH20221207_MKP_FAIMS2022E_HLAc1_4e7_PDAC 40mio-rep2 E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep2_Slot2-5_1_3520.12838.743.1 1 1 1 10.21 7.4 2.98 2.81 77.6 7 0 0 4000.0 Hydrogen 24 (S) 9 LGPPASVSA (-) 9 LGPLPGSSA L/G/P/P A\S\V\S\A 77.8 7 4 3 0 0 unbiased 44.348919 1.280525 798.429 798.436 -0.0066 -8.3 -3.4000000000000004 5.52 3342.8 smORF_v4|nuORFdb_human AGGF1_5:76326450-76326548:+ AGGF1_5:76326450-76326548:+|ENST00000312916.11_1_5:76326323-76326545:+ AGGF1 uORF 5:76326450-76326548:+ 5 AGGF1 5' Overlap uORF 5' Overlap uORF GN=AGGF1 E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep2_Slot2-5_1_3520 40mio-rep3 E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep3_Slot2-5_1_3521.12838.743.1 1 2 1 10.25 7.96 2.46 2.29 77.1 7 0 0 5420.0 Hydrogen 24 (S) 9 LGPPASVSA (-) 9 LGPLPGSSA L/G/P/P A\S\V\S\A 77.8 7 4 3 0 0 unbiased 44.353306 1.280319 798.4317 798.436 -0.0036 -4.5 0.09999999999999964 5.52 3342.8 smORF_v4|nuORFdb_human AGGF1_5:76326450-76326548:+ AGGF1_5:76326450-76326548:+|ENST00000312916.11_1_5:76326323-76326545:+ AGGF1 uORF 5:76326450-76326548:+ 5 AGGF1 5' Overlap uORF 5' Overlap uORF GN=AGGF1 E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep3_Slot2-5_1_3521 40mio-rep4 E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep4_Slot2-5_1_3522.12837.738.1 1 2 1 11.78 7.86 4.24 3.92 80.5 7 0 0 5540.0 Hydrogen 24 (S) 9 LGPPASVSA (-) 9 LGPLPGSSA L/G/P/P A\S\V\S\A 77.8 7 4 3 0 0 unbiased 44.330276 1.283178 798.434 798.436 -0.0016 -2.0 2.7 5.52 3342.8 smORF_v4|nuORFdb_human AGGF1_5:76326450-76326548:+ AGGF1_5:76326450-76326548:+|ENST00000312916.11_1_5:76326323-76326545:+ AGGF1 uORF 5:76326450-76326548:+ 5 AGGF1 5' Overlap uORF 5' Overlap uORF GN=AGGF1 E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep4_Slot2-5_1_3522 40mio-rep4 E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep4_Slot2-5_1_3522.22936.426.1 1 1 1 7.18 4.89 0.19 2.29 57.3 6 0 0 4320.0 Hydrogen 3 (C) 10 LGVPELVPSA (A) 10 EAALLPLPSA L G V P\E\L\V|P\S\A 60.0 6 6 1 1 0 b-ion bias 79.218788 1.469732 981.5551 981.562 -0.0065 -6.6 -1.8999999999999995 4.0 17281.6 nuORFdb_human ENST00000565665.5_1_16:1383676-1389335:+ ENST00000565665.5_1_16:1383676-1389335:+ BAIAP3 5' Overlap uORF 5' Overlap uORF GN=BAIAP3 5' Overlap uORF E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep4_Slot2-5_1_3522 40mio-rep1 E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep1_Slot1-04_1_3496.27790.422.1 1 1 1 9.71 8.07 2.58 1.64 78.1 6 0 0 8750.0 Hydrogen 20 (I) 9 LISGDLNLS (W) 9 SLLDGLNSL L I S/G\D\L\N\L-S 66.7 6 4 1 0 1 b-ion bias 96.006462 1.472046 931.5065 931.51 -0.0035 -3.7 -0.5 3.8 36445.8 nuORFdb_human ENST00000534119.1_1_11:58715222-58723497:+ ENST00000534119.1_1_11:58715222-58723497:+ GLYATL1 3' Overlap dORF 3' Overlap dORF GN=GLYATL1 3' Overlap dORF E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep1_Slot1-04_1_3496 40mio-rep1 E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep1_Slot1-04_1_3496.26368.90.1 1 2 1 7.19 0.6 5.1 6.59 68.6 5 0 0 2870.0 Hydrogen 67 (W) 9 LLDDYNYFY (F) 9 RGCVFNYFY L L D D\Y/N\Y\F\Y 55.6 5 4 1 0 0 b-ion bias 91.092079 1.667069 1225.5396 1225.541 -0.0012 -1.0 2.2 3.56 8551.7 nuORFdb_human ENST00000303406.4_3_12:54410833-54411064:+ ENST00000303406.4_3_12:54410833-54411064:+ HOXC4 5' uORF 5' uORF GN=HOXC4 5' uORF E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep1_Slot1-04_1_3496 20mio-rep1 E_20221201_NO30_400nL_HLAc1_2e7_directIP_titration_rep1_Slot2-4_1_3515.18226.1504.2 2 1 1 11.34 7.24 4.73 4.1 84.5 8 1 1 6510.0 Hydrogen 7 (W) 9 LLDLPGGKY (I) 9 LInLQNFL L/L/D|L|P/G/G\K\Y 100.0 9 4 6 2 0 unbiased 62.944939 0.810697 488.275 975.551 -0.0082 -8.4 -2.2 5.83 2216.7 nuORFdb_human ENST00000557644.5_1_12:97330441-97330501:+ ENST00000557644.5_1_12:97330441-97330501:+ NEDD1 Out-of-Frame Out-of-Frame GN=NEDD1 Out-of-Frame E_20221201_NO30_400nL_HLAc1_2e7_directIP_titration_rep1_Slot2-4_1_3515 20mio-rep3 E_20221201_NO30_400nL_HLAc1_2e7_directIP_titration_rep4_Slot2-4_1_3519.18267.1504.2 2 1 1 10.59 8.03 3.6 2.56 88.8 7 1 1 4320.0 Hydrogen 7 (W) 9 LLDLPGGKY (I) 9 ILDLIPYE L/L|D|L|P\G/G K\Y 77.8 7 5 5 3 0 dual b/y bias 63.091297 0.810803 488.2754 975.551 -0.0082 -8.4 -2.6000000000000005 5.83 2216.7 nuORFdb_human ENST00000557644.5_1_12:97330441-97330501:+ ENST00000557644.5_1_12:97330441-97330501:+ NEDD1 Out-of-Frame Out-of-Frame GN=NEDD1 Out-of-Frame E_20221201_NO30_400nL_HLAc1_2e7_directIP_titration_rep4_Slot2-4_1_3519 40mio-rep1 E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep1_Slot1-04_1_3496.18456.1476.2 2 1 1 12.75 8.61 4.72 4.14 92.5 6 1 1 7910.0 Hydrogen 7 (W) 9 LLDLPGGKY (I) 9 LInLQNFL L/L|D|L|P|G G K\Y 66.7 6 5 5 4 0 dual b/y bias 63.753429 0.828603 488.2769 975.551 -0.0042 -4.3 -1.0999999999999996 5.83 2216.7 nuORFdb_human ENST00000557644.5_1_12:97330441-97330501:+ ENST00000557644.5_1_12:97330441-97330501:+ NEDD1 Out-of-Frame Out-of-Frame GN=NEDD1 Out-of-Frame E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep1_Slot1-04_1_3496 40mio-rep2 E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep2_Slot2-5_1_3520.18150.1501.2 2 1 1 10.45 7.79 2.65 2.65 84.7 6 1 1 5990.0 Hydrogen 7 (W) 9 LLDLPGGKY (I) 9 IInIKGGYP L/L|D|L|P|G G K\Y 66.7 6 5 5 4 0 dual b/y bias 62.697815 0.813079 488.2764 975.551 -0.0062 -6.4 -1.5 5.83 2216.7 nuORFdb_human ENST00000557644.5_1_12:97330441-97330501:+ ENST00000557644.5_1_12:97330441-97330501:+ NEDD1 Out-of-Frame Out-of-Frame GN=NEDD1 Out-of-Frame E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep2_Slot2-5_1_3520 40mio-rep3 E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep3_Slot2-5_1_3521.18178.1503.2 2 1 1 8.51 6.44 2.08 2.06 84.2 6 1 1 6840.0 Hydrogen 7 (W) 9 LLDLPGGKY (I) 9 ILDLFQLN L/L/D|L|P/G G K\Y 66.7 6 3 5 2 0 unbiased 62.795479 0.811809 488.2767 975.551 -0.0042 -4.3 0.2999999999999998 5.83 2216.7 nuORFdb_human ENST00000557644.5_1_12:97330441-97330501:+ ENST00000557644.5_1_12:97330441-97330501:+ NEDD1 Out-of-Frame Out-of-Frame GN=NEDD1 Out-of-Frame E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep3_Slot2-5_1_3521 40mio-rep4 E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep4_Slot2-5_1_3522.18179.1499.2 2 1 1 10.09 8.91 2.73 1.18 88.8 6 1 1 7770.0 Hydrogen 7 (W) 9 LLDLPGGKY (I) 9 LLDLmKNL L/L|D|L|P G/G K\Y 55.6 5 4 5 3 0 dual b/y bias 62.781258 0.814252 488.2764 975.551 -0.0062 -6.4 -1.7000000000000002 5.83 2216.7 nuORFdb_human ENST00000557644.5_1_12:97330441-97330501:+ ENST00000557644.5_1_12:97330441-97330501:+ NEDD1 Out-of-Frame Out-of-Frame GN=NEDD1 Out-of-Frame E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep4_Slot2-5_1_3522 20mio-rep3 E_20221201_NO30_400nL_HLAc1_2e7_directIP_titration_rep4_Slot2-4_1_3519.23601.374.1 1 1 1 10.1 3.15 5.76 6.95 79.7 6 0 0 2060.0 Hydrogen 50 (V) 9 LLDSTVLAY (R) 9 ALSVSEILY L L D\S/T\V\L\A\Y 66.7 6 5 1 0 0 b-ion bias 81.509804 1.500957 994.5409 994.545 -0.0045 -4.6 1.2000000000000002 3.8 12276.5 nuORFdb_human ENST00000604849.1_2_X:53172048-53172363:+ ENST00000604849.1_2_X:53172048-53172363:+ KANTR ncRNA Processed Transcript lncRNA GN=KANTR ncRNA Processed Transcript E_20221201_NO30_400nL_HLAc1_2e7_directIP_titration_rep4_Slot2-4_1_3519 40mio-rep1 E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep1_Slot1-04_1_3496.23811.364.1 1 2 1 9.65 3.79 5.81 5.85 75.6 6 0 0 7960.0 Hydrogen 50 (V) 9 LLDSTVLAY (R) 9 LSLSDLLYA L L D\S-T\V\L\A\Y 66.7 6 5 0 0 1 b-ion bias 82.256592 1.506827 994.5457 994.545 0.0005 0.5 3.7 3.8 12276.5 nuORFdb_human ENST00000604849.1_2_X:53172048-53172363:+ ENST00000604849.1_2_X:53172048-53172363:+ KANTR ncRNA Processed Transcript lncRNA GN=KANTR ncRNA Processed Transcript E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep1_Slot1-04_1_3496 40mio-rep3 E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep3_Slot2-5_1_3521.23549.379.1 1 1 1 8.7 4.01 4.01 4.69 78.2 5 0 0 2870.0 Hydrogen 50 (V) 9 LLDSTVLAY (R) 9 DSLVTIAIY L L D\S T\V\L\A\Y 44.4 4 5 0 0 0 b-ion bias 81.348175 1.497979 994.5454 994.545 -0.0005 -0.5 4.1 3.8 12276.5 nuORFdb_human ENST00000604849.1_2_X:53172048-53172363:+ ENST00000604849.1_2_X:53172048-53172363:+ KANTR ncRNA Processed Transcript lncRNA GN=KANTR ncRNA Processed Transcript E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep3_Slot2-5_1_3521 20mio-rep1 E_20221201_NO30_400nL_HLAc1_2e7_directIP_titration_rep1_Slot2-4_1_3515.27890.522.1 1 1 1 8.85 5.95 2.88 2.9 68.2 6 0 0 4520.0 Hydrogen 35 (L) 9 LLFSGAVAL (I) 9 FQSLLVAI L L/F S\G\A\V\A\L 55.6 5 5 1 0 0 b-ion bias 96.307327 1.412582 890.5302 890.535 -0.0046 -5.1 1.1000000000000005 5.52 8017.1 nuORFdb_human ENST00000414046.2_1_6:31430911-31431209:+ ENST00000414046.2_1_6:31430911-31431209:+|ENST00000467369.1_1_6:31430983-31444808:+ HCP5 Sense Overlapping Other GN=HCP5 Sense Overlapping E_20221201_NO30_400nL_HLAc1_2e7_directIP_titration_rep1_Slot2-4_1_3515 20mio-rep2 E_20221201_NO30_400nL_HLAc1_2e7_directIP_titration_rep3_Slot2-4_1_3518.27902.517.1 1 1 1 9.78 6.51 4.1 3.27 72.1 6 0 0 3870.0 Hydrogen 35 (L) 9 LLFSGAVAL (I) 9 FILSQVAL L L/F S\G/A\V\A\L 55.6 5 4 2 0 0 b-ion bias 96.371712 1.415561 890.5272 890.535 -0.0076 -8.5 -2.2 5.52 8017.1 nuORFdb_human ENST00000414046.2_1_6:31430911-31431209:+ ENST00000414046.2_1_6:31430911-31431209:+|ENST00000467369.1_1_6:31430983-31444808:+ HCP5 Sense Overlapping Other GN=HCP5 Sense Overlapping E_20221201_NO30_400nL_HLAc1_2e7_directIP_titration_rep3_Slot2-4_1_3518 SCP E_20221201_NO30_400nL_HLAc1_4e7_PDAC_Slot2-6_1_3524.28646.504.1 1 1 1 10.23 7.4 3.41 2.82 76.0 5 0 0 111000.0 Hydrogen 35 (L) 9 LLFSGAVAL (I) 9 FILSQVAL L L/F|S G A\V\A\L 44.4 4 4 2 1 0 b-ion bias 98.968971 1.42361 890.5334 890.535 -0.0016 -1.8 0.5999999999999999 5.52 8017.1 nuORFdb_human ENST00000414046.2_1_6:31430911-31431209:+ ENST00000414046.2_1_6:31430911-31431209:+|ENST00000467369.1_1_6:31430983-31444808:+ HCP5 Sense Overlapping Other GN=HCP5 Sense Overlapping E_20221201_NO30_400nL_HLAc1_4e7_PDAC_Slot2-6_1_3524 40mio-rep1 E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep1_Slot1-04_1_3496.28020.494.1 1 1 1 10.35 7.24 4.56 3.11 78.9 6 0 0 9570.0 Hydrogen 35 (L) 9 LLFSGAVAL (I) 9 FILSQVAL L L/F/S\G A\V\A\L 55.6 5 4 2 0 0 b-ion bias 96.801567 1.429257 890.534 890.535 -0.0006 -0.7 2.5 5.52 8017.1 nuORFdb_human ENST00000414046.2_1_6:31430911-31431209:+ ENST00000414046.2_1_6:31430911-31431209:+|ENST00000467369.1_1_6:31430983-31444808:+ HCP5 Sense Overlapping Other GN=HCP5 Sense Overlapping E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep1_Slot1-04_1_3496 40mio-rep3 E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep3_Slot2-5_1_3521.27913.511.1 1 1 1 9.72 7.88 2.98 1.84 75.3 5 0 0 7290.0 Hydrogen 35 (L) 9 LLFSGAVAL (I) 9 FQSLLVAI L L/F/S G A\V\A\L 44.4 4 3 2 0 0 unbiased 96.420845 1.419501 890.5342 890.535 -0.0006 -0.7 3.8999999999999995 5.52 8017.1 nuORFdb_human ENST00000414046.2_1_6:31430911-31431209:+ ENST00000414046.2_1_6:31430911-31431209:+|ENST00000467369.1_1_6:31430983-31444808:+ HCP5 Sense Overlapping Other GN=HCP5 Sense Overlapping E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep3_Slot2-5_1_3521 40mio-rep4 E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep4_Slot2-5_1_3522.27916.511.1 1 1 1 9.53 6.6 2.85 2.93 72.4 5 0 0 4720.0 Hydrogen 35 (L) 9 LLFSGAVAL (I) 9 FILSQVAL L L/F|S G A\V\A\L 44.4 4 4 2 1 0 b-ion bias 96.421768 1.419304 890.5334 890.535 -0.0016 -1.8 2.9000000000000004 5.52 8017.1 nuORFdb_human ENST00000414046.2_1_6:31430911-31431209:+ ENST00000414046.2_1_6:31430911-31431209:+|ENST00000467369.1_1_6:31430983-31444808:+ HCP5 Sense Overlapping Other GN=HCP5 Sense Overlapping E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep4_Slot2-5_1_3522 20mio-rep1 E_20221201_NO30_400nL_HLAc1_2e7_directIP_titration_rep1_Slot2-4_1_3515.26351.345.1 1 2 1 15.29 10.29 5.22 5.0 88.8 7 0 0 6250.0 Hydrogen 62 (G) 9 LLIEVSEEL (-) 9 LIELVSLEE L L/I|E|V\S\E\E\L 77.8 7 6 3 2 0 b-ion bias 90.994835 1.518048 1044.5825 1044.582 -0.0023 -2.2 4.0 3.67 8318.8 nuORFdb_human ENST00000561205.1_1_15:38591689-38614595:+ ENST00000561205.1_1_15:38591689-38614595:+ SPRED1 ncRNA Processed Transcript lncRNA GN=SPRED1 ncRNA Processed Transcript E_20221201_NO30_400nL_HLAc1_2e7_directIP_titration_rep1_Slot2-4_1_3515 20mio-rep2 E_20221201_NO30_400nL_HLAc1_2e7_directIP_titration_rep3_Slot2-4_1_3518.26373.337.1 1 2 1 13.02 7.02 5.64 6.0 82.6 6 0 0 5850.0 Hydrogen 62 (G) 9 LLIEVSEEL (-) 9 LLLSEEVEL L L I/E|V\S\E\E\L 66.7 6 5 2 1 0 b-ion bias 91.089981 1.522309 1044.5755 1044.582 -0.0023 -2.2 4.1 3.67 8318.8 nuORFdb_human ENST00000561205.1_1_15:38591689-38614595:+ ENST00000561205.1_1_15:38591689-38614595:+ SPRED1 ncRNA Processed Transcript lncRNA GN=SPRED1 ncRNA Processed Transcript E_20221201_NO30_400nL_HLAc1_2e7_directIP_titration_rep3_Slot2-4_1_3518 20mio-rep3 E_20221201_NO30_400nL_HLAc1_2e7_directIP_titration_rep4_Slot2-4_1_3519.26401.335.1 1 2 1 13.21 8.49 4.95 4.72 80.4 7 0 0 7360.0 Hydrogen 62 (G) 9 LLIEVSEEL (-) 9 LIELVSLEE L L/I|E|V\S\E\E\L 77.8 7 6 3 2 0 b-ion bias 91.182289 1.523505 1044.5774 1044.582 -0.0023 -2.2 3.5999999999999996 3.67 8318.8 nuORFdb_human ENST00000561205.1_1_15:38591689-38614595:+ ENST00000561205.1_1_15:38591689-38614595:+ SPRED1 ncRNA Processed Transcript lncRNA GN=SPRED1 ncRNA Processed Transcript E_20221201_NO30_400nL_HLAc1_2e7_directIP_titration_rep4_Slot2-4_1_3519 40mio-rep1 E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep1_Slot1-04_1_3496.26601.309.1 1 2 1 15.05 9.42 5.91 5.64 87.9 7 0 0 107000.0 Hydrogen 62 (G) 9 LLIEVSEEL (-) 9 LIELVSLEE L L/I|E|V\S\E\E\L 77.8 7 6 3 2 0 b-ion bias 91.89711 1.539115 1044.5785 1044.582 -0.0023 -2.2 1.0 3.67 8318.8 nuORFdb_human ENST00000561205.1_1_15:38591689-38614595:+ ENST00000561205.1_1_15:38591689-38614595:+ SPRED1 ncRNA Processed Transcript lncRNA GN=SPRED1 ncRNA Processed Transcript E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep1_Slot1-04_1_3496 40mio-rep2 E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep2_Slot2-5_1_3520.26369.329.1 1 2 1 15.02 9.1 6.19 5.91 87.4 7 0 0 44000.0 Hydrogen 62 (G) 9 LLIEVSEEL (-) 9 LIELVSLEE L L/I|E|V\S\E\E\L 77.8 7 6 3 2 0 b-ion bias 91.09388 1.527129 1044.578 1044.582 -0.0023 -2.2 2.7 3.67 8318.8 nuORFdb_human ENST00000561205.1_1_15:38591689-38614595:+ ENST00000561205.1_1_15:38591689-38614595:+ SPRED1 ncRNA Processed Transcript lncRNA GN=SPRED1 ncRNA Processed Transcript E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep2_Slot2-5_1_3520 40mio-rep3 E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep3_Slot2-5_1_3521.26368.327.1 1 3 1 12.9 6.52 6.63 6.38 80.8 7 0 0 10400.0 Hydrogen 62 (G) 9 LLIEVSEEL (-) 9 LIELVSLEE L L/I|E|V\S\E\E\L 77.8 7 6 3 2 0 b-ion bias 91.083527 1.528152 1044.5821 1044.582 -0.0023 -2.2 2.3999999999999995 3.67 8318.8 nuORFdb_human ENST00000561205.1_1_15:38591689-38614595:+ ENST00000561205.1_1_15:38591689-38614595:+ SPRED1 ncRNA Processed Transcript lncRNA GN=SPRED1 ncRNA Processed Transcript E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep3_Slot2-5_1_3521 40mio-rep4 E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep4_Slot2-5_1_3522.26373.327.1 1 3 1 15.48 10.21 5.49 5.26 87.0 7 0 0 29800.0 Hydrogen 62 (G) 9 LLIEVSEEL (-) 9 LIELVSLEE L L/I|E|V|S\E\E\L 77.8 7 6 4 3 0 dual b/y bias 91.091072 1.528391 1044.5764 1044.582 -0.0023 -2.2 2.5 3.67 8318.8 nuORFdb_human ENST00000561205.1_1_15:38591689-38614595:+ ENST00000561205.1_1_15:38591689-38614595:+ SPRED1 ncRNA Processed Transcript lncRNA GN=SPRED1 ncRNA Processed Transcript E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep4_Slot2-5_1_3522 10mio-rep1 E_20221201_NO30_400nL_HLAc1_1e7_directIP_titration_rep1_Slot2-3_1_3512.24607.438.1 1 2 1 11.9 5.16 4.76 6.73 84.7 6 0 0 4020.0 Hydrogen 28 (T) 9 LLLAEDSPL (S) 9 LLLnAELSP L L L|A|E\D\S\P\L 66.7 6 6 2 2 0 b-ion bias 84.996368 1.462516 970.538 970.545 -0.0075 -7.8 -0.09999999999999964 3.66 5911.7 smORF_v4|nuORFdb_human IFRD1_7:112090542-112090700:+ IFRD1_7:112090542-112090700:+|ENST00000403825.7_1_7:112090541-112090697:+ IFRD1 uORF 7:112090542-112090700:+ 7 IFRD1 5' uORF 5' uORF GN=IFRD1 E_20221201_NO30_400nL_HLAc1_1e7_directIP_titration_rep1_Slot2-3_1_3512 10mio-rep3 E_20221201_NO30_400nL_HLAc1_1e7_directIP_titration_rep3_Slot2-3_1_3514.24497.441.1 1 1 1 9.77 5.24 3.99 4.53 82.1 5 0 0 2210.0 Hydrogen 28 (T) 9 LLLAEDSPL (S) 9 IVDVLnSPL L L L|A\E\D\S\P-L 66.7 6 5 1 1 1 b-ion bias 84.616821 1.461291 970.5399 970.545 -0.0055 -5.7 0.5999999999999996 3.66 5911.7 smORF_v4|nuORFdb_human IFRD1_7:112090542-112090700:+ IFRD1_7:112090542-112090700:+|ENST00000403825.7_1_7:112090541-112090697:+ IFRD1 uORF 7:112090542-112090700:+ 7 IFRD1 5' uORF 5' uORF GN=IFRD1 E_20221201_NO30_400nL_HLAc1_1e7_directIP_titration_rep3_Slot2-3_1_3514 20mio-rep1 E_20221201_NO30_400nL_HLAc1_2e7_directIP_titration_rep1_Slot2-4_1_3515.24490.433.1 1 1 1 10.23 6.0 2.93 4.23 83.2 6 0 0 6040.0 Hydrogen 28 (T) 9 LLLAEDSPL (S) 9 LLLnAELSP L L L|A\E\D\S\P\L 66.7 6 6 1 1 0 b-ion bias 84.569344 1.465481 970.5388 970.545 -0.0065 -6.7 -0.5 3.66 5911.7 smORF_v4|nuORFdb_human IFRD1_7:112090542-112090700:+ IFRD1_7:112090542-112090700:+|ENST00000403825.7_1_7:112090541-112090697:+ IFRD1 uORF 7:112090542-112090700:+ 7 IFRD1 5' uORF 5' uORF GN=IFRD1 E_20221201_NO30_400nL_HLAc1_2e7_directIP_titration_rep1_Slot2-4_1_3515 20mio-rep2 E_20221201_NO30_400nL_HLAc1_2e7_directIP_titration_rep3_Slot2-4_1_3518.24502.429.1 1 1 1 11.32 6.82 3.7 4.5 82.1 6 0 0 4630.0 Hydrogen 28 (T) 9 LLLAEDSPL (S) 9 IVDVLnSPL L L L|A\E\D|S\P\L 66.7 6 6 2 2 0 b-ion bias 84.629921 1.468173 970.5433 970.545 -0.0025 -2.6 3.6999999999999997 3.66 5911.7 smORF_v4|nuORFdb_human IFRD1_7:112090542-112090700:+ IFRD1_7:112090542-112090700:+|ENST00000403825.7_1_7:112090541-112090697:+ IFRD1 uORF 7:112090542-112090700:+ 7 IFRD1 5' uORF 5' uORF GN=IFRD1 E_20221201_NO30_400nL_HLAc1_2e7_directIP_titration_rep3_Slot2-4_1_3518 20mio-rep3 E_20221201_NO30_400nL_HLAc1_2e7_directIP_titration_rep4_Slot2-4_1_3519.24531.426.1 1 2 1 14.53 7.39 6.01 7.14 86.8 6 0 0 6830.0 Hydrogen 28 (T) 9 LLLAEDSPL (S) 9 IVDVLnSPL L L L|A|E\D|S|P\L 66.7 6 6 4 4 0 dual b/y bias 84.721443 1.470017 970.5408 970.545 -0.0045 -4.7 1.0999999999999996 3.66 5911.7 smORF_v4|nuORFdb_human IFRD1_7:112090542-112090700:+ IFRD1_7:112090542-112090700:+|ENST00000403825.7_1_7:112090541-112090697:+ IFRD1 uORF 7:112090542-112090700:+ 7 IFRD1 5' uORF 5' uORF GN=IFRD1 E_20221201_NO30_400nL_HLAc1_2e7_directIP_titration_rep4_Slot2-4_1_3519 40mio-rep1 E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep1_Slot1-04_1_3496.24732.408.1 1 2 1 10.88 5.7 3.88 5.18 78.1 5 0 0 11300.0 Hydrogen 28 (T) 9 LLLAEDSPL (S) 9 LLLnAELSP L L L|A\E\D\S\P L 44.4 4 5 1 1 0 b-ion bias 85.439995 1.480565 970.54 970.545 -0.0055 -5.7 -2.5 3.66 5911.7 smORF_v4|nuORFdb_human IFRD1_7:112090542-112090700:+ IFRD1_7:112090542-112090700:+|ENST00000403825.7_1_7:112090541-112090697:+ IFRD1 uORF 7:112090542-112090700:+ 7 IFRD1 5' uORF 5' uORF GN=IFRD1 E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep1_Slot1-04_1_3496 40mio-rep2 E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep2_Slot2-5_1_3520.24471.424.1 1 1 1 10.64 4.43 4.29 6.21 82.1 6 0 0 6210.0 Hydrogen 28 (T) 9 LLLAEDSPL (S) 9 LLLnAELSP L L L\A|E-D\S\P\L 66.7 6 5 1 1 1 b-ion bias 84.534683 1.471031 970.5391 970.545 -0.0065 -6.7 -1.7999999999999998 3.66 5911.7 smORF_v4|nuORFdb_human IFRD1_7:112090542-112090700:+ IFRD1_7:112090542-112090700:+|ENST00000403825.7_1_7:112090541-112090697:+ IFRD1 uORF 7:112090542-112090700:+ 7 IFRD1 5' uORF 5' uORF GN=IFRD1 E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep2_Slot2-5_1_3520 40mio-rep3 E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep3_Slot2-5_1_3521.24499.424.1 1 2 1 13.41 7.84 4.06 5.56 82.3 6 0 0 23400.0 Hydrogen 28 (T) 9 LLLAEDSPL (S) 9 IVDVLnSPL L L L|A/E\D|S|P\L 66.7 6 5 4 3 0 dual b/y bias 84.629349 1.47092 970.5417 970.545 -0.0035 -3.6 0.9999999999999996 3.66 5911.7 smORF_v4|nuORFdb_human IFRD1_7:112090542-112090700:+ IFRD1_7:112090542-112090700:+|ENST00000403825.7_1_7:112090541-112090697:+ IFRD1 uORF 7:112090542-112090700:+ 7 IFRD1 5' uORF 5' uORF GN=IFRD1 E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep3_Slot2-5_1_3521 40mio-rep4 E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep4_Slot2-5_1_3522.24479.422.1 1 1 1 13.49 5.8 6.73 7.69 85.1 6 0 0 6010.0 Hydrogen 28 (T) 9 LLLAEDSPL (S) 9 IVDVLnSPL L L\L\A|E|D|S\P-L 77.8 7 6 3 3 1 dual b/y bias 84.548462 1.472075 970.5401 970.545 -0.0055 -5.7 -1.0 3.66 5911.7 smORF_v4|nuORFdb_human IFRD1_7:112090542-112090700:+ IFRD1_7:112090542-112090700:+|ENST00000403825.7_1_7:112090541-112090697:+ IFRD1 uORF 7:112090542-112090700:+ 7 IFRD1 5' uORF 5' uORF GN=IFRD1 E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep4_Slot2-5_1_3522 40mio-rep2 E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep2_Slot2-5_1_3520.20865.1428.2 2 1 1 12.22 8.95 2.17 3.27 81.4 8 1 1 5100.0 Hydrogen 28 (A) 9 LLLDQLTKA (L) 9 ILInDKVAK L/L|L|D/Q\L-T\K\A 100.0 9 5 4 2 1 unbiased 72.080536 0.859103 507.8109 1014.619 -0.0046 -4.6 0.3000000000000007 5.84 5493.4 nuORFdb_human ENST00000510524.5_1_3:129170769-129177513:+ ENST00000510524.5_1_3:129170769-129177513:+ IFT122 Nonsense Mediated Decay Other GN=IFT122 Nonsense Mediated Decay E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep2_Slot2-5_1_3520 40mio-rep4 E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep4_Slot2-5_1_3522.20890.1438.2 2 2 1 10.02 7.79 1.13 2.23 70.6 8 1 1 10700.0 Hydrogen 28 (A) 9 LLLDQLTKA (L) 9 ILLLDTGAKA L/L/L/D/Q\L\T\K\A 100.0 9 4 4 0 0 unbiased 72.146828 0.852314 507.8125 1014.619 -0.0026 -2.6 2.1 5.84 5493.4 nuORFdb_human ENST00000510524.5_1_3:129170769-129177513:+ ENST00000510524.5_1_3:129170769-129177513:+ IFT122 Nonsense Mediated Decay Other GN=IFT122 Nonsense Mediated Decay E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep4_Slot2-5_1_3522 40mio-rep1 E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep1_Slot1-04_1_3496.28570.1407.2 2 3 1 11.96 10.34 2.65 1.62 84.6 7 0 0 19200.0 Hydrogen 3 (L) 9 LLLETVFHL (L) 9 LLLETRKPD L/L/L|E|T|V/F H-L 77.8 7 3 6 3 1 dual b/y bias 98.701035 0.872018 542.8224 1084.64 -0.0034 -3.1 0.10000000000000009 5.24 2715.2 nuORFdb_human ENST00000546387.1_1_12:44238581-44238653:+ ENST00000546387.1_1_12:44238581-44238653:+ TMEM117 Out-of-Frame Out-of-Frame GN=TMEM117 Out-of-Frame E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep1_Slot1-04_1_3496 40mio-rep2 E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep2_Slot2-5_1_3520.28501.1410.2 2 1 1 13.84 10.65 3.66 3.19 80.1 7 0 0 4440.0 Hydrogen 3 (L) 9 LLLETVFHL (L) 9 LLLETRKPD L/L|L|E|T|V|F/H L 77.8 7 5 7 5 0 dual b/y bias 98.458145 0.870129 542.8193 1084.64 -0.0094 -8.7 -3.799999999999999 5.24 2715.2 nuORFdb_human ENST00000546387.1_1_12:44238581-44238653:+ ENST00000546387.1_1_12:44238581-44238653:+ TMEM117 Out-of-Frame Out-of-Frame GN=TMEM117 Out-of-Frame E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep2_Slot2-5_1_3520 SCP E_20221201_NO30_400nL_HLAc1_4e7_PDAC_Slot2-6_1_3524.26715.1466.2 2 1 1 8.32 7.12 1.18 1.2 71.1 5 0 0 11100.0 Hydrogen 38 (P) 9 LLLLSNSPP (P) 9 qEKLNLIL L/L|L/L-S N\S\P P 55.6 5 3 3 1 1 unbiased 92.294014 0.835227 477.286 953.567 -0.0019 -2.0 0.3999999999999999 5.52 5768.5 nuORFdb_human ENST00000245479.2_1_17:70117189-70117348:+ ENST00000245479.2_1_17:70117189-70117348:+ SOX9 5' uORF 5' uORF GN=SOX9 5' uORF E_20221201_NO30_400nL_HLAc1_4e7_PDAC_Slot2-6_1_3524 20mio-rep1 E_20221201_NO30_400nL_HLAc1_2e7_directIP_titration_rep1_Slot2-4_1_3515.20327.467.1 1 1 1 11.97 7.58 4.53 4.39 87.7 6 0 0 2620.0 Hydrogen 21 (T) 9 LLPDSTPSL (L) 9 LLPnTSPIS L L/P D\S|T|P-S\L 55.6 5 4 3 2 1 unbiased 70.197556 1.445541 942.5103 942.514 -0.0042 -4.5 1.7000000000000002 3.8 3323.9 smORF_v4|nuORFdb_human TRIM62_1:33647158-33647253:- TRIM62_1:33647158-33647253:-|ENST00000291416.9_1_1:33647160-33647253:- TRIM62 uORF 1:33647158-33647253:- 1 TRIM62 5' uORF 5' uORF GN=TRIM62 E_20221201_NO30_400nL_HLAc1_2e7_directIP_titration_rep1_Slot2-4_1_3515 20mio-rep2 E_20221201_NO30_400nL_HLAc1_2e7_directIP_titration_rep3_Slot2-4_1_3518.20361.462.1 1 1 1 10.57 7.5 3.07 3.07 86.7 6 0 0 2300.0 Hydrogen 21 (T) 9 LLPDSTPSL (L) 9 LLPnTSPIS L L/P\D S|T|P-S\L 55.6 5 4 3 2 1 unbiased 70.331467 1.448856 942.51 942.514 -0.0042 -4.5 1.7999999999999998 3.8 3323.9 smORF_v4|nuORFdb_human TRIM62_1:33647158-33647253:- TRIM62_1:33647158-33647253:-|ENST00000291416.9_1_1:33647160-33647253:- TRIM62 uORF 1:33647158-33647253:- 1 TRIM62 5' uORF 5' uORF GN=TRIM62 E_20221201_NO30_400nL_HLAc1_2e7_directIP_titration_rep3_Slot2-4_1_3518 40mio-rep2 E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep2_Slot2-5_1_3520.20305.454.1 1 1 1 11.39 9.08 2.56 2.31 87.0 7 0 0 5820.0 Hydrogen 21 (T) 9 LLPDSTPSL (L) 9 LLPnTSPIS L/L/P D/S/T|P\S\L 77.8 7 3 5 1 0 unbiased 70.143822 1.453412 942.5085 942.514 -0.0052 -5.6 -0.6999999999999993 3.8 3323.9 smORF_v4|nuORFdb_human TRIM62_1:33647158-33647253:- TRIM62_1:33647158-33647253:-|ENST00000291416.9_1_1:33647160-33647253:- TRIM62 uORF 1:33647158-33647253:- 1 TRIM62 5' uORF 5' uORF GN=TRIM62 E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep2_Slot2-5_1_3520 40mio-rep4 E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep4_Slot2-5_1_3522.20306.452.1 1 1 1 7.72 6.03 1.5 1.69 81.6 6 0 0 5400.0 Hydrogen 21 (T) 9 LLPDSTPSL (L) 9 LITPDSSLP L L/P D\S-T|P-S\L 55.6 5 3 2 1 2 unbiased 70.130234 1.454455 942.5073 942.514 -0.0072 -7.7 -3.0 3.8 3323.9 smORF_v4|nuORFdb_human TRIM62_1:33647158-33647253:- TRIM62_1:33647158-33647253:-|ENST00000291416.9_1_1:33647160-33647253:- TRIM62 uORF 1:33647158-33647253:- 1 TRIM62 5' uORF 5' uORF GN=TRIM62 E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep4_Slot2-5_1_3522 40mio-rep1 E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep1_Slot1-04_1_3496.16785.1325.2 2 1 1 9.02 7.5 1.6 1.52 73.1 6 0 0 7170.0 Hydrogen 1 (-) 10 LLPECCTIFG (-) 10 ILPGMVcTSAV L/L|P|E/C C-T I F-G 50.0 5 2 4 2 2 y-ion bias Carbamidomethylation C:Carbamidomethylation, 57.980099 0.923067 605.2841 1209.564 -0.0035 -2.9 0.30000000000000027 4.0 1209.4 nuORFdb_human ENST00000399878.7_2_4:25849463-25864306:- ENST00000399878.7_2_4:25849463-25864306:- SEL1L3 Out-of-Frame Out-of-Frame GN=SEL1L3 Out-of-Frame E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep1_Slot1-04_1_3496 SCP E_20221201_NO30_400nL_HLAc1_4e7_PDAC_Slot2-6_1_3524.30285.303.1 1 1 1 12.23 10.68 1.62 1.56 90.7 7 0 0 94500.0 Hydrogen 9 (W) 9 LLPEILIPI (T) 9 PELLIILPL L L/P\E|I\L\I-P\I 77.8 7 5 2 1 1 b-ion bias 104.632584 1.542563 1020.6679 1020.67 -0.0003 -0.3 2.1 4.0 26510.9 nuORFdb_human ENST00000474457.1_1_6:52956864-52957704:+ ENST00000474457.1_1_6:52956864-52957704:+ FBXO9 ncRNA Retained Intron lncRNA GN=FBXO9 ncRNA Retained Intron E_20221201_NO30_400nL_HLAc1_4e7_PDAC_Slot2-6_1_3524 SCP E_20221201_NO30_400nL_HLAc1_4e7_PDAC_Slot2-6_1_3524.13882.1246.2 2 3 1 10.54 7.74 2.8 2.8 88.3 7 1 1 17500.0 Hydrogen 32 (V) 9 LLPHQYPRW (-) 9 LIPHTDGEKLS L/L|P/H|Q/Y/P R\W 77.8 7 3 6 2 0 y-ion bias 47.958084 0.972673 605.3293 1209.653 -0.002 -1.7 0.7 8.75 4617.4 nuORFdb_human ENST00000254654.7_1_2:239098583-239103478:- ENST00000254654.7_1_2:239098583-239103478:-|ENST00000467133.5_1_2:239098583-239112329:- ILKAP Out-of-Frame Out-of-Frame GN=ILKAP Out-of-Frame E_20221201_NO30_400nL_HLAc1_4e7_PDAC_Slot2-6_1_3524 SCP E_20221201_NO30_400nL_HLAc1_4e7_PDAC_Slot2-6_1_3524.30384.289.1 1 1 1 9.15 6.88 3.7 2.27 75.9 6 0 0 376000.0 Hydrogen 6 (K) 9 LLSDTLVLL (I) 9 VIESLLSLL L L S\D\T\L\V\L\L 66.7 6 6 0 0 0 b-ion bias 104.974945 1.55052 986.6135 986.613 -0.0002 -0.2 2.1999999999999997 3.8 5083.0 nuORFdb_human ENST00000258341.4_1_1:183113356-183113482:+ ENST00000258341.4_1_1:183113356-183113482:+ LAMC1 3' dORF 3' dORF GN=LAMC1 3' dORF E_20221201_NO30_400nL_HLAc1_4e7_PDAC_Slot2-6_1_3524 40mio-rep2 E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep2_Slot2-5_1_3520.29170.335.1 1 1 1 7.54 4.14 4.32 3.4 70.4 6 0 0 5070.0 Hydrogen 35 (W) 9 LLSPFDMTL (-) 9 LLSGmGATGTL L L/S/P F-D\M\T\L 55.6 5 3 2 0 1 unbiased 100.768875 1.523898 1036.533 1036.538 -0.0083 -8.1 -3.1999999999999993 3.8 5048.0 nuORFdb_human T217878_20:39624293-39625887:- T217878_20:39624293-39625887:-|T217865_20:39624293-39633887:- lincRNA lncRNA GN=nan lincRNA E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep2_Slot2-5_1_3520 40mio-rep1 E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep1_Slot1-04_1_3496.27067.306.1 1 1 1 12.1 5.18 5.32 6.92 79.0 6 0 0 4220.0 M41m Hydrogen 35 (W) 9 LLSPFDmTL (-) 9 LLSMLnDFV L L/S/P F\D\m\T\L 55.6 5 4 2 0 0 b-ion bias m:Oxidized methionine 93.50927 1.541007 1052.5324 1036.538 15.9917 -3.1 0.10000000000000009 3.8 5048.0 nuORFdb_human T217878_20:39624293-39625887:- T217878_20:39624293-39625887:-|T217865_20:39624293-39633887:- lincRNA lncRNA GN=nan lincRNA E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep1_Slot1-04_1_3496 40mio-rep2 E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep2_Slot2-5_1_3520.26819.319.1 1 1 1 9.6 3.97 6.4 5.62 71.0 6 0 0 2590.0 M41m Hydrogen 35 (W) 9 LLSPFDmTL (-) 9 LLSMLnDFV L L/S/P F/D|m\T\L 55.6 5 3 4 1 0 unbiased m:Oxidized methionine 92.647865 1.53287 1052.5275 1036.538 15.9917 -3.1 1.8000000000000003 3.8 5048.0 nuORFdb_human T217878_20:39624293-39625887:- T217878_20:39624293-39625887:-|T217865_20:39624293-39633887:- lincRNA lncRNA GN=nan lincRNA E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep2_Slot2-5_1_3520 40mio-rep1 E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep1_Slot1-04_1_3496.23131.248.1 1 1 1 14.02 4.25 10.66 9.77 84.2 8 0 0 2900.0 Hydrogen 91 (P) 10 LLWEDGVTEA (V) 10 AAAKAEAEATAE L L/W|E|D\G\V\T\E\A 80.0 8 7 3 2 0 b-ion bias 79.907883 1.574616 1132.5498 1132.552 -0.0021 -1.8 1.4000000000000001 3.57 11308.9 nuORFdb_human ENST00000452404.6_1_3:196666301-196669112:- ENST00000452404.6_1_3:196666301-196669112:- NCBP2 5' Overlap uORF 5' Overlap uORF GN=NCBP2 5' Overlap uORF E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep1_Slot1-04_1_3496 40mio-rep2 E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep2_Slot2-5_1_3520.22857.1391.2 2 1 1 8.44 3.37 4.96 5.08 65.5 7 0 0 6820.0 Hydrogen 91 (P) 10 LLWEDGVTEA (V) 10 AAAKAEAEATAE L L W\E\D\G\V|T\E-A 70.0 7 6 1 1 1 b-ion bias 78.960732 0.882149 566.7783 1132.552 -0.0034 -3.0 1.9000000000000004 3.57 11308.9 nuORFdb_human ENST00000452404.6_1_3:196666301-196669112:- ENST00000452404.6_1_3:196666301-196669112:- NCBP2 5' Overlap uORF 5' Overlap uORF GN=NCBP2 5' Overlap uORF E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep2_Slot2-5_1_3520 40mio-rep4 E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep4_Slot2-5_1_3522.22848.1398.2 2 1 1 10.2 4.19 3.67 6.01 67.1 7 0 0 4240.0 Hydrogen 91 (P) 10 LLWEDGVTEA (V) 10 ERDALnIETA L L/W\E\D G\V|T\E\A 60.0 6 6 2 1 0 b-ion bias 78.915031 0.877818 566.7769 1132.552 -0.0054 -4.7 0.0 3.57 11308.9 nuORFdb_human ENST00000452404.6_1_3:196666301-196669112:- ENST00000452404.6_1_3:196666301-196669112:- NCBP2 5' Overlap uORF 5' Overlap uORF GN=NCBP2 5' Overlap uORF E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep4_Slot2-5_1_3522 40mio-rep1 E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep1_Slot1-04_1_3496.27866.244.1 1 2 1 17.43 8.37 9.55 9.06 89.7 7 0 0 11300.0 M24m Hydrogen 17 (N) 9 LLWEFPSmA (-) 9 LVFNQSPSmA L L/W|E|F|P|S|m-A 77.8 7 5 6 5 1 dual b/y bias m:Oxidized methionine 96.26889 1.576974 1109.5301 1093.539 15.9913 -3.2 0.0 4.0 3067.7 nuORFdb_human ENST00000360635.7_1_2:9548264-9552405:- ENST00000360635.7_1_2:9548264-9552405:- ITGB1BP1 Out-of-Frame Out-of-Frame GN=ITGB1BP1 Out-of-Frame E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep1_Slot1-04_1_3496 40mio-rep2 E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep2_Slot2-5_1_3520.27752.270.1 1 2 1 15.71 8.92 7.38 6.78 89.5 6 0 0 11500.0 M24m Hydrogen 17 (N) 9 LLWEFPSmA (-) 9 LVFNQSPSmA L L/W/E|F|P|S m\A 55.6 5 4 5 3 0 dual b/y bias m:Oxidized methionine 95.872475 1.561984 1109.5317 1093.539 15.9913 -3.2 1.7000000000000002 4.0 3067.7 nuORFdb_human ENST00000360635.7_1_2:9548264-9552405:- ENST00000360635.7_1_2:9548264-9552405:- ITGB1BP1 Out-of-Frame Out-of-Frame GN=ITGB1BP1 Out-of-Frame E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep2_Slot2-5_1_3520 40mio-rep3 E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep3_Slot2-5_1_3521.27753.271.1 1 2 1 15.68 8.37 6.85 7.31 90.2 7 0 0 10500.0 M24m Hydrogen 17 (N) 9 LLWEFPSmA (-) 9 LVFNQSPSmA L L/W/E|F|P\S\m\A 77.8 7 5 4 2 0 unbiased m:Oxidized methionine 95.868515 1.561322 1109.5284 1093.539 15.9913 -3.2 1.3999999999999995 4.0 3067.7 nuORFdb_human ENST00000360635.7_1_2:9548264-9552405:- ENST00000360635.7_1_2:9548264-9552405:- ITGB1BP1 Out-of-Frame Out-of-Frame GN=ITGB1BP1 Out-of-Frame E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep3_Slot2-5_1_3521 40mio-rep4 E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep4_Slot2-5_1_3522.27764.1425.2 2 3 1 17.63 6.14 12.14 11.49 89.9 6 0 0 14300.0 M24m Hydrogen 17 (N) 9 LLWEFPSmA (-) 9 LVFNQSPSmA L L|W|E|F|P/S m\A 55.6 5 5 5 4 0 dual b/y bias m:Oxidized methionine 95.896141 0.860644 555.2672 1093.539 15.988 -6.2 -1.5 4.0 3067.7 nuORFdb_human ENST00000360635.7_1_2:9548264-9552405:- ENST00000360635.7_1_2:9548264-9552405:- ITGB1BP1 Out-of-Frame Out-of-Frame GN=ITGB1BP1 Out-of-Frame E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep4_Slot2-5_1_3522 Exploris_FAIMS VH20221207_MKP_FAIMS2022E_HLAc1_4e7_PDAC.33354.33354.2 2 1 1 11.46 9.18 3.55 2.28 86.9 5 0 1 37300000.0 Hydrogen 22 (S) 10 LPAAGAPFRP (R) 10 LPAAARARNG L/P|A|A/G A P-F R P 40.0 4 2 4 2 1 y-ion bias 0.993 75.8 63.8 27.18 -50.0 498.7856 996.562 0.0013 1.3 3.9000000000000004 9.75 6066.9 nuORFdb_human ENST00000244565.7_1_6:40995845-40996025:- ENST00000244565.7_1_6:40995845-40996025:- UNC5CL 3' dORF 3' dORF GN=UNC5CL 3' dORF VH20221207_MKP_FAIMS2022E_HLAc1_4e7_PDAC SCP E_20221201_NO30_400nL_HLAc1_4e7_PDAC_Slot2-6_1_3524.11896.1273.2 2 1 1 11.54 7.78 3.32 3.76 81.1 8 1 2 10200.0 Hydrogen 45 (L) 10 LPACVRKVSL (R) 10 LPEKETLLSL L/P/A|C/V/R K-V\S\L 80.0 8 3 5 1 1 unbiased Carbamidomethylation C:Carbamidomethylation, 41.09716 0.95555 571.8388 1142.671 -0.0007 -0.6 1.7999999999999998 11.0 9492.8 nuORFdb_human TCONS_00010584_5:180258225-180258501:- TCONS_00010584_5:180258225-180258501:- linc-MGAT1-2 lincRNA lncRNA GN=linc-MGAT1-2 lincRNA E_20221201_NO30_400nL_HLAc1_4e7_PDAC_Slot2-6_1_3524 Exploris_FAIMS VH20221207_MKP_FAIMS2022E_HLAc1_4e7_PDAC.31502.31502.3 3 1 1 12.4 6.46 5.33 5.94 78.8 7 1 2 21400000.0 Hydrogen 45 (L) 10 LPACVRKVSL (R) 10 PALRKmQGIL L/P/A/C/V R K-V/S/L 70.0 7 0 6 0 1 y-ion bias Carbamidomethylation C:Carbamidomethylation, 0.989 83.8 60.98 24.15 -50.0 381.5612 1142.671 -0.0024 -2.1 0.5 11.0 9492.8 nuORFdb_human TCONS_00010584_5:180258225-180258501:- TCONS_00010584_5:180258225-180258501:- linc-MGAT1-2 lincRNA lncRNA GN=linc-MGAT1-2 lincRNA VH20221207_MKP_FAIMS2022E_HLAc1_4e7_PDAC Eva-50mio-3fr AL20210827_EV_HLA_PDAC_PANFRO413_2D_50e6_W632_classI_fxn1.27246.27246.2 2 2 1 16.42 10.39 3.57 6.03 93.5 7 0 0 13200000.0 Hydrogen 79 (L) 9 LPAEEAPAL (R) 9 PVVnEAPAI L P\A\E\E\A|P/A|L 77.8 7 6 3 2 0 b-ion bias 0.999 100.0 49.5 91.31 0.0 455.7484 910.488 0.0014 1.5 1.4 3.8 15830.8 nuORFdb_human ENST00000463441.5_1_7:150020319-150023055:+ ENST00000463441.5_1_7:150020319-150023055:+|ENST00000493307.1_1_7:150020415-150021458:+|ENST00000323078.7_1_7:150020415-150033884:+ LRRC61 ncRNA Processed Transcript lncRNA GN=LRRC61 ncRNA Processed Transcript AL20210827_EV_HLA_PDAC_PANFRO413_2D_50e6_W632_classI_fxn1 SCP E_20221201_NO30_400nL_HLAc1_4e7_PDAC_Slot2-6_1_3524.16997.530.1 1 3 1 16.5 11.52 5.52 4.98 94.3 7 0 0 64200.0 Hydrogen 79 (L) 9 LPAEEAPAL (R) 9 PVVnEAPAI L/P A|E\E|A|P\A\L 77.8 7 6 4 3 0 dual b/y bias 58.719894 1.408166 910.4852 910.488 -0.003 -3.3 -0.8999999999999999 3.8 15830.8 nuORFdb_human ENST00000463441.5_1_7:150020319-150023055:+ ENST00000463441.5_1_7:150020319-150023055:+|ENST00000493307.1_1_7:150020415-150021458:+|ENST00000323078.7_1_7:150020415-150033884:+ LRRC61 ncRNA Processed Transcript lncRNA GN=LRRC61 ncRNA Processed Transcript E_20221201_NO30_400nL_HLAc1_4e7_PDAC_Slot2-6_1_3524 SCP E_20221201_NO30_400nL_HLAc1_4e7_PDAC_Slot2-6_1_3524.15029.1459.2 2 1 1 12.3 7.97 3.93 4.33 66.1 8 1 1 13700.0 Hydrogen 12 (S) 9 LPAETPKLY (S) 9 LPGALDGIIY L/P/A/E/T/P/K\L\Y 100.0 9 2 6 0 0 y-ion bias 51.92284 0.839334 516.2897 1031.577 -0.0045 -4.3 -1.9 6.0 2578.9 nuORFdb_human ENST00000530022.5_1_11:8704360-8704432:+ ENST00000530022.5_1_11:8704360-8704432:+ RPL27A 5' Overlap uORF 5' Overlap uORF GN=RPL27A 5' Overlap uORF E_20221201_NO30_400nL_HLAc1_4e7_PDAC_Slot2-6_1_3524 Exploris_FAIMS VH20221207_MKP_FAIMS2022E_HLAc1_4e7_PDAC.38137.38137.2 2 2 1 16.05 6.75 9.81 9.31 86.0 8 1 1 38900000.0 Hydrogen 12 (S) 9 LPAETPKLY (S) 9 LPGALDGIIY L/P|A/E/T/P-K|L/Y 100.0 9 2 7 2 1 y-ion bias 0.977 83.9 70.22 42.11 -70.0 516.2907 1031.577 -0.003 -2.9 -0.2999999999999998 6.0 2578.9 nuORFdb_human ENST00000530022.5_1_11:8704360-8704432:+ ENST00000530022.5_1_11:8704360-8704432:+ RPL27A 5' Overlap uORF 5' Overlap uORF GN=RPL27A 5' Overlap uORF VH20221207_MKP_FAIMS2022E_HLAc1_4e7_PDAC SCP E_20221201_NO30_400nL_HLAc1_4e7_PDAC_Slot2-6_1_3524.16995.485.1 1 1 1 11.51 9.53 3.19 1.98 88.3 7 0 0 5500.0 Hydrogen 53 (G) 9 LPASHLAVL (R) 9 APHSLAIVL L/P A/S/H\L\A\V\L 77.8 7 4 3 0 0 unbiased 58.713001 1.434628 920.552 920.556 -0.0044 -4.8 -2.4 6.74 12764.0 nuORFdb_human ENST00000288985.11_1_9:98638084-98643219:+ ENST00000288985.11_1_9:98638084-98643219:+ ERCC6L2 5' Overlap uORF 5' Overlap uORF GN=ERCC6L2 5' Overlap uORF E_20221201_NO30_400nL_HLAc1_4e7_PDAC_Slot2-6_1_3524 Exploris_FAIMS VH20221207_MKP_FAIMS2022E_HLAc1_4e7_PDAC.58330.58330.2 2 1 1 9.97 4.84 5.0 5.13 71.6 6 0 0 2360000.0 Hydrogen 60 (L) 9 LPCAGAWSL (L) 9 mATHWLSL L P C\A\G\A\W|S/L 66.7 6 5 2 1 0 b-ion bias Carbamidomethylation C:Carbamidomethylation, 0.986 87.1 101.6 120.0 -70.0 487.7399 974.476 -0.0038 -3.9 -1.2999999999999998 5.52 9686.2 nuORFdb_human ENST00000417226.6_1_3:128998609-129007820:+ ENST00000417226.6_1_3:128998609-129007820:+ HMCES Out-of-Frame Out-of-Frame GN=HMCES Out-of-Frame VH20221207_MKP_FAIMS2022E_HLAc1_4e7_PDAC Eva-50mio-3fr AL20210827_EV_HLA_PDAC_PANFRO413_2D_50e6_W632_classI_fxn1.21102.21102.2 2 1 1 13.91 10.18 4.76 3.72 78.7 7 1 1 85600000.0 Hydrogen 31 (C) 9 LPDPEDRSF (R) 9 LPnKNDPNY L/P|D|P/E/D/R S/F 77.8 7 2 7 2 0 y-ion bias 0.999 61.5 39.98 69.71 0.0 538.254 1075.505 -0.0047 -4.4 -4.5 4.03 7312.1 nuORFdb_human ENST00000362000.9_1_7:65540928-65547388:+ ENST00000362000.9_1_7:65540928-65547388:+ ASL 5' Overlap uORF 5' Overlap uORF GN=ASL 5' Overlap uORF AL20210827_EV_HLA_PDAC_PANFRO413_2D_50e6_W632_classI_fxn1 Exploris AL20221201_MKP_PDAC_01.27929.27929.2 2 1 1 10.35 7.48 3.01 2.87 60.4 6 1 1 223000000.0 Hydrogen 31 (C) 9 LPDPEDRSF (R) 9 LPDSPERFD L/P/D|P/E/D/R S F 66.7 6 1 6 1 0 y-ion bias 0.999 48.8 44.92 76.15 0.0 538.255 1075.505 -0.0027 -2.5 -3.3 4.03 7312.1 nuORFdb_human ENST00000362000.9_1_7:65540928-65547388:+ ENST00000362000.9_1_7:65540928-65547388:+ ASL 5' Overlap uORF 5' Overlap uORF GN=ASL 5' Overlap uORF AL20221201_MKP_PDAC_01 Exploris_FAIMS VH20221207_MKP_FAIMS2022E_HLAc1_4e7_PDAC.31190.31190.3 3 1 1 11.42 5.97 6.91 5.44 79.1 6 1 1 2430000.0 Hydrogen 21 (G) 9 LPERVHEVF (R) 9 nIHIPRnLF L/P/E R-V H\E|V/F 55.6 5 2 4 1 1 y-ion bias 0.975 97.5 60.57 90.47 -70.0 375.8723 1125.605 -0.0028 -2.5 0.10000000000000009 5.4 4047.8 smORF_v4|nuORFdb_human CUL5_11:107879767-107879873:+ CUL5_11:107879767-107879873:+|ENST00000393094.6_1_11:107879697-107879871:+ CUL5 uORF 11:107879767-107879873:+ 11 CUL5 5' uORF 5' uORF GN=CUL5 VH20221207_MKP_FAIMS2022E_HLAc1_4e7_PDAC SCP E_20221201_NO30_400nL_HLAc1_4e7_PDAC_Slot2-6_1_3524.28935.552.1 1 1 1 7.96 5.97 1.68 1.99 80.7 6 0 0 13000.0 Hydrogen 9 (E) 8 LPFGGLLY (L) 8 LMGILGIY L/P F\G\G\L\L\Y 75.0 6 5 1 0 0 b-ion bias 99.966873 1.395035 879.4947 879.497 -0.0025 -2.8 -0.3999999999999999 5.52 3273.9 nuORFdb_human ENST00000491710.5_3_6:12015770-12015848:+ ENST00000491710.5_3_6:12015770-12015848:+ HIVEP1 5' uORF 5' uORF GN=HIVEP1 5' uORF E_20221201_NO30_400nL_HLAc1_4e7_PDAC_Slot2-6_1_3524 Eva-50mio-3fr AL20210827_EV_HLA_PDAC_PANFRO413_2D_50e6_W632_classI_fxn3.55030.55030.2 2 1 1 10.03 2.62 6.18 7.41 69.7 7 0 0 3100000.0 Hydrogen 25 (F) 11 LPFLTTEVNVL (C) 11 VNVLPDISLYL L P\F\L-T\T E\V\N|V/L 63.6 7 6 2 1 1 b-ion bias 0.984 88.8 99.02 100.0 0.0 623.3603 1245.709 0.0044 3.5 3.0 4.0 5158.9 nuORFdb_human ENST00000484157.5_1_1:155108136-155110067:+ ENST00000484157.5_1_1:155108136-155110067:+|ENST00000490276.5_1_1:155108315-155110067:+ SLC50A1 5' Overlap uORF 5' Overlap uORF GN=SLC50A1 5' Overlap uORF AL20210827_EV_HLA_PDAC_PANFRO413_2D_50e6_W632_classI_fxn3 Exploris AL20221201_MKP_PDAC_01.67469.67469.2 2 1 1 14.04 3.75 9.03 10.29 84.4 8 0 0 38800000.0 Hydrogen 25 (F) 11 LPFLTTEVNVL (C) 11 VNVLPDISLYL L P\F\L\T\T E\V\N|V/L 63.6 7 7 2 1 0 b-ion bias 0.978 100.0 100.73 46.67 0.0 623.3603 1245.709 0.0044 3.5 2.7 4.0 5158.9 nuORFdb_human ENST00000484157.5_1_1:155108136-155110067:+ ENST00000484157.5_1_1:155108136-155110067:+|ENST00000490276.5_1_1:155108315-155110067:+ SLC50A1 5' Overlap uORF 5' Overlap uORF GN=SLC50A1 5' Overlap uORF AL20221201_MKP_PDAC_01 Exploris_FAIMS VH20221207_MKP_FAIMS2022E_HLAc1_4e7_PDAC.65182.65182.2 2 1 1 12.52 3.04 9.48 9.48 84.3 7 0 0 11900000.0 Hydrogen 25 (F) 11 LPFLTTEVNVL (C) 11 VNVLPDISLYL L P\F\L-T\T E\V\N|V/L 63.6 7 6 2 1 1 b-ion bias 0.988 88.4 111.12 12.97 -50.0 623.3564 1245.709 -0.0034 -2.7 -0.10000000000000009 4.0 5158.9 nuORFdb_human ENST00000484157.5_1_1:155108136-155110067:+ ENST00000484157.5_1_1:155108136-155110067:+|ENST00000490276.5_1_1:155108315-155110067:+ SLC50A1 5' Overlap uORF 5' Overlap uORF GN=SLC50A1 5' Overlap uORF VH20221207_MKP_FAIMS2022E_HLAc1_4e7_PDAC SCP E_20221201_NO30_400nL_HLAc1_4e7_PDAC_Slot2-6_1_3524.18118.1336.2 2 1 1 8.09 4.12 3.47 3.96 60.1 7 1 1 5640.0 Hydrogen 34 (S) 10 LPFSNFKQSY (F) 10 YTNLEmAFIL L/P/F/S N/F/K\Q\S\Y 80.0 8 3 5 0 0 unbiased 62.592049 0.916392 615.8122 1230.615 0.0014 1.1 3.5 8.59 5998.8 nuORFdb_human ENST00000262187.9_1_7:151163972-151164125:- ENST00000262187.9_1_7:151163972-151164125:- RHEB 3' dORF 3' dORF GN=RHEB 3' dORF E_20221201_NO30_400nL_HLAc1_4e7_PDAC_Slot2-6_1_3524 SCP E_20221201_NO30_400nL_HLAc1_4e7_PDAC_Slot2-6_1_3524.28427.1277.2 2 1 1 12.32 4.93 6.79 7.39 72.7 7 1 1 16100.0 Hydrogen 7 (R) 11 LPFVPIKDLQM (E) 11 LPFVLQEAnRL L/P F|V|P/I\K D L\Q\M 54.5 6 5 4 2 0 unbiased 98.209885 0.953205 650.8694 1300.733 -0.0026 -2.0 0.3999999999999999 5.84 8869.1 smORF_v4|nuORFdb_human FBXO5_6:153304369-153296756:- FBXO5_6:153304369-153296756:-|ENST00000367241.3_1_6:153296752-153304655:- FBXO5 overlapping.uORF 6:153304369-153296756:- 6 FBXO5 5' Overlap uORF 5' Overlap uORF GN=FBXO5 E_20221201_NO30_400nL_HLAc1_4e7_PDAC_Slot2-6_1_3524 Eva-50mio-3fr AL20210827_EV_HLA_PDAC_PANFRO413_2D_50e6_W632_classI_fxn1.20249.20249.2 2 1 1 13.05 6.19 6.65 6.86 71.9 6 0 0 92200000.0 M86m Hydrogen 78 (V) 9 LPHEASFVm (A) 9 YPVHGVEVm L P H\E\A\S\F|V|m 66.7 6 6 2 2 0 b-ion bias m:Oxidized methionine 0.931 80.8 37.7 100.0 0.0 523.7535 1030.503 15.9971 2.0 1.9 5.24 10913.6 nuORFdb_human ENST00000494664.5_1_3:119217725-119222457:+ ENST00000494664.5_1_3:119217725-119222457:+ TIMMDC1 Out-of-Frame Out-of-Frame GN=TIMMDC1 Out-of-Frame AL20210827_EV_HLA_PDAC_PANFRO413_2D_50e6_W632_classI_fxn1 Eva-50mio-3fr AL20210827_EV_HLA_PDAC_PANFRO413_2D_50e6_W632_classI_fxn2.22983.22983.2 2 6 3 15.22 6.45 10.38 8.77 81.9 7 1 1 294000000.0 Hydrogen 817 (T) 9 LPHFIKDDY (G) 9 PLHmTKEIY L/P|H|F|I/K D/D/Y 77.8 7 3 7 3 0 dual b/y bias 0.776 95.0 42.63 82.9 0.0 574.2936 1147.578 0.0016 1.4 1.0999999999999999 5.21 213870.4 nuORFdb_human ENST00000617998.4_2_17:7388053-7417318:+ ENST00000617998.4_2_17:7388053-7417318:+ POLR2A 5' Overlap uORF 5' Overlap uORF GN=POLR2A 5' Overlap uORF AL20210827_EV_HLA_PDAC_PANFRO413_2D_50e6_W632_classI_fxn2 Exploris AL20221201_MKP_PDAC_01.30072.30072.2 2 4 1 13.15 6.14 9.19 7.01 73.8 7 1 1 2390000000.0 Hydrogen 817 (T) 9 LPHFIKDDY (G) 9 PLHmTKEIY L/P|H|F/I/K D/D/Y 77.8 7 2 7 2 0 y-ion bias 0.821 89.8 47.92 10.07 0.0 574.2932 1147.578 0.0009 0.8 0.0 5.21 213870.4 nuORFdb_human ENST00000617998.4_2_17:7388053-7417318:+ ENST00000617998.4_2_17:7388053-7417318:+ POLR2A 5' Overlap uORF 5' Overlap uORF GN=POLR2A 5' Overlap uORF AL20221201_MKP_PDAC_01 SCP E_20221201_NO30_400nL_HLAc1_4e7_PDAC_Slot2-6_1_3524.13928.1340.2 2 5 1 16.83 10.03 7.86 6.8 92.4 8 1 1 153000.0 Hydrogen 817 (T) 9 LPHFIKDDY (G) 9 LPYSTFRTY L/P/H|F|I|K\D\D\Y 100.0 9 6 5 3 0 dual b/y bias 48.116909 0.913862 574.2913 1147.578 -0.0035 -3.1 -0.7000000000000002 5.21 213870.4 nuORFdb_human ENST00000617998.4_2_17:7388053-7417318:+ ENST00000617998.4_2_17:7388053-7417318:+ POLR2A 5' Overlap uORF 5' Overlap uORF GN=POLR2A 5' Overlap uORF E_20221201_NO30_400nL_HLAc1_4e7_PDAC_Slot2-6_1_3524 Exploris_FAIMS VH20221207_MKP_FAIMS2022E_HLAc1_4e7_PDAC.36691.36823.2 2 7 1 15.91 7.32 14.12 8.59 86.6 6 1 1 715000000.0 Hydrogen 817 (T) 9 LPHFIKDDY (G) 9 PLHPETYKY L/P|H|F|I/K D D/Y 66.7 6 3 6 3 0 dual b/y bias 0.815 97.2 68.03 12.68 -50.0 574.2912 1147.578 -0.0031 -2.7 -0.10000000000000009 5.21 213870.4 nuORFdb_human ENST00000617998.4_2_17:7388053-7417318:+ ENST00000617998.4_2_17:7388053-7417318:+ POLR2A 5' Overlap uORF 5' Overlap uORF GN=POLR2A 5' Overlap uORF VH20221207_MKP_FAIMS2022E_HLAc1_4e7_PDAC Exploris AL20221201_MKP_PDAC_01.17630.17630.3 3 1 1 7.79 7.79 7.79 61.4 6 0 0 26900000.0 Hydrogen 47 (S) 9 LPLAHHHLY (F) 9 L/P|L/A H H/H\L/Y 66.7 6 2 5 1 0 y-ion bias 0.631 66.0 31.0 29.72 0.0 367.538 1100.6 -0.0006 -0.6 -1.4 7.02 8586.7 nuORFdb_human ENST00000406386.7_1_22:38136963-38150984:+ ENST00000406386.7_1_22:38136963-38150984:+|ENST00000407319.6_1_22:38142252-38150984:+|ENST00000413051.2_1_22:38146679-38150984:+|ENST00000418339.5_2_22:38147125-38150984:+|ENST00000452519.5_2_22:38147480-38150984:+|ENST00000417857.1_2_22:38147480-38150984:+ TRIOBP Out-of-Frame Out-of-Frame GN=TRIOBP Out-of-Frame AL20221201_MKP_PDAC_01 SCP E_20221201_NO30_400nL_HLAc1_4e7_PDAC_Slot2-6_1_3524.7718.1329.2 2 1 1 18.15 7.35 12.06 10.8 91.9 8 0 0 6670.0 Hydrogen 47 (S) 9 LPLAHHHLY (F) 9 LPLPAIQAnY L/P/L|A|H/H|H|L\Y 100.0 9 5 7 4 0 dual b/y bias 26.665819 0.920632 550.8024 1100.6 -0.0033 -3.0 -0.6000000000000001 7.02 8586.7 nuORFdb_human ENST00000406386.7_1_22:38136963-38150984:+ ENST00000406386.7_1_22:38136963-38150984:+|ENST00000407319.6_1_22:38142252-38150984:+|ENST00000413051.2_1_22:38146679-38150984:+|ENST00000418339.5_2_22:38147125-38150984:+|ENST00000452519.5_2_22:38147480-38150984:+|ENST00000417857.1_2_22:38147480-38150984:+ TRIOBP Out-of-Frame Out-of-Frame GN=TRIOBP Out-of-Frame E_20221201_NO30_400nL_HLAc1_4e7_PDAC_Slot2-6_1_3524 Exploris_FAIMS VH20221207_MKP_FAIMS2022E_HLAc1_4e7_PDAC.22600.22600.3 3 1 1 12.53 1.45 12.53 11.08 84.4 6 0 0 12400000.0 Hydrogen 47 (S) 9 LPLAHHHLY (F) 9 LPHCIKGYL L P|L/A H-H/H/L/Y 55.6 5 1 5 1 1 y-ion bias 0.9 91.9 46.97 69.37 -50.0 367.5362 1100.6 -0.006 -5.5 -2.9 7.02 8586.7 nuORFdb_human ENST00000406386.7_1_22:38136963-38150984:+ ENST00000406386.7_1_22:38136963-38150984:+|ENST00000407319.6_1_22:38142252-38150984:+|ENST00000413051.2_1_22:38146679-38150984:+|ENST00000418339.5_2_22:38147125-38150984:+|ENST00000452519.5_2_22:38147480-38150984:+|ENST00000417857.1_2_22:38147480-38150984:+ TRIOBP Out-of-Frame Out-of-Frame GN=TRIOBP Out-of-Frame VH20221207_MKP_FAIMS2022E_HLAc1_4e7_PDAC SCP E_20221201_NO30_400nL_HLAc1_4e7_PDAC_Slot2-6_1_3524.15334.1374.2 2 1 1 11.88 6.23 4.42 5.65 84.4 7 1 1 4740.0 Hydrogen 29 (V) 9 LPLDARHVL (G) 9 IPVEHQVLV L/P/L/D|A R|H\V\L 77.8 7 4 5 2 0 unbiased 52.975971 0.892658 517.3112 1033.615 -0.0006 -0.5 1.9 6.74 8490.9 nuORFdb_human ENST00000552576.2_1_12:56123998-56124220:+ ENST00000552576.2_1_12:56123998-56124220:+ RP11-644F5.11 Antisense lncRNA GN=RP11-644F5.11 Antisense E_20221201_NO30_400nL_HLAc1_4e7_PDAC_Slot2-6_1_3524 Exploris AL20221201_MKP_PDAC_01.52822.52822.2 2 1 1 8.93 6.2 4.37 2.72 71.7 6 0 0 100000000.0 Hydrogen 39 (K) 9 LPLDVQNPL (-) 9 LPnLNLNLP L P\L\D\V\Q\N|P L 55.6 5 6 1 1 0 b-ion bias 0.941 92.7 81.15 34.16 0.0 504.7895 1008.572 -0.0007 -0.7 -1.5 3.8 5349.2 smORF_v4|nuORFdb_human RSPRY1_16:57220252-57220363:+ RSPRY1_16:57220252-57220363:+|ENST00000562439.5_1_16:57220056-57238444:+|ENST00000394420.8_1_16:57220203-57238444:+|ENST00000568505.6_1_16:57220242-57238444:+|ENST00000537866.5_1_16:57220958-57238444:+ RSPRY1 uORF 16:57220252-57220363:+ 16 RSPRY1 5' uORF 5' uORF GN=RSPRY1 AL20221201_MKP_PDAC_01 Exploris AL20221201_MKP_PDAC_01.54469.54469.1 1 1 1 10.29 5.98 7.94 4.31 78.6 7 0 0 1430000.0 Hydrogen 8 (P) 9 LPLGDAVGF (L) 9 LGLPGEVFG L/P\L-G-D\A\V\G-F 100.0 9 4 1 0 3 b-ion bias 0.922 -1.0 82.83 100.0 0.0 888.4852 888.482 0.0027 3.0 2.2 3.8 2524.9 nuORFdb_human ENST00000262713.6_1_14:23451600-23451675:- ENST00000262713.6_1_14:23451600-23451675:- AJUBA 5' uORF 5' uORF GN=AJUBA 5' uORF AL20221201_MKP_PDAC_01 SCP E_20221201_NO30_400nL_HLAc1_4e7_PDAC_Slot2-6_1_3524.25018.553.1 1 2 1 9.17 4.68 4.39 4.49 72.1 7 0 0 21300.0 Hydrogen 8 (P) 9 LPLGDAVGF (L) 9 PDGILGLGF L/P/L/G D\A\V\G\F 77.8 7 4 3 0 0 unbiased 86.429825 1.394664 888.4818 888.482 -0.0005 -0.6 1.7999999999999998 3.8 2524.9 nuORFdb_human ENST00000262713.6_1_14:23451600-23451675:- ENST00000262713.6_1_14:23451600-23451675:- AJUBA 5' uORF 5' uORF GN=AJUBA 5' uORF E_20221201_NO30_400nL_HLAc1_4e7_PDAC_Slot2-6_1_3524 Eva-50mio-3fr AL20210827_EV_HLA_PDAC_PANFRO413_2D_50e6_W632_classI_fxn3.37885.37885.2 2 1 1 11.72 8.65 3.07 3.07 72.8 6 0 0 3630000.0 Hydrogen 4 (L) 9 LPLHPLPGL (-) 9 LLPPIHPGL L/P\L H/P\L|P-G|L 77.8 7 4 4 2 1 unbiased 0.989 63.2 68.9 100.0 0.0 478.8009 956.593 0.0018 1.8 1.3 6.74 1257.6 nuORFdb_human T336781_7:155827180-155827219:- T336781_7:155827180-155827219:- G078980 lincRNA lncRNA GN=G078980 lincRNA AL20210827_EV_HLA_PDAC_PANFRO413_2D_50e6_W632_classI_fxn3 Eva-50mio-3fr AL20210827_EV_HLA_PDAC_PANFRO413_2D_50e6_W632_classI_fxn3.26155.26268.2 2 2 2 16.73 10.6 5.77 6.13 95.6 8 1 1 67300000.0 Hydrogen 2 (M) 11 LPLPSQVGSRY (L) 11 LPLPPRGDAPPS L/P|L|P/S/Q/V/G S R-Y 72.7 8 2 7 2 1 y-ion bias 0.997 100.0 48.53 69.12 0.0 608.8384 1216.668 0.001 0.8 0.30000000000000004 8.75 4047.8 smORF_v4|nuORFdb_human CUL5_11:107879767-107879873:+ CUL5_11:107879767-107879873:+|ENST00000393094.6_1_11:107879697-107879871:+ CUL5 uORF 11:107879767-107879873:+ 11 CUL5 5' uORF 5' uORF GN=CUL5 AL20210827_EV_HLA_PDAC_PANFRO413_2D_50e6_W632_classI_fxn3 Exploris AL20221201_MKP_PDAC_01.34470.34612.2 2 1 1 15.73 10.56 3.22 5.17 90.8 8 1 1 509000000.0 Hydrogen 2 (M) 11 LPLPSQVGSRY (L) 11 LPLCPLLYDL L/P|L|P/S/Q/V/G S R-Y 72.7 8 2 7 2 1 y-ion bias 1.0 90.8 54.45 11.68 0.0 608.8381 1216.668 0.0005 0.4 -0.4 8.75 4047.8 smORF_v4|nuORFdb_human CUL5_11:107879767-107879873:+ CUL5_11:107879767-107879873:+|ENST00000393094.6_1_11:107879697-107879871:+ CUL5 uORF 11:107879767-107879873:+ 11 CUL5 5' uORF 5' uORF GN=CUL5 AL20221201_MKP_PDAC_01 SCP E_20221201_NO30_400nL_HLAc1_4e7_PDAC_Slot2-6_1_3524.16073.1396.2 2 7 1 21.42 11.49 8.94 9.93 97.5 9 1 1 430000.0 Hydrogen 2 (M) 11 LPLPSQVGSRY (L) 11 LPLPPRGDAPPS L/P|L|P|S/Q|V|G S/R\Y 81.8 9 6 8 5 0 dual b/y bias 55.528461 0.878887 608.8362 1216.668 -0.0037 -3.1 -0.7000000000000002 8.75 4047.8 smORF_v4|nuORFdb_human CUL5_11:107879767-107879873:+ CUL5_11:107879767-107879873:+|ENST00000393094.6_1_11:107879697-107879871:+ CUL5 uORF 11:107879767-107879873:+ 11 CUL5 5' uORF 5' uORF GN=CUL5 E_20221201_NO30_400nL_HLAc1_4e7_PDAC_Slot2-6_1_3524 Exploris_FAIMS VH20221207_MKP_FAIMS2022E_HLAc1_4e7_PDAC.40437.40568.2 2 1 1 17.79 11.28 5.25 6.51 96.4 9 1 1 150000000.0 Hydrogen 2 (M) 11 LPLPSQVGSRY (L) 11 LPLPPRGDAPPS L/P|L|P/S/Q/V/G-S R-Y 81.8 9 2 7 2 2 y-ion bias 0.998 100.0 74.53 7.91 -70.0 608.8361 1216.668 -0.0036 -3.0 -0.3999999999999999 8.75 4047.8 smORF_v4|nuORFdb_human CUL5_11:107879767-107879873:+ CUL5_11:107879767-107879873:+|ENST00000393094.6_1_11:107879697-107879871:+ CUL5 uORF 11:107879767-107879873:+ 11 CUL5 5' uORF 5' uORF GN=CUL5 VH20221207_MKP_FAIMS2022E_HLAc1_4e7_PDAC SCP E_20221201_NO30_400nL_HLAc1_4e7_PDAC_Slot2-6_1_3524.10481.1375.2 2 1 1 10.41 5.41 5.29 5.0 76.4 7 0 0 5600.0 Hydrogen 40 (V) 9 LPLTEHNQM (N) 9 IPVYKmGQM L/P/L/T/E H\N\Q\M 77.8 7 3 4 0 0 unbiased 36.210274 0.892028 541.767 1082.53 -0.0032 -2.9 -0.5 5.24 6832.5 smORF_v4|nuORFdb_human SLC20A2_8:42396681-42330171:- SLC20A2_8:42396681-42330171:-|ENST00000524237.1_1_8:42330061-42338003:-|ENST00000517366.1_1_8:42330061-42358761:-|ENST00000518384.1_1_8:42330061-42390329:-|ENST00000342228.7_1_8:42330061-42397002:-|ENST00000520262.5_1_8:42330058-42358575:- SLC20A2 uORF 8:42396681-42330171:- 8 SLC20A2 ncRNA Processed Transcript lncRNA GN=SLC20A2 E_20221201_NO30_400nL_HLAc1_4e7_PDAC_Slot2-6_1_3524 Eva-50mio-3fr AL20210827_EV_HLA_PDAC_PANFRO413_2D_50e6_W632_classI_fxn3.55646.55646.1 1 1 1 8.65 6.43 4.3 2.22 65.1 7 0 0 798000.0 Hydrogen 33 (K) 9 LPQGIIIVF (R) 9 LPAGGILVFL L P\Q-G\I\I\I\V-F 77.8 7 5 0 0 2 b-ion bias 0.65 52.2 100.13 100.0 0.0 999.6207 999.624 -0.003 -3.0 -3.5 5.52 7977.3 smORF_v4|nuORFdb_human CASC2_10:119806552-119811682:+ CASC2_10:119806552-119811682:+|ENST00000414722.5_1_10:119806017-119811679:+|ENST00000435944.5_1_10:119806365-119811679:+ CASC2 lncRNA 10:119806552-119811682:+ 10 CASC2 Antisense lncRNA GN=CASC2 AL20210827_EV_HLA_PDAC_PANFRO413_2D_50e6_W632_classI_fxn3 SCP E_20221201_NO30_400nL_HLAc1_4e7_PDAC_Slot2-6_1_3524.29583.367.1 1 1 1 12.8 8.76 6.26 4.04 88.1 8 0 0 25100.0 Hydrogen 33 (K) 9 LPQGIIIVF (R) 9 LPAGGILVFL L/P/Q|G\I\I\I\V\F 100.0 9 6 3 1 0 b-ion bias 102.208 1.504793 999.6221 999.624 -0.0017 -1.7 0.7 5.52 7977.3 smORF_v4|nuORFdb_human CASC2_10:119806552-119811682:+ CASC2_10:119806552-119811682:+|ENST00000414722.5_1_10:119806017-119811679:+|ENST00000435944.5_1_10:119806365-119811679:+ CASC2 lncRNA 10:119806552-119811682:+ 10 CASC2 Antisense lncRNA GN=CASC2 E_20221201_NO30_400nL_HLAc1_4e7_PDAC_Slot2-6_1_3524 Exploris AL20221201_MKP_PDAC_01.35428.35428.2 2 1 1 8.77 4.3 4.54 4.47 65.2 6 1 1 187000000.0 Hydrogen 38 (G) 9 LPRDIIENY (L) 9 FPLLAPPTNY L/P R D\I\I\E|N|Y 66.7 6 5 3 2 0 unbiased 0.977 68.3 55.68 9.63 0.0 566.8041 1132.6 0.0012 1.0 0.19999999999999996 4.37 5476.1 nuORFdb_human ENST00000233143.5_1_2:85133620-85133770:+ ENST00000233143.5_1_2:85133620-85133770:+ TMSB10 3' dORF 3' dORF GN=TMSB10 3' dORF AL20221201_MKP_PDAC_01 SCP E_20221201_NO30_400nL_HLAc1_4e7_PDAC_Slot2-6_1_3524.16514.1379.2 2 2 1 14.2 4.78 7.7 9.43 89.3 6 1 1 115000.0 Hydrogen 38 (G) 9 LPRDIIENY (L) 9 PVAGGnVKLNY L P/R D\I\I|E|N\Y 55.6 5 5 3 2 0 unbiased 57.051174 0.889419 566.8017 1132.6 -0.003 -2.6 -0.20000000000000018 4.37 5476.1 nuORFdb_human ENST00000233143.5_1_2:85133620-85133770:+ ENST00000233143.5_1_2:85133620-85133770:+ TMSB10 3' dORF 3' dORF GN=TMSB10 3' dORF E_20221201_NO30_400nL_HLAc1_4e7_PDAC_Slot2-6_1_3524 Eva-50mio-3fr AL20210827_EV_HLA_PDAC_PANFRO413_2D_50e6_W632_classI_fxn1.15513.15513.3 3 1 1 7.43 6.01 -0.01 1.43 70.5 6 2 2 4100000.0 Hydrogen 18 (R) 9 LPRDREVAL (V) 9 GIHTSTRLAL L/P R D-R\E\V|A|L 66.7 6 4 3 2 1 unbiased 0.942 60.3 29.88 81.72 0.0 356.8768 1068.616 -0.0002 -0.2 -0.30000000000000004 6.07 11644.1 nuORFdb_human ENST00000596657.1_1_19:6373528-6374597:+ ENST00000596657.1_1_19:6373528-6374597:+|ENST00000245812.7_1_19:6372800-6374597:+ ALKBH7 5' Overlap uORF 5' Overlap uORF GN=ALKBH7 5' Overlap uORF AL20210827_EV_HLA_PDAC_PANFRO413_2D_50e6_W632_classI_fxn1 Exploris_FAIMS VH20221207_MKP_FAIMS2022E_HLAc1_4e7_PDAC.30525.30643.3 3 1 1 10.26 8.33 2.19 1.93 73.7 8 2 2 9340000.0 Hydrogen 39 (S) 10 LPRELSRETL (R) 10 VEERPRTTLI L/P/R/E/L/S-R\E T|L 80.0 8 2 6 1 1 y-ion bias 0.999 93.5 59.67 120.0 -70.0 405.2337 1213.69 -0.0032 -2.7 -0.10000000000000009 6.14 9898.2 nuORFdb_human ENST00000361138.6_1_9:72873923-72874178:+ ENST00000361138.6_1_9:72873923-72874178:+ SMC5 5' Overlap uORF 5' Overlap uORF GN=SMC5 5' Overlap uORF VH20221207_MKP_FAIMS2022E_HLAc1_4e7_PDAC SCP E_20221201_NO30_400nL_HLAc1_4e7_PDAC_Slot2-6_1_3524.8316.1390.2 2 1 1 9.21 6.34 3.79 2.87 75.7 7 0 2 7230.0 Hydrogen 15 (E) 9 LPRPPGSLK (-) 9 LRPPPAVSK L/P/R/P P\G\S\L\K 77.8 7 4 3 0 0 unbiased 28.730635 0.882859 482.7993 964.594 -0.0031 -3.2 -0.8000000000000003 11.0 2438.8 nuORFdb_human ENST00000598273.5_1_19:48284145-48284407:+ ENST00000598273.5_1_19:48284145-48284407:+ SELENOW ncRNA Retained Intron lncRNA GN=SELENOW ncRNA Retained Intron E_20221201_NO30_400nL_HLAc1_4e7_PDAC_Slot2-6_1_3524 SCP E_20221201_NO30_400nL_HLAc1_4e7_PDAC_Slot2-6_1_3524.10305.1504.2 2 1 1 11.75 10.42 3.14 1.33 76.3 6 1 1 10200.0 Hydrogen 25 (S) 9 LPRQPATSL (M) 9 TAPQPRLSL L/P R\Q\P A\T|S\L 55.6 5 5 2 1 0 b-ion bias 35.601612 0.810777 491.786 982.568 -0.0033 -3.3 -0.8999999999999999 9.75 6800.9 nuORFdb_human ENST00000278671.9_1_11:71809441-71810244:- ENST00000278671.9_1_11:71809441-71810244:- LAMTOR1 Out-of-Frame Out-of-Frame GN=LAMTOR1 Out-of-Frame E_20221201_NO30_400nL_HLAc1_4e7_PDAC_Slot2-6_1_3524 Exploris_FAIMS VH20221207_MKP_FAIMS2022E_HLAc1_4e7_PDAC.27162.27162.2 2 1 1 12.63 8.99 4.57 3.64 81.6 7 1 1 6480000.0 Hydrogen 25 (S) 9 LPRQPATSL (M) 9 TNGHKLLSL L/P/R\Q\P A\T|S|L 77.8 7 5 4 2 0 unbiased 0.887 87.1 54.47 92.08 -50.0 491.7872 982.568 -0.0009 -1.0 1.6 9.75 6800.9 nuORFdb_human ENST00000278671.9_1_11:71809441-71810244:- ENST00000278671.9_1_11:71809441-71810244:- LAMTOR1 Out-of-Frame Out-of-Frame GN=LAMTOR1 Out-of-Frame VH20221207_MKP_FAIMS2022E_HLAc1_4e7_PDAC SCP E_20221201_NO30_400nL_HLAc1_4e7_PDAC_Slot2-6_1_3524.12351.1431.2 2 1 1 9.03 6.63 3.22 2.4 60.9 7 0 0 7780.0 Hydrogen 211 (L) 9 LPSEVCHTL (S) 9 LSEPVCHTL L/P/S/E\V C/H\T\L 77.8 7 3 4 0 0 unbiased Carbamidomethylation C:Carbamidomethylation, 42.668667 0.856919 528.2624 1055.519 -0.0023 -2.2 0.19999999999999973 5.24 28016.0 nuORFdb_human ENST00000557552.1_1_14:105238371-105239528:- ENST00000557552.1_1_14:105238371-105239528:- AKT1 ncRNA Retained Intron lncRNA GN=AKT1 ncRNA Retained Intron E_20221201_NO30_400nL_HLAc1_4e7_PDAC_Slot2-6_1_3524 Exploris_FAIMS VH20221207_MKP_FAIMS2022E_HLAc1_4e7_PDAC.33195.33195.2 2 1 1 11.37 10.17 1.11 1.2 88.2 7 1 1 10200000.0 Hydrogen 26 (M) 9 LPSGERLAM (S) 9 LPSEQRFP L/P|S/G/E/R-L A/M 77.8 7 1 6 1 1 y-ion bias 0.875 85.0 63.62 104.89 -70.0 487.258 973.514 -0.0048 -5.0 -2.4 6.0 5771.3 nuORFdb_human ENST00000400770.6_1_2:242617889-242618051:- ENST00000400770.6_1_2:242617889-242618051:- DTYMK 3' dORF 3' dORF GN=DTYMK 3' dORF VH20221207_MKP_FAIMS2022E_HLAc1_4e7_PDAC Exploris_FAIMS VH20221207_MKP_FAIMS2022E_HLAc1_4e7_PDAC.21969.21969.2 2 1 1 9.4 7.32 3.01 2.07 63.8 6 1 1 2530000.0 M34m Hydrogen 26 (M) 9 LPSGERLAm (S) 9 LPSLARADm L/P|S/G/E R L/A/m 66.7 6 1 6 1 0 y-ion bias m:Oxidized methionine 0.951 91.4 45.82 120.0 -70.0 495.2559 973.514 15.991 -3.9 -1.2999999999999998 6.0 5771.3 nuORFdb_human ENST00000400770.6_1_2:242617889-242618051:- ENST00000400770.6_1_2:242617889-242618051:- DTYMK 3' dORF 3' dORF GN=DTYMK 3' dORF VH20221207_MKP_FAIMS2022E_HLAc1_4e7_PDAC Exploris AL20221201_MKP_PDAC_01.43879.43879.1 1 1 1 14.76 11.55 3.46 3.21 93.8 6 0 0 12400000.0 Hydrogen 83 (H) 9 LPSPSPPWS (Q) 9 VTPPPSSPW L/P|S|P|S P|P/W-S 77.8 7 4 6 4 1 dual b/y bias 0.786 100.0 67.37 100.0 0.0 967.489 967.488 0.0007 0.7 -0.10000000000000009 5.52 11585.2 nuORFdb_human ENST00000591661.1_1_18:47801613-47802282:- ENST00000591661.1_1_18:47801613-47802282:- MBD1 ncRNA Retained Intron lncRNA GN=MBD1 ncRNA Retained Intron AL20221201_MKP_PDAC_01 SCP E_20221201_NO30_400nL_HLAc1_4e7_PDAC_Slot2-6_1_3524.20968.481.1 1 2 1 10.52 8.71 3.02 1.81 79.1 7 0 0 28800.0 Hydrogen 83 (H) 9 LPSPSPPWS (Q) 9 VTPPPSSPW L/P/S|P|S\P|P-W S 77.8 7 4 5 3 1 dual b/y bias 72.435875 1.437537 967.4865 967.488 -0.0014 -1.4 1.0 5.52 11585.2 nuORFdb_human ENST00000591661.1_1_18:47801613-47802282:- ENST00000591661.1_1_18:47801613-47802282:- MBD1 ncRNA Retained Intron lncRNA GN=MBD1 ncRNA Retained Intron E_20221201_NO30_400nL_HLAc1_4e7_PDAC_Slot2-6_1_3524 SCP E_20221201_NO30_400nL_HLAc1_4e7_PDAC_Slot2-6_1_3524.9046.1452.2 2 1 1 12.13 10.16 1.63 1.97 83.2 5 1 1 17800.0 Hydrogen 6 (D) 9 LPSRGTETF (A) 9 LVNVDAETF L/P/S R G T\E|T\F 55.6 5 3 3 1 0 unbiased 31.251421 0.843764 504.2616 1007.516 0.0011 1.1 3.5 6.0 2971.5 nuORFdb_human ENST00000295974.12_1_4:41145041-41145116:- ENST00000295974.12_1_4:41145041-41145116:- APBB2 5' uORF 5' uORF GN=APBB2 5' uORF E_20221201_NO30_400nL_HLAc1_4e7_PDAC_Slot2-6_1_3524 SCP E_20221201_NO30_400nL_HLAc1_4e7_PDAC_Slot2-6_1_3524.29813.390.1 1 2 1 13.62 11.75 2.29 1.88 92.9 7 0 0 58200.0 Hydrogen 22 (L) 9 LPSSLIVLL (F) 9 ISLSPIVLL L/P S\S\L\I\V\L\L 77.8 7 6 1 0 0 b-ion bias 103.002167 1.491041 954.6204 954.623 -0.0034 -3.6 -1.2000000000000002 5.52 3688.3 nuORFdb_human ENST00000216225.8_1_22:41363848-41368538:+ ENST00000216225.8_1_22:41363848-41368538:+ RBX1 3' dORF 3' dORF GN=RBX1 3' dORF E_20221201_NO30_400nL_HLAc1_4e7_PDAC_Slot2-6_1_3524 Eva-50mio-3fr AL20210827_EV_HLA_PDAC_PANFRO413_2D_50e6_W632_classI_fxn1.17025.17025.2 2 1 1 11.67 10.12 1.43 1.56 78.9 6 1 1 7120000.0 Hydrogen 40 (E) 9 LPSSQREVL (-) 9 LPHSVYSLL L/P|S/S/Q R E-V|L 66.7 6 2 5 2 1 y-ion bias 0.924 100.0 32.58 100.0 0.0 514.7902 1028.573 -0.0003 -0.3 -0.4 6.0 4979.5 nuORFdb_human ENST00000474414.5_1_1:32852491-32859819:- ENST00000474414.5_1_1:32852491-32859819:-|ENST00000455895.6_1_1:32852491-32860037:- BSDC1 5' Overlap uORF 5' Overlap uORF GN=BSDC1 5' Overlap uORF AL20210827_EV_HLA_PDAC_PANFRO413_2D_50e6_W632_classI_fxn1 Exploris AL20221201_MKP_PDAC_01.50189.50189.1 1 1 1 8.17 5.59 1.58 2.58 76.3 6 0 0 132000000.0 Hydrogen 69 (C) 9 LPSSVLASI (P) 9 LVSPSLLAS L P\S-S\V\L\A\S I 55.6 5 5 0 0 1 b-ion bias 1.0 0.0 76.6 100.0 0.0 886.5244 886.524 0.0 0.0 -0.8 5.52 16261.5 nuORFdb_human ENST00000479835.1_1_7:75362785-75368238:- ENST00000479835.1_1_7:75362785-75368238:- HIP1 ncRNA Processed Transcript lncRNA GN=HIP1 ncRNA Processed Transcript AL20221201_MKP_PDAC_01 SCP E_20221201_NO30_400nL_HLAc1_4e7_PDAC_Slot2-6_1_3524.17658.1444.2 2 1 1 11.08 7.98 2.13 3.1 70.5 7 1 1 25500.0 Hydrogen 149 (S) 9 LPTSKSWTF (G) 9 LPYTNPSKF L/P/T/S/K/S W\T\F 77.8 7 2 5 0 0 y-ion bias 61.002907 0.848915 533.7806 1066.557 -0.002 -1.9 0.5 8.75 27727.3 nuORFdb_human ENST00000486256.5_1_7:6777424-6791020:+ ENST00000486256.5_1_7:6777424-6791020:+ PMS2CL Pseudogene Pseudogene GN=PMS2CL Pseudogene E_20221201_NO30_400nL_HLAc1_4e7_PDAC_Slot2-6_1_3524 Exploris_FAIMS VH20221207_MKP_FAIMS2022E_HLAc1_4e7_PDAC.45381.45381.2 2 3 1 14.07 7.78 6.72 6.3 88.9 8 1 1 60000000.0 Hydrogen 149 (S) 9 LPTSKSWTF (G) 9 LPYTNPSKF L/P|T/S/K-S-W/T/F 100.0 9 1 6 1 2 y-ion bias 0.965 97.9 81.97 28.86 -70.0 533.7809 1066.557 -0.0023 -2.2 0.3999999999999999 8.75 27727.3 nuORFdb_human ENST00000486256.5_1_7:6777424-6791020:+ ENST00000486256.5_1_7:6777424-6791020:+ PMS2CL Pseudogene Pseudogene GN=PMS2CL Pseudogene VH20221207_MKP_FAIMS2022E_HLAc1_4e7_PDAC SCP E_20221201_NO30_400nL_HLAc1_4e7_PDAC_Slot2-6_1_3524.19201.392.1 1 1 1 10.44 4.69 5.01 5.75 80.7 6 0 0 11500.0 Hydrogen 3 (E) 9 LPTSNLVTY (F) 9 VTGKEPLTY L/P T/S N\L\V\T\Y 55.6 5 4 2 0 0 b-ion bias 66.332787 1.49024 1007.5412 1007.541 -0.0008 -0.8 1.5999999999999999 5.52 5114.9 nuORFdb_human T265047_4:56806716-56814058:- T265047_4:56806716-56814058:- lincRNA lncRNA GN=nan lincRNA E_20221201_NO30_400nL_HLAc1_4e7_PDAC_Slot2-6_1_3524 Exploris_FAIMS VH20221207_MKP_FAIMS2022E_HLAc1_4e7_PDAC.33465.33465.3 3 1 1 9.27 7.79 1.12 1.49 72.2 6 1 1 15300000.0 Hydrogen 6 (S) 11 LPVGWRFEAIL (E) 11 PRLFANSNRLL L/P/V G/W R F E\A\I|L 45.5 5 3 4 1 0 unbiased 0.991 87.4 63.82 44.88 -50.0 434.2528 1300.741 0.0027 2.0 4.6 6.0 3020.5 nuORFdb_human ENST00000523911.5_2_8:87526480-87526558:- ENST00000523911.5_2_8:87526480-87526558:- RMDN1 5' uORF 5' uORF GN=RMDN1 5' uORF VH20221207_MKP_FAIMS2022E_HLAc1_4e7_PDAC Exploris_FAIMS VH20221207_MKP_FAIMS2022E_HLAc1_4e7_PDAC.24390.24390.2 2 1 1 10.11 8.26 2.03 1.85 66.4 6 1 1 5370000.0 Hydrogen 21 (V) 9 LPVRAASGY (R) 9 LPSRAGLGY L/P/V/R A A-S|G|Y 66.7 6 2 5 2 1 y-ion bias 0.893 56.8 49.82 120.0 -70.0 467.2596 933.515 -0.0032 -3.5 -0.8999999999999999 8.75 6222.9 nuORFdb_human ENST00000371743.7_1_20:47895187-47895739:+ ENST00000371743.7_1_20:47895187-47895739:+ ZFAS1 Antisense lncRNA GN=ZFAS1 Antisense VH20221207_MKP_FAIMS2022E_HLAc1_4e7_PDAC Eva-50mio-3fr AL20210827_EV_HLA_PDAC_PANFRO413_2D_50e6_W632_classI_fxn3.33729.33729.2 2 1 1 16.31 10.37 6.23 5.94 90.2 9 1 1 10200000.0 Hydrogen 12 (N) 11 LPVRFQGPASF (I) 11 LPVCRNLLSF L/P|V/R\F Q\G\P-A|S|F 81.8 9 6 5 3 1 unbiased 0.979 100.0 61.78 100.0 0.0 609.8345 1218.663 -0.0012 -1.0 -1.5 9.75 3463.9 smORF_v4|nuORFdb_human SIPA1L3_19:38572039-38572137:+ SIPA1L3_19:38572039-38572137:+|ENST00000222345.10_1_19:38571927-38572134:+ SIPA1L3 uORF 19:38572039-38572137:+ 19 SIPA1L3 5' uORF 5' uORF GN=SIPA1L3 AL20210827_EV_HLA_PDAC_PANFRO413_2D_50e6_W632_classI_fxn3 SCP E_20221201_NO30_400nL_HLAc1_4e7_PDAC_Slot2-6_1_3524.22243.1318.2 2 1 1 12.51 6.51 6.8 6.0 65.9 8 1 1 3530.0 Hydrogen 12 (N) 11 LPVRFQGPASF (I) 11 LNKTDLIPASF L/P/V/R F Q|G|P\A|S\F 72.7 8 5 6 3 0 unbiased 76.845108 0.927582 609.8331 1218.663 -0.0042 -3.5 -1.1 9.75 3463.9 smORF_v4|nuORFdb_human SIPA1L3_19:38572039-38572137:+ SIPA1L3_19:38572039-38572137:+|ENST00000222345.10_1_19:38571927-38572134:+ SIPA1L3 uORF 19:38572039-38572137:+ 19 SIPA1L3 5' uORF 5' uORF GN=SIPA1L3 E_20221201_NO30_400nL_HLAc1_4e7_PDAC_Slot2-6_1_3524 SCP E_20221201_NO30_400nL_HLAc1_4e7_PDAC_Slot2-6_1_3524.14119.438.1 1 1 1 9.71 6.14 4.93 3.57 68.5 6 0 0 7310.0 Hydrogen 96 (G) 10 LPVSSGEPAY (T) 10 VPISSDASPF L/P V|S/S G\E|P-A Y 40.0 4 3 4 2 1 unbiased 48.77673 1.462646 1019.5036 1019.504 -0.0044 -4.3 -1.9 4.0 12379.9 nuORFdb_human ENST00000164227.9_1_19:45252055-45254595:+ ENST00000164227.9_1_19:45252055-45254595:+ BCL3 Out-of-Frame Out-of-Frame GN=BCL3 Out-of-Frame E_20221201_NO30_400nL_HLAc1_4e7_PDAC_Slot2-6_1_3524 SCP E_20221201_NO30_400nL_HLAc1_4e7_PDAC_Slot2-6_1_3524.26537.562.1 1 1 1 9.17 4.92 5.31 4.25 66.9 8 0 0 8740.0 Hydrogen 32 (L) 9 LPWPGAAAL (P) 9 LKEQHGAL L/P/W|P\G\A\A\A\L 100.0 9 6 3 1 0 b-ion bias 91.676407 1.389082 895.5005 895.504 -0.0026 -2.9 -0.5 5.52 13487.4 nuORFdb_human ENST00000323716.7_1_7:102715586-102724129:+ ENST00000323716.7_1_7:102715586-102724129:+|ENST00000425331.5_1_7:102715586-102724141:+|ENST00000306450.5_1_7:102715586-102722332:+ ARMC10 5' Overlap uORF 5' Overlap uORF GN=ARMC10 5' Overlap uORF E_20221201_NO30_400nL_HLAc1_4e7_PDAC_Slot2-6_1_3524 20mio-rep1 E_20221201_NO30_400nL_HLAc1_2e7_directIP_titration_rep1_Slot2-4_1_3515.26588.553.1 1 2 1 10.33 5.98 5.91 4.35 74.3 7 0 0 3360.0 Hydrogen 8 (M) 9 LSAGPFVGW (-) 9 VTATWLGW L/S/A/G/P F\V\G\W 77.8 7 3 4 0 0 unbiased 91.81311 1.394135 933.4805 933.483 -0.0019 -2.0 4.2 5.52 1708.1 nuORFdb_human ENST00000370562.1_1_20:60962923-60962974:+ ENST00000370562.1_1_20:60962923-60962974:+|ENST00000343986.8_1_20:60962923-60963562:+ RPS21 3' dORF 3' dORF GN=RPS21 3' dORF E_20221201_NO30_400nL_HLAc1_2e7_directIP_titration_rep1_Slot2-4_1_3515 20mio-rep2 E_20221201_NO30_400nL_HLAc1_2e7_directIP_titration_rep3_Slot2-4_1_3518.26632.545.1 1 2 1 10.15 6.51 5.16 3.64 77.3 6 0 0 3040.0 Hydrogen 8 (M) 9 LSAGPFVGW (-) 9 VTATWLGW L S/A/G|P F\V\G\W 55.6 5 4 3 1 0 unbiased 91.98394 1.398973 933.4778 933.483 -0.0049 -5.2 1.0999999999999996 5.52 1708.1 nuORFdb_human ENST00000370562.1_1_20:60962923-60962974:+ ENST00000370562.1_1_20:60962923-60962974:+|ENST00000343986.8_1_20:60962923-60963562:+ RPS21 3' dORF 3' dORF GN=RPS21 3' dORF E_20221201_NO30_400nL_HLAc1_2e7_directIP_titration_rep3_Slot2-4_1_3518 20mio-rep3 E_20221201_NO30_400nL_HLAc1_2e7_directIP_titration_rep4_Slot2-4_1_3519.26665.539.1 1 2 1 9.96 8.3 5.14 1.67 79.4 6 0 0 3370.0 Hydrogen 8 (M) 9 LSAGPFVGW (-) 9 VTATWLGW L S/A/G/P F\V\G\W 55.6 5 3 3 0 0 unbiased 92.094353 1.402673 933.482 933.483 -0.0009 -0.9 4.8999999999999995 5.52 1708.1 nuORFdb_human ENST00000370562.1_1_20:60962923-60962974:+ ENST00000370562.1_1_20:60962923-60962974:+|ENST00000343986.8_1_20:60962923-60963562:+ RPS21 3' dORF 3' dORF GN=RPS21 3' dORF E_20221201_NO30_400nL_HLAc1_2e7_directIP_titration_rep4_Slot2-4_1_3519 40mio-rep1 E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep1_Slot1-04_1_3496.26851.530.1 1 2 1 8.84 6.33 5.99 2.5 77.0 6 0 0 10900.0 Hydrogen 8 (M) 9 LSAGPFVGW (-) 9 VTATWLGW L S/A/G/P F\V\G\W 55.6 5 3 3 0 0 unbiased 92.760323 1.408363 933.4803 933.483 -0.0029 -3.1 0.10000000000000009 5.52 1708.1 nuORFdb_human ENST00000370562.1_1_20:60962923-60962974:+ ENST00000370562.1_1_20:60962923-60962974:+|ENST00000343986.8_1_20:60962923-60963562:+ RPS21 3' dORF 3' dORF GN=RPS21 3' dORF E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep1_Slot1-04_1_3496 40mio-rep2 E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep2_Slot2-5_1_3520.26632.537.1 1 1 1 9.13 6.53 5.85 2.59 78.2 6 0 0 3750.0 Hydrogen 8 (M) 9 LSAGPFVGW (-) 9 VTATWLGW L S/A/G/P F\V\G\W 55.6 5 3 3 0 0 unbiased 92.001747 1.404202 933.4803 933.483 -0.0029 -3.1 1.8000000000000003 5.52 1708.1 nuORFdb_human ENST00000370562.1_1_20:60962923-60962974:+ ENST00000370562.1_1_20:60962923-60962974:+|ENST00000343986.8_1_20:60962923-60963562:+ RPS21 3' dORF 3' dORF GN=RPS21 3' dORF E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep2_Slot2-5_1_3520 40mio-rep3 E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep3_Slot2-5_1_3521.26643.538.1 1 3 1 8.11 6.66 3.78 1.45 71.2 6 0 0 11500.0 Hydrogen 8 (M) 9 LSAGPFVGW (-) 9 VTATWLGW L S/A/G/P F\V\G\W 55.6 5 3 3 0 0 unbiased 92.033295 1.403222 933.4785 933.483 -0.0049 -5.2 -0.6000000000000005 5.52 1708.1 nuORFdb_human ENST00000370562.1_1_20:60962923-60962974:+ ENST00000370562.1_1_20:60962923-60962974:+|ENST00000343986.8_1_20:60962923-60963562:+ RPS21 3' dORF 3' dORF GN=RPS21 3' dORF E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep3_Slot2-5_1_3521 40mio-rep4 E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep4_Slot2-5_1_3522.26640.543.1 1 2 1 8.36 6.32 4.86 2.04 71.2 6 0 0 4760.0 Hydrogen 8 (M) 9 LSAGPFVGW (-) 9 VTATWLGW L S/A/G/P F\V\G\W 55.6 5 3 3 0 0 unbiased 92.01561 1.400259 933.4798 933.483 -0.0029 -3.1 1.6 5.52 1708.1 nuORFdb_human ENST00000370562.1_1_20:60962923-60962974:+ ENST00000370562.1_1_20:60962923-60962974:+|ENST00000343986.8_1_20:60962923-60963562:+ RPS21 3' dORF 3' dORF GN=RPS21 3' dORF E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep4_Slot2-5_1_3522 10mio-rep2 E_20221201_NO30_400nL_HLAc1_1e7_directIP_titration_rep2_Slot2-3_1_3513.14001.499.1 1 2 1 10.57 3.17 6.61 7.4 69.6 8 0 0 3770.0 Hydrogen 7 (L) 10 LSASGTASEW (V) 10 ASSGTLASAED L S/A/S/G/T|A\S\E\W 80.0 8 4 5 1 0 unbiased 48.368446 1.426486 1008.4567 1008.463 -0.0033 -3.2 3.5 4.0 3163.6 nuORFdb_human ENST00000533012.1_1_11:118889635-118889891:+ ENST00000533012.1_1_11:118889635-118889891:+ TRAPPC4 Out-of-Frame Out-of-Frame GN=TRAPPC4 Out-of-Frame E_20221201_NO30_400nL_HLAc1_1e7_directIP_titration_rep2_Slot2-3_1_3513 10mio-rep3 E_20221201_NO30_400nL_HLAc1_1e7_directIP_titration_rep3_Slot2-3_1_3514.13954.502.1 1 2 1 8.02 0.14 5.85 7.89 61.0 7 0 0 5750.0 Hydrogen 7 (L) 10 LSASGTASEW (V) 10 SSANVPTEGF L S/A/S G/T|A|S\E\W 60.0 6 4 5 2 0 unbiased 48.208256 1.424647 1008.4579 1008.463 -0.0033 -3.2 3.0999999999999996 4.0 3163.6 nuORFdb_human ENST00000533012.1_1_11:118889635-118889891:+ ENST00000533012.1_1_11:118889635-118889891:+ TRAPPC4 Out-of-Frame Out-of-Frame GN=TRAPPC4 Out-of-Frame E_20221201_NO30_400nL_HLAc1_1e7_directIP_titration_rep3_Slot2-3_1_3514 20mio-rep3 E_20221201_NO30_400nL_HLAc1_2e7_directIP_titration_rep4_Slot2-4_1_3519.13949.495.1 1 1 1 9.22 1.99 7.08 7.23 68.4 8 0 0 3930.0 Hydrogen 7 (L) 10 LSASGTASEW (V) 10 ASSGTLASAED L S/A/S/G/T|A\S\E\W 80.0 8 4 5 1 0 unbiased 48.181931 1.428818 1008.46 1008.463 -0.0033 -3.2 2.5999999999999996 4.0 3163.6 nuORFdb_human ENST00000533012.1_1_11:118889635-118889891:+ ENST00000533012.1_1_11:118889635-118889891:+ TRAPPC4 Out-of-Frame Out-of-Frame GN=TRAPPC4 Out-of-Frame E_20221201_NO30_400nL_HLAc1_2e7_directIP_titration_rep4_Slot2-4_1_3519 40mio-rep1 E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep1_Slot1-04_1_3496.14216.471.1 1 1 1 9.42 0.74 7.86 8.68 74.3 8 0 0 7700.0 Hydrogen 7 (L) 10 LSASGTASEW (V) 10 IASTSGASNNS L S/A/S/G/T\A\S\E\W 80.0 8 4 4 0 0 unbiased 49.106361 1.443469 1008.4571 1008.463 -0.0033 -3.2 0.0 4.0 3163.6 nuORFdb_human ENST00000533012.1_1_11:118889635-118889891:+ ENST00000533012.1_1_11:118889635-118889891:+ TRAPPC4 Out-of-Frame Out-of-Frame GN=TRAPPC4 Out-of-Frame E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep1_Slot1-04_1_3496 40mio-rep2 E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep2_Slot2-5_1_3520.13907.486.1 1 1 1 7.96 1.99 5.07 5.97 68.7 8 0 0 9430.0 Hydrogen 7 (L) 10 LSASGTASEW (V) 10 ASSGTLASAED L S/A/S/G/T\A\S\E\W 80.0 8 4 4 0 0 unbiased 48.040604 1.434561 1008.4588 1008.463 -0.0033 -3.2 1.7000000000000002 4.0 3163.6 nuORFdb_human ENST00000533012.1_1_11:118889635-118889891:+ ENST00000533012.1_1_11:118889635-118889891:+ TRAPPC4 Out-of-Frame Out-of-Frame GN=TRAPPC4 Out-of-Frame E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep2_Slot2-5_1_3520 40mio-rep3 E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep3_Slot2-5_1_3521.13909.488.1 1 2 1 13.61 2.92 10.59 10.68 83.8 8 0 0 10400.0 Hydrogen 7 (L) 10 LSASGTASEW (V) 10 IASTSGASNNS L S/A/S/G/T|A\S\E\W 80.0 8 4 5 1 0 unbiased 48.051262 1.433003 1008.4595 1008.463 -0.0033 -3.2 1.3999999999999995 4.0 3163.6 nuORFdb_human ENST00000533012.1_1_11:118889635-118889891:+ ENST00000533012.1_1_11:118889635-118889891:+ TRAPPC4 Out-of-Frame Out-of-Frame GN=TRAPPC4 Out-of-Frame E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep3_Slot2-5_1_3521 40mio-rep4 E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep4_Slot2-5_1_3522.13898.485.1 1 1 1 7.73 2.81 5.75 4.92 63.0 7 0 0 7860.0 Hydrogen 7 (L) 10 LSASGTASEW (V) 10 ASSGTLASAED L S/A/S/G T\A\S|E\W 60.0 6 4 4 1 0 unbiased 47.996696 1.434766 1008.4569 1008.463 -0.0033 -3.2 1.5 4.0 3163.6 nuORFdb_human ENST00000533012.1_1_11:118889635-118889891:+ ENST00000533012.1_1_11:118889635-118889891:+ TRAPPC4 Out-of-Frame Out-of-Frame GN=TRAPPC4 Out-of-Frame E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep4_Slot2-5_1_3522 40mio-rep1 E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep1_Slot1-04_1_3496.10725.1490.2 2 1 1 8.89 6.49 3.9 2.4 79.9 6 1 1 7890.0 Hydrogen 126 (N) 8 LSATRQVF (L) 8 LSRATQVF L/S/A/T/R Q\V\F 75.0 6 2 4 0 0 y-ion bias 37.050163 0.819988 461.2604 921.515 -0.0025 -2.7 0.5 9.75 18481.0 nuORFdb_human ENST00000313478.11_1_15:50941001-50979001:- ENST00000313478.11_1_15:50941001-50979001:- TRPM7 5' Overlap uORF 5' Overlap uORF GN=TRPM7 5' Overlap uORF E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep1_Slot1-04_1_3496 40mio-rep2 E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep2_Slot2-5_1_3520.10428.1507.2 2 1 1 8.43 5.91 4.11 2.51 69.9 6 1 1 8570.0 Hydrogen 126 (N) 8 LSATRQVF (L) 8 LSRATQVF L/S/A/T/R Q\V\F 75.0 6 2 4 0 0 y-ion bias 36.025291 0.808963 461.2583 921.515 -0.0065 -7.1 -2.1999999999999993 9.75 18481.0 nuORFdb_human ENST00000313478.11_1_15:50941001-50979001:- ENST00000313478.11_1_15:50941001-50979001:- TRPM7 5' Overlap uORF 5' Overlap uORF GN=TRPM7 5' Overlap uORF E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep2_Slot2-5_1_3520 40mio-rep3 E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep3_Slot2-5_1_3521.10429.1500.2 2 2 1 10.6 7.86 5.32 2.74 76.3 7 1 1 15100.0 Hydrogen 126 (N) 8 LSATRQVF (L) 8 LSRATQVF L/S/A/T/R\Q\V\F 100.0 8 3 4 0 0 unbiased 36.031303 0.813278 461.2585 921.515 -0.0065 -7.1 -2.5 9.75 18481.0 nuORFdb_human ENST00000313478.11_1_15:50941001-50979001:- ENST00000313478.11_1_15:50941001-50979001:- TRPM7 5' Overlap uORF 5' Overlap uORF GN=TRPM7 5' Overlap uORF E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep3_Slot2-5_1_3521 40mio-rep4 E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep4_Slot2-5_1_3522.10445.1498.2 2 1 1 11.79 9.43 4.72 2.36 84.3 7 1 1 8540.0 Hydrogen 126 (N) 8 LSATRQVF (L) 8 LSRATQVF L/S/A/T/R\Q\V\F 100.0 8 3 4 0 0 unbiased 36.072399 0.814518 461.2586 921.515 -0.0045 -4.9 -0.20000000000000018 9.75 18481.0 nuORFdb_human ENST00000313478.11_1_15:50941001-50979001:- ENST00000313478.11_1_15:50941001-50979001:- TRPM7 5' Overlap uORF 5' Overlap uORF GN=TRPM7 5' Overlap uORF E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep4_Slot2-5_1_3522 40mio-rep1 E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep1_Slot1-04_1_3496.17266.1390.2 2 3 1 11.86 4.51 9.2 7.35 64.3 8 0 0 17000.0 M29m Hydrogen 26 (K) 9 LSDmFLSHY (L) 9 LSDmQVCLF L/S/D|m|F|L|S\H\Y 100.0 9 6 6 4 0 dual b/y bias m:Oxidized methionine 59.641411 0.882394 564.7541 1112.508 15.9926 -2.0 1.2000000000000002 5.08 5415.2 nuORFdb_human ENST00000584471.5_1_17:65375300-65375444:+ ENST00000584471.5_1_17:65375300-65375444:+ PITPNC1 5' uORF 5' uORF GN=PITPNC1 5' uORF E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep1_Slot1-04_1_3496 20mio-rep1 E_20221201_NO30_400nL_HLAc1_2e7_directIP_titration_rep1_Slot2-4_1_3515.14164.1438.2 2 1 1 9.97 3.25 6.72 6.72 68.0 8 1 1 6470.0 Hydrogen 3 (E) 9 LSDVLVKQY (S) 9 LSDVLQYVK L/S/D|V|L/V|K\Q\Y 100.0 9 5 6 3 0 dual b/y bias 48.920467 0.85258 532.7981 1064.599 -0.0099 -9.3 -3.1000000000000005 5.83 6171.9 nuORFdb_human ENST00000380359.3_1_10:5493865-5494450:+ ENST00000380359.3_1_10:5493865-5494450:+ NET1 Out-of-Frame Out-of-Frame GN=NET1 Out-of-Frame E_20221201_NO30_400nL_HLAc1_2e7_directIP_titration_rep1_Slot2-4_1_3515 20mio-rep2 E_20221201_NO30_400nL_HLAc1_2e7_directIP_titration_rep3_Slot2-4_1_3518.14239.1426.2 2 1 1 7.24 7.24 7.24 61.9 7 1 1 3150.0 Hydrogen 3 (E) 9 LSDVLVKQY (S) 9 L/S/D V|L/V|K|Q-Y 77.8 7 3 6 3 1 dual b/y bias 49.183998 0.860311 532.799 1064.599 -0.0079 -7.4 -1.1000000000000005 5.83 6171.9 nuORFdb_human ENST00000380359.3_1_10:5493865-5494450:+ ENST00000380359.3_1_10:5493865-5494450:+ NET1 Out-of-Frame Out-of-Frame GN=NET1 Out-of-Frame E_20221201_NO30_400nL_HLAc1_2e7_directIP_titration_rep3_Slot2-4_1_3518 20mio-rep3 E_20221201_NO30_400nL_HLAc1_2e7_directIP_titration_rep4_Slot2-4_1_3519.14202.1430.2 2 1 1 7.13 7.13 7.13 60.9 7 1 1 5660.0 Hydrogen 3 (E) 9 LSDVLVKQY (S) 9 L/S/D\V|L/V|K Q\Y 77.8 7 4 5 2 0 unbiased 49.055168 0.85738 532.7994 1064.599 -0.0079 -7.4 -1.6000000000000005 5.83 6171.9 nuORFdb_human ENST00000380359.3_1_10:5493865-5494450:+ ENST00000380359.3_1_10:5493865-5494450:+ NET1 Out-of-Frame Out-of-Frame GN=NET1 Out-of-Frame E_20221201_NO30_400nL_HLAc1_2e7_directIP_titration_rep4_Slot2-4_1_3519 40mio-rep1 E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep1_Slot1-04_1_3496.14461.1402.2 2 1 1 13.47 7.09 7.0 6.38 79.1 8 1 1 13000.0 Hydrogen 3 (E) 9 LSDVLVKQY (S) 9 LSDVLQYVK L/S/D|V|L/V|K|Q\Y 100.0 9 5 7 4 0 dual b/y bias 49.952915 0.874816 532.8012 1064.599 -0.0039 -3.7 -0.5 5.83 6171.9 nuORFdb_human ENST00000380359.3_1_10:5493865-5494450:+ ENST00000380359.3_1_10:5493865-5494450:+ NET1 Out-of-Frame Out-of-Frame GN=NET1 Out-of-Frame E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep1_Slot1-04_1_3496 40mio-rep2 E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep2_Slot2-5_1_3520.14151.1415.2 2 1 1 11.14 4.33 6.81 6.81 69.8 8 1 1 12900.0 Hydrogen 3 (E) 9 LSDVLVKQY (S) 9 LSDVLQYVK L/S/D|V|L/V|K\Q\Y 100.0 9 5 6 3 0 dual b/y bias 48.883686 0.866866 532.8017 1064.599 -0.0019 -1.8 3.1000000000000005 5.83 6171.9 nuORFdb_human ENST00000380359.3_1_10:5493865-5494450:+ ENST00000380359.3_1_10:5493865-5494450:+ NET1 Out-of-Frame Out-of-Frame GN=NET1 Out-of-Frame E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep2_Slot2-5_1_3520 40mio-rep3 E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep3_Slot2-5_1_3521.14164.1420.2 2 1 1 12.22 5.31 6.91 6.91 71.3 8 1 1 12500.0 Hydrogen 3 (E) 9 LSDVLVKQY (S) 9 LSDVLQYVK L/S/D|V|L/V|K/Q\Y 100.0 9 4 7 3 0 dual b/y bias 48.931435 0.863884 532.8008 1064.599 -0.0039 -3.7 0.8999999999999995 5.83 6171.9 nuORFdb_human ENST00000380359.3_1_10:5493865-5494450:+ ENST00000380359.3_1_10:5493865-5494450:+ NET1 Out-of-Frame Out-of-Frame GN=NET1 Out-of-Frame E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep3_Slot2-5_1_3521 40mio-rep4 E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep4_Slot2-5_1_3522.14146.1424.2 2 1 1 11.87 3.74 8.13 8.13 75.1 8 1 1 7070.0 Hydrogen 3 (E) 9 LSDVLVKQY (S) 9 LSDVLQYVK L/S/D|V|L/V|K|Q\Y 100.0 9 5 7 4 0 dual b/y bias 48.855667 0.860998 532.8006 1064.599 -0.0039 -3.7 1.0 5.83 6171.9 nuORFdb_human ENST00000380359.3_1_10:5493865-5494450:+ ENST00000380359.3_1_10:5493865-5494450:+ NET1 Out-of-Frame Out-of-Frame GN=NET1 Out-of-Frame E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep4_Slot2-5_1_3522 10mio-rep2 E_20221201_NO30_400nL_HLAc1_1e7_directIP_titration_rep2_Slot2-3_1_3513.21871.766.1 1 1 1 9.74 7.52 2.23 2.21 77.3 5 0 0 11400.0 Hydrogen 1 (-) 8 LSGAVVGL (S) 8 GLGTVVGL L S G\A|V|V\G\L 62.5 5 5 2 2 0 b-ion bias 75.544228 1.266151 715.4287 715.435 -0.0059 -8.2 -1.4999999999999991 5.52 4116.6 nuORFdb_human ENST00000595090.5_1_19:49496905-49497022:+ ENST00000595090.5_1_19:49496905-49497022:+ RUVBL2 5' uORF 5' uORF GN=RUVBL2 5' uORF E_20221201_NO30_400nL_HLAc1_1e7_directIP_titration_rep2_Slot2-3_1_3513 20mio-rep2 E_20221201_NO30_400nL_HLAc1_2e7_directIP_titration_rep3_Slot2-4_1_3518.21851.761.1 1 1 1 8.65 6.99 1.63 1.65 75.6 5 0 0 4710.0 Hydrogen 1 (-) 8 LSGAVVGL (S) 8 GLGTVVGL L S G\A\V\V\G\L 62.5 5 5 0 0 0 b-ion bias 75.475922 1.269449 715.4284 715.435 -0.0069 -9.6 -3.3 5.52 4116.6 nuORFdb_human ENST00000595090.5_1_19:49496905-49497022:+ ENST00000595090.5_1_19:49496905-49497022:+ RUVBL2 5' uORF 5' uORF GN=RUVBL2 5' uORF E_20221201_NO30_400nL_HLAc1_2e7_directIP_titration_rep3_Slot2-4_1_3518 10mio-rep1 E_20221201_NO30_400nL_HLAc1_1e7_directIP_titration_rep1_Slot2-3_1_3512.25455.376.1 1 1 1 11.69 7.88 4.86 3.81 78.5 7 0 0 1200.0 Hydrogen 28 (L) 10 LSLPSAPATW (I) 10 LASPTVPATW L S/L/P S/A|P\A|T\W 60.0 6 4 5 2 0 unbiased 87.924812 1.499765 1042.5524 1042.557 -0.0068 -6.5 1.2000000000000002 5.52 5827.4 nuORFdb_human ENST00000336596.6_1_3:89156791-89156962:+ ENST00000336596.6_1_3:89156791-89156962:+ EPHA3 5' Overlap uORF 5' Overlap uORF GN=EPHA3 5' Overlap uORF E_20221201_NO30_400nL_HLAc1_1e7_directIP_titration_rep1_Slot2-3_1_3512 20mio-rep1 E_20221201_NO30_400nL_HLAc1_2e7_directIP_titration_rep1_Slot2-4_1_3515.25354.384.1 1 3 1 13.73 7.8 6.63 5.93 88.5 7 0 0 7450.0 Hydrogen 28 (L) 10 LSLPSAPATW (I) 10 LASPTVPATW L S/L/P S/A|P\A|T\W 60.0 6 4 5 2 0 unbiased 87.552452 1.494935 1042.5451 1042.557 -0.0068 -6.5 -0.2999999999999998 5.52 5827.4 nuORFdb_human ENST00000336596.6_1_3:89156791-89156962:+ ENST00000336596.6_1_3:89156791-89156962:+ EPHA3 5' Overlap uORF 5' Overlap uORF GN=EPHA3 5' Overlap uORF E_20221201_NO30_400nL_HLAc1_2e7_directIP_titration_rep1_Slot2-4_1_3515 20mio-rep2 E_20221201_NO30_400nL_HLAc1_2e7_directIP_titration_rep3_Slot2-4_1_3518.25389.372.1 1 3 1 12.28 5.99 6.1 6.29 84.5 6 0 0 5750.0 Hydrogen 28 (L) 10 LSLPSAPATW (I) 10 LASPTVPATW L S/L/P S/A|P A|T\W 40.0 4 3 5 2 0 unbiased 87.692825 1.501764 1042.5513 1042.557 -0.0068 -6.5 -0.20000000000000018 5.52 5827.4 nuORFdb_human ENST00000336596.6_1_3:89156791-89156962:+ ENST00000336596.6_1_3:89156791-89156962:+ EPHA3 5' Overlap uORF 5' Overlap uORF GN=EPHA3 5' Overlap uORF E_20221201_NO30_400nL_HLAc1_2e7_directIP_titration_rep3_Slot2-4_1_3518 20mio-rep3 E_20221201_NO30_400nL_HLAc1_2e7_directIP_titration_rep4_Slot2-4_1_3519.25422.374.1 1 3 1 13.7 7.88 6.52 5.82 89.9 7 0 0 7020.0 Hydrogen 28 (L) 10 LSLPSAPATW (I) 10 LASPTVPATW L S/L/P S/A|P-A|T\W 60.0 6 3 5 2 1 unbiased 87.79747 1.500501 1042.5522 1042.557 -0.0068 -6.5 -0.7000000000000002 5.52 5827.4 nuORFdb_human ENST00000336596.6_1_3:89156791-89156962:+ ENST00000336596.6_1_3:89156791-89156962:+ EPHA3 5' Overlap uORF 5' Overlap uORF GN=EPHA3 5' Overlap uORF E_20221201_NO30_400nL_HLAc1_2e7_directIP_titration_rep4_Slot2-4_1_3519 40mio-rep1 E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep1_Slot1-04_1_3496.25610.347.1 1 2 1 14.8 9.64 7.1 5.17 91.7 7 0 0 35400.0 Hydrogen 28 (L) 10 LSLPSAPATW (I) 10 LASPTVPATW L S/L/P S/A|P/A|T\W 60.0 6 3 6 2 0 y-ion bias 88.472115 1.516565 1042.552 1042.557 -0.0068 -6.5 -3.3 5.52 5827.4 nuORFdb_human ENST00000336596.6_1_3:89156791-89156962:+ ENST00000336596.6_1_3:89156791-89156962:+ EPHA3 5' Overlap uORF 5' Overlap uORF GN=EPHA3 5' Overlap uORF E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep1_Slot1-04_1_3496 40mio-rep2 E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep2_Slot2-5_1_3520.25394.1487.2 2 4 1 13.65 5.45 7.58 8.19 86.0 6 0 0 17800.0 Hydrogen 28 (L) 10 LSLPSAPATW (I) 10 LASPTVPATW L S/L|P S/A|P A|T\W 40.0 4 4 5 3 0 unbiased 87.72245 0.821987 521.7789 1042.557 -0.006 -5.8 -0.8999999999999995 5.52 5827.4 nuORFdb_human ENST00000336596.6_1_3:89156791-89156962:+ ENST00000336596.6_1_3:89156791-89156962:+ EPHA3 5' Overlap uORF 5' Overlap uORF GN=EPHA3 5' Overlap uORF E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep2_Slot2-5_1_3520 40mio-rep3 E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep3_Slot2-5_1_3521.25426.1506.2 2 6 1 15.88 8.38 8.5 7.51 89.7 6 0 0 25700.0 Hydrogen 28 (L) 10 LSLPSAPATW (I) 10 LASPTVPATW L S L|P S/A|P/A|T\W 50.0 5 4 5 3 0 unbiased 87.830971 0.809788 521.7777 1042.557 -0.008 -7.7 -3.1000000000000005 5.52 5827.4 nuORFdb_human ENST00000336596.6_1_3:89156791-89156962:+ ENST00000336596.6_1_3:89156791-89156962:+ EPHA3 5' Overlap uORF 5' Overlap uORF GN=EPHA3 5' Overlap uORF E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep3_Slot2-5_1_3521 40mio-rep4 E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep4_Slot2-5_1_3522.25414.1508.2 2 7 1 12.56 5.34 7.86 7.22 83.5 6 0 0 36600.0 Hydrogen 28 (L) 10 LSLPSAPATW (I) 10 LLSPKPDSW L S L|P|S/A|P A|T\W 50.0 5 5 5 4 0 dual b/y bias 87.7799 0.808701 521.7781 1042.557 -0.008 -7.7 -3.0 5.52 5827.4 nuORFdb_human ENST00000336596.6_1_3:89156791-89156962:+ ENST00000336596.6_1_3:89156791-89156962:+ EPHA3 5' Overlap uORF 5' Overlap uORF GN=EPHA3 5' Overlap uORF E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep4_Slot2-5_1_3522 40mio-rep1 E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep1_Slot1-04_1_3496.21724.1390.2 2 1 1 9.06 4.44 3.96 4.62 57.4 8 1 1 6540.0 Hydrogen 6 (W) 10 LSLRLSGSDW (R) 10 LERMRKDW L/S/L R\L\S\G\S|D|W 80.0 8 6 4 2 0 unbiased 75.045631 0.882512 567.3 1133.595 -0.0022 -2.0 1.2000000000000002 5.84 5860.6 nuORFdb_human ENST00000309061.11_1_19:5790104-5790257:- ENST00000309061.11_1_19:5790104-5790257:- DUS3L Out-of-Frame Out-of-Frame GN=DUS3L Out-of-Frame E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep1_Slot1-04_1_3496 40mio-rep1 E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep1_Slot1-04_1_3496.26212.1372.2 2 2 1 15.86 8.61 5.44 7.25 87.7 8 0 0 12100.0 Hydrogen 10 (T) 9 LSMSELHLW (A) 9 LSQDPRnVW L/S/M|S|E/L/H\L\W 100.0 9 4 6 2 0 unbiased 90.553497 0.893934 558.2797 1115.555 -0.0027 -2.4 0.8000000000000003 5.24 6120.8 nuORFdb_human ENST00000500337.6_1_5:43454122-43476351:- ENST00000500337.6_1_5:43454122-43476351:- TMEM267 5' uORF 5' uORF GN=TMEM267 5' uORF E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep1_Slot1-04_1_3496 1mio-rep1 E_20221201_NO30_400nL_HLAc1_1e6_directIP_titration_rep1_Slot1-06_1_3498.13099.1481.2 2 1 1 12.54 5.43 4.74 7.1 76.4 7 0 1 4550.0 Hydrogen 9 (H) 9 LSRPPLSTM (F) 9 KKIFFSTM L/S/R|P/P L|S|T\M 77.8 7 4 6 3 0 dual b/y bias 45.245991 0.825761 501.2721 1001.545 -0.0081 -8.1 -3.1999999999999993 9.75 4005.7 nuORFdb_human ENST00000640129.1_1_12:9723456-9724120:+ ENST00000640129.1_1_12:9723456-9724120:+ RP11-726G1.1 Out-of-Frame Out-of-Frame GN=RP11-726G1.1 Out-of-Frame E_20221201_NO30_400nL_HLAc1_1e6_directIP_titration_rep1_Slot1-06_1_3498 10mio-rep2 E_20221201_NO30_400nL_HLAc1_1e7_directIP_titration_rep2_Slot2-3_1_3513.12721.1472.2 2 1 1 11.58 5.55 4.93 6.04 76.2 6 0 1 6420.0 Hydrogen 9 (H) 9 LSRPPLSTM (F) 9 SRPLPSLTM L S/R|P\P L|S|T\M 55.6 5 5 4 3 0 dual b/y bias 43.94833 0.83124 501.2721 1001.545 -0.0081 -8.1 -1.3999999999999995 9.75 4005.7 nuORFdb_human ENST00000640129.1_1_12:9723456-9724120:+ ENST00000640129.1_1_12:9723456-9724120:+ RP11-726G1.1 Out-of-Frame Out-of-Frame GN=RP11-726G1.1 Out-of-Frame E_20221201_NO30_400nL_HLAc1_1e7_directIP_titration_rep2_Slot2-3_1_3513 20mio-rep2 E_20221201_NO30_400nL_HLAc1_2e7_directIP_titration_rep3_Slot2-4_1_3518.12762.1473.2 2 1 1 10.56 4.08 4.41 6.48 77.7 6 0 1 17300.0 Hydrogen 9 (H) 9 LSRPPLSTM (F) 9 SRPLPSLTM L/S/R|P P L|S|T\M 66.7 6 4 5 3 0 dual b/y bias 44.081165 0.830224 501.2726 1001.545 -0.0061 -6.1 0.20000000000000018 9.75 4005.7 nuORFdb_human ENST00000640129.1_1_12:9723456-9724120:+ ENST00000640129.1_1_12:9723456-9724120:+ RP11-726G1.1 Out-of-Frame Out-of-Frame GN=RP11-726G1.1 Out-of-Frame E_20221201_NO30_400nL_HLAc1_2e7_directIP_titration_rep3_Slot2-4_1_3518 20mio-rep3 E_20221201_NO30_400nL_HLAc1_2e7_directIP_titration_rep4_Slot2-4_1_3519.12684.1470.2 2 2 1 15.04 7.05 7.03 7.99 83.8 7 0 1 14400.0 Hydrogen 9 (H) 9 LSRPPLSTM (F) 9 SRPLPSLTM L/S/R|P\P L|S|T\M 77.8 7 5 5 3 0 dual b/y bias 43.815109 0.832178 501.273 1001.545 -0.0061 -6.1 -0.2999999999999998 9.75 4005.7 nuORFdb_human ENST00000640129.1_1_12:9723456-9724120:+ ENST00000640129.1_1_12:9723456-9724120:+ RP11-726G1.1 Out-of-Frame Out-of-Frame GN=RP11-726G1.1 Out-of-Frame E_20221201_NO30_400nL_HLAc1_2e7_directIP_titration_rep4_Slot2-4_1_3519 40mio-rep1 E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep1_Slot1-04_1_3496.13004.1455.2 2 2 1 15.82 8.54 4.85 7.28 89.6 7 0 1 105000.0 Hydrogen 9 (H) 9 LSRPPLSTM (F) 9 KKIFFSTM L/S/R|P\P L|S|T\M 77.8 7 5 5 3 0 dual b/y bias 44.920578 0.842121 501.2742 1001.545 -0.0041 -4.1 -0.8999999999999995 9.75 4005.7 nuORFdb_human ENST00000640129.1_1_12:9723456-9724120:+ ENST00000640129.1_1_12:9723456-9724120:+ RP11-726G1.1 Out-of-Frame Out-of-Frame GN=RP11-726G1.1 Out-of-Frame E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep1_Slot1-04_1_3496 40mio-rep2 E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep2_Slot2-5_1_3520.12674.1470.2 2 2 1 14.27 8.13 3.87 6.14 88.1 6 0 1 94600.0 Hydrogen 9 (H) 9 LSRPPLSTM (F) 9 KKIFFSTM L/S/R|P P L|S|T\M 66.7 6 4 5 3 0 dual b/y bias 43.782692 0.832478 501.2735 1001.545 -0.0041 -4.1 0.8000000000000007 9.75 4005.7 nuORFdb_human ENST00000640129.1_1_12:9723456-9724120:+ ENST00000640129.1_1_12:9723456-9724120:+ RP11-726G1.1 Out-of-Frame Out-of-Frame GN=RP11-726G1.1 Out-of-Frame E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep2_Slot2-5_1_3520 40mio-rep3 E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep3_Slot2-5_1_3521.12675.1474.2 2 2 1 15.07 8.8 4.34 6.27 86.6 8 0 1 76600.0 Hydrogen 9 (H) 9 LSRPPLSTM (F) 9 SRPLPSLTM L/S/R|P\P\L|S|T\M 100.0 9 6 5 3 0 dual b/y bias 43.789909 0.830018 501.2737 1001.545 -0.0041 -4.1 0.5 9.75 4005.7 nuORFdb_human ENST00000640129.1_1_12:9723456-9724120:+ ENST00000640129.1_1_12:9723456-9724120:+ RP11-726G1.1 Out-of-Frame Out-of-Frame GN=RP11-726G1.1 Out-of-Frame E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep3_Slot2-5_1_3521 40mio-rep4 E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep4_Slot2-5_1_3522.12675.1471.2 2 2 1 16.93 9.74 6.64 7.19 91.4 8 0 1 70600.0 Hydrogen 9 (H) 9 LSRPPLSTM (F) 9 SRPLPSLTM L/S/R|P\P\L|S|T\M 100.0 9 6 5 3 0 dual b/y bias 43.770996 0.831703 501.2735 1001.545 -0.0061 -6.1 -1.3999999999999995 9.75 4005.7 nuORFdb_human ENST00000640129.1_1_12:9723456-9724120:+ ENST00000640129.1_1_12:9723456-9724120:+ RP11-726G1.1 Out-of-Frame Out-of-Frame GN=RP11-726G1.1 Out-of-Frame E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep4_Slot2-5_1_3522 5mio-rep1 E_20221201_NO30_400nL_HLAc1_5e6_directIP_titration_rep1_Slot1-37_1_3502.13014.1466.2 2 1 1 9.84 4.48 3.41 5.36 71.4 7 0 1 4940.0 Hydrogen 9 (H) 9 LSRPPLSTM (F) 9 SRPLPSLTM L/S/R/P\P L\S|T\M 77.8 7 4 4 1 0 unbiased 44.954082 0.835034 501.2744 1001.545 -0.0041 -4.1 2.9000000000000004 9.75 4005.7 nuORFdb_human ENST00000640129.1_1_12:9723456-9724120:+ ENST00000640129.1_1_12:9723456-9724120:+ RP11-726G1.1 Out-of-Frame Out-of-Frame GN=RP11-726G1.1 Out-of-Frame E_20221201_NO30_400nL_HLAc1_5e6_directIP_titration_rep1_Slot1-37_1_3502 10mio-rep1 E_20221201_NO30_400nL_HLAc1_1e7_directIP_titration_rep1_Slot2-3_1_3512.9255.1497.2 2 3 1 10.03 3.78 4.49 6.25 81.0 6 0 1 55700.0 M17m Hydrogen 9 (H) 9 LSRPPLSTm (F) 9 ISRTSPLPm L/S/R|P P L|S|T\m 66.7 6 4 5 3 0 dual b/y bias m:Oxidized methionine 31.975733 0.815199 509.2702 1001.545 15.9879 -6.9 0.7999999999999998 9.75 4005.7 nuORFdb_human ENST00000640129.1_1_12:9723456-9724120:+ ENST00000640129.1_1_12:9723456-9724120:+ RP11-726G1.1 Out-of-Frame Out-of-Frame GN=RP11-726G1.1 Out-of-Frame E_20221201_NO30_400nL_HLAc1_1e7_directIP_titration_rep1_Slot2-3_1_3512 10mio-rep2 E_20221201_NO30_400nL_HLAc1_1e7_directIP_titration_rep2_Slot2-3_1_3513.9198.1477.2 2 3 1 10.95 4.23 4.94 6.72 71.4 7 0 1 50100.0 M17m Hydrogen 9 (H) 9 LSRPPLSTm (F) 9 SRPLPSLTm L/S/R|P/P L\S|T\m 77.8 7 4 5 2 0 unbiased m:Oxidized methionine 31.778839 0.827817 509.2702 1001.545 15.9879 -6.9 -0.20000000000000018 9.75 4005.7 nuORFdb_human ENST00000640129.1_1_12:9723456-9724120:+ ENST00000640129.1_1_12:9723456-9724120:+ RP11-726G1.1 Out-of-Frame Out-of-Frame GN=RP11-726G1.1 Out-of-Frame E_20221201_NO30_400nL_HLAc1_1e7_directIP_titration_rep2_Slot2-3_1_3513 10mio-rep3 E_20221201_NO30_400nL_HLAc1_1e7_directIP_titration_rep3_Slot2-3_1_3514.9101.1482.2 2 3 1 12.25 3.62 6.06 8.63 68.8 6 0 1 50000.0 M17m Hydrogen 9 (H) 9 LSRPPLSTm (F) 9 SRPLPSLTm L/S/R|P P L|S\T\m 66.7 6 4 4 2 0 unbiased m:Oxidized methionine 31.446527 0.825079 509.2699 1001.545 15.9879 -6.9 -0.6000000000000005 9.75 4005.7 nuORFdb_human ENST00000640129.1_1_12:9723456-9724120:+ ENST00000640129.1_1_12:9723456-9724120:+ RP11-726G1.1 Out-of-Frame Out-of-Frame GN=RP11-726G1.1 Out-of-Frame E_20221201_NO30_400nL_HLAc1_1e7_directIP_titration_rep3_Slot2-3_1_3514 20mio-rep1 E_20221201_NO30_400nL_HLAc1_2e7_directIP_titration_rep1_Slot2-4_1_3515.9121.1464.2 2 3 1 13.03 5.56 5.14 7.47 87.0 6 0 1 134000.0 M17m Hydrogen 9 (H) 9 LSRPPLSTm (F) 9 ISRTSPLPm L/S/R|P P L|S|T\m 66.7 6 4 5 3 0 dual b/y bias m:Oxidized methionine 31.50709 0.836056 509.2702 1001.545 15.9879 -6.9 -0.7000000000000002 9.75 4005.7 nuORFdb_human ENST00000640129.1_1_12:9723456-9724120:+ ENST00000640129.1_1_12:9723456-9724120:+ RP11-726G1.1 Out-of-Frame Out-of-Frame GN=RP11-726G1.1 Out-of-Frame E_20221201_NO30_400nL_HLAc1_2e7_directIP_titration_rep1_Slot2-4_1_3515 20mio-rep2 E_20221201_NO30_400nL_HLAc1_2e7_directIP_titration_rep3_Slot2-4_1_3518.9187.1471.2 2 4 1 15.36 8.5 4.66 6.86 83.1 7 0 1 168000.0 M17m Hydrogen 9 (H) 9 LSRPPLSTm (F) 9 KKIFFSTm L/S/R|P/P L|S|T\m 77.8 7 4 6 3 0 dual b/y bias m:Oxidized methionine 31.736509 0.831607 509.2701 1001.545 15.9879 -6.9 -0.6000000000000005 9.75 4005.7 nuORFdb_human ENST00000640129.1_1_12:9723456-9724120:+ ENST00000640129.1_1_12:9723456-9724120:+ RP11-726G1.1 Out-of-Frame Out-of-Frame GN=RP11-726G1.1 Out-of-Frame E_20221201_NO30_400nL_HLAc1_2e7_directIP_titration_rep3_Slot2-4_1_3518 20mio-rep3 E_20221201_NO30_400nL_HLAc1_2e7_directIP_titration_rep4_Slot2-4_1_3519.9155.1463.2 2 3 1 16.26 6.27 8.24 9.99 87.7 8 0 1 110000.0 M17m Hydrogen 9 (H) 9 LSRPPLSTm (F) 9 SRPLPSLTm L/S/R|P|P\L|S|T\m 100.0 9 6 6 4 0 dual b/y bias m:Oxidized methionine 31.627209 0.836953 509.2696 1001.545 15.9879 -6.9 -1.1000000000000005 9.75 4005.7 nuORFdb_human ENST00000640129.1_1_12:9723456-9724120:+ ENST00000640129.1_1_12:9723456-9724120:+ RP11-726G1.1 Out-of-Frame Out-of-Frame GN=RP11-726G1.1 Out-of-Frame E_20221201_NO30_400nL_HLAc1_2e7_directIP_titration_rep4_Slot2-4_1_3519 40mio-rep1 E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep1_Slot1-04_1_3496.9451.1435.2 2 2 1 15.57 9.01 5.93 6.56 84.3 7 0 1 177000.0 M17m Hydrogen 9 (H) 9 LSRPPLSTm (F) 9 KKIFFSTm L/S/R|P P\L|S|T\m 77.8 7 5 5 3 0 dual b/y bias m:Oxidized methionine 32.650711 0.854411 509.2713 1001.545 15.9899 -4.9 -1.7000000000000002 9.75 4005.7 nuORFdb_human ENST00000640129.1_1_12:9723456-9724120:+ ENST00000640129.1_1_12:9723456-9724120:+ RP11-726G1.1 Out-of-Frame Out-of-Frame GN=RP11-726G1.1 Out-of-Frame E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep1_Slot1-04_1_3496 40mio-rep2 E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep2_Slot2-5_1_3520.9132.1454.2 2 2 1 17.87 8.44 7.39 9.43 93.9 8 0 1 196000.0 M17m Hydrogen 9 (H) 9 LSRPPLSTm (F) 9 TAAPLPLSmT L/S/R|P/P\L|S|T\m 100.0 9 5 6 3 0 dual b/y bias m:Oxidized methionine 31.548065 0.842534 509.271 1001.545 15.9899 -4.9 0.0 9.75 4005.7 nuORFdb_human ENST00000640129.1_1_12:9723456-9724120:+ ENST00000640129.1_1_12:9723456-9724120:+ RP11-726G1.1 Out-of-Frame Out-of-Frame GN=RP11-726G1.1 Out-of-Frame E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep2_Slot2-5_1_3520 40mio-rep3 E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep3_Slot2-5_1_3521.9132.1453.2 2 2 1 18.04 8.0 8.29 10.05 92.9 8 0 1 191000.0 M17m Hydrogen 9 (H) 9 LSRPPLSTm (F) 9 SRPLPSLTm L/S/R|P|P\L|S|T\m 100.0 9 6 6 4 0 dual b/y bias m:Oxidized methionine 31.549961 0.843277 509.2719 1001.545 15.9919 -3.0 1.5999999999999996 9.75 4005.7 nuORFdb_human ENST00000640129.1_1_12:9723456-9724120:+ ENST00000640129.1_1_12:9723456-9724120:+ RP11-726G1.1 Out-of-Frame Out-of-Frame GN=RP11-726G1.1 Out-of-Frame E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep3_Slot2-5_1_3521 40mio-rep4 E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep4_Slot2-5_1_3522.9154.1452.2 2 3 1 18.86 8.37 7.62 10.49 92.9 8 0 1 213000.0 M17m Hydrogen 9 (H) 9 LSRPPLSTm (F) 9 SRPLPSLTm L/S/R|P|P\L|S|T\m 100.0 9 6 6 4 0 dual b/y bias m:Oxidized methionine 31.616217 0.843818 509.2703 1001.545 15.9879 -6.9 -2.2 9.75 4005.7 nuORFdb_human ENST00000640129.1_1_12:9723456-9724120:+ ENST00000640129.1_1_12:9723456-9724120:+ RP11-726G1.1 Out-of-Frame Out-of-Frame GN=RP11-726G1.1 Out-of-Frame E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep4_Slot2-5_1_3522 5mio-rep1 E_20221201_NO30_400nL_HLAc1_5e6_directIP_titration_rep1_Slot1-37_1_3502.9430.1465.2 2 1 1 10.32 2.23 6.48 8.09 72.2 7 0 1 22600.0 M17m Hydrogen 9 (H) 9 LSRPPLSTm (F) 9 TAAPLPLSmT L/S/R|P/P L|S|T\m 77.8 7 4 6 3 0 dual b/y bias m:Oxidized methionine 32.577934 0.835712 509.2703 1001.545 15.9879 -6.9 0.09999999999999964 9.75 4005.7 nuORFdb_human ENST00000640129.1_1_12:9723456-9724120:+ ENST00000640129.1_1_12:9723456-9724120:+ RP11-726G1.1 Out-of-Frame Out-of-Frame GN=RP11-726G1.1 Out-of-Frame E_20221201_NO30_400nL_HLAc1_5e6_directIP_titration_rep1_Slot1-37_1_3502 5mio-rep2 E_20221201_NO30_400nL_HLAc1_5e6_directIP_titration_rep2_Slot2-2_1_3510.9386.1478.2 2 1 1 7.62 2.95 7.62 54.5 6 0 1 17900.0 M17m Hydrogen 9 (H) 9 LSRPPLSTm (F) 9 L/S R\P P/L|S/T\m 55.6 5 3 4 1 0 unbiased m:Oxidized methionine 32.420559 0.827229 509.2694 1001.545 15.9859 -8.9 -0.20000000000000107 9.75 4005.7 nuORFdb_human ENST00000640129.1_1_12:9723456-9724120:+ ENST00000640129.1_1_12:9723456-9724120:+ RP11-726G1.1 Out-of-Frame Out-of-Frame GN=RP11-726G1.1 Out-of-Frame E_20221201_NO30_400nL_HLAc1_5e6_directIP_titration_rep2_Slot2-2_1_3510 5mio-rep3 E_20221201_NO30_400nL_HLAc1_5e6_directIP_titration_rep3_Slot2-2_1_3511.9374.1475.2 2 3 1 9.41 2.08 5.31 7.33 63.7 8 0 1 25300.0 M17m Hydrogen 9 (H) 9 LSRPPLSTm (F) 9 SRPLPSLTm L/S/R|P/P\L|S|T\m 100.0 9 5 6 3 0 dual b/y bias m:Oxidized methionine 32.386829 0.829401 509.2689 1001.545 15.9859 -8.9 -0.7000000000000011 9.75 4005.7 nuORFdb_human ENST00000640129.1_1_12:9723456-9724120:+ ENST00000640129.1_1_12:9723456-9724120:+ RP11-726G1.1 Out-of-Frame Out-of-Frame GN=RP11-726G1.1 Out-of-Frame E_20221201_NO30_400nL_HLAc1_5e6_directIP_titration_rep3_Slot2-2_1_3511 10mio-rep1 E_20221201_NO30_400nL_HLAc1_1e7_directIP_titration_rep1_Slot2-3_1_3512.16822.1399.2 2 4 1 13.57 5.25 5.08 8.31 78.5 8 0 1 81600.0 M17m Hydrogen 9 (H) 10 LSRPPLSTmF (S) 10 LLPGLSVGnmF L/S/R|P\P L\S|T\m\F 80.0 8 6 4 2 0 unbiased m:Oxidized methionine 58.109802 0.876787 582.8021 1148.613 15.9835 -9.8 -2.1000000000000005 9.75 4005.7 nuORFdb_human ENST00000640129.1_1_12:9723456-9724120:+ ENST00000640129.1_1_12:9723456-9724120:+ RP11-726G1.1 Out-of-Frame Out-of-Frame GN=RP11-726G1.1 Out-of-Frame E_20221201_NO30_400nL_HLAc1_1e7_directIP_titration_rep1_Slot2-3_1_3512 10mio-rep2 E_20221201_NO30_400nL_HLAc1_1e7_directIP_titration_rep2_Slot2-3_1_3513.16843.1398.2 2 4 1 12.76 4.4 6.63 8.36 72.4 7 0 1 70300.0 M17m Hydrogen 9 (H) 10 LSRPPLSTmF (S) 10 VTPPILSSGmF L/S/R|P P L\S|T|m\F 70.0 7 5 5 3 0 unbiased m:Oxidized methionine 58.181889 0.877359 582.8042 1148.613 15.9875 -6.4 0.2999999999999998 9.75 4005.7 nuORFdb_human ENST00000640129.1_1_12:9723456-9724120:+ ENST00000640129.1_1_12:9723456-9724120:+ RP11-726G1.1 Out-of-Frame Out-of-Frame GN=RP11-726G1.1 Out-of-Frame E_20221201_NO30_400nL_HLAc1_1e7_directIP_titration_rep2_Slot2-3_1_3513 10mio-rep3 E_20221201_NO30_400nL_HLAc1_1e7_directIP_titration_rep3_Slot2-3_1_3514.16733.1400.2 2 5 1 15.04 5.93 6.58 9.11 78.0 7 0 1 103000.0 M17m Hydrogen 9 (H) 10 LSRPPLSTmF (S) 10 PIKDnALVmF L/S/R|P P L\S|T\m\F 70.0 7 5 4 2 0 unbiased m:Oxidized methionine 57.802498 0.87659 582.8045 1148.613 15.9895 -4.7 1.5999999999999996 9.75 4005.7 nuORFdb_human ENST00000640129.1_1_12:9723456-9724120:+ ENST00000640129.1_1_12:9723456-9724120:+ RP11-726G1.1 Out-of-Frame Out-of-Frame GN=RP11-726G1.1 Out-of-Frame E_20221201_NO30_400nL_HLAc1_1e7_directIP_titration_rep3_Slot2-3_1_3514 20mio-rep1 E_20221201_NO30_400nL_HLAc1_2e7_directIP_titration_rep1_Slot2-4_1_3515.16821.1396.2 2 5 1 15.7 6.5 6.07 9.21 87.0 7 0 1 177000.0 M17m Hydrogen 9 (H) 10 LSRPPLSTmF (S) 10 VTPPILSSGmF L/S/R|P P L\S|T|m\F 70.0 7 5 5 3 0 unbiased m:Oxidized methionine 58.092361 0.879123 582.8045 1148.613 15.9875 -6.4 -0.20000000000000018 9.75 4005.7 nuORFdb_human ENST00000640129.1_1_12:9723456-9724120:+ ENST00000640129.1_1_12:9723456-9724120:+ RP11-726G1.1 Out-of-Frame Out-of-Frame GN=RP11-726G1.1 Out-of-Frame E_20221201_NO30_400nL_HLAc1_2e7_directIP_titration_rep1_Slot2-4_1_3515 20mio-rep2 E_20221201_NO30_400nL_HLAc1_2e7_directIP_titration_rep3_Slot2-4_1_3518.16793.1393.2 2 5 1 15.73 6.71 6.8 9.02 84.4 7 0 1 156000.0 M17m Hydrogen 9 (H) 10 LSRPPLSTmF (S) 10 TILREPAAmF L S/R|P P/L\S|T|m\F 60.0 6 5 5 3 0 unbiased m:Oxidized methionine 58.004196 0.881034 582.8046 1148.613 15.9895 -4.7 1.5999999999999996 9.75 4005.7 nuORFdb_human ENST00000640129.1_1_12:9723456-9724120:+ ENST00000640129.1_1_12:9723456-9724120:+ RP11-726G1.1 Out-of-Frame Out-of-Frame GN=RP11-726G1.1 Out-of-Frame E_20221201_NO30_400nL_HLAc1_2e7_directIP_titration_rep3_Slot2-4_1_3518 20mio-rep3 E_20221201_NO30_400nL_HLAc1_2e7_directIP_titration_rep4_Slot2-4_1_3519.16865.1386.2 2 5 1 16.92 7.54 6.89 9.38 89.3 8 0 1 192000.0 M17m Hydrogen 9 (H) 10 LSRPPLSTmF (S) 10 VTPPILSSGmF L/S/R|P P\L\S|T|m\F 80.0 8 6 5 3 0 unbiased m:Oxidized methionine 58.251644 0.885287 582.8046 1148.613 15.9895 -4.7 1.0999999999999996 9.75 4005.7 nuORFdb_human ENST00000640129.1_1_12:9723456-9724120:+ ENST00000640129.1_1_12:9723456-9724120:+ RP11-726G1.1 Out-of-Frame Out-of-Frame GN=RP11-726G1.1 Out-of-Frame E_20221201_NO30_400nL_HLAc1_2e7_directIP_titration_rep4_Slot2-4_1_3519 40mio-rep1 E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep1_Slot1-04_1_3496.17009.1353.2 2 6 1 17.35 6.71 7.94 10.64 88.3 8 0 1 171000.0 M17m Hydrogen 9 (H) 10 LSRPPLSTmF (S) 10 TILREPAAmF L/S/R|P/P L\S|T|m\F 80.0 8 5 6 3 0 unbiased m:Oxidized methionine 58.753513 0.905822 582.8071 1148.613 15.9935 -1.2 2.0 9.75 4005.7 nuORFdb_human ENST00000640129.1_1_12:9723456-9724120:+ ENST00000640129.1_1_12:9723456-9724120:+ RP11-726G1.1 Out-of-Frame Out-of-Frame GN=RP11-726G1.1 Out-of-Frame E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep1_Slot1-04_1_3496 40mio-rep2 E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep2_Slot2-5_1_3520.16854.1375.2 2 6 1 17.74 6.76 7.8 10.98 89.0 8 0 1 406000.0 M17m Hydrogen 9 (H) 10 LSRPPLSTmF (S) 10 TILREPAAmF L/S/R|P/P L\S|T|m\F 80.0 8 5 6 3 0 unbiased m:Oxidized methionine 58.219742 0.891755 582.8061 1148.613 15.9915 -2.9 2.0000000000000004 9.75 4005.7 nuORFdb_human ENST00000640129.1_1_12:9723456-9724120:+ ENST00000640129.1_1_12:9723456-9724120:+ RP11-726G1.1 Out-of-Frame Out-of-Frame GN=RP11-726G1.1 Out-of-Frame E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep2_Slot2-5_1_3520 40mio-rep3 E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep3_Slot2-5_1_3521.16854.1381.2 2 5 1 17.99 7.78 8.08 10.21 91.5 8 0 1 317000.0 M17m Hydrogen 9 (H) 10 LSRPPLSTmF (S) 10 VTPPILSSGmF L/S/R|P\P L\S|T|m\F 80.0 8 6 5 3 0 unbiased m:Oxidized methionine 58.220589 0.888471 582.8055 1148.613 15.9915 -2.9 1.6999999999999997 9.75 4005.7 nuORFdb_human ENST00000640129.1_1_12:9723456-9724120:+ ENST00000640129.1_1_12:9723456-9724120:+ RP11-726G1.1 Out-of-Frame Out-of-Frame GN=RP11-726G1.1 Out-of-Frame E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep3_Slot2-5_1_3521 40mio-rep4 E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep4_Slot2-5_1_3522.16854.1377.2 2 6 1 17.69 9.0 6.18 8.69 90.5 7 0 1 369000.0 M17m Hydrogen 9 (H) 10 LSRPPLSTmF (S) 10 VTPPILSSGmF L/S/R|P P L\S|T|m\F 70.0 7 5 5 3 0 unbiased m:Oxidized methionine 58.204952 0.890985 582.8057 1148.613 15.9915 -2.9 1.8000000000000003 9.75 4005.7 nuORFdb_human ENST00000640129.1_1_12:9723456-9724120:+ ENST00000640129.1_1_12:9723456-9724120:+ RP11-726G1.1 Out-of-Frame Out-of-Frame GN=RP11-726G1.1 Out-of-Frame E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep4_Slot2-5_1_3522 5mio-rep1 E_20221201_NO30_400nL_HLAc1_5e6_directIP_titration_rep1_Slot1-37_1_3502.17063.1387.2 2 2 1 15.81 6.34 6.1 9.47 83.3 8 0 1 28000.0 M17m Hydrogen 9 (H) 10 LSRPPLSTmF (S) 10 LLPGLSVGnmF L/S/R|P\P L\S|T|m\F 80.0 8 6 5 3 0 unbiased m:Oxidized methionine 58.938881 0.884295 582.8046 1148.613 15.9895 -4.7 2.3 9.75 4005.7 nuORFdb_human ENST00000640129.1_1_12:9723456-9724120:+ ENST00000640129.1_1_12:9723456-9724120:+ RP11-726G1.1 Out-of-Frame Out-of-Frame GN=RP11-726G1.1 Out-of-Frame E_20221201_NO30_400nL_HLAc1_5e6_directIP_titration_rep1_Slot1-37_1_3502 5mio-rep2 E_20221201_NO30_400nL_HLAc1_5e6_directIP_titration_rep2_Slot2-2_1_3510.16976.1403.2 2 4 1 12.97 6.85 5.24 6.13 71.7 7 0 1 20500.0 M17m Hydrogen 9 (H) 10 LSRPPLSTmF (S) 10 PIKDnALVmF L/S R|P/P L\S|T|m\F 60.0 6 5 5 3 0 unbiased m:Oxidized methionine 58.63636 0.874496 582.801 1148.613 15.9815 -11.5 -2.8000000000000007 9.75 4005.7 nuORFdb_human ENST00000640129.1_1_12:9723456-9724120:+ ENST00000640129.1_1_12:9723456-9724120:+ RP11-726G1.1 Out-of-Frame Out-of-Frame GN=RP11-726G1.1 Out-of-Frame E_20221201_NO30_400nL_HLAc1_5e6_directIP_titration_rep2_Slot2-2_1_3510 5mio-rep3 E_20221201_NO30_400nL_HLAc1_5e6_directIP_titration_rep3_Slot2-2_1_3511.16931.1399.2 2 2 1 13.53 6.05 6.26 7.48 77.3 6 0 1 18100.0 M17m Hydrogen 9 (H) 10 LSRPPLSTmF (S) 10 VTPPILSSGmF L S R|P\P L\S|T|m\F 50.0 5 6 3 3 0 b-ion bias m:Oxidized methionine 58.482677 0.877072 582.8036 1148.613 15.9875 -6.4 1.799999999999999 9.75 4005.7 nuORFdb_human ENST00000640129.1_1_12:9723456-9724120:+ ENST00000640129.1_1_12:9723456-9724120:+ RP11-726G1.1 Out-of-Frame Out-of-Frame GN=RP11-726G1.1 Out-of-Frame E_20221201_NO30_400nL_HLAc1_5e6_directIP_titration_rep3_Slot2-2_1_3511 40mio-rep3 E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep3_Slot2-5_1_3521.24391.525.1 1 1 1 11.43 8.67 5.2 2.76 86.5 6 0 0 5900.0 Hydrogen 34 (T) 9 LSVGSVTVW (M) 9 ISTQTLVEG L/S V\G S|V\T\V\W 55.6 5 5 2 1 0 b-ion bias 84.256516 1.411358 947.5164 947.52 -0.0037 -3.9 0.6999999999999997 5.52 6127.9 nuORFdb_human ENST00000494257.1_1_1:3407490-3409358:- ENST00000494257.1_1_1:3407490-3409358:-|ENST00000356575.8_1_1:3407490-3411251:-|ENST00000294599.8_1_1:3407490-3411287:- MEGF6 3' Overlap dORF 3' Overlap dORF GN=MEGF6 3' Overlap dORF E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep3_Slot2-5_1_3521 20mio-rep1 E_20221201_NO30_400nL_HLAc1_2e7_directIP_titration_rep1_Slot2-4_1_3515.26663.537.1 1 1 1 11.63 7.39 7.35 4.24 79.1 6 0 0 2660.0 Hydrogen 20 (R) 8 LTAAPWTW (G) 8 LTAATWPW L T/A/A|P|W\T\W 75.0 6 4 4 2 0 unbiased 92.071983 1.403732 945.4735 945.483 -0.0099 -10.4 -4.2 5.52 5606.3 nuORFdb_human ENST00000495579.5_1_6:32094959-32095503:- ENST00000495579.5_1_6:32094959-32095503:- ATF6B ncRNA Retained Intron lncRNA GN=ATF6B ncRNA Retained Intron E_20221201_NO30_400nL_HLAc1_2e7_directIP_titration_rep1_Slot2-4_1_3515 20mio-rep2 E_20221201_NO30_400nL_HLAc1_2e7_directIP_titration_rep3_Slot2-4_1_3518.26695.526.1 1 1 1 12.56 6.63 8.01 5.93 82.5 5 0 0 2690.0 Hydrogen 20 (R) 8 LTAAPWTW (G) 8 LTAATWPW L T A/A|P|W|T\W 62.5 5 4 4 3 0 dual b/y bias 92.201385 1.410401 945.4779 945.483 -0.0049 -5.2 1.0999999999999996 5.52 5606.3 nuORFdb_human ENST00000495579.5_1_6:32094959-32095503:- ENST00000495579.5_1_6:32094959-32095503:- ATF6B ncRNA Retained Intron lncRNA GN=ATF6B ncRNA Retained Intron E_20221201_NO30_400nL_HLAc1_2e7_directIP_titration_rep3_Slot2-4_1_3518 20mio-rep3 E_20221201_NO30_400nL_HLAc1_2e7_directIP_titration_rep4_Slot2-4_1_3519.26740.528.1 1 2 1 12.15 7.77 7.63 4.38 85.5 6 0 0 4840.0 Hydrogen 20 (R) 8 LTAAPWTW (G) 8 LTAATWPW L T/A/A/P|W|T\W 75.0 6 3 5 2 0 unbiased 92.353203 1.409191 945.4802 945.483 -0.0029 -3.0 2.8 5.52 5606.3 nuORFdb_human ENST00000495579.5_1_6:32094959-32095503:- ENST00000495579.5_1_6:32094959-32095503:- ATF6B ncRNA Retained Intron lncRNA GN=ATF6B ncRNA Retained Intron E_20221201_NO30_400nL_HLAc1_2e7_directIP_titration_rep4_Slot2-4_1_3519 40mio-rep1 E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep1_Slot1-04_1_3496.26932.508.1 1 1 1 11.55 7.12 6.76 4.43 72.1 6 0 0 7810.0 Hydrogen 20 (R) 8 LTAAPWTW (G) 8 LTAATWPW L T/A/A|P\W|T\W 75.0 6 4 4 2 0 unbiased 93.039993 1.421179 945.4791 945.483 -0.0039 -4.1 -0.8999999999999995 5.52 5606.3 nuORFdb_human ENST00000495579.5_1_6:32094959-32095503:- ENST00000495579.5_1_6:32094959-32095503:- ATF6B ncRNA Retained Intron lncRNA GN=ATF6B ncRNA Retained Intron E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep1_Slot1-04_1_3496 40mio-rep2 E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep2_Slot2-5_1_3520.26701.527.1 1 1 1 9.31 7.52 4.98 1.79 65.2 5 0 0 6680.0 Hydrogen 20 (R) 8 LTAAPWTW (G) 8 LTAATWPW L T/A|A|P W\T\W 50.0 4 4 3 2 0 unbiased 92.239937 1.410024 945.4764 945.483 -0.0069 -7.3 -2.3999999999999995 5.52 5606.3 nuORFdb_human ENST00000495579.5_1_6:32094959-32095503:- ENST00000495579.5_1_6:32094959-32095503:- ATF6B ncRNA Retained Intron lncRNA GN=ATF6B ncRNA Retained Intron E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep2_Slot2-5_1_3520 40mio-rep3 E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep3_Slot2-5_1_3521.26712.524.1 1 1 1 10.08 6.83 6.43 3.25 69.2 6 0 0 6110.0 Hydrogen 20 (R) 8 LTAAPWTW (G) 8 LTAATWPW L T/A/A|P\W/T\W 75.0 6 3 4 1 0 unbiased 92.2715 1.411634 945.48 945.483 -0.0029 -3.0 1.5999999999999996 5.52 5606.3 nuORFdb_human ENST00000495579.5_1_6:32094959-32095503:- ENST00000495579.5_1_6:32094959-32095503:- ATF6B ncRNA Retained Intron lncRNA GN=ATF6B ncRNA Retained Intron E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep3_Slot2-5_1_3521 40mio-rep4 E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep4_Slot2-5_1_3522.26713.522.1 1 1 1 9.07 5.33 3.67 3.74 69.6 5 0 0 5360.0 Hydrogen 20 (R) 8 LTAAPWTW (G) 8 LTAATWPW L T A/A|P|W\T\W 62.5 5 4 3 2 0 unbiased 92.267586 1.412961 945.4797 945.483 -0.0029 -3.0 1.7000000000000002 5.52 5606.3 nuORFdb_human ENST00000495579.5_1_6:32094959-32095503:- ENST00000495579.5_1_6:32094959-32095503:- ATF6B ncRNA Retained Intron lncRNA GN=ATF6B ncRNA Retained Intron E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep4_Slot2-5_1_3522 40mio-rep4 E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep4_Slot2-5_1_3522.13792.646.1 1 1 1 9.58 4.4 3.98 5.17 68.1 7 0 0 3320.0 Hydrogen 16 (F) 9 LTAGASASW (R) 9 IGKnSASW L T/A/G/A|S\A\S\W 77.8 7 4 4 1 0 unbiased 47.630821 1.338541 863.4245 863.426 -0.0018 -2.0 2.7 5.52 3993.5 nuORFdb_human ENST00000555589.5_1_14:93022139-93022250:+ ENST00000555589.5_1_14:93022139-93022250:+ RIN3 Out-of-Frame Out-of-Frame GN=RIN3 Out-of-Frame E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep4_Slot2-5_1_3522 40mio-rep1 E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep1_Slot1-04_1_3496.13396.704.1 1 1 1 7.98 3.1 4.78 4.88 59.2 6 0 0 2730.0 Hydrogen 1513 (R) 8 LTASSASW (I) 8 IASSTASW L T/A/S\S|A|S\W 75.0 6 4 4 2 0 unbiased 46.274097 1.304031 822.3989 822.399 -0.0002 -0.3 2.9000000000000004 5.52 173307.9 nuORFdb_human ENST00000588552.5_1_19:5159112-5286160:- ENST00000588552.5_1_19:5159112-5286160:-|ENST00000486927.1_1_2:38585669-38586665:- PTPRS 5' Overlap uORF 5' Overlap uORF GN=PTPRS 5' Overlap uORF E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep1_Slot1-04_1_3496 10mio-rep1 E_20221201_NO30_400nL_HLAc1_1e7_directIP_titration_rep1_Slot2-3_1_3512.24850.269.1 1 1 1 10.82 5.47 3.65 5.35 75.4 5 0 0 2500.0 Hydrogen 24 (I) 9 LTDEAFEFY (C) 9 IVnnFSEFY L T D E\A/F\E|F\Y 55.6 5 4 2 1 0 b-ion bias 85.835587 1.562538 1134.4869 1134.499 -0.009 -7.9 -0.20000000000000018 3.57 7867.8 nuORFdb_human ENST00000495663.1_1_16:538965-546860:+ ENST00000495663.1_1_16:538965-546860:+ RAB11FIP3 ncRNA Retained Intron lncRNA GN=RAB11FIP3 ncRNA Retained Intron E_20221201_NO30_400nL_HLAc1_1e7_directIP_titration_rep1_Slot2-3_1_3512 10mio-rep3 E_20221201_NO30_400nL_HLAc1_1e7_directIP_titration_rep3_Slot2-3_1_3514.24750.267.1 1 2 1 11.68 6.03 7.22 5.65 77.7 5 0 0 4090.0 Hydrogen 24 (I) 9 LTDEAFEFY (C) 9 LnmLSTEFY L T D E\A|F\E|F\Y 55.6 5 5 2 2 0 b-ion bias 85.490067 1.563676 1134.489 1134.499 -0.009 -7.9 -1.6000000000000005 3.57 7867.8 nuORFdb_human ENST00000495663.1_1_16:538965-546860:+ ENST00000495663.1_1_16:538965-546860:+ RAB11FIP3 ncRNA Retained Intron lncRNA GN=RAB11FIP3 ncRNA Retained Intron E_20221201_NO30_400nL_HLAc1_1e7_directIP_titration_rep3_Slot2-3_1_3514 20mio-rep1 E_20221201_NO30_400nL_HLAc1_2e7_directIP_titration_rep1_Slot2-4_1_3515.24733.263.1 1 1 1 12.23 4.03 9.6 8.19 81.4 5 0 0 4510.0 Hydrogen 24 (I) 9 LTDEAFEFY (C) 9 LcNKVEFY L T D E\A|F\E|F\Y 55.6 5 5 2 2 0 b-ion bias 85.408447 1.566173 1134.4931 1134.499 -0.009 -7.9 -1.7000000000000002 3.57 7867.8 nuORFdb_human ENST00000495663.1_1_16:538965-546860:+ ENST00000495663.1_1_16:538965-546860:+ RAB11FIP3 ncRNA Retained Intron lncRNA GN=RAB11FIP3 ncRNA Retained Intron E_20221201_NO30_400nL_HLAc1_2e7_directIP_titration_rep1_Slot2-4_1_3515 20mio-rep2 E_20221201_NO30_400nL_HLAc1_2e7_directIP_titration_rep3_Slot2-4_1_3518.24751.256.1 1 1 1 12.77 6.04 8.04 6.73 86.6 5 0 0 3190.0 Hydrogen 24 (I) 9 LTDEAFEFY (C) 9 IVnnFSEFY L T D E-A|F\E|F\Y 55.6 5 4 2 2 1 b-ion bias 85.489975 1.57014 1134.4948 1134.499 -0.009 -7.9 -1.6000000000000005 3.57 7867.8 nuORFdb_human ENST00000495663.1_1_16:538965-546860:+ ENST00000495663.1_1_16:538965-546860:+ RAB11FIP3 ncRNA Retained Intron lncRNA GN=RAB11FIP3 ncRNA Retained Intron E_20221201_NO30_400nL_HLAc1_2e7_directIP_titration_rep3_Slot2-4_1_3518 20mio-rep3 E_20221201_NO30_400nL_HLAc1_2e7_directIP_titration_rep4_Slot2-4_1_3519.24782.253.1 1 2 1 13.04 5.13 6.82 7.91 84.5 5 0 0 5930.0 Hydrogen 24 (I) 9 LTDEAFEFY (C) 9 LcNKVEFY L T D E\A|F\E|F\Y 55.6 5 5 2 2 0 b-ion bias 85.587746 1.571937 1134.4915 1134.499 -0.009 -7.9 -2.1000000000000005 3.57 7867.8 nuORFdb_human ENST00000495663.1_1_16:538965-546860:+ ENST00000495663.1_1_16:538965-546860:+ RAB11FIP3 ncRNA Retained Intron lncRNA GN=RAB11FIP3 ncRNA Retained Intron E_20221201_NO30_400nL_HLAc1_2e7_directIP_titration_rep4_Slot2-4_1_3519 40mio-rep1 E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep1_Slot1-04_1_3496.24954.236.1 1 3 1 15.84 5.83 10.25 10.01 95.3 5 0 0 30600.0 Hydrogen 24 (I) 9 LTDEAFEFY (C) 9 IVnnFSEFY L T D E\A|F\E|F\Y 55.6 5 5 2 2 0 b-ion bias 86.206535 1.581999 1134.495 1134.499 -0.009 -7.9 -4.7 3.57 7867.8 nuORFdb_human ENST00000495663.1_1_16:538965-546860:+ ENST00000495663.1_1_16:538965-546860:+ RAB11FIP3 ncRNA Retained Intron lncRNA GN=RAB11FIP3 ncRNA Retained Intron E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep1_Slot1-04_1_3496 40mio-rep3 E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep3_Slot2-5_1_3521.24711.255.1 1 2 1 13.94 4.99 8.86 8.95 90.8 5 0 0 9920.0 Hydrogen 24 (I) 9 LTDEAFEFY (C) 9 IVnnFSEFY L T D E\A|F\E|F\Y 55.6 5 5 2 2 0 b-ion bias 85.362305 1.570767 1134.4933 1134.499 -0.009 -7.9 -3.3000000000000007 3.57 7867.8 nuORFdb_human ENST00000495663.1_1_16:538965-546860:+ ENST00000495663.1_1_16:538965-546860:+ RAB11FIP3 ncRNA Retained Intron lncRNA GN=RAB11FIP3 ncRNA Retained Intron E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep3_Slot2-5_1_3521 40mio-rep4 E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep4_Slot2-5_1_3522.24722.256.1 1 2 1 14.71 6.96 10.01 7.74 86.7 5 0 0 7440.0 Hydrogen 24 (I) 9 LTDEAFEFY (C) 9 LnmLSTEFY L T D E|A|F\E|F\Y 55.6 5 5 3 3 0 dual b/y bias 85.388313 1.570492 1134.4909 1134.499 -0.009 -7.9 -3.2 3.57 7867.8 nuORFdb_human ENST00000495663.1_1_16:538965-546860:+ ENST00000495663.1_1_16:538965-546860:+ RAB11FIP3 ncRNA Retained Intron lncRNA GN=RAB11FIP3 ncRNA Retained Intron E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep4_Slot2-5_1_3522 5mio-rep1 E_20221201_NO30_400nL_HLAc1_5e6_directIP_titration_rep1_Slot1-37_1_3502.25048.256.1 1 1 1 10.4 1.67 7.58 8.72 79.7 5 0 0 1430.0 Hydrogen 24 (I) 9 LTDEAFEFY (C) 9 LcNKVEFY L T D E\A|F\E\F\Y 55.6 5 5 1 1 0 b-ion bias 86.510887 1.570078 1134.4893 1134.499 -0.009 -7.9 -0.9000000000000004 3.57 7867.8 nuORFdb_human ENST00000495663.1_1_16:538965-546860:+ ENST00000495663.1_1_16:538965-546860:+ RAB11FIP3 ncRNA Retained Intron lncRNA GN=RAB11FIP3 ncRNA Retained Intron E_20221201_NO30_400nL_HLAc1_5e6_directIP_titration_rep1_Slot1-37_1_3502 40mio-rep1 E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep1_Slot1-04_1_3496.16886.1368.2 2 2 1 14.54 5.95 6.65 8.59 76.7 8 1 1 18700.0 Hydrogen 1 (-) 9 LTDQSRWSW (R) 9 LTQRICDCL L/T|D/Q/S|R|W|S\W 100.0 9 5 7 4 0 dual b/y bias 58.328815 0.896294 589.7831 1178.559 -0.0002 -0.1 3.1 5.84 5607.1 nuORFdb_human ENST00000585242.5_1_17:61904857-61907287:+ ENST00000585242.5_1_17:61904857-61907287:+|ENST00000579147.5_1_17:61904821-61905570:+|ENST00000310144.10_1_17:61904677-61906854:+ PSMC5 Out-of-Frame Out-of-Frame GN=PSMC5 Out-of-Frame E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep1_Slot1-04_1_3496 40mio-rep2 E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep2_Slot2-5_1_3520.16542.1395.2 2 1 1 13.25 5.93 6.99 7.31 78.8 8 1 1 7710.0 Hydrogen 1 (-) 9 LTDQSRWSW (R) 9 LATGDDIYDVP L/T/D/Q|S\R|W|S\W 100.0 9 5 6 3 0 dual b/y bias 57.142071 0.879725 589.7788 1178.559 -0.0082 -6.9 -2.0 5.84 5607.1 nuORFdb_human ENST00000585242.5_1_17:61904857-61907287:+ ENST00000585242.5_1_17:61904857-61907287:+|ENST00000579147.5_1_17:61904821-61905570:+|ENST00000310144.10_1_17:61904677-61906854:+ PSMC5 Out-of-Frame Out-of-Frame GN=PSMC5 Out-of-Frame E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep2_Slot2-5_1_3520 40mio-rep3 E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep3_Slot2-5_1_3521.16565.1390.2 2 1 1 13.54 3.03 7.67 10.51 72.9 8 1 1 7860.0 Hydrogen 1 (-) 9 LTDQSRWSW (R) 9 LTLANAGCNSAS L/T/D/Q|S/R|W|S\W 100.0 9 4 7 3 0 dual b/y bias 57.222099 0.882683 589.7793 1178.559 -0.0082 -6.9 -2.3000000000000007 5.84 5607.1 nuORFdb_human ENST00000585242.5_1_17:61904857-61907287:+ ENST00000585242.5_1_17:61904857-61907287:+|ENST00000579147.5_1_17:61904821-61905570:+|ENST00000310144.10_1_17:61904677-61906854:+ PSMC5 Out-of-Frame Out-of-Frame GN=PSMC5 Out-of-Frame E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep3_Slot2-5_1_3521 40mio-rep4 E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep4_Slot2-5_1_3522.16542.1391.2 2 1 1 9.29 9.29 9.29 58.4 8 1 1 9700.0 Hydrogen 1 (-) 9 LTDQSRWSW (R) 9 L/T/D\Q|S|R|W\S\W 100.0 9 6 5 3 0 dual b/y bias 57.12801 0.88224 589.7797 1178.559 -0.0062 -5.2 -0.5 5.84 5607.1 nuORFdb_human ENST00000585242.5_1_17:61904857-61907287:+ ENST00000585242.5_1_17:61904857-61907287:+|ENST00000579147.5_1_17:61904821-61905570:+|ENST00000310144.10_1_17:61904677-61906854:+ PSMC5 Out-of-Frame Out-of-Frame GN=PSMC5 Out-of-Frame E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep4_Slot2-5_1_3522 10mio-rep1 E_20221201_NO30_400nL_HLAc1_1e7_directIP_titration_rep1_Slot2-3_1_3512.15951.644.1 1 1 1 7.91 6.17 1.14 1.75 78.2 6 0 0 2050.0 Hydrogen 4 (R) 9 LTGDPPALA (F) 9 EEKPAPLA L T G/D/P\P\A\L\A 66.7 6 4 2 0 0 b-ion bias 55.10339 1.339698 854.458 854.462 -0.0038 -4.5 3.2 3.8 4557.0 nuORFdb_human ENST00000393207.5_1_16:88003716-88008722:+ ENST00000393207.5_1_16:88003716-88008722:+ BANP 5' Overlap uORF 5' Overlap uORF GN=BANP 5' Overlap uORF E_20221201_NO30_400nL_HLAc1_1e7_directIP_titration_rep1_Slot2-3_1_3512 20mio-rep3 E_20221201_NO30_400nL_HLAc1_2e7_directIP_titration_rep4_Slot2-4_1_3519.15838.631.1 1 1 1 10.79 9.49 1.17 1.3 87.5 5 0 0 2970.0 Hydrogen 4 (R) 9 LTGDPPALA (F) 9 EEKPAPLA L T G D|P|P\A\L\A 55.6 5 5 2 2 0 b-ion bias 54.705284 1.347739 854.4576 854.462 -0.0038 -4.5 1.2999999999999998 3.8 4557.0 nuORFdb_human ENST00000393207.5_1_16:88003716-88008722:+ ENST00000393207.5_1_16:88003716-88008722:+ BANP 5' Overlap uORF 5' Overlap uORF GN=BANP 5' Overlap uORF E_20221201_NO30_400nL_HLAc1_2e7_directIP_titration_rep4_Slot2-4_1_3519 40mio-rep1 E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep1_Slot1-04_1_3496.16103.617.1 1 1 1 11.65 8.84 2.21 2.81 87.9 6 0 0 4280.0 Hydrogen 4 (R) 9 LTGDPPALA (F) 9 EEKPAPLA L T G/D|P|P\A\L\A 66.7 6 5 3 2 0 unbiased 55.624409 1.355826 854.4567 854.462 -0.0048 -5.6 -2.3999999999999995 3.8 4557.0 nuORFdb_human ENST00000393207.5_1_16:88003716-88008722:+ ENST00000393207.5_1_16:88003716-88008722:+ BANP 5' Overlap uORF 5' Overlap uORF GN=BANP 5' Overlap uORF E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep1_Slot1-04_1_3496 40mio-rep2 E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep2_Slot2-5_1_3520.15779.635.1 1 1 1 10.8 9.11 1.57 1.68 88.1 5 0 0 7460.0 Hydrogen 4 (R) 9 LTGDPPALA (F) 9 EEKPAPLA L T G D|P|P\A\L\A 55.6 5 5 2 2 0 b-ion bias 54.506798 1.345406 854.4567 854.462 -0.0048 -5.6 -0.6999999999999993 3.8 4557.0 nuORFdb_human ENST00000393207.5_1_16:88003716-88008722:+ ENST00000393207.5_1_16:88003716-88008722:+ BANP 5' Overlap uORF 5' Overlap uORF GN=BANP 5' Overlap uORF E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep2_Slot2-5_1_3520 40mio-rep3 E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep3_Slot2-5_1_3521.15778.633.1 1 1 1 8.41 6.73 1.64 1.68 78.9 6 0 0 6080.0 Hydrogen 4 (R) 9 LTGDPPALA (F) 9 EEKPAPLA L T G/D/P\P\A\L\A 66.7 6 4 2 0 0 b-ion bias 54.504601 1.346809 854.458 854.462 -0.0038 -4.5 0.09999999999999964 3.8 4557.0 nuORFdb_human ENST00000393207.5_1_16:88003716-88008722:+ ENST00000393207.5_1_16:88003716-88008722:+ BANP 5' Overlap uORF 5' Overlap uORF GN=BANP 5' Overlap uORF E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep3_Slot2-5_1_3521 40mio-rep4 E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep4_Slot2-5_1_3522.15768.630.1 1 1 1 11.2 8.97 2.16 2.23 87.6 6 0 0 5060.0 Hydrogen 4 (R) 9 LTGDPPALA (F) 9 EEKPAPLA L T G/D|P|P\A\L\A 66.7 6 5 3 2 0 unbiased 54.455307 1.348153 854.4573 854.462 -0.0048 -5.6 -0.8999999999999995 3.8 4557.0 nuORFdb_human ENST00000393207.5_1_16:88003716-88008722:+ ENST00000393207.5_1_16:88003716-88008722:+ BANP 5' Overlap uORF 5' Overlap uORF GN=BANP 5' Overlap uORF E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep4_Slot2-5_1_3522 40mio-rep1 E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep1_Slot1-04_1_3496.13651.540.1 1 1 1 9.59 8.06 2.91 1.53 77.1 6 0 0 6070.0 Hydrogen 6 (H) 9 LTPQADTGL (L) 9 TIQPEGGTI L/T/P Q\A D\T-G\L 55.6 5 3 2 0 1 unbiased 47.1549 1.40243 915.4755 915.478 -0.0022 -2.4 0.8000000000000003 3.8 5125.0 nuORFdb_human ENST00000541110.5_2_6:42330765-42418997:- ENST00000541110.5_2_6:42330765-42418997:- TRERF1 5' uORF 5' uORF GN=TRERF1 5' uORF E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep1_Slot1-04_1_3496 40mio-rep3 E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep3_Slot2-5_1_3521.13332.551.1 1 1 1 9.98 8.41 3.29 1.57 76.5 6 0 0 3600.0 Hydrogen 6 (H) 9 LTPQADTGL (L) 9 TIQPEGGTI L/T/P Q|A D\T-G\L 55.6 5 3 3 1 1 unbiased 46.059311 1.395278 915.4746 915.478 -0.0032 -3.5 1.0999999999999996 3.8 5125.0 nuORFdb_human ENST00000541110.5_2_6:42330765-42418997:- ENST00000541110.5_2_6:42330765-42418997:- TRERF1 5' uORF 5' uORF GN=TRERF1 5' uORF E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep3_Slot2-5_1_3521 40mio-rep4 E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep4_Slot2-5_1_3522.13331.552.1 1 1 1 7.38 5.65 2.24 1.73 71.7 5 0 0 2580.0 Hydrogen 6 (H) 9 LTPQADTGL (L) 9 LTADPGASAI L T/P Q\A D\T-G\L 33.3 3 3 1 0 1 b-ion bias 46.036831 1.39471 915.4719 915.478 -0.0062 -6.8 -2.0999999999999996 3.8 5125.0 nuORFdb_human ENST00000541110.5_2_6:42330765-42418997:- ENST00000541110.5_2_6:42330765-42418997:- TRERF1 5' uORF 5' uORF GN=TRERF1 5' uORF E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep4_Slot2-5_1_3522 40mio-rep1 E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep1_Slot1-04_1_3496.10620.1472.2 2 1 1 13.28 11.73 3.22 1.56 87.3 6 1 1 9260.0 Hydrogen 11 (T) 8 LTRQATVW (R) 8 TIQRATVW L/T/R Q\A|T|V\W 75.0 6 4 4 2 0 unbiased 36.687706 0.831071 487.7722 974.542 -0.0051 -5.2 -2.0 9.75 3132.5 nuORFdb_human ENST00000356493.9_1_9:35101762-35101938:- ENST00000356493.9_1_9:35101762-35101938:- STOML2 Out-of-Frame Out-of-Frame GN=STOML2 Out-of-Frame E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep1_Slot1-04_1_3496 40mio-rep1 E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep1_Slot1-04_1_3496.23770.1399.2 2 1 1 11.3 9.23 0.78 2.07 82.8 5 0 0 8300.0 Hydrogen 1 (-) 9 LTSHLLISL (S) 9 LLSTLHLLS L T S H\L\L|I|S\L 55.6 5 5 2 2 0 b-ion bias 82.115028 0.877144 498.807 996.609 -0.0021 -2.1 1.1 6.74 3303.9 nuORFdb_human ENST00000530958.5_1_11:108094184-108094271:+ ENST00000530958.5_1_11:108094184-108094271:+ ATM 5' uORF 5' uORF GN=ATM 5' uORF E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep1_Slot1-04_1_3496 40mio-rep1 E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep1_Slot1-04_1_3496.18041.438.1 1 2 1 8.81 -0.94 8.48 9.75 64.3 7 0 0 8040.0 M17m Hydrogen 12 (A) 9 LTVTAmSSW (A) 9 LmASTISSnA L T/V/T/A|m|S\S\W 77.8 7 4 5 2 0 unbiased m:Oxidized methionine 62.32011 1.462652 1011.4786 995.487 15.9934 -1.5 1.7000000000000002 5.52 6838.6 nuORFdb_human ENST00000333775.9_1_17:7760068-7760422:- ENST00000333775.9_1_17:7760068-7760422:-|ENST00000575071.5_2_17:7760068-7760422:- NAA38 Out-of-Frame Out-of-Frame GN=NAA38 Out-of-Frame E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep1_Slot1-04_1_3496 40mio-rep3 E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep3_Slot2-5_1_3521.17786.461.1 1 1 1 11.79 6.27 11.79 74.9 7 0 0 3220.0 M17m Hydrogen 12 (A) 9 LTVTAmSSW (A) 9 L T/V/T/A|m\S\S\W 77.8 7 4 4 1 0 unbiased m:Oxidized methionine 61.441612 1.448999 1011.4806 995.487 15.9934 -1.5 3.0999999999999996 5.52 6838.6 nuORFdb_human ENST00000333775.9_1_17:7760068-7760422:- ENST00000333775.9_1_17:7760068-7760422:-|ENST00000575071.5_2_17:7760068-7760422:- NAA38 Out-of-Frame Out-of-Frame GN=NAA38 Out-of-Frame E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep3_Slot2-5_1_3521 40mio-rep4 E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep4_Slot2-5_1_3522.17766.451.1 1 1 1 8.21 6.71 8.21 66.0 7 0 0 3070.0 M17m Hydrogen 12 (A) 9 LTVTAmSSW (A) 9 L T/V/T/A|m\S\S\W 77.8 7 4 4 1 0 unbiased m:Oxidized methionine 61.354507 1.455282 1011.479 995.487 15.9934 -1.5 3.2 5.52 6838.6 nuORFdb_human ENST00000333775.9_1_17:7760068-7760422:- ENST00000333775.9_1_17:7760068-7760422:-|ENST00000575071.5_2_17:7760068-7760422:- NAA38 Out-of-Frame Out-of-Frame GN=NAA38 Out-of-Frame E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep4_Slot2-5_1_3522 20mio-rep3 E_20221201_NO30_400nL_HLAc1_2e7_directIP_titration_rep4_Slot2-4_1_3519.19460.1363.2 2 1 1 7.65 4.92 3.32 2.73 65.2 5 0 0 3200.0 Hydrogen 1 (-) 9 LVDVYIHYE (R) 9 VLDVNEnLHP L V/D|V|Y|I/H Y E 44.4 4 3 5 3 0 dual b/y bias 67.211037 0.899464 575.7881 1150.578 -0.0092 -8.0 -2.2 4.71 12048.3 nuORFdb_human T281045_5:36491432-36574985:- T281045_5:36491432-36574985:- TUCP Other GN=nan TUCP E_20221201_NO30_400nL_HLAc1_2e7_directIP_titration_rep4_Slot2-4_1_3519 40mio-rep1 E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep1_Slot1-04_1_3496.19689.1324.2 2 1 1 8.53 5.14 2.56 3.39 66.1 5 0 0 6880.0 Hydrogen 1 (-) 9 LVDVYIHYE (R) 9 VLDVNEnLHP L V/D|V|Y|I/H Y E 44.4 4 3 5 3 0 dual b/y bias 68.015831 0.923868 575.7906 1150.578 -0.0032 -2.8 0.40000000000000036 4.71 12048.3 nuORFdb_human T281045_5:36491432-36574985:- T281045_5:36491432-36574985:- TUCP Other GN=nan TUCP E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep1_Slot1-04_1_3496 40mio-rep2 E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep2_Slot2-5_1_3520.19391.1344.2 2 1 1 7.49 4.56 2.0 2.93 66.8 5 0 0 6190.0 Hydrogen 1 (-) 9 LVDVYIHYE (R) 9 VLDVnENLHP L V/D|V|Y|I/H Y E 44.4 4 3 5 3 0 dual b/y bias 66.986687 0.911393 575.7906 1150.578 -0.0032 -2.8 2.1000000000000005 4.71 12048.3 nuORFdb_human T281045_5:36491432-36574985:- T281045_5:36491432-36574985:- TUCP Other GN=nan TUCP E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep2_Slot2-5_1_3520 40mio-rep4 E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep4_Slot2-5_1_3522.21890.614.1 1 1 1 7.04 4.15 2.8 2.9 73.1 6 0 0 3810.0 Hydrogen 25 (P) 10 LVGPGGGVAL (T) 10 LGVGLPNGL L V G/P G/G\G\V\A\L 50.0 5 4 2 0 0 b-ion bias 75.600586 1.358045 839.4938 839.499 -0.0045 -5.4 -0.7000000000000002 5.52 8888.1 nuORFdb_human ENST00000523256.6_2_8:104427097-104427358:- ENST00000523256.6_2_8:104427097-104427358:- SLC25A32 5' Overlap uORF 5' Overlap uORF GN=SLC25A32 5' Overlap uORF E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep4_Slot2-5_1_3522 40mio-rep4 E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep4_Slot2-5_1_3522.25927.1388.2 2 1 1 7.24 5.09 1.08 2.15 56.0 6 0 0 4470.0 Hydrogen 28 (G) 9 LVPVSPAEL (S) 9 IVPSHKLM L/V/P\V\S\P A E\L 66.7 6 4 2 0 0 b-ion bias 89.551605 0.883798 462.7726 924.54 -0.0013 -1.4 3.3000000000000003 4.0 4803.5 nuORFdb_human T376737_X:78761331-78761463:- T376737_X:78761331-78761463:- lincRNA lncRNA GN=nan lincRNA E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep4_Slot2-5_1_3522 SCP E_20221201_NO30_400nL_HLAc1_4e7_PDAC_Slot2-6_1_3524.18537.316.1 1 2 1 13.55 6.42 7.6 7.14 89.4 7 0 0 66500.0 Hydrogen 9 (A) 9 LVTDLDTEY (-) 9 LnISLESEY L V/T/D\L\D\T\E\Y 77.8 7 5 2 0 0 b-ion bias 64.038818 1.534963 1068.5079 1068.509 0.0005 0.4 2.8 3.49 1835.0 smORF_v4|nuORFdb_human HERPUD2_7:35734044-35734097:- HERPUD2_7:35734044-35734097:-|ENST00000396081.5_1_7:35734046-35734373:-|ENST00000603731.1_2_7:35734046-35735162:-|ENST00000427455.1_2_7:35734046-35734534:-|ENST00000311350.7_1_7:35734046-35734675:- HERPUD2 uORF 7:35734044-35734097:- 7 HERPUD2 5' uORF 5' uORF GN=HERPUD2 E_20221201_NO30_400nL_HLAc1_4e7_PDAC_Slot2-6_1_3524 Exploris_FAIMS VH20221207_MKP_FAIMS2022E_HLAc1_4e7_PDAC.45371.45371.2 2 1 1 9.88 7.22 3.3 2.65 71.3 7 0 0 13900000.0 Hydrogen 9 (A) 9 LVTDLDTEY (-) 9 LSDTEVLEY L V\T\D\L\D-T-E/Y 77.8 7 4 1 0 2 b-ion bias 0.998 79.6 81.88 9.94 -70.0 534.7574 1068.509 -0.0019 -1.8 0.8 3.49 1835.0 smORF_v4|nuORFdb_human HERPUD2_7:35734044-35734097:- HERPUD2_7:35734044-35734097:-|ENST00000396081.5_1_7:35734046-35734373:-|ENST00000603731.1_2_7:35734046-35735162:-|ENST00000427455.1_2_7:35734046-35734534:-|ENST00000311350.7_1_7:35734046-35734675:- HERPUD2 uORF 7:35734044-35734097:- 7 HERPUD2 5' uORF 5' uORF GN=HERPUD2 VH20221207_MKP_FAIMS2022E_HLAc1_4e7_PDAC 40mio-rep1 E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep1_Slot1-04_1_3496.27733.351.1 1 1 1 13.04 6.71 7.38 6.34 82.8 7 0 0 13500.0 Hydrogen 26 (P) 9 LVWEVVESV (-) 9 IWnLVInSV L V/W/E|V\V\E\S\V 77.8 7 5 3 1 0 unbiased 95.809669 1.514419 1059.5689 1059.572 -0.0021 -2.0 1.2000000000000002 3.8 3971.6 nuORFdb_human ENST00000444563.5_1_1:43311951-43312056:- ENST00000444563.5_1_1:43311951-43312056:- RP11-342M1.3 Antisense lncRNA GN=RP11-342M1.3 Antisense E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep1_Slot1-04_1_3496 40mio-rep2 E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep2_Slot2-5_1_3520.27573.364.1 1 4 1 13.33 7.27 7.01 6.06 86.5 7 0 0 15100.0 Hydrogen 26 (P) 9 LVWEVVESV (-) 9 IWnLVInSV L V/W|E|V\V\E\S\V 77.8 7 6 3 2 0 b-ion bias 95.251686 1.506767 1059.5662 1059.572 -0.0021 -2.0 2.9000000000000004 3.8 3971.6 nuORFdb_human ENST00000444563.5_1_1:43311951-43312056:- ENST00000444563.5_1_1:43311951-43312056:- RP11-342M1.3 Antisense lncRNA GN=RP11-342M1.3 Antisense E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep2_Slot2-5_1_3520 40mio-rep3 E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep3_Slot2-5_1_3521.27586.1474.2 2 4 1 15.37 9.31 8.11 6.06 90.8 7 0 0 21100.0 Hydrogen 26 (P) 9 LVWEVVESV (-) 9 IVERnLESV L V/W\E-V|V|E\S\V 77.8 7 5 3 2 1 unbiased 95.289085 0.829838 530.2869 1059.572 -0.0054 -5.1 -0.5 3.8 3971.6 nuORFdb_human ENST00000444563.5_1_1:43311951-43312056:- ENST00000444563.5_1_1:43311951-43312056:- RP11-342M1.3 Antisense lncRNA GN=RP11-342M1.3 Antisense E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep3_Slot2-5_1_3521 40mio-rep4 E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep4_Slot2-5_1_3522.27584.1457.2 2 5 1 15.21 9.13 7.66 6.07 88.0 6 0 0 22700.0 Hydrogen 26 (P) 9 LVWEVVESV (-) 9 SVEDIREIV L V/W\E V|V|E\S\V 55.6 5 5 3 2 0 unbiased 95.274826 0.84067 530.2879 1059.572 -0.0034 -3.2 1.5 3.8 3971.6 nuORFdb_human ENST00000444563.5_1_1:43311951-43312056:- ENST00000444563.5_1_1:43311951-43312056:- RP11-342M1.3 Antisense lncRNA GN=RP11-342M1.3 Antisense E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep4_Slot2-5_1_3522 40mio-rep4 E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep4_Slot2-5_1_3522.29240.370.1 1 1 1 10.76 8.39 4.98 2.37 87.8 5 0 0 35500.0 Hydrogen 38 (F) 8 LWEAPLAW (R) 8 AEWIPLAW L W E\A|P\L|A\W 62.5 5 5 2 2 0 b-ion bias 100.993057 1.503068 985.5111 985.514 -0.0032 -3.2 1.5 4.0 7934.9 nuORFdb_human ENST00000589157.1_1_19:3542781-3542985:- ENST00000589157.1_1_19:3542781-3542985:- MFSD12 ncRNA Retained Intron lncRNA GN=MFSD12 ncRNA Retained Intron E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep4_Slot2-5_1_3522 Eva-50mio-3fr AL20210827_EV_HLA_PDAC_PANFRO413_2D_50e6_W632_classI_fxn2.30044.30044.2 2 1 1 12.85 4.26 8.71 8.59 65.6 8 0 1 13600000.0 Hydrogen 11 (E) 9 LWEEKPVCL (C) 9 APKGAATVWCL L/W|E/E/K/P/V/C/L 100.0 9 1 8 1 0 y-ion bias Carbamidomethylation C:Carbamidomethylation, 0.989 100.0 55.93 100.0 0.0 587.3026 1173.597 0.0007 0.6 0.3 4.53 3143.7 smORF_v4|nuORFdb_human NET1_10:5488602-5488681:+ NET1_10:5488602-5488681:+|ENST00000486354.1_1_10:5488601-5488679:+ NET1 uORF 10:5488602-5488681:+ 10 NET1 5' uORF 5' uORF GN=NET1 AL20210827_EV_HLA_PDAC_PANFRO413_2D_50e6_W632_classI_fxn2 Exploris AL20221201_MKP_PDAC_01.39480.39616.2 2 1 1 11.64 3.21 5.63 8.43 65.7 7 0 1 643000000.0 Hydrogen 11 (E) 9 LWEEKPVCL (C) 9 LWnISAVPSW L/W|E/E/K/P V/C/L 77.8 7 1 7 1 0 y-ion bias Carbamidomethylation C:Carbamidomethylation, 0.995 95.7 61.45 68.98 0.0 587.3025 1173.597 0.0005 0.5 -0.30000000000000004 4.53 3143.7 smORF_v4|nuORFdb_human NET1_10:5488602-5488681:+ NET1_10:5488602-5488681:+|ENST00000486354.1_1_10:5488601-5488679:+ NET1 uORF 10:5488602-5488681:+ 10 NET1 5' uORF 5' uORF GN=NET1 AL20221201_MKP_PDAC_01 SCP E_20221201_NO30_400nL_HLAc1_4e7_PDAC_Slot2-6_1_3524.18385.1355.2 2 8 1 18.95 10.57 8.38 8.38 96.6 7 0 1 50100.0 Hydrogen 11 (E) 9 LWEEKPVCL (C) 9 WLEAQEERL L/W|E|E|K|P V|C\L 77.8 7 6 6 5 0 dual b/y bias Carbamidomethylation C:Carbamidomethylation, 63.513966 0.90481 587.3011 1173.597 -0.0025 -2.2 0.19999999999999973 4.53 3143.7 smORF_v4|nuORFdb_human NET1_10:5488602-5488681:+ NET1_10:5488602-5488681:+|ENST00000486354.1_1_10:5488601-5488679:+ NET1 uORF 10:5488602-5488681:+ 10 NET1 5' uORF 5' uORF GN=NET1 E_20221201_NO30_400nL_HLAc1_4e7_PDAC_Slot2-6_1_3524 Exploris_FAIMS VH20221207_MKP_FAIMS2022E_HLAc1_4e7_PDAC.45669.45669.2 2 1 1 9.39 3.55 9.39 5.84 54.3 6 0 1 228000000.0 Hydrogen 11 (E) 9 LWEEKPVCL (C) 9 LWnISAVPSW L W|E/E/K/P V/C-L 55.6 5 1 5 1 1 y-ion bias Carbamidomethylation C:Carbamidomethylation, 0.874 89.2 82.4 4.82 -50.0 587.3007 1173.597 -0.0032 -2.7 -0.10000000000000009 4.53 3143.7 smORF_v4|nuORFdb_human NET1_10:5488602-5488681:+ NET1_10:5488602-5488681:+|ENST00000486354.1_1_10:5488601-5488679:+ NET1 uORF 10:5488602-5488681:+ 10 NET1 5' uORF 5' uORF GN=NET1 VH20221207_MKP_FAIMS2022E_HLAc1_4e7_PDAC SCP E_20221201_NO30_400nL_HLAc1_4e7_PDAC_Slot2-6_1_3524.30097.263.1 1 1 1 10.49 7.83 4.69 2.66 78.5 6 0 0 5560.0 Hydrogen 110 (S) 9 LWLPSLPSW (S) 9 ILWPSLPSW L W|L/P\S|L|P/S W 55.6 5 4 5 3 0 dual b/y bias 103.983444 1.566126 1098.5969 1098.598 0.0018 1.6 4.0 5.52 14708.2 nuORFdb_human ENST00000456708.5_1_2:220090211-220094280:- ENST00000456708.5_1_2:220090211-220094280:- ATG9A Nonsense Mediated Decay Other GN=ATG9A Nonsense Mediated Decay E_20221201_NO30_400nL_HLAc1_4e7_PDAC_Slot2-6_1_3524 SCP E_20221201_NO30_400nL_HLAc1_4e7_PDAC_Slot2-6_1_3524.18875.241.1 1 2 1 11.1 1.56 8.9 9.54 74.9 7 0 0 7690.0 Hydrogen 2 (M) 9 LYCTVTENF (-) 9 GTMFTEKMTT L Y/C|T\V|T\E\N-F 77.8 7 5 3 2 1 unbiased Carbamidomethylation C:Carbamidomethylation, 65.206741 1.578961 1146.5075 1146.514 -0.0036 -3.1 -0.7000000000000002 4.0 1277.5 nuORFdb_human ENST00000370207.4_1_1:94995956-94995989:- ENST00000370207.4_1_1:94995956-94995989:- F3 3' dORF 3' dORF GN=F3 3' dORF E_20221201_NO30_400nL_HLAc1_4e7_PDAC_Slot2-6_1_3524 Exploris_FAIMS VH20221207_MKP_FAIMS2022E_HLAc1_4e7_PDAC.46708.47006.2 2 1 1 7.05 2.87 7.05 4.18 60.1 6 0 0 32700000.0 Hydrogen 2 (M) 9 LYCTVTENF (-) 9 SIECTLYNF L Y\C\T-V T-E/N/F 55.6 5 2 2 0 2 unbiased Carbamidomethylation C:Carbamidomethylation, 0.994 99.6 83.93 18.34 -70.0 573.759 1146.514 -0.0029 -2.5 0.10000000000000009 4.0 1277.5 nuORFdb_human ENST00000370207.4_1_1:94995956-94995989:- ENST00000370207.4_1_1:94995956-94995989:- F3 3' dORF 3' dORF GN=F3 3' dORF VH20221207_MKP_FAIMS2022E_HLAc1_4e7_PDAC Eva-50mio-3fr AL20210827_EV_HLA_PDAC_PANFRO413_2D_50e6_W632_classI_fxn3.44364.44364.2 2 1 1 11.35 4.1 5.75 7.25 76.1 7 0 0 53200000.0 Hydrogen 20 (M) 9 LYLPTLTHF (S) 9 LYLPTEGRR L Y|L/P/T/L/T/H-F 77.8 7 1 6 1 1 y-ion bias 0.945 95.4 80.28 13.74 0.0 552.8084 1104.609 0.0008 0.7 0.19999999999999996 6.74 4605.4 nuORFdb_human ENST00000537473.2_1_12:57058103-57058217:- ENST00000537473.2_1_12:57058103-57058217:- PTGES3 3' dORF 3' dORF GN=PTGES3 3' dORF AL20210827_EV_HLA_PDAC_PANFRO413_2D_50e6_W632_classI_fxn3 Exploris AL20221201_MKP_PDAC_01.63648.63648.2 2 2 1 13.76 6.4 5.83 7.36 83.9 7 0 0 442000000.0 Hydrogen 20 (M) 9 LYLPTLTHF (S) 9 LYLKSKDTH L Y|L/P/T/L/T/H/F 77.8 7 1 7 1 0 y-ion bias 0.748 86.0 96.68 2.71 0.0 552.8093 1104.609 0.0024 2.2 1.4000000000000001 6.74 4605.4 nuORFdb_human ENST00000537473.2_1_12:57058103-57058217:- ENST00000537473.2_1_12:57058103-57058217:- PTGES3 3' dORF 3' dORF GN=PTGES3 3' dORF AL20221201_MKP_PDAC_01 SCP E_20221201_NO30_400nL_HLAc1_4e7_PDAC_Slot2-6_1_3524.27568.1176.2 2 1 1 15.33 7.59 7.79 7.74 90.8 9 2 2 33500.0 Hydrogen 5 (F) 11 LYPKSEAFRLF (F) 11 GIINPSAFYIYL L/Y|P/K/S/E|A|F R\L\F 81.8 9 5 7 3 0 unbiased 95.243172 1.015514 685.8756 1370.747 -0.002 -1.4 1.0 8.59 7314.6 nuORFdb_human T313091_6:145246178-145246364:+ T313091_6:145246178-145246364:+ lincRNA lncRNA GN=nan lincRNA E_20221201_NO30_400nL_HLAc1_4e7_PDAC_Slot2-6_1_3524 Exploris AL20221201_MKP_PDAC_01.61995.61995.3 3 1 1 10.18 6.9 2.41 3.28 64.8 5 1 1 4910000.0 Hydrogen 46 (V) 9 LYQKHFLLL (L) 9 YQKHFLLLL L Y\Q K H\F\L\L/L 44.4 4 4 1 0 0 b-ion bias 0.939 100.0 93.68 21.54 0.0 392.239 1174.698 0.004 3.4 2.5999999999999996 8.6 6865.4 smORF_v4|nuORFdb_human CALM2_2:47387405-47387581:- CALM2_2:47387405-47387581:-|ENST00000272298.11_1_2:47387407-47387581:- CALM2 dORF 2:47387405-47387581:- 2 CALM2 3' dORF 3' dORF GN=CALM2 AL20221201_MKP_PDAC_01 SCP E_20221201_NO30_400nL_HLAc1_4e7_PDAC_Slot2-6_1_3524.27824.1260.2 2 1 1 18.56 11.92 6.64 6.64 89.6 8 1 1 9990.0 Hydrogen 46 (V) 9 LYQKHFLLL (L) 9 LEVAAWFILL L/Y|Q|K/H|F\L|L\L 100.0 9 6 6 4 0 dual b/y bias 96.127113 0.963577 587.8536 1174.698 0.0024 2.1 4.5 8.6 6865.4 smORF_v4|nuORFdb_human CALM2_2:47387405-47387581:- CALM2_2:47387405-47387581:-|ENST00000272298.11_1_2:47387407-47387581:- CALM2 dORF 2:47387405-47387581:- 2 CALM2 3' dORF 3' dORF GN=CALM2 E_20221201_NO30_400nL_HLAc1_4e7_PDAC_Slot2-6_1_3524 SCP E_20221201_NO30_400nL_HLAc1_4e7_PDAC_Slot2-6_1_3524.29857.152.1 1 1 1 10.56 5.94 7.28 4.62 73.4 6 0 0 14400.0 Hydrogen 52 (K) 9 LYSDIFWDL (V) 9 LDIYSFWLn L Y/S D|I|F\W\D\L 55.6 5 5 3 2 0 unbiased 103.154091 1.631207 1171.5639 1171.567 -0.007 -6.0 -3.6 3.56 7615.7 nuORFdb_human ENST00000319933.6_1_5:102594502-102611616:+ ENST00000319933.6_1_5:102594502-102611616:+|ENST00000515669.5_1_5:102594747-102611616:+|ENST00000510890.1_1_5:102595280-102611616:+ C5orf30 5' uORF 5' uORF GN=C5orf30 5' uORF E_20221201_NO30_400nL_HLAc1_4e7_PDAC_Slot2-6_1_3524 SCP E_20221201_NO30_400nL_HLAc1_4e7_PDAC_Slot2-6_1_3524.15473.1217.2 2 1 1 12.0 5.84 5.94 6.16 71.8 6 0 0 7450.0 Hydrogen 10 (A) 11 LYSHQMSQMNF (Q) 11 SMREVDEQMFN L Y S H Q|M\S/Q|M|N\F 54.5 6 5 4 3 0 unbiased 53.455883 0.990166 693.3003 1385.598 -0.0049 -3.6 -1.2000000000000002 6.74 3831.4 nuORFdb_human ENST00000523619.5_1_8:18730156-18744454:- ENST00000523619.5_1_8:18730156-18744454:-|ENST00000327040.12_1_8:18730156-18793627:- PSD3 5' Overlap uORF 5' Overlap uORF GN=PSD3 5' Overlap uORF E_20221201_NO30_400nL_HLAc1_4e7_PDAC_Slot2-6_1_3524 Exploris_FAIMS VH20221207_MKP_FAIMS2022E_HLAc1_4e7_PDAC.47479.47479.2 2 1 1 11.44 5.36 7.47 6.08 79.2 7 1 1 127000000.0 Hydrogen 29 (Q) 9 LYTDVKVSF (N) 9 LnTYLTKAF L Y|T-D/V/K-V/S/F 77.8 7 1 5 1 2 y-ion bias 0.925 83.9 84.62 18.03 -50.0 536.2896 1071.572 -0.0003 -0.2 2.4 5.83 8921.6 nuORFdb_human T381097_X:143380150-143681817:- T381097_X:143380150-143681817:- lincRNA lncRNA GN=nan lincRNA VH20221207_MKP_FAIMS2022E_HLAc1_4e7_PDAC Eva-50mio-3fr AL20210827_EV_HLA_PDAC_PANFRO413_2D_50e6_W632_classI_fxn3.27396.27497.3 3 2 2 10.56 10.56 10.56 73.1 7 1 1 259000000.0 Hydrogen 171 (S) 9 LYTFIKHEF (C) 9 L/Y/T/F/I/K/H E/F 77.8 7 0 7 0 0 y-ion bias 0.994 97.0 50.73 21.33 0.0 399.8816 1197.63 -0.0001 -0.1 -0.6 6.75 21094.8 nuORFdb_human ENST00000512410.1_1_4:175416602-175443578:- ENST00000512410.1_1_4:175416602-175443578:- HPGD 3' Overlap dORF 3' Overlap dORF GN=HPGD 3' Overlap dORF AL20210827_EV_HLA_PDAC_PANFRO413_2D_50e6_W632_classI_fxn3 Exploris AL20221201_MKP_PDAC_01.35888.35888.3 3 1 1 9.87 9.87 9.87 65.9 6 1 1 34100000.0 Hydrogen 171 (S) 9 LYTFIKHEF (C) 9 L Y/T/F/I/K/H E/F 55.6 5 0 6 0 0 y-ion bias 0.777 94.7 56.13 19.74 0.0 399.8824 1197.63 0.0025 2.1 1.3 6.75 21094.8 nuORFdb_human ENST00000512410.1_1_4:175416602-175443578:- ENST00000512410.1_1_4:175416602-175443578:- HPGD 3' Overlap dORF 3' Overlap dORF GN=HPGD 3' Overlap dORF AL20221201_MKP_PDAC_01 SCP E_20221201_NO30_400nL_HLAc1_4e7_PDAC_Slot2-6_1_3524.24521.1262.2 2 14 1 18.5 6.43 12.71 12.07 91.9 8 1 1 156000.0 Hydrogen 171 (S) 9 LYTFIKHEF (C) 9 IYTTKWSGLE L/Y|T|F|I|K/H|E\F 100.0 9 6 7 5 0 dual b/y bias 84.712944 0.962351 599.3195 1197.63 0.0025 2.0 4.4 6.75 21094.8 nuORFdb_human ENST00000512410.1_1_4:175416602-175443578:- ENST00000512410.1_1_4:175416602-175443578:- HPGD 3' Overlap dORF 3' Overlap dORF GN=HPGD 3' Overlap dORF E_20221201_NO30_400nL_HLAc1_4e7_PDAC_Slot2-6_1_3524 Eva-50mio-3fr AL20210827_EV_HLA_PDAC_PANFRO413_2D_50e6_W632_classI_fxn3.36939.36939.2 2 1 1 12.45 4.66 9.01 7.8 83.2 6 1 1 14900000.0 Hydrogen 375 (N) 9 LYVGKIPGF (C) 9 IYVGLSRVS L Y|V/G|K|I/P G/F 55.6 5 3 6 3 0 dual b/y bias 0.992 91.9 67.33 100.0 0.0 497.2923 993.577 0.0006 0.6 0.09999999999999998 8.59 42662.9 nuORFdb_human ENST00000488620.5_1_9:116327559-116353744:+ ENST00000488620.5_1_9:116327559-116353744:+ RGS3 5' Overlap uORF 5' Overlap uORF GN=RGS3 5' Overlap uORF AL20210827_EV_HLA_PDAC_PANFRO413_2D_50e6_W632_classI_fxn3 Exploris AL20221201_MKP_PDAC_01.49813.49813.2 2 1 1 9.17 0.83 6.67 8.34 63.2 6 1 1 4640000.0 Hydrogen 375 (N) 9 LYVGKIPGF (C) 9 IGGVKIYFP L Y|V/G|K|I/P G/F 55.6 5 3 6 3 0 dual b/y bias 0.525 70.3 76.45 100.0 0.0 497.2918 993.577 -0.0005 -0.5 -1.3 8.59 42662.9 nuORFdb_human ENST00000488620.5_1_9:116327559-116353744:+ ENST00000488620.5_1_9:116327559-116353744:+ RGS3 5' Overlap uORF 5' Overlap uORF GN=RGS3 5' Overlap uORF AL20221201_MKP_PDAC_01 10mio-rep2 E_20221201_NO30_400nL_HLAc1_1e7_directIP_titration_rep2_Slot2-3_1_3513.18601.485.1 1 1 1 7.74 6.03 1.71 1.71 75.1 5 0 0 2290.0 Hydrogen 1 (-) 11 MAAAAAAAAAW (A) 11 MAAAAAAAAEQ M A A A A A\A\A\A\A\W 45.5 5 5 0 0 0 b-ion bias 64.251724 1.434669 975.469 975.472 -0.0027 -2.7 4.0 5.27 1591.8 smORF_v4|nuORFdb_human PRELID1_5:176730817-176730870:+ PRELID1_5:176730817-176730870:+|ENST00000510797.1_1_5:176730816-176730870:+ PRELID1 uORF 5:176730817-176730870:+ 5 PRELID1 5' uORF 5' uORF GN=PRELID1 E_20221201_NO30_400nL_HLAc1_1e7_directIP_titration_rep2_Slot2-3_1_3513 20mio-rep2 E_20221201_NO30_400nL_HLAc1_2e7_directIP_titration_rep3_Slot2-4_1_3518.18622.1574.2 2 3 1 13.2 5.76 5.3 7.44 86.2 6 0 0 10300.0 Hydrogen 1 (-) 11 MAAAAAAAAAW (A) 11 RSSSGPGAWA M A A\A A\A A|A|A|A\W 36.4 4 6 3 3 0 b-ion bias 64.321213 0.767034 488.2352 975.472 -0.0089 -9.2 -2.8999999999999995 5.27 1591.8 smORF_v4|nuORFdb_human PRELID1_5:176730817-176730870:+ PRELID1_5:176730817-176730870:+|ENST00000510797.1_1_5:176730816-176730870:+ PRELID1 uORF 5:176730817-176730870:+ 5 PRELID1 5' uORF 5' uORF GN=PRELID1 E_20221201_NO30_400nL_HLAc1_2e7_directIP_titration_rep3_Slot2-4_1_3518 20mio-rep3 E_20221201_NO30_400nL_HLAc1_2e7_directIP_titration_rep4_Slot2-4_1_3519.18580.1556.2 2 2 1 9.33 4.72 3.56 4.61 66.6 5 0 0 6070.0 Hydrogen 1 (-) 11 MAAAAAAAAAW (A) 11 ALCLGNAAGE M A A A A|A A\A\A|A\W 36.4 4 5 2 2 0 b-ion bias 64.172447 0.778104 488.2364 975.472 -0.0069 -7.1 -1.2999999999999998 5.27 1591.8 smORF_v4|nuORFdb_human PRELID1_5:176730817-176730870:+ PRELID1_5:176730817-176730870:+|ENST00000510797.1_1_5:176730816-176730870:+ PRELID1 uORF 5:176730817-176730870:+ 5 PRELID1 5' uORF 5' uORF GN=PRELID1 E_20221201_NO30_400nL_HLAc1_2e7_directIP_titration_rep4_Slot2-4_1_3519 SCP E_20221201_NO30_400nL_HLAc1_4e7_PDAC_Slot2-6_1_3524.18322.462.1 1 2 1 10.39 3.03 5.85 7.36 81.3 7 0 0 7560.0 Hydrogen 1 (-) 11 MAAAAAAAAAW (A) 11 TDRAGGAAGSL M A A A\A\A\A\A\A\A\W 63.6 7 7 0 0 0 b-ion bias 63.296436 1.448793 975.4717 975.472 0.0003 0.3 2.6999999999999997 5.27 1591.8 smORF_v4|nuORFdb_human PRELID1_5:176730817-176730870:+ PRELID1_5:176730817-176730870:+|ENST00000510797.1_1_5:176730816-176730870:+ PRELID1 uORF 5:176730817-176730870:+ 5 PRELID1 5' uORF 5' uORF GN=PRELID1 E_20221201_NO30_400nL_HLAc1_4e7_PDAC_Slot2-6_1_3524 40mio-rep1 E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep1_Slot1-04_1_3496.18768.451.1 1 3 1 12.52 10.45 2.08 2.08 86.3 8 0 0 57600.0 Hydrogen 1 (-) 11 MAAAAAAAAAW (A) 11 MAAAAAAAAEQ M A A\A\A\A\A\A\A\A\W 72.7 8 8 0 0 0 b-ion bias 64.834282 1.45514 975.4683 975.472 -0.0037 -3.8 -0.5999999999999996 5.27 1591.8 smORF_v4|nuORFdb_human PRELID1_5:176730817-176730870:+ PRELID1_5:176730817-176730870:+|ENST00000510797.1_1_5:176730816-176730870:+ PRELID1 uORF 5:176730817-176730870:+ 5 PRELID1 5' uORF 5' uORF GN=PRELID1 E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep1_Slot1-04_1_3496 40mio-rep2 E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep2_Slot2-5_1_3520.18451.466.1 1 3 1 11.01 8.89 2.12 2.12 84.7 7 0 0 15300.0 Hydrogen 1 (-) 11 MAAAAAAAAAW (A) 11 MAAAAAAAAEQ M A A A\A\A\A\A\A\A\W 63.6 7 7 0 0 0 b-ion bias 63.739754 1.44618 975.4701 975.472 -0.0017 -1.7 3.2 5.27 1591.8 smORF_v4|nuORFdb_human PRELID1_5:176730817-176730870:+ PRELID1_5:176730817-176730870:+|ENST00000510797.1_1_5:176730816-176730870:+ PRELID1 uORF 5:176730817-176730870:+ 5 PRELID1 5' uORF 5' uORF GN=PRELID1 E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep2_Slot2-5_1_3520 40mio-rep3 E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep3_Slot2-5_1_3521.18501.1550.2 2 5 1 11.86 6.83 5.17 5.03 83.3 5 0 0 22900.0 Hydrogen 1 (-) 11 MAAAAAAAAAW (A) 11 ALCLGNAAGE M A A A\A A A|A|A\A\W 36.4 4 5 2 2 0 b-ion bias 63.910542 0.781952 488.2366 975.472 -0.0049 -5.1 -0.5 5.27 1591.8 smORF_v4|nuORFdb_human PRELID1_5:176730817-176730870:+ PRELID1_5:176730817-176730870:+|ENST00000510797.1_1_5:176730816-176730870:+ PRELID1 uORF 5:176730817-176730870:+ 5 PRELID1 5' uORF 5' uORF GN=PRELID1 E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep3_Slot2-5_1_3521 40mio-rep4 E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep4_Slot2-5_1_3522.18476.467.1 1 5 1 10.09 8.06 2.03 2.03 83.4 7 0 0 18200.0 Hydrogen 1 (-) 11 MAAAAAAAAAW (A) 11 MAAAAAAAAEQ M A A A\A\A\A\A\A\A\W 63.6 7 7 0 0 0 b-ion bias 63.807629 1.445435 975.4666 975.472 -0.0047 -4.8 -0.09999999999999964 5.27 1591.8 smORF_v4|nuORFdb_human PRELID1_5:176730817-176730870:+ PRELID1_5:176730817-176730870:+|ENST00000510797.1_1_5:176730816-176730870:+ PRELID1 uORF 5:176730817-176730870:+ 5 PRELID1 5' uORF 5' uORF GN=PRELID1 E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep4_Slot2-5_1_3522 5mio-rep1 E_20221201_NO30_400nL_HLAc1_5e6_directIP_titration_rep1_Slot1-37_1_3502.18882.473.1 1 1 1 10.72 9.32 1.4 1.4 84.6 7 0 0 3810.0 Hydrogen 1 (-) 11 MAAAAAAAAAW (A) 11 MAAAAAAAAEQ M A A\A\A A\A\A\A\A\W 54.5 6 7 0 0 0 b-ion bias 65.218483 1.44187 975.4643 975.472 -0.0077 -7.9 -0.9000000000000004 5.27 1591.8 smORF_v4|nuORFdb_human PRELID1_5:176730817-176730870:+ PRELID1_5:176730817-176730870:+|ENST00000510797.1_1_5:176730816-176730870:+ PRELID1 uORF 5:176730817-176730870:+ 5 PRELID1 5' uORF 5' uORF GN=PRELID1 E_20221201_NO30_400nL_HLAc1_5e6_directIP_titration_rep1_Slot1-37_1_3502 10mio-rep1 E_20221201_NO30_400nL_HLAc1_1e7_directIP_titration_rep1_Slot2-3_1_3512.15244.458.1 1 5 1 11.94 9.33 2.61 2.61 81.2 8 0 0 15100.0 M1m Hydrogen 1 (-) 11 mAAAAAAAAAW (A) 11 mAAAAAAAAEQ m A A\A\A|A\A\A\A\A\W 72.7 8 8 1 1 0 b-ion bias m:Oxidized methionine 52.661694 1.451173 991.4609 975.472 15.9893 -5.6 2.1000000000000005 5.27 1591.8 smORF_v4|nuORFdb_human PRELID1_5:176730817-176730870:+ PRELID1_5:176730817-176730870:+|ENST00000510797.1_1_5:176730816-176730870:+ PRELID1 uORF 5:176730817-176730870:+ 5 PRELID1 5' uORF 5' uORF GN=PRELID1 E_20221201_NO30_400nL_HLAc1_1e7_directIP_titration_rep1_Slot2-3_1_3512 10mio-rep2 E_20221201_NO30_400nL_HLAc1_1e7_directIP_titration_rep2_Slot2-3_1_3513.15202.1557.2 2 2 1 10.89 7.03 3.94 3.85 77.8 7 0 0 6940.0 M1m Hydrogen 1 (-) 11 mAAAAAAAAAW (A) 11 GYAPGGAAAAAn m A A/A A\A\A|A\A|A\W 54.5 6 6 3 2 0 b-ion bias m:Oxidized methionine 52.514511 0.777324 496.234 975.472 15.9891 -5.9 0.7999999999999998 5.27 1591.8 smORF_v4|nuORFdb_human PRELID1_5:176730817-176730870:+ PRELID1_5:176730817-176730870:+|ENST00000510797.1_1_5:176730816-176730870:+ PRELID1 uORF 5:176730817-176730870:+ 5 PRELID1 5' uORF 5' uORF GN=PRELID1 E_20221201_NO30_400nL_HLAc1_1e7_directIP_titration_rep2_Slot2-3_1_3513 10mio-rep3 E_20221201_NO30_400nL_HLAc1_1e7_directIP_titration_rep3_Slot2-3_1_3514.15167.1548.2 2 1 1 8.59 6.34 3.08 2.26 69.2 6 0 0 5000.0 M1m Hydrogen 1 (-) 11 mAAAAAAAAAW (A) 11 GYAPGGAAAAAn m A/A A A A\A\A\A|A\W 45.5 5 5 2 1 0 b-ion bias m:Oxidized methionine 52.396011 0.783509 496.2346 975.472 15.9911 -3.9 2.4 5.27 1591.8 smORF_v4|nuORFdb_human PRELID1_5:176730817-176730870:+ PRELID1_5:176730817-176730870:+|ENST00000510797.1_1_5:176730816-176730870:+ PRELID1 uORF 5:176730817-176730870:+ 5 PRELID1 5' uORF 5' uORF GN=PRELID1 E_20221201_NO30_400nL_HLAc1_1e7_directIP_titration_rep3_Slot2-3_1_3514 20mio-rep2 E_20221201_NO30_400nL_HLAc1_2e7_directIP_titration_rep3_Slot2-4_1_3518.15300.1559.2 2 4 1 14.0 8.0 7.05 6.0 85.1 7 0 0 22100.0 M1m Hydrogen 1 (-) 11 mAAAAAAAAAW (A) 11 GYAPGGAAAAAn m A A/A A\A\A|A|A|A\W 54.5 6 6 4 3 0 unbiased m:Oxidized methionine 52.84679 0.776234 496.2327 975.472 15.9871 -7.9 -1.6000000000000005 5.27 1591.8 smORF_v4|nuORFdb_human PRELID1_5:176730817-176730870:+ PRELID1_5:176730817-176730870:+|ENST00000510797.1_1_5:176730816-176730870:+ PRELID1 uORF 5:176730817-176730870:+ 5 PRELID1 5' uORF 5' uORF GN=PRELID1 E_20221201_NO30_400nL_HLAc1_2e7_directIP_titration_rep3_Slot2-4_1_3518 20mio-rep3 E_20221201_NO30_400nL_HLAc1_2e7_directIP_titration_rep4_Slot2-4_1_3519.15174.1534.2 2 5 1 12.73 6.7 4.47 6.03 78.7 8 0 0 26700.0 M1m Hydrogen 1 (-) 11 mAAAAAAAAAW (A) 11 GYAPGGAAAAAn m A A/A/A\A\A|A|A|A\W 72.7 8 6 5 3 0 unbiased m:Oxidized methionine 52.413517 0.792278 496.2334 975.472 15.9871 -7.9 -2.1000000000000005 5.27 1591.8 smORF_v4|nuORFdb_human PRELID1_5:176730817-176730870:+ PRELID1_5:176730817-176730870:+|ENST00000510797.1_1_5:176730816-176730870:+ PRELID1 uORF 5:176730817-176730870:+ 5 PRELID1 5' uORF 5' uORF GN=PRELID1 E_20221201_NO30_400nL_HLAc1_2e7_directIP_titration_rep4_Slot2-4_1_3519 40mio-rep1 E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep1_Slot1-04_1_3496.15405.426.1 1 2 1 11.73 7.89 3.84 3.84 80.6 7 0 0 17800.0 M1m Hydrogen 1 (-) 11 mAAAAAAAAAW (A) 11 mAAAAAAAAEQ m A A A|A\A\A\A\A\A\W 63.6 7 7 1 1 0 b-ion bias m:Oxidized methionine 53.213669 1.469764 991.4645 975.472 15.9923 -2.6 0.6000000000000001 5.27 1591.8 smORF_v4|nuORFdb_human PRELID1_5:176730817-176730870:+ PRELID1_5:176730817-176730870:+|ENST00000510797.1_1_5:176730816-176730870:+ PRELID1 uORF 5:176730817-176730870:+ 5 PRELID1 5' uORF 5' uORF GN=PRELID1 E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep1_Slot1-04_1_3496 40mio-rep2 E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep2_Slot2-5_1_3520.15195.445.1 1 4 1 11.01 7.3 3.71 3.71 79.0 7 0 0 21000.0 M1m Hydrogen 1 (-) 11 mAAAAAAAAAW (A) 11 mAAAAAAAAEQ m A A A|A|A\A\A\A\A\W 63.6 7 7 2 2 0 b-ion bias m:Oxidized methionine 52.489819 1.458788 991.4632 975.472 15.9913 -3.6 1.3000000000000003 5.27 1591.8 smORF_v4|nuORFdb_human PRELID1_5:176730817-176730870:+ PRELID1_5:176730817-176730870:+|ENST00000510797.1_1_5:176730816-176730870:+ PRELID1 uORF 5:176730817-176730870:+ 5 PRELID1 5' uORF 5' uORF GN=PRELID1 E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep2_Slot2-5_1_3520 40mio-rep3 E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep3_Slot2-5_1_3521.15195.453.1 1 3 1 9.71 7.07 2.64 2.64 75.3 7 0 0 19100.0 M1m Hydrogen 1 (-) 11 mAAAAAAAAAW (A) 11 mAAAAAAAAEQ m A A A\A|A\A\A\A\A\W 63.6 7 7 1 1 0 b-ion bias m:Oxidized methionine 52.491234 1.453779 991.4615 975.472 15.9893 -5.6 -1.0 5.27 1591.8 smORF_v4|nuORFdb_human PRELID1_5:176730817-176730870:+ PRELID1_5:176730817-176730870:+|ENST00000510797.1_1_5:176730816-176730870:+ PRELID1 uORF 5:176730817-176730870:+ 5 PRELID1 5' uORF 5' uORF GN=PRELID1 E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep3_Slot2-5_1_3521 40mio-rep4 E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep4_Slot2-5_1_3522.15126.1529.2 2 4 1 10.08 5.58 3.64 4.5 73.0 6 0 0 25500.0 M1m Hydrogen 1 (-) 11 mAAAAAAAAAW (A) 11 PTAFNNAAW m A A A A\A\A|A\A|A\W 54.5 6 6 2 2 0 b-ion bias m:Oxidized methionine 52.238457 0.794956 496.236 975.472 15.9931 -1.9 2.8000000000000003 5.27 1591.8 smORF_v4|nuORFdb_human PRELID1_5:176730817-176730870:+ PRELID1_5:176730817-176730870:+|ENST00000510797.1_1_5:176730816-176730870:+ PRELID1 uORF 5:176730817-176730870:+ 5 PRELID1 5' uORF 5' uORF GN=PRELID1 E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep4_Slot2-5_1_3522 5mio-rep1 E_20221201_NO30_400nL_HLAc1_5e6_directIP_titration_rep1_Slot1-37_1_3502.15500.448.1 1 1 1 7.9 6.11 1.79 1.79 75.6 6 0 0 1950.0 M1m Hydrogen 1 (-) 11 mAAAAAAAAAW (A) 11 mAAAAAAAAEQ m A A A A|A\A\A\A\A\W 54.5 6 6 1 1 0 b-ion bias m:Oxidized methionine 53.542603 1.457041 991.4576 975.472 15.9863 -8.7 -1.6999999999999993 5.27 1591.8 smORF_v4|nuORFdb_human PRELID1_5:176730817-176730870:+ PRELID1_5:176730817-176730870:+|ENST00000510797.1_1_5:176730816-176730870:+ PRELID1 uORF 5:176730817-176730870:+ 5 PRELID1 5' uORF 5' uORF GN=PRELID1 E_20221201_NO30_400nL_HLAc1_5e6_directIP_titration_rep1_Slot1-37_1_3502 40mio-rep1 E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep1_Slot1-04_1_3496.13629.1334.2 2 1 1 16.38 1.63 12.32 14.75 84.8 9 1 1 9870.0 Hydrogen 1 (-) 10 MAFNSQTRTF (R) 10 SDFHPALNQSS M/A/F/N|S|Q|T|R\T\F 100.0 10 6 7 4 0 dual b/y bias 47.078938 0.91791 601.7834 1202.562 -0.0035 -2.9 0.30000000000000027 9.5 3042.6 smORF_v4|nuORFdb_human LOC101928979_1:145382210-145382290:+ LOC101928979_1:145382210-145382290:+|ENST00000611275.4_2_1:145382212-145382344:-|T020086_1:149224691-149224769:+ LOC101928979 lncRNA 1:145382210-145382290:+ 1 LINC01719 lincRNA lncRNA GN=LOC101928979 E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep1_Slot1-04_1_3496 40mio-rep3 E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep3_Slot2-5_1_3521.13349.1359.2 2 2 1 8.78 8.78 8.78 60.4 8 1 1 14200.0 Hydrogen 1 (-) 10 MAFNSQTRTF (R) 10 M/A/F|N/S Q|T/R\T\F 80.0 8 4 6 2 0 unbiased 46.117989 0.901979 601.7821 1202.562 -0.0055 -4.6 0.0 9.5 3042.6 smORF_v4|nuORFdb_human LOC101928979_1:145382210-145382290:+ LOC101928979_1:145382210-145382290:+|ENST00000611275.4_2_1:145382212-145382344:-|T020086_1:149224691-149224769:+ LOC101928979 lncRNA 1:145382210-145382290:+ 1 LINC01719 lincRNA lncRNA GN=LOC101928979 E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep3_Slot2-5_1_3521 40mio-rep4 E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep4_Slot2-5_1_3522.13341.1353.2 2 1 1 9.02 9.02 9.02 63.7 9 1 1 7700.0 Hydrogen 1 (-) 10 MAFNSQTRTF (R) 10 M/A/F|N/S|Q/T/R|T\F 100.0 10 4 8 3 0 y-ion bias 46.071339 0.90552 601.7829 1202.562 -0.0035 -2.9 1.8000000000000003 9.5 3042.6 smORF_v4|nuORFdb_human LOC101928979_1:145382210-145382290:+ LOC101928979_1:145382210-145382290:+|ENST00000611275.4_2_1:145382212-145382344:-|T020086_1:149224691-149224769:+ LOC101928979 lncRNA 1:145382210-145382290:+ 1 LINC01719 lincRNA lncRNA GN=LOC101928979 E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep4_Slot2-5_1_3522 20mio-rep1 E_20221201_NO30_400nL_HLAc1_2e7_directIP_titration_rep1_Slot2-4_1_3515.10082.1373.2 2 1 1 12.87 12.87 12.87 66.9 8 1 1 7370.0 M1m Hydrogen 1 (-) 10 mAFNSQTRTF (R) 10 m A/F|N|S|Q/T|R\T\F 80.0 8 6 6 4 0 dual b/y bias m:Oxidized methionine 34.824234 0.893274 609.777 1202.562 15.9845 -8.6 -2.3999999999999995 9.5 3042.6 smORF_v4|nuORFdb_human LOC101928979_1:145382210-145382290:+ LOC101928979_1:145382210-145382290:+|ENST00000611275.4_2_1:145382212-145382344:-|T020086_1:149224691-149224769:+ LOC101928979 lncRNA 1:145382210-145382290:+ 1 LINC01719 lincRNA lncRNA GN=LOC101928979 E_20221201_NO30_400nL_HLAc1_2e7_directIP_titration_rep1_Slot2-4_1_3515 20mio-rep2 E_20221201_NO30_400nL_HLAc1_2e7_directIP_titration_rep3_Slot2-4_1_3518.10239.1361.2 2 2 1 9.05 9.05 9.05 62.5 8 1 1 10900.0 M1m Hydrogen 1 (-) 10 mAFNSQTRTF (R) 10 m A/F/N/S/Q|T/R\T\F 80.0 8 3 6 1 0 y-ion bias m:Oxidized methionine 35.368244 0.900975 609.7801 1202.562 15.9905 -3.7 2.5999999999999996 9.5 3042.6 smORF_v4|nuORFdb_human LOC101928979_1:145382210-145382290:+ LOC101928979_1:145382210-145382290:+|ENST00000611275.4_2_1:145382212-145382344:-|T020086_1:149224691-149224769:+ LOC101928979 lncRNA 1:145382210-145382290:+ 1 LINC01719 lincRNA lncRNA GN=LOC101928979 E_20221201_NO30_400nL_HLAc1_2e7_directIP_titration_rep3_Slot2-4_1_3518 40mio-rep1 E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep1_Slot1-04_1_3496.10377.1340.2 2 1 1 9.94 9.94 9.94 58.3 8 1 1 18800.0 M1m Hydrogen 1 (-) 10 mAFNSQTRTF (R) 10 m A/F|N|S/Q/T/R\T\F 80.0 8 4 6 2 0 unbiased m:Oxidized methionine 35.848038 0.913764 609.7798 1202.562 15.9905 -3.7 -0.5 9.5 3042.6 smORF_v4|nuORFdb_human LOC101928979_1:145382210-145382290:+ LOC101928979_1:145382210-145382290:+|ENST00000611275.4_2_1:145382212-145382344:-|T020086_1:149224691-149224769:+ LOC101928979 lncRNA 1:145382210-145382290:+ 1 LINC01719 lincRNA lncRNA GN=LOC101928979 E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep1_Slot1-04_1_3496 40mio-rep2 E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep2_Slot2-5_1_3520.10093.1354.2 2 1 1 9.21 9.21 9.21 61.4 8 1 1 11200.0 M1m Hydrogen 1 (-) 10 mAFNSQTRTF (R) 10 m A/F|N|S/Q/T/R\T\F 80.0 8 4 6 2 0 unbiased m:Oxidized methionine 34.866108 0.904912 609.7782 1202.562 15.9865 -6.9 -2.0 9.5 3042.6 smORF_v4|nuORFdb_human LOC101928979_1:145382210-145382290:+ LOC101928979_1:145382210-145382290:+|ENST00000611275.4_2_1:145382212-145382344:-|T020086_1:149224691-149224769:+ LOC101928979 lncRNA 1:145382210-145382290:+ 1 LINC01719 lincRNA lncRNA GN=LOC101928979 E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep2_Slot2-5_1_3520 40mio-rep3 E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep3_Slot2-5_1_3521.10091.1356.2 2 1 1 10.87 10.87 10.87 67.6 8 1 1 11700.0 M1m Hydrogen 1 (-) 10 mAFNSQTRTF (R) 10 m A/F|N/S/Q|T/R|T\F 80.0 8 4 7 3 0 unbiased m:Oxidized methionine 34.861668 0.903651 609.7801 1202.562 15.9905 -3.7 0.8999999999999995 9.5 3042.6 smORF_v4|nuORFdb_human LOC101928979_1:145382210-145382290:+ LOC101928979_1:145382210-145382290:+|ENST00000611275.4_2_1:145382212-145382344:-|T020086_1:149224691-149224769:+ LOC101928979 lncRNA 1:145382210-145382290:+ 1 LINC01719 lincRNA lncRNA GN=LOC101928979 E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep3_Slot2-5_1_3521 SCP E_20221201_NO30_400nL_HLAc1_4e7_PDAC_Slot2-6_1_3524.11992.1428.2 2 3 1 19.67 8.77 13.68 10.9 94.6 8 1 1 81900.0 Hydrogen 184 (D) 9 MAIEAQQKF (K) 9 MALEFnRLA M/A/I|E|A/Q|Q|K\F 100.0 9 5 7 4 0 dual b/y bias 41.428574 0.858854 533.272 1065.54 -0.003 -2.8 -0.3999999999999999 5.75 31124.2 nuORFdb_human ENST00000457761.6_1_2:172821936-172844354:+ ENST00000457761.6_1_2:172821936-172844354:+ HAT1 3' Overlap dORF 3' Overlap dORF GN=HAT1 3' Overlap dORF E_20221201_NO30_400nL_HLAc1_4e7_PDAC_Slot2-6_1_3524 Exploris_FAIMS VH20221207_MKP_FAIMS2022E_HLAc1_4e7_PDAC.31812.31812.2 2 1 1 8.25 2.42 8.47 5.83 62.2 8 1 1 54300000.0 Hydrogen 184 (D) 9 MAIEAQQKF (K) 9 MALEFnRLA M/A|I|E/A-Q/Q/K-F 100.0 9 2 6 2 2 y-ion bias 0.979 93.7 61.57 6.29 -50.0 533.2703 1065.54 -0.0065 -6.1 -3.4999999999999996 5.75 31124.2 nuORFdb_human ENST00000457761.6_1_2:172821936-172844354:+ ENST00000457761.6_1_2:172821936-172844354:+ HAT1 3' Overlap dORF 3' Overlap dORF GN=HAT1 3' Overlap dORF VH20221207_MKP_FAIMS2022E_HLAc1_4e7_PDAC Eva-50mio-3fr AL20210827_EV_HLA_PDAC_PANFRO413_2D_50e6_W632_classI_fxn1.14937.15037.2 2 1 1 12.71 6.13 7.89 6.57 78.3 7 1 1 12500000.0 M184m Hydrogen 184 (D) 9 mAIEAQQKF (K) 9 mALEFnRLA m/A|I|E/A Q/Q/K-F 77.8 7 2 6 2 1 y-ion bias m:Oxidized methionine 0.974 100.0 29.58 100.0 0.0 541.2709 1065.54 15.9947 -0.2 -0.30000000000000004 5.75 31124.2 nuORFdb_human ENST00000457761.6_1_2:172821936-172844354:+ ENST00000457761.6_1_2:172821936-172844354:+ HAT1 3' Overlap dORF 3' Overlap dORF GN=HAT1 3' Overlap dORF AL20210827_EV_HLA_PDAC_PANFRO413_2D_50e6_W632_classI_fxn1 SCP E_20221201_NO30_400nL_HLAc1_4e7_PDAC_Slot2-6_1_3524.8645.1427.2 2 3 1 17.36 7.84 9.46 9.51 87.9 8 1 1 15500.0 M184m Hydrogen 184 (D) 9 mAIEAQQKF (K) 9 mALEFnRLA m/A|I|E|A/Q|Q|K\F 100.0 9 6 7 5 0 dual b/y bias m:Oxidized methionine 29.866337 0.859447 541.2695 1065.54 15.993 -1.8 0.5999999999999999 5.75 31124.2 nuORFdb_human ENST00000457761.6_1_2:172821936-172844354:+ ENST00000457761.6_1_2:172821936-172844354:+ HAT1 3' Overlap dORF 3' Overlap dORF GN=HAT1 3' Overlap dORF E_20221201_NO30_400nL_HLAc1_4e7_PDAC_Slot2-6_1_3524 Exploris_FAIMS VH20221207_MKP_FAIMS2022E_HLAc1_4e7_PDAC.23722.23722.2 2 2 1 12.4 6.6 7.67 5.8 76.5 7 1 1 34300000.0 M184m Hydrogen 184 (D) 9 mAIEAQQKF (K) 9 mALEFnRLA m/A|I|E/A Q-Q/K-F 77.8 7 2 5 2 2 y-ion bias m:Oxidized methionine 0.966 87.7 49.15 47.78 -50.0 541.2699 1065.54 15.9928 -2.0 0.6000000000000001 5.75 31124.2 nuORFdb_human ENST00000457761.6_1_2:172821936-172844354:+ ENST00000457761.6_1_2:172821936-172844354:+ HAT1 3' Overlap dORF 3' Overlap dORF GN=HAT1 3' Overlap dORF VH20221207_MKP_FAIMS2022E_HLAc1_4e7_PDAC 40mio-rep1 E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep1_Slot1-04_1_3496.14070.1477.2 2 1 1 7.53 6.48 0.51 1.05 76.6 5 1 1 8270.0 Hydrogen 2 (K) 9 MAIPGRQPL (-) 9 MALFKYPL M/A/I|P/G R Q\P L 44.4 4 2 4 1 0 y-ion bias 48.602303 0.827899 491.7774 982.55 -0.0035 -3.6 -0.3999999999999999 9.5 1110.4 nuORFdb_human ENST00000540900.7_1_17:28443858-28499569:+ ENST00000540900.7_1_17:28443858-28499569:+ NSRP1 ncRNA Processed Transcript lncRNA GN=NSRP1 ncRNA Processed Transcript E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep1_Slot1-04_1_3496 40mio-rep3 E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep3_Slot2-5_1_3521.13761.1483.2 2 1 1 7.5 5.94 1.56 1.56 77.9 6 1 1 5540.0 Hydrogen 2 (K) 9 MAIPGRQPL (-) 9 MAIPSKPPAA M/A/I|P/G R-Q\P L 55.6 5 2 4 1 1 y-ion bias 47.54047 0.824096 491.7748 982.55 -0.0075 -7.7 -3.1000000000000005 9.5 1110.4 nuORFdb_human ENST00000540900.7_1_17:28443858-28499569:+ ENST00000540900.7_1_17:28443858-28499569:+ NSRP1 ncRNA Processed Transcript lncRNA GN=NSRP1 ncRNA Processed Transcript E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep3_Slot2-5_1_3521 40mio-rep4 E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep4_Slot2-5_1_3522.13784.1484.2 2 1 1 8.68 7.61 2.04 1.06 84.6 6 1 1 5380.0 Hydrogen 2 (K) 9 MAIPGRQPL (-) 9 MALLHLWV M/A/I|P/G R\Q\P L 55.6 5 3 4 1 0 unbiased 47.603226 0.823499 491.7765 982.55 -0.0035 -3.6 1.1 9.5 1110.4 nuORFdb_human ENST00000540900.7_1_17:28443858-28499569:+ ENST00000540900.7_1_17:28443858-28499569:+ NSRP1 ncRNA Processed Transcript lncRNA GN=NSRP1 ncRNA Processed Transcript E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep4_Slot2-5_1_3522 40mio-rep3 E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep3_Slot2-5_1_3521.9830.1461.2 2 1 1 8.04 6.47 1.81 1.57 51.8 6 1 1 6610.0 M2m Hydrogen 2 (K) 9 mAIPGRQPL (-) 9 mALFKYPL m/A/I|P/G R Q\P\L 66.7 6 3 4 1 0 unbiased m:Oxidized methionine 33.959942 0.83778 499.7738 982.55 15.9905 -4.4 0.1999999999999993 9.5 1110.4 nuORFdb_human ENST00000540900.7_1_17:28443858-28499569:+ ENST00000540900.7_1_17:28443858-28499569:+ NSRP1 ncRNA Processed Transcript lncRNA GN=NSRP1 ncRNA Processed Transcript E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep3_Slot2-5_1_3521 SCP E_20221201_NO30_400nL_HLAc1_4e7_PDAC_Slot2-6_1_3524.19344.552.1 1 1 1 9.93 1.88 7.72 8.06 64.7 6 0 0 12700.0 Hydrogen 24 (I) 9 MALVASPSM (G) 9 MTTSAWPL M A/L|V|A|S|P S\M 55.6 5 5 5 4 0 dual b/y bias 66.826553 1.394919 906.4386 906.442 -0.0033 -3.7 -1.3000000000000003 5.27 4633.5 nuORFdb_human ENST00000415933.5_1_7:44839032-44839382:+ ENST00000415933.5_1_7:44839032-44839382:+ PPIA Out-of-Frame Out-of-Frame GN=PPIA Out-of-Frame E_20221201_NO30_400nL_HLAc1_4e7_PDAC_Slot2-6_1_3524 SCP E_20221201_NO30_400nL_HLAc1_4e7_PDAC_Slot2-6_1_3524.14038.681.1 1 2 1 8.47 2.62 4.05 5.85 70.8 6 0 0 37700.0 Hydrogen 1 (-) 9 MAMAAVAAA (A) 9 ALGLCMAA M A M\A\A\V\A\A\A 66.7 6 6 0 0 0 b-ion bias 48.496719 1.317793 806.3882 806.39 -0.0019 -2.4 0.0 5.27 2088.4 smORF_v4|nuORFdb_human ATG4D_19:10654596-10654666:+ ATG4D_19:10654596-10654666:+|ENST00000586417.5_1_19:10654595-10654664:+ ATG4D uORF 19:10654596-10654666:+ 19 ATG4D 5' uORF 5' uORF GN=ATG4D E_20221201_NO30_400nL_HLAc1_4e7_PDAC_Slot2-6_1_3524 SCP E_20221201_NO30_400nL_HLAc1_4e7_PDAC_Slot2-6_1_3524.14021.443.1 1 1 1 9.37 4.43 3.96 4.94 73.9 7 0 0 3460.0 Hydrogen 46 (A) 9 MATTSSPCW (R) 9 LcSRPCW M A/T/T/S/S/P|C\W 77.8 7 2 6 1 0 y-ion bias Carbamidomethylation C:Carbamidomethylation, 48.438004 1.459645 1040.4137 1040.418 -0.0076 -7.3 -4.9 5.27 9762.0 nuORFdb_human ENST00000356346.7_1_8:145011235-145047603:- ENST00000356346.7_1_8:145011235-145047603:- PLEC Out-of-Frame Out-of-Frame GN=PLEC Out-of-Frame E_20221201_NO30_400nL_HLAc1_4e7_PDAC_Slot2-6_1_3524 40mio-rep1 E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep1_Slot1-04_1_3496.18193.1461.2 2 1 1 11.07 7.81 4.88 3.26 72.0 7 1 1 6860.0 Hydrogen 49 (A) 9 MEAPGLGRW (R) 9 EMAPGRGLW M/E|A|P/G/L|G R-W 77.8 7 3 6 3 1 dual b/y bias 62.845299 0.838283 508.7507 1016.498 -0.0035 -3.4 -0.19999999999999973 5.75 9324.5 nuORFdb_human ENST00000393623.6_2_19:10610689-10613572:- ENST00000393623.6_2_19:10610689-10613572:- KEAP1 5' Overlap uORF 5' Overlap uORF GN=KEAP1 5' Overlap uORF E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep1_Slot1-04_1_3496 40mio-rep1 E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep1_Slot1-04_1_3496.14756.1458.2 2 2 1 12.16 10.06 2.62 2.1 78.4 5 1 1 25100.0 M49m Hydrogen 49 (A) 9 mEAPGLGRW (R) 9 EmAPGRGLW m/E|A|P/G L|G R W 44.4 4 3 5 3 0 dual b/y bias m:Oxidized methionine 50.971397 0.839922 516.7483 1016.498 15.9905 -4.3 -1.0999999999999996 5.75 9324.5 nuORFdb_human ENST00000393623.6_2_19:10610689-10613572:- ENST00000393623.6_2_19:10610689-10613572:- KEAP1 5' Overlap uORF 5' Overlap uORF GN=KEAP1 5' Overlap uORF E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep1_Slot1-04_1_3496 40mio-rep2 E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep2_Slot2-5_1_3520.14446.1470.2 2 1 1 10.78 8.67 5.31 2.11 77.2 5 1 1 11100.0 M49m Hydrogen 49 (A) 9 mEAPGLGRW (R) 9 EmAPGRGLW m E|A|P/G L|G R\W 33.3 3 4 4 3 0 dual b/y bias m:Oxidized methionine 49.902168 0.832232 516.7478 1016.498 15.9905 -4.3 0.6000000000000005 5.75 9324.5 nuORFdb_human ENST00000393623.6_2_19:10610689-10613572:- ENST00000393623.6_2_19:10610689-10613572:- KEAP1 5' Overlap uORF 5' Overlap uORF GN=KEAP1 5' Overlap uORF E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep2_Slot2-5_1_3520 40mio-rep3 E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep3_Slot2-5_1_3521.14446.1476.2 2 2 1 13.78 10.62 5.27 3.16 83.3 6 1 1 22000.0 M49m Hydrogen 49 (A) 9 mEAPGLGRW (R) 9 EmAPGRGLW m/E|A|P/G L|G R\W 55.6 5 4 5 3 0 dual b/y bias m:Oxidized methionine 49.905254 0.828572 516.7499 1016.498 15.9945 -0.4 4.199999999999999 5.75 9324.5 nuORFdb_human ENST00000393623.6_2_19:10610689-10613572:- ENST00000393623.6_2_19:10610689-10613572:- KEAP1 5' Overlap uORF 5' Overlap uORF GN=KEAP1 5' Overlap uORF E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep3_Slot2-5_1_3521 40mio-rep4 E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep4_Slot2-5_1_3522.14448.1476.2 2 2 1 12.11 9.44 4.74 2.67 71.7 5 1 1 19800.0 M49m Hydrogen 49 (A) 9 mEAPGLGRW (R) 9 EmAPGRGLW m E|A|P\G\L|G R W 44.4 4 5 3 3 0 dual b/y bias m:Oxidized methionine 49.898167 0.82841 516.7471 1016.498 15.9885 -6.2 -1.5 5.75 9324.5 nuORFdb_human ENST00000393623.6_2_19:10610689-10613572:- ENST00000393623.6_2_19:10610689-10613572:- KEAP1 5' Overlap uORF 5' Overlap uORF GN=KEAP1 5' Overlap uORF E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep4_Slot2-5_1_3522 40mio-rep1 E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep1_Slot1-04_1_3496.21435.1271.2 2 1 1 12.88 6.88 6.46 6.0 82.7 7 0 0 5980.0 Hydrogen 40 (L) 10 MEEPISLHTW (F) 10 mAEPQAERPR M E|E|P/I/S L/H-T\W 60.0 6 3 5 2 1 unbiased 74.04776 0.957001 621.793 1242.582 -0.0036 -2.9 0.30000000000000027 4.51 6961.0 nuORFdb_human ENST00000318410.11_1_8:126096196-126103965:- ENST00000318410.11_1_8:126096196-126103965:- WASHC5 5' uORF 5' uORF GN=WASHC5 5' uORF E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep1_Slot1-04_1_3496 10mio-rep2 E_20221201_NO30_400nL_HLAc1_1e7_directIP_titration_rep2_Slot2-3_1_3513.19119.1285.2 2 1 1 9.16 4.33 4.25 4.83 63.1 5 0 0 3090.0 M40m Hydrogen 40 (L) 10 mEEPISLHTW (F) 10 mEEITRFWM m E|E|P I/S L/H/T W 20.0 2 2 5 2 0 y-ion bias m:Oxidized methionine 66.040657 0.947987 629.787 1242.582 15.9844 -8.4 -1.7000000000000002 4.51 6961.0 nuORFdb_human ENST00000318410.11_1_8:126096196-126103965:- ENST00000318410.11_1_8:126096196-126103965:- WASHC5 5' uORF 5' uORF GN=WASHC5 5' uORF E_20221201_NO30_400nL_HLAc1_1e7_directIP_titration_rep2_Slot2-3_1_3513 20mio-rep1 E_20221201_NO30_400nL_HLAc1_2e7_directIP_titration_rep1_Slot2-4_1_3515.19069.1280.2 2 3 1 16.54 4.76 10.93 11.78 75.2 7 0 0 11100.0 M40m Hydrogen 40 (L) 10 mEEPISLHTW (F) 10 EEmGFLRDVF m E/E|P/I|S L|H|T\W 60.0 6 5 6 4 0 dual b/y bias m:Oxidized methionine 65.854881 0.951353 629.7875 1242.582 15.9844 -8.4 -2.2 4.51 6961.0 nuORFdb_human ENST00000318410.11_1_8:126096196-126103965:- ENST00000318410.11_1_8:126096196-126103965:- WASHC5 5' uORF 5' uORF GN=WASHC5 5' uORF E_20221201_NO30_400nL_HLAc1_2e7_directIP_titration_rep1_Slot2-4_1_3515 20mio-rep2 E_20221201_NO30_400nL_HLAc1_2e7_directIP_titration_rep3_Slot2-4_1_3518.19129.1277.2 2 2 1 17.88 8.15 10.25 9.73 87.1 8 0 0 7150.0 M40m Hydrogen 40 (L) 10 mEEPISLHTW (F) 10 mEEIVRAMTY m/E|E|P/I|S|L/H\T\W 100.0 10 6 7 4 0 dual b/y bias m:Oxidized methionine 66.077797 0.953427 629.7873 1242.582 15.9844 -8.4 -2.1000000000000005 4.51 6961.0 nuORFdb_human ENST00000318410.11_1_8:126096196-126103965:- ENST00000318410.11_1_8:126096196-126103965:- WASHC5 5' uORF 5' uORF GN=WASHC5 5' uORF E_20221201_NO30_400nL_HLAc1_2e7_directIP_titration_rep3_Slot2-4_1_3518 20mio-rep3 E_20221201_NO30_400nL_HLAc1_2e7_directIP_titration_rep4_Slot2-4_1_3519.19128.1274.2 2 2 1 18.24 9.79 8.26 8.45 87.2 8 0 0 8820.0 M40m Hydrogen 40 (L) 10 mEEPISLHTW (F) 10 mEEIVRAMTY m/E|E|P\I|S/L/H|T\W 100.0 10 6 7 4 0 dual b/y bias m:Oxidized methionine 66.064537 0.954849 629.7881 1242.582 15.9864 -6.8 -1.0 4.51 6961.0 nuORFdb_human ENST00000318410.11_1_8:126096196-126103965:- ENST00000318410.11_1_8:126096196-126103965:- WASHC5 5' uORF 5' uORF GN=WASHC5 5' uORF E_20221201_NO30_400nL_HLAc1_2e7_directIP_titration_rep4_Slot2-4_1_3519 40mio-rep1 E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep1_Slot1-04_1_3496.19333.1251.2 2 2 1 15.09 5.88 9.21 9.21 80.5 8 0 0 13900.0 M40m Hydrogen 40 (L) 10 mEEPISLHTW (F) 10 mEEENRIPQP m E|E|P/I|S/L/H\T\W 80.0 8 5 6 3 0 unbiased m:Oxidized methionine 66.786087 0.969075 629.7904 1242.582 15.9904 -3.6 -0.3999999999999999 4.51 6961.0 nuORFdb_human ENST00000318410.11_1_8:126096196-126103965:- ENST00000318410.11_1_8:126096196-126103965:- WASHC5 5' uORF 5' uORF GN=WASHC5 5' uORF E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep1_Slot1-04_1_3496 40mio-rep2 E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep2_Slot2-5_1_3520.19040.1256.2 2 4 1 15.18 7.37 7.81 7.81 78.1 8 0 0 18800.0 M40m Hydrogen 40 (L) 10 mEEPISLHTW (F) 10 mEEENRIPQP m/E|E|P I/S/L/H|T\W 80.0 8 4 7 3 0 unbiased m:Oxidized methionine 65.774391 0.966018 629.7893 1242.582 15.9884 -5.2 -0.2999999999999998 4.51 6961.0 nuORFdb_human ENST00000318410.11_1_8:126096196-126103965:- ENST00000318410.11_1_8:126096196-126103965:- WASHC5 5' uORF 5' uORF GN=WASHC5 5' uORF E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep2_Slot2-5_1_3520 40mio-rep3 E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep3_Slot2-5_1_3521.19095.1267.2 2 6 1 17.07 7.53 8.99 9.54 85.4 7 0 0 25800.0 M40m Hydrogen 40 (L) 10 mEEPISLHTW (F) 10 mEEFFISPKD m E|E|P I|S/L/H|T\W 60.0 6 5 6 4 0 dual b/y bias m:Oxidized methionine 65.961823 0.959678 629.791 1242.582 15.9924 -2.0 2.5999999999999996 4.51 6961.0 nuORFdb_human ENST00000318410.11_1_8:126096196-126103965:- ENST00000318410.11_1_8:126096196-126103965:- WASHC5 5' uORF 5' uORF GN=WASHC5 5' uORF E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep3_Slot2-5_1_3521 40mio-rep4 E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep4_Slot2-5_1_3522.19064.1257.2 2 4 1 12.91 7.26 5.65 5.65 77.5 7 0 0 15400.0 M40m Hydrogen 40 (L) 10 mEEPISLHTW (F) 10 mEEVQDELIH m E\E|P I|S\L/H\T\W 60.0 6 6 3 2 0 b-ion bias m:Oxidized methionine 65.83757 0.96577 629.7883 1242.582 15.9864 -6.8 -2.0999999999999996 4.51 6961.0 nuORFdb_human ENST00000318410.11_1_8:126096196-126103965:- ENST00000318410.11_1_8:126096196-126103965:- WASHC5 5' uORF 5' uORF GN=WASHC5 5' uORF E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep4_Slot2-5_1_3522 40mio-rep1 E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep1_Slot1-04_1_3496.26269.1368.2 2 2 1 9.3 6.63 3.31 2.67 65.4 5 0 0 7130.0 M1m Hydrogen 1 (-) 9 mEEPTLQLF (C) 9 LQGSnTnAGIF m E E\P T\L\Q|L\F 44.4 4 5 1 1 0 b-ion bias m:Oxidized methionine 90.75029 0.896681 562.268 1107.539 15.9896 -4.7 -1.5 3.79 2171.5 smORF_v4|nuORFdb_human MAN1A2_1:117910471-117910528:+ MAN1A2_1:117910471-117910528:+|ENST00000356554.7_1_1:117910471-117910525:+ MAN1A2 uORF 1:117910471-117910528:+ 1 MAN1A2 5' uORF 5' uORF GN=MAN1A2 E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep1_Slot1-04_1_3496 40mio-rep2 E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep2_Slot2-5_1_3520.26013.1390.2 2 1 1 10.0 6.68 4.13 3.32 65.2 5 0 0 5120.0 M1m Hydrogen 1 (-) 9 mEEPTLQLF (C) 9 LQGSnTnAGIF m E E\P T\L\Q|L\F 44.4 4 5 1 1 0 b-ion bias m:Oxidized methionine 89.861984 0.882438 562.2675 1107.539 15.9876 -6.5 -1.5999999999999996 3.79 2171.5 smORF_v4|nuORFdb_human MAN1A2_1:117910471-117910528:+ MAN1A2_1:117910471-117910528:+|ENST00000356554.7_1_1:117910471-117910525:+ MAN1A2 uORF 1:117910471-117910528:+ 1 MAN1A2 5' uORF 5' uORF GN=MAN1A2 E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep2_Slot2-5_1_3520 40mio-rep3 E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep3_Slot2-5_1_3521.26013.1395.2 2 1 1 9.57 7.14 2.43 2.43 70.2 6 0 0 2780.0 M1m Hydrogen 1 (-) 9 mEEPTLQLF (C) 9 EGDSLNTQLF m E\E\P T\L\Q|L\F 55.6 5 6 1 1 0 b-ion bias m:Oxidized methionine 89.857567 0.879635 562.2675 1107.539 15.9896 -4.7 -0.10000000000000053 3.79 2171.5 smORF_v4|nuORFdb_human MAN1A2_1:117910471-117910528:+ MAN1A2_1:117910471-117910528:+|ENST00000356554.7_1_1:117910471-117910525:+ MAN1A2 uORF 1:117910471-117910528:+ 1 MAN1A2 5' uORF 5' uORF GN=MAN1A2 E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep3_Slot2-5_1_3521 40mio-rep4 E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep4_Slot2-5_1_3522.26026.1385.2 2 1 1 11.73 8.7 2.74 3.03 80.0 5 0 0 3180.0 M1m Hydrogen 1 (-) 9 mEEPTLQLF (C) 9 LQGSnTnAGIF m E E\P T\L\Q|L\F 44.4 4 5 1 1 0 b-ion bias m:Oxidized methionine 89.893318 0.885895 562.2685 1107.539 15.9916 -2.9 1.8000000000000003 3.79 2171.5 smORF_v4|nuORFdb_human MAN1A2_1:117910471-117910528:+ MAN1A2_1:117910471-117910528:+|ENST00000356554.7_1_1:117910471-117910525:+ MAN1A2 uORF 1:117910471-117910528:+ 1 MAN1A2 5' uORF 5' uORF GN=MAN1A2 E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep4_Slot2-5_1_3522 40mio-rep1 E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep1_Slot1-04_1_3496.26632.1310.2 2 1 1 9.3 9.3 9.3 64.7 8 1 1 3380.0 Hydrogen 68 (G) 10 MEFMVLAQKF (-) 10 M E|F|M|V\L/A|Q/K\F 80.0 8 6 6 4 0 dual b/y bias 92.004166 0.932401 622.3135 1243.621 -0.0026 -2.1 1.1 5.75 8645.7 nuORFdb_human ENST00000484616.2_1_6:133135907-133136327:+ ENST00000484616.2_1_6:133135907-133136327:+ RPS12 ncRNA Retained Intron lncRNA GN=RPS12 ncRNA Retained Intron E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep1_Slot1-04_1_3496 40mio-rep3 E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep3_Slot2-5_1_3521.12407.1622.3 3 1 1 8.43 4.07 2.25 4.36 77.7 5 1 1 4080.0 Hydrogen 50 (S) 9 MEFPGRSHF (K) 9 MEPAQKPCF M/E|F/P/G R S H-F 55.6 5 1 4 1 1 y-ion bias 42.864864 0.736619 369.8372 1107.504 -0.0076 -6.8 -2.2 6.5 7111.9 nuORFdb_human ENST00000367728.1_1_1:172411833-172412016:- ENST00000367728.1_1_1:172411833-172412016:-|ENST00000478184.1_1_1:172411833-172412978:- PIGC 5' uORF 5' uORF GN=PIGC 5' uORF E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep3_Slot2-5_1_3521 40mio-rep2 E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep2_Slot2-5_1_3520.23853.1337.2 2 1 1 12.67 8.37 5.25 4.3 79.2 6 1 1 5320.0 Hydrogen 4 (A) 10 MEGDKLILLY (-) 10 mAQALITILY M/E/G D K L\I\L|L\Y 60.0 6 4 3 1 0 unbiased 82.399971 0.915953 597.823 1194.644 -0.0051 -4.3 0.6000000000000005 4.37 1491.8 nuORFdb_human ENST00000318948.6_1_21:16340594-16340633:- ENST00000318948.6_1_21:16340594-16340633:- NRIP1 5' uORF 5' uORF GN=NRIP1 5' uORF E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep2_Slot2-5_1_3520 40mio-rep3 E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep3_Slot2-5_1_3521.23902.1337.2 2 1 1 8.13 5.78 3.58 2.35 66.2 6 1 1 3410.0 Hydrogen 4 (A) 10 MEGDKLILLY (-) 10 mAQALITILY M E/G D\K L-I\L|L\Y 40.0 4 4 2 1 1 b-ion bias 82.56749 0.915968 597.8222 1194.644 -0.0071 -6.0 -1.4000000000000004 4.37 1491.8 nuORFdb_human ENST00000318948.6_1_21:16340594-16340633:- ENST00000318948.6_1_21:16340594-16340633:- NRIP1 5' uORF 5' uORF GN=NRIP1 5' uORF E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep3_Slot2-5_1_3521 10mio-rep2 E_20221201_NO30_400nL_HLAc1_1e7_directIP_titration_rep2_Slot2-3_1_3513.22230.1339.2 2 1 1 11.26 9.61 1.97 1.65 84.5 5 1 1 2560.0 M4m Hydrogen 4 (A) 10 mEGDKLILLY (-) 10 ENISmVLLIY m E/G D K L\I\L|L\Y 40.0 4 4 2 1 0 b-ion bias m:Oxidized methionine 76.783287 0.91442 605.8177 1194.644 15.9849 -8.3 -1.6000000000000005 4.37 1491.8 nuORFdb_human ENST00000318948.6_1_21:16340594-16340633:- ENST00000318948.6_1_21:16340594-16340633:- NRIP1 5' uORF 5' uORF GN=NRIP1 5' uORF E_20221201_NO30_400nL_HLAc1_1e7_directIP_titration_rep2_Slot2-3_1_3513 10mio-rep3 E_20221201_NO30_400nL_HLAc1_1e7_directIP_titration_rep3_Slot2-3_1_3514.22188.1341.2 2 1 1 8.65 7.61 1.05 1.05 77.2 5 1 1 1910.0 M4m Hydrogen 4 (A) 10 mEGDKLILLY (-) 10 WNNFQLLIY m E\G D K L\I|L|L\Y 40.0 4 5 2 2 0 b-ion bias m:Oxidized methionine 76.643402 0.91295 605.8193 1194.644 15.9869 -6.7 -0.40000000000000036 4.37 1491.8 nuORFdb_human ENST00000318948.6_1_21:16340594-16340633:- ENST00000318948.6_1_21:16340594-16340633:- NRIP1 5' uORF 5' uORF GN=NRIP1 5' uORF E_20221201_NO30_400nL_HLAc1_1e7_directIP_titration_rep3_Slot2-3_1_3514 20mio-rep2 E_20221201_NO30_400nL_HLAc1_2e7_directIP_titration_rep3_Slot2-4_1_3518.22247.1335.2 2 2 1 12.12 9.97 2.15 2.15 80.3 5 1 1 7270.0 M4m Hydrogen 4 (A) 10 mEGDKLILLY (-) 10 ENISmVLLIY m E|G D K L\I|L|L\Y 40.0 4 5 3 3 0 unbiased m:Oxidized methionine 76.843086 0.917101 605.8191 1194.644 15.9869 -6.7 -0.40000000000000036 4.37 1491.8 nuORFdb_human ENST00000318948.6_1_21:16340594-16340633:- ENST00000318948.6_1_21:16340594-16340633:- NRIP1 5' uORF 5' uORF GN=NRIP1 5' uORF E_20221201_NO30_400nL_HLAc1_2e7_directIP_titration_rep3_Slot2-4_1_3518 20mio-rep3 E_20221201_NO30_400nL_HLAc1_2e7_directIP_titration_rep4_Slot2-4_1_3519.22276.1338.2 2 1 1 13.17 11.87 1.34 1.3 88.7 5 1 1 4310.0 M4m Hydrogen 4 (A) 10 mEGDKLILLY (-) 10 LIDmDKALLY m E/G D K L\I|L|L\Y 40.0 4 4 3 2 0 unbiased m:Oxidized methionine 76.934464 0.914828 605.8206 1194.644 15.9909 -3.4 2.4 4.37 1491.8 nuORFdb_human ENST00000318948.6_1_21:16340594-16340633:- ENST00000318948.6_1_21:16340594-16340633:- NRIP1 5' uORF 5' uORF GN=NRIP1 5' uORF E_20221201_NO30_400nL_HLAc1_2e7_directIP_titration_rep4_Slot2-4_1_3519 40mio-rep1 E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep1_Slot1-04_1_3496.22520.1309.2 2 2 1 13.53 10.8 2.73 2.73 86.5 6 1 1 21200.0 M4m Hydrogen 4 (A) 10 mEGDKLILLY (-) 10 ENISmVLLIY m E/G D\K L\I|L|L\Y 40.0 4 5 3 2 0 unbiased m:Oxidized methionine 77.797279 0.932951 605.8209 1194.644 15.9909 -3.4 -0.19999999999999973 4.37 1491.8 nuORFdb_human ENST00000318948.6_1_21:16340594-16340633:- ENST00000318948.6_1_21:16340594-16340633:- NRIP1 5' uORF 5' uORF GN=NRIP1 5' uORF E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep1_Slot1-04_1_3496 40mio-rep2 E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep2_Slot2-5_1_3520.22300.1325.2 2 1 1 14.14 11.45 2.69 2.69 83.5 6 1 1 11500.0 M4m Hydrogen 4 (A) 10 mEGDKLILLY (-) 10 ENISmVLLIY m E/G D\K L\I|L|L\Y 40.0 4 5 3 2 0 unbiased m:Oxidized methionine 77.03672 0.922957 605.8194 1194.644 15.9869 -6.7 -1.7999999999999998 4.37 1491.8 nuORFdb_human ENST00000318948.6_1_21:16340594-16340633:- ENST00000318948.6_1_21:16340594-16340633:- NRIP1 5' uORF 5' uORF GN=NRIP1 5' uORF E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep2_Slot2-5_1_3520 40mio-rep3 E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep3_Slot2-5_1_3521.22311.1331.2 2 1 1 11.86 10.15 1.71 1.71 76.7 5 1 1 10200.0 M4m Hydrogen 4 (A) 10 mEGDKLILLY (-) 10 ENISmVLLIY m E G D\K L\I|L|L\Y 40.0 4 5 2 2 0 b-ion bias m:Oxidized methionine 77.072769 0.919195 605.8196 1194.644 15.9889 -5.0 -0.40000000000000036 4.37 1491.8 nuORFdb_human ENST00000318948.6_1_21:16340594-16340633:- ENST00000318948.6_1_21:16340594-16340633:- NRIP1 5' uORF 5' uORF GN=NRIP1 5' uORF E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep3_Slot2-5_1_3521 40mio-rep4 E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep4_Slot2-5_1_3522.22268.1328.2 2 1 1 13.21 9.54 2.67 3.67 70.0 7 1 1 7280.0 M4m Hydrogen 4 (A) 10 mEGDKLILLY (-) 10 LIDmDKALLY m E/G D\K\L\I|L|L\Y 60.0 6 6 3 2 0 b-ion bias m:Oxidized methionine 76.909622 0.921206 605.8201 1194.644 15.9889 -5.0 -0.2999999999999998 4.37 1491.8 nuORFdb_human ENST00000318948.6_1_21:16340594-16340633:- ENST00000318948.6_1_21:16340594-16340633:- NRIP1 5' uORF 5' uORF GN=NRIP1 5' uORF E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep4_Slot2-5_1_3522 10mio-rep3 E_20221201_NO30_400nL_HLAc1_1e7_directIP_titration_rep3_Slot2-3_1_3514.17895.1402.2 2 1 1 7.44 7.44 7.44 60.7 8 0 0 4890.0 Hydrogen 8 (I) 10 MEINHFSSGF (-) 10 M E/I/N/H\F\S\S\G\F 80.0 8 5 3 0 0 unbiased 61.815929 0.874794 584.7534 1168.509 -0.0104 -8.9 -2.6000000000000005 5.22 1954.2 nuORFdb_human ENST00000301665.7_1_17:38975457-38989417:+ ENST00000301665.7_1_17:38975457-38989417:+ TMEM99 5' uORF 5' uORF GN=TMEM99 5' uORF E_20221201_NO30_400nL_HLAc1_1e7_directIP_titration_rep3_Slot2-3_1_3514 20mio-rep1 E_20221201_NO30_400nL_HLAc1_2e7_directIP_titration_rep1_Slot2-4_1_3515.17906.1388.2 2 1 1 13.13 5.18 9.03 7.95 72.8 9 0 0 7340.0 Hydrogen 8 (I) 10 MEINHFSSGF (-) 10 YNEAFFSSGF M/E/I|N/H|F|S|S\G\F 100.0 10 6 7 4 0 dual b/y bias 61.840393 0.884085 584.7545 1168.509 -0.0084 -7.2 -1.0 5.22 1954.2 nuORFdb_human ENST00000301665.7_1_17:38975457-38989417:+ ENST00000301665.7_1_17:38975457-38989417:+ TMEM99 5' uORF 5' uORF GN=TMEM99 5' uORF E_20221201_NO30_400nL_HLAc1_2e7_directIP_titration_rep1_Slot2-4_1_3515 20mio-rep3 E_20221201_NO30_400nL_HLAc1_2e7_directIP_titration_rep4_Slot2-4_1_3519.17946.1383.2 2 1 1 8.91 3.53 7.28 5.38 66.4 7 0 0 9120.0 Hydrogen 8 (I) 10 MEINHFSSGF (-) 10 YNEAFFSSGF M E|I/N H\F\S|S|G\F 60.0 6 6 4 3 0 unbiased 61.983341 0.887243 584.7547 1168.509 -0.0064 -5.5 0.2999999999999998 5.22 1954.2 nuORFdb_human ENST00000301665.7_1_17:38975457-38989417:+ ENST00000301665.7_1_17:38975457-38989417:+ TMEM99 5' uORF 5' uORF GN=TMEM99 5' uORF E_20221201_NO30_400nL_HLAc1_2e7_directIP_titration_rep4_Slot2-4_1_3519 40mio-rep1 E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep1_Slot1-04_1_3496.18131.1354.2 2 3 1 18.13 6.01 10.51 12.13 87.9 9 0 0 28400.0 Hydrogen 8 (I) 10 MEINHFSSGF (-) 10 SVDHYWASGF M/E|I|N/H\F\S|S|G\F 100.0 10 7 6 4 0 dual b/y bias 62.631226 0.905168 584.7574 1168.509 -0.0024 -2.1 1.1 5.22 1954.2 nuORFdb_human ENST00000301665.7_1_17:38975457-38989417:+ ENST00000301665.7_1_17:38975457-38989417:+ TMEM99 5' uORF 5' uORF GN=TMEM99 5' uORF E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep1_Slot1-04_1_3496 40mio-rep2 E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep2_Slot2-5_1_3520.17840.1367.2 2 2 1 15.42 5.72 8.09 9.69 84.4 7 0 0 13300.0 Hydrogen 8 (I) 10 MEINHFSSGF (-) 10 SVDHYWASGF M E|I/N/H F\S|S\G\F 60.0 6 5 4 2 0 unbiased 61.62751 0.897178 584.7572 1168.509 -0.0024 -2.1 2.8000000000000003 5.22 1954.2 nuORFdb_human ENST00000301665.7_1_17:38975457-38989417:+ ENST00000301665.7_1_17:38975457-38989417:+ TMEM99 5' uORF 5' uORF GN=TMEM99 5' uORF E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep2_Slot2-5_1_3520 40mio-rep3 E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep3_Slot2-5_1_3521.17870.1374.2 2 1 1 15.36 3.31 11.4 12.05 79.2 9 0 0 7350.0 Hydrogen 8 (I) 10 MEINHFSSGF (-) 10 CQCLLPSSGF M/E|I/N/H/F|S|S\G\F 100.0 10 5 7 3 0 unbiased 61.73167 0.892367 584.7568 1168.509 -0.0024 -2.1 2.4999999999999996 5.22 1954.2 nuORFdb_human ENST00000301665.7_1_17:38975457-38989417:+ ENST00000301665.7_1_17:38975457-38989417:+ TMEM99 5' uORF 5' uORF GN=TMEM99 5' uORF E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep3_Slot2-5_1_3521 40mio-rep4 E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep4_Slot2-5_1_3522.17858.1375.2 2 1 1 13.98 4.87 8.21 9.11 76.2 9 0 0 9510.0 Hydrogen 8 (I) 10 MEINHFSSGF (-) 10 SVDHYWASGF M/E/I/N|H\F\S|S|G\F 100.0 10 6 6 3 0 unbiased 61.672112 0.892006 584.7556 1168.509 -0.0044 -3.8 0.9000000000000004 5.22 1954.2 nuORFdb_human ENST00000301665.7_1_17:38975457-38989417:+ ENST00000301665.7_1_17:38975457-38989417:+ TMEM99 5' uORF 5' uORF GN=TMEM99 5' uORF E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep4_Slot2-5_1_3522 10mio-rep1 E_20221201_NO30_400nL_HLAc1_1e7_directIP_titration_rep1_Slot2-3_1_3512.14218.1394.2 2 1 1 7.74 -0.03 6.75 7.77 54.9 6 0 0 7360.0 M8m Hydrogen 8 (I) 10 mEINHFSSGF (-) 10 SSSQVDQETGF m/E/I/N H F S|S\G\F 60.0 6 3 4 1 0 unbiased m:Oxidized methionine 49.117664 0.880168 592.7505 1168.509 15.9836 -9.6 -1.8999999999999995 5.22 1954.2 nuORFdb_human ENST00000301665.7_1_17:38975457-38989417:+ ENST00000301665.7_1_17:38975457-38989417:+ TMEM99 5' uORF 5' uORF GN=TMEM99 5' uORF E_20221201_NO30_400nL_HLAc1_1e7_directIP_titration_rep1_Slot2-3_1_3512 20mio-rep1 E_20221201_NO30_400nL_HLAc1_2e7_directIP_titration_rep1_Slot2-4_1_3515.14117.1389.2 2 1 1 8.64 8.64 8.64 53.2 6 0 0 12300.0 M8m Hydrogen 8 (I) 10 mEINHFSSGF (-) 10 m E/I|N H F|S\S|G\F 50.0 5 5 4 3 0 unbiased m:Oxidized methionine 48.757961 0.883467 592.753 1168.509 15.9896 -4.5 1.7000000000000002 5.22 1954.2 nuORFdb_human ENST00000301665.7_1_17:38975457-38989417:+ ENST00000301665.7_1_17:38975457-38989417:+ TMEM99 5' uORF 5' uORF GN=TMEM99 5' uORF E_20221201_NO30_400nL_HLAc1_2e7_directIP_titration_rep1_Slot2-4_1_3515 40mio-rep1 E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep1_Slot1-04_1_3496.14412.1343.2 2 1 1 13.0 5.42 7.58 7.58 69.2 7 0 0 20700.0 M8m Hydrogen 8 (I) 10 mEINHFSSGF (-) 10 cIPQNLSSGF m E|I|N/H F\S|S\G\F 60.0 6 6 4 3 0 unbiased m:Oxidized methionine 49.783726 0.912021 592.7546 1168.509 15.9936 -1.1 2.1 5.22 1954.2 nuORFdb_human ENST00000301665.7_1_17:38975457-38989417:+ ENST00000301665.7_1_17:38975457-38989417:+ TMEM99 5' uORF 5' uORF GN=TMEM99 5' uORF E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep1_Slot1-04_1_3496 40mio-rep2 E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep2_Slot2-5_1_3520.14075.1375.2 2 1 1 14.86 -0.39 9.34 15.25 76.1 8 0 0 11200.0 M8m Hydrogen 8 (I) 10 mEINHFSSGF (-) 10 GLPImESnCF m/E/I|N H|F\S|S\G\F 80.0 8 6 5 3 0 unbiased m:Oxidized methionine 48.621292 0.891841 592.7542 1168.509 15.9916 -2.8 2.1000000000000005 5.22 1954.2 nuORFdb_human ENST00000301665.7_1_17:38975457-38989417:+ ENST00000301665.7_1_17:38975457-38989417:+ TMEM99 5' uORF 5' uORF GN=TMEM99 5' uORF E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep2_Slot2-5_1_3520 40mio-rep3 E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep3_Slot2-5_1_3521.14106.1372.2 2 1 1 12.66 7.44 12.66 68.3 8 0 0 18900.0 M8m Hydrogen 8 (I) 10 mEINHFSSGF (-) 10 m/E|I|N H|F\S|S\G\F 80.0 8 7 5 4 0 dual b/y bias m:Oxidized methionine 48.731258 0.894154 592.7531 1168.509 15.9896 -4.5 0.09999999999999964 5.22 1954.2 nuORFdb_human ENST00000301665.7_1_17:38975457-38989417:+ ENST00000301665.7_1_17:38975457-38989417:+ TMEM99 5' uORF 5' uORF GN=TMEM99 5' uORF E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep3_Slot2-5_1_3521 40mio-rep4 E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep4_Slot2-5_1_3522.14076.1378.2 2 2 1 13.39 6.09 7.72 7.3 70.6 7 0 0 21300.0 M8m Hydrogen 8 (I) 10 mEINHFSSGF (-) 10 LLAAcmHSGF m E I|N/H|F\S|S\G\F 70.0 7 6 4 3 0 unbiased m:Oxidized methionine 48.614037 0.88982 592.7525 1168.509 15.9876 -6.2 -1.5 5.22 1954.2 nuORFdb_human ENST00000301665.7_1_17:38975457-38989417:+ ENST00000301665.7_1_17:38975457-38989417:+ TMEM99 5' uORF 5' uORF GN=TMEM99 5' uORF E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep4_Slot2-5_1_3522 40mio-rep1 E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep1_Slot1-04_1_3496.22455.1241.2 2 2 1 13.77 2.59 13.77 11.18 73.9 9 1 1 11600.0 Hydrogen 1 (-) 11 MELSDVLVKQY (S) 11 qQVVVLVRDNGD M E|L|S/D|V|L|V|K/Q\Y 81.8 9 7 8 6 0 dual b/y bias 77.572845 0.975727 662.8424 1324.682 -0.005 -3.8 -0.5999999999999996 4.37 6171.9 nuORFdb_human ENST00000380359.3_1_10:5493865-5494450:+ ENST00000380359.3_1_10:5493865-5494450:+ NET1 Out-of-Frame Out-of-Frame GN=NET1 Out-of-Frame E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep1_Slot1-04_1_3496 40mio-rep4 E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep4_Slot2-5_1_3522.22243.1267.2 2 1 1 9.54 9.54 9.54 57.7 7 1 1 3400.0 Hydrogen 1 (-) 11 MELSDVLVKQY (S) 11 M E|L|S D V|L|V|K/Q\Y 54.5 6 6 6 5 0 dual b/y bias 76.823334 0.959572 662.8427 1324.682 -0.003 -2.3 2.4000000000000004 4.37 6171.9 nuORFdb_human ENST00000380359.3_1_10:5493865-5494450:+ ENST00000380359.3_1_10:5493865-5494450:+ NET1 Out-of-Frame Out-of-Frame GN=NET1 Out-of-Frame E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep4_Slot2-5_1_3522 40mio-rep1 E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep1_Slot1-04_1_3496.19161.1221.2 2 2 1 9.87 9.87 9.87 59.5 8 1 1 8850.0 M1m Hydrogen 1 (-) 11 mELSDVLVKQY (S) 11 m E|L\S/D|V|L\V|K Q\Y 63.6 7 7 5 4 0 dual b/y bias m:Oxidized methionine 66.192192 0.987806 670.8401 1324.682 15.991 -2.9 0.30000000000000027 4.37 6171.9 nuORFdb_human ENST00000380359.3_1_10:5493865-5494450:+ ENST00000380359.3_1_10:5493865-5494450:+ NET1 Out-of-Frame Out-of-Frame GN=NET1 Out-of-Frame E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep1_Slot1-04_1_3496 40mio-rep2 E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep2_Slot2-5_1_3520.18816.1234.2 2 1 1 14.5 6.07 9.26 8.43 78.1 9 1 1 6250.0 M1m Hydrogen 1 (-) 11 mELSDVLVKQY (S) 11 IEDTLMTVKQY m E\L|S\D\V|L|V|K|Q\Y 81.8 9 9 5 5 0 dual b/y bias m:Oxidized methionine 65.001022 0.979981 670.8396 1324.682 15.991 -2.9 2.0000000000000004 4.37 6171.9 nuORFdb_human ENST00000380359.3_1_10:5493865-5494450:+ ENST00000380359.3_1_10:5493865-5494450:+ NET1 Out-of-Frame Out-of-Frame GN=NET1 Out-of-Frame E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep2_Slot2-5_1_3520 40mio-rep3 E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep3_Slot2-5_1_3521.18855.1237.2 2 1 1 16.75 6.06 11.17 10.69 80.6 9 1 1 3270.0 M1m Hydrogen 1 (-) 11 mELSDVLVKQY (S) 11 IEDTLMTVKQY m E\L|S\D\V|L|V|K|Q\Y 81.8 9 9 5 5 0 dual b/y bias m:Oxidized methionine 65.133018 0.97822 670.8373 1324.682 15.985 -7.4 -2.8000000000000007 4.37 6171.9 nuORFdb_human ENST00000380359.3_1_10:5493865-5494450:+ ENST00000380359.3_1_10:5493865-5494450:+ NET1 Out-of-Frame Out-of-Frame GN=NET1 Out-of-Frame E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep3_Slot2-5_1_3521 40mio-rep4 E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep4_Slot2-5_1_3522.18828.1233.2 2 1 1 16.4 2.33 13.31 14.07 75.3 9 1 1 3800.0 M1m Hydrogen 1 (-) 11 mELSDVLVKQY (S) 11 IEDTLMTVKQY m E/L|S|D|V|L|V/K/Q\Y 81.8 9 6 8 5 0 dual b/y bias m:Oxidized methionine 65.02285 0.980234 670.8393 1324.682 15.989 -4.4 0.2999999999999998 4.37 6171.9 nuORFdb_human ENST00000380359.3_1_10:5493865-5494450:+ ENST00000380359.3_1_10:5493865-5494450:+ NET1 Out-of-Frame Out-of-Frame GN=NET1 Out-of-Frame E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep4_Slot2-5_1_3522 40mio-rep1 E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep1_Slot1-04_1_3496.12904.1456.2 2 1 1 15.93 10.33 10.75 5.6 90.2 7 1 1 7810.0 Hydrogen 20 (A) 9 MELVTGKEY (S) 9 MELVTINYS M/E|L|V|T|G K\E\Y 77.8 7 6 5 4 0 dual b/y bias 44.574516 0.841264 535.2635 1069.523 -0.0047 -4.4 -1.2000000000000002 4.53 3659.0 nuORFdb_human ENST00000615206.4_1_12:56610319-56615392:- ENST00000615206.4_1_12:56610319-56615392:-|ENST00000345093.8_1_12:56610319-56615573:-|ENST00000552656.5_1_12:56610319-56615704:- RNF41 5' uORF 5' uORF GN=RNF41 5' uORF E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep1_Slot1-04_1_3496 40mio-rep2 E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep2_Slot2-5_1_3520.12589.1473.2 2 1 1 11.25 8.6 7.23 2.65 81.1 6 1 1 10000.0 Hydrogen 20 (A) 9 MELVTGKEY (S) 9 MELVTINYS M/E|L|V|T|G K E\Y 66.7 6 5 5 4 0 dual b/y bias 43.488876 0.8307 535.2622 1069.523 -0.0067 -6.3 -1.3999999999999995 4.53 3659.0 nuORFdb_human ENST00000615206.4_1_12:56610319-56615392:- ENST00000615206.4_1_12:56610319-56615392:-|ENST00000345093.8_1_12:56610319-56615573:-|ENST00000552656.5_1_12:56610319-56615704:- RNF41 5' uORF 5' uORF GN=RNF41 5' uORF E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep2_Slot2-5_1_3520 40mio-rep3 E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep3_Slot2-5_1_3521.12607.1472.2 2 1 1 7.63 5.83 5.32 1.81 59.2 6 1 1 7920.0 Hydrogen 20 (A) 9 MELVTGKEY (S) 9 MELVTINYS M/E|L|V|T|G K E\Y 66.7 6 5 5 4 0 dual b/y bias 43.555183 0.831094 535.2615 1069.523 -0.0067 -6.3 -1.7000000000000002 4.53 3659.0 nuORFdb_human ENST00000615206.4_1_12:56610319-56615392:- ENST00000615206.4_1_12:56610319-56615392:-|ENST00000345093.8_1_12:56610319-56615573:-|ENST00000552656.5_1_12:56610319-56615704:- RNF41 5' uORF 5' uORF GN=RNF41 5' uORF E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep3_Slot2-5_1_3521 40mio-rep4 E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep4_Slot2-5_1_3522.12585.1478.2 2 2 1 15.99 11.17 9.43 4.83 90.1 8 1 1 15800.0 Hydrogen 20 (A) 9 MELVTGKEY (S) 9 MELVTINYS M/E|L|V|T|G\K\E\Y 100.0 9 7 5 4 0 dual b/y bias 43.460369 0.827481 535.2631 1069.523 -0.0047 -4.4 0.2999999999999998 4.53 3659.0 nuORFdb_human ENST00000615206.4_1_12:56610319-56615392:- ENST00000615206.4_1_12:56610319-56615392:-|ENST00000345093.8_1_12:56610319-56615573:-|ENST00000552656.5_1_12:56610319-56615704:- RNF41 5' uORF 5' uORF GN=RNF41 5' uORF E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep4_Slot2-5_1_3522 20mio-rep1 E_20221201_NO30_400nL_HLAc1_2e7_directIP_titration_rep1_Slot2-4_1_3515.8878.1464.2 2 2 1 10.15 7.3 4.57 2.85 69.3 5 1 1 12900.0 M20m Hydrogen 20 (A) 9 mELVTGKEY (S) 9 mELVTINYS m E|L|V|T/G K E\Y 44.4 4 4 4 3 0 dual b/y bias m:Oxidized methionine 30.668011 0.836048 543.2604 1069.523 15.9893 -5.2 1.0 4.53 3659.0 nuORFdb_human ENST00000615206.4_1_12:56610319-56615392:- ENST00000615206.4_1_12:56610319-56615392:-|ENST00000345093.8_1_12:56610319-56615573:-|ENST00000552656.5_1_12:56610319-56615704:- RNF41 5' uORF 5' uORF GN=RNF41 5' uORF E_20221201_NO30_400nL_HLAc1_2e7_directIP_titration_rep1_Slot2-4_1_3515 20mio-rep2 E_20221201_NO30_400nL_HLAc1_2e7_directIP_titration_rep3_Slot2-4_1_3518.9040.1461.2 2 1 1 8.21 3.83 6.8 4.38 55.9 6 1 1 5530.0 M20m Hydrogen 20 (A) 9 mELVTGKEY (S) 9 mELVTINYS m E/L|V/T|G K\E\Y 55.6 5 4 4 2 0 unbiased m:Oxidized methionine 31.229136 0.838 543.2588 1069.523 15.9873 -7.0 -0.7000000000000002 4.53 3659.0 nuORFdb_human ENST00000615206.4_1_12:56610319-56615392:- ENST00000615206.4_1_12:56610319-56615392:-|ENST00000345093.8_1_12:56610319-56615573:-|ENST00000552656.5_1_12:56610319-56615704:- RNF41 5' uORF 5' uORF GN=RNF41 5' uORF E_20221201_NO30_400nL_HLAc1_2e7_directIP_titration_rep3_Slot2-4_1_3518 20mio-rep3 E_20221201_NO30_400nL_HLAc1_2e7_directIP_titration_rep4_Slot2-4_1_3519.8916.1461.2 2 2 1 9.85 7.1 4.38 2.75 69.5 6 1 1 9150.0 M20m Hydrogen 20 (A) 9 mELVTGKEY (S) 9 mELVTINYS m E|L|V|T/G K\E-Y 55.6 5 4 4 3 1 dual b/y bias m:Oxidized methionine 30.802319 0.838047 543.2592 1069.523 15.9873 -7.0 -1.2000000000000002 4.53 3659.0 nuORFdb_human ENST00000615206.4_1_12:56610319-56615392:- ENST00000615206.4_1_12:56610319-56615392:-|ENST00000345093.8_1_12:56610319-56615573:-|ENST00000552656.5_1_12:56610319-56615704:- RNF41 5' uORF 5' uORF GN=RNF41 5' uORF E_20221201_NO30_400nL_HLAc1_2e7_directIP_titration_rep4_Slot2-4_1_3519 40mio-rep2 E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep2_Slot2-5_1_3520.8888.1451.2 2 3 1 13.51 9.17 7.81 4.35 75.2 6 1 1 22100.0 M20m Hydrogen 20 (A) 9 mELVTGKEY (S) 9 mELVTINYS m E|L|V|T|G K\E\Y 55.6 5 6 4 4 0 dual b/y bias m:Oxidized methionine 30.705902 0.844633 543.2599 1069.523 15.9893 -5.2 -0.2999999999999998 4.53 3659.0 nuORFdb_human ENST00000615206.4_1_12:56610319-56615392:- ENST00000615206.4_1_12:56610319-56615392:-|ENST00000345093.8_1_12:56610319-56615573:-|ENST00000552656.5_1_12:56610319-56615704:- RNF41 5' uORF 5' uORF GN=RNF41 5' uORF E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep2_Slot2-5_1_3520 40mio-rep3 E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep3_Slot2-5_1_3521.8894.1448.2 2 2 1 9.21 6.71 5.26 2.5 62.1 5 1 1 14600.0 M20m Hydrogen 20 (A) 9 mELVTGKEY (S) 9 mELVTINYS m E/L|V|T|G K E\Y 44.4 4 4 4 3 0 dual b/y bias m:Oxidized methionine 30.728529 0.846334 543.2582 1069.523 15.9853 -8.9 -4.300000000000001 4.53 3659.0 nuORFdb_human ENST00000615206.4_1_12:56610319-56615392:- ENST00000615206.4_1_12:56610319-56615392:-|ENST00000345093.8_1_12:56610319-56615573:-|ENST00000552656.5_1_12:56610319-56615704:- RNF41 5' uORF 5' uORF GN=RNF41 5' uORF E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep3_Slot2-5_1_3521 40mio-rep4 E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep4_Slot2-5_1_3522.8974.1439.2 2 2 1 11.37 7.67 6.26 3.71 68.8 5 1 1 13000.0 M20m Hydrogen 20 (A) 9 mELVTGKEY (S) 9 mELVTINYS m E|L|V|T|G K E\Y 44.4 4 5 4 4 0 dual b/y bias m:Oxidized methionine 30.994951 0.851915 543.2603 1069.523 15.9893 -5.2 -0.5 4.53 3659.0 nuORFdb_human ENST00000615206.4_1_12:56610319-56615392:- ENST00000615206.4_1_12:56610319-56615392:-|ENST00000345093.8_1_12:56610319-56615573:-|ENST00000552656.5_1_12:56610319-56615704:- RNF41 5' uORF 5' uORF GN=RNF41 5' uORF E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep4_Slot2-5_1_3522 40mio-rep1 E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep1_Slot1-04_1_3496.22186.1220.2 2 1 1 9.5 1.49 4.82 8.0 67.8 6 1 1 3680.0 Hydrogen 13 (V) 11 MELYWNNGRSW (-) 11 LMEYATLnYYSS M E|L|Y|W/N\N/G R S W 45.5 5 4 5 3 0 unbiased 76.641182 0.988577 728.3241 1455.647 -0.0067 -4.6 -1.3999999999999995 5.75 2876.3 nuORFdb_human ENST00000429490.5_1_10:52193280-52220465:- ENST00000429490.5_1_10:52193280-52220465:- SGMS1 5' uORF 5' uORF GN=SGMS1 5' uORF E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep1_Slot1-04_1_3496 40mio-rep2 E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep2_Slot2-5_1_3520.22001.1233.2 2 2 1 10.67 7.61 10.67 66.7 8 1 1 4740.0 Hydrogen 13 (V) 11 MELYWNNGRSW (-) 11 M E/L|Y|W/N|N/G R/S\W 63.6 7 4 7 3 0 unbiased 76.003426 0.980714 728.3222 1455.647 -0.0107 -7.3 -2.3999999999999995 5.75 2876.3 nuORFdb_human ENST00000429490.5_1_10:52193280-52220465:- ENST00000429490.5_1_10:52193280-52220465:- SGMS1 5' uORF 5' uORF GN=SGMS1 5' uORF E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep2_Slot2-5_1_3520 40mio-rep3 E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep3_Slot2-5_1_3521.22032.1240.2 2 1 1 8.19 8.19 8.19 59.2 9 1 1 2370.0 Hydrogen 13 (V) 11 MELYWNNGRSW (-) 11 M E|L|Y|W/N\N/G/R/S\W 81.8 9 5 7 3 0 unbiased 76.108963 0.976087 728.324 1455.647 -0.0067 -4.6 0.0 5.75 2876.3 nuORFdb_human ENST00000429490.5_1_10:52193280-52220465:- ENST00000429490.5_1_10:52193280-52220465:- SGMS1 5' uORF 5' uORF GN=SGMS1 5' uORF E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep3_Slot2-5_1_3521 40mio-rep1 E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep1_Slot1-04_1_3496.19080.1210.2 2 2 1 9.53 9.53 9.53 58.3 7 1 1 9380.0 M13m Hydrogen 13 (V) 11 mELYWNNGRSW (-) 11 m E|L|Y|W\N/N/G R S\W 54.5 6 5 5 3 0 unbiased m:Oxidized methionine 65.912483 0.994657 736.3222 1455.647 15.9893 -3.8 -0.5999999999999996 5.75 2876.3 nuORFdb_human ENST00000429490.5_1_10:52193280-52220465:- ENST00000429490.5_1_10:52193280-52220465:- SGMS1 5' uORF 5' uORF GN=SGMS1 5' uORF E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep1_Slot1-04_1_3496 40mio-rep1 E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep1_Slot1-04_1_3496.17383.1428.2 2 1 1 12.4 7.36 6.3 5.04 75.2 7 0 0 25700.0 Hydrogen 1 (-) 9 MESPLGSHW (L) 9 MESLRmGFG M/E|S/P/L|G S/H\W 77.8 7 3 6 2 0 y-ion bias 60.045345 0.858927 522.2325 1043.461 -0.0028 -2.7 0.5 5.22 1269.5 nuORFdb_human ENST00000288235.8_1_15:59664829-59664865:- ENST00000288235.8_1_15:59664829-59664865:- MYO1E 5' uORF 5' uORF GN=MYO1E 5' uORF E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep1_Slot1-04_1_3496 40mio-rep2 E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep2_Slot2-5_1_3520.17044.1438.2 2 1 1 8.37 3.97 4.44 4.4 60.5 7 0 0 10500.0 Hydrogen 1 (-) 9 MESPLGSHW (L) 9 MESLRmGFG M/E|S/P/L|G\S H-W 77.8 7 3 5 2 1 unbiased 58.875725 0.85225 522.233 1043.461 -0.0028 -2.7 2.2 5.22 1269.5 nuORFdb_human ENST00000288235.8_1_15:59664829-59664865:- ENST00000288235.8_1_15:59664829-59664865:- MYO1E 5' uORF 5' uORF GN=MYO1E 5' uORF E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep2_Slot2-5_1_3520 5mio-rep1 E_20221201_NO30_400nL_HLAc1_5e6_directIP_titration_rep1_Slot1-37_1_3502.17491.1457.2 2 1 1 8.65 6.02 3.21 2.63 51.4 5 0 0 3090.0 Hydrogen 1 (-) 9 MESPLGSHW (L) 9 MESRFmVQ M/E|S|P L|G S H\W 44.4 4 4 4 3 0 dual b/y bias 60.416073 0.840423 522.2295 1043.461 -0.0108 -10.3 -3.3000000000000007 5.22 1269.5 nuORFdb_human ENST00000288235.8_1_15:59664829-59664865:- ENST00000288235.8_1_15:59664829-59664865:- MYO1E 5' uORF 5' uORF GN=MYO1E 5' uORF E_20221201_NO30_400nL_HLAc1_5e6_directIP_titration_rep1_Slot1-37_1_3502 10mio-rep1 E_20221201_NO30_400nL_HLAc1_1e7_directIP_titration_rep1_Slot2-3_1_3512.13787.1443.2 2 2 1 12.62 5.78 4.15 6.85 73.9 7 0 0 18000.0 M1m Hydrogen 1 (-) 9 mESPLGSHW (L) 9 EmSTEHRGP m E|S|P|L|G-S/H\W 77.8 7 5 5 4 1 dual b/y bias m:Oxidized methionine 47.629635 0.849547 530.2276 1043.461 15.9872 -7.3 0.40000000000000036 5.22 1269.5 nuORFdb_human ENST00000288235.8_1_15:59664829-59664865:- ENST00000288235.8_1_15:59664829-59664865:- MYO1E 5' uORF 5' uORF GN=MYO1E 5' uORF E_20221201_NO30_400nL_HLAc1_1e7_directIP_titration_rep1_Slot2-3_1_3512 10mio-rep2 E_20221201_NO30_400nL_HLAc1_1e7_directIP_titration_rep2_Slot2-3_1_3513.13750.1441.2 2 1 1 13.87 8.78 5.67 5.09 79.1 7 0 0 6810.0 M1m Hydrogen 1 (-) 9 mESPLGSHW (L) 9 mESWAVSHP m/E|S|P/L|G S/H\W 77.8 7 4 6 3 0 dual b/y bias m:Oxidized methionine 47.501297 0.850433 530.2279 1043.461 15.9872 -7.3 -0.5999999999999996 5.22 1269.5 nuORFdb_human ENST00000288235.8_1_15:59664829-59664865:- ENST00000288235.8_1_15:59664829-59664865:- MYO1E 5' uORF 5' uORF GN=MYO1E 5' uORF E_20221201_NO30_400nL_HLAc1_1e7_directIP_titration_rep2_Slot2-3_1_3513 10mio-rep3 E_20221201_NO30_400nL_HLAc1_1e7_directIP_titration_rep3_Slot2-3_1_3514.13699.1449.2 2 1 1 12.45 8.41 5.26 4.04 70.6 6 0 0 8100.0 M1m Hydrogen 1 (-) 9 mESPLGSHW (L) 9 mESWAVSHP m/E|S|P L|G S\H\W 55.6 5 5 4 3 0 dual b/y bias m:Oxidized methionine 47.327209 0.845795 530.2296 1043.461 15.9912 -3.5 2.8 5.22 1269.5 nuORFdb_human ENST00000288235.8_1_15:59664829-59664865:- ENST00000288235.8_1_15:59664829-59664865:- MYO1E 5' uORF 5' uORF GN=MYO1E 5' uORF E_20221201_NO30_400nL_HLAc1_1e7_directIP_titration_rep3_Slot2-3_1_3514 20mio-rep1 E_20221201_NO30_400nL_HLAc1_2e7_directIP_titration_rep1_Slot2-4_1_3515.13676.1430.2 2 1 1 14.41 9.22 6.2 5.2 71.2 7 0 0 9530.0 M1m Hydrogen 1 (-) 9 mESPLGSHW (L) 9 mESLRmGFG m/E|S|P/L|G S/H\W 77.8 7 4 6 3 0 dual b/y bias m:Oxidized methionine 47.235859 0.857683 530.2283 1043.461 15.9872 -7.3 -1.0999999999999996 5.22 1269.5 nuORFdb_human ENST00000288235.8_1_15:59664829-59664865:- ENST00000288235.8_1_15:59664829-59664865:- MYO1E 5' uORF 5' uORF GN=MYO1E 5' uORF E_20221201_NO30_400nL_HLAc1_2e7_directIP_titration_rep1_Slot2-4_1_3515 20mio-rep2 E_20221201_NO30_400nL_HLAc1_2e7_directIP_titration_rep3_Slot2-4_1_3518.13799.1439.2 2 1 1 11.13 7.44 4.21 3.69 76.6 6 0 0 12400.0 M1m Hydrogen 1 (-) 9 mESPLGSHW (L) 9 mESRFmVQ m/E|S|P/L|G S H-W 66.7 6 3 5 3 1 dual b/y bias m:Oxidized methionine 47.661755 0.852159 530.2284 1043.461 15.9872 -7.3 -1.0 5.22 1269.5 nuORFdb_human ENST00000288235.8_1_15:59664829-59664865:- ENST00000288235.8_1_15:59664829-59664865:- MYO1E 5' uORF 5' uORF GN=MYO1E 5' uORF E_20221201_NO30_400nL_HLAc1_2e7_directIP_titration_rep3_Slot2-4_1_3518 20mio-rep3 E_20221201_NO30_400nL_HLAc1_2e7_directIP_titration_rep4_Slot2-4_1_3519.13699.1431.2 2 1 1 10.45 5.05 5.74 5.4 64.9 6 0 0 7800.0 M1m Hydrogen 1 (-) 9 mESPLGSHW (L) 9 mESLRmGFG m E|S|P/L|G S/H\W 55.6 5 4 5 3 0 dual b/y bias m:Oxidized methionine 47.319054 0.857033 530.2289 1043.461 15.9892 -5.4 0.39999999999999947 5.22 1269.5 nuORFdb_human ENST00000288235.8_1_15:59664829-59664865:- ENST00000288235.8_1_15:59664829-59664865:- MYO1E 5' uORF 5' uORF GN=MYO1E 5' uORF E_20221201_NO30_400nL_HLAc1_2e7_directIP_titration_rep4_Slot2-4_1_3519 40mio-rep1 E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep1_Slot1-04_1_3496.13986.1400.2 2 2 1 16.04 7.99 5.92 8.05 80.6 7 0 0 32200.0 M1m Hydrogen 1 (-) 9 mESPLGSHW (L) 9 mESLRmGFG m/E|S|P/L|G S|H\W 77.8 7 5 6 4 0 dual b/y bias m:Oxidized methionine 48.312332 0.876234 530.2308 1043.461 15.9932 -1.6 1.6 5.22 1269.5 nuORFdb_human ENST00000288235.8_1_15:59664829-59664865:- ENST00000288235.8_1_15:59664829-59664865:- MYO1E 5' uORF 5' uORF GN=MYO1E 5' uORF E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep1_Slot1-04_1_3496 40mio-rep2 E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep2_Slot2-5_1_3520.13656.1413.2 2 2 1 11.54 6.63 4.91 4.91 73.3 7 0 0 36000.0 M1m Hydrogen 1 (-) 9 mESPLGSHW (L) 9 mESLRmGFG m/E|S|P/L|G S/H\W 77.8 7 4 6 3 0 dual b/y bias m:Oxidized methionine 47.174183 0.868425 530.2309 1043.461 15.9932 -1.6 3.3000000000000003 5.22 1269.5 nuORFdb_human ENST00000288235.8_1_15:59664829-59664865:- ENST00000288235.8_1_15:59664829-59664865:- MYO1E 5' uORF 5' uORF GN=MYO1E 5' uORF E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep2_Slot2-5_1_3520 40mio-rep3 E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep3_Slot2-5_1_3521.13664.1414.2 2 1 1 13.47 10.26 3.17 3.22 78.9 6 0 0 16700.0 M1m Hydrogen 1 (-) 9 mESPLGSHW (L) 9 mESLRmGFG m/E|S|P/L/G S H\W 66.7 6 3 5 2 0 unbiased m:Oxidized methionine 47.205208 0.867315 530.2292 1043.461 15.9892 -5.4 -0.8000000000000007 5.22 1269.5 nuORFdb_human ENST00000288235.8_1_15:59664829-59664865:- ENST00000288235.8_1_15:59664829-59664865:- MYO1E 5' uORF 5' uORF GN=MYO1E 5' uORF E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep3_Slot2-5_1_3521 40mio-rep4 E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep4_Slot2-5_1_3522.13686.1422.2 2 2 1 15.55 9.7 5.23 5.85 84.4 7 0 0 59300.0 M1m Hydrogen 1 (-) 9 mESPLGSHW (L) 9 mESWAVSHP m/E|S|P/L|G S/H\W 77.8 7 4 6 3 0 dual b/y bias m:Oxidized methionine 47.264977 0.862556 530.2295 1043.461 15.9892 -5.4 -0.7000000000000002 5.22 1269.5 nuORFdb_human ENST00000288235.8_1_15:59664829-59664865:- ENST00000288235.8_1_15:59664829-59664865:- MYO1E 5' uORF 5' uORF GN=MYO1E 5' uORF E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep4_Slot2-5_1_3522 5mio-rep1 E_20221201_NO30_400nL_HLAc1_5e6_directIP_titration_rep1_Slot1-37_1_3502.14048.1439.2 2 2 1 13.11 6.81 5.21 6.3 71.6 6 0 0 8090.0 M1m Hydrogen 1 (-) 9 mESPLGSHW (L) 9 mESLRmGFG m/E/S|P/L|G S H\W 66.7 6 3 5 2 0 unbiased m:Oxidized methionine 48.524239 0.851634 530.2286 1043.461 15.9892 -5.4 1.5999999999999996 5.22 1269.5 nuORFdb_human ENST00000288235.8_1_15:59664829-59664865:- ENST00000288235.8_1_15:59664829-59664865:- MYO1E 5' uORF 5' uORF GN=MYO1E 5' uORF E_20221201_NO30_400nL_HLAc1_5e6_directIP_titration_rep1_Slot1-37_1_3502 5mio-rep3 E_20221201_NO30_400nL_HLAc1_5e6_directIP_titration_rep3_Slot2-2_1_3511.13988.1443.2 2 2 1 9.51 5.25 4.01 4.26 67.7 6 0 0 8600.0 M1m Hydrogen 1 (-) 9 mESPLGSHW (L) 9 mESWAVSHP m/E|S|P/L|G S H\W 66.7 6 4 5 3 0 dual b/y bias m:Oxidized methionine 48.320244 0.849614 530.2271 1043.461 15.9852 -9.1 -0.9000000000000004 5.22 1269.5 nuORFdb_human ENST00000288235.8_1_15:59664829-59664865:- ENST00000288235.8_1_15:59664829-59664865:- MYO1E 5' uORF 5' uORF GN=MYO1E 5' uORF E_20221201_NO30_400nL_HLAc1_5e6_directIP_titration_rep3_Slot2-2_1_3511 40mio-rep1 E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep1_Slot1-04_1_3496.18451.480.1 1 2 1 9.67 1.45 6.88 8.22 80.0 6 0 0 8760.0 Hydrogen 37 (S) 9 MEVAAGTEW (R) 9 DATAGLMGEE M E V/A|A\G\T\E\W 66.7 6 5 2 1 0 b-ion bias 63.73616 1.437842 993.4341 993.435 -0.0006 -0.6 2.6 3.79 5533.2 nuORFdb_human ENST00000339995.9_2_11:10830403-10830559:- ENST00000339995.9_2_11:10830403-10830559:- EIF4G2 5' uORF 5' uORF GN=EIF4G2 5' uORF E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep1_Slot1-04_1_3496 40mio-rep2 E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep2_Slot2-5_1_3520.18124.502.1 1 2 1 10.68 2.59 7.59 8.09 77.1 7 0 0 5400.0 Hydrogen 37 (S) 9 MEVAAGTEW (R) 9 EGAAYPAEW M E/V/A/A|G\T|E\W 77.8 7 4 5 2 0 unbiased 62.608067 1.424761 993.4298 993.435 -0.0046 -4.6 0.3000000000000007 3.79 5533.2 nuORFdb_human ENST00000339995.9_2_11:10830403-10830559:- ENST00000339995.9_2_11:10830403-10830559:- EIF4G2 5' uORF 5' uORF GN=EIF4G2 5' uORF E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep2_Slot2-5_1_3520 40mio-rep4 E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep4_Slot2-5_1_3522.18138.498.1 1 1 1 10.56 0.66 8.86 9.91 78.1 7 0 0 3050.0 Hydrogen 37 (S) 9 MEVAAGTEW (R) 9 EPAGFSEAW M E/V/A/A|G\T\E\W 77.8 7 4 4 1 0 unbiased 62.639729 1.426884 993.429 993.435 -0.0056 -5.6 -0.8999999999999995 3.79 5533.2 nuORFdb_human ENST00000339995.9_2_11:10830403-10830559:- ENST00000339995.9_2_11:10830403-10830559:- EIF4G2 5' uORF 5' uORF GN=EIF4G2 5' uORF E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep4_Slot2-5_1_3522 10mio-rep1 E_20221201_NO30_400nL_HLAc1_1e7_directIP_titration_rep1_Slot2-3_1_3512.14389.513.1 1 1 1 8.55 5.0 8.55 60.2 7 0 0 1400.0 M37m Hydrogen 37 (S) 9 mEVAAGTEW (R) 9 m E/V/A|A\G\T\E\W 77.8 7 5 3 1 0 unbiased m:Oxidized methionine 49.707874 1.417936 1009.4172 993.435 15.9854 -9.4 -1.7000000000000002 3.79 5533.2 nuORFdb_human ENST00000339995.9_2_11:10830403-10830559:- ENST00000339995.9_2_11:10830403-10830559:- EIF4G2 5' uORF 5' uORF GN=EIF4G2 5' uORF E_20221201_NO30_400nL_HLAc1_1e7_directIP_titration_rep1_Slot2-3_1_3512 20mio-rep1 E_20221201_NO30_400nL_HLAc1_2e7_directIP_titration_rep1_Slot2-4_1_3515.14290.503.1 1 1 1 8.78 -1.79 6.83 10.57 62.9 7 0 0 1150.0 M37m Hydrogen 37 (S) 9 mEVAAGTEW (R) 9 SGGGSASSGGAGSA m E\V|A/A\G\T\E\W 77.8 7 6 2 1 0 b-ion bias m:Oxidized methionine 49.355335 1.423951 1009.4214 993.435 15.9854 -9.4 -3.2 3.79 5533.2 nuORFdb_human ENST00000339995.9_2_11:10830403-10830559:- ENST00000339995.9_2_11:10830403-10830559:- EIF4G2 5' uORF 5' uORF GN=EIF4G2 5' uORF E_20221201_NO30_400nL_HLAc1_2e7_directIP_titration_rep1_Slot2-4_1_3515 20mio-rep2 E_20221201_NO30_400nL_HLAc1_2e7_directIP_titration_rep3_Slot2-4_1_3518.14400.503.1 1 1 1 7.5 -0.7 6.26 8.2 63.9 6 0 0 1940.0 M37m Hydrogen 37 (S) 9 mEVAAGTEW (R) 9 TADLmQEW m E\V|A|A\G T\E\W 55.6 5 6 2 2 0 b-ion bias m:Oxidized methionine 49.739674 1.423893 1009.4239 993.435 15.9854 -9.4 -3.1000000000000005 3.79 5533.2 nuORFdb_human ENST00000339995.9_2_11:10830403-10830559:- ENST00000339995.9_2_11:10830403-10830559:- EIF4G2 5' uORF 5' uORF GN=EIF4G2 5' uORF E_20221201_NO30_400nL_HLAc1_2e7_directIP_titration_rep3_Slot2-4_1_3518 40mio-rep2 E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep2_Slot2-5_1_3520.14202.500.1 1 3 1 15.62 4.68 10.33 10.94 85.6 7 0 0 11700.0 M37m Hydrogen 37 (S) 9 mEVAAGTEW (R) 9 TADLmQEW m E/V|A|A\G|T\E\W 77.8 7 6 4 3 0 dual b/y bias m:Oxidized methionine 49.059765 1.425913 1009.418 993.435 15.9854 -9.4 -4.5 3.79 5533.2 nuORFdb_human ENST00000339995.9_2_11:10830403-10830559:- ENST00000339995.9_2_11:10830403-10830559:- EIF4G2 5' uORF 5' uORF GN=EIF4G2 5' uORF E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep2_Slot2-5_1_3520 10mio-rep1 E_20221201_NO30_400nL_HLAc1_1e7_directIP_titration_rep1_Slot2-3_1_3512.17094.522.1 1 1 1 7.44 4.08 5.54 3.36 61.2 7 0 0 3770.0 Hydrogen 1 (-) 9 MEVASVAAY (C) 9 QNTSVSAAY M E\V\A|S\V\A\A\Y 77.8 7 7 1 1 0 b-ion bias 59.049496 1.41272 940.4328 940.444 -0.0114 -12.2 -4.499999999999999 4.0 5788.6 smORF_v4|nuORFdb_human PPP1R15B_1:204380615-204380770:- PPP1R15B_1:204380615-204380770:-|ENST00000367188.4_1_1:204380617-204380770:- PPP1R15B uORF 1:204380615-204380770:- 1 PPP1R15B 5' uORF 5' uORF GN=PPP1R15B E_20221201_NO30_400nL_HLAc1_1e7_directIP_titration_rep1_Slot2-3_1_3512 10mio-rep2 E_20221201_NO30_400nL_HLAc1_1e7_directIP_titration_rep2_Slot2-3_1_3513.17026.530.1 1 1 1 7.19 2.46 5.16 4.73 65.9 6 0 0 2920.0 Hydrogen 1 (-) 9 MEVASVAAY (C) 9 QNTSVSAAY M E V\A|S\V\A\A\Y 66.7 6 6 1 1 0 b-ion bias 58.813484 1.408043 940.4393 940.444 -0.0054 -5.8 0.9000000000000004 4.0 5788.6 smORF_v4|nuORFdb_human PPP1R15B_1:204380615-204380770:- PPP1R15B_1:204380615-204380770:-|ENST00000367188.4_1_1:204380617-204380770:- PPP1R15B uORF 1:204380615-204380770:- 1 PPP1R15B 5' uORF 5' uORF GN=PPP1R15B E_20221201_NO30_400nL_HLAc1_1e7_directIP_titration_rep2_Slot2-3_1_3513 10mio-rep3 E_20221201_NO30_400nL_HLAc1_1e7_directIP_titration_rep3_Slot2-3_1_3514.16984.524.1 1 1 1 9.49 3.76 5.36 5.73 69.1 6 0 0 2280.0 Hydrogen 1 (-) 9 MEVASVAAY (C) 9 QNTSVSAAY M E V\A|S\V\A\A\Y 66.7 6 6 1 1 0 b-ion bias 58.668819 1.411451 940.4396 940.444 -0.0044 -4.7 1.5999999999999996 4.0 5788.6 smORF_v4|nuORFdb_human PPP1R15B_1:204380615-204380770:- PPP1R15B_1:204380615-204380770:-|ENST00000367188.4_1_1:204380617-204380770:- PPP1R15B uORF 1:204380615-204380770:- 1 PPP1R15B 5' uORF 5' uORF GN=PPP1R15B E_20221201_NO30_400nL_HLAc1_1e7_directIP_titration_rep3_Slot2-3_1_3514 20mio-rep1 E_20221201_NO30_400nL_HLAc1_2e7_directIP_titration_rep1_Slot2-4_1_3515.16957.522.1 1 1 1 7.64 3.51 3.47 4.13 60.5 6 0 0 5970.0 Hydrogen 1 (-) 9 MEVASVAAY (C) 9 FPDEKAAY M E V|A\S\V\A\A\Y 66.7 6 6 1 1 0 b-ion bias 58.561756 1.412599 940.437 940.444 -0.0074 -7.9 -1.7000000000000002 4.0 5788.6 smORF_v4|nuORFdb_human PPP1R15B_1:204380615-204380770:- PPP1R15B_1:204380615-204380770:-|ENST00000367188.4_1_1:204380617-204380770:- PPP1R15B uORF 1:204380615-204380770:- 1 PPP1R15B 5' uORF 5' uORF GN=PPP1R15B E_20221201_NO30_400nL_HLAc1_2e7_directIP_titration_rep1_Slot2-4_1_3515 20mio-rep3 E_20221201_NO30_400nL_HLAc1_2e7_directIP_titration_rep4_Slot2-4_1_3519.16974.511.1 1 2 1 10.53 4.5 6.63 6.04 69.6 7 0 0 5890.0 Hydrogen 1 (-) 9 MEVASVAAY (C) 9 QNTSVSAAY M E\V\A|S\V|A\A\Y 77.8 7 7 2 2 0 b-ion bias 58.628174 1.41929 940.437 940.444 -0.0074 -7.9 -2.1000000000000005 4.0 5788.6 smORF_v4|nuORFdb_human PPP1R15B_1:204380615-204380770:- PPP1R15B_1:204380615-204380770:-|ENST00000367188.4_1_1:204380617-204380770:- PPP1R15B uORF 1:204380615-204380770:- 1 PPP1R15B 5' uORF 5' uORF GN=PPP1R15B E_20221201_NO30_400nL_HLAc1_2e7_directIP_titration_rep4_Slot2-4_1_3519 40mio-rep1 E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep1_Slot1-04_1_3496.17239.496.1 1 2 1 9.9 3.18 5.94 6.72 73.4 6 0 0 66200.0 Hydrogen 1 (-) 9 MEVASVAAY (C) 9 QNTSVSAAY M E V\A|S\V\A\A\Y 66.7 6 6 1 1 0 b-ion bias 59.548203 1.428181 940.4433 940.444 -0.0014 -1.5 1.7000000000000002 4.0 5788.6 smORF_v4|nuORFdb_human PPP1R15B_1:204380615-204380770:- PPP1R15B_1:204380615-204380770:-|ENST00000367188.4_1_1:204380617-204380770:- PPP1R15B uORF 1:204380615-204380770:- 1 PPP1R15B 5' uORF 5' uORF GN=PPP1R15B E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep1_Slot1-04_1_3496 40mio-rep2 E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep2_Slot2-5_1_3520.16913.511.1 1 1 1 10.77 4.19 5.87 6.59 70.8 7 0 0 7880.0 Hydrogen 1 (-) 9 MEVASVAAY (C) 9 QNTSVSAAY M E/V|A|S\V\A\A\Y 77.8 7 6 3 2 0 b-ion bias 58.423412 1.419448 940.4396 940.444 -0.0044 -4.7 0.20000000000000018 4.0 5788.6 smORF_v4|nuORFdb_human PPP1R15B_1:204380615-204380770:- PPP1R15B_1:204380615-204380770:-|ENST00000367188.4_1_1:204380617-204380770:- PPP1R15B uORF 1:204380615-204380770:- 1 PPP1R15B 5' uORF 5' uORF GN=PPP1R15B E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep2_Slot2-5_1_3520 40mio-rep3 E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep3_Slot2-5_1_3521.16942.510.1 1 2 1 8.47 4.67 4.05 3.8 68.0 6 0 0 26200.0 Hydrogen 1 (-) 9 MEVASVAAY (C) 9 FPDEKAAY M E V/A|S\V\A\A\Y 66.7 6 5 2 1 0 b-ion bias 58.524357 1.420262 940.4391 940.444 -0.0054 -5.8 -1.2000000000000002 4.0 5788.6 smORF_v4|nuORFdb_human PPP1R15B_1:204380615-204380770:- PPP1R15B_1:204380615-204380770:-|ENST00000367188.4_1_1:204380617-204380770:- PPP1R15B uORF 1:204380615-204380770:- 1 PPP1R15B 5' uORF 5' uORF GN=PPP1R15B E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep3_Slot2-5_1_3521 40mio-rep4 E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep4_Slot2-5_1_3522.16902.509.1 1 1 1 8.04 1.25 5.63 6.79 67.3 6 0 0 6050.0 Hydrogen 1 (-) 9 MEVASVAAY (C) 9 QNTSVSAAY M E V\A\S|V\A\A\Y 66.7 6 6 1 1 0 b-ion bias 58.370613 1.420464 940.4417 940.444 -0.0024 -2.6 2.1 4.0 5788.6 smORF_v4|nuORFdb_human PPP1R15B_1:204380615-204380770:- PPP1R15B_1:204380615-204380770:-|ENST00000367188.4_1_1:204380617-204380770:- PPP1R15B uORF 1:204380615-204380770:- 1 PPP1R15B 5' uORF 5' uORF GN=PPP1R15B E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep4_Slot2-5_1_3522 10mio-rep1 E_20221201_NO30_400nL_HLAc1_1e7_directIP_titration_rep1_Slot2-3_1_3512.13029.512.1 1 2 1 9.0 2.61 4.38 6.39 68.2 6 0 0 9280.0 M1m Hydrogen 1 (-) 9 mEVASVAAY (C) 9 SmLnLGAFS m E V\A\S\V\A\A\Y 66.7 6 6 0 0 0 b-ion bias m:Oxidized methionine 45.013111 1.418732 956.4297 940.444 15.9856 -9.8 -2.1000000000000005 4.0 5788.6 smORF_v4|nuORFdb_human PPP1R15B_1:204380615-204380770:- PPP1R15B_1:204380615-204380770:-|ENST00000367188.4_1_1:204380617-204380770:- PPP1R15B uORF 1:204380615-204380770:- 1 PPP1R15B 5' uORF 5' uORF GN=PPP1R15B E_20221201_NO30_400nL_HLAc1_1e7_directIP_titration_rep1_Slot2-3_1_3512 10mio-rep2 E_20221201_NO30_400nL_HLAc1_1e7_directIP_titration_rep2_Slot2-3_1_3513.13069.527.1 1 4 1 10.38 1.59 7.06 8.79 78.3 7 0 0 11500.0 M1m Hydrogen 1 (-) 9 mEVASVAAY (C) 9 AELGmVSAY m E|V|A\S\V\A\A\Y 77.8 7 7 2 2 0 b-ion bias m:Oxidized methionine 45.150333 1.409707 956.4328 940.444 15.9886 -6.6 0.10000000000000053 4.0 5788.6 smORF_v4|nuORFdb_human PPP1R15B_1:204380615-204380770:- PPP1R15B_1:204380615-204380770:-|ENST00000367188.4_1_1:204380617-204380770:- PPP1R15B uORF 1:204380615-204380770:- 1 PPP1R15B 5' uORF 5' uORF GN=PPP1R15B E_20221201_NO30_400nL_HLAc1_1e7_directIP_titration_rep2_Slot2-3_1_3513 10mio-rep3 E_20221201_NO30_400nL_HLAc1_1e7_directIP_titration_rep3_Slot2-3_1_3514.13036.531.1 1 4 1 13.37 2.08 8.42 11.29 83.9 7 0 0 13000.0 M1m Hydrogen 1 (-) 9 mEVASVAAY (C) 9 KEAVEmAY m E|V|A|S\V\A\A\Y 77.8 7 7 3 3 0 dual b/y bias m:Oxidized methionine 45.038139 1.407775 956.4304 940.444 15.9856 -9.8 -3.500000000000001 4.0 5788.6 smORF_v4|nuORFdb_human PPP1R15B_1:204380615-204380770:- PPP1R15B_1:204380615-204380770:-|ENST00000367188.4_1_1:204380617-204380770:- PPP1R15B uORF 1:204380615-204380770:- 1 PPP1R15B 5' uORF 5' uORF GN=PPP1R15B E_20221201_NO30_400nL_HLAc1_1e7_directIP_titration_rep3_Slot2-3_1_3514 20mio-rep1 E_20221201_NO30_400nL_HLAc1_2e7_directIP_titration_rep1_Slot2-4_1_3515.12919.513.1 1 5 1 10.99 2.3 6.37 8.69 80.6 7 0 0 19800.0 M1m Hydrogen 1 (-) 9 mEVASVAAY (C) 9 GISSMDIAY m E|V|A\S\V\A\A\Y 77.8 7 7 2 2 0 b-ion bias m:Oxidized methionine 44.62188 1.418399 956.4358 940.444 15.9916 -3.5 2.7 4.0 5788.6 smORF_v4|nuORFdb_human PPP1R15B_1:204380615-204380770:- PPP1R15B_1:204380615-204380770:-|ENST00000367188.4_1_1:204380617-204380770:- PPP1R15B uORF 1:204380615-204380770:- 1 PPP1R15B 5' uORF 5' uORF GN=PPP1R15B E_20221201_NO30_400nL_HLAc1_2e7_directIP_titration_rep1_Slot2-4_1_3515 20mio-rep3 E_20221201_NO30_400nL_HLAc1_2e7_directIP_titration_rep4_Slot2-4_1_3519.12953.497.1 1 4 1 10.23 2.0 5.29 8.23 75.7 7 0 0 15500.0 M1m Hydrogen 1 (-) 9 mEVASVAAY (C) 9 mDIKEASF m E\V|A\S\V\A\A\Y 77.8 7 7 1 1 0 b-ion bias m:Oxidized methionine 44.744202 1.427804 956.4341 940.444 15.9896 -5.6 0.20000000000000018 4.0 5788.6 smORF_v4|nuORFdb_human PPP1R15B_1:204380615-204380770:- PPP1R15B_1:204380615-204380770:-|ENST00000367188.4_1_1:204380617-204380770:- PPP1R15B uORF 1:204380615-204380770:- 1 PPP1R15B 5' uORF 5' uORF GN=PPP1R15B E_20221201_NO30_400nL_HLAc1_2e7_directIP_titration_rep4_Slot2-4_1_3519 40mio-rep1 E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep1_Slot1-04_1_3496.13209.488.1 1 4 1 12.58 3.54 6.83 9.04 81.8 7 0 0 27000.0 M1m Hydrogen 1 (-) 9 mEVASVAAY (C) 9 KEAVEmAY m E\V|A|S\V\A\A\Y 77.8 7 7 2 2 0 b-ion bias m:Oxidized methionine 45.628429 1.433165 956.439 940.444 15.9946 -0.4 2.8000000000000003 4.0 5788.6 smORF_v4|nuORFdb_human PPP1R15B_1:204380615-204380770:- PPP1R15B_1:204380615-204380770:-|ENST00000367188.4_1_1:204380617-204380770:- PPP1R15B uORF 1:204380615-204380770:- 1 PPP1R15B 5' uORF 5' uORF GN=PPP1R15B E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep1_Slot1-04_1_3496 40mio-rep2 E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep2_Slot2-5_1_3520.12915.491.1 1 4 1 10.27 2.41 5.95 7.86 73.8 7 0 0 15000.0 M1m Hydrogen 1 (-) 9 mEVASVAAY (C) 9 AELGmVSAY m E\V|A|S\V\A\A\Y 77.8 7 7 2 2 0 b-ion bias m:Oxidized methionine 44.614742 1.431436 956.4329 940.444 15.9886 -6.6 -1.6999999999999993 4.0 5788.6 smORF_v4|nuORFdb_human PPP1R15B_1:204380615-204380770:- PPP1R15B_1:204380615-204380770:-|ENST00000367188.4_1_1:204380617-204380770:- PPP1R15B uORF 1:204380615-204380770:- 1 PPP1R15B 5' uORF 5' uORF GN=PPP1R15B E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep2_Slot2-5_1_3520 40mio-rep3 E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep3_Slot2-5_1_3521.12995.511.1 1 5 1 12.19 5.4 4.49 6.79 80.9 6 0 0 23700.0 M1m Hydrogen 1 (-) 9 mEVASVAAY (C) 9 KEAVEmAY m E V\A\S\V\A\A\Y 66.7 6 6 0 0 0 b-ion bias m:Oxidized methionine 44.895306 1.419542 956.4345 940.444 15.9896 -5.6 -1.0 4.0 5788.6 smORF_v4|nuORFdb_human PPP1R15B_1:204380615-204380770:- PPP1R15B_1:204380615-204380770:-|ENST00000367188.4_1_1:204380617-204380770:- PPP1R15B uORF 1:204380615-204380770:- 1 PPP1R15B 5' uORF 5' uORF GN=PPP1R15B E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep3_Slot2-5_1_3521 40mio-rep4 E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep4_Slot2-5_1_3522.12991.514.1 1 3 1 10.17 2.32 5.69 7.85 77.3 7 0 0 9960.0 M1m Hydrogen 1 (-) 9 mEVASVAAY (C) 9 mDIKEASF m E/V|A\S\V\A\A\Y 77.8 7 6 2 1 0 b-ion bias m:Oxidized methionine 44.861931 1.417699 956.4351 940.444 15.9906 -4.6 0.10000000000000053 4.0 5788.6 smORF_v4|nuORFdb_human PPP1R15B_1:204380615-204380770:- PPP1R15B_1:204380615-204380770:-|ENST00000367188.4_1_1:204380617-204380770:- PPP1R15B uORF 1:204380615-204380770:- 1 PPP1R15B 5' uORF 5' uORF GN=PPP1R15B E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep4_Slot2-5_1_3522 5mio-rep2 E_20221201_NO30_400nL_HLAc1_5e6_directIP_titration_rep2_Slot2-2_1_3510.13198.515.1 1 2 1 9.26 2.44 4.99 6.82 76.5 7 0 0 5590.0 M1m Hydrogen 1 (-) 9 mEVASVAAY (C) 9 AELGmVSAY m E\V\A\S\V\A\A\Y 77.8 7 7 0 0 0 b-ion bias m:Oxidized methionine 45.584274 1.417239 956.4306 940.444 15.9866 -8.7 0.0 4.0 5788.6 smORF_v4|nuORFdb_human PPP1R15B_1:204380615-204380770:- PPP1R15B_1:204380615-204380770:-|ENST00000367188.4_1_1:204380617-204380770:- PPP1R15B uORF 1:204380615-204380770:- 1 PPP1R15B 5' uORF 5' uORF GN=PPP1R15B E_20221201_NO30_400nL_HLAc1_5e6_directIP_titration_rep2_Slot2-2_1_3510 40mio-rep1 E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep1_Slot1-04_1_3496.13018.1395.2 2 1 1 13.7 6.95 6.84 6.75 82.7 7 1 1 8490.0 Hydrogen 2 (M) 9 MEVIQKNSF (A) 9 MEVImKTLn M/E|V|I|Q|K N\S\F 77.8 7 6 5 4 0 dual b/y bias 44.96891 0.879279 548.2762 1095.55 -0.0056 -5.1 -1.8999999999999995 5.75 3804.6 nuORFdb_human ENST00000359242.7_2_1:86851271-86852694:- ENST00000359242.7_2_1:86851271-86852694:-|ENST00000478286.2_1_1:86852553-86852694:-|ENST00000488879.6_1_1:86850449-86852694:- ODF2L Out-of-Frame Out-of-Frame GN=ODF2L Out-of-Frame E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep1_Slot1-04_1_3496 40mio-rep3 E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep3_Slot2-5_1_3521.12721.1406.2 2 2 1 12.74 5.25 6.51 7.49 78.0 8 1 1 14200.0 Hydrogen 2 (M) 9 MEVIQKNSF (A) 9 MEVImKTLn M/E|V|I/Q|K\N\S\F 100.0 9 6 5 3 0 dual b/y bias 43.948727 0.872812 548.2771 1095.55 -0.0036 -3.3 1.2999999999999998 5.75 3804.6 nuORFdb_human ENST00000359242.7_2_1:86851271-86852694:- ENST00000359242.7_2_1:86851271-86852694:-|ENST00000478286.2_1_1:86852553-86852694:-|ENST00000488879.6_1_1:86850449-86852694:- ODF2L Out-of-Frame Out-of-Frame GN=ODF2L Out-of-Frame E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep3_Slot2-5_1_3521 40mio-rep4 E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep4_Slot2-5_1_3522.12712.1420.2 2 2 1 9.8 3.44 6.8 6.36 72.9 6 1 1 15500.0 Hydrogen 2 (M) 9 MEVIQKNSF (A) 9 MEVImKTLn M E|V|I|Q|K N\S\F 55.6 5 6 4 4 0 dual b/y bias 43.898731 0.863539 548.2774 1095.55 -0.0036 -3.3 1.4000000000000004 5.75 3804.6 nuORFdb_human ENST00000359242.7_2_1:86851271-86852694:- ENST00000359242.7_2_1:86851271-86852694:-|ENST00000478286.2_1_1:86852553-86852694:-|ENST00000488879.6_1_1:86850449-86852694:- ODF2L Out-of-Frame Out-of-Frame GN=ODF2L Out-of-Frame E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep4_Slot2-5_1_3522 40mio-rep3 E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep3_Slot2-5_1_3521.9311.1428.2 2 1 1 8.87 6.72 8.87 59.5 7 1 1 9480.0 M2m Hydrogen 2 (M) 9 mEVIQKNSF (A) 9 m E|V|I|Q/K\N\S\F 77.8 7 6 4 3 0 dual b/y bias m:Oxidized methionine 32.167763 0.858742 556.2732 1095.55 15.9884 -5.8 -1.2000000000000002 5.75 3804.6 nuORFdb_human ENST00000359242.7_2_1:86851271-86852694:- ENST00000359242.7_2_1:86851271-86852694:-|ENST00000478286.2_1_1:86852553-86852694:-|ENST00000488879.6_1_1:86850449-86852694:- ODF2L Out-of-Frame Out-of-Frame GN=ODF2L Out-of-Frame E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep3_Slot2-5_1_3521 40mio-rep4 E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep4_Slot2-5_1_3522.9328.1421.2 2 1 1 11.12 6.66 6.9 4.45 71.3 6 1 1 7530.0 M2m Hydrogen 2 (M) 9 mEVIQKNSF (A) 9 LDmLTENLF m E/V|I|Q/K N\S\F 55.6 5 4 4 2 0 unbiased m:Oxidized methionine 32.216759 0.863215 556.2717 1095.55 15.9864 -7.6 -2.8999999999999995 5.75 3804.6 nuORFdb_human ENST00000359242.7_2_1:86851271-86852694:- ENST00000359242.7_2_1:86851271-86852694:-|ENST00000478286.2_1_1:86852553-86852694:-|ENST00000488879.6_1_1:86850449-86852694:- ODF2L Out-of-Frame Out-of-Frame GN=ODF2L Out-of-Frame E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep4_Slot2-5_1_3522 40mio-rep1 E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep1_Slot1-04_1_3496.10511.1511.3 3 1 1 7.51 4.19 3.33 3.32 54.1 5 1 2 11700.0 Hydrogen 6 (N) 11 MEYFVPHTKRY (-) 11 MEYLLNQNGLIY M/E|Y|F/V/P H T K R Y 45.5 5 2 5 2 0 y-ion bias 36.310555 0.806614 490.909 1470.72 -0.0074 -5.0 -1.7999999999999998 8.27 2030.3 nuORFdb_human ENST00000419821.6_1_12:118693456-118704503:- ENST00000419821.6_1_12:118693456-118704503:- TAOK3 5' uORF 5' uORF GN=TAOK3 5' uORF E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep1_Slot1-04_1_3496 40mio-rep1 E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep1_Slot1-04_1_3496.8557.1555.3 3 1 1 11.28 4.85 6.43 6.43 70.0 6 1 2 9890.0 M6m Hydrogen 6 (N) 11 mEYFVPHTKRY (-) 11 mEYLLNQNGLIY m/E|Y|F|V/P H/T K R Y 45.5 5 3 6 3 0 y-ion bias m:Oxidized methionine 29.563948 0.778814 496.2422 1470.72 15.9916 -2.2 1.0 8.27 2030.3 nuORFdb_human ENST00000419821.6_1_12:118693456-118704503:- ENST00000419821.6_1_12:118693456-118704503:- TAOK3 5' uORF 5' uORF GN=TAOK3 5' uORF E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep1_Slot1-04_1_3496 40mio-rep2 E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep2_Slot2-5_1_3520.8284.1565.3 3 1 1 9.72 4.29 5.17 5.43 59.2 7 1 2 5850.0 M6m Hydrogen 6 (N) 11 mEYFVPHTKRY (-) 11 EYRDIRLmVmE m E/Y|F/V/P/H/T/K R Y 54.5 6 1 7 1 0 y-ion bias m:Oxidized methionine 28.62101 0.772205 496.2395 1470.72 15.9826 -8.3 -3.4000000000000004 8.27 2030.3 nuORFdb_human ENST00000419821.6_1_12:118693456-118704503:- ENST00000419821.6_1_12:118693456-118704503:- TAOK3 5' uORF 5' uORF GN=TAOK3 5' uORF E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep2_Slot2-5_1_3520 40mio-rep3 E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep3_Slot2-5_1_3521.8306.1566.3 3 1 1 10.88 2.96 7.31 7.92 64.1 6 1 2 5560.0 M6m Hydrogen 6 (N) 11 mEYFVPHTKRY (-) 11 YmVIYLGRTPEE m/E|Y/F/V|P/H T K R Y 54.5 6 2 6 2 0 y-ion bias m:Oxidized methionine 28.693449 0.771776 496.2389 1470.72 15.9826 -8.3 -3.700000000000001 8.27 2030.3 nuORFdb_human ENST00000419821.6_1_12:118693456-118704503:- ENST00000419821.6_1_12:118693456-118704503:- TAOK3 5' uORF 5' uORF GN=TAOK3 5' uORF E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep3_Slot2-5_1_3521 SCP E_20221201_NO30_400nL_HLAc1_4e7_PDAC_Slot2-6_1_3524.25923.346.1 1 1 1 9.26 3.64 6.91 5.63 73.4 6 0 0 13900.0 Hydrogen 48 (F) 9 MFDAVVTQL (-) 9 MYQVVQTI M F D\A|V|V\T\Q-L 66.7 6 5 2 2 1 b-ion bias 89.555962 1.517292 1023.5176 1023.518 0.0021 2.0 4.4 3.8 6487.6 nuORFdb_human ENST00000374794.7_2_10:34759149-34806035:- ENST00000374794.7_2_10:34759149-34806035:- PARD3 Out-of-Frame Out-of-Frame GN=PARD3 Out-of-Frame E_20221201_NO30_400nL_HLAc1_4e7_PDAC_Slot2-6_1_3524 20mio-rep1 E_20221201_NO30_400nL_HLAc1_2e7_directIP_titration_rep1_Slot2-4_1_3515.18161.1344.2 2 2 1 7.78 2.99 7.78 52.6 7 1 1 9340.0 M20m Hydrogen 15 (K) 9 MFFTRmQSF (L) 9 M/F/F/T R|m\Q\S\F 77.8 7 4 4 1 0 unbiased m:Oxidized methionine 62.720604 0.911672 605.7688 1194.543 15.9873 -6.3 -0.09999999999999964 9.5 3141.8 nuORFdb_human ENST00000372714.5_1_10:76586522-76602417:+ ENST00000372714.5_1_10:76586522-76602417:+|ENST00000372724.5_1_10:76598507-76602417:+ KAT6B 5' uORF 5' uORF GN=KAT6B 5' uORF E_20221201_NO30_400nL_HLAc1_2e7_directIP_titration_rep1_Slot2-4_1_3515 20mio-rep2 E_20221201_NO30_400nL_HLAc1_2e7_directIP_titration_rep3_Slot2-4_1_3518.18222.1342.2 2 2 1 8.15 1.47 6.67 6.67 61.9 6 1 1 7180.0 M20m Hydrogen 15 (K) 9 MFFTRmQSF (L) 9 MFSMRLHCE M/F|F|T|R m Q\S\F 66.7 6 5 4 3 0 dual b/y bias m:Oxidized methionine 62.940628 0.912797 605.7706 1194.543 15.9913 -3.0 3.3 9.5 3141.8 nuORFdb_human ENST00000372714.5_1_10:76586522-76602417:+ ENST00000372714.5_1_10:76586522-76602417:+|ENST00000372724.5_1_10:76598507-76602417:+ KAT6B 5' uORF 5' uORF GN=KAT6B 5' uORF E_20221201_NO30_400nL_HLAc1_2e7_directIP_titration_rep3_Slot2-4_1_3518 40mio-rep1 E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep1_Slot1-04_1_3496.18297.1315.2 2 4 1 13.82 1.69 1.1 12.13 66.5 7 1 1 28200.0 M20m Hydrogen 15 (K) 9 MFFTRmQSF (L) 9 MEKMFSFSGF M/F|F|T/R\m Q\S\F 77.8 7 5 4 2 0 unbiased m:Oxidized methionine 63.204395 0.929638 605.7713 1194.543 15.9913 -3.0 0.20000000000000018 9.5 3141.8 nuORFdb_human ENST00000372714.5_1_10:76586522-76602417:+ ENST00000372714.5_1_10:76586522-76602417:+|ENST00000372724.5_1_10:76598507-76602417:+ KAT6B 5' uORF 5' uORF GN=KAT6B 5' uORF E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep1_Slot1-04_1_3496 40mio-rep1 E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep1_Slot1-04_1_3496.19970.1331.2 2 3 1 12.74 3.44 3.51 9.3 69.6 7 1 1 27900.0 M15m Hydrogen 15 (K) 9 mFFTRMQSF (L) 9 FmFGSmFLQA m F|F|T/R\M|Q|S\F 77.8 7 6 5 4 0 dual b/y bias m:Oxidized methionine 68.986069 0.919359 605.7705 1194.543 15.9893 -4.7 -1.5 9.5 3141.8 nuORFdb_human ENST00000372714.5_1_10:76586522-76602417:+ ENST00000372714.5_1_10:76586522-76602417:+|ENST00000372724.5_1_10:76598507-76602417:+ KAT6B 5' uORF 5' uORF GN=KAT6B 5' uORF E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep1_Slot1-04_1_3496 40mio-rep3 E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep3_Slot2-5_1_3521.19878.1360.2 2 1 1 12.12 3.69 2.95 8.43 67.2 7 1 1 5010.0 M15m Hydrogen 15 (K) 9 mFFTRMQSF (L) 9 FmATISYFGAS m F|F/T/R\M\Q|S\F 77.8 7 5 4 2 0 unbiased m:Oxidized methionine 68.668068 0.901614 605.7696 1194.543 15.9893 -4.7 -0.10000000000000053 9.5 3141.8 nuORFdb_human ENST00000372714.5_1_10:76586522-76602417:+ ENST00000372714.5_1_10:76586522-76602417:+|ENST00000372724.5_1_10:76598507-76602417:+ KAT6B 5' uORF 5' uORF GN=KAT6B 5' uORF E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep3_Slot2-5_1_3521 40mio-rep4 E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep4_Slot2-5_1_3522.19872.1357.2 2 1 1 9.95 2.66 3.43 7.29 62.3 7 1 1 5490.0 M15m Hydrogen 15 (K) 9 mFFTRMQSF (L) 9 FmFGSmFLQA m F/F/T|R/M\Q\S\F 77.8 7 4 4 1 0 unbiased m:Oxidized methionine 68.631134 0.903418 605.7717 1194.543 15.9933 -1.4 3.3000000000000003 9.5 3141.8 nuORFdb_human ENST00000372714.5_1_10:76586522-76602417:+ ENST00000372714.5_1_10:76586522-76602417:+|ENST00000372724.5_1_10:76598507-76602417:+ KAT6B 5' uORF 5' uORF GN=KAT6B 5' uORF E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep4_Slot2-5_1_3522 10mio-rep1 E_20221201_NO30_400nL_HLAc1_1e7_directIP_titration_rep1_Slot2-3_1_3512.15181.1356.2 2 2 1 12.62 5.12 2.16 7.5 75.2 8 1 1 12400.0 M15m M20m Hydrogen 15 (K) 9 mFFTRmQSF (L) 9 YMKVmGGYDF m/F|F/T/R/m\Q|S\F 100.0 9 4 6 2 0 unbiased m:Oxidized methionine 52.444252 0.903606 613.765 1194.543 31.9793 -8.6 -0.8999999999999995 9.5 3141.8 nuORFdb_human ENST00000372714.5_1_10:76586522-76602417:+ ENST00000372714.5_1_10:76586522-76602417:+|ENST00000372724.5_1_10:76598507-76602417:+ KAT6B 5' uORF 5' uORF GN=KAT6B 5' uORF E_20221201_NO30_400nL_HLAc1_1e7_directIP_titration_rep1_Slot2-3_1_3512 10mio-rep2 E_20221201_NO30_400nL_HLAc1_1e7_directIP_titration_rep2_Slot2-3_1_3513.15218.1368.2 2 2 1 13.96 2.81 2.31 11.15 65.0 7 1 1 10200.0 M15m M20m Hydrogen 15 (K) 9 mFFTRmQSF (L) 9 FmmELTAHQS m F|F|T|R/m\Q\S\F 77.8 7 6 4 3 0 dual b/y bias m:Oxidized methionine 52.569744 0.896354 613.7648 1194.543 31.9793 -8.6 -1.8999999999999995 9.5 3141.8 nuORFdb_human ENST00000372714.5_1_10:76586522-76602417:+ ENST00000372714.5_1_10:76586522-76602417:+|ENST00000372724.5_1_10:76598507-76602417:+ KAT6B 5' uORF 5' uORF GN=KAT6B 5' uORF E_20221201_NO30_400nL_HLAc1_1e7_directIP_titration_rep2_Slot2-3_1_3513 10mio-rep3 E_20221201_NO30_400nL_HLAc1_1e7_directIP_titration_rep3_Slot2-3_1_3514.15185.1367.2 2 3 1 11.32 3.72 1.42 7.6 66.9 6 1 1 12600.0 M15m M20m Hydrogen 15 (K) 9 mFFTRmQSF (L) 9 mFSMRLHCE m F|F|T/R m/Q|S\F 55.6 5 4 5 3 0 dual b/y bias m:Oxidized methionine 52.458141 0.896995 613.764 1194.543 31.9773 -10.2 -3.8999999999999995 9.5 3141.8 nuORFdb_human ENST00000372714.5_1_10:76586522-76602417:+ ENST00000372714.5_1_10:76586522-76602417:+|ENST00000372724.5_1_10:76598507-76602417:+ KAT6B 5' uORF 5' uORF GN=KAT6B 5' uORF E_20221201_NO30_400nL_HLAc1_1e7_directIP_titration_rep3_Slot2-3_1_3514 20mio-rep2 E_20221201_NO30_400nL_HLAc1_2e7_directIP_titration_rep3_Slot2-4_1_3518.15240.1348.2 2 4 1 12.91 5.8 1.67 7.11 72.2 6 1 1 27500.0 M15m M20m Hydrogen 15 (K) 9 mFFTRmQSF (L) 9 PRCDLGmQSF m F|F|T\R m\Q|S\F 55.6 5 6 3 3 0 dual b/y bias m:Oxidized methionine 52.639706 0.908792 613.7689 1194.543 31.9873 -2.1 4.199999999999999 9.5 3141.8 nuORFdb_human ENST00000372714.5_1_10:76586522-76602417:+ ENST00000372714.5_1_10:76586522-76602417:+|ENST00000372724.5_1_10:76598507-76602417:+ KAT6B 5' uORF 5' uORF GN=KAT6B 5' uORF E_20221201_NO30_400nL_HLAc1_2e7_directIP_titration_rep3_Slot2-4_1_3518 20mio-rep3 E_20221201_NO30_400nL_HLAc1_2e7_directIP_titration_rep4_Slot2-4_1_3519.15249.1359.2 2 4 1 13.79 3.33 1.21 10.46 70.8 6 1 1 31300.0 M15m M20m Hydrogen 15 (K) 9 mFFTRmQSF (L) 9 FmESGHAFPST m F|F|T|R/m Q|S\F 55.6 5 5 5 4 0 dual b/y bias m:Oxidized methionine 52.672382 0.901741 613.766 1194.543 31.9813 -7.0 -1.2000000000000002 9.5 3141.8 nuORFdb_human ENST00000372714.5_1_10:76586522-76602417:+ ENST00000372714.5_1_10:76586522-76602417:+|ENST00000372724.5_1_10:76598507-76602417:+ KAT6B 5' uORF 5' uORF GN=KAT6B 5' uORF E_20221201_NO30_400nL_HLAc1_2e7_directIP_titration_rep4_Slot2-4_1_3519 40mio-rep1 E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep1_Slot1-04_1_3496.15288.1315.2 2 3 1 16.24 8.24 2.13 8.0 85.0 7 1 1 53400.0 M15m M20m Hydrogen 15 (K) 9 mFFTRmQSF (L) 9 EVmEQLNNSF m F|F|T/R/m\Q|S\F 77.8 7 5 5 3 0 dual b/y bias m:Oxidized methionine 52.808723 0.929759 613.7691 1194.543 31.9873 -2.1 1.1 9.5 3141.8 nuORFdb_human ENST00000372714.5_1_10:76586522-76602417:+ ENST00000372714.5_1_10:76586522-76602417:+|ENST00000372724.5_1_10:76598507-76602417:+ KAT6B 5' uORF 5' uORF GN=KAT6B 5' uORF E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep1_Slot1-04_1_3496 40mio-rep2 E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep2_Slot2-5_1_3520.15105.1341.2 2 5 1 15.09 7.17 3.46 7.92 79.8 7 1 1 58000.0 M15m M20m Hydrogen 15 (K) 9 mFFTRmQSF (L) 9 PRCDLGmQSF m F/F|T|R|m\Q|S\F 77.8 7 6 5 4 0 dual b/y bias m:Oxidized methionine 52.179066 0.913444 613.7674 1194.543 31.9833 -5.3 -0.39999999999999947 9.5 3141.8 nuORFdb_human ENST00000372714.5_1_10:76586522-76602417:+ ENST00000372714.5_1_10:76586522-76602417:+|ENST00000372724.5_1_10:76598507-76602417:+ KAT6B 5' uORF 5' uORF GN=KAT6B 5' uORF E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep2_Slot2-5_1_3520 40mio-rep3 E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep3_Slot2-5_1_3521.15000.1340.2 2 4 1 15.26 4.34 3.43 10.91 80.2 7 1 1 41200.0 M15m M20m Hydrogen 15 (K) 9 mFFTRmQSF (L) 9 YMmAKAPHDF m F|F|T|R|m\Q\S\F 77.8 7 7 4 4 0 dual b/y bias m:Oxidized methionine 51.818005 0.914091 613.7691 1194.543 31.9873 -2.1 2.4999999999999996 9.5 3141.8 nuORFdb_human ENST00000372714.5_1_10:76586522-76602417:+ ENST00000372714.5_1_10:76586522-76602417:+|ENST00000372724.5_1_10:76598507-76602417:+ KAT6B 5' uORF 5' uORF GN=KAT6B 5' uORF E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep3_Slot2-5_1_3521 40mio-rep4 E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep4_Slot2-5_1_3522.14992.1337.2 2 6 1 14.91 4.53 3.36 10.38 75.5 8 1 1 48100.0 M15m M20m Hydrogen 15 (K) 9 mFFTRmQSF (L) 9 FmmELTAHQS m/F|F|T|R\m\Q\S\F 100.0 9 7 4 3 0 dual b/y bias m:Oxidized methionine 51.775936 0.915531 613.7667 1194.543 31.9833 -5.3 -0.5999999999999996 9.5 3141.8 nuORFdb_human ENST00000372714.5_1_10:76586522-76602417:+ ENST00000372714.5_1_10:76586522-76602417:+|ENST00000372724.5_1_10:76598507-76602417:+ KAT6B 5' uORF 5' uORF GN=KAT6B 5' uORF E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep4_Slot2-5_1_3522 5mio-rep1 E_20221201_NO30_400nL_HLAc1_5e6_directIP_titration_rep1_Slot1-37_1_3502.15406.1350.2 2 2 1 10.19 2.29 10.19 60.5 6 1 1 7830.0 M15m M20m Hydrogen 15 (K) 9 mFFTRmQSF (L) 9 m F|F/T/R|m-Q|S\F 77.8 7 4 5 3 1 dual b/y bias m:Oxidized methionine 53.218151 0.907836 613.7681 1194.543 31.9853 -3.7 3.3 9.5 3141.8 nuORFdb_human ENST00000372714.5_1_10:76586522-76602417:+ ENST00000372714.5_1_10:76586522-76602417:+|ENST00000372724.5_1_10:76598507-76602417:+ KAT6B 5' uORF 5' uORF GN=KAT6B 5' uORF E_20221201_NO30_400nL_HLAc1_5e6_directIP_titration_rep1_Slot1-37_1_3502 SCP E_20221201_NO30_400nL_HLAc1_4e7_PDAC_Slot2-6_1_3524.25369.418.1 1 2 1 10.2 4.86 6.01 5.33 74.8 6 0 0 19400.0 Hydrogen 45 (L) 9 MIVDGVITM (A) 9 LIPSMSVTM M I\V D\G\V\I\T\M 55.6 5 6 0 0 0 b-ion bias 87.642052 1.474643 978.5002 978.5 0.0001 0.2 2.6 3.8 9612.2 nuORFdb_human ENST00000441189.3_1_X:41200742-41206988:+ ENST00000441189.3_1_X:41200742-41206988:+|ENST00000399959.6_1_X:41200742-41202586:+ DDX3X 3' dORF 3' dORF GN=DDX3X 3' dORF E_20221201_NO30_400nL_HLAc1_4e7_PDAC_Slot2-6_1_3524 40mio-rep1 E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep1_Slot1-04_1_3496.21787.475.1 1 1 1 9.65 6.9 3.29 2.75 78.8 5 0 0 5470.0 Hydrogen 54 (S) 9 MLASQVPPL (S) 9 AQFPSVPIP M L A S\Q|V|P\P-L 55.6 5 4 2 2 1 b-ion bias 75.263161 1.440735 955.5244 955.528 -0.0041 -4.3 -1.0999999999999996 5.27 10441.8 nuORFdb_human ENST00000547701.5_1_12:58220852-58239300:- ENST00000547701.5_1_12:58220852-58239300:-|ENST00000550144.1_1_12:58221318-58223298:-|ENST00000551594.1_1_12:58220812-58223346:- CTDSP2 5' Overlap uORF 5' Overlap uORF GN=CTDSP2 5' Overlap uORF E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep1_Slot1-04_1_3496 SCP E_20221201_NO30_400nL_HLAc1_4e7_PDAC_Slot2-6_1_3524.25807.1281.2 2 2 1 17.28 8.69 9.23 8.59 85.2 9 1 1 6740.0 Hydrogen 59 (R) 11 MLLSHTGKLAL (-) 11 MLLALQRPEL M/L/L|S/H/T|G K\L\A\L 81.8 9 5 6 2 0 unbiased 89.155418 0.950855 592.3474 1183.687 -0.0 -0.0 2.4 8.52 7681.0 nuORFdb_human ENST00000500112.1_1_8:128221640-128221847:- ENST00000500112.1_1_8:128221640-128221847:- CCAT1 lincRNA lncRNA GN=CCAT1 lincRNA E_20221201_NO30_400nL_HLAc1_4e7_PDAC_Slot2-6_1_3524 SCP E_20221201_NO30_400nL_HLAc1_4e7_PDAC_Slot2-6_1_3524.14090.1265.2 2 1 1 8.64 1.76 4.28 6.88 58.4 6 1 1 9600.0 M59m Hydrogen 59 (R) 11 mLLSHTGKLAL (-) 11 YmILSLFIAL m L|L|S|H T G K\L\A\L 45.5 5 6 3 3 0 b-ion bias m:Oxidized methionine 48.676617 0.960742 600.3433 1183.687 15.992 -2.4 0.0 8.52 7681.0 nuORFdb_human ENST00000500112.1_1_8:128221640-128221847:- ENST00000500112.1_1_8:128221640-128221847:- CCAT1 lincRNA lncRNA GN=CCAT1 lincRNA E_20221201_NO30_400nL_HLAc1_4e7_PDAC_Slot2-6_1_3524 40mio-rep1 E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep1_Slot1-04_1_3496.18118.428.1 1 2 1 8.95 3.48 8.95 52.2 7 0 0 4020.0 M36m Hydrogen 28 (K) 9 MLSGFTPTm (S) 9 M L/S/G/F|T|P/T\m 77.8 7 3 6 2 0 y-ion bias m:Oxidized methionine 62.58643 1.468745 1000.4414 984.453 15.9871 -7.8 -4.6 5.27 9358.6 nuORFdb_human ENST00000540982.1_1_12:9550954-9555054:- ENST00000540982.1_1_12:9550954-9555054:-|ENST00000520314.2_2_12:9724113-9727568:+ RP11-599J14.2 Pseudogene Pseudogene GN=RP11-599J14.2 Pseudogene E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep1_Slot1-04_1_3496 Exploris_FAIMS VH20221207_MKP_FAIMS2022E_HLAc1_4e7_PDAC.59585.59736.2 2 1 1 11.75 6.2 8.1 5.55 85.4 6 1 1 9730000.0 Hydrogen 10 (E) 11 MPAFLSRASAL (T) 11 MPAGKQKLQY M/P|A|F/L/S R A S A/L 54.5 6 2 6 2 0 y-ion bias 0.997 89.7 103.53 39.04 -70.0 582.3126 1163.624 -0.0061 -5.3 -2.6999999999999997 9.5 2761.2 nuORFdb_human ENST00000603524.5_1_19:35610107-35610293:+ ENST00000603524.5_1_19:35610107-35610293:+ FXYD3 5' Overlap uORF 5' Overlap uORF GN=FXYD3 5' Overlap uORF VH20221207_MKP_FAIMS2022E_HLAc1_4e7_PDAC SCP E_20221201_NO30_400nL_HLAc1_4e7_PDAC_Slot2-6_1_3524.12324.1255.2 2 1 1 10.71 4.06 6.27 6.64 79.4 7 0 0 34300.0 Hydrogen 45 (L) 10 MPCQHQLNCF (F) 10 MSnSmINTLCF M/P C\Q H\Q\L|N|C\F 60.0 6 6 3 2 0 b-ion bias Carbamidomethylation C:Carbamidomethylation, 42.575447 0.966913 667.7741 1334.544 -0.0031 -2.3 0.10000000000000009 6.49 7136.0 nuORFdb_human ENST00000297598.4_1_8:94929214-94934336:+ ENST00000297598.4_1_8:94929214-94934336:+|ENST00000520728.5_1_8:94929177-94934336:+|ENST00000518573.1_1_8:94929413-94934336:+|ENST00000517764.1_1_8:94930131-94934336:+|ENST00000521144.1_1_8:94932150-94934336:+|ENST00000518827.1_1_8:94934052-94934336:+ PDP1 5' Overlap uORF 5' Overlap uORF GN=PDP1 5' Overlap uORF E_20221201_NO30_400nL_HLAc1_4e7_PDAC_Slot2-6_1_3524 Exploris_FAIMS VH20221207_MKP_FAIMS2022E_HLAc1_4e7_PDAC.55031.55150.2 2 1 1 11.93 4.18 7.6 7.75 86.9 7 0 0 4400000.0 Hydrogen 15 (V) 10 MPENPVSVTF (L) 10 PSSHSATSVTF M P E-N\P\V\S\V/T/F 70.0 7 4 2 0 1 b-ion bias 0.92 100.0 96.5 120.0 -70.0 560.7695 1120.534 -0.0025 -2.3 0.30000000000000027 4.0 4960.6 nuORFdb_human ENST00000256194.8_2_11:12159950-12183696:+ ENST00000256194.8_2_11:12159950-12183696:+ MICAL2 5' uORF 5' uORF GN=MICAL2 5' uORF VH20221207_MKP_FAIMS2022E_HLAc1_4e7_PDAC SCP E_20221201_NO30_400nL_HLAc1_4e7_PDAC_Slot2-6_1_3524.27007.1342.2 2 2 1 16.85 7.43 9.73 9.41 85.4 8 0 0 68800.0 Hydrogen 1 (-) 9 MPFLHLHNM (S) 9 AYLMIFHNM M/P/F|L/H\L|H|N\M 100.0 9 5 6 3 0 dual b/y bias 93.302254 0.912855 570.2766 1139.549 -0.0021 -1.9 0.5 6.69 3266.8 smORF_v4|nuORFdb_human PCSK7_11:117101066-117100572:- PCSK7_11:117101066-117100572:-|ENST00000532301.5_1_11:117100562-117101901:-|ENST00000524507.5_1_11:117100562-117102552:-|ENST00000320934.7_1_11:117100562-117103224:- PCSK7 uORF 11:117101066-117100572:- 11 PCSK7 5' uORF 5' uORF GN=PCSK7 E_20221201_NO30_400nL_HLAc1_4e7_PDAC_Slot2-6_1_3524 SCP E_20221201_NO30_400nL_HLAc1_4e7_PDAC_Slot2-6_1_3524.18068.1442.2 2 1 1 8.39 8.39 8.39 54.6 6 0 1 11000.0 Hydrogen 81 (N) 9 MPFQDGKPF (N) 9 M/P/F/Q/D|G K|P F 55.6 5 2 6 2 0 y-ion bias 62.419395 0.850002 533.7521 1066.503 -0.0059 -5.5 -3.1 5.59 17668.9 nuORFdb_human ENST00000412609.5_1_19:40170059-40174276:+ ENST00000412609.5_1_19:40170059-40174276:+|ENST00000598164.2_1_19:40172096-40174784:+ LGALS17A Pseudogene Pseudogene GN=LGALS17A Pseudogene E_20221201_NO30_400nL_HLAc1_4e7_PDAC_Slot2-6_1_3524 Exploris_FAIMS VH20221207_MKP_FAIMS2022E_HLAc1_4e7_PDAC.45051.45051.2 2 1 1 14.0 3.22 14.0 10.77 84.5 7 0 1 12600000.0 Hydrogen 81 (N) 9 MPFQDGKPF (N) 9 FQGDKMPPF M/P|F|Q/D-G K/P/F 77.8 7 2 6 2 1 y-ion bias 0.902 57.3 81.5 47.23 -70.0 533.7542 1066.503 -0.0016 -1.5 1.1 5.59 17668.9 nuORFdb_human ENST00000412609.5_1_19:40170059-40174276:+ ENST00000412609.5_1_19:40170059-40174276:+|ENST00000598164.2_1_19:40172096-40174784:+ LGALS17A Pseudogene Pseudogene GN=LGALS17A Pseudogene VH20221207_MKP_FAIMS2022E_HLAc1_4e7_PDAC Exploris_FAIMS VH20221207_MKP_FAIMS2022E_HLAc1_4e7_PDAC.36129.36129.2 2 1 1 7.34 7.34 7.34 53.6 6 0 1 2780000.0 M81m Hydrogen 81 (N) 9 mPFQDGKPF (N) 9 m/P\F\Q/D-G K/P-F 77.8 7 2 3 0 2 unbiased m:Oxidized methionine 0.988 98.4 67.78 110.11 -70.0 541.7512 1066.503 15.9925 -2.3 0.30000000000000027 5.59 17668.9 nuORFdb_human ENST00000412609.5_1_19:40170059-40174276:+ ENST00000412609.5_1_19:40170059-40174276:+|ENST00000598164.2_1_19:40172096-40174784:+ LGALS17A Pseudogene Pseudogene GN=LGALS17A Pseudogene VH20221207_MKP_FAIMS2022E_HLAc1_4e7_PDAC SCP E_20221201_NO30_400nL_HLAc1_4e7_PDAC_Slot2-6_1_3524.25643.1274.2 2 4 1 17.99 7.48 12.21 10.52 86.6 8 0 1 114000.0 Hydrogen 81 (N) 11 MPFQDGKPFNL (C) 11 MAKAAASSSLEDL M/P/F|Q|D|G K|P F\N\L 63.6 7 6 6 4 0 dual b/y bias 88.588127 0.954763 647.3175 1293.63 -0.0009 -0.7 1.7 5.59 17668.9 nuORFdb_human ENST00000412609.5_1_19:40170059-40174276:+ ENST00000412609.5_1_19:40170059-40174276:+|ENST00000598164.2_1_19:40172096-40174784:+ LGALS17A Pseudogene Pseudogene GN=LGALS17A Pseudogene E_20221201_NO30_400nL_HLAc1_4e7_PDAC_Slot2-6_1_3524 Exploris_FAIMS VH20221207_MKP_FAIMS2022E_HLAc1_4e7_PDAC.58625.58764.2 2 1 1 9.55 9.02 9.55 66.7 7 0 1 21900000.0 Hydrogen 81 (N) 11 MPFQDGKPFNL (C) 11 M/P/F\Q/D/G K|P F/N L 45.5 5 2 6 1 0 y-ion bias 0.818 91.9 102.73 120.0 -50.0 647.3171 1293.63 -0.0027 -2.1 0.5 5.59 17668.9 nuORFdb_human ENST00000412609.5_1_19:40170059-40174276:+ ENST00000412609.5_1_19:40170059-40174276:+|ENST00000598164.2_1_19:40172096-40174784:+ LGALS17A Pseudogene Pseudogene GN=LGALS17A Pseudogene VH20221207_MKP_FAIMS2022E_HLAc1_4e7_PDAC Eva-50mio-3fr AL20210827_EV_HLA_PDAC_PANFRO413_2D_50e6_W632_classI_fxn3.31986.31986.2 2 2 2 12.22 9.51 12.22 72.0 8 0 1 8240000.0 M81m Hydrogen 81 (N) 11 mPFQDGKPFNL (C) 11 m/P|F|Q/D/G K/P F/N-L 63.6 7 2 7 2 1 y-ion bias m:Oxidized methionine 0.904 100.0 58.17 100.0 0.0 655.3145 1293.63 15.9921 -2.1 -2.6 5.59 17668.9 nuORFdb_human ENST00000412609.5_1_19:40170059-40174276:+ ENST00000412609.5_1_19:40170059-40174276:+|ENST00000598164.2_1_19:40172096-40174784:+ LGALS17A Pseudogene Pseudogene GN=LGALS17A Pseudogene AL20210827_EV_HLA_PDAC_PANFRO413_2D_50e6_W632_classI_fxn3 Eva-50mio-3fr AL20210827_EV_HLA_PDAC_PANFRO413_2D_50e6_W632_classI_fxn3.34625.34727.2 2 2 2 14.09 9.43 9.24 4.65 88.7 6 0 0 540000000.0 Hydrogen 35 (S) 8 MPFVHVVY (A) 8 VHFPMVVY M/P F-V/H\V|V/Y 75.0 6 2 4 1 1 y-ion bias 0.987 94.9 63.42 21.3 0.0 496.2572 991.507 0.0002 0.2 -0.3 6.49 4834.4 nuORFdb_human ENST00000602122.5_1_19:35739636-35739768:+ ENST00000602122.5_1_19:35739636-35739768:+ LSR 5' uORF 5' uORF GN=LSR 5' uORF AL20210827_EV_HLA_PDAC_PANFRO413_2D_50e6_W632_classI_fxn3 Exploris AL20221201_MKP_PDAC_01.46123.47013.2 2 6 1 16.37 10.58 10.25 5.79 92.3 6 0 0 13300000000.0 Hydrogen 35 (S) 8 MPFVHVVY (A) 8 VHFPMVVY M/P F-V/H\V|V/Y 75.0 6 2 4 1 1 y-ion bias 0.985 100.0 71.78 31.4 0.0 496.2573 991.507 0.0004 0.4 -0.4 6.49 4834.4 nuORFdb_human ENST00000602122.5_1_19:35739636-35739768:+ ENST00000602122.5_1_19:35739636-35739768:+ LSR 5' uORF 5' uORF GN=LSR 5' uORF AL20221201_MKP_PDAC_01 SCP E_20221201_NO30_400nL_HLAc1_4e7_PDAC_Slot2-6_1_3524.23971.1465.2 2 47 1 19.8 8.84 13.26 10.97 98.8 7 0 0 2260000.0 Hydrogen 35 (S) 8 MPFVHVVY (A) 8 VHFPMVVY M/P/F|V/H|V|V\Y 100.0 8 4 6 3 0 dual b/y bias 82.813255 0.835656 496.2564 991.507 -0.0023 -2.3 0.10000000000000009 6.49 4834.4 nuORFdb_human ENST00000602122.5_1_19:35739636-35739768:+ ENST00000602122.5_1_19:35739636-35739768:+ LSR 5' uORF 5' uORF GN=LSR 5' uORF E_20221201_NO30_400nL_HLAc1_4e7_PDAC_Slot2-6_1_3524 Exploris_FAIMS VH20221207_MKP_FAIMS2022E_HLAc1_4e7_PDAC.53717.54576.2 2 4 1 15.28 11.73 8.45 3.56 89.5 5 0 0 2200000000.0 Hydrogen 35 (S) 8 MPFVHVVY (A) 8 VHFPMVVY M P F\V/H\V|V/Y 62.5 5 3 3 1 0 unbiased 0.979 82.6 95.27 22.99 -50.0 496.2559 991.507 -0.0024 -2.5 0.10000000000000009 6.49 4834.4 nuORFdb_human ENST00000602122.5_1_19:35739636-35739768:+ ENST00000602122.5_1_19:35739636-35739768:+ LSR 5' uORF 5' uORF GN=LSR 5' uORF VH20221207_MKP_FAIMS2022E_HLAc1_4e7_PDAC Eva-50mio-3fr AL20210827_EV_HLA_PDAC_PANFRO413_2D_50e6_W632_classI_fxn3.27896.27996.2 2 1 1 11.39 10.09 5.47 1.31 63.9 5 0 0 583000000.0 M35m Hydrogen 35 (S) 8 mPFVHVVY (A) 8 VHFPmVVY m P\F V\H\V|V/Y 50.0 4 4 2 1 0 b-ion bias m:Oxidized methionine 0.991 100.0 51.5 1.64 0.0 504.2543 991.507 15.9944 -0.5 -1.0 6.49 4834.4 nuORFdb_human ENST00000602122.5_1_19:35739636-35739768:+ ENST00000602122.5_1_19:35739636-35739768:+ LSR 5' uORF 5' uORF GN=LSR 5' uORF AL20210827_EV_HLA_PDAC_PANFRO413_2D_50e6_W632_classI_fxn3 SCP E_20221201_NO30_400nL_HLAc1_4e7_PDAC_Slot2-6_1_3524.16524.1472.2 2 11 1 17.48 11.52 7.85 5.95 88.6 7 0 0 375000.0 M35m Hydrogen 35 (S) 8 mPFVHVVY (A) 8 VHFPmVVY m/P/F/V|H\V|V\Y 100.0 8 4 5 2 0 unbiased m:Oxidized methionine 57.085709 0.830967 504.2532 991.507 15.9917 -3.2 -0.8000000000000003 6.49 4834.4 nuORFdb_human ENST00000602122.5_1_19:35739636-35739768:+ ENST00000602122.5_1_19:35739636-35739768:+ LSR 5' uORF 5' uORF GN=LSR 5' uORF E_20221201_NO30_400nL_HLAc1_4e7_PDAC_Slot2-6_1_3524 Eva-50mio-3fr AL20210827_EV_HLA_PDAC_PANFRO413_2D_50e6_W632_classI_fxn3.27255.27255.3 3 1 1 9.29 0.22 9.29 9.07 74.3 6 0 0 2660000.0 Hydrogen 35 (S) 10 MPFVHVVYAH (-) 10 PSAFPRSSTLH M P F-V/H/V/V-Y/A/H 70.0 7 0 5 0 2 y-ion bias 0.914 76.9 50.4 100.0 0.0 400.5398 1199.603 0.0018 1.5 1.0 6.69 4834.4 nuORFdb_human ENST00000602122.5_1_19:35739636-35739768:+ ENST00000602122.5_1_19:35739636-35739768:+ LSR 5' uORF 5' uORF GN=LSR 5' uORF AL20210827_EV_HLA_PDAC_PANFRO413_2D_50e6_W632_classI_fxn3 SCP E_20221201_NO30_400nL_HLAc1_4e7_PDAC_Slot2-6_1_3524.12288.1550.3 3 1 1 11.52 3.33 7.48 8.2 61.8 7 0 0 5470.0 M35m Hydrogen 35 (S) 10 mPFVHVVYAH (-) 10 KVNFLNNGYm m/P/F/V/H|V|V Y A-H 70.0 7 2 6 2 1 y-ion bias m:Oxidized methionine 42.45116 0.782104 405.8678 1199.603 15.9864 -7.0 -4.6 6.69 4834.4 nuORFdb_human ENST00000602122.5_1_19:35739636-35739768:+ ENST00000602122.5_1_19:35739636-35739768:+ LSR 5' uORF 5' uORF GN=LSR 5' uORF E_20221201_NO30_400nL_HLAc1_4e7_PDAC_Slot2-6_1_3524 SCP E_20221201_NO30_400nL_HLAc1_4e7_PDAC_Slot2-6_1_3524.13664.473.1 1 7 1 13.55 3.66 9.82 9.88 83.2 8 0 0 164000.0 Hydrogen 27 (P) 9 MPGNSSLDY (C) 9 MSKDQPDY M/P/G/N\S|S\L\D\Y 100.0 9 5 4 1 0 unbiased 47.205341 1.442114 983.4118 983.414 -0.0019 -1.9 0.5 3.8 4571.0 nuORFdb_human ENST00000346329.7_1_11:70244658-70251434:+ ENST00000346329.7_1_11:70244658-70251434:+ CTTN 5' uORF 5' uORF GN=CTTN 5' uORF E_20221201_NO30_400nL_HLAc1_4e7_PDAC_Slot2-6_1_3524 Exploris_FAIMS VH20221207_MKP_FAIMS2022E_HLAc1_4e7_PDAC.27398.27398.2 2 2 1 8.84 3.74 4.32 5.11 53.8 6 0 0 10400000.0 M27m Hydrogen 27 (P) 9 mPGNSSLDY (C) 9 GGMGDATLDY m P\G N\S\S\L|D/Y 55.6 5 5 2 1 0 b-ion bias m:Oxidized methionine 0.984 97.8 55.02 120.0 -70.0 500.2066 983.414 15.992 -2.9 -0.2999999999999998 3.8 4571.0 nuORFdb_human ENST00000346329.7_1_11:70244658-70251434:+ ENST00000346329.7_1_11:70244658-70251434:+ CTTN 5' uORF 5' uORF GN=CTTN 5' uORF VH20221207_MKP_FAIMS2022E_HLAc1_4e7_PDAC Exploris AL20221201_MKP_PDAC_01.31792.31792.2 2 2 1 12.61 4.32 10.75 8.29 67.3 6 1 1 3690000000.0 Hydrogen 90 (R) 9 MPGRLICTY (-) 9 MPQQPPLHY M/P/G R\L I\C|T|Y 55.6 5 4 4 2 0 unbiased Carbamidomethylation C:Carbamidomethylation, 0.874 89.6 50.65 0.79 0.0 555.7757 1110.543 0.0007 0.7 -0.10000000000000009 8.5 10472.2 nuORFdb_human ENST00000558939.5_1_15:70389852-70390146:- ENST00000558939.5_1_15:70389852-70390146:- TLE3 5' uORF 5' uORF GN=TLE3 5' uORF AL20221201_MKP_PDAC_01 SCP E_20221201_NO30_400nL_HLAc1_4e7_PDAC_Slot2-6_1_3524.14335.1402.2 2 6 1 14.69 4.63 8.91 10.06 87.9 6 1 1 607000.0 Hydrogen 90 (R) 9 MPGRLICTY (-) 9 MIDNLKVDY M/P/G R L-I|C|T\Y 66.7 6 3 4 2 1 unbiased Carbamidomethylation C:Carbamidomethylation, 49.522495 0.875096 555.7733 1110.543 -0.0047 -4.3 -1.9 8.5 10472.2 nuORFdb_human ENST00000558939.5_1_15:70389852-70390146:- ENST00000558939.5_1_15:70389852-70390146:- TLE3 5' uORF 5' uORF GN=TLE3 5' uORF E_20221201_NO30_400nL_HLAc1_4e7_PDAC_Slot2-6_1_3524 Exploris_FAIMS VH20221207_MKP_FAIMS2022E_HLAc1_4e7_PDAC.38046.38046.2 2 2 1 12.05 12.05 12.05 65.4 6 1 1 1300000000.0 Hydrogen 90 (R) 9 MPGRLICTY (-) 9 M/P/G R\L I\C|T/Y 55.6 5 3 4 1 0 unbiased Carbamidomethylation C:Carbamidomethylation, 0.974 68.1 70.47 6.01 -50.0 555.7734 1110.543 -0.0038 -3.5 -0.8999999999999999 8.5 10472.2 nuORFdb_human ENST00000558939.5_1_15:70389852-70390146:- ENST00000558939.5_1_15:70389852-70390146:- TLE3 5' uORF 5' uORF GN=TLE3 5' uORF VH20221207_MKP_FAIMS2022E_HLAc1_4e7_PDAC Exploris AL20221201_MKP_PDAC_01.25281.25416.2 2 2 1 11.9 5.68 11.9 6.22 68.4 6 1 1 1170000000.0 M90m Hydrogen 90 (R) 9 mPGRLICTY (-) 9 LVWVWCTY m/P/G R L\I\C|T|Y 66.7 6 4 4 2 0 unbiased Carbamidomethylation C:Carbamidomethylation,m:Oxidized methionine 0.98 83.8 41.82 3.08 0.0 563.7721 1110.543 15.9934 -1.3 -2.1 8.5 10472.2 nuORFdb_human ENST00000558939.5_1_15:70389852-70390146:- ENST00000558939.5_1_15:70389852-70390146:- TLE3 5' uORF 5' uORF GN=TLE3 5' uORF AL20221201_MKP_PDAC_01 SCP E_20221201_NO30_400nL_HLAc1_4e7_PDAC_Slot2-6_1_3524.11310.1384.2 2 4 1 17.04 7.17 10.77 9.87 83.2 8 1 1 283000.0 M90m Hydrogen 90 (R) 9 mPGRLICTY (-) 9 LVWVWCTY m/P/G/R\L\I|C|T\Y 100.0 9 5 5 2 0 unbiased Carbamidomethylation C:Carbamidomethylation,m:Oxidized methionine 39.073952 0.886509 563.7727 1110.543 15.9953 0.3 2.6999999999999997 8.5 10472.2 nuORFdb_human ENST00000558939.5_1_15:70389852-70390146:- ENST00000558939.5_1_15:70389852-70390146:- TLE3 5' uORF 5' uORF GN=TLE3 5' uORF E_20221201_NO30_400nL_HLAc1_4e7_PDAC_Slot2-6_1_3524 Exploris_FAIMS VH20221207_MKP_FAIMS2022E_HLAc1_4e7_PDAC.31084.31084.2 2 2 1 12.29 3.12 12.29 9.17 68.3 7 1 1 848000000.0 M90m Hydrogen 90 (R) 9 mPGRLICTY (-) 9 LVWVWCTY m/P/G R\L\I\C|T/Y 77.8 7 4 4 1 0 unbiased Carbamidomethylation C:Carbamidomethylation,m:Oxidized methionine 0.987 72.7 60.13 7.45 -50.0 563.7715 1110.543 15.9924 -2.3 0.30000000000000027 8.5 10472.2 nuORFdb_human ENST00000558939.5_1_15:70389852-70390146:- ENST00000558939.5_1_15:70389852-70390146:- TLE3 5' uORF 5' uORF GN=TLE3 5' uORF VH20221207_MKP_FAIMS2022E_HLAc1_4e7_PDAC Exploris AL20221201_MKP_PDAC_01.17131.17131.2 2 1 1 15.69 5.1 10.95 10.6 84.3 8 0 0 5020000.0 Hydrogen 23 (L) 11 MPHEADTSCPY (-) 11 CmDnRTFSYP M P H\E\A\D\T\S|C|P/Y 72.7 8 7 3 2 0 b-ion bias Carbamidomethylation C:Carbamidomethylation, 0.526 87.7 30.32 62.72 0.0 654.2566 1307.503 0.0028 2.1 1.3 4.35 3931.5 nuORFdb_human ENST00000501396.5_1_8:128302103-128302205:- ENST00000501396.5_1_8:128302103-128302205:- CASC8 Antisense lncRNA GN=CASC8 Antisense AL20221201_MKP_PDAC_01 SCP E_20221201_NO30_400nL_HLAc1_4e7_PDAC_Slot2-6_1_3524.8542.1308.2 2 2 1 14.22 7.38 9.27 6.83 81.8 8 0 0 66600.0 Hydrogen 23 (L) 11 MPHEADTSCPY (-) 11 SLFAcnICPY M P H\E\A/D\T|S|C|P\Y 72.7 8 7 4 3 0 unbiased Carbamidomethylation C:Carbamidomethylation, 29.510778 0.934129 654.2533 1307.503 -0.0044 -3.3 -0.8999999999999999 4.35 3931.5 nuORFdb_human ENST00000501396.5_1_8:128302103-128302205:- ENST00000501396.5_1_8:128302103-128302205:- CASC8 Antisense lncRNA GN=CASC8 Antisense E_20221201_NO30_400nL_HLAc1_4e7_PDAC_Slot2-6_1_3524 SCP E_20221201_NO30_400nL_HLAc1_4e7_PDAC_Slot2-6_1_3524.5840.1312.2 2 1 1 10.17 5.26 3.85 4.91 78.3 6 0 0 4250.0 M23m Hydrogen 23 (L) 11 mPHEADTSCPY (-) 11 CVcPSGGHQPY m P H E A\D/T\S|C|P\Y 54.5 6 5 3 2 0 unbiased Carbamidomethylation C:Carbamidomethylation,m:Oxidized methionine 20.182426 0.931484 662.2516 1307.503 15.9936 -1.0 1.4 4.35 3931.5 nuORFdb_human ENST00000501396.5_1_8:128302103-128302205:- ENST00000501396.5_1_8:128302103-128302205:- CASC8 Antisense lncRNA GN=CASC8 Antisense E_20221201_NO30_400nL_HLAc1_4e7_PDAC_Slot2-6_1_3524 Exploris_FAIMS VH20221207_MKP_FAIMS2022E_HLAc1_4e7_PDAC.44975.44975.2 2 1 1 9.89 9.89 9.89 68.3 6 0 0 5510000.0 Hydrogen 9 (T) 8 MPIAVQCY (-) 8 M P\I\A\V\Q|C/Y 75.0 6 5 2 1 0 b-ion bias Carbamidomethylation C:Carbamidomethylation, 0.937 81.8 80.95 120.0 -70.0 491.2285 981.453 -0.0035 -3.6 -1.0 5.27 1810.0 nuORFdb_human ENST00000544404.5_1_1:220246204-220246252:- ENST00000544404.5_1_1:220246204-220246252:- BPNT1 Out-of-Frame Out-of-Frame GN=BPNT1 Out-of-Frame VH20221207_MKP_FAIMS2022E_HLAc1_4e7_PDAC Exploris_FAIMS VH20221207_MKP_FAIMS2022E_HLAc1_4e7_PDAC.58020.58020.2 2 3 1 15.84 5.96 15.31 9.88 86.3 8 1 1 42400000.0 Hydrogen 1 (-) 9 MPIQVLKGL (T) 9 PMLVAGLKGL M/P|I/Q|V|L/K/G/L 100.0 9 3 8 3 0 dual b/y bias 0.976 69.1 100.97 120.0 -70.0 499.8057 998.607 -0.0025 -2.5 0.10000000000000009 8.5 1450.8 nuORFdb_human ENST00000345523.8_1_19:51302584-51305485:- ENST00000345523.8_1_19:51302584-51305485:- C19orf48 5' uORF 5' uORF GN=C19orf48 5' uORF VH20221207_MKP_FAIMS2022E_HLAc1_4e7_PDAC Eva-50mio-3fr AL20210827_EV_HLA_PDAC_PANFRO413_2D_50e6_W632_classI_fxn3.31717.31717.2 2 2 2 15.03 9.22 15.03 5.82 79.9 8 1 1 16000000.0 M1m Hydrogen 1 (-) 9 mPIQVLKGL (T) 9 PmLVAGLKGL m/P|I|Q/V|L/K/G/L 100.0 9 3 8 3 0 dual b/y bias m:Oxidized methionine 0.983 90.1 58.3 100.0 0.0 507.8052 998.607 15.9965 1.6 1.1 8.5 1450.8 nuORFdb_human ENST00000345523.8_1_19:51302584-51305485:- ENST00000345523.8_1_19:51302584-51305485:- C19orf48 5' uORF 5' uORF GN=C19orf48 5' uORF AL20210827_EV_HLA_PDAC_PANFRO413_2D_50e6_W632_classI_fxn3 Exploris_FAIMS VH20221207_MKP_FAIMS2022E_HLAc1_4e7_PDAC.46933.47072.2 2 1 1 12.31 8.65 12.31 3.66 77.0 7 1 1 18900000.0 M1m Hydrogen 1 (-) 9 mPIQVLKGL (T) 9 PmLVAGLKGL m P\I|Q/V|L/K/G/L 77.8 7 3 6 2 0 y-ion bias m:Oxidized methionine 0.997 92.2 84.2 31.07 -70.0 507.8034 998.607 15.9929 -2.0 0.6000000000000001 8.5 1450.8 nuORFdb_human ENST00000345523.8_1_19:51302584-51305485:- ENST00000345523.8_1_19:51302584-51305485:- C19orf48 5' uORF 5' uORF GN=C19orf48 5' uORF VH20221207_MKP_FAIMS2022E_HLAc1_4e7_PDAC SCP E_20221201_NO30_400nL_HLAc1_4e7_PDAC_Slot2-6_1_3524.5150.1691.3 3 2 1 10.24 4.96 4.28 5.27 85.8 6 2 3 88800.0 Hydrogen 9 (F) 9 MPKKSRISF (S) 9 MDKGKRVLF M/P/K/K/S/R I S\F 66.7 6 1 5 0 0 y-ion bias 17.80018 0.692161 365.2095 1093.619 -0.0032 -2.9 -0.5 11.17 3046.5 nuORFdb_human ENST00000612683.1_1_1:155279859-155279940:+ ENST00000612683.1_1_1:155279859-155279940:+ FDPS Out-of-Frame Out-of-Frame GN=FDPS Out-of-Frame E_20221201_NO30_400nL_HLAc1_4e7_PDAC_Slot2-6_1_3524 Exploris_FAIMS VH20221207_MKP_FAIMS2022E_HLAc1_4e7_PDAC.17722.17722.3 3 1 1 9.01 3.37 4.37 5.64 75.8 6 2 3 5440000.0 Hydrogen 9 (F) 9 MPKKSRISF (S) 9 NNKKIRYSA M/P/K/K/S R I/S/F 66.7 6 0 6 0 0 y-ion bias 0.961 88.8 37.95 90.19 -50.0 365.21 1093.619 -0.0033 -3.0 -0.3999999999999999 11.17 3046.5 nuORFdb_human ENST00000612683.1_1_1:155279859-155279940:+ ENST00000612683.1_1_1:155279859-155279940:+ FDPS Out-of-Frame Out-of-Frame GN=FDPS Out-of-Frame VH20221207_MKP_FAIMS2022E_HLAc1_4e7_PDAC Exploris AL20221201_MKP_PDAC_01.67154.67154.2 2 1 1 12.28 5.5 5.72 6.78 85.8 7 1 1 96700000.0 Hydrogen 1 (-) 10 MPLLENLMRF (S) 10 MPLSTTLVVSSE M/P|L|L/E/N/L M R/F 70.0 7 2 7 2 0 y-ion bias 0.943 93.4 100.43 8.95 0.0 632.334 1263.659 0.002 1.6 0.8 5.75 5035.0 nuORFdb_human ENST00000426421.5_1_7:116528208-116538866:+ ENST00000426421.5_1_7:116528208-116538866:+ CAPZA2 Out-of-Frame Out-of-Frame GN=CAPZA2 Out-of-Frame AL20221201_MKP_PDAC_01 SCP E_20221201_NO30_400nL_HLAc1_4e7_PDAC_Slot2-6_1_3524.29526.1285.2 2 4 1 21.88 7.84 14.22 14.04 97.2 9 1 1 449000.0 Hydrogen 1 (-) 10 MPLLENLMRF (S) 10 MPLSTTLVVSSE M/P|L|L|E|N|L/M|R\F 100.0 10 7 8 6 0 dual b/y bias 102.011185 0.947929 632.3331 1263.659 -0.0001 -0.1 2.3 5.75 5035.0 nuORFdb_human ENST00000426421.5_1_7:116528208-116538866:+ ENST00000426421.5_1_7:116528208-116538866:+ CAPZA2 Out-of-Frame Out-of-Frame GN=CAPZA2 Out-of-Frame E_20221201_NO30_400nL_HLAc1_4e7_PDAC_Slot2-6_1_3524 Exploris_FAIMS VH20221207_MKP_FAIMS2022E_HLAc1_4e7_PDAC.65206.65206.2 2 1 1 13.67 6.45 6.91 7.22 91.7 7 1 1 53400000.0 Hydrogen 1 (-) 10 MPLLENLMRF (S) 10 MPLSTTLVVSSE M/P|L|L/E/N L/M/R F 60.0 6 2 7 2 0 y-ion bias 0.971 97.1 111.17 5.69 -50.0 632.3314 1263.659 -0.0032 -2.6 0.0 5.75 5035.0 nuORFdb_human ENST00000426421.5_1_7:116528208-116538866:+ ENST00000426421.5_1_7:116528208-116538866:+ CAPZA2 Out-of-Frame Out-of-Frame GN=CAPZA2 Out-of-Frame VH20221207_MKP_FAIMS2022E_HLAc1_4e7_PDAC SCP E_20221201_NO30_400nL_HLAc1_4e7_PDAC_Slot2-6_1_3524.28111.1292.2 2 1 1 10.2 4.03 4.98 6.17 68.6 7 1 1 23100.0 M8m Hydrogen 1 (-) 10 MPLLENLmRF (S) 10 MPILSSSGSKMAA M/P/L|L|E|N L/m R\F 60.0 6 4 6 3 0 unbiased m:Oxidized methionine 97.118752 0.943658 640.3294 1263.659 15.9919 -2.3 0.10000000000000009 5.75 5035.0 nuORFdb_human ENST00000426421.5_1_7:116528208-116538866:+ ENST00000426421.5_1_7:116528208-116538866:+ CAPZA2 Out-of-Frame Out-of-Frame GN=CAPZA2 Out-of-Frame E_20221201_NO30_400nL_HLAc1_4e7_PDAC_Slot2-6_1_3524 SCP E_20221201_NO30_400nL_HLAc1_4e7_PDAC_Slot2-6_1_3524.28661.1306.2 2 1 1 8.75 8.75 8.75 58.2 7 1 1 13600.0 M1m Hydrogen 1 (-) 10 mPLLENLMRF (S) 10 m P\L|L|E/N L|M/R\F 60.0 6 5 5 3 0 unbiased m:Oxidized methionine 99.020767 0.934982 640.3291 1263.659 15.9919 -2.3 0.10000000000000009 5.75 5035.0 nuORFdb_human ENST00000426421.5_1_7:116528208-116538866:+ ENST00000426421.5_1_7:116528208-116538866:+ CAPZA2 Out-of-Frame Out-of-Frame GN=CAPZA2 Out-of-Frame E_20221201_NO30_400nL_HLAc1_4e7_PDAC_Slot2-6_1_3524 Exploris_FAIMS VH20221207_MKP_FAIMS2022E_HLAc1_4e7_PDAC.64080.64080.2 2 1 1 11.92 6.95 3.12 4.96 75.4 6 1 1 22600000.0 M1m Hydrogen 1 (-) 10 mPLLENLMRF (S) 10 PmILGYWDIR m/P|L|L/E/N L/M R F 50.0 5 2 6 2 0 y-ion bias m:Oxidized methionine 0.969 100.0 109.5 18.61 -50.0 640.3288 1263.659 15.9915 -2.7 -0.10000000000000009 5.75 5035.0 nuORFdb_human ENST00000426421.5_1_7:116528208-116538866:+ ENST00000426421.5_1_7:116528208-116538866:+ CAPZA2 Out-of-Frame Out-of-Frame GN=CAPZA2 Out-of-Frame VH20221207_MKP_FAIMS2022E_HLAc1_4e7_PDAC Eva-50mio-3fr AL20210827_EV_HLA_PDAC_PANFRO413_2D_50e6_W632_classI_fxn3.37003.37215.2 2 2 2 13.36 4.45 6.52 8.91 90.0 6 1 1 51600000.0 M1m M8m Hydrogen 1 (-) 10 mPLLENLmRF (S) 10 mPLQVPPQIPC m/P|L|L|E/N L/m R F 50.0 5 3 6 3 0 y-ion bias m:Oxidized methionine 0.979 100.0 67.57 100.0 0.0 648.3278 1263.659 31.9895 -0.3 -0.8 5.75 5035.0 nuORFdb_human ENST00000426421.5_1_7:116528208-116538866:+ ENST00000426421.5_1_7:116528208-116538866:+ CAPZA2 Out-of-Frame Out-of-Frame GN=CAPZA2 Out-of-Frame AL20210827_EV_HLA_PDAC_PANFRO413_2D_50e6_W632_classI_fxn3 SCP E_20221201_NO30_400nL_HLAc1_4e7_PDAC_Slot2-6_1_3524.29792.493.1 1 2 1 12.34 9.33 8.23 3.01 89.9 6 0 0 18900.0 Hydrogen 52 (W) 8 MPLLSLAF (L) 8 MLPLTVAF M/P L|L\S\L\A\F 75.0 6 5 2 1 0 b-ion bias 102.929672 1.430084 891.4984 891.501 -0.0028 -3.2 -0.8000000000000003 5.27 8763.2 nuORFdb_human ENST00000555408.5_1_12:56361645-56362592:+ ENST00000555408.5_1_12:56361645-56362592:+ CDK2 3' dORF 3' dORF GN=CDK2 3' dORF E_20221201_NO30_400nL_HLAc1_4e7_PDAC_Slot2-6_1_3524 SCP E_20221201_NO30_400nL_HLAc1_4e7_PDAC_Slot2-6_1_3524.12401.506.1 1 1 1 7.32 -1.94 10.16 9.26 65.1 8 0 0 11300.0 Hydrogen 1 (-) 9 MPNANSTSF (C) 9 STHFENSF M/P/N\A|N\S\T\S\F 100.0 9 6 3 1 0 b-ion bias 42.841274 1.422261 968.4115 968.414 -0.0032 -3.3 -0.8999999999999999 5.27 5790.8 nuORFdb_human ENST00000274030.10_1_4:120160492-120160642:+ ENST00000274030.10_1_4:120160492-120160642:+ USP53 5' uORF 5' uORF GN=USP53 5' uORF E_20221201_NO30_400nL_HLAc1_4e7_PDAC_Slot2-6_1_3524 Exploris_FAIMS VH20221207_MKP_FAIMS2022E_HLAc1_4e7_PDAC.32602.32602.2 2 1 1 11.12 5.51 6.7 5.62 80.6 7 0 0 2640000.0 Hydrogen 1 (-) 9 MPNANSTSF (C) 9 PGGQGDYAGF M P\N\A\N\S\T/S/F 77.8 7 5 2 0 0 b-ion bias 0.997 95.7 62.6 120.0 -70.0 484.7093 968.414 -0.003 -3.0 -0.3999999999999999 5.27 5790.8 nuORFdb_human ENST00000274030.10_1_4:120160492-120160642:+ ENST00000274030.10_1_4:120160492-120160642:+ USP53 5' uORF 5' uORF GN=USP53 5' uORF VH20221207_MKP_FAIMS2022E_HLAc1_4e7_PDAC SCP E_20221201_NO30_400nL_HLAc1_4e7_PDAC_Slot2-6_1_3524.28407.379.1 1 2 1 9.74 4.03 4.3 5.71 79.0 7 0 0 25600.0 Hydrogen 34 (L) 9 MPQPIPLSF (L) 9 VPCPLFLPS M/P Q|P\I|P-L\S\F 77.8 7 5 3 2 1 unbiased 98.140831 1.497639 1029.5426 1029.544 -0.0038 -3.7 -1.3000000000000003 5.27 8747.1 nuORFdb_human ENST00000481667.1_1_7:72954286-72958015:- ENST00000481667.1_1_7:72954286-72958015:-|ENST00000411832.5_1_7:72952271-72957931:- BCL7B 3' Overlap dORF 3' Overlap dORF GN=BCL7B 3' Overlap dORF E_20221201_NO30_400nL_HLAc1_4e7_PDAC_Slot2-6_1_3524 Eva-50mio-3fr AL20210827_EV_HLA_PDAC_PANFRO413_2D_50e6_W632_classI_fxn2.42913.43110.2 2 1 1 10.29 4.07 4.89 6.22 76.3 7 0 0 1290000.0 M34m Hydrogen 34 (L) 9 mPQPIPLSF (L) 9 IPGELWPSF m P\Q\P\I\P-L|S/F 77.8 7 5 2 1 1 b-ion bias m:Oxidized methionine 0.964 100.0 78.7 100.0 0.0 523.2737 1029.544 15.9964 1.5 1.2 5.27 8747.1 nuORFdb_human ENST00000481667.1_1_7:72954286-72958015:- ENST00000481667.1_1_7:72954286-72958015:-|ENST00000411832.5_1_7:72952271-72957931:- BCL7B 3' Overlap dORF 3' Overlap dORF GN=BCL7B 3' Overlap dORF AL20210827_EV_HLA_PDAC_PANFRO413_2D_50e6_W632_classI_fxn2 SCP E_20221201_NO30_400nL_HLAc1_4e7_PDAC_Slot2-6_1_3524.15717.1268.2 2 1 1 8.46 5.74 2.22 2.72 64.3 5 1 2 5940.0 Hydrogen 20 (I) 9 MPRKELELW (S) 9 LEMSPLGRIW M/P R K E\L\E|L\W 55.6 5 4 2 1 0 b-ion bias 54.298462 0.958499 601.3231 1201.64 -0.0011 -0.9 1.5 5.9 4671.4 nuORFdb_human ENST00000371368.5_1_1:54412201-54413639:+ ENST00000371368.5_1_1:54412201-54413639:+ LRRC42 5' uORF 5' uORF GN=LRRC42 5' uORF E_20221201_NO30_400nL_HLAc1_4e7_PDAC_Slot2-6_1_3524 SCP E_20221201_NO30_400nL_HLAc1_4e7_PDAC_Slot2-6_1_3524.12598.1352.2 2 2 1 16.96 9.12 7.84 7.84 95.8 7 1 2 93900.0 Hydrogen 4 (M) 9 MPSKKLILY (L) 9 MLLVLDLLY M/P/S/K/K L-I|L\Y 77.8 7 2 5 1 1 y-ion bias 43.521465 0.906655 546.828 1092.649 0.0002 0.1 2.5 9.7 1823.4 nuORFdb_human ENST00000316423.10_1_4:39512477-39515746:- ENST00000316423.10_1_4:39512477-39515746:- UGDH Out-of-Frame Out-of-Frame GN=UGDH Out-of-Frame E_20221201_NO30_400nL_HLAc1_4e7_PDAC_Slot2-6_1_3524 Exploris_FAIMS VH20221207_MKP_FAIMS2022E_HLAc1_4e7_PDAC.34384.34384.2 2 3 1 11.64 0.25 11.64 11.39 75.3 7 1 2 184000000.0 Hydrogen 4 (M) 9 MPSKKLILY (L) 9 YMPLKSKLL M/P/S K/K-L\I|L/Y 77.8 7 2 5 1 1 y-ion bias 0.997 96.9 65.2 12.54 -50.0 546.827 1092.649 -0.0019 -1.7 0.9000000000000001 9.7 1823.4 nuORFdb_human ENST00000316423.10_1_4:39512477-39515746:- ENST00000316423.10_1_4:39512477-39515746:- UGDH Out-of-Frame Out-of-Frame GN=UGDH Out-of-Frame VH20221207_MKP_FAIMS2022E_HLAc1_4e7_PDAC SCP E_20221201_NO30_400nL_HLAc1_4e7_PDAC_Slot2-6_1_3524.14970.1350.2 2 1 1 13.41 6.74 7.05 6.68 81.9 9 1 1 6500.0 Hydrogen 42 (K) 10 MPSSTGFRCF (-) 10 AFHCPKDSPM M/P/S|S/T|G/F/R/C\F 100.0 10 3 8 2 0 y-ion bias Carbamidomethylation C:Carbamidomethylation, 51.718174 0.907909 595.2587 1189.513 -0.0022 -1.8 0.5999999999999999 9.5 6006.8 nuORFdb_human ENST00000461469.5_1_7:156619430-156685195:- ENST00000461469.5_1_7:156619430-156685195:-|ENST00000415428.5_1_7:156619427-156685638:- LMBR1 3' Overlap dORF 3' Overlap dORF GN=LMBR1 3' Overlap dORF E_20221201_NO30_400nL_HLAc1_4e7_PDAC_Slot2-6_1_3524 Exploris_FAIMS VH20221207_MKP_FAIMS2022E_HLAc1_4e7_PDAC.25634.25634.2 2 1 1 9.28 4.03 5.55 5.25 63.2 7 0 0 1400000.0 M35m Hydrogen 35 (L) 9 mPTGVLYEH (-) 9 DTNRKYEH m/P/T/G V/L/Y/E/H 77.8 7 0 7 0 0 y-ion bias m:Oxidized methionine 0.932 93.2 52.15 120.0 -70.0 531.7483 1046.497 15.9918 -3.0 -0.3999999999999999 5.22 5396.4 nuORFdb_human ENST00000531120.5_1_11:32611159-32616479:+ ENST00000531120.5_1_11:32611159-32616479:+ EIF3M Out-of-Frame Out-of-Frame GN=EIF3M Out-of-Frame VH20221207_MKP_FAIMS2022E_HLAc1_4e7_PDAC SCP E_20221201_NO30_400nL_HLAc1_4e7_PDAC_Slot2-6_1_3524.28283.1426.2 2 2 1 19.38 8.65 10.18 10.73 94.6 8 1 1 165000.0 Hydrogen 1 (-) 9 MPTMRLLSF (V) 9 MRGWRRSF M/P/T/M/R\L|L|S\F 100.0 9 4 6 2 0 unbiased 97.712654 0.86017 548.2876 1095.569 -0.0002 -0.2 2.1999999999999997 9.5 17734.9 nuORFdb_human ENST00000472898.5_1_1:155981561-155990687:- ENST00000472898.5_1_1:155981561-155990687:-|ENST00000368311.5_1_1:155984733-155990735:-|ENST00000532074.5_1_1:155984857-155990687:-|ENST00000484320.6_1_1:155988041-155990687:- SSR2 3' Overlap dORF 3' Overlap dORF GN=SSR2 3' Overlap dORF E_20221201_NO30_400nL_HLAc1_4e7_PDAC_Slot2-6_1_3524 Eva-50mio-3fr AL20210827_EV_HLA_PDAC_PANFRO413_2D_50e6_W632_classI_fxn3.38765.38906.2 2 1 1 12.46 3.89 8.37 8.57 72.9 7 1 1 9470000.0 M1m Hydrogen 1 (-) 9 mPTMRLLSF (V) 9 mPTFSRILM m/P|T/M/R L\L|S/F 77.8 7 3 6 2 0 y-ion bias m:Oxidized methionine 0.992 79.9 70.55 100.0 0.0 556.2853 1095.569 15.9945 -0.4 -0.9 9.5 17734.9 nuORFdb_human ENST00000472898.5_1_1:155981561-155990687:- ENST00000472898.5_1_1:155981561-155990687:-|ENST00000368311.5_1_1:155984733-155990735:-|ENST00000532074.5_1_1:155984857-155990687:-|ENST00000484320.6_1_1:155988041-155990687:- SSR2 3' Overlap dORF 3' Overlap dORF GN=SSR2 3' Overlap dORF AL20210827_EV_HLA_PDAC_PANFRO413_2D_50e6_W632_classI_fxn3 Exploris_FAIMS VH20221207_MKP_FAIMS2022E_HLAc1_4e7_PDAC.61056.61204.2 2 1 1 13.25 4.65 8.96 8.6 76.1 7 1 1 38900000.0 M1m Hydrogen 1 (-) 9 mPTMRLLSF (V) 9 mPTFSRILM m/P|T/M/R L\L|S/F 77.8 7 3 6 2 0 y-ion bias m:Oxidized methionine 0.979 95.6 105.53 30.43 -70.0 556.2842 1095.569 15.9922 -2.5 0.10000000000000009 9.5 17734.9 nuORFdb_human ENST00000472898.5_1_1:155981561-155990687:- ENST00000472898.5_1_1:155981561-155990687:-|ENST00000368311.5_1_1:155984733-155990735:-|ENST00000532074.5_1_1:155984857-155990687:-|ENST00000484320.6_1_1:155988041-155990687:- SSR2 3' Overlap dORF 3' Overlap dORF GN=SSR2 3' Overlap dORF VH20221207_MKP_FAIMS2022E_HLAc1_4e7_PDAC Eva-50mio-3fr AL20210827_EV_HLA_PDAC_PANFRO413_2D_50e6_W632_classI_fxn3.30131.30131.2 2 1 1 9.41 2.74 9.41 6.67 72.8 7 1 1 87300000.0 M1m M4m Hydrogen 1 (-) 9 mPTmRLLSF (V) 9 mPTFSRILm m/P|T/m/R L\L/S/F 77.8 7 2 6 1 0 y-ion bias m:Oxidized methionine 0.992 97.8 55.45 15.44 0.0 564.2832 1095.569 31.9902 0.3 -0.2 9.5 17734.9 nuORFdb_human ENST00000472898.5_1_1:155981561-155990687:- ENST00000472898.5_1_1:155981561-155990687:-|ENST00000368311.5_1_1:155984733-155990735:-|ENST00000532074.5_1_1:155984857-155990687:-|ENST00000484320.6_1_1:155988041-155990687:- SSR2 3' Overlap dORF 3' Overlap dORF GN=SSR2 3' Overlap dORF AL20210827_EV_HLA_PDAC_PANFRO413_2D_50e6_W632_classI_fxn3 SCP E_20221201_NO30_400nL_HLAc1_4e7_PDAC_Slot2-6_1_3524.24416.163.1 1 1 1 9.8 3.5 7.56 6.29 81.1 6 0 0 4010.0 Hydrogen 29 (R) 11 MPTSSVVNMEF (-) 11 ELQLSMGnAMF M P T S S\V|V\N\M\E\F 54.5 6 6 1 1 0 b-ion bias 84.35038 1.624676 1241.5493 1241.554 -0.0041 -3.3 -0.8999999999999999 4.0 4518.1 nuORFdb_human ENST00000257575.8_1_12:117178177-117178294:+ ENST00000257575.8_1_12:117178177-117178294:+ RNFT2 5' uORF 5' uORF GN=RNFT2 5' uORF E_20221201_NO30_400nL_HLAc1_4e7_PDAC_Slot2-6_1_3524 Exploris_FAIMS VH20221207_MKP_FAIMS2022E_HLAc1_4e7_PDAC.57720.57720.2 2 1 1 9.76 4.13 8.76 5.63 77.1 6 0 0 24000000.0 Hydrogen 29 (R) 11 MPTSSVVNMEF (-) 11 EMNPLSAVSmF M P T-S S V-V\N\M\E/F 45.5 5 3 1 0 2 b-ion bias 0.994 78.2 100.65 37.76 -50.0 621.2794 1241.554 -0.0025 -2.0 0.6000000000000001 4.0 4518.1 nuORFdb_human ENST00000257575.8_1_12:117178177-117178294:+ ENST00000257575.8_1_12:117178177-117178294:+ RNFT2 5' uORF 5' uORF GN=RNFT2 5' uORF VH20221207_MKP_FAIMS2022E_HLAc1_4e7_PDAC Exploris_FAIMS VH20221207_MKP_FAIMS2022E_HLAc1_4e7_PDAC.46035.46182.2 2 1 1 8.96 8.96 8.96 68.1 6 0 0 4190000.0 M37m Hydrogen 29 (R) 11 MPTSSVVNmEF (-) 11 M P T\S S V\V\N\m-E/F 45.5 5 4 1 0 1 b-ion bias m:Oxidized methionine 0.962 100.0 82.7 115.76 -50.0 629.2769 1241.554 15.9924 -2.0 0.6000000000000001 4.0 4518.1 nuORFdb_human ENST00000257575.8_1_12:117178177-117178294:+ ENST00000257575.8_1_12:117178177-117178294:+ RNFT2 5' uORF 5' uORF GN=RNFT2 5' uORF VH20221207_MKP_FAIMS2022E_HLAc1_4e7_PDAC Eva-50mio-3fr AL20210827_EV_HLA_PDAC_PANFRO413_2D_50e6_W632_classI_fxn1.24984.25244.2 2 1 1 11.07 -1.03 11.07 12.1 77.3 8 0 0 21000000.0 M29m M37m Hydrogen 29 (R) 11 mPTSSVVNmEF (-) 11 mNPTnTVFGEF m P\T\S\S\V V\N\m\E/F 63.6 7 7 1 0 0 b-ion bias m:Oxidized methionine 0.961 100.0 46.03 100.0 0.0 637.2766 1241.554 31.9919 1.6 1.5 4.0 4518.1 nuORFdb_human ENST00000257575.8_1_12:117178177-117178294:+ ENST00000257575.8_1_12:117178177-117178294:+ RNFT2 5' uORF 5' uORF GN=RNFT2 5' uORF AL20210827_EV_HLA_PDAC_PANFRO413_2D_50e6_W632_classI_fxn1 Exploris_FAIMS VH20221207_MKP_FAIMS2022E_HLAc1_4e7_PDAC.26763.26763.2 2 1 1 9.82 6.26 6.0 3.56 85.6 6 1 1 2150000.0 Hydrogen 110 (K) 11 MPTTSTTSTRF (-) 11 MPTGQPSPSLSQ M/P|T|T/S/T T/S T R F 45.5 5 2 6 2 0 y-ion bias 0.968 100.0 53.83 120.0 -70.0 615.2936 1229.583 -0.0031 -2.6 0.0 9.5 13138.9 smORF_v4|nuORFdb_human LINC00938_12:46121131-46121493:- LINC00938_12:46121131-46121493:-|ENST00000609803.2_1_12:46121133-46121556:- LINC00938 lncRNA 12:46121131-46121493:- 12 RP11-352M15.2 lincRNA lncRNA GN=LINC00938 VH20221207_MKP_FAIMS2022E_HLAc1_4e7_PDAC SCP E_20221201_NO30_400nL_HLAc1_4e7_PDAC_Slot2-6_1_3524.19056.558.1 1 1 1 8.95 3.79 5.34 5.17 75.3 5 0 0 22200.0 Hydrogen 7 (S) 9 MPVGAWASP (-) 9 MVPGGDRSP M P V G\A|W\A\S\P 55.6 5 5 1 1 0 b-ion bias 65.831734 1.391411 915.437 915.439 -0.0023 -2.5 -0.10000000000000009 5.27 1543.9 nuORFdb_human ENST00000525250.5_1_11:821747-821795:+ ENST00000525250.5_1_11:821747-821795:+ PNPLA2 ncRNA Retained Intron lncRNA GN=PNPLA2 ncRNA Retained Intron E_20221201_NO30_400nL_HLAc1_4e7_PDAC_Slot2-6_1_3524 Exploris_FAIMS VH20221207_MKP_FAIMS2022E_HLAc1_4e7_PDAC.47354.47499.2 2 1 1 12.3 6.02 5.87 6.28 85.5 6 0 0 17400000.0 Hydrogen 7 (S) 9 MPVGAWASP (-) 9 MVPGGDRSP M P V\G\A-W\A|S/P 66.7 6 4 2 1 1 b-ion bias 0.995 91.0 84.77 23.42 -70.0 458.2222 915.439 -0.0021 -2.3 0.30000000000000027 5.27 1543.9 nuORFdb_human ENST00000525250.5_1_11:821747-821795:+ ENST00000525250.5_1_11:821747-821795:+ PNPLA2 ncRNA Retained Intron lncRNA GN=PNPLA2 ncRNA Retained Intron VH20221207_MKP_FAIMS2022E_HLAc1_4e7_PDAC SCP E_20221201_NO30_400nL_HLAc1_4e7_PDAC_Slot2-6_1_3524.29650.227.1 1 3 1 11.66 5.32 6.24 6.34 84.1 6 0 0 20300.0 Hydrogen 2 (M) 9 MQPSFWISF (-) 9 MQFSPWFSI M Q/P S|F\W\I\S\F 55.6 5 5 2 1 0 b-ion bias 102.439346 1.587431 1142.5267 1142.534 -0.0039 -3.4 -1.0 5.27 1273.5 nuORFdb_human ENST00000490738.5_1_1:6931821-6931851:+ ENST00000490738.5_1_1:6931821-6931851:+ CAMTA1 3' Overlap dORF 3' Overlap dORF GN=CAMTA1 3' Overlap dORF E_20221201_NO30_400nL_HLAc1_4e7_PDAC_Slot2-6_1_3524 40mio-rep1 E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep1_Slot1-04_1_3496.17156.1328.2 2 1 1 9.53 9.53 9.53 58.2 7 1 1 7070.0 C68c Hydrogen 65 (E) 9 MSDcVKLLY (S) 9 M/S/D|c/V|K L|L\Y 77.8 7 4 6 3 0 dual b/y bias c:Cysteinylation 59.261024 0.921539 595.7638 1128.543 61.9779 -3.9 -0.6999999999999997 5.59 13117.0 nuORFdb_human ENST00000453589.1_1_7:97599141-97601566:- ENST00000453589.1_1_7:97599141-97601566:-|ENST00000461592.1_1_7:5939975-5940047:+|ENST00000411858.1_1_7:6863774-6864307:- AC004967.7 Pseudogene Pseudogene GN=AC004967.7 Pseudogene E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep1_Slot1-04_1_3496 20mio-rep3 E_20221201_NO30_400nL_HLAc1_2e7_directIP_titration_rep4_Slot2-4_1_3519.16264.1280.2 2 2 1 9.43 5.18 9.43 54.7 7 1 1 5260.0 M28m Hydrogen 28 (W) 11 mSFTQKINSTW (F) 11 m S F|T|Q|K I\N|S/T\W 54.5 6 6 5 4 0 dual b/y bias m:Oxidized methionine 56.176437 0.951402 679.8199 1342.646 15.9867 -6.0 -0.20000000000000018 8.5 8763.2 nuORFdb_human ENST00000555408.5_1_12:56361645-56362592:+ ENST00000555408.5_1_12:56361645-56362592:+ CDK2 3' dORF 3' dORF GN=CDK2 3' dORF E_20221201_NO30_400nL_HLAc1_2e7_directIP_titration_rep4_Slot2-4_1_3519 40mio-rep4 E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep4_Slot2-5_1_3522.16268.1282.2 2 3 1 10.41 10.41 10.41 59.0 7 1 1 12800.0 M28m Hydrogen 28 (W) 11 mSFTQKINSTW (F) 11 m S/F|T|Q|K I\N|S T\W 45.5 5 6 5 4 0 dual b/y bias m:Oxidized methionine 56.181255 0.950257 679.8183 1342.646 15.9827 -9.0 -4.3 8.5 8763.2 nuORFdb_human ENST00000555408.5_1_12:56361645-56362592:+ ENST00000555408.5_1_12:56361645-56362592:+ CDK2 3' dORF 3' dORF GN=CDK2 3' dORF E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep4_Slot2-5_1_3522 40mio-rep1 E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep1_Slot1-04_1_3496.26288.442.1 1 3 1 12.93 6.18 7.91 6.75 82.8 5 0 0 16700.0 M11m Hydrogen 11 (G) 8 mSIAPAWW (-) 8 RSmAPNQAS m S I|A|P\A|W\W 62.5 5 5 3 3 0 dual b/y bias m:Oxidized methionine 90.815895 1.460207 977.4564 961.46 15.996 1.1 4.300000000000001 5.27 2130.5 nuORFdb_human ENST00000491410.1_1_1:52344287-52344344:- ENST00000491410.1_1_1:52344287-52344344:- NRDC ncRNA Processed Transcript lncRNA GN=NRDC ncRNA Processed Transcript E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep1_Slot1-04_1_3496 40mio-rep3 E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep3_Slot2-5_1_3521.6403.1341.2 2 1 1 10.67 5.89 6.46 4.78 64.6 6 0 0 1920.0 M32m M42m Hydrogen 32 (K) 11 mSLPTHATQTm (K) 11 mSLSSSKVmmT m S/L|P T H A/T|Q|T\m 45.5 5 4 5 3 0 unbiased m:Oxidized methionine 22.122351 0.91356 625.2786 1217.565 31.9854 -3.5 1.0999999999999996 6.49 7006.9 nuORFdb_human ENST00000423181.1_1_2:85836607-85838959:- ENST00000423181.1_1_2:85836607-85838959:- C2orf68 3' Overlap dORF 3' Overlap dORF GN=C2orf68 3' Overlap dORF E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep3_Slot2-5_1_3521 20mio-rep3 E_20221201_NO30_400nL_HLAc1_2e7_directIP_titration_rep4_Slot2-4_1_3519.23599.1390.2 2 1 1 9.6 9.6 9.6 63.4 6 1 1 5320.0 Hydrogen 1 (-) 9 MSLTTLIRY (C) 9 M/S/L|T|T/L I R-Y 66.7 6 2 5 2 1 y-ion bias 81.502922 0.882599 549.3016 1097.602 -0.0056 -5.1 0.7000000000000002 8.5 1370.7 nuORFdb_human ENST00000512276.5_1_3:141105742-141105778:+ ENST00000512276.5_1_3:141105742-141105778:+ ZBTB38 ncRNA Processed Transcript lncRNA GN=ZBTB38 ncRNA Processed Transcript E_20221201_NO30_400nL_HLAc1_2e7_directIP_titration_rep4_Slot2-4_1_3519 40mio-rep1 E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep1_Slot1-04_1_3496.23796.1366.2 2 2 1 15.76 3.78 11.29 11.98 87.5 8 1 1 69900.0 Hydrogen 1 (-) 9 MSLTTLIRY (C) 9 LHISIETVW M/S/L|T|T|L|I/R\Y 100.0 9 5 7 4 0 dual b/y bias 82.204811 0.89771 549.303 1097.602 -0.0036 -3.3 -0.09999999999999964 8.5 1370.7 nuORFdb_human ENST00000512276.5_1_3:141105742-141105778:+ ENST00000512276.5_1_3:141105742-141105778:+ ZBTB38 ncRNA Processed Transcript lncRNA GN=ZBTB38 ncRNA Processed Transcript E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep1_Slot1-04_1_3496 40mio-rep2 E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep2_Slot2-5_1_3520.23608.1394.2 2 2 1 12.65 1.57 11.05 11.08 71.9 8 1 1 26900.0 Hydrogen 1 (-) 9 MSLTTLIRY (C) 9 LHISIETVW M/S/L|T|T/L|I/R\Y 100.0 9 4 7 3 0 dual b/y bias 81.554153 0.88004 549.3021 1097.602 -0.0056 -5.1 -0.1999999999999993 8.5 1370.7 nuORFdb_human ENST00000512276.5_1_3:141105742-141105778:+ ENST00000512276.5_1_3:141105742-141105778:+ ZBTB38 ncRNA Processed Transcript lncRNA GN=ZBTB38 ncRNA Processed Transcript E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep2_Slot2-5_1_3520 40mio-rep3 E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep3_Slot2-5_1_3521.23641.1391.2 2 3 1 11.36 1.81 8.92 9.56 73.7 7 1 1 30200.0 Hydrogen 1 (-) 9 MSLTTLIRY (C) 9 SIMTYELKL M S/L|T|T/L/I/R-Y 77.8 7 2 6 2 1 y-ion bias 81.665817 0.881917 549.3015 1097.602 -0.0076 -6.9 -2.3000000000000007 8.5 1370.7 nuORFdb_human ENST00000512276.5_1_3:141105742-141105778:+ ENST00000512276.5_1_3:141105742-141105778:+ ZBTB38 ncRNA Processed Transcript lncRNA GN=ZBTB38 ncRNA Processed Transcript E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep3_Slot2-5_1_3521 40mio-rep4 E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep4_Slot2-5_1_3522.23641.1390.2 2 1 1 11.78 4.85 8.54 6.93 75.6 7 1 1 14200.0 Hydrogen 1 (-) 9 MSLTTLIRY (C) 9 MSLGGRHAGLV M/S/L|T|T/L/I|R Y 77.8 7 3 7 3 0 dual b/y bias 81.654778 0.882315 549.303 1097.602 -0.0036 -3.3 1.4000000000000004 8.5 1370.7 nuORFdb_human ENST00000512276.5_1_3:141105742-141105778:+ ENST00000512276.5_1_3:141105742-141105778:+ ZBTB38 ncRNA Processed Transcript lncRNA GN=ZBTB38 ncRNA Processed Transcript E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep4_Slot2-5_1_3522 5mio-rep1 E_20221201_NO30_400nL_HLAc1_5e6_directIP_titration_rep1_Slot1-37_1_3502.23750.1395.2 2 1 1 7.36 7.36 7.36 60.7 7 1 1 1920.0 Hydrogen 1 (-) 9 MSLTTLIRY (C) 9 M/S/L|T/T/L|I R\Y 77.8 7 3 6 2 0 y-ion bias 82.030113 0.879186 549.3028 1097.602 -0.0036 -3.3 3.7 8.5 1370.7 nuORFdb_human ENST00000512276.5_1_3:141105742-141105778:+ ENST00000512276.5_1_3:141105742-141105778:+ ZBTB38 ncRNA Processed Transcript lncRNA GN=ZBTB38 ncRNA Processed Transcript E_20221201_NO30_400nL_HLAc1_5e6_directIP_titration_rep1_Slot1-37_1_3502 10mio-rep1 E_20221201_NO30_400nL_HLAc1_1e7_directIP_titration_rep1_Slot2-3_1_3512.19932.1399.2 2 2 1 13.49 5.2 13.49 8.29 74.8 8 1 1 7630.0 M1m Hydrogen 1 (-) 9 mSLTTLIRY (C) 9 FAETTLYRI m/S/L/T|T/L/I/R\Y 100.0 9 2 7 1 0 y-ion bias m:Oxidized methionine 68.852592 0.877134 557.2969 1097.602 15.9844 -9.5 -1.7999999999999998 8.5 1370.7 nuORFdb_human ENST00000512276.5_1_3:141105742-141105778:+ ENST00000512276.5_1_3:141105742-141105778:+ ZBTB38 ncRNA Processed Transcript lncRNA GN=ZBTB38 ncRNA Processed Transcript E_20221201_NO30_400nL_HLAc1_1e7_directIP_titration_rep1_Slot2-3_1_3512 10mio-rep2 E_20221201_NO30_400nL_HLAc1_1e7_directIP_titration_rep2_Slot2-3_1_3513.19873.1396.2 2 3 1 15.26 6.47 9.83 8.78 81.6 6 1 1 13500.0 M1m Hydrogen 1 (-) 9 mSLTTLIRY (C) 9 FAETTLYRI m S|L|T|T/L|I|R Y 55.6 5 5 6 5 0 dual b/y bias m:Oxidized methionine 68.644005 0.878763 557.298 1097.602 15.9864 -7.7 -1.0 8.5 1370.7 nuORFdb_human ENST00000512276.5_1_3:141105742-141105778:+ ENST00000512276.5_1_3:141105742-141105778:+ ZBTB38 ncRNA Processed Transcript lncRNA GN=ZBTB38 ncRNA Processed Transcript E_20221201_NO30_400nL_HLAc1_1e7_directIP_titration_rep2_Slot2-3_1_3513 10mio-rep3 E_20221201_NO30_400nL_HLAc1_1e7_directIP_titration_rep3_Slot2-3_1_3514.19823.1414.2 2 3 1 16.19 6.3 10.18 9.89 82.7 8 1 1 17500.0 M1m Hydrogen 1 (-) 9 mSLTTLIRY (C) 9 mSLGGRHAGLV m/S|L|T/T/L|I|R\Y 100.0 9 5 7 4 0 dual b/y bias m:Oxidized methionine 68.475502 0.867874 557.2992 1097.602 15.9884 -5.9 0.39999999999999947 8.5 1370.7 nuORFdb_human ENST00000512276.5_1_3:141105742-141105778:+ ENST00000512276.5_1_3:141105742-141105778:+ ZBTB38 ncRNA Processed Transcript lncRNA GN=ZBTB38 ncRNA Processed Transcript E_20221201_NO30_400nL_HLAc1_1e7_directIP_titration_rep3_Slot2-3_1_3514 20mio-rep1 E_20221201_NO30_400nL_HLAc1_2e7_directIP_titration_rep1_Slot2-4_1_3515.19858.1396.2 2 2 1 16.77 7.08 9.7 9.7 89.4 8 1 1 14800.0 M1m Hydrogen 1 (-) 9 mSLTTLIRY (C) 9 mSLSLISLSF m/S|L|T|T/L|I|R\Y 100.0 9 6 7 5 0 dual b/y bias m:Oxidized methionine 68.578346 0.87908 557.2984 1097.602 15.9864 -7.7 -1.5 8.5 1370.7 nuORFdb_human ENST00000512276.5_1_3:141105742-141105778:+ ENST00000512276.5_1_3:141105742-141105778:+ ZBTB38 ncRNA Processed Transcript lncRNA GN=ZBTB38 ncRNA Processed Transcript E_20221201_NO30_400nL_HLAc1_2e7_directIP_titration_rep1_Slot2-4_1_3515 20mio-rep2 E_20221201_NO30_400nL_HLAc1_2e7_directIP_titration_rep3_Slot2-4_1_3518.19902.1394.2 2 3 1 13.37 5.26 8.6 8.11 83.6 8 1 1 22900.0 M1m Hydrogen 1 (-) 9 mSLTTLIRY (C) 9 FAETTLYRI m/S/L|T|T/L\I/R\Y 100.0 9 4 6 2 0 unbiased m:Oxidized methionine 68.746353 0.880402 557.2998 1097.602 15.9904 -4.1 2.2 8.5 1370.7 nuORFdb_human ENST00000512276.5_1_3:141105742-141105778:+ ENST00000512276.5_1_3:141105742-141105778:+ ZBTB38 ncRNA Processed Transcript lncRNA GN=ZBTB38 ncRNA Processed Transcript E_20221201_NO30_400nL_HLAc1_2e7_directIP_titration_rep3_Slot2-4_1_3518 20mio-rep3 E_20221201_NO30_400nL_HLAc1_2e7_directIP_titration_rep4_Slot2-4_1_3519.19924.1395.2 2 3 1 16.31 5.98 11.5 10.33 85.6 8 1 1 23000.0 M1m Hydrogen 1 (-) 9 mSLTTLIRY (C) 9 mLSQKLKSY m/S|L|T/T/L/I|R\Y 100.0 9 4 7 3 0 dual b/y bias m:Oxidized methionine 68.813179 0.87974 557.2995 1097.602 15.9904 -4.1 1.7000000000000002 8.5 1370.7 nuORFdb_human ENST00000512276.5_1_3:141105742-141105778:+ ENST00000512276.5_1_3:141105742-141105778:+ ZBTB38 ncRNA Processed Transcript lncRNA GN=ZBTB38 ncRNA Processed Transcript E_20221201_NO30_400nL_HLAc1_2e7_directIP_titration_rep4_Slot2-4_1_3519 40mio-rep1 E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep1_Slot1-04_1_3496.20176.1360.2 2 2 1 17.85 7.27 12.06 10.58 88.1 7 1 1 57200.0 M1m Hydrogen 1 (-) 9 mSLTTLIRY (C) 9 FAETTLYRI m/S|L|T|T/L|I|R Y 77.8 7 5 7 5 0 dual b/y bias m:Oxidized methionine 69.698296 0.90112 557.3009 1097.602 15.9924 -2.3 0.9000000000000004 8.5 1370.7 nuORFdb_human ENST00000512276.5_1_3:141105742-141105778:+ ENST00000512276.5_1_3:141105742-141105778:+ ZBTB38 ncRNA Processed Transcript lncRNA GN=ZBTB38 ncRNA Processed Transcript E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep1_Slot1-04_1_3496 40mio-rep2 E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep2_Slot2-5_1_3520.19910.1374.2 2 3 1 18.68 7.01 11.43 11.66 93.3 8 1 1 70700.0 M1m Hydrogen 1 (-) 9 mSLTTLIRY (C) 9 mSLGGRHAGLV m/S|L|T|T/L|I|R\Y 100.0 9 6 7 5 0 dual b/y bias m:Oxidized methionine 68.779449 0.892768 557.2993 1097.602 15.9884 -5.9 -1.0 8.5 1370.7 nuORFdb_human ENST00000512276.5_1_3:141105742-141105778:+ ENST00000512276.5_1_3:141105742-141105778:+ ZBTB38 ncRNA Processed Transcript lncRNA GN=ZBTB38 ncRNA Processed Transcript E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep2_Slot2-5_1_3520 40mio-rep3 E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep3_Slot2-5_1_3521.19946.1374.2 2 3 1 18.25 6.86 11.39 11.39 89.6 8 1 1 61600.0 M1m Hydrogen 1 (-) 9 mSLTTLIRY (C) 9 mSLSLISLSF m/S|L|T|T/L|I|R\Y 100.0 9 6 7 5 0 dual b/y bias m:Oxidized methionine 68.903671 0.89241 557.3009 1097.602 15.9924 -2.3 2.3 8.5 1370.7 nuORFdb_human ENST00000512276.5_1_3:141105742-141105778:+ ENST00000512276.5_1_3:141105742-141105778:+ ZBTB38 ncRNA Processed Transcript lncRNA GN=ZBTB38 ncRNA Processed Transcript E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep3_Slot2-5_1_3521 40mio-rep4 E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep4_Slot2-5_1_3522.19967.1375.2 2 3 1 18.08 6.91 11.51 11.18 91.9 8 1 1 55300.0 M1m Hydrogen 1 (-) 9 mSLTTLIRY (C) 9 FAETTLYRI m/S|L|T|T/L|I|R\Y 100.0 9 6 7 5 0 dual b/y bias m:Oxidized methionine 68.959053 0.892008 557.3003 1097.602 15.9904 -4.1 0.6000000000000005 8.5 1370.7 nuORFdb_human ENST00000512276.5_1_3:141105742-141105778:+ ENST00000512276.5_1_3:141105742-141105778:+ ZBTB38 ncRNA Processed Transcript lncRNA GN=ZBTB38 ncRNA Processed Transcript E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep4_Slot2-5_1_3522 5mio-rep1 E_20221201_NO30_400nL_HLAc1_5e6_directIP_titration_rep1_Slot1-37_1_3502.20136.1396.2 2 2 1 12.11 6.15 5.67 5.97 72.8 7 1 1 6540.0 M1m Hydrogen 1 (-) 9 mSLTTLIRY (C) 9 mSLSLISLSF m S|L|T/T/L/I/R\Y 77.8 7 3 6 2 0 y-ion bias m:Oxidized methionine 69.548096 0.878944 557.2978 1097.602 15.9864 -7.7 -0.7000000000000002 8.5 1370.7 nuORFdb_human ENST00000512276.5_1_3:141105742-141105778:+ ENST00000512276.5_1_3:141105742-141105778:+ ZBTB38 ncRNA Processed Transcript lncRNA GN=ZBTB38 ncRNA Processed Transcript E_20221201_NO30_400nL_HLAc1_5e6_directIP_titration_rep1_Slot1-37_1_3502 40mio-rep2 E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep2_Slot2-5_1_3520.7954.1475.2 2 1 1 8.87 8.87 8.87 64.2 6 0 1 6410.0 M18m Hydrogen 18 (K) 9 mSNGPQRPW (-) 9 m/S/N/G|P Q/R|P W 55.6 5 2 6 2 0 y-ion bias m:Oxidized methionine 27.481564 0.829456 544.7475 1072.499 15.9894 -5.0 -0.09999999999999964 9.5 3047.5 nuORFdb_human ENST00000295802.8_1_2:85581494-85581575:- ENST00000295802.8_1_2:85581494-85581575:- RETSAT Out-of-Frame Out-of-Frame GN=RETSAT Out-of-Frame E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep2_Slot2-5_1_3520 40mio-rep1 E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep1_Slot1-04_1_3496.14853.1507.2 2 2 1 12.42 6.86 3.19 5.56 83.2 7 1 1 15000.0 Hydrogen 5 (P) 8 MSTLPSKW (K) 8 mATLnNIR M/S/T|L|P/S/K\W 100.0 8 3 6 2 0 y-ion bias 51.3069 0.809206 475.2421 949.481 -0.0044 -4.7 -1.5 8.5 43858.6 nuORFdb_human ENST00000544902.5_1_13:114277566-114294579:+ ENST00000544902.5_1_13:114277566-114294579:+ TFDP1 3' Overlap dORF 3' Overlap dORF GN=TFDP1 3' Overlap dORF E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep1_Slot1-04_1_3496 40mio-rep2 E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep2_Slot2-5_1_3520.11936.1507.2 2 1 1 8.97 6.31 2.05 2.65 59.4 6 1 1 6480.0 M5m Hydrogen 5 (P) 8 mSTLPSKW (K) 8 mQKISTQN m/S/T|L/P/S K\W 75.0 6 2 5 1 0 y-ion bias m:Oxidized methionine 41.232018 0.808984 483.241 949.481 15.9936 -1.4 3.5000000000000004 8.5 43858.6 nuORFdb_human ENST00000544902.5_1_13:114277566-114294579:+ ENST00000544902.5_1_13:114277566-114294579:+ TFDP1 3' Overlap dORF 3' Overlap dORF GN=TFDP1 3' Overlap dORF E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep2_Slot2-5_1_3520 40mio-rep4 E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep4_Slot2-5_1_3522.11973.1510.2 2 1 1 8.7 2.35 8.7 53.6 6 1 1 8950.0 M5m Hydrogen 5 (P) 8 mSTLPSKW (K) 8 m S|T/L|P/S\K\W 75.0 6 4 4 2 0 unbiased m:Oxidized methionine 41.34766 0.807394 483.2411 949.481 15.9936 -1.4 3.3000000000000003 8.5 43858.6 nuORFdb_human ENST00000544902.5_1_13:114277566-114294579:+ ENST00000544902.5_1_13:114277566-114294579:+ TFDP1 3' Overlap dORF 3' Overlap dORF GN=TFDP1 3' Overlap dORF E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep4_Slot2-5_1_3522 20mio-rep1 E_20221201_NO30_400nL_HLAc1_2e7_directIP_titration_rep1_Slot2-4_1_3515.7626.1557.2 2 1 1 8.84 4.95 2.68 3.89 81.2 6 1 1 9870.0 Hydrogen 33 (L) 8 MTAEPRSL (S) 8 MTANQVQI M/T|A|E|P R-S-L 75.0 6 3 4 3 2 dual b/y bias 26.345499 0.777368 452.7281 904.456 -0.007 -7.7 -1.5 5.75 9046.3 nuORFdb_human ENST00000451648.1_1_20:61294700-61297915:- ENST00000451648.1_1_20:61294700-61297915:-|ENST00000411824.1_1_20:61294700-61297915:-|ENST00000433126.1_1_20:61297286-61297915:- SLCO4A1-AS1 Antisense lncRNA GN=SLCO4A1-AS1 Antisense E_20221201_NO30_400nL_HLAc1_2e7_directIP_titration_rep1_Slot2-4_1_3515 20mio-rep2 E_20221201_NO30_400nL_HLAc1_2e7_directIP_titration_rep3_Slot2-4_1_3518.7773.1550.2 2 1 1 10.72 6.28 3.55 4.44 83.2 6 1 1 7280.0 Hydrogen 33 (L) 8 MTAEPRSL (S) 8 MTANQVQI M/T|A|E|P R\S\L 75.0 6 5 4 3 0 dual b/y bias 26.853682 0.782135 452.727 904.456 -0.009 -9.9 -3.6000000000000005 5.75 9046.3 nuORFdb_human ENST00000451648.1_1_20:61294700-61297915:- ENST00000451648.1_1_20:61294700-61297915:-|ENST00000411824.1_1_20:61294700-61297915:-|ENST00000433126.1_1_20:61297286-61297915:- SLCO4A1-AS1 Antisense lncRNA GN=SLCO4A1-AS1 Antisense E_20221201_NO30_400nL_HLAc1_2e7_directIP_titration_rep3_Slot2-4_1_3518 40mio-rep1 E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep1_Slot1-04_1_3496.7933.1505.2 2 3 1 14.06 8.51 4.64 5.55 93.8 5 1 1 93600.0 Hydrogen 33 (L) 8 MTAEPRSL (S) 8 MTANQVQI M/T|A|E|P/R S L 62.5 5 3 5 3 0 dual b/y bias 27.409548 0.810356 452.7291 904.456 -0.005 -5.5 -2.3 5.75 9046.3 nuORFdb_human ENST00000451648.1_1_20:61294700-61297915:- ENST00000451648.1_1_20:61294700-61297915:-|ENST00000411824.1_1_20:61294700-61297915:-|ENST00000433126.1_1_20:61297286-61297915:- SLCO4A1-AS1 Antisense lncRNA GN=SLCO4A1-AS1 Antisense E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep1_Slot1-04_1_3496 40mio-rep2 E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep2_Slot2-5_1_3520.7627.1540.2 2 2 1 10.37 6.16 3.18 4.21 81.1 5 1 1 39700.0 Hydrogen 33 (L) 8 MTAEPRSL (S) 8 MTANQVQI M/T|A|E|P R\S L 50.0 4 4 4 3 0 dual b/y bias 26.351963 0.788326 452.7293 904.456 -0.005 -5.5 -0.5999999999999996 5.75 9046.3 nuORFdb_human ENST00000451648.1_1_20:61294700-61297915:- ENST00000451648.1_1_20:61294700-61297915:-|ENST00000411824.1_1_20:61294700-61297915:-|ENST00000433126.1_1_20:61297286-61297915:- SLCO4A1-AS1 Antisense lncRNA GN=SLCO4A1-AS1 Antisense E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep2_Slot2-5_1_3520 40mio-rep4 E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep4_Slot2-5_1_3522.7659.1517.2 2 1 1 10.91 6.64 4.14 4.26 89.4 5 1 1 30400.0 Hydrogen 33 (L) 8 MTAEPRSL (S) 8 METAPRVT M/T|A/E|P/R S L 62.5 5 2 5 2 0 y-ion bias 26.455563 0.803018 452.7288 904.456 -0.005 -5.5 -0.7999999999999998 5.75 9046.3 nuORFdb_human ENST00000451648.1_1_20:61294700-61297915:- ENST00000451648.1_1_20:61294700-61297915:-|ENST00000411824.1_1_20:61294700-61297915:-|ENST00000433126.1_1_20:61297286-61297915:- SLCO4A1-AS1 Antisense lncRNA GN=SLCO4A1-AS1 Antisense E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep4_Slot2-5_1_3522 20mio-rep1 E_20221201_NO30_400nL_HLAc1_2e7_directIP_titration_rep1_Slot2-4_1_3515.5177.1557.2 2 1 1 11.66 7.39 3.37 4.28 79.7 5 1 1 7330.0 M33m Hydrogen 33 (L) 8 mTAEPRSL (S) 8 mTANQVQI m/T|A|E|P R S\L 62.5 5 4 4 3 0 dual b/y bias m:Oxidized methionine 17.890961 0.777828 460.7261 904.456 15.989 -6.4 -0.20000000000000018 5.75 9046.3 nuORFdb_human ENST00000451648.1_1_20:61294700-61297915:- ENST00000451648.1_1_20:61294700-61297915:-|ENST00000411824.1_1_20:61294700-61297915:-|ENST00000433126.1_1_20:61297286-61297915:- SLCO4A1-AS1 Antisense lncRNA GN=SLCO4A1-AS1 Antisense E_20221201_NO30_400nL_HLAc1_2e7_directIP_titration_rep1_Slot2-4_1_3515 20mio-rep3 E_20221201_NO30_400nL_HLAc1_2e7_directIP_titration_rep4_Slot2-4_1_3519.5286.1548.2 2 3 1 11.56 7.67 2.51 3.89 78.8 5 1 1 11400.0 M33m Hydrogen 33 (L) 8 mTAEPRSL (S) 8 mTANQVQI m/T|A|E|P R S\L 62.5 5 4 4 3 0 dual b/y bias m:Oxidized methionine 18.266592 0.783286 460.7268 904.456 15.991 -4.2 1.5999999999999996 5.75 9046.3 nuORFdb_human ENST00000451648.1_1_20:61294700-61297915:- ENST00000451648.1_1_20:61294700-61297915:-|ENST00000411824.1_1_20:61294700-61297915:-|ENST00000433126.1_1_20:61297286-61297915:- SLCO4A1-AS1 Antisense lncRNA GN=SLCO4A1-AS1 Antisense E_20221201_NO30_400nL_HLAc1_2e7_directIP_titration_rep4_Slot2-4_1_3519 40mio-rep1 E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep1_Slot1-04_1_3496.5518.1535.2 2 1 1 11.15 6.73 2.73 4.42 78.7 5 1 1 7880.0 M33m Hydrogen 33 (L) 8 mTAEPRSL (S) 8 mTANQVQI m/T|A/E|P R S\L 62.5 5 3 4 2 0 unbiased m:Oxidized methionine 19.071625 0.791454 460.7274 904.456 15.991 -4.2 -1.0 5.75 9046.3 nuORFdb_human ENST00000451648.1_1_20:61294700-61297915:- ENST00000451648.1_1_20:61294700-61297915:-|ENST00000411824.1_1_20:61294700-61297915:-|ENST00000433126.1_1_20:61297286-61297915:- SLCO4A1-AS1 Antisense lncRNA GN=SLCO4A1-AS1 Antisense E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep1_Slot1-04_1_3496 40mio-rep2 E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep2_Slot2-5_1_3520.5260.1544.2 2 1 1 7.24 2.71 3.99 4.53 68.8 6 1 1 15400.0 M33m Hydrogen 33 (L) 8 mTAEPRSL (S) 8 mTANQVQI m/T/A/E|P/R S\L 75.0 6 2 5 1 0 y-ion bias m:Oxidized methionine 18.177679 0.785561 460.7255 904.456 15.989 -6.4 -1.5 5.75 9046.3 nuORFdb_human ENST00000451648.1_1_20:61294700-61297915:- ENST00000451648.1_1_20:61294700-61297915:-|ENST00000411824.1_1_20:61294700-61297915:-|ENST00000433126.1_1_20:61297286-61297915:- SLCO4A1-AS1 Antisense lncRNA GN=SLCO4A1-AS1 Antisense E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep2_Slot2-5_1_3520 40mio-rep3 E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep3_Slot2-5_1_3521.5210.1548.2 2 3 1 11.67 6.77 2.67 4.9 80.7 6 1 1 22800.0 M33m Hydrogen 33 (L) 8 mTAEPRSL (S) 8 mTANQVQI m/T|A|E|P R\S\L 75.0 6 5 4 3 0 dual b/y bias m:Oxidized methionine 18.002953 0.783422 460.7265 904.456 15.989 -6.4 -1.8000000000000007 5.75 9046.3 nuORFdb_human ENST00000451648.1_1_20:61294700-61297915:- ENST00000451648.1_1_20:61294700-61297915:-|ENST00000411824.1_1_20:61294700-61297915:-|ENST00000433126.1_1_20:61297286-61297915:- SLCO4A1-AS1 Antisense lncRNA GN=SLCO4A1-AS1 Antisense E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep3_Slot2-5_1_3521 20mio-rep2 E_20221201_NO30_400nL_HLAc1_2e7_directIP_titration_rep3_Slot2-4_1_3518.19128.1405.2 2 1 1 13.44 5.41 7.51 8.02 78.8 6 1 1 5900.0 M33m Hydrogen 33 (K) 9 mTKTILYGF (-) 9 WRTFIYGF m T/K T\I|L|Y\G\F 55.6 5 5 3 2 0 unbiased m:Oxidized methionine 66.074364 0.873341 545.2841 1073.57 15.9907 -3.8 2.5 8.34 5043.1 nuORFdb_human ENST00000568717.1_1_16:74334023-74334994:+ ENST00000568717.1_1_16:74334023-74334994:+ PSMD7 3' dORF 3' dORF GN=PSMD7 3' dORF E_20221201_NO30_400nL_HLAc1_2e7_directIP_titration_rep3_Slot2-4_1_3518 40mio-rep3 E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep3_Slot2-5_1_3521.19020.1376.2 2 1 1 13.9 6.59 6.72 7.31 74.8 5 1 1 6300.0 M33m Hydrogen 33 (K) 9 mTKTILYGF (-) 9 WRTFIYGF m T K/T I|L|Y|G\F 44.4 4 4 4 3 0 dual b/y bias m:Oxidized methionine 65.702888 0.891294 545.2829 1073.57 15.9887 -5.7 -1.1000000000000005 8.34 5043.1 nuORFdb_human ENST00000568717.1_1_16:74334023-74334994:+ ENST00000568717.1_1_16:74334023-74334994:+ PSMD7 3' dORF 3' dORF GN=PSMD7 3' dORF E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep3_Slot2-5_1_3521 40mio-rep4 E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep4_Slot2-5_1_3522.15065.1499.2 2 1 1 10.19 6.43 4.13 3.76 71.0 6 1 1 7210.0 Hydrogen 1 (-) 9 MTLAALRDA (E) 9 MTLADGRVV M/T|L|A/A L R\D\A 66.7 6 4 4 2 0 unbiased 52.027916 0.814368 481.2566 961.514 -0.0068 -7.1 -2.3999999999999995 5.59 42760.0 nuORFdb_human ENST00000299353.6_1_10:104622682-104660454:+ ENST00000299353.6_1_10:104622682-104660454:+ BORCS7-ASMT 3' Overlap dORF 3' Overlap dORF GN=BORCS7-ASMT 3' Overlap dORF E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep4_Slot2-5_1_3522 20mio-rep1 E_20221201_NO30_400nL_HLAc1_2e7_directIP_titration_rep1_Slot2-4_1_3515.10646.1509.2 2 2 1 8.82 5.77 3.81 3.05 56.9 6 1 1 11200.0 M1m Hydrogen 1 (-) 9 mTLAALRDA (E) 9 TmRVLPSSA m T/L|A/A\L R\D\A 55.6 5 4 3 1 0 unbiased m:Oxidized methionine 36.771503 0.807626 489.2559 961.514 15.9912 -3.8 2.4000000000000004 5.59 42760.0 nuORFdb_human ENST00000299353.6_1_10:104622682-104660454:+ ENST00000299353.6_1_10:104622682-104660454:+ BORCS7-ASMT 3' Overlap dORF 3' Overlap dORF GN=BORCS7-ASMT 3' Overlap dORF E_20221201_NO30_400nL_HLAc1_2e7_directIP_titration_rep1_Slot2-4_1_3515 40mio-rep1 E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep1_Slot1-04_1_3496.28033.1230.2 2 1 1 7.5 7.5 7.5 57.8 9 0 0 5590.0 Hydrogen 8 (R) 11 MTLSMSELHLW (A) 11 M/T/L|S|M/S E/L/H-L\W 81.8 9 3 7 2 1 y-ion bias 96.846436 0.982267 674.3243 1347.644 -0.0028 -2.1 1.1 5.22 6120.8 nuORFdb_human ENST00000500337.6_1_5:43454122-43476351:- ENST00000500337.6_1_5:43454122-43476351:- TMEM267 5' uORF 5' uORF GN=TMEM267 5' uORF E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep1_Slot1-04_1_3496 SCP E_20221201_NO30_400nL_HLAc1_4e7_PDAC_Slot2-6_1_3524.10173.1395.2 2 1 1 9.69 6.68 5.78 3.01 70.0 5 0 0 7650.0 M8m Hydrogen 8 (W) 11 mTTATPGAHPF (P) 11 FDSATVCSLF m T T|A|T|P|G A H P\F 36.4 4 5 4 4 0 dual b/y bias m:Oxidized methionine 35.14587 0.879428 573.7632 1130.53 15.9888 -5.3 -2.9 6.49 8240.3 nuORFdb_human ENST00000368921.4_1_1:151031777-151031999:+ ENST00000368921.4_1_1:151031777-151031999:+ MLLT11 5' uORF 5' uORF GN=MLLT11 5' uORF E_20221201_NO30_400nL_HLAc1_4e7_PDAC_Slot2-6_1_3524 40mio-rep1 E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep1_Slot1-04_1_3496.28959.1261.2 2 1 1 13.18 2.42 8.76 10.76 77.6 9 1 1 3480.0 Hydrogen 1 (-) 11 MTTDVLSLLRL (L) 11 CKQMLRSILL M/T/T/D|V|L|S/L|L R\L 81.8 9 5 8 4 0 dual b/y bias 100.044174 0.963046 631.3607 1261.718 -0.0037 -2.9 0.30000000000000027 5.59 1660.0 nuORFdb_human ENST00000295955.13_3_4:39460055-39460550:- ENST00000295955.13_3_4:39460055-39460550:- RPL9 5' Overlap uORF 5' Overlap uORF GN=RPL9 5' Overlap uORF E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep1_Slot1-04_1_3496 40mio-rep1 E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep1_Slot1-04_1_3496.28551.1265.2 2 2 1 13.31 0.89 9.51 12.42 67.1 9 1 1 7930.0 M1m Hydrogen 1 (-) 11 mTTDVLSLLRL (L) 11 MMAMVILVNKK m T|T|D|V|L|S|L/L/R\L 81.8 9 7 8 6 0 dual b/y bias m:Oxidized methionine 98.635468 0.9606 639.3572 1261.718 15.9883 -5.2 -2.0 5.59 1660.0 nuORFdb_human ENST00000295955.13_3_4:39460055-39460550:- ENST00000295955.13_3_4:39460055-39460550:- RPL9 5' Overlap uORF 5' Overlap uORF GN=RPL9 5' Overlap uORF E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep1_Slot1-04_1_3496 1mio-rep1 E_20221201_NO30_400nL_HLAc1_1e6_directIP_titration_rep1_Slot1-06_1_3498.22826.472.1 1 2 1 8.02 4.55 2.56 3.47 68.6 6 0 0 3410.0 M1m Hydrogen 1 (-) 9 mVLGADAVW (L) 9 GLDmVGLAAD m V L\G\A\D\A\V\W 66.7 6 6 0 0 0 b-ion bias m:Oxidized methionine 78.828659 1.442723 977.4697 961.481 15.9888 -6.2 -1.2999999999999998 3.8 6507.4 nuORFdb_human ENST00000452197.5_1_1:173834379-173836179:- ENST00000452197.5_1_1:173834379-173836179:-|ENST00000432536.5_2_1:173835321-173836200:-|ENST00000451607.5_2_1:173835321-173836299:-|ENST00000430245.5_1_1:173835321-173837929:-|ENST00000458220.1_1_1:173835633-173836179:-|ENST00000431268.5_1_1:173835892-173836179:- GAS5 lincRNA lncRNA GN=GAS5 lincRNA E_20221201_NO30_400nL_HLAc1_1e6_directIP_titration_rep1_Slot1-06_1_3498 10mio-rep2 E_20221201_NO30_400nL_HLAc1_1e7_directIP_titration_rep2_Slot2-3_1_3513.22522.485.1 1 5 1 15.61 9.0 5.77 6.61 89.6 7 0 0 21700.0 M1m Hydrogen 1 (-) 9 mVLGADAVW (L) 9 mVAAEVGVW m V|L|G\A\D\A\V\W 77.8 7 7 2 2 0 b-ion bias m:Oxidized methionine 77.791718 1.434918 977.4721 961.481 15.9908 -4.2 2.5 3.8 6507.4 nuORFdb_human ENST00000452197.5_1_1:173834379-173836179:- ENST00000452197.5_1_1:173834379-173836179:-|ENST00000432536.5_2_1:173835321-173836200:-|ENST00000451607.5_2_1:173835321-173836299:-|ENST00000430245.5_1_1:173835321-173837929:-|ENST00000458220.1_1_1:173835633-173836179:-|ENST00000431268.5_1_1:173835892-173836179:- GAS5 lincRNA lncRNA GN=GAS5 lincRNA E_20221201_NO30_400nL_HLAc1_1e7_directIP_titration_rep2_Slot2-3_1_3513 10mio-rep3 E_20221201_NO30_400nL_HLAc1_1e7_directIP_titration_rep3_Slot2-3_1_3514.22479.485.1 1 2 1 14.3 8.33 4.88 5.97 86.0 7 0 0 15500.0 M1m Hydrogen 1 (-) 9 mVLGADAVW (L) 9 LNAEVmVW m V/L|G\A\D\A\V\W 77.8 7 6 2 1 0 b-ion bias m:Oxidized methionine 77.648407 1.434921 977.4715 961.481 15.9908 -4.2 2.0999999999999996 3.8 6507.4 nuORFdb_human ENST00000452197.5_1_1:173834379-173836179:- ENST00000452197.5_1_1:173834379-173836179:-|ENST00000432536.5_2_1:173835321-173836200:-|ENST00000451607.5_2_1:173835321-173836299:-|ENST00000430245.5_1_1:173835321-173837929:-|ENST00000458220.1_1_1:173835633-173836179:-|ENST00000431268.5_1_1:173835892-173836179:- GAS5 lincRNA lncRNA GN=GAS5 lincRNA E_20221201_NO30_400nL_HLAc1_1e7_directIP_titration_rep3_Slot2-3_1_3514 20mio-rep1 E_20221201_NO30_400nL_HLAc1_2e7_directIP_titration_rep1_Slot2-4_1_3515.22477.485.1 1 6 1 14.92 8.24 5.79 6.67 87.1 7 0 0 52000.0 M1m Hydrogen 1 (-) 9 mVLGADAVW (L) 9 mVAAEVGVW m V/L|G\A\D\A\V\W 77.8 7 6 2 1 0 b-ion bias m:Oxidized methionine 77.619774 1.434808 977.47 961.481 15.9888 -6.2 0.0 3.8 6507.4 nuORFdb_human ENST00000452197.5_1_1:173834379-173836179:- ENST00000452197.5_1_1:173834379-173836179:-|ENST00000432536.5_2_1:173835321-173836200:-|ENST00000451607.5_2_1:173835321-173836299:-|ENST00000430245.5_1_1:173835321-173837929:-|ENST00000458220.1_1_1:173835633-173836179:-|ENST00000431268.5_1_1:173835892-173836179:- GAS5 lincRNA lncRNA GN=GAS5 lincRNA E_20221201_NO30_400nL_HLAc1_2e7_directIP_titration_rep1_Slot2-4_1_3515 20mio-rep2 E_20221201_NO30_400nL_HLAc1_2e7_directIP_titration_rep3_Slot2-4_1_3518.22511.476.1 1 5 1 13.23 7.06 4.49 6.17 81.3 7 0 0 34300.0 M1m Hydrogen 1 (-) 9 mVLGADAVW (L) 9 mVAAEVGVW m V/L|G\A\D\A\V\W 77.8 7 6 2 1 0 b-ion bias m:Oxidized methionine 77.754234 1.440403 977.4718 961.481 15.9908 -4.2 2.0999999999999996 3.8 6507.4 nuORFdb_human ENST00000452197.5_1_1:173834379-173836179:- ENST00000452197.5_1_1:173834379-173836179:-|ENST00000432536.5_2_1:173835321-173836200:-|ENST00000451607.5_2_1:173835321-173836299:-|ENST00000430245.5_1_1:173835321-173837929:-|ENST00000458220.1_1_1:173835633-173836179:-|ENST00000431268.5_1_1:173835892-173836179:- GAS5 lincRNA lncRNA GN=GAS5 lincRNA E_20221201_NO30_400nL_HLAc1_2e7_directIP_titration_rep3_Slot2-4_1_3518 20mio-rep3 E_20221201_NO30_400nL_HLAc1_2e7_directIP_titration_rep4_Slot2-4_1_3519.22521.476.1 1 4 1 13.14 6.08 5.92 7.06 79.6 7 0 0 43700.0 M1m Hydrogen 1 (-) 9 mVLGADAVW (L) 9 LNAEVmVW m V/L|G|A\D\A\V\W 77.8 7 6 3 2 0 b-ion bias m:Oxidized methionine 77.780518 1.440515 977.4702 961.481 15.9888 -6.2 -0.40000000000000036 3.8 6507.4 nuORFdb_human ENST00000452197.5_1_1:173834379-173836179:- ENST00000452197.5_1_1:173834379-173836179:-|ENST00000432536.5_2_1:173835321-173836200:-|ENST00000451607.5_2_1:173835321-173836299:-|ENST00000430245.5_1_1:173835321-173837929:-|ENST00000458220.1_1_1:173835633-173836179:-|ENST00000431268.5_1_1:173835892-173836179:- GAS5 lincRNA lncRNA GN=GAS5 lincRNA E_20221201_NO30_400nL_HLAc1_2e7_directIP_titration_rep4_Slot2-4_1_3519 40mio-rep1 E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep1_Slot1-04_1_3496.22772.456.1 1 3 1 16.32 9.01 6.65 7.31 93.7 7 0 0 136000.0 M1m Hydrogen 1 (-) 9 mVLGADAVW (L) 9 LNAEVmVW m V/L|G\A\D\A\V\W 77.8 7 6 2 1 0 b-ion bias m:Oxidized methionine 78.667389 1.452042 977.4727 961.481 15.9918 -3.2 0.0 3.8 6507.4 nuORFdb_human ENST00000452197.5_1_1:173834379-173836179:- ENST00000452197.5_1_1:173834379-173836179:-|ENST00000432536.5_2_1:173835321-173836200:-|ENST00000451607.5_2_1:173835321-173836299:-|ENST00000430245.5_1_1:173835321-173837929:-|ENST00000458220.1_1_1:173835633-173836179:-|ENST00000431268.5_1_1:173835892-173836179:- GAS5 lincRNA lncRNA GN=GAS5 lincRNA E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep1_Slot1-04_1_3496 40mio-rep2 E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep2_Slot2-5_1_3520.22453.473.1 1 4 1 13.99 8.06 4.98 5.92 85.7 7 0 0 83500.0 M1m Hydrogen 1 (-) 9 mVLGADAVW (L) 9 LNAEVmVW m V/L|G\A\D\A\V\W 77.8 7 6 2 1 0 b-ion bias m:Oxidized methionine 77.564964 1.441916 977.4713 961.481 15.9898 -5.2 -0.2999999999999998 3.8 6507.4 nuORFdb_human ENST00000452197.5_1_1:173834379-173836179:- ENST00000452197.5_1_1:173834379-173836179:-|ENST00000432536.5_2_1:173835321-173836200:-|ENST00000451607.5_2_1:173835321-173836299:-|ENST00000430245.5_1_1:173835321-173837929:-|ENST00000458220.1_1_1:173835633-173836179:-|ENST00000431268.5_1_1:173835892-173836179:- GAS5 lincRNA lncRNA GN=GAS5 lincRNA E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep2_Slot2-5_1_3520 40mio-rep3 E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep3_Slot2-5_1_3521.22475.471.1 1 4 1 14.23 8.48 5.06 5.75 85.8 6 0 0 64600.0 M1m Hydrogen 1 (-) 9 mVLGADAVW (L) 9 LNAEVmVW m V/L|G A\D\A\V\W 55.6 5 5 2 1 0 b-ion bias m:Oxidized methionine 77.638924 1.443369 977.4716 961.481 15.9908 -4.2 0.39999999999999947 3.8 6507.4 nuORFdb_human ENST00000452197.5_1_1:173834379-173836179:- ENST00000452197.5_1_1:173834379-173836179:-|ENST00000432536.5_2_1:173835321-173836200:-|ENST00000451607.5_2_1:173835321-173836299:-|ENST00000430245.5_1_1:173835321-173837929:-|ENST00000458220.1_1_1:173835633-173836179:-|ENST00000431268.5_1_1:173835892-173836179:- GAS5 lincRNA lncRNA GN=GAS5 lincRNA E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep3_Slot2-5_1_3521 40mio-rep4 E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep4_Slot2-5_1_3522.22519.471.1 1 4 1 10.57 4.58 5.41 5.99 74.4 7 0 0 66300.0 M1m Hydrogen 1 (-) 9 mVLGADAVW (L) 9 LTMAAGEVAn m V/L|G\A\D\A\V\W 77.8 7 6 2 1 0 b-ion bias m:Oxidized methionine 77.776215 1.443297 977.4744 961.481 15.9928 -2.1 2.6 3.8 6507.4 nuORFdb_human ENST00000452197.5_1_1:173834379-173836179:- ENST00000452197.5_1_1:173834379-173836179:-|ENST00000432536.5_2_1:173835321-173836200:-|ENST00000451607.5_2_1:173835321-173836299:-|ENST00000430245.5_1_1:173835321-173837929:-|ENST00000458220.1_1_1:173835633-173836179:-|ENST00000431268.5_1_1:173835892-173836179:- GAS5 lincRNA lncRNA GN=GAS5 lincRNA E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep4_Slot2-5_1_3522 5mio-rep1 E_20221201_NO30_400nL_HLAc1_5e6_directIP_titration_rep1_Slot1-37_1_3502.22796.478.1 1 2 1 9.48 4.59 4.1 4.89 74.0 7 0 0 13000.0 M1m Hydrogen 1 (-) 9 mVLGADAVW (L) 9 LNAEVmVW m V|L/G\A\D\A\V\W 77.8 7 6 2 1 0 b-ion bias m:Oxidized methionine 78.731796 1.438911 977.4687 961.481 15.9878 -7.2 -0.20000000000000018 3.8 6507.4 nuORFdb_human ENST00000452197.5_1_1:173834379-173836179:- ENST00000452197.5_1_1:173834379-173836179:-|ENST00000432536.5_2_1:173835321-173836200:-|ENST00000451607.5_2_1:173835321-173836299:-|ENST00000430245.5_1_1:173835321-173837929:-|ENST00000458220.1_1_1:173835633-173836179:-|ENST00000431268.5_1_1:173835892-173836179:- GAS5 lincRNA lncRNA GN=GAS5 lincRNA E_20221201_NO30_400nL_HLAc1_5e6_directIP_titration_rep1_Slot1-37_1_3502 5mio-rep3 E_20221201_NO30_400nL_HLAc1_5e6_directIP_titration_rep3_Slot2-2_1_3511.22655.486.1 1 2 1 10.01 4.09 4.84 5.92 73.8 7 0 0 5980.0 M1m Hydrogen 1 (-) 9 mVLGADAVW (L) 9 mVIENARE m V/L|G\A\D\A\V\W 77.8 7 6 2 1 0 b-ion bias m:Oxidized methionine 78.245926 1.434095 977.4708 961.481 15.9898 -5.2 2.999999999999999 3.8 6507.4 nuORFdb_human ENST00000452197.5_1_1:173834379-173836179:- ENST00000452197.5_1_1:173834379-173836179:-|ENST00000432536.5_2_1:173835321-173836200:-|ENST00000451607.5_2_1:173835321-173836299:-|ENST00000430245.5_1_1:173835321-173837929:-|ENST00000458220.1_1_1:173835633-173836179:-|ENST00000431268.5_1_1:173835892-173836179:- GAS5 lincRNA lncRNA GN=GAS5 lincRNA E_20221201_NO30_400nL_HLAc1_5e6_directIP_titration_rep3_Slot2-2_1_3511 SCP E_20221201_NO30_400nL_HLAc1_4e7_PDAC_Slot2-6_1_3524.28163.1208.2 2 1 1 12.55 3.52 9.04 9.04 69.2 7 1 1 20600.0 Hydrogen 3 (R) 11 MWMKCANVWSF (-) 11 PNLHNWSGDWSF M W|M\K C A|N\V|W|S\F 54.5 6 7 4 4 0 dual b/y bias Carbamidomethylation C:Carbamidomethylation, 97.298309 0.995743 730.3181 1459.632 -0.0032 -2.2 0.19999999999999973 8.5 1744.1 nuORFdb_human ENST00000399921.5_1_21:30698443-30698485:+ ENST00000399921.5_1_21:30698443-30698485:+ BACH1 Out-of-Frame Out-of-Frame GN=BACH1 Out-of-Frame E_20221201_NO30_400nL_HLAc1_4e7_PDAC_Slot2-6_1_3524 SCP E_20221201_NO30_400nL_HLAc1_4e7_PDAC_Slot2-6_1_3524.26667.1198.2 2 3 1 18.08 6.61 8.8 11.48 85.5 8 1 1 21000.0 M5m Hydrogen 3 (R) 11 MWmKCANVWSF (-) 11 EGSmGDMWIVTSF M W|m\K\C A|N|V|W|S\F 63.6 7 8 5 5 0 dual b/y bias Carbamidomethylation C:Carbamidomethylation,m:Oxidized methionine 92.128258 1.002206 738.3162 1459.632 15.9928 -1.4 1.0 8.5 1744.1 nuORFdb_human ENST00000399921.5_1_21:30698443-30698485:+ ENST00000399921.5_1_21:30698443-30698485:+ BACH1 Out-of-Frame Out-of-Frame GN=BACH1 Out-of-Frame E_20221201_NO30_400nL_HLAc1_4e7_PDAC_Slot2-6_1_3524 Exploris AL20221201_MKP_PDAC_01.66551.66551.2 2 1 1 14.0 5.87 5.51 8.13 90.9 7 1 1 71100000.0 Hydrogen 1 (-) 11 MYFFRTCLVPI (-) 11 mFGVWEPLLEPL M Y|F/F R\T\C\L\V|P I 45.5 5 6 3 2 0 b-ion bias Carbamidomethylation C:Carbamidomethylation, 0.974 100.0 100.5 40.56 0.0 723.8683 1446.727 0.0021 1.4 0.5999999999999999 8.5 1446.8 nuORFdb_human ENST00000279052.10_1_20:34130319-34130576:+ ENST00000279052.10_1_20:34130319-34130576:+ ERGIC3 ncRNA Retained Intron lncRNA GN=ERGIC3 ncRNA Retained Intron AL20221201_MKP_PDAC_01 Exploris AL20221201_MKP_PDAC_01.65538.65538.2 2 1 1 14.32 6.4 5.54 7.92 81.9 7 1 1 1050000000.0 Hydrogen 12 (Y) 9 MYLKITQII (H) 9 FmIPFLILL M Y|L|K\I\T\Q|I/I 77.8 7 6 4 3 0 dual b/y bias 0.842 82.0 99.08 1.58 0.0 561.8336 1122.659 0.0007 0.6 -0.20000000000000007 8.34 3977.8 nuORFdb_human ENST00000503561.7_1_5:49963943-50055521:+ ENST00000503561.7_1_5:49963943-50055521:+ PARP8 ncRNA Processed Transcript lncRNA GN=PARP8 ncRNA Processed Transcript AL20221201_MKP_PDAC_01 SCP E_20221201_NO30_400nL_HLAc1_4e7_PDAC_Slot2-6_1_3524.28079.192.1 1 1 1 7.44 5.21 7.44 54.2 7 1 1 10400.0 M12m Hydrogen 12 (Y) 9 mYLKITQII (H) 9 m Y/L\K\I\T\Q\I\I 77.8 7 6 1 0 0 b-ion bias m:Oxidized methionine 97.008263 1.607943 1138.6503 1122.659 15.9909 -3.6 -1.2000000000000002 8.34 3977.8 nuORFdb_human ENST00000503561.7_1_5:49963943-50055521:+ ENST00000503561.7_1_5:49963943-50055521:+ PARP8 ncRNA Processed Transcript lncRNA GN=PARP8 ncRNA Processed Transcript E_20221201_NO30_400nL_HLAc1_4e7_PDAC_Slot2-6_1_3524 Exploris_FAIMS VH20221207_MKP_FAIMS2022E_HLAc1_4e7_PDAC.62959.63107.2 2 1 1 7.16 0.9 7.16 6.26 63.2 6 1 1 125000000.0 M12m Hydrogen 12 (Y) 9 mYLKITQII (H) 9 KYmLVKDIL m Y\L\K I\T\Q|I/I 55.6 5 5 2 1 0 b-ion bias m:Oxidized methionine 0.992 81.5 108.03 2.94 -50.0 569.8298 1122.659 15.9931 -1.6 1.0 8.34 3977.8 nuORFdb_human ENST00000503561.7_1_5:49963943-50055521:+ ENST00000503561.7_1_5:49963943-50055521:+ PARP8 ncRNA Processed Transcript lncRNA GN=PARP8 ncRNA Processed Transcript VH20221207_MKP_FAIMS2022E_HLAc1_4e7_PDAC SCP E_20221201_NO30_400nL_HLAc1_4e7_PDAC_Slot2-6_1_3524.14919.1349.2 2 1 1 14.52 11.63 4.04 2.89 88.5 5 1 1 24600.0 Hydrogen 6 (T) 9 MYTRNTVLL (-) 9 MAYWKSVIL M/Y/T R N T\V|L\L 55.6 5 3 3 1 0 unbiased 51.542122 0.908255 555.8001 1110.598 -0.0049 -4.4 -2.0000000000000004 8.5 1729.0 nuORFdb_human ENST00000467790.5_1_1:79116146-79116191:+ ENST00000467790.5_1_1:79116146-79116191:+ IFI44 ncRNA Processed Transcript lncRNA GN=IFI44 ncRNA Processed Transcript E_20221201_NO30_400nL_HLAc1_4e7_PDAC_Slot2-6_1_3524 SCP E_20221201_NO30_400nL_HLAc1_4e7_PDAC_Slot2-6_1_3524.29630.1257.2 2 1 1 14.31 7.7 6.61 6.61 79.0 8 0 0 5130.0 Hydrogen 17 (R) 11 MYVSSQAIFLF (W) 11 GGMLSYASIFLF M Y V\S\S/Q\A|I\F|L\F 72.7 8 7 3 2 0 b-ion bias 102.37027 0.965762 653.3301 1305.655 -0.002 -1.6 0.7999999999999998 5.27 5043.9 nuORFdb_human ENST00000309971.8_1_9:131267149-131271258:+ ENST00000309971.8_1_9:131267149-131271258:+ GLE1 Out-of-Frame Out-of-Frame GN=GLE1 Out-of-Frame E_20221201_NO30_400nL_HLAc1_4e7_PDAC_Slot2-6_1_3524 SCP E_20221201_NO30_400nL_HLAc1_4e7_PDAC_Slot2-6_1_3524.8180.1472.2 2 1 1 11.87 6.48 8.37 5.39 80.3 6 1 1 10500.0 Hydrogen 31 (A) 9 NCSSKVIAM (R) 9 NCSKEKLM N/C|S|S K V\I|A\M 66.7 6 5 4 3 0 dual b/y bias Carbamidomethylation C:Carbamidomethylation, 28.26124 0.831097 505.2423 1009.481 -0.0038 -3.8 -1.4 8.75 7982.2 nuORFdb_human ENST00000590435.5_1_17:74557442-74559369:+ ENST00000590435.5_1_17:74557442-74559369:+ SNHG16 lincRNA lncRNA GN=SNHG16 lincRNA E_20221201_NO30_400nL_HLAc1_4e7_PDAC_Slot2-6_1_3524 20mio-rep2 E_20221201_NO30_400nL_HLAc1_2e7_directIP_titration_rep3_Slot2-4_1_3518.28326.382.1 1 1 1 8.43 4.78 4.55 3.65 73.2 6 0 0 2110.0 Hydrogen 62 (S) 9 NEGGFLLLY (H) 9 DYPKNLIY N E G\G/F/L\L|L\Y 66.7 6 4 3 1 0 unbiased 97.835152 1.496101 1025.5247 1025.53 -0.0102 -10.0 -3.7 4.0 11429.3 nuORFdb_human ENST00000539653.5_1_12:65963787-66035972:- ENST00000539653.5_1_12:65963787-66035972:-|ENST00000546198.5_1_12:65981221-66035915:-|ENST00000535315.5_1_12:65963787-66020413:- RP11-230G5.2 lincRNA lncRNA GN=RP11-230G5.2 lincRNA E_20221201_NO30_400nL_HLAc1_2e7_directIP_titration_rep3_Slot2-4_1_3518 20mio-rep2 E_20221201_NO30_400nL_HLAc1_2e7_directIP_titration_rep3_Slot2-4_1_3518.23017.1422.2 2 1 1 9.7 6.73 3.71 2.97 75.2 6 1 1 2470.0 Hydrogen 20 (L) 9 NEIDLPVRL (P) 9 GGIEDLVRPL N/E|I|D|L|P V R\L 66.7 6 5 5 4 0 dual b/y bias 79.50135 0.862279 534.8051 1068.605 -0.0021 -1.9 4.4 4.37 5686.8 nuORFdb_human ENST00000334318.10_1_9:115038176-115060141:- ENST00000334318.10_1_9:115038176-115060141:- PTBP3 Out-of-Frame Out-of-Frame GN=PTBP3 Out-of-Frame E_20221201_NO30_400nL_HLAc1_2e7_directIP_titration_rep3_Slot2-4_1_3518 20mio-rep3 E_20221201_NO30_400nL_HLAc1_2e7_directIP_titration_rep4_Slot2-4_1_3519.23057.1433.2 2 1 1 9.99 6.15 1.93 3.84 77.7 6 1 1 3660.0 Hydrogen 20 (L) 9 NEIDLPVRL (P) 9 DAGInLIALPA N E|I|D|L|P V/R\L 55.6 5 5 5 4 0 dual b/y bias 79.631233 0.855578 534.804 1068.605 -0.0041 -3.8 2.0 4.37 5686.8 nuORFdb_human ENST00000334318.10_1_9:115038176-115060141:- ENST00000334318.10_1_9:115038176-115060141:- PTBP3 Out-of-Frame Out-of-Frame GN=PTBP3 Out-of-Frame E_20221201_NO30_400nL_HLAc1_2e7_directIP_titration_rep4_Slot2-4_1_3519 40mio-rep1 E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep1_Slot1-04_1_3496.23280.1403.2 2 1 1 14.9 8.35 6.03 6.55 91.6 7 1 1 11900.0 Hydrogen 20 (L) 9 NEIDLPVRL (P) 9 QnLEVPLVR N/E|I|D|L|P/V R\L 77.8 7 5 6 4 0 dual b/y bias 80.422775 0.874765 534.805 1068.605 -0.0021 -1.9 1.3000000000000003 4.37 5686.8 nuORFdb_human ENST00000334318.10_1_9:115038176-115060141:- ENST00000334318.10_1_9:115038176-115060141:- PTBP3 Out-of-Frame Out-of-Frame GN=PTBP3 Out-of-Frame E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep1_Slot1-04_1_3496 40mio-rep2 E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep2_Slot2-5_1_3520.23039.1418.2 2 1 1 13.55 7.69 5.1 5.86 89.3 7 1 1 5510.0 Hydrogen 20 (L) 9 NEIDLPVRL (P) 9 QnLEVPLVR N/E|I|D|L|P/V/R L 77.8 7 4 7 4 0 dual b/y bias 79.589157 0.865058 534.8032 1068.605 -0.0061 -5.7 -0.7999999999999998 4.37 5686.8 nuORFdb_human ENST00000334318.10_1_9:115038176-115060141:- ENST00000334318.10_1_9:115038176-115060141:- PTBP3 Out-of-Frame Out-of-Frame GN=PTBP3 Out-of-Frame E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep2_Slot2-5_1_3520 40mio-rep3 E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep3_Slot2-5_1_3521.23065.1425.2 2 1 1 12.84 8.16 4.23 4.68 89.4 6 1 1 5570.0 Hydrogen 20 (L) 9 NEIDLPVRL (P) 9 GGIEDLVRPL N/E|I|D|L|P V/R L 55.6 5 4 6 4 0 dual b/y bias 79.676277 0.860755 534.8044 1068.605 -0.0041 -3.8 0.7999999999999998 4.37 5686.8 nuORFdb_human ENST00000334318.10_1_9:115038176-115060141:- ENST00000334318.10_1_9:115038176-115060141:- PTBP3 Out-of-Frame Out-of-Frame GN=PTBP3 Out-of-Frame E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep3_Slot2-5_1_3521 40mio-rep4 E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep4_Slot2-5_1_3522.23067.1426.2 2 1 1 10.56 6.92 3.06 3.64 82.2 6 1 1 6040.0 Hydrogen 20 (L) 9 NEIDLPVRL (P) 9 QnLEVPLVR N E|I|D|L|P/V R-L 55.6 5 4 5 4 1 dual b/y bias 79.671043 0.860135 534.8051 1068.605 -0.0021 -1.9 2.8000000000000003 4.37 5686.8 nuORFdb_human ENST00000334318.10_1_9:115038176-115060141:- ENST00000334318.10_1_9:115038176-115060141:- PTBP3 Out-of-Frame Out-of-Frame GN=PTBP3 Out-of-Frame E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep4_Slot2-5_1_3522 40mio-rep1 E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep1_Slot1-04_1_3496.18904.326.1 1 1 1 11.68 4.78 7.47 6.9 82.4 8 0 0 4340.0 Hydrogen 57 (Y) 10 NEVDIGGNIY (T) 10 GLDGLnNLNY N E/V/D|I/G\G\N\I\Y 80.0 8 5 4 1 0 unbiased 65.303841 1.528962 1093.512 1093.516 -0.006 -5.5 -2.3 3.67 8015.1 nuORFdb_human ENST00000555520.1_1_15:93431416-93440966:+ ENST00000555520.1_1_15:93431416-93440966:+|ENST00000557682.6_1_15:93429443-93440969:+ LINC01578 lincRNA lncRNA GN=LINC01578 lincRNA E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep1_Slot1-04_1_3496 SCP E_20221201_NO30_400nL_HLAc1_4e7_PDAC_Slot2-6_1_3524.28825.295.1 1 1 1 8.36 0.75 8.89 7.61 69.3 6 0 0 17900.0 Hydrogen 3 (S) 9 NFIDTALCF (L) 9 nSIFQALCF N F I|D\T\A\L|C-F 66.7 6 5 2 2 1 b-ion bias Carbamidomethylation C:Carbamidomethylation, 99.587044 1.547124 1100.5047 1100.508 -0.0081 -7.4 -5.0 3.8 3204.6 nuORFdb_human ENST00000559350.5_1_15:60678227-60682440:- ENST00000559350.5_1_15:60678227-60682440:- ANXA2 5' Overlap uORF 5' Overlap uORF GN=ANXA2 5' Overlap uORF E_20221201_NO30_400nL_HLAc1_4e7_PDAC_Slot2-6_1_3524 Exploris_FAIMS VH20221207_MKP_FAIMS2022E_HLAc1_4e7_PDAC.38450.38450.2 2 2 1 13.89 13.89 13.89 77.7 7 0 0 29400000.0 Hydrogen 93 (E) 9 NPCAPILHY (R) 9 N/P C|A/P/I/L/H-Y 77.8 7 1 6 1 1 y-ion bias Carbamidomethylation C:Carbamidomethylation, 0.994 99.2 71.38 30.77 -70.0 542.7643 1084.524 -0.0031 -2.9 -0.2999999999999998 6.74 12648.1 nuORFdb_human ENST00000606089.1_1_5:133340855-133341212:+ ENST00000606089.1_1_5:133340855-133341212:+ CTB-113I20.2 lincRNA lncRNA GN=CTB-113I20.2 lincRNA VH20221207_MKP_FAIMS2022E_HLAc1_4e7_PDAC Eva-50mio-3fr AL20210827_EV_HLA_PDAC_PANFRO413_2D_50e6_W632_classI_fxn3.18395.18395.2 2 1 1 8.4 4.12 3.97 4.28 70.5 6 2 2 5130000.0 Hydrogen 30 (A) 10 NPRGARVLAL (-) 10 FRYRKLAL N/P/R/G A R\V L|A/L 50.0 5 2 5 1 0 y-ion bias 0.998 86.6 34.87 100.0 0.0 533.8278 1066.648 0.0004 0.3 -0.2 12.0 3739.1 nuORFdb_human ENST00000338961.10_1_9:130890061-130890178:- ENST00000338961.10_1_9:130890061-130890178:- PTGES2 5' uORF 5' uORF GN=PTGES2 5' uORF AL20210827_EV_HLA_PDAC_PANFRO413_2D_50e6_W632_classI_fxn3 Exploris_FAIMS VH20221207_MKP_FAIMS2022E_HLAc1_4e7_PDAC.27374.27374.2 2 1 1 8.05 6.04 3.28 2.01 65.0 6 2 2 28000000.0 Hydrogen 30 (A) 10 NPRGARVLAL (-) 10 FRYRKLAL N/P R/G A R\V\L/A/L 50.0 5 2 4 0 0 y-ion bias 0.993 78.9 54.9 14.42 -50.0 533.8264 1066.648 -0.0025 -2.4 0.20000000000000018 12.0 3739.1 nuORFdb_human ENST00000338961.10_1_9:130890061-130890178:- ENST00000338961.10_1_9:130890061-130890178:- PTGES2 5' uORF 5' uORF GN=PTGES2 5' uORF VH20221207_MKP_FAIMS2022E_HLAc1_4e7_PDAC Eva-50mio-3fr AL20210827_EV_HLA_PDAC_PANFRO413_2D_50e6_W632_classI_fxn1.14253.14253.2 2 1 1 12.08 3.16 12.08 8.92 79.8 6 0 0 2380000.0 Hydrogen 28 (Q) 9 NSAPEHISY (V) 9 SGGAGGPPGTPY N S\A|P E/H-I/S/Y 55.6 5 2 4 1 1 y-ion bias 0.997 87.7 27.63 100.0 0.0 509.2356 1017.464 0.0003 0.3 0.19999999999999998 5.24 27777.1 nuORFdb_human ENST00000464525.5_1_7:102178866-102207528:- ENST00000464525.5_1_7:102178866-102207528:- POLR2J3 3' Overlap dORF 3' Overlap dORF GN=POLR2J3 3' Overlap dORF AL20210827_EV_HLA_PDAC_PANFRO413_2D_50e6_W632_classI_fxn1 SCP E_20221201_NO30_400nL_HLAc1_4e7_PDAC_Slot2-6_1_3524.8044.1467.2 2 3 1 15.1 7.96 6.51 7.14 83.9 8 0 0 23000.0 Hydrogen 28 (Q) 9 NSAPEHISY (V) 9 NSAmSLEHL N/S/A|P/E/H-I|S\Y 100.0 9 3 6 2 1 y-ion bias 27.791821 0.834075 509.234 1017.464 -0.0029 -2.8 -0.3999999999999999 5.24 27777.1 nuORFdb_human ENST00000464525.5_1_7:102178866-102207528:- ENST00000464525.5_1_7:102178866-102207528:- POLR2J3 3' Overlap dORF 3' Overlap dORF GN=POLR2J3 3' Overlap dORF E_20221201_NO30_400nL_HLAc1_4e7_PDAC_Slot2-6_1_3524 Exploris_FAIMS VH20221207_MKP_FAIMS2022E_HLAc1_4e7_PDAC.22880.22880.2 2 1 1 10.78 1.42 10.78 9.35 76.7 6 0 0 17400000.0 Hydrogen 28 (Q) 9 NSAPEHISY (V) 9 TGNYIHPES N S\A|P E/H-I/S/Y 55.6 5 2 4 1 1 y-ion bias 0.965 86.3 47.53 32.89 -50.0 509.2333 1017.464 -0.0043 -4.2 -1.6 5.24 27777.1 nuORFdb_human ENST00000464525.5_1_7:102178866-102207528:- ENST00000464525.5_1_7:102178866-102207528:- POLR2J3 3' Overlap dORF 3' Overlap dORF GN=POLR2J3 3' Overlap dORF VH20221207_MKP_FAIMS2022E_HLAc1_4e7_PDAC 10mio-rep1 E_20221201_NO30_400nL_HLAc1_1e7_directIP_titration_rep1_Slot2-3_1_3512.13454.1456.2 2 1 1 10.34 6.53 2.98 3.81 79.3 6 0 0 5570.0 Hydrogen 75 (V) 9 NSSDHVLLY (M) 9 PPASnGnLLY N S/S/D H\V\L|L\Y 55.6 5 4 3 1 0 unbiased 46.479969 0.841467 524.2533 1047.511 -0.0118 -11.3 -3.6000000000000005 5.08 11792.1 smORF_v4|nuORFdb_human FBXL4_6:99382638-99374937:- FBXL4_6:99382638-99374937:-|ENST00000369244.6_1_6:99374760-99382655:-|ENST00000229971.2_1_6:99374760-99395723:- FBXL4 overlapping.uORF 6:99382638-99374937:- 6 FBXL4 5' Overlap uORF 5' Overlap uORF GN=FBXL4 E_20221201_NO30_400nL_HLAc1_1e7_directIP_titration_rep1_Slot2-3_1_3512 10mio-rep2 E_20221201_NO30_400nL_HLAc1_1e7_directIP_titration_rep2_Slot2-3_1_3513.13423.1453.2 2 1 1 10.45 6.06 4.39 4.39 80.0 6 0 0 9020.0 Hydrogen 75 (V) 9 NSSDHVLLY (M) 9 FVnDYILY N S/S/D H\V\L|L\Y 55.6 5 4 3 1 0 unbiased 46.372139 0.843226 524.2546 1047.511 -0.0078 -7.5 -0.7999999999999998 5.08 11792.1 smORF_v4|nuORFdb_human FBXL4_6:99382638-99374937:- FBXL4_6:99382638-99374937:-|ENST00000369244.6_1_6:99374760-99382655:-|ENST00000229971.2_1_6:99374760-99395723:- FBXL4 overlapping.uORF 6:99382638-99374937:- 6 FBXL4 5' Overlap uORF 5' Overlap uORF GN=FBXL4 E_20221201_NO30_400nL_HLAc1_1e7_directIP_titration_rep2_Slot2-3_1_3513 10mio-rep3 E_20221201_NO30_400nL_HLAc1_1e7_directIP_titration_rep3_Slot2-3_1_3514.13386.1459.2 2 1 1 13.16 7.06 5.24 6.1 82.3 7 0 0 7520.0 Hydrogen 75 (V) 9 NSSDHVLLY (M) 9 EPTPDGRLY N/S/S/D H\V\L|L\Y 77.8 7 4 4 1 0 unbiased 46.246902 0.839509 524.2542 1047.511 -0.0098 -9.4 -3.1000000000000005 5.08 11792.1 smORF_v4|nuORFdb_human FBXL4_6:99382638-99374937:- FBXL4_6:99382638-99374937:-|ENST00000369244.6_1_6:99374760-99382655:-|ENST00000229971.2_1_6:99374760-99395723:- FBXL4 overlapping.uORF 6:99382638-99374937:- 6 FBXL4 5' Overlap uORF 5' Overlap uORF GN=FBXL4 E_20221201_NO30_400nL_HLAc1_1e7_directIP_titration_rep3_Slot2-3_1_3514 20mio-rep1 E_20221201_NO30_400nL_HLAc1_2e7_directIP_titration_rep1_Slot2-4_1_3515.13347.1449.2 2 1 1 11.69 6.0 4.63 5.68 86.0 6 0 0 15000.0 Hydrogen 75 (V) 9 NSSDHVLLY (M) 9 GQSFGYILY N/S/S D H\V\L|L\Y 66.7 6 4 3 1 0 unbiased 46.099659 0.845856 524.257 1047.511 -0.0038 -3.7 2.5 5.08 11792.1 smORF_v4|nuORFdb_human FBXL4_6:99382638-99374937:- FBXL4_6:99382638-99374937:-|ENST00000369244.6_1_6:99374760-99382655:-|ENST00000229971.2_1_6:99374760-99395723:- FBXL4 overlapping.uORF 6:99382638-99374937:- 6 FBXL4 5' Overlap uORF 5' Overlap uORF GN=FBXL4 E_20221201_NO30_400nL_HLAc1_2e7_directIP_titration_rep1_Slot2-4_1_3515 20mio-rep2 E_20221201_NO30_400nL_HLAc1_2e7_directIP_titration_rep3_Slot2-4_1_3518.13477.1442.2 2 2 1 11.01 6.45 4.23 4.57 78.3 6 0 0 25900.0 Hydrogen 75 (V) 9 NSSDHVLLY (M) 9 SSLFFGNLY N/S/S D H\V/L|L\Y 66.7 6 3 4 1 0 unbiased 46.549549 0.849764 524.2557 1047.511 -0.0058 -5.6 0.7000000000000002 5.08 11792.1 smORF_v4|nuORFdb_human FBXL4_6:99382638-99374937:- FBXL4_6:99382638-99374937:-|ENST00000369244.6_1_6:99374760-99382655:-|ENST00000229971.2_1_6:99374760-99395723:- FBXL4 overlapping.uORF 6:99382638-99374937:- 6 FBXL4 5' Overlap uORF 5' Overlap uORF GN=FBXL4 E_20221201_NO30_400nL_HLAc1_2e7_directIP_titration_rep3_Slot2-4_1_3518 40mio-rep1 E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep1_Slot1-04_1_3496.13708.1418.2 2 2 1 16.91 8.23 8.12 8.68 91.5 8 0 0 69500.0 Hydrogen 75 (V) 9 NSSDHVLLY (M) 9 GQSFGYILY N/S/S/D/H\V|L|L\Y 100.0 9 4 6 2 0 unbiased 47.351692 0.864867 524.2578 1047.511 -0.0018 -1.7 1.5000000000000002 5.08 11792.1 smORF_v4|nuORFdb_human FBXL4_6:99382638-99374937:- FBXL4_6:99382638-99374937:-|ENST00000369244.6_1_6:99374760-99382655:-|ENST00000229971.2_1_6:99374760-99395723:- FBXL4 overlapping.uORF 6:99382638-99374937:- 6 FBXL4 5' Overlap uORF 5' Overlap uORF GN=FBXL4 E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep1_Slot1-04_1_3496 40mio-rep2 E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep2_Slot2-5_1_3520.13346.1429.2 2 2 1 16.52 9.09 5.78 7.43 90.8 8 0 0 64000.0 Hydrogen 75 (V) 9 NSSDHVLLY (M) 9 GQSFGYILY N/S/S/D/H\V|L|L\Y 100.0 9 4 6 2 0 unbiased 46.103199 0.858095 524.2568 1047.511 -0.0038 -3.7 1.2000000000000002 5.08 11792.1 smORF_v4|nuORFdb_human FBXL4_6:99382638-99374937:- FBXL4_6:99382638-99374937:-|ENST00000369244.6_1_6:99374760-99382655:-|ENST00000229971.2_1_6:99374760-99395723:- FBXL4 overlapping.uORF 6:99382638-99374937:- 6 FBXL4 5' Overlap uORF 5' Overlap uORF GN=FBXL4 E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep2_Slot2-5_1_3520 40mio-rep3 E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep3_Slot2-5_1_3521.13334.1434.2 2 2 1 13.03 7.02 4.94 6.01 86.5 6 0 0 42900.0 Hydrogen 75 (V) 9 NSSDHVLLY (M) 9 GQSFGYILY N/S/S D H\V\L|L\Y 66.7 6 4 3 1 0 unbiased 46.066216 0.855209 524.2571 1047.511 -0.0038 -3.7 0.8999999999999995 5.08 11792.1 smORF_v4|nuORFdb_human FBXL4_6:99382638-99374937:- FBXL4_6:99382638-99374937:-|ENST00000369244.6_1_6:99374760-99382655:-|ENST00000229971.2_1_6:99374760-99395723:- FBXL4 overlapping.uORF 6:99382638-99374937:- 6 FBXL4 5' Overlap uORF 5' Overlap uORF GN=FBXL4 E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep3_Slot2-5_1_3521 40mio-rep4 E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep4_Slot2-5_1_3522.13334.1432.2 2 2 1 14.13 6.97 7.09 7.16 83.9 7 0 0 43600.0 Hydrogen 75 (V) 9 NSSDHVLLY (M) 9 EPTPDGRLY N/S/S/D H\V\L|L\Y 77.8 7 4 4 1 0 unbiased 46.047176 0.856066 524.2565 1047.511 -0.0058 -5.6 -0.8999999999999995 5.08 11792.1 smORF_v4|nuORFdb_human FBXL4_6:99382638-99374937:- FBXL4_6:99382638-99374937:-|ENST00000369244.6_1_6:99374760-99382655:-|ENST00000229971.2_1_6:99374760-99395723:- FBXL4 overlapping.uORF 6:99382638-99374937:- 6 FBXL4 5' Overlap uORF 5' Overlap uORF GN=FBXL4 E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep4_Slot2-5_1_3522 5mio-rep1 E_20221201_NO30_400nL_HLAc1_5e6_directIP_titration_rep1_Slot1-37_1_3502.13738.1443.2 2 1 1 11.75 7.02 4.05 4.74 81.8 6 0 0 3980.0 Hydrogen 75 (V) 9 NSSDHVLLY (M) 9 MVDRHNLY N/S/S D H\V\L|L\Y 66.7 6 4 3 1 0 unbiased 47.454288 0.849557 524.2562 1047.511 -0.0058 -5.6 1.4000000000000004 5.08 11792.1 smORF_v4|nuORFdb_human FBXL4_6:99382638-99374937:- FBXL4_6:99382638-99374937:-|ENST00000369244.6_1_6:99374760-99382655:-|ENST00000229971.2_1_6:99374760-99395723:- FBXL4 overlapping.uORF 6:99382638-99374937:- 6 FBXL4 5' Overlap uORF 5' Overlap uORF GN=FBXL4 E_20221201_NO30_400nL_HLAc1_5e6_directIP_titration_rep1_Slot1-37_1_3502 5mio-rep2 E_20221201_NO30_400nL_HLAc1_5e6_directIP_titration_rep2_Slot2-2_1_3510.13651.1453.2 2 2 1 9.78 5.92 3.42 3.86 77.1 5 0 0 9460.0 Hydrogen 75 (V) 9 NSSDHVLLY (M) 9 IEPYYSIY N/S/S D H V\L|L\Y 55.6 5 3 3 1 0 unbiased 47.149075 0.843229 524.2536 1047.511 -0.0098 -9.4 -0.7000000000000011 5.08 11792.1 smORF_v4|nuORFdb_human FBXL4_6:99382638-99374937:- FBXL4_6:99382638-99374937:-|ENST00000369244.6_1_6:99374760-99382655:-|ENST00000229971.2_1_6:99374760-99395723:- FBXL4 overlapping.uORF 6:99382638-99374937:- 6 FBXL4 5' Overlap uORF 5' Overlap uORF GN=FBXL4 E_20221201_NO30_400nL_HLAc1_5e6_directIP_titration_rep2_Slot2-2_1_3510 40mio-rep1 E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep1_Slot1-04_1_3496.15125.1275.2 2 2 1 10.42 10.42 10.42 72.2 8 1 1 12200.0 Hydrogen 3 (T) 9 NSWMMARHW (A) 9 N/S/W|M/M/A/R|H\W 100.0 9 3 7 2 0 y-ion bias 52.24593 0.954665 609.7671 1218.53 -0.0028 -2.3 0.9000000000000004 9.75 6388.3 nuORFdb_human ENST00000494946.6_1_16:2822040-2825556:- ENST00000494946.6_1_16:2822040-2825556:- ELOB Out-of-Frame Out-of-Frame GN=ELOB Out-of-Frame E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep1_Slot1-04_1_3496 40mio-rep1 E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep1_Slot1-04_1_3496.8958.1304.2 2 1 1 8.69 8.69 8.69 59.9 7 1 1 7380.0 M6m M7m Hydrogen 3 (T) 9 NSWmmARHW (A) 9 N/S/W/m|m A/R|H\W 77.8 7 3 6 2 0 y-ion bias m:Oxidized methionine 30.948322 0.936163 625.7627 1218.53 31.9892 -0.5 2.7 9.75 6388.3 nuORFdb_human ENST00000494946.6_1_16:2822040-2825556:- ENST00000494946.6_1_16:2822040-2825556:- ELOB Out-of-Frame Out-of-Frame GN=ELOB Out-of-Frame E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep1_Slot1-04_1_3496 20mio-rep2 E_20221201_NO30_400nL_HLAc1_2e7_directIP_titration_rep3_Slot2-4_1_3518.8619.1450.2 2 1 1 9.3 4.01 5.43 5.29 74.8 8 0 1 3730.0 Hydrogen 37 (V) 10 NTESFGTGIK (-) 10 SQEKYWGR N/T/E|S/F/G/T/G I\K 80.0 8 2 7 1 0 y-ion bias 29.775238 0.844832 527.2625 1053.521 -0.0024 -2.3 4.0 6.0 4690.1 nuORFdb_human ENST00000381118.7_1_5:60224765-60240875:- ENST00000381118.7_1_5:60224765-60240875:- ERCC8 5' Overlap uORF 5' Overlap uORF GN=ERCC8 5' Overlap uORF E_20221201_NO30_400nL_HLAc1_2e7_directIP_titration_rep3_Slot2-4_1_3518 40mio-rep2 E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep2_Slot2-5_1_3520.8473.1434.2 2 2 1 12.28 7.07 5.83 5.21 81.3 6 0 1 17400.0 Hydrogen 37 (V) 10 NTESFGTGIK (-) 10 nSAAAKYLTN N/T|E|S|F/G T G-I\K 70.0 7 4 5 3 1 unbiased 29.273338 0.85507 527.2614 1053.521 -0.0064 -6.1 -1.1999999999999993 6.0 4690.1 nuORFdb_human ENST00000381118.7_1_5:60224765-60240875:- ENST00000381118.7_1_5:60224765-60240875:- ERCC8 5' Overlap uORF 5' Overlap uORF GN=ERCC8 5' Overlap uORF E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep2_Slot2-5_1_3520 40mio-rep3 E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep3_Slot2-5_1_3521.8486.1440.2 2 1 1 7.26 4.57 2.96 2.69 70.8 6 0 1 12300.0 Hydrogen 37 (V) 10 NTESFGTGIK (-) 10 SQEFSKLQS N/T/E|S/F/G/T G I K 60.0 6 1 6 1 0 y-ion bias 29.317039 0.851198 527.2623 1053.521 -0.0044 -4.2 0.39999999999999947 6.0 4690.1 nuORFdb_human ENST00000381118.7_1_5:60224765-60240875:- ENST00000381118.7_1_5:60224765-60240875:- ERCC8 5' Overlap uORF 5' Overlap uORF GN=ERCC8 5' Overlap uORF E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep3_Slot2-5_1_3521 40mio-rep4 E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep4_Slot2-5_1_3522.8487.1440.2 2 1 1 9.16 5.15 4.02 4.01 68.8 7 0 1 10100.0 Hydrogen 37 (V) 10 NTESFGTGIK (-) 10 SQEFSKLQS N/T|E/S|F/G/T/G-I K 80.0 8 2 7 2 1 y-ion bias 29.313841 0.851311 527.2613 1053.521 -0.0064 -6.1 -1.3999999999999995 6.0 4690.1 nuORFdb_human ENST00000381118.7_1_5:60224765-60240875:- ENST00000381118.7_1_5:60224765-60240875:- ERCC8 5' Overlap uORF 5' Overlap uORF GN=ERCC8 5' Overlap uORF E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep4_Slot2-5_1_3522 20mio-rep1 E_20221201_NO30_400nL_HLAc1_2e7_directIP_titration_rep1_Slot2-4_1_3515.8707.1485.2 2 1 1 9.16 3.72 5.23 5.44 64.6 7 1 1 5770.0 Hydrogen 22 (K) 9 NTSSVLRSY (L) 9 SAGAMALRYS N/T/S/S|V/L/R|S Y 77.8 7 2 7 2 0 y-ion bias 30.07782 0.822935 513.7602 1026.521 -0.0087 -8.5 -2.3 8.75 3784.5 nuORFdb_human ENST00000456324.5_1_19:36726621-36726717:+ ENST00000456324.5_1_19:36726621-36726717:+ ZNF146 5' uORF 5' uORF GN=ZNF146 5' uORF E_20221201_NO30_400nL_HLAc1_2e7_directIP_titration_rep1_Slot2-4_1_3515 20mio-rep2 E_20221201_NO30_400nL_HLAc1_2e7_directIP_titration_rep3_Slot2-4_1_3518.8877.1482.2 2 1 1 8.38 2.37 6.5 6.02 64.4 7 1 1 4420.0 Hydrogen 22 (K) 9 NTSSVLRSY (L) 9 NEVLIHSSQ N/T/S/S V/L|R\S\Y 77.8 7 3 5 1 0 unbiased 30.665705 0.825 513.7611 1026.521 -0.0067 -6.6 -0.2999999999999998 8.75 3784.5 nuORFdb_human ENST00000456324.5_1_19:36726621-36726717:+ ENST00000456324.5_1_19:36726621-36726717:+ ZNF146 5' uORF 5' uORF GN=ZNF146 5' uORF E_20221201_NO30_400nL_HLAc1_2e7_directIP_titration_rep3_Slot2-4_1_3518 40mio-rep1 E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep1_Slot1-04_1_3496.8976.1453.2 2 1 1 13.67 5.97 8.32 7.7 85.1 8 1 1 8470.0 Hydrogen 22 (K) 9 NTSSVLRSY (L) 9 SGASSHLLSPA N/T/S|S/V/L/R|S-Y 100.0 9 2 7 2 1 y-ion bias 31.010462 0.84341 513.7638 1026.521 -0.0007 -0.7 2.5 8.75 3784.5 nuORFdb_human ENST00000456324.5_1_19:36726621-36726717:+ ENST00000456324.5_1_19:36726621-36726717:+ ZNF146 5' uORF 5' uORF GN=ZNF146 5' uORF E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep1_Slot1-04_1_3496 40mio-rep2 E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep2_Slot2-5_1_3520.8707.1476.2 2 1 1 10.85 3.72 6.79 7.13 68.6 7 1 1 9680.0 Hydrogen 22 (K) 9 NTSSVLRSY (L) 9 GTGSSVEKYV N/T|S/S/V|L|R|S Y 77.8 7 4 7 4 0 dual b/y bias 30.081181 0.828379 513.7617 1026.521 -0.0047 -4.6 0.3000000000000007 8.75 3784.5 nuORFdb_human ENST00000456324.5_1_19:36726621-36726717:+ ENST00000456324.5_1_19:36726621-36726717:+ ZNF146 5' uORF 5' uORF GN=ZNF146 5' uORF E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep2_Slot2-5_1_3520 40mio-rep3 E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep3_Slot2-5_1_3521.8716.1476.2 2 1 1 8.73 3.15 6.94 5.58 62.1 6 1 1 12500.0 Hydrogen 22 (K) 9 NTSSVLRSY (L) 9 SGASSHLLSPA N T/S|S/V|L|R S\Y 55.6 5 4 5 3 0 dual b/y bias 30.110872 0.828532 513.7616 1026.521 -0.0047 -4.6 0.0 8.75 3784.5 nuORFdb_human ENST00000456324.5_1_19:36726621-36726717:+ ENST00000456324.5_1_19:36726621-36726717:+ ZNF146 5' uORF 5' uORF GN=ZNF146 5' uORF E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep3_Slot2-5_1_3521 40mio-rep4 E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep4_Slot2-5_1_3522.8718.1471.2 2 1 1 8.02 4.32 3.41 3.7 70.3 6 1 1 8010.0 Hydrogen 22 (K) 9 NTSSVLRSY (L) 9 GTGSSVEKYV N T|S/S/V/L/R|S Y 55.6 5 2 6 2 0 y-ion bias 30.111397 0.831808 513.7613 1026.521 -0.0067 -6.6 -1.8999999999999995 8.75 3784.5 nuORFdb_human ENST00000456324.5_1_19:36726621-36726717:+ ENST00000456324.5_1_19:36726621-36726717:+ ZNF146 5' uORF 5' uORF GN=ZNF146 5' uORF E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep4_Slot2-5_1_3522 Eva-50mio-3fr AL20210827_EV_HLA_PDAC_PANFRO413_2D_50e6_W632_classI_fxn3.54276.54276.2 2 1 1 12.9 -1.25 12.9 14.15 84.2 7 0 0 13500000.0 Hydrogen 10 (V) 11 NWFPASENLVF (H) 11 SQVSNWFGnKR N W\F\P A\S E\N\L|V/F 45.5 5 6 2 1 0 b-ion bias 0.992 90.3 97.67 69.21 0.0 662.3226 1323.637 0.0012 0.9 0.4 4.0 3701.1 nuORFdb_human ENST00000265713.6_2_8:41906525-41906624:- ENST00000265713.6_2_8:41906525-41906624:- KAT6A 5' uORF 5' uORF GN=KAT6A 5' uORF AL20210827_EV_HLA_PDAC_PANFRO413_2D_50e6_W632_classI_fxn3 Exploris AL20221201_MKP_PDAC_01.67108.67108.2 2 1 1 14.82 -2.71 14.82 17.53 96.1 7 0 0 285000000.0 Hydrogen 10 (V) 11 NWFPASENLVF (H) 11 SQVSNWFGnKR N W\F\P A\S E\N\L|V/F 45.5 5 6 2 1 0 b-ion bias 0.998 100.0 100.4 42.5 0.0 662.3227 1323.637 0.0013 1.0 0.19999999999999996 4.0 3701.1 nuORFdb_human ENST00000265713.6_2_8:41906525-41906624:- ENST00000265713.6_2_8:41906525-41906624:- KAT6A 5' uORF 5' uORF GN=KAT6A 5' uORF AL20221201_MKP_PDAC_01 SCP E_20221201_NO30_400nL_HLAc1_4e7_PDAC_Slot2-6_1_3524.29266.70.1 1 1 1 15.36 3.46 10.87 11.9 95.3 9 0 0 9500.0 Hydrogen 10 (V) 11 NWFPASENLVF (H) 11 VARECLSLnNF N/W/F|P A\S\E\N\L\V\F 81.8 9 7 3 1 0 b-ion bias 101.112801 1.679061 1323.6337 1323.637 -0.0068 -5.1 -2.6999999999999997 4.0 3701.1 nuORFdb_human ENST00000265713.6_2_8:41906525-41906624:- ENST00000265713.6_2_8:41906525-41906624:- KAT6A 5' uORF 5' uORF GN=KAT6A 5' uORF E_20221201_NO30_400nL_HLAc1_4e7_PDAC_Slot2-6_1_3524 SCP E_20221201_NO30_400nL_HLAc1_4e7_PDAC_Slot2-6_1_3524.29429.261.1 1 1 1 11.46 6.46 4.16 5.0 74.5 7 0 0 46200.0 Hydrogen 63 (T) 9 NYIISVLNL (L) 9 YVAGSVILNI N Y\I|I|S\V\L\N\L 77.8 7 7 2 2 0 b-ion bias 101.676247 1.567389 1048.6039 1048.604 -0.0037 -3.6 -1.2000000000000002 5.52 22214.5 nuORFdb_human ENST00000566127.1_1_16:29556504-29560080:- ENST00000566127.1_1_16:29556504-29560080:-|ENST00000529428.5_1_16:30296511-30300103:- SMG1P2 Pseudogene Pseudogene GN=SMG1P2 Pseudogene E_20221201_NO30_400nL_HLAc1_4e7_PDAC_Slot2-6_1_3524 SCP E_20221201_NO30_400nL_HLAc1_4e7_PDAC_Slot2-6_1_3524.26145.1299.2 2 1 1 12.31 2.59 12.7 9.72 73.6 8 1 2 8520.0 Hydrogen 34 (L) 9 NYIKKIMTI (S) 9 LNYIKKIMT N/Y|I|K/K\I|M|T-I 100.0 9 5 6 4 1 dual b/y bias 90.32251 0.939277 562.3298 1123.654 -0.0017 -1.5 0.8999999999999999 9.7 5161.1 nuORFdb_human ENST00000371621.4_1_20:49177909-49181565:+ ENST00000371621.4_1_20:49177909-49181565:+ PTPN1 Out-of-Frame Out-of-Frame GN=PTPN1 Out-of-Frame E_20221201_NO30_400nL_HLAc1_4e7_PDAC_Slot2-6_1_3524 SCP E_20221201_NO30_400nL_HLAc1_4e7_PDAC_Slot2-6_1_3524.25336.1376.2 2 16 1 17.22 8.32 7.91 8.9 84.8 8 0 0 1520000.0 Hydrogen 2 (L) 9 NYQGHFHLF (-) 9 NVSSSSNAHLF N/Y\Q|G|H\F|H|L\F 100.0 9 7 5 4 0 dual b/y bias 87.528107 0.891342 581.7734 1162.543 -0.0041 -3.6 -1.2000000000000002 6.92 1275.4 nuORFdb_human ENST00000283632.4_1_2:86968058-86968091:+ ENST00000283632.4_1_2:86968058-86968091:+ RMND5A Out-of-Frame Out-of-Frame GN=RMND5A Out-of-Frame E_20221201_NO30_400nL_HLAc1_4e7_PDAC_Slot2-6_1_3524 SCP E_20221201_NO30_400nL_HLAc1_4e7_PDAC_Slot2-6_1_3524.10057.1267.2 2 2 1 11.09 5.53 5.33 5.56 71.7 8 2 2 22500.0 Hydrogen 39 (R) 9 NYTEIREKL (R) 9 LKELRETYN N/Y|T/E/I/R-E\K-L 100.0 9 2 5 1 2 y-ion bias 34.745358 0.959648 583.3128 1165.621 -0.0024 -2.1 0.2999999999999998 6.14 55543.3 nuORFdb_human ENST00000510479.5_1_5:176948978-176959043:- ENST00000510479.5_1_5:176948978-176959043:- FAM193B 3' Overlap dORF 3' Overlap dORF GN=FAM193B 3' Overlap dORF E_20221201_NO30_400nL_HLAc1_4e7_PDAC_Slot2-6_1_3524 Exploris_FAIMS VH20221207_MKP_FAIMS2022E_HLAc1_4e7_PDAC.58942.59179.2 2 1 1 9.46 9.46 9.46 59.8 7 1 1 13700000.0 Hydrogen 4 (R) 9 NYYKVNWTF (L) 9 N Y|Y\K-V\N\W/T/F 77.8 7 4 3 1 1 unbiased 0.976 95.2 102.8 117.65 -50.0 617.7968 1234.589 -0.0029 -2.4 0.20000000000000018 8.5 2359.8 nuORFdb_human ENST00000518345.1_1_8:109254078-109254135:- ENST00000518345.1_1_8:109254078-109254135:- EIF3E 5' Overlap uORF 5' Overlap uORF GN=EIF3E 5' Overlap uORF VH20221207_MKP_FAIMS2022E_HLAc1_4e7_PDAC SCP E_20221201_NO30_400nL_HLAc1_4e7_PDAC_Slot2-6_1_3524.7462.1442.2 2 1 1 8.58 3.49 4.29 5.09 77.0 6 2 2 14000.0 Hydrogen 55 (L) 8 PASNRIKY (-) 8 PALPPPPPY P/A/S/N R\I\K\Y 75.0 6 3 3 0 0 unbiased 25.782175 0.84979 474.7653 948.526 -0.0034 -3.6 -1.2000000000000002 10.01 7124.1 nuORFdb_human ENST00000511208.2_1_5:31532460-31535865:+ ENST00000511208.2_1_5:31532460-31535865:+ C5orf22 Nonsense Mediated Decay Other GN=C5orf22 Nonsense Mediated Decay E_20221201_NO30_400nL_HLAc1_4e7_PDAC_Slot2-6_1_3524 SCP E_20221201_NO30_400nL_HLAc1_4e7_PDAC_Slot2-6_1_3524.26997.1458.2 2 1 1 9.88 9.88 9.88 50.8 7 0 0 50400.0 Hydrogen 2 (M) 8 PFLHLHNM (S) 8 P/F/L|H/L/H|N\M 100.0 8 3 6 2 0 y-ion bias 93.267731 0.839791 504.7569 1008.508 -0.0017 -1.6 0.7999999999999998 7.33 3266.8 smORF_v4|nuORFdb_human PCSK7_11:117101066-117100572:- PCSK7_11:117101066-117100572:-|ENST00000532301.5_1_11:117100562-117101901:-|ENST00000524507.5_1_11:117100562-117102552:-|ENST00000320934.7_1_11:117100562-117103224:- PCSK7 uORF 11:117101066-117100572:- 11 PCSK7 5' uORF 5' uORF GN=PCSK7 E_20221201_NO30_400nL_HLAc1_4e7_PDAC_Slot2-6_1_3524 SCP E_20221201_NO30_400nL_HLAc1_4e7_PDAC_Slot2-6_1_3524.16357.1378.2 2 2 1 16.83 4.75 10.73 12.08 83.3 8 0 0 15000.0 Hydrogen 36 (M) 9 PFVHVVYAH (-) 9 FPVHQARDV P/F|V|H|V|V|Y\A\H 100.0 9 7 6 5 0 dual b/y bias 56.509094 0.890067 534.7831 1068.562 -0.0038 -3.6 -1.2000000000000002 7.32 4834.4 nuORFdb_human ENST00000602122.5_1_19:35739636-35739768:+ ENST00000602122.5_1_19:35739636-35739768:+ LSR 5' uORF 5' uORF GN=LSR 5' uORF E_20221201_NO30_400nL_HLAc1_4e7_PDAC_Slot2-6_1_3524 SCP E_20221201_NO30_400nL_HLAc1_4e7_PDAC_Slot2-6_1_3524.13917.1470.2 2 1 1 15.84 8.04 7.0 7.81 85.2 7 1 1 75900.0 Hydrogen 818 (L) 8 PHFIKDDY (G) 8 PHYTPSVAY P/H|F|I/K\D|D\Y 100.0 8 5 5 3 0 dual b/y bias 48.078934 0.832399 517.7489 1034.494 -0.0034 -3.3 -0.8999999999999999 5.22 213870.4 nuORFdb_human ENST00000617998.4_2_17:7388053-7417318:+ ENST00000617998.4_2_17:7388053-7417318:+ POLR2A 5' Overlap uORF 5' Overlap uORF GN=POLR2A 5' Overlap uORF E_20221201_NO30_400nL_HLAc1_4e7_PDAC_Slot2-6_1_3524 SCP E_20221201_NO30_400nL_HLAc1_4e7_PDAC_Slot2-6_1_3524.10946.1534.2 2 3 1 9.46 5.65 2.33 3.81 66.7 7 1 1 21500.0 Hydrogen 100 (F) 8 PHRLPPSY (V) 8 PHIPRSPY P/H/R\L\P|P\S\Y 100.0 8 5 3 1 0 unbiased 37.816593 0.79193 483.7586 966.516 -0.0049 -5.0 -2.6 9.18 14634.5 nuORFdb_human ENST00000598164.2_1_19:40172096-40174784:+ ENST00000598164.2_1_19:40172096-40174784:+ LGALS17A Pseudogene Pseudogene GN=LGALS17A Pseudogene E_20221201_NO30_400nL_HLAc1_4e7_PDAC_Slot2-6_1_3524 SCP E_20221201_NO30_400nL_HLAc1_4e7_PDAC_Slot2-6_1_3524.7261.1513.2 2 3 1 12.42 8.67 2.13 3.75 83.7 6 0 1 40800.0 Hydrogen 7 (V) 8 PHTRPVSL (S) 8 PHTPVRTV P/H|T/R\P-V\S\L 100.0 8 4 3 1 1 unbiased 25.088453 0.805261 453.7603 906.516 -0.0029 -3.1 -0.7000000000000002 10.18 17668.9 nuORFdb_human ENST00000412609.5_1_19:40170059-40174276:+ ENST00000412609.5_1_19:40170059-40174276:+ LGALS17A Pseudogene Pseudogene GN=LGALS17A Pseudogene E_20221201_NO30_400nL_HLAc1_4e7_PDAC_Slot2-6_1_3524 Eva-50mio-3fr AL20210827_EV_HLA_PDAC_PANFRO413_2D_50e6_W632_classI_fxn3.25128.25128.2 2 1 1 12.78 11.43 0.32 1.35 80.8 5 1 1 6210000.0 Hydrogen 95 (K) 9 PISSRATLI (L) 9 LPSTRLGTL P I|S/S R A\T\L|I 44.4 4 4 3 2 0 unbiased 0.998 94.1 46.72 100.0 0.0 479.2904 957.573 0.0007 0.7 0.19999999999999996 10.18 12146.4 nuORFdb_human ENST00000483662.2_1_11:70266591-70267883:+ ENST00000483662.2_1_11:70266591-70267883:+ CTTN ncRNA Retained Intron lncRNA GN=CTTN ncRNA Retained Intron AL20210827_EV_HLA_PDAC_PANFRO413_2D_50e6_W632_classI_fxn3 SCP E_20221201_NO30_400nL_HLAc1_4e7_PDAC_Slot2-6_1_3524.10626.444.1 1 1 1 11.74 5.64 5.99 6.1 76.3 7 1 1 3980.0 Hydrogen 23 (A) 9 PKSSSGFSL (-) 9 RWSGGFSL P/K/S S\S\G|F\S\L 77.8 7 5 3 1 0 unbiased 36.710915 1.459328 909.4657 909.468 -0.0016 -1.8 0.5999999999999999 9.18 3409.0 nuORFdb_human ENST00000565420.5_1_16:18800314-18800410:- ENST00000565420.5_1_16:18800314-18800410:- RPS15A Out-of-Frame Out-of-Frame GN=RPS15A Out-of-Frame E_20221201_NO30_400nL_HLAc1_4e7_PDAC_Slot2-6_1_3524 SCP E_20221201_NO30_400nL_HLAc1_4e7_PDAC_Slot2-6_1_3524.7700.1421.2 2 1 1 14.06 4.17 9.32 9.89 84.6 7 0 0 6310.0 Hydrogen 48 (L) 8 PLAHHHLY (F) 8 PLPSSNPYL P/L/A/H|H|H|L\Y 100.0 8 4 6 3 0 dual b/y bias 26.603678 0.863429 494.2615 987.516 -0.0012 -1.2 1.2 7.42 8586.7 nuORFdb_human ENST00000406386.7_1_22:38136963-38150984:+ ENST00000406386.7_1_22:38136963-38150984:+|ENST00000407319.6_1_22:38142252-38150984:+|ENST00000413051.2_1_22:38146679-38150984:+|ENST00000418339.5_2_22:38147125-38150984:+|ENST00000452519.5_2_22:38147480-38150984:+|ENST00000417857.1_2_22:38147480-38150984:+ TRIOBP Out-of-Frame Out-of-Frame GN=TRIOBP Out-of-Frame E_20221201_NO30_400nL_HLAc1_4e7_PDAC_Slot2-6_1_3524 SCP E_20221201_NO30_400nL_HLAc1_4e7_PDAC_Slot2-6_1_3524.30249.491.1 1 2 1 14.35 9.29 5.76 5.06 93.7 8 0 0 47000.0 Hydrogen 46 (Y) 9 PPLLGALSF (I) 9 PPLLQLFS P/P/L|L\G\A\L\S\F 100.0 9 6 3 1 0 b-ion bias 104.508286 1.431364 914.5329 914.535 -0.0016 -1.7 0.7 5.96 6097.9 smORF_v4|nuORFdb_human MLLT3_9:20622328-20622507:- MLLT3_9:20622328-20622507:-|ENST00000380338.8_1_9:20622330-20622507:- MLLT3 uORF 9:20622328-20622507:- 9 MLLT3 5' uORF 5' uORF GN=MLLT3 E_20221201_NO30_400nL_HLAc1_4e7_PDAC_Slot2-6_1_3524 SCP E_20221201_NO30_400nL_HLAc1_4e7_PDAC_Slot2-6_1_3524.4358.1501.2 2 1 1 12.3 9.89 2.06 2.41 82.3 5 1 2 649000.0 Hydrogen 18 (A) 8 PRASRPSL (-) 8 RPSARPSI P/R|A/S R|P-S\L 75.0 6 3 4 2 1 unbiased 15.0659 0.813092 442.2555 883.511 -0.0061 -6.9 -4.5 12.0 2896.3 nuORFdb_human ENST00000310441.11_1_X:153236587-153236665:- ENST00000310441.11_1_X:153236587-153236665:- HCFC1 5' uORF 5' uORF GN=HCFC1 5' uORF E_20221201_NO30_400nL_HLAc1_4e7_PDAC_Slot2-6_1_3524 SCP E_20221201_NO30_400nL_HLAc1_4e7_PDAC_Slot2-6_1_3524.8312.1454.2 2 4 1 12.98 6.6 6.24 6.39 89.6 6 0 2 38800.0 Hydrogen 16 (L) 8 PRPPGSLK (-) 8 PPPGRSLK P/R|P|P-G\S|L\K 100.0 8 5 4 3 1 dual b/y bias 28.716808 0.842395 426.2573 851.51 -0.003 -3.6 -1.2000000000000002 11.01 2438.8 nuORFdb_human ENST00000598273.5_1_19:48284145-48284407:+ ENST00000598273.5_1_19:48284145-48284407:+ SELENOW ncRNA Retained Intron lncRNA GN=SELENOW ncRNA Retained Intron E_20221201_NO30_400nL_HLAc1_4e7_PDAC_Slot2-6_1_3524 SCP E_20221201_NO30_400nL_HLAc1_4e7_PDAC_Slot2-6_1_3524.12558.1447.2 2 2 1 13.45 6.52 5.56 6.93 86.8 6 1 2 30800.0 Hydrogen 5 (M) 8 PSKKLILY (L) 8 LQALLKIY P/S/K|K L\I|L\Y 75.0 6 4 4 2 0 unbiased 43.383347 0.846957 481.306 961.608 -0.0034 -3.5 -1.1 9.72 1823.4 nuORFdb_human ENST00000316423.10_1_4:39512477-39515746:- ENST00000316423.10_1_4:39512477-39515746:- UGDH Out-of-Frame Out-of-Frame GN=UGDH Out-of-Frame E_20221201_NO30_400nL_HLAc1_4e7_PDAC_Slot2-6_1_3524 Eva-50mio-3fr AL20210827_EV_HLA_PDAC_PANFRO413_2D_50e6_W632_classI_fxn1.35308.35409.2 2 1 1 8.16 6.05 2.52 2.11 55.3 6 0 0 45100000.0 Hydrogen 95 (A) 10 PVPEFIDPAF (P) 10 YVTVGEFVAF P V P\E\F\I\D P/A/F 50.0 5 4 2 0 0 b-ion bias -21305.197 85.7 63.32 100.0 0.0 566.2906 1131.572 0.0018 1.6 1.5 3.66 12858.8 nuORFdb_human ENST00000612792.1_1_16:25269551-25269908:+ ENST00000612792.1_1_16:25269551-25269908:+ CTD-2547G23.4 lincRNA lncRNA GN=CTD-2547G23.4 lincRNA AL20210827_EV_HLA_PDAC_PANFRO413_2D_50e6_W632_classI_fxn1 10mio-rep1 E_20221201_NO30_400nL_HLAc1_1e7_directIP_titration_rep1_Slot2-3_1_3512.12439.1538.2 2 2 1 8.1 2.4 4.21 5.69 68.2 5 0 0 11500.0 Hydrogen 17 (G) 9 QAAEIHATW (-) 9 GFQNIVDAY Q A/A/E/I H A\T\W 44.4 4 2 3 0 0 unbiased 42.973965 0.789447 513.7493 1026.5 -0.0096 -9.3 -1.6000000000000005 5.24 2779.1 nuORFdb_human ENST00000424300.5_1_3:49129835-49130708:- ENST00000424300.5_1_3:49129835-49130708:- QRICH1 5' uORF 5' uORF GN=QRICH1 5' uORF E_20221201_NO30_400nL_HLAc1_1e7_directIP_titration_rep1_Slot2-3_1_3512 10mio-rep2 E_20221201_NO30_400nL_HLAc1_1e7_directIP_titration_rep2_Slot2-3_1_3513.12395.1497.2 2 2 1 7.69 4.93 7.69 62.9 5 0 0 9060.0 Hydrogen 17 (G) 9 QAAEIHATW (-) 9 Q A/A/E/I H A|T\W 44.4 4 2 4 1 0 y-ion bias 42.82235 0.815365 513.7503 1026.5 -0.0076 -7.4 -0.7000000000000002 5.24 2779.1 nuORFdb_human ENST00000424300.5_1_3:49129835-49130708:- ENST00000424300.5_1_3:49129835-49130708:- QRICH1 5' uORF 5' uORF GN=QRICH1 5' uORF E_20221201_NO30_400nL_HLAc1_1e7_directIP_titration_rep2_Slot2-3_1_3513 10mio-rep3 E_20221201_NO30_400nL_HLAc1_1e7_directIP_titration_rep3_Slot2-3_1_3514.12373.1524.2 2 2 1 7.5 5.66 7.5 67.0 5 0 0 13100.0 Hydrogen 17 (G) 9 QAAEIHATW (-) 9 Q A/A/E/I H A\T\W 44.4 4 2 3 0 0 unbiased 42.749512 0.798628 513.7524 1026.5 -0.0036 -3.5 2.8 5.24 2779.1 nuORFdb_human ENST00000424300.5_1_3:49129835-49130708:- ENST00000424300.5_1_3:49129835-49130708:- QRICH1 5' uORF 5' uORF GN=QRICH1 5' uORF E_20221201_NO30_400nL_HLAc1_1e7_directIP_titration_rep3_Slot2-3_1_3514 40mio-rep1 E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep1_Slot1-04_1_3496.12621.1463.2 2 1 1 8.44 8.82 8.44 62.7 5 0 0 22000.0 Hydrogen 17 (G) 9 QAAEIHATW (-) 9 Q A/A/E/I H A|T\W 44.4 4 2 4 1 0 y-ion bias 43.597527 0.836918 513.7518 1026.5 -0.0036 -3.5 -0.2999999999999998 5.24 2779.1 nuORFdb_human ENST00000424300.5_1_3:49129835-49130708:- ENST00000424300.5_1_3:49129835-49130708:- QRICH1 5' uORF 5' uORF GN=QRICH1 5' uORF E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep1_Slot1-04_1_3496 40mio-rep2 E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep2_Slot2-5_1_3520.12322.1481.2 2 1 1 8.16 8.16 8.16 58.3 7 0 0 74300.0 Hydrogen 17 (G) 9 QAAEIHATW (-) 9 Q/A/A/E|I H-A/T\W 77.8 7 2 5 1 1 y-ion bias 42.567272 0.825588 513.7513 1026.5 -0.0056 -5.5 -0.5999999999999996 5.24 2779.1 nuORFdb_human ENST00000424300.5_1_3:49129835-49130708:- ENST00000424300.5_1_3:49129835-49130708:- QRICH1 5' uORF 5' uORF GN=QRICH1 5' uORF E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep2_Slot2-5_1_3520 40mio-rep4 E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep4_Slot2-5_1_3522.12333.1487.2 2 1 1 10.36 8.11 10.36 66.0 7 0 0 70300.0 Hydrogen 17 (G) 9 QAAEIHATW (-) 9 Q/A/A/E/I H-A|T\W 77.8 7 2 5 1 1 y-ion bias 42.590626 0.821949 513.751 1026.5 -0.0056 -5.5 -0.7999999999999998 5.24 2779.1 nuORFdb_human ENST00000424300.5_1_3:49129835-49130708:- ENST00000424300.5_1_3:49129835-49130708:- QRICH1 5' uORF 5' uORF GN=QRICH1 5' uORF E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep4_Slot2-5_1_3522 5mio-rep1 E_20221201_NO30_400nL_HLAc1_5e6_directIP_titration_rep1_Slot1-37_1_3502.12678.1502.2 2 1 1 8.19 2.63 4.32 5.56 67.3 6 0 0 3650.0 Hydrogen 17 (G) 9 QAAEIHATW (-) 9 AQGAGPTAAPW Q/A/A/E/I H-A T\W 55.6 5 1 4 0 1 y-ion bias 43.794407 0.812254 513.7486 1026.5 -0.0096 -9.3 -2.3000000000000007 5.24 2779.1 nuORFdb_human ENST00000424300.5_1_3:49129835-49130708:- ENST00000424300.5_1_3:49129835-49130708:- QRICH1 5' uORF 5' uORF GN=QRICH1 5' uORF E_20221201_NO30_400nL_HLAc1_5e6_directIP_titration_rep1_Slot1-37_1_3502 10mio-rep3 E_20221201_NO30_400nL_HLAc1_1e7_directIP_titration_rep3_Slot2-3_1_3514.8180.1566.2 2 2 1 8.8 4.9 4.94 3.9 80.4 6 1 1 5460.0 Hydrogen 71 (A) 8 QAAEPRVL (V) 8 QSSPRPVI Q/A/A/E|P R\V-L 75.0 6 2 4 1 1 y-ion bias 28.265886 0.77185 442.2511 883.5 -0.0049 -5.5 0.7999999999999998 6.0 10713.0 nuORFdb_human ENST00000374011.6_1_1:27878619-27879774:- ENST00000374011.6_1_1:27878619-27879774:- AHDC1 5' Overlap uORF 5' Overlap uORF GN=AHDC1 5' Overlap uORF E_20221201_NO30_400nL_HLAc1_1e7_directIP_titration_rep3_Slot2-3_1_3514 40mio-rep1 E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep1_Slot1-04_1_3496.8417.1453.2 2 2 1 10.4 5.8 5.02 4.59 85.7 6 1 1 28800.0 Hydrogen 71 (A) 8 QAAEPRVL (V) 8 QAAEPRVL|GAAAERPVL AQEARVPL Q/A/A/E/P R-V\L 75.0 6 1 4 0 1 y-ion bias 29.080746 0.842814 442.2512 883.5 -0.0049 -5.5 -2.3 6.0 10713.0 nuORFdb_human ENST00000374011.6_1_1:27878619-27879774:- ENST00000374011.6_1_1:27878619-27879774:-|ENST00000356534.7_1_3:184099099-184099356:+ AHDC1 5' Overlap uORF 5' Overlap uORF GN=AHDC1 5' Overlap uORF E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep1_Slot1-04_1_3496 40mio-rep2 E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep2_Slot2-5_1_3520.8124.1476.2 2 1 1 10.19 8.87 5.25 1.31 87.9 6 1 1 11400.0 Hydrogen 71 (A) 8 QAAEPRVL (V) 8 GAAAERPVL Q/A/A/E|P/R V\L 75.0 6 2 5 1 0 y-ion bias 28.068335 0.828493 442.2509 883.5 -0.0049 -5.5 -0.5999999999999996 6.0 10713.0 nuORFdb_human ENST00000374011.6_1_1:27878619-27879774:- ENST00000374011.6_1_1:27878619-27879774:- AHDC1 5' Overlap uORF 5' Overlap uORF GN=AHDC1 5' Overlap uORF E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep2_Slot2-5_1_3520 40mio-rep3 E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep3_Slot2-5_1_3521.8136.1487.2 2 1 1 9.94 8.21 5.35 1.73 86.7 6 1 1 10100.0 Hydrogen 71 (A) 8 QAAEPRVL (V) 8 GAAAERPVL Q/A/A/E|P/R V\L 75.0 6 2 5 1 0 y-ion bias 28.106716 0.82164 442.2513 883.5 -0.0049 -5.5 -0.9000000000000004 6.0 10713.0 nuORFdb_human ENST00000374011.6_1_1:27878619-27879774:- ENST00000374011.6_1_1:27878619-27879774:- AHDC1 5' Overlap uORF 5' Overlap uORF GN=AHDC1 5' Overlap uORF E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep3_Slot2-5_1_3521 40mio-rep4 E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep4_Slot2-5_1_3522.8136.1463.2 2 1 1 9.78 7.04 4.51 2.74 89.4 5 1 1 5660.0 Hydrogen 71 (A) 8 QAAEPRVL (V) 8 AQEARVPL Q/A/A/E|P R V\L 62.5 5 2 4 1 0 y-ion bias 28.101885 0.837062 442.2505 883.5 -0.0049 -5.5 -0.7999999999999998 6.0 10713.0 nuORFdb_human ENST00000374011.6_1_1:27878619-27879774:- ENST00000374011.6_1_1:27878619-27879774:- AHDC1 5' Overlap uORF 5' Overlap uORF GN=AHDC1 5' Overlap uORF E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep4_Slot2-5_1_3522 20mio-rep2 E_20221201_NO30_400nL_HLAc1_2e7_directIP_titration_rep3_Slot2-4_1_3518.18459.1550.2 2 1 1 7.33 4.72 6.0 2.61 70.5 6 1 1 2830.0 Hydrogen 18 (D) 8 QAAIARLF (L) 8 AQALRAFL Q/A/A/I/A R/L\F 75.0 6 1 5 0 0 y-ion bias 63.758629 0.781658 445.2648 889.525 -0.0027 -3.0 3.3 9.75 5583.4 nuORFdb_human ENST00000256015.4_1_12:92539441-92539582:- ENST00000256015.4_1_12:92539441-92539582:- BTG1 5' uORF 5' uORF GN=BTG1 5' uORF E_20221201_NO30_400nL_HLAc1_2e7_directIP_titration_rep3_Slot2-4_1_3518 40mio-rep2 E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep2_Slot2-5_1_3520.18280.1531.2 2 1 1 7.81 5.7 6.77 2.11 64.0 5 1 1 6120.0 Hydrogen 18 (D) 8 QAAIARLF (L) 8 AQALRAFL Q/A/A|I|A R L\F 62.5 5 3 4 2 0 unbiased 63.149406 0.793659 445.2623 889.525 -0.0087 -9.8 -4.9 9.75 5583.4 nuORFdb_human ENST00000256015.4_1_12:92539441-92539582:- ENST00000256015.4_1_12:92539441-92539582:- BTG1 5' uORF 5' uORF GN=BTG1 5' uORF E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep2_Slot2-5_1_3520 40mio-rep4 E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep4_Slot2-5_1_3522.18298.1529.2 2 1 1 7.02 4.53 5.16 2.49 75.9 6 1 1 4510.0 Hydrogen 18 (D) 8 QAAIARLF (L) 8 AQALRAFL Q/A/A/I/A R/L\F 75.0 6 1 5 0 0 y-ion bias 63.192112 0.795028 445.265 889.525 -0.0027 -3.0 1.7000000000000002 9.75 5583.4 nuORFdb_human ENST00000256015.4_1_12:92539441-92539582:- ENST00000256015.4_1_12:92539441-92539582:- BTG1 5' uORF 5' uORF GN=BTG1 5' uORF E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep4_Slot2-5_1_3522 40mio-rep1 E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep1_Slot1-04_1_3496.20730.512.1 1 1 1 9.73 4.03 6.47 5.7 76.1 7 0 0 3640.0 Hydrogen 13 (C) 9 QANGVALSW (S) 9 AVQNGALLC Q A/N/G\V|A\L\S\W 77.8 7 5 3 1 0 unbiased 71.611992 1.418889 945.4738 945.479 -0.0049 -5.1 -1.8999999999999995 5.52 4750.1 nuORFdb_human ENST00000454769.2_1_1:151171245-151171386:+ ENST00000454769.2_1_1:151171245-151171386:+ PIP5K1A 5' uORF 5' uORF GN=PIP5K1A 5' uORF E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep1_Slot1-04_1_3496 SCP E_20221201_NO30_400nL_HLAc1_4e7_PDAC_Slot2-6_1_3524.6882.1372.2 2 1 1 11.63 1.68 12.43 9.95 79.6 7 0 0 10200.0 Hydrogen 48 (D) 10 QASQECGHVF (-) 10 AAGSQTQAEGGGE Q A/S/Q/E|C/G H\V\F 60.0 6 3 5 1 0 unbiased Carbamidomethylation C:Carbamidomethylation, 23.779448 0.893747 581.7498 1162.495 -0.0019 -1.6 0.7999999999999998 5.24 6556.2 nuORFdb_human ENST00000452185.5_1_7:44161644-44161815:- ENST00000452185.5_1_7:44161644-44161815:-|ENST00000406581.6_1_7:44161644-44161898:-|ENST00000433715.5_1_7:44161644-44163931:-|ENST00000610533.4_1_7:44161641-44163135:- POLD2 5' Overlap uORF 5' Overlap uORF GN=POLD2 5' Overlap uORF E_20221201_NO30_400nL_HLAc1_4e7_PDAC_Slot2-6_1_3524 20mio-rep3 E_20221201_NO30_400nL_HLAc1_2e7_directIP_titration_rep4_Slot2-4_1_3519.19696.1474.2 2 1 1 10.18 2.8 6.28 7.39 74.5 6 0 0 7740.0 Hydrogen 119 (Q) 9 QATTVLHIL (K) 9 PGETRPLIL Q A/T/T/V L/H\I\L 55.6 5 2 4 0 0 y-ion bias 68.025665 0.82963 498.2947 995.588 -0.0057 -5.7 0.09999999999999964 6.74 18464.6 nuORFdb_human ENST00000399959.6_1_X:41200742-41202586:+ ENST00000399959.6_1_X:41200742-41202586:+ DDX3X Out-of-Frame Out-of-Frame GN=DDX3X Out-of-Frame E_20221201_NO30_400nL_HLAc1_2e7_directIP_titration_rep4_Slot2-4_1_3519 40mio-rep1 E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep1_Slot1-04_1_3496.19998.1447.2 2 4 1 10.56 2.14 6.51 8.41 71.3 8 0 0 28600.0 Hydrogen 119 (Q) 9 QATTVLHIL (K) 9 LSSVNLHLL Q/A/T/T/V\L/H\I\L 100.0 9 3 5 0 0 unbiased 69.083694 0.846751 498.295 995.588 -0.0057 -5.7 -2.5 6.74 18464.6 nuORFdb_human ENST00000399959.6_1_X:41200742-41202586:+ ENST00000399959.6_1_X:41200742-41202586:+ DDX3X Out-of-Frame Out-of-Frame GN=DDX3X Out-of-Frame E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep1_Slot1-04_1_3496 40mio-rep2 E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep2_Slot2-5_1_3520.19795.1465.2 2 4 1 11.44 3.05 7.6 8.39 75.3 7 0 0 25700.0 Hydrogen 119 (Q) 9 QATTVLHIL (K) 9 PGETRPLIL Q/A/T/T/V L/H\I\L 77.8 7 2 5 0 0 y-ion bias 68.382362 0.835428 498.295 995.588 -0.0057 -5.7 -0.7999999999999998 6.74 18464.6 nuORFdb_human ENST00000399959.6_1_X:41200742-41202586:+ ENST00000399959.6_1_X:41200742-41202586:+ DDX3X Out-of-Frame Out-of-Frame GN=DDX3X Out-of-Frame E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep2_Slot2-5_1_3520 40mio-rep3 E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep3_Slot2-5_1_3521.19757.1474.2 2 2 1 10.92 1.09 8.32 9.84 76.0 7 0 0 12700.0 Hydrogen 119 (Q) 9 QATTVLHIL (K) 9 PGETRPLIL Q/A/T/T/V L/H\I\L 77.8 7 2 5 0 0 y-ion bias 68.250381 0.829644 498.2955 995.588 -0.0057 -5.7 -1.1000000000000005 6.74 18464.6 nuORFdb_human ENST00000399959.6_1_X:41200742-41202586:+ ENST00000399959.6_1_X:41200742-41202586:+ DDX3X Out-of-Frame Out-of-Frame GN=DDX3X Out-of-Frame E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep3_Slot2-5_1_3521 40mio-rep4 E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep4_Slot2-5_1_3522.19744.1472.2 2 2 1 11.04 2.28 8.14 8.76 75.7 7 0 0 13100.0 Hydrogen 119 (Q) 9 QATTVLHIL (K) 9 PGETRPLIL Q/A/T/T/V L/H\I-L 77.8 7 1 5 0 1 y-ion bias 68.186195 0.830923 498.2958 995.588 -0.0037 -3.7 1.0 6.74 18464.6 nuORFdb_human ENST00000399959.6_1_X:41200742-41202586:+ ENST00000399959.6_1_X:41200742-41202586:+ DDX3X Out-of-Frame Out-of-Frame GN=DDX3X Out-of-Frame E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep4_Slot2-5_1_3522 Exploris_FAIMS VH20221207_MKP_FAIMS2022E_HLAc1_4e7_PDAC.49450.49595.2 2 1 1 11.84 5.9 7.58 5.94 78.2 7 0 0 13400000.0 Hydrogen 43 (G) 9 QAVEVLPHF (E) 9 IEVQGLPHF Q A\V|E/V-L/P/H/F 77.8 7 2 5 1 1 y-ion bias 0.96 87.4 87.85 47.46 -70.0 520.2811 1039.557 -0.0022 -2.2 0.3999999999999999 5.24 13478.5 nuORFdb_human ENST00000528150.1_1_1:78206598-78225465:- ENST00000528150.1_1_1:78206598-78225465:- USP33 5' Overlap uORF 5' Overlap uORF GN=USP33 5' Overlap uORF VH20221207_MKP_FAIMS2022E_HLAc1_4e7_PDAC 40mio-rep1 E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep1_Slot1-04_1_3496.17392.1288.2 2 2 1 7.58 3.73 7.58 58.2 6 0 1 10500.0 Hydrogen 14 (M) 10 QEDWPPKPVY (-) 10 Q E D/W|P/P K/P-V\Y 50.0 5 2 4 1 1 y-ion bias 60.076424 0.946066 629.8093 1258.61 0.0005 0.4 3.6 4.37 2679.1 nuORFdb_human ENST00000444129.6_1_12:21654122-21654191:- ENST00000444129.6_1_12:21654122-21654191:- RECQL 5' uORF 5' uORF GN=RECQL 5' uORF E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep1_Slot1-04_1_3496 20mio-rep1 E_20221201_NO30_400nL_HLAc1_2e7_directIP_titration_rep1_Slot2-4_1_3515.26511.1378.2 2 3 1 13.71 4.56 7.24 9.15 77.2 7 0 0 6940.0 Hydrogen 22 (K) 11 QEFPGGLGNVW (L) 11 DLGSEARGnAAAA Q E F|P G\G\L\G\N\V\W 54.5 6 7 1 1 0 b-ion bias 91.54705 0.890442 602.2879 1203.579 -0.0106 -8.8 -2.6000000000000005 4.0 7671.8 smORF_v4|nuORFdb_human DZIP1_13:96295855-96295511:- DZIP1_13:96295855-96295511:-|ENST00000361396.6_1_13:96295342-96295883:- DZIP1 uORF 13:96295855-96295511:- 13 DZIP1 5' uORF 5' uORF GN=DZIP1 E_20221201_NO30_400nL_HLAc1_2e7_directIP_titration_rep1_Slot2-4_1_3515 20mio-rep2 E_20221201_NO30_400nL_HLAc1_2e7_directIP_titration_rep3_Slot2-4_1_3518.26522.1376.2 2 4 1 11.88 5.85 5.26 6.04 75.3 6 0 0 7820.0 Hydrogen 22 (K) 11 QEFPGGLGNVW (L) 11 PnRPDTAFVW Q E F|P G G\L|G\N|V\W 45.5 5 6 3 3 0 b-ion bias 91.604256 0.891376 602.2893 1203.579 -0.0086 -7.1 -0.7999999999999998 4.0 7671.8 smORF_v4|nuORFdb_human DZIP1_13:96295855-96295511:- DZIP1_13:96295855-96295511:-|ENST00000361396.6_1_13:96295342-96295883:- DZIP1 uORF 13:96295855-96295511:- 13 DZIP1 5' uORF 5' uORF GN=DZIP1 E_20221201_NO30_400nL_HLAc1_2e7_directIP_titration_rep3_Slot2-4_1_3518 20mio-rep3 E_20221201_NO30_400nL_HLAc1_2e7_directIP_titration_rep4_Slot2-4_1_3519.26575.1367.2 2 2 1 11.38 2.31 6.96 9.07 76.6 6 0 0 4330.0 Hydrogen 22 (K) 11 QEFPGGLGNVW (L) 11 FGVFSnTQRF Q E F|P G G\L|G\N|V\W 45.5 5 6 3 3 0 b-ion bias 91.783714 0.89711 602.2901 1203.579 -0.0066 -5.5 0.2999999999999998 4.0 7671.8 smORF_v4|nuORFdb_human DZIP1_13:96295855-96295511:- DZIP1_13:96295855-96295511:-|ENST00000361396.6_1_13:96295342-96295883:- DZIP1 uORF 13:96295855-96295511:- 13 DZIP1 5' uORF 5' uORF GN=DZIP1 E_20221201_NO30_400nL_HLAc1_2e7_directIP_titration_rep4_Slot2-4_1_3519 40mio-rep1 E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep1_Slot1-04_1_3496.26729.1338.2 2 3 1 13.5 3.88 8.85 9.62 78.8 6 0 0 20000.0 Hydrogen 22 (K) 11 QEFPGGLGNVW (L) 11 EKHYnLNVW Q E F|P G G\L|G\N|V\W 45.5 5 6 3 3 0 b-ion bias 92.339073 0.914928 602.2888 1203.579 -0.0086 -7.1 -3.8999999999999995 4.0 7671.8 smORF_v4|nuORFdb_human DZIP1_13:96295855-96295511:- DZIP1_13:96295855-96295511:-|ENST00000361396.6_1_13:96295342-96295883:- DZIP1 uORF 13:96295855-96295511:- 13 DZIP1 5' uORF 5' uORF GN=DZIP1 E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep1_Slot1-04_1_3496 40mio-rep2 E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep2_Slot2-5_1_3520.26509.1360.2 2 2 1 13.78 4.47 8.56 9.3 83.5 7 0 0 11300.0 Hydrogen 22 (K) 11 QEFPGGLGNVW (L) 11 GDQVDTAGRVW Q E F|P G\G|L\G\N\V\W 54.5 6 7 2 2 0 b-ion bias 91.577164 0.901251 602.2887 1203.579 -0.0086 -7.1 -2.1999999999999993 4.0 7671.8 smORF_v4|nuORFdb_human DZIP1_13:96295855-96295511:- DZIP1_13:96295855-96295511:-|ENST00000361396.6_1_13:96295342-96295883:- DZIP1 uORF 13:96295855-96295511:- 13 DZIP1 5' uORF 5' uORF GN=DZIP1 E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep2_Slot2-5_1_3520 40mio-rep3 E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep3_Slot2-5_1_3521.26519.1360.2 2 2 1 12.69 4.03 7.29 8.66 80.7 6 0 0 8600.0 Hydrogen 22 (K) 11 QEFPGGLGNVW (L) 11 RDDEGnVIVW Q E F|P G G\L|G\N|V\W 45.5 5 6 3 3 0 b-ion bias 91.604736 0.901652 602.2918 1203.579 -0.0026 -2.1 2.4999999999999996 4.0 7671.8 smORF_v4|nuORFdb_human DZIP1_13:96295855-96295511:- DZIP1_13:96295855-96295511:-|ENST00000361396.6_1_13:96295342-96295883:- DZIP1 uORF 13:96295855-96295511:- 13 DZIP1 5' uORF 5' uORF GN=DZIP1 E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep3_Slot2-5_1_3521 40mio-rep4 E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep4_Slot2-5_1_3522.26613.1356.2 2 3 1 11.67 3.82 7.27 7.85 77.0 6 0 0 13500.0 Hydrogen 22 (K) 11 QEFPGGLGNVW (L) 11 AAAGASATQAQAW Q E F|P G G\L|G\N\V\W 45.5 5 6 2 2 0 b-ion bias 91.922401 0.903695 602.2902 1203.579 -0.0066 -5.5 -0.7999999999999998 4.0 7671.8 smORF_v4|nuORFdb_human DZIP1_13:96295855-96295511:- DZIP1_13:96295855-96295511:-|ENST00000361396.6_1_13:96295342-96295883:- DZIP1 uORF 13:96295855-96295511:- 13 DZIP1 5' uORF 5' uORF GN=DZIP1 E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep4_Slot2-5_1_3522 20mio-rep1 E_20221201_NO30_400nL_HLAc1_2e7_directIP_titration_rep1_Slot2-4_1_3515.29904.1391.2 2 1 1 10.39 2.86 10.39 7.52 72.1 7 0 0 4120.0 Q22q Hydrogen 22 (K) 11 qEFPGGLGNVW (L) 11 GENSGLPNQRD q E F|P/G/G\L|G N\V\W 54.5 6 5 4 2 0 unbiased q:Pyroglutamic acid 103.264282 0.881998 593.7748 1203.579 -17.0366 -8.5 -2.3 4.0 7671.8 smORF_v4|nuORFdb_human DZIP1_13:96295855-96295511:- DZIP1_13:96295855-96295511:-|ENST00000361396.6_1_13:96295342-96295883:- DZIP1 uORF 13:96295855-96295511:- 13 DZIP1 5' uORF 5' uORF GN=DZIP1 E_20221201_NO30_400nL_HLAc1_2e7_directIP_titration_rep1_Slot2-4_1_3515 20mio-rep2 E_20221201_NO30_400nL_HLAc1_2e7_directIP_titration_rep3_Slot2-4_1_3518.29915.1394.2 2 2 1 9.06 0.86 8.63 8.2 68.2 6 0 0 6800.0 Q22q Hydrogen 22 (K) 11 qEFPGGLGNVW (L) 11 GENSGLPNQRD q E F|P/G G\L\G N|V\W 36.4 4 5 3 2 0 unbiased q:Pyroglutamic acid 103.322006 0.88032 593.776 1203.579 -17.0346 -6.8 -0.5 4.0 7671.8 smORF_v4|nuORFdb_human DZIP1_13:96295855-96295511:- DZIP1_13:96295855-96295511:-|ENST00000361396.6_1_13:96295342-96295883:- DZIP1 uORF 13:96295855-96295511:- 13 DZIP1 5' uORF 5' uORF GN=DZIP1 E_20221201_NO30_400nL_HLAc1_2e7_directIP_titration_rep3_Slot2-4_1_3518 20mio-rep3 E_20221201_NO30_400nL_HLAc1_2e7_directIP_titration_rep4_Slot2-4_1_3519.29907.1387.2 2 1 1 10.09 3.85 7.08 6.24 73.9 8 0 0 2960.0 Q22q Hydrogen 22 (K) 11 qEFPGGLGNVW (L) 11 GENSGLPNQRD q E F|P/G\G\L\G\N|V\W 72.7 8 7 3 2 0 b-ion bias q:Pyroglutamic acid 103.294746 0.884398 593.775 1203.579 -17.0366 -8.5 -2.7 4.0 7671.8 smORF_v4|nuORFdb_human DZIP1_13:96295855-96295511:- DZIP1_13:96295855-96295511:-|ENST00000361396.6_1_13:96295342-96295883:- DZIP1 uORF 13:96295855-96295511:- 13 DZIP1 5' uORF 5' uORF GN=DZIP1 E_20221201_NO30_400nL_HLAc1_2e7_directIP_titration_rep4_Slot2-4_1_3519 40mio-rep1 E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep1_Slot1-04_1_3496.29931.195.1 1 3 1 17.81 3.6 12.27 14.21 98.3 9 0 0 12400.0 Q22q Hydrogen 22 (K) 11 qEFPGGLGNVW (L) 11 VAPNNSEQAADA q E/F/P\G|G|L|G\N\V\W 81.8 9 7 5 3 0 unbiased q:Pyroglutamic acid 103.401733 1.605883 1186.5483 1203.579 -17.0293 -2.3 0.9000000000000004 4.0 7671.8 smORF_v4|nuORFdb_human DZIP1_13:96295855-96295511:- DZIP1_13:96295855-96295511:-|ENST00000361396.6_1_13:96295342-96295883:- DZIP1 uORF 13:96295855-96295511:- 13 DZIP1 5' uORF 5' uORF GN=DZIP1 E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep1_Slot1-04_1_3496 40mio-rep2 E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep2_Slot2-5_1_3520.29904.211.1 1 5 1 16.6 3.44 14.62 13.17 97.0 8 0 0 17400.0 Q22q Hydrogen 22 (K) 11 qEFPGGLGNVW (L) 11 VAPNNSEQAADA q E/F/P G|G|L|G\N\V\W 63.6 7 6 5 3 0 unbiased q:Pyroglutamic acid 103.303459 1.596544 1186.5487 1203.579 -17.0293 -2.3 2.6000000000000005 4.0 7671.8 smORF_v4|nuORFdb_human DZIP1_13:96295855-96295511:- DZIP1_13:96295855-96295511:-|ENST00000361396.6_1_13:96295342-96295883:- DZIP1 uORF 13:96295855-96295511:- 13 DZIP1 5' uORF 5' uORF GN=DZIP1 E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep2_Slot2-5_1_3520 40mio-rep3 E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep3_Slot2-5_1_3521.29891.1395.2 2 6 1 15.15 4.48 11.2 10.67 86.6 8 0 0 20400.0 Q22q Hydrogen 22 (K) 11 qEFPGGLGNVW (L) 11 ETKHSEGVNW q E F|P|G\G\L|G\N\V\W 72.7 8 8 3 3 0 b-ion bias q:Pyroglutamic acid 103.25032 0.879446 593.7776 1203.579 -17.0306 -3.4 1.1999999999999997 4.0 7671.8 smORF_v4|nuORFdb_human DZIP1_13:96295855-96295511:- DZIP1_13:96295855-96295511:-|ENST00000361396.6_1_13:96295342-96295883:- DZIP1 uORF 13:96295855-96295511:- 13 DZIP1 5' uORF 5' uORF GN=DZIP1 E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep3_Slot2-5_1_3521 40mio-rep4 E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep4_Slot2-5_1_3522.29899.1394.2 2 4 1 12.91 2.7 10.53 10.22 81.6 8 0 0 13700.0 Q22q Hydrogen 22 (K) 11 qEFPGGLGNVW (L) 11 VAPNNSEQAADA q E F|P|G\G\L\G\N|V\W 72.7 8 8 3 3 0 b-ion bias q:Pyroglutamic acid 103.268188 0.879929 593.7752 1203.579 -17.0366 -8.5 -3.8 4.0 7671.8 smORF_v4|nuORFdb_human DZIP1_13:96295855-96295511:- DZIP1_13:96295855-96295511:-|ENST00000361396.6_1_13:96295342-96295883:- DZIP1 uORF 13:96295855-96295511:- 13 DZIP1 5' uORF 5' uORF GN=DZIP1 E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep4_Slot2-5_1_3522 40mio-rep1 E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep1_Slot1-04_1_3496.26201.334.1 1 1 1 10.41 5.92 3.12 4.5 77.7 5 0 0 3750.0 Hydrogen 5 (V) 9 QEIAVLQPF (W) 9 QLAIEQVFP Q E I\A|V\L|Q|P F 44.4 4 5 3 3 0 dual b/y bias 90.515503 1.524531 1044.5651 1044.572 -0.0024 -2.3 0.9000000000000004 4.0 3770.2 nuORFdb_human ENST00000454222.5_1_7:91456773-91509986:- ENST00000454222.5_1_7:91456773-91509986:-|ENST00000351870.7_1_7:91504015-91509986:-|ENST00000456229.1_1_7:91506204-91509986:-|ENST00000406735.6_1_7:91506280-91509986:- MTERF1 5' Overlap uORF 5' Overlap uORF GN=MTERF1 5' Overlap uORF E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep1_Slot1-04_1_3496 40mio-rep3 E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep3_Slot2-5_1_3521.25978.1451.2 2 1 1 7.02 4.12 2.62 2.91 67.5 6 0 0 3130.0 Hydrogen 5 (V) 9 QEIAVLQPF (W) 9 VSISPGQLPF Q E I\A\V\L\Q|P\F 66.7 6 6 1 1 0 b-ion bias 89.736778 0.844135 522.7878 1044.572 -0.0037 -3.5 1.0999999999999996 4.0 3770.2 nuORFdb_human ENST00000454222.5_1_7:91456773-91509986:- ENST00000454222.5_1_7:91456773-91509986:-|ENST00000351870.7_1_7:91504015-91509986:-|ENST00000456229.1_1_7:91506204-91509986:-|ENST00000406735.6_1_7:91506280-91509986:- MTERF1 5' Overlap uORF 5' Overlap uORF GN=MTERF1 5' Overlap uORF E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep3_Slot2-5_1_3521 40mio-rep1 E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep1_Slot1-04_1_3496.16237.1294.2 2 1 1 7.91 7.91 7.91 57.3 7 1 1 5320.0 Q52q Hydrogen 52 (S) 10 qELHSSRGLW (-) 10 q E/L/H/S\S R/G\L\W 60.0 6 3 4 0 0 unbiased q:Pyroglutamic acid 56.087063 0.942582 598.2948 1212.612 -17.0293 -2.3 0.9000000000000004 6.75 6313.0 nuORFdb_human ENST00000519234.5_3_8:62596669-62626935:- ENST00000519234.5_3_8:62596669-62626935:- ASPH Out-of-Frame Out-of-Frame GN=ASPH Out-of-Frame E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep1_Slot1-04_1_3496 40mio-rep4 E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep4_Slot2-5_1_3522.15896.1322.2 2 1 1 9.68 2.79 6.33 6.89 65.8 7 1 1 4160.0 Q52q Hydrogen 52 (S) 10 qELHSSRGLW (-) 10 QFNTQTALSW q E/L/H|S/S/R G\L\W 60.0 6 3 5 1 0 unbiased q:Pyroglutamic acid 54.897141 0.925262 598.2928 1212.612 -17.0333 -5.6 -0.8999999999999995 6.75 6313.0 nuORFdb_human ENST00000519234.5_3_8:62596669-62626935:- ENST00000519234.5_3_8:62596669-62626935:- ASPH Out-of-Frame Out-of-Frame GN=ASPH Out-of-Frame E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep4_Slot2-5_1_3522 10mio-rep1 E_20221201_NO30_400nL_HLAc1_1e7_directIP_titration_rep1_Slot2-3_1_3512.20351.1469.2 2 3 1 15.89 8.24 8.3 7.66 88.4 7 1 1 6180.0 Q53q Hydrogen 53 (F) 9 qEVQSLKGW (V) 9 qEVNTLRSQ q E/V|Q|S|L|K|G\W 77.8 7 6 6 5 0 dual b/y bias q:Pyroglutamic acid 70.299301 0.833283 529.2658 1074.558 -17.0331 -6.2 1.5 6.0 7990.3 nuORFdb_human ENST00000583334.5_3_17:30666835-30668280:- ENST00000583334.5_3_17:30666835-30668280:-|ENST00000577809.5_2_17:30666832-30669031:- C17orf75 3' Overlap dORF 3' Overlap dORF GN=C17orf75 3' Overlap dORF E_20221201_NO30_400nL_HLAc1_1e7_directIP_titration_rep1_Slot2-3_1_3512 10mio-rep2 E_20221201_NO30_400nL_HLAc1_1e7_directIP_titration_rep2_Slot2-3_1_3513.20280.1469.2 2 2 1 15.81 9.86 6.8 5.96 91.4 6 1 1 7020.0 Q53q Hydrogen 53 (F) 9 qEVQSLKGW (V) 9 qEVNTLRSQ q E/V|Q|S|L|K G\W 55.6 5 5 5 4 0 dual b/y bias q:Pyroglutamic acid 70.048759 0.832928 529.2658 1074.558 -17.0331 -6.2 0.5 6.0 7990.3 nuORFdb_human ENST00000583334.5_3_17:30666835-30668280:- ENST00000583334.5_3_17:30666835-30668280:-|ENST00000577809.5_2_17:30666832-30669031:- C17orf75 3' Overlap dORF 3' Overlap dORF GN=C17orf75 3' Overlap dORF E_20221201_NO30_400nL_HLAc1_1e7_directIP_titration_rep2_Slot2-3_1_3513 10mio-rep3 E_20221201_NO30_400nL_HLAc1_1e7_directIP_titration_rep3_Slot2-3_1_3514.20236.1468.2 2 1 1 12.84 6.9 5.94 5.94 77.3 7 1 1 3400.0 Q53q Hydrogen 53 (F) 9 qEVQSLKGW (V) 9 qDLQSRVTQ q E/V|Q|S|L|K/G\W 77.8 7 5 6 4 0 dual b/y bias q:Pyroglutamic acid 69.901474 0.833758 529.2669 1074.558 -17.0311 -4.3 2.0 6.0 7990.3 nuORFdb_human ENST00000583334.5_3_17:30666835-30668280:- ENST00000583334.5_3_17:30666835-30668280:-|ENST00000577809.5_2_17:30666832-30669031:- C17orf75 3' Overlap dORF 3' Overlap dORF GN=C17orf75 3' Overlap dORF E_20221201_NO30_400nL_HLAc1_1e7_directIP_titration_rep3_Slot2-3_1_3514 20mio-rep1 E_20221201_NO30_400nL_HLAc1_2e7_directIP_titration_rep1_Slot2-4_1_3515.20245.1464.2 2 2 1 14.7 8.63 6.87 6.07 87.3 7 1 1 10600.0 Q53q Hydrogen 53 (F) 9 qEVQSLKGW (V) 9 qEVNTLRSQ q E/V|Q|S|L|K/G\W 77.8 7 5 6 4 0 dual b/y bias q:Pyroglutamic acid 69.914551 0.836353 529.2665 1074.558 -17.0311 -4.3 1.9000000000000004 6.0 7990.3 nuORFdb_human ENST00000583334.5_3_17:30666835-30668280:- ENST00000583334.5_3_17:30666835-30668280:-|ENST00000577809.5_2_17:30666832-30669031:- C17orf75 3' Overlap dORF 3' Overlap dORF GN=C17orf75 3' Overlap dORF E_20221201_NO30_400nL_HLAc1_2e7_directIP_titration_rep1_Slot2-4_1_3515 20mio-rep2 E_20221201_NO30_400nL_HLAc1_2e7_directIP_titration_rep3_Slot2-4_1_3518.20293.1460.2 2 1 1 15.54 9.78 6.73 5.75 91.1 7 1 1 5480.0 Q53q Hydrogen 53 (F) 9 qEVQSLKGW (V) 9 qEVNTLRSQ q E|V|Q|S|L|K/G\W 77.8 7 6 6 5 0 dual b/y bias q:Pyroglutamic acid 70.096779 0.838743 529.2678 1074.558 -17.0291 -2.4 3.9 6.0 7990.3 nuORFdb_human ENST00000583334.5_3_17:30666835-30668280:- ENST00000583334.5_3_17:30666835-30668280:-|ENST00000577809.5_2_17:30666832-30669031:- C17orf75 3' Overlap dORF 3' Overlap dORF GN=C17orf75 3' Overlap dORF E_20221201_NO30_400nL_HLAc1_2e7_directIP_titration_rep3_Slot2-4_1_3518 20mio-rep3 E_20221201_NO30_400nL_HLAc1_2e7_directIP_titration_rep4_Slot2-4_1_3519.20266.1457.2 2 1 1 14.12 7.64 6.49 6.49 86.3 7 1 1 6180.0 Q53q Hydrogen 53 (F) 9 qEVQSLKGW (V) 9 qDLQSRVTQ q E/V|Q|S|L/K/G\W 77.8 7 4 6 3 0 dual b/y bias q:Pyroglutamic acid 69.993568 0.840398 529.267 1074.558 -17.0311 -4.3 1.5 6.0 7990.3 nuORFdb_human ENST00000583334.5_3_17:30666835-30668280:- ENST00000583334.5_3_17:30666835-30668280:-|ENST00000577809.5_2_17:30666832-30669031:- C17orf75 3' Overlap dORF 3' Overlap dORF GN=C17orf75 3' Overlap dORF E_20221201_NO30_400nL_HLAc1_2e7_directIP_titration_rep4_Slot2-4_1_3519 20mio-rep1 E_20221201_NO30_400nL_HLAc1_2e7_directIP_titration_rep1_Slot2-4_1_3515.6307.1634.3 3 3 1 9.77 4.38 7.78 5.39 73.4 6 2 2 24800.0 Q17q Hydrogen 17 (D) 10 qEVRQGLKAY (S) 10 qEVGRAPAVAPP q E/V|R\Q|G|L K A\Y 50.0 5 5 4 3 0 unbiased q:Pyroglutamic acid 21.791796 0.728556 392.2094 1191.648 -17.0356 -7.7 -1.5 8.59 18914.4 nuORFdb_human ENST00000405027.1_1_6:3978829-3979330:+ ENST00000405027.1_1_6:3978829-3979330:+ GLRX3P2 Pseudogene Pseudogene GN=GLRX3P2 Pseudogene E_20221201_NO30_400nL_HLAc1_2e7_directIP_titration_rep1_Slot2-4_1_3515 20mio-rep2 E_20221201_NO30_400nL_HLAc1_2e7_directIP_titration_rep3_Slot2-4_1_3518.6455.1665.3 3 2 1 9.3 3.5 6.96 5.8 75.2 6 2 2 11700.0 Q17q Hydrogen 17 (D) 10 qEVRQGLKAY (S) 10 qEVGRAPAVAPP q E/V|R|Q|G/L K\A Y 40.0 4 4 5 3 0 unbiased q:Pyroglutamic acid 22.304443 0.708983 392.2092 1191.648 -17.0356 -7.7 -1.4000000000000004 8.59 18914.4 nuORFdb_human ENST00000405027.1_1_6:3978829-3979330:+ ENST00000405027.1_1_6:3978829-3979330:+ GLRX3P2 Pseudogene Pseudogene GN=GLRX3P2 Pseudogene E_20221201_NO30_400nL_HLAc1_2e7_directIP_titration_rep3_Slot2-4_1_3518 20mio-rep3 E_20221201_NO30_400nL_HLAc1_2e7_directIP_titration_rep4_Slot2-4_1_3519.6342.1608.3 3 1 1 11.17 4.91 7.55 6.26 76.6 7 2 2 11500.0 Q17q Hydrogen 17 (D) 10 qEVRQGLKAY (S) 10 qEVGRAPAVAPP q E\V|R|Q|G/L K/A\Y 60.0 6 5 5 3 0 unbiased q:Pyroglutamic acid 21.911594 0.745049 392.2091 1191.648 -17.0356 -7.7 -1.9000000000000004 8.59 18914.4 nuORFdb_human ENST00000405027.1_1_6:3978829-3979330:+ ENST00000405027.1_1_6:3978829-3979330:+ GLRX3P2 Pseudogene Pseudogene GN=GLRX3P2 Pseudogene E_20221201_NO30_400nL_HLAc1_2e7_directIP_titration_rep4_Slot2-4_1_3519 40mio-rep2 E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep2_Slot2-5_1_3520.6307.1605.3 3 2 1 13.55 5.06 11.46 8.49 89.0 8 2 2 35600.0 Q17q Hydrogen 17 (D) 10 qEVRQGLKAY (S) 10 qEVGRAPAVAPP q E/V/R|Q|G|L-K-A\Y 80.0 8 4 5 3 2 unbiased q:Pyroglutamic acid 21.794939 0.747414 392.2097 1191.648 -17.0326 -5.2 -0.2999999999999998 8.59 18914.4 nuORFdb_human ENST00000405027.1_1_6:3978829-3979330:+ ENST00000405027.1_1_6:3978829-3979330:+ GLRX3P2 Pseudogene Pseudogene GN=GLRX3P2 Pseudogene E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep2_Slot2-5_1_3520 40mio-rep4 E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep4_Slot2-5_1_3522.6318.1606.3 3 1 1 8.64 4.48 5.92 4.16 77.5 6 2 2 18500.0 Q17q Hydrogen 17 (D) 10 qEVRQGLKAY (S) 10 qEVGRAPAVAPP q E/V/R|Q|G/L K-A Y 40.0 4 2 5 2 1 y-ion bias q:Pyroglutamic acid 21.82509 0.746804 392.2106 1191.648 -17.0296 -2.6 2.1 8.59 18914.4 nuORFdb_human ENST00000405027.1_1_6:3978829-3979330:+ ENST00000405027.1_1_6:3978829-3979330:+ GLRX3P2 Pseudogene Pseudogene GN=GLRX3P2 Pseudogene E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep4_Slot2-5_1_3522 10mio-rep2 E_20221201_NO30_400nL_HLAc1_1e7_directIP_titration_rep2_Slot2-3_1_3513.20480.573.1 1 1 1 11.66 5.38 5.98 6.28 76.0 7 0 0 2890.0 Hydrogen 7 (S) 9 QLFTGISAA (A) 9 FQTIGLSAA Q L/F|T\G\I\S\A\A 77.8 7 6 2 1 0 b-ion bias 70.739342 1.382402 907.4836 907.488 -0.0044 -4.8 1.9000000000000004 5.52 5911.7 smORF_v4|nuORFdb_human IFRD1_7:112090542-112090700:+ IFRD1_7:112090542-112090700:+|ENST00000403825.7_1_7:112090541-112090697:+ IFRD1 uORF 7:112090542-112090700:+ 7 IFRD1 5' uORF 5' uORF GN=IFRD1 E_20221201_NO30_400nL_HLAc1_1e7_directIP_titration_rep2_Slot2-3_1_3513 20mio-rep1 E_20221201_NO30_400nL_HLAc1_2e7_directIP_titration_rep1_Slot2-4_1_3515.20432.572.1 1 1 1 9.08 4.41 4.64 4.66 69.6 7 0 0 5660.0 Hydrogen 7 (S) 9 QLFTGISAA (A) 9 FQTIGLSAA Q L/F/T\G\I\S\A\A 77.8 7 5 2 0 0 b-ion bias 70.55999 1.38316 907.4835 907.488 -0.0054 -5.9 0.2999999999999998 5.52 5911.7 smORF_v4|nuORFdb_human IFRD1_7:112090542-112090700:+ IFRD1_7:112090542-112090700:+|ENST00000403825.7_1_7:112090541-112090697:+ IFRD1 uORF 7:112090542-112090700:+ 7 IFRD1 5' uORF 5' uORF GN=IFRD1 E_20221201_NO30_400nL_HLAc1_2e7_directIP_titration_rep1_Slot2-4_1_3515 20mio-rep2 E_20221201_NO30_400nL_HLAc1_2e7_directIP_titration_rep3_Slot2-4_1_3518.20483.564.1 1 2 1 10.14 4.31 4.45 5.83 73.7 7 0 0 10300.0 Hydrogen 7 (S) 9 QLFTGISAA (A) 9 FQTIGLSAA Q L/F|T\G\I\S\A\A 77.8 7 6 2 1 0 b-ion bias 70.752533 1.388073 907.4823 907.488 -0.0064 -7.0 -0.7000000000000002 5.52 5911.7 smORF_v4|nuORFdb_human IFRD1_7:112090542-112090700:+ IFRD1_7:112090542-112090700:+|ENST00000403825.7_1_7:112090541-112090697:+ IFRD1 uORF 7:112090542-112090700:+ 7 IFRD1 5' uORF 5' uORF GN=IFRD1 E_20221201_NO30_400nL_HLAc1_2e7_directIP_titration_rep3_Slot2-4_1_3518 20mio-rep3 E_20221201_NO30_400nL_HLAc1_2e7_directIP_titration_rep4_Slot2-4_1_3519.20476.564.1 1 1 1 8.28 3.78 4.76 4.5 72.5 7 0 0 4920.0 Hydrogen 7 (S) 9 QLFTGISAA (A) 9 FQTIGLSAA Q L/F|T\G\I\S\A\A 77.8 7 6 2 1 0 b-ion bias 70.72139 1.387591 907.4813 907.488 -0.0074 -8.1 -2.3 5.52 5911.7 smORF_v4|nuORFdb_human IFRD1_7:112090542-112090700:+ IFRD1_7:112090542-112090700:+|ENST00000403825.7_1_7:112090541-112090697:+ IFRD1 uORF 7:112090542-112090700:+ 7 IFRD1 5' uORF 5' uORF GN=IFRD1 E_20221201_NO30_400nL_HLAc1_2e7_directIP_titration_rep4_Slot2-4_1_3519 40mio-rep1 E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep1_Slot1-04_1_3496.20651.540.1 1 1 1 9.09 3.63 4.59 5.46 69.2 7 0 0 11200.0 Hydrogen 7 (S) 9 QLFTGISAA (A) 9 FQTIGLSAA Q L/F|T\G\I\S\A\A 77.8 7 6 2 1 0 b-ion bias 71.339226 1.40239 907.4846 907.488 -0.0034 -3.7 -0.5 5.52 5911.7 smORF_v4|nuORFdb_human IFRD1_7:112090542-112090700:+ IFRD1_7:112090542-112090700:+|ENST00000403825.7_1_7:112090541-112090697:+ IFRD1 uORF 7:112090542-112090700:+ 7 IFRD1 5' uORF 5' uORF GN=IFRD1 E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep1_Slot1-04_1_3496 40mio-rep2 E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep2_Slot2-5_1_3520.20374.560.1 1 3 1 10.65 5.29 3.73 5.37 74.4 6 0 0 28300.0 Hydrogen 7 (S) 9 QLFTGISAA (A) 9 FQTIGLSAA Q L/F|T G\I\S\A\A 55.6 5 5 2 1 0 b-ion bias 70.382065 1.390165 907.4854 907.488 -0.0034 -3.7 1.2000000000000002 5.52 5911.7 smORF_v4|nuORFdb_human IFRD1_7:112090542-112090700:+ IFRD1_7:112090542-112090700:+|ENST00000403825.7_1_7:112090541-112090697:+ IFRD1 uORF 7:112090542-112090700:+ 7 IFRD1 5' uORF 5' uORF GN=IFRD1 E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep2_Slot2-5_1_3520 40mio-rep3 E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep3_Slot2-5_1_3521.20368.559.1 1 2 1 9.27 2.17 6.28 7.1 71.9 7 0 0 10100.0 Hydrogen 7 (S) 9 QLFTGISAA (A) 9 FQTIGLSAA Q L/F|T\G\I\S\A\A 77.8 7 6 2 1 0 b-ion bias 70.36042 1.391082 907.4864 907.488 -0.0024 -2.6 1.9999999999999996 5.52 5911.7 smORF_v4|nuORFdb_human IFRD1_7:112090542-112090700:+ IFRD1_7:112090542-112090700:+|ENST00000403825.7_1_7:112090541-112090697:+ IFRD1 uORF 7:112090542-112090700:+ 7 IFRD1 5' uORF 5' uORF GN=IFRD1 E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep3_Slot2-5_1_3521 40mio-rep4 E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep4_Slot2-5_1_3522.20364.555.1 1 2 1 7.01 4.01 4.13 3.0 70.0 6 0 0 13300.0 Hydrogen 7 (S) 9 QLFTGISAA (A) 9 FQTIGLSAA Q L F|T\G\I\S\A\A 66.7 6 6 1 1 0 b-ion bias 70.330482 1.39296 907.4835 907.488 -0.0044 -4.8 -0.09999999999999964 5.52 5911.7 smORF_v4|nuORFdb_human IFRD1_7:112090542-112090700:+ IFRD1_7:112090542-112090700:+|ENST00000403825.7_1_7:112090541-112090697:+ IFRD1 uORF 7:112090542-112090700:+ 7 IFRD1 5' uORF 5' uORF GN=IFRD1 E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep4_Slot2-5_1_3522 10mio-rep2 E_20221201_NO30_400nL_HLAc1_1e7_directIP_titration_rep2_Slot2-3_1_3513.28788.581.1 1 1 1 7.48 5.04 2.93 2.45 66.4 6 0 0 1250.0 Q7q Hydrogen 7 (S) 9 qLFTGISAA (A) 9 VSANTGIAGT q L F\T|G\I\S\A-A 66.7 6 5 1 1 1 b-ion bias q:Pyroglutamic acid 99.427864 1.377719 890.4548 907.488 -17.0334 -7.6 -0.8999999999999995 5.52 5911.7 smORF_v4|nuORFdb_human IFRD1_7:112090542-112090700:+ IFRD1_7:112090542-112090700:+|ENST00000403825.7_1_7:112090541-112090697:+ IFRD1 uORF 7:112090542-112090700:+ 7 IFRD1 5' uORF 5' uORF GN=IFRD1 E_20221201_NO30_400nL_HLAc1_1e7_directIP_titration_rep2_Slot2-3_1_3513 20mio-rep3 E_20221201_NO30_400nL_HLAc1_2e7_directIP_titration_rep4_Slot2-4_1_3519.28777.570.1 1 2 1 8.93 7.69 2.81 1.24 71.3 5 0 0 5100.0 Q7q Hydrogen 7 (S) 9 qLFTGISAA (A) 9 VSANTGIAGT q L F|T|G\I\S\A A 44.4 4 5 2 2 0 b-ion bias q:Pyroglutamic acid 99.389816 1.383986 890.4577 907.488 -17.0304 -4.3 1.5 5.52 5911.7 smORF_v4|nuORFdb_human IFRD1_7:112090542-112090700:+ IFRD1_7:112090542-112090700:+|ENST00000403825.7_1_7:112090541-112090697:+ IFRD1 uORF 7:112090542-112090700:+ 7 IFRD1 5' uORF 5' uORF GN=IFRD1 E_20221201_NO30_400nL_HLAc1_2e7_directIP_titration_rep4_Slot2-4_1_3519 40mio-rep2 E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep2_Slot2-5_1_3520.28753.560.1 1 1 1 9.13 7.41 2.71 1.72 80.0 5 0 0 5060.0 Q7q Hydrogen 7 (S) 9 qLFTGISAA (A) 9 KENGTVTAA q L F|T\G|I\S\A A 44.4 4 5 2 2 0 b-ion bias q:Pyroglutamic acid 99.328781 1.390425 890.4536 907.488 -17.0344 -8.8 -3.9000000000000004 5.52 5911.7 smORF_v4|nuORFdb_human IFRD1_7:112090542-112090700:+ IFRD1_7:112090542-112090700:+|ENST00000403825.7_1_7:112090541-112090697:+ IFRD1 uORF 7:112090542-112090700:+ 7 IFRD1 5' uORF 5' uORF GN=IFRD1 E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep2_Slot2-5_1_3520 SCP E_20221201_NO30_400nL_HLAc1_4e7_PDAC_Slot2-6_1_3524.30551.208.1 1 1 1 10.3 8.64 1.3 1.66 79.9 6 0 0 12300.0 Hydrogen 26 (E) 9 QLIDLMLLL (Y) 9 LLQIDPFLI Q L I\D\L\M\L\L\L 66.7 6 6 0 0 0 b-ion bias 105.551521 1.598345 1071.646 1071.648 0.0018 1.7 4.1 3.8 7252.4 nuORFdb_human ENST00000483636.5_1_3:151488357-151501690:+ ENST00000483636.5_1_3:151488357-151501690:+ AADACP1 Pseudogene Pseudogene GN=AADACP1 Pseudogene E_20221201_NO30_400nL_HLAc1_4e7_PDAC_Slot2-6_1_3524 SCP E_20221201_NO30_400nL_HLAc1_4e7_PDAC_Slot2-6_1_3524.31781.224.1 1 1 1 10.37 5.66 6.42 4.71 95.6 6 0 0 18900.0 Q26q Hydrogen 26 (E) 9 qLIDLMLLL (Y) 9 qInLLFPII q L I\D\L\M\L\L\L 66.7 6 6 0 0 0 b-ion bias q:Pyroglutamic acid 109.798859 1.588848 1054.6191 1071.648 -17.0282 -1.6 0.7999999999999998 3.8 7252.4 nuORFdb_human ENST00000483636.5_1_3:151488357-151501690:+ ENST00000483636.5_1_3:151488357-151501690:+ AADACP1 Pseudogene Pseudogene GN=AADACP1 Pseudogene E_20221201_NO30_400nL_HLAc1_4e7_PDAC_Slot2-6_1_3524 10mio-rep1 E_20221201_NO30_400nL_HLAc1_1e7_directIP_titration_rep1_Slot2-3_1_3512.14329.1441.2 2 1 1 7.82 3.11 4.15 4.71 74.1 6 0 0 7590.0 Hydrogen 40 (L) 9 QLYSGHLQV (R) 9 GALSDARQQV Q/L/Y/S/G H-L Q\V 55.6 5 1 4 0 1 y-ion bias 49.500782 0.850892 522.7718 1044.547 -0.0105 -10.1 -2.3999999999999995 6.74 33108.1 nuORFdb_human ENST00000560634.1_1_15:84855599-84858179:+ ENST00000560634.1_1_15:84855599-84858179:+|ENST00000558282.5_1_15:85734698-85737265:+|ENST00000333235.3_1_Y:27624438-27626982:+|ENST00000558801.1_1_15:84960100-84962592:- CSPG4P11 Pseudogene Pseudogene GN=CSPG4P11 Pseudogene E_20221201_NO30_400nL_HLAc1_1e7_directIP_titration_rep1_Slot2-3_1_3512 10mio-rep2 E_20221201_NO30_400nL_HLAc1_1e7_directIP_titration_rep2_Slot2-3_1_3513.14284.1439.2 2 1 1 13.36 5.44 7.11 7.92 87.1 6 0 0 7720.0 Hydrogen 40 (L) 9 QLYSGHLQV (R) 9 LQYSVQVAH Q L|Y|S/G H|L\Q\V 55.6 5 5 4 3 0 dual b/y bias 49.345211 0.852051 522.7748 1044.547 -0.0045 -4.4 2.3 6.74 33108.1 nuORFdb_human ENST00000560634.1_1_15:84855599-84858179:+ ENST00000560634.1_1_15:84855599-84858179:+|ENST00000558282.5_1_15:85734698-85737265:+|ENST00000333235.3_1_Y:27624438-27626982:+|ENST00000558801.1_1_15:84960100-84962592:- CSPG4P11 Pseudogene Pseudogene GN=CSPG4P11 Pseudogene E_20221201_NO30_400nL_HLAc1_1e7_directIP_titration_rep2_Slot2-3_1_3513 10mio-rep3 E_20221201_NO30_400nL_HLAc1_1e7_directIP_titration_rep3_Slot2-3_1_3514.14245.1442.2 2 1 1 10.16 5.4 4.77 4.77 76.2 6 0 0 5310.0 Hydrogen 40 (L) 9 QLYSGHLQV (R) 9 GALSDARQQV Q/L/Y/S/G H L|Q\V 66.7 6 2 5 1 0 y-ion bias 49.212624 0.849881 522.7731 1044.547 -0.0085 -8.2 -1.8999999999999995 6.74 33108.1 nuORFdb_human ENST00000560634.1_1_15:84855599-84858179:+ ENST00000560634.1_1_15:84855599-84858179:+|ENST00000558282.5_1_15:85734698-85737265:+|ENST00000333235.3_1_Y:27624438-27626982:+|ENST00000558801.1_1_15:84960100-84962592:- CSPG4P11 Pseudogene Pseudogene GN=CSPG4P11 Pseudogene E_20221201_NO30_400nL_HLAc1_1e7_directIP_titration_rep3_Slot2-3_1_3514 20mio-rep1 E_20221201_NO30_400nL_HLAc1_2e7_directIP_titration_rep1_Slot2-4_1_3515.14217.1437.2 2 1 1 11.78 3.19 7.95 8.59 85.1 6 0 0 9910.0 Hydrogen 40 (L) 9 QLYSGHLQV (R) 9 GALSDARQQV Q L/Y|S/G H|L\Q\V 55.6 5 4 4 2 0 unbiased 49.103397 0.852906 522.774 1044.547 -0.0065 -6.3 -0.09999999999999964 6.74 33108.1 nuORFdb_human ENST00000560634.1_1_15:84855599-84858179:+ ENST00000560634.1_1_15:84855599-84858179:+|ENST00000558282.5_1_15:85734698-85737265:+|ENST00000333235.3_1_Y:27624438-27626982:+|ENST00000558801.1_1_15:84960100-84962592:- CSPG4P11 Pseudogene Pseudogene GN=CSPG4P11 Pseudogene E_20221201_NO30_400nL_HLAc1_2e7_directIP_titration_rep1_Slot2-4_1_3515 20mio-rep3 E_20221201_NO30_400nL_HLAc1_2e7_directIP_titration_rep4_Slot2-4_1_3519.14249.1431.2 2 1 1 11.19 5.05 5.83 6.14 80.5 7 0 0 9310.0 Hydrogen 40 (L) 9 QLYSGHLQV (R) 9 GALSDARQQV Q/L/Y/S/G H|L-Q\V 77.8 7 2 5 1 1 y-ion bias 49.217369 0.857222 522.774 1044.547 -0.0065 -6.3 -0.5 6.74 33108.1 nuORFdb_human ENST00000560634.1_1_15:84855599-84858179:+ ENST00000560634.1_1_15:84855599-84858179:+|ENST00000558282.5_1_15:85734698-85737265:+|ENST00000333235.3_1_Y:27624438-27626982:+|ENST00000558801.1_1_15:84960100-84962592:- CSPG4P11 Pseudogene Pseudogene GN=CSPG4P11 Pseudogene E_20221201_NO30_400nL_HLAc1_2e7_directIP_titration_rep4_Slot2-4_1_3519 40mio-rep1 E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep1_Slot1-04_1_3496.14481.1405.2 2 1 1 14.67 5.06 9.43 9.61 91.5 7 0 0 20700.0 Hydrogen 40 (L) 9 QLYSGHLQV (R) 9 QLSHNLSFV Q/L/Y|S/G H|L\Q\V 77.8 7 4 5 2 0 unbiased 50.021984 0.873123 522.7766 1044.547 -0.0005 -0.5 2.7 6.74 33108.1 nuORFdb_human ENST00000560634.1_1_15:84855599-84858179:+ ENST00000560634.1_1_15:84855599-84858179:+|ENST00000558282.5_1_15:85734698-85737265:+|ENST00000333235.3_1_Y:27624438-27626982:+|ENST00000558801.1_1_15:84960100-84962592:- CSPG4P11 Pseudogene Pseudogene GN=CSPG4P11 Pseudogene E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep1_Slot1-04_1_3496 40mio-rep2 E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep2_Slot2-5_1_3520.14191.1423.2 2 2 1 14.18 5.4 8.78 8.78 86.3 7 0 0 27300.0 Hydrogen 40 (L) 9 QLYSGHLQV (R) 9 GALSDARQQV Q/L/Y|S/G H|L\Q\V 77.8 7 4 5 2 0 unbiased 49.021782 0.861859 522.7754 1044.547 -0.0045 -4.4 0.5 6.74 33108.1 nuORFdb_human ENST00000560634.1_1_15:84855599-84858179:+ ENST00000560634.1_1_15:84855599-84858179:+|ENST00000558282.5_1_15:85734698-85737265:+|ENST00000333235.3_1_Y:27624438-27626982:+|ENST00000558801.1_1_15:84960100-84962592:- CSPG4P11 Pseudogene Pseudogene GN=CSPG4P11 Pseudogene E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep2_Slot2-5_1_3520 40mio-rep4 E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep4_Slot2-5_1_3522.14192.1423.2 2 1 1 11.17 4.64 6.53 6.53 84.0 7 0 0 21200.0 Hydrogen 40 (L) 9 QLYSGHLQV (R) 9 GALSDARQQV Q/L/Y|S/G H\L\Q\V 77.8 7 4 4 1 0 unbiased 49.014454 0.862216 522.7754 1044.547 -0.0045 -4.4 0.2999999999999998 6.74 33108.1 nuORFdb_human ENST00000560634.1_1_15:84855599-84858179:+ ENST00000560634.1_1_15:84855599-84858179:+|ENST00000558282.5_1_15:85734698-85737265:+|ENST00000333235.3_1_Y:27624438-27626982:+|ENST00000558801.1_1_15:84960100-84962592:- CSPG4P11 Pseudogene Pseudogene GN=CSPG4P11 Pseudogene E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep4_Slot2-5_1_3522 Eva-50mio-3fr AL20210827_EV_HLA_PDAC_PANFRO413_2D_50e6_W632_classI_fxn2.25384.25482.2 2 1 1 7.09 7.09 7.09 59.2 6 1 1 49000000.0 Hydrogen 8 (S) 10 QPYNFGTVKY (-) 10 Q P/Y/N/F/G T V/K|Y 50.0 5 1 6 1 0 y-ion bias 0.998 91.4 47.4 56.34 0.0 608.8034 1216.6 -0.0001 -0.1 -0.4 8.5 2006.3 nuORFdb_human ENST00000370473.4_1_1:89530912-89530966:- ENST00000370473.4_1_1:89530912-89530966:- GBP1 5' uORF 5' uORF GN=GBP1 5' uORF AL20210827_EV_HLA_PDAC_PANFRO413_2D_50e6_W632_classI_fxn2 Exploris AL20221201_MKP_PDAC_01.33168.33285.2 2 1 1 8.04 8.04 8.04 67.7 6 1 1 541000000.0 Hydrogen 8 (S) 10 QPYNFGTVKY (-) 10 Q P/Y/N/F/G T V/K|Y 50.0 5 1 6 1 0 y-ion bias 0.998 100.0 52.67 81.3 0.0 608.8039 1216.6 0.0009 0.8 0.0 8.5 2006.3 nuORFdb_human ENST00000370473.4_1_1:89530912-89530966:- ENST00000370473.4_1_1:89530912-89530966:- GBP1 5' uORF 5' uORF GN=GBP1 5' uORF AL20221201_MKP_PDAC_01 SCP E_20221201_NO30_400nL_HLAc1_4e7_PDAC_Slot2-6_1_3524.15611.1325.2 2 8 1 11.54 11.11 11.54 75.6 9 1 1 582000.0 Hydrogen 8 (S) 10 QPYNFGTVKY (-) 10 Q/P/Y/N/F/G/T/V|K\Y 100.0 10 2 8 1 0 y-ion bias 53.932453 0.923286 608.8024 1216.6 -0.003 -2.4 0.0 8.5 2006.3 nuORFdb_human ENST00000370473.4_1_1:89530912-89530966:- ENST00000370473.4_1_1:89530912-89530966:- GBP1 5' uORF 5' uORF GN=GBP1 5' uORF E_20221201_NO30_400nL_HLAc1_4e7_PDAC_Slot2-6_1_3524 SCP E_20221201_NO30_400nL_HLAc1_4e7_PDAC_Slot2-6_1_3524.15611.1424.2 2 3 1 13.8 3.57 9.33 10.23 84.3 8 1 1 15000.0 Q8q Hydrogen 8 (S) 10 qPYNFGTVKY (-) 10 AGSVCASASVPPP q P/Y/N|F|G\T/V|K\Y 80.0 8 5 6 3 0 unbiased q:Pyroglutamic acid 53.932453 0.861547 600.288 1216.6 -17.031 -3.7 -1.3000000000000003 8.5 2006.3 nuORFdb_human ENST00000370473.4_1_1:89530912-89530966:- ENST00000370473.4_1_1:89530912-89530966:- GBP1 5' uORF 5' uORF GN=GBP1 5' uORF E_20221201_NO30_400nL_HLAc1_4e7_PDAC_Slot2-6_1_3524 40mio-rep1 E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep1_Slot1-04_1_3496.24333.320.1 1 1 1 11.83 7.16 8.94 4.67 76.6 6 0 0 3760.0 Hydrogen 13 (L) 9 QSFEVGVQW (H) 9 SFIQDGVQW Q S F/E|V|G|V|Q\W 66.7 6 5 5 4 0 dual b/y bias 84.058968 1.532836 1079.5057 1079.516 -0.0056 -5.2 -2.0 4.0 3277.7 nuORFdb_human T214199_20:16845145-16854063:+ T214199_20:16845145-16854063:+ lincRNA lncRNA GN=nan lincRNA E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep1_Slot1-04_1_3496 40mio-rep1 E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep1_Slot1-04_1_3496.17199.1408.2 2 2 1 7.46 2.28 3.02 5.18 60.3 6 0 1 23200.0 Hydrogen 8 (R) 9 QTELSQLLK (I) 9 QETIQSLKL Q/T/E/L/S/Q/L L K 66.7 6 0 6 0 0 y-ion bias 59.410099 0.871329 530.3048 1059.604 -0.0017 -1.6 1.6 6.0 3638.3 nuORFdb_human ENST00000380591.7_1_5:72801034-72801127:+ ENST00000380591.7_1_5:72801034-72801127:+ BTF3 3' dORF 3' dORF GN=BTF3 3' dORF E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep1_Slot1-04_1_3496 40mio-rep4 E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep4_Slot2-5_1_3522.16899.1419.2 2 1 1 7.35 2.1 2.87 5.26 62.0 6 0 1 10500.0 Hydrogen 8 (R) 9 QTELSQLLK (I) 9 QETIQSLKL Q T/E/L/S/Q/L L\K 55.6 5 1 5 0 0 y-ion bias 58.360256 0.864166 530.3029 1059.604 -0.0057 -5.4 -0.7000000000000002 6.0 3638.3 nuORFdb_human ENST00000380591.7_1_5:72801034-72801127:+ ENST00000380591.7_1_5:72801034-72801127:+ BTF3 3' dORF 3' dORF GN=BTF3 3' dORF E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep4_Slot2-5_1_3522 20mio-rep2 E_20221201_NO30_400nL_HLAc1_2e7_directIP_titration_rep3_Slot2-4_1_3518.29459.1372.2 2 1 1 12.52 5.49 6.36 7.03 87.2 8 0 0 1420.0 Q2q Hydrogen 2 (I) 11 qTSSLPVNGLW (R) 11 GFSPAPKLACH q T S\S|L|P\V|N-G\L\W 72.7 8 7 3 3 1 b-ion bias q:Pyroglutamic acid 101.747322 0.894156 592.7961 1201.621 -17.0364 -8.3 -2.000000000000001 5.52 5278.0 nuORFdb_human ENST00000313608.12_1_17:55918309-55918571:- ENST00000313608.12_1_17:55918309-55918571:- MRPS23 Out-of-Frame Out-of-Frame GN=MRPS23 Out-of-Frame E_20221201_NO30_400nL_HLAc1_2e7_directIP_titration_rep3_Slot2-4_1_3518 20mio-rep3 E_20221201_NO30_400nL_HLAc1_2e7_directIP_titration_rep4_Slot2-4_1_3519.29459.1370.2 2 1 1 12.63 5.32 8.03 7.31 85.2 6 0 0 1980.0 Q2q Hydrogen 2 (I) 11 qTSSLPVNGLW (R) 11 GFSPAPKLACH q T S S|L|P V|N\G\L\W 45.5 5 6 3 3 0 b-ion bias q:Pyroglutamic acid 101.747154 0.895094 592.7985 1201.621 -17.0324 -5.0 0.7999999999999998 5.52 5278.0 nuORFdb_human ENST00000313608.12_1_17:55918309-55918571:- ENST00000313608.12_1_17:55918309-55918571:- MRPS23 Out-of-Frame Out-of-Frame GN=MRPS23 Out-of-Frame E_20221201_NO30_400nL_HLAc1_2e7_directIP_titration_rep4_Slot2-4_1_3519 40mio-rep1 E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep1_Slot1-04_1_3496.29498.180.1 1 2 1 12.04 5.58 7.47 6.46 88.0 7 0 0 7880.0 Q2q Hydrogen 2 (I) 11 qTSSLPVNGLW (R) 11 qQKLQEEIDA q T S S|L|P\V|N-G\L\W 63.6 7 6 3 3 1 b-ion bias q:Pyroglutamic acid 101.905769 1.614514 1184.5888 1201.621 -17.0312 -3.9 -0.6999999999999997 5.52 5278.0 nuORFdb_human ENST00000313608.12_1_17:55918309-55918571:- ENST00000313608.12_1_17:55918309-55918571:- MRPS23 Out-of-Frame Out-of-Frame GN=MRPS23 Out-of-Frame E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep1_Slot1-04_1_3496 40mio-rep2 E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep2_Slot2-5_1_3520.29452.1365.2 2 1 1 7.87 4.21 4.73 3.66 71.5 6 0 0 5630.0 Q2q Hydrogen 2 (I) 11 qTSSLPVNGLW (R) 11 GFSPAPKLACH q T S S|L|P V\N-G\L\W 45.5 5 5 2 2 1 b-ion bias q:Pyroglutamic acid 101.742393 0.898345 592.7962 1201.621 -17.0364 -8.3 -3.4000000000000004 5.52 5278.0 nuORFdb_human ENST00000313608.12_1_17:55918309-55918571:- ENST00000313608.12_1_17:55918309-55918571:- MRPS23 Out-of-Frame Out-of-Frame GN=MRPS23 Out-of-Frame E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep2_Slot2-5_1_3520 40mio-rep3 E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep3_Slot2-5_1_3521.29448.1371.2 2 2 1 10.52 5.64 5.39 4.87 82.2 6 0 0 8680.0 Q2q Hydrogen 2 (I) 11 qTSSLPVNGLW (R) 11 SSPnLPLGRQD q T S S|L|P V|N-G\L\W 45.5 5 5 3 3 1 unbiased q:Pyroglutamic acid 101.72049 0.894501 592.7994 1201.621 -17.0304 -3.3 1.2999999999999998 5.52 5278.0 nuORFdb_human ENST00000313608.12_1_17:55918309-55918571:- ENST00000313608.12_1_17:55918309-55918571:- MRPS23 Out-of-Frame Out-of-Frame GN=MRPS23 Out-of-Frame E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep3_Slot2-5_1_3521 40mio-rep4 E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep4_Slot2-5_1_3522.29452.1364.2 2 1 1 11.43 6.27 6.72 5.16 84.4 6 0 0 5380.0 Q2q Hydrogen 2 (I) 11 qTSSLPVNGLW (R) 11 GFSPAPKLACH q T S S|L|P V\N/G\L\W 45.5 5 5 3 2 0 unbiased q:Pyroglutamic acid 101.724846 0.898628 592.798 1201.621 -17.0324 -5.0 -0.2999999999999998 5.52 5278.0 nuORFdb_human ENST00000313608.12_1_17:55918309-55918571:- ENST00000313608.12_1_17:55918309-55918571:- MRPS23 Out-of-Frame Out-of-Frame GN=MRPS23 Out-of-Frame E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep4_Slot2-5_1_3522 40mio-rep3 E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep3_Slot2-5_1_3521.9381.1284.2 2 1 1 7.57 -0.09 7.66 7.66 62.0 6 0 0 2860.0 M98m M100m Hydrogen 92 (A) 10 QTYDQHmEmW (-) 10 TGSAPcGQVEmW Q T/Y/D|Q/H m E/m\W 50.0 5 2 5 1 0 y-ion bias m:Oxidized methionine 32.410255 0.949007 700.7631 1368.535 31.984 -4.2 0.39999999999999947 4.35 11099.3 nuORFdb_human ENST00000329047.12_1_17:75315924-75316227:+ ENST00000329047.12_1_17:75315924-75316227:+ SEPT9 5' uORF 5' uORF GN=SEPT9 5' uORF E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep3_Slot2-5_1_3521 SCP E_20221201_NO30_400nL_HLAc1_4e7_PDAC_Slot2-6_1_3524.22451.443.1 1 2 1 7.3 5.41 1.97 1.88 64.9 6 0 0 12500.0 Hydrogen 21 (S) 9 QVAGITVIY (A) 9 AGIINTVLY Q V A\G\I|T\V\I\Y 66.7 6 6 1 1 0 b-ion bias 77.563789 1.45986 963.5507 963.551 0.0 0.0 2.4 5.52 4668.1 nuORFdb_human ENST00000457483.5_1_2:61760979-61761771:- ENST00000457483.5_1_2:61760979-61761771:- XPO1 5' Overlap uORF 5' Overlap uORF GN=XPO1 5' Overlap uORF E_20221201_NO30_400nL_HLAc1_4e7_PDAC_Slot2-6_1_3524 SCP E_20221201_NO30_400nL_HLAc1_4e7_PDAC_Slot2-6_1_3524.21919.231.1 1 4 1 11.41 11.41 11.41 80.6 7 0 0 42800.0 Hydrogen 4 (K) 9 QYFMGCSTF (L) 9 Q Y|F|M\G|C\S\T\F 77.8 7 7 3 3 0 dual b/y bias Carbamidomethylation C:Carbamidomethylation, 75.723404 1.585035 1140.4489 1140.449 0.0011 1.0 3.4 5.52 1970.3 nuORFdb_human ENST00000484527.5_1_7:105917471-105917522:- ENST00000484527.5_1_7:105917471-105917522:- NAMPT ncRNA Processed Transcript lncRNA GN=NAMPT ncRNA Processed Transcript E_20221201_NO30_400nL_HLAc1_4e7_PDAC_Slot2-6_1_3524 Exploris_FAIMS VH20221207_MKP_FAIMS2022E_HLAc1_4e7_PDAC.53756.53872.2 2 1 1 7.85 2.35 6.07 5.5 55.7 6 0 0 42200000.0 Hydrogen 4 (K) 9 QYFMGCSTF (L) 9 SFGFEmEMF Q Y\F\M-G C-S/T/F 55.6 5 2 2 0 2 unbiased Carbamidomethylation C:Carbamidomethylation, 0.996 100.0 94.3 53.73 -50.0 570.7266 1140.449 -0.003 -2.7 -0.10000000000000009 5.52 1970.3 nuORFdb_human ENST00000484527.5_1_7:105917471-105917522:- ENST00000484527.5_1_7:105917471-105917522:- NAMPT ncRNA Processed Transcript lncRNA GN=NAMPT ncRNA Processed Transcript VH20221207_MKP_FAIMS2022E_HLAc1_4e7_PDAC Eva-50mio-3fr AL20210827_EV_HLA_PDAC_PANFRO413_2D_50e6_W632_classI_fxn2.34089.34089.2 2 3 2 10.64 6.27 5.5 4.37 82.0 6 1 1 126000000.0 Hydrogen 151 (G) 10 RAADAILTEF (G) 10 ETVRnGGLIF R A A D\A\I\L\T\E|F 60.0 6 6 1 1 0 b-ion bias 0.946 93.2 62.57 100.0 0.0 553.7959 1106.584 0.0005 0.4 0.10000000000000003 4.37 61769.9 nuORFdb_human ENST00000370082.1_1_20:62201547-62204491:- ENST00000370082.1_1_20:62201547-62204491:-|ENST00000479540.5_1_20:62203456-62204512:- HELZ2 5' Overlap uORF 5' Overlap uORF GN=HELZ2 5' Overlap uORF AL20210827_EV_HLA_PDAC_PANFRO413_2D_50e6_W632_classI_fxn2 Exploris_FAIMS VH20221207_MKP_FAIMS2022E_HLAc1_4e7_PDAC.50473.50473.2 2 1 1 10.5 5.58 5.81 4.92 78.7 6 1 1 7440000.0 Hydrogen 151 (G) 10 RAADAILTEF (G) 10 ETVRnGGLIF R A A D\A\I\L\T\E|F 60.0 6 6 1 1 0 b-ion bias 0.884 97.1 89.15 16.21 -50.0 553.7925 1106.584 -0.0063 -5.7 -3.1 4.37 61769.9 nuORFdb_human ENST00000370082.1_1_20:62201547-62204491:- ENST00000370082.1_1_20:62201547-62204491:-|ENST00000479540.5_1_20:62203456-62204512:- HELZ2 5' Overlap uORF 5' Overlap uORF GN=HELZ2 5' Overlap uORF VH20221207_MKP_FAIMS2022E_HLAc1_4e7_PDAC 20mio-rep3 E_20221201_NO30_400nL_HLAc1_2e7_directIP_titration_rep4_Slot2-4_1_3519.5770.1721.3 3 1 1 7.45 2.95 7.45 4.5 61.7 6 2 2 3770.0 Hydrogen 55 (R) 9 RAAEGVRAW (R) 9 ARAEGARER R A A|E|G|V/R\A\W 66.7 6 5 4 3 0 dual b/y bias 19.937109 0.673188 339.1839 1015.543 -0.0057 -5.7 0.09999999999999964 9.6 12095.4 smORF_v4 HCG11_6:26522170-26522520:+ HCG11_6:26522170-26522520:+ HCG11 lncRNA 6:26522170-26522520:+ 6 lincRNA GN=HCG11 E_20221201_NO30_400nL_HLAc1_2e7_directIP_titration_rep4_Slot2-4_1_3519 Exploris_FAIMS VH20221207_MKP_FAIMS2022E_HLAc1_4e7_PDAC.27138.27138.2 2 1 1 10.76 8.77 2.9 1.99 68.5 7 2 2 9550000.0 Hydrogen 12 (R) 9 RAALRAVTL (T) 9 RALRAGLTL R|A/A L\R\A\V|T|L 77.8 7 6 4 3 0 dual b/y bias 1.0 88.6 54.48 30.38 -70.0 485.8101 970.616 -0.0027 -2.8 -0.19999999999999973 12.0 8165.1 nuORFdb_human T021682_1:154644219-154644435:+ T021682_1:154644219-154644435:+|T042737_10:64025759-64041479:+ lincRNA lncRNA GN=nan lincRNA VH20221207_MKP_FAIMS2022E_HLAc1_4e7_PDAC 40mio-rep2 E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep2_Slot2-5_1_3520.3137.1533.2 2 2 1 8.94 6.64 2.3 2.3 66.8 5 2 2 90900.0 Hydrogen 2 (M) 8 RAKVSGSW (-) 8 ATLQKGSW R A K|V\S\G|S\W 62.5 5 5 2 2 0 b-ion bias 10.848483 0.792398 445.7424 890.484 -0.0076 -8.5 -3.5999999999999996 11.0 1021.2 nuORFdb_human ENST00000546485.5_1_12:56510571-56510598:+ ENST00000546485.5_1_12:56510571-56510598:+ RPL41 ncRNA Processed Transcript lncRNA GN=RPL41 ncRNA Processed Transcript E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep2_Slot2-5_1_3520 40mio-rep3 E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep3_Slot2-5_1_3521.3158.1530.2 2 3 1 12.91 6.98 4.67 5.93 79.9 6 2 2 57000.0 Hydrogen 2 (M) 8 RAKVSGSW (-) 8 GGRKISSW R A/K|V\S|G|S\W 75.0 6 5 4 3 0 dual b/y bias 10.917888 0.794886 445.7419 890.484 -0.0076 -8.5 -3.9000000000000004 11.0 1021.2 nuORFdb_human ENST00000546485.5_1_12:56510571-56510598:+ ENST00000546485.5_1_12:56510571-56510598:+ RPL41 ncRNA Processed Transcript lncRNA GN=RPL41 ncRNA Processed Transcript E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep3_Slot2-5_1_3521 40mio-rep4 E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep4_Slot2-5_1_3522.3170.1535.2 2 2 1 9.53 6.24 3.4 3.3 69.2 6 2 2 67800.0 Hydrogen 2 (M) 8 RAKVSGSW (-) 8 RLGKSGSEG R/A K/V\S\G/S\W 75.0 6 3 3 0 0 unbiased 10.954945 0.791409 445.743 890.484 -0.0056 -6.2 -1.5 11.0 1021.2 nuORFdb_human ENST00000546485.5_1_12:56510571-56510598:+ ENST00000546485.5_1_12:56510571-56510598:+ RPL41 ncRNA Processed Transcript lncRNA GN=RPL41 ncRNA Processed Transcript E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep4_Slot2-5_1_3522 10mio-rep1 E_20221201_NO30_400nL_HLAc1_1e7_directIP_titration_rep1_Slot2-3_1_3512.18480.1328.2 2 1 1 13.12 8.93 7.87 4.18 80.0 7 1 1 4130.0 Hydrogen 87 (L) 11 RALDPSLQAQW (S) 11 GEIRPSLQQEQ R A L/D|P\S L|Q\A|Q\W 54.5 6 6 4 3 0 unbiased 63.837448 0.921204 642.8334 1284.669 -0.0108 -8.4 -0.7000000000000002 5.84 14896.9 nuORFdb_human ENST00000432807.1_3_9:136891234-136891624:+ ENST00000432807.1_3_9:136891234-136891624:+|ENST00000603928.2_2_9:136891156-136891624:+ LINC00094 5' uORF 5' uORF GN=LINC00094 5' uORF E_20221201_NO30_400nL_HLAc1_1e7_directIP_titration_rep1_Slot2-3_1_3512 10mio-rep2 E_20221201_NO30_400nL_HLAc1_1e7_directIP_titration_rep2_Slot2-3_1_3513.18412.1333.2 2 3 1 15.15 7.33 8.92 7.82 80.8 6 1 1 9660.0 Hydrogen 87 (L) 11 RALDPSLQAQW (S) 11 AGGGLGLAGQVAAGW R A L\D|P S L|Q|A|Q\W 45.5 5 6 4 4 0 dual b/y bias 63.599407 0.918517 642.8329 1284.669 -0.0108 -8.4 -1.7000000000000002 5.84 14896.9 nuORFdb_human ENST00000432807.1_3_9:136891234-136891624:+ ENST00000432807.1_3_9:136891234-136891624:+|ENST00000603928.2_2_9:136891156-136891624:+ LINC00094 5' uORF 5' uORF GN=LINC00094 5' uORF E_20221201_NO30_400nL_HLAc1_1e7_directIP_titration_rep2_Slot2-3_1_3513 10mio-rep3 E_20221201_NO30_400nL_HLAc1_1e7_directIP_titration_rep3_Slot2-3_1_3514.18366.1325.2 2 1 1 12.88 6.28 7.51 6.6 82.0 6 1 1 3620.0 Hydrogen 87 (L) 11 RALDPSLQAQW (S) 11 GEIRPSLQQEQ R A L\D|P S L|Q|A|Q\W 45.5 5 6 4 4 0 dual b/y bias 63.442486 0.923253 642.8355 1284.669 -0.0068 -5.3 1.0 5.84 14896.9 nuORFdb_human ENST00000432807.1_3_9:136891234-136891624:+ ENST00000432807.1_3_9:136891234-136891624:+|ENST00000603928.2_2_9:136891156-136891624:+ LINC00094 5' uORF 5' uORF GN=LINC00094 5' uORF E_20221201_NO30_400nL_HLAc1_1e7_directIP_titration_rep3_Slot2-3_1_3514 20mio-rep1 E_20221201_NO30_400nL_HLAc1_2e7_directIP_titration_rep1_Slot2-4_1_3515.18397.1315.2 2 1 1 16.21 7.57 10.77 8.63 87.6 7 1 1 5680.0 Hydrogen 87 (L) 11 RALDPSLQAQW (S) 11 AGGGLGLAGQVAAGW R A L\D|P\S L|Q|A|Q\W 54.5 6 7 4 4 0 dual b/y bias 63.535164 0.929479 642.8343 1284.669 -0.0088 -6.8 -0.5999999999999996 5.84 14896.9 nuORFdb_human ENST00000432807.1_3_9:136891234-136891624:+ ENST00000432807.1_3_9:136891234-136891624:+|ENST00000603928.2_2_9:136891156-136891624:+ LINC00094 5' uORF 5' uORF GN=LINC00094 5' uORF E_20221201_NO30_400nL_HLAc1_2e7_directIP_titration_rep1_Slot2-4_1_3515 20mio-rep2 E_20221201_NO30_400nL_HLAc1_2e7_directIP_titration_rep3_Slot2-4_1_3518.18463.1311.2 2 2 1 12.83 5.67 8.95 7.15 79.2 6 1 1 13800.0 Hydrogen 87 (L) 11 RALDPSLQAQW (S) 11 GEIRPSLQQEQ R A L D|P\S L|Q|A|Q\W 45.5 5 6 4 4 0 dual b/y bias 63.772438 0.932019 642.8344 1284.669 -0.0088 -6.8 -0.5 5.84 14896.9 nuORFdb_human ENST00000432807.1_3_9:136891234-136891624:+ ENST00000432807.1_3_9:136891234-136891624:+|ENST00000603928.2_2_9:136891156-136891624:+ LINC00094 5' uORF 5' uORF GN=LINC00094 5' uORF E_20221201_NO30_400nL_HLAc1_2e7_directIP_titration_rep3_Slot2-4_1_3518 20mio-rep3 E_20221201_NO30_400nL_HLAc1_2e7_directIP_titration_rep4_Slot2-4_1_3519.18443.1321.2 2 1 1 14.32 5.72 10.25 8.6 88.3 6 1 1 5540.0 Hydrogen 87 (L) 11 RALDPSLQAQW (S) 11 AGGGLGLAGQVAAGW R A L\D|P S L|Q|A|Q\W 45.5 5 6 4 4 0 dual b/y bias 63.699589 0.925605 642.8325 1284.669 -0.0108 -8.4 -2.6000000000000005 5.84 14896.9 nuORFdb_human ENST00000432807.1_3_9:136891234-136891624:+ ENST00000432807.1_3_9:136891234-136891624:+|ENST00000603928.2_2_9:136891156-136891624:+ LINC00094 5' uORF 5' uORF GN=LINC00094 5' uORF E_20221201_NO30_400nL_HLAc1_2e7_directIP_titration_rep4_Slot2-4_1_3519 40mio-rep1 E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep1_Slot1-04_1_3496.18683.1277.2 2 5 1 15.09 6.65 10.91 8.44 82.3 6 1 1 51200.0 Hydrogen 87 (L) 11 RALDPSLQAQW (S) 11 GEIRPSLQQEQ R A L\D|P S L|Q|A|Q\W 45.5 5 6 4 4 0 dual b/y bias 64.54084 0.953011 642.8349 1284.669 -0.0068 -5.3 -2.0999999999999996 5.84 14896.9 nuORFdb_human ENST00000432807.1_3_9:136891234-136891624:+ ENST00000432807.1_3_9:136891234-136891624:+|ENST00000603928.2_2_9:136891156-136891624:+ LINC00094 5' uORF 5' uORF GN=LINC00094 5' uORF E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep1_Slot1-04_1_3496 40mio-rep2 E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep2_Slot2-5_1_3520.18449.1307.2 2 3 1 13.81 6.54 8.35 7.27 82.7 6 1 1 30000.0 Hydrogen 87 (L) 11 RALDPSLQAQW (S) 11 AGGGLGLAGQVAAGW R A L\D|P S L|Q|A|Q\W 45.5 5 6 4 4 0 dual b/y bias 63.732838 0.93423 642.8344 1284.669 -0.0088 -6.8 -1.8999999999999995 5.84 14896.9 nuORFdb_human ENST00000432807.1_3_9:136891234-136891624:+ ENST00000432807.1_3_9:136891234-136891624:+|ENST00000603928.2_2_9:136891156-136891624:+ LINC00094 5' uORF 5' uORF GN=LINC00094 5' uORF E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep2_Slot2-5_1_3520 40mio-rep3 E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep3_Slot2-5_1_3521.18437.1307.2 2 4 1 14.9 6.26 9.12 8.63 82.3 7 1 1 30700.0 Hydrogen 87 (L) 11 RALDPSLQAQW (S) 11 GRPGGSRGRAAGW R A L/D|P\S L|Q|A|Q\W 54.5 6 6 5 4 0 dual b/y bias 63.68961 0.934458 642.8374 1284.669 -0.0028 -2.2 2.3999999999999995 5.84 14896.9 nuORFdb_human ENST00000432807.1_3_9:136891234-136891624:+ ENST00000432807.1_3_9:136891234-136891624:+|ENST00000603928.2_2_9:136891156-136891624:+ LINC00094 5' uORF 5' uORF GN=LINC00094 5' uORF E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep3_Slot2-5_1_3521 40mio-rep4 E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep4_Slot2-5_1_3522.18419.1304.2 2 3 1 14.81 7.64 7.69 7.17 86.5 6 1 1 26700.0 Hydrogen 87 (L) 11 RALDPSLQAQW (S) 11 GRPGGSRGRAAGW R A L\D|P S L|Q|A|Q\W 45.5 5 6 4 4 0 dual b/y bias 63.610863 0.936576 642.8352 1284.669 -0.0068 -5.3 -0.5999999999999996 5.84 14896.9 nuORFdb_human ENST00000432807.1_3_9:136891234-136891624:+ ENST00000432807.1_3_9:136891234-136891624:+|ENST00000603928.2_2_9:136891156-136891624:+ LINC00094 5' uORF 5' uORF GN=LINC00094 5' uORF E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep4_Slot2-5_1_3522 5mio-rep1 E_20221201_NO30_400nL_HLAc1_5e6_directIP_titration_rep1_Slot1-37_1_3502.18710.1323.2 2 1 1 7.42 6.02 3.08 1.4 66.3 6 1 1 4530.0 Hydrogen 87 (L) 11 RALDPSLQAQW (S) 11 AGGGLGLAGQVAAGW R A L D\P S-L\Q|A|Q\W 45.5 5 5 2 2 1 b-ion bias 64.624847 0.924676 642.8334 1284.669 -0.0108 -8.4 -1.4000000000000004 5.84 14896.9 nuORFdb_human ENST00000432807.1_3_9:136891234-136891624:+ ENST00000432807.1_3_9:136891234-136891624:+|ENST00000603928.2_2_9:136891156-136891624:+ LINC00094 5' uORF 5' uORF GN=LINC00094 5' uORF E_20221201_NO30_400nL_HLAc1_5e6_directIP_titration_rep1_Slot1-37_1_3502 40mio-rep2 E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep2_Slot2-5_1_3520.7990.1516.2 2 1 1 11.22 8.55 3.19 2.67 88.9 5 1 1 11000.0 M22m Hydrogen 20 (Q) 9 RAmSDPAAW (C) 9 WSQSANAAW R A m S/D|P\A|A\W 55.6 5 4 3 2 0 unbiased m:Oxidized methionine 27.605822 0.803611 510.7297 1004.462 15.9909 -3.9 1.0000000000000004 5.84 5706.5 nuORFdb_human ENST00000329198.4_1_5:172659283-172659433:- ENST00000329198.4_1_5:172659283-172659433:- NKX2-5 3' dORF 3' dORF GN=NKX2-5 3' dORF E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep2_Slot2-5_1_3520 1mio-rep1 E_20221201_NO30_400nL_HLAc1_1e6_directIP_titration_rep1_Slot1-06_1_3498.15395.1490.2 2 2 1 13.0 7.05 6.32 5.95 85.2 6 1 1 6550.0 Hydrogen 38 (V) 9 RAVNEFSGW (T) 9 NFSWVNLGE R A V|N|E|F|S|G\W 66.7 6 6 5 5 0 dual b/y bias 53.173237 0.819557 533.2569 1065.511 -0.0045 -4.2 0.7000000000000002 6.0 9072.2 nuORFdb_human ENST00000345662.5_1_2:32288779-32289037:+ ENST00000345662.5_1_2:32288779-32289037:+ SPAST 5' Overlap uORF 5' Overlap uORF GN=SPAST 5' Overlap uORF E_20221201_NO30_400nL_HLAc1_1e6_directIP_titration_rep1_Slot1-06_1_3498 10mio-rep1 E_20221201_NO30_400nL_HLAc1_1e7_directIP_titration_rep1_Slot2-3_1_3512.15115.1539.2 2 5 1 12.9 6.61 6.09 6.3 87.6 6 1 1 28100.0 Hydrogen 38 (V) 9 RAVNEFSGW (T) 9 NFSWVNLGE R A V|N|E|F|S|G\W 66.7 6 6 5 5 0 dual b/y bias 52.216454 0.78863 533.2552 1065.511 -0.0085 -8.0 -0.2999999999999998 6.0 9072.2 nuORFdb_human ENST00000345662.5_1_2:32288779-32289037:+ ENST00000345662.5_1_2:32288779-32289037:+ SPAST 5' Overlap uORF 5' Overlap uORF GN=SPAST 5' Overlap uORF E_20221201_NO30_400nL_HLAc1_1e7_directIP_titration_rep1_Slot2-3_1_3512 10mio-rep2 E_20221201_NO30_400nL_HLAc1_1e7_directIP_titration_rep2_Slot2-3_1_3513.15051.1494.2 2 5 1 11.98 5.69 5.89 6.28 82.1 6 1 1 27700.0 Hydrogen 38 (V) 9 RAVNEFSGW (T) 9 NFSWVNLGE R A V|N|E|F|S|G\W 66.7 6 6 5 5 0 dual b/y bias 51.993336 0.817321 533.2559 1065.511 -0.0065 -6.1 0.6000000000000005 6.0 9072.2 nuORFdb_human ENST00000345662.5_1_2:32288779-32289037:+ ENST00000345662.5_1_2:32288779-32289037:+ SPAST 5' Overlap uORF 5' Overlap uORF GN=SPAST 5' Overlap uORF E_20221201_NO30_400nL_HLAc1_1e7_directIP_titration_rep2_Slot2-3_1_3513 10mio-rep3 E_20221201_NO30_400nL_HLAc1_1e7_directIP_titration_rep3_Slot2-3_1_3514.15074.1499.2 2 3 1 12.81 8.03 5.66 4.78 85.3 6 1 1 23300.0 Hydrogen 38 (V) 9 RAVNEFSGW (T) 9 NFSWVNLGE R A V|N\E|F|S|G\W 66.7 6 6 4 4 0 dual b/y bias 52.075035 0.813968 533.2575 1065.511 -0.0025 -2.3 4.0 6.0 9072.2 nuORFdb_human ENST00000345662.5_1_2:32288779-32289037:+ ENST00000345662.5_1_2:32288779-32289037:+ SPAST 5' Overlap uORF 5' Overlap uORF GN=SPAST 5' Overlap uORF E_20221201_NO30_400nL_HLAc1_1e7_directIP_titration_rep3_Slot2-3_1_3514 20mio-rep1 E_20221201_NO30_400nL_HLAc1_2e7_directIP_titration_rep1_Slot2-4_1_3515.14963.1523.2 2 5 1 15.06 8.27 7.04 6.79 91.3 6 1 1 56500.0 Hydrogen 38 (V) 9 RAVNEFSGW (T) 9 KAQTKCSGW R A V|N\E|F|S|G\W 66.7 6 6 4 4 0 dual b/y bias 51.678528 0.798898 533.2563 1065.511 -0.0065 -6.1 0.10000000000000053 6.0 9072.2 nuORFdb_human ENST00000345662.5_1_2:32288779-32289037:+ ENST00000345662.5_1_2:32288779-32289037:+ SPAST 5' Overlap uORF 5' Overlap uORF GN=SPAST 5' Overlap uORF E_20221201_NO30_400nL_HLAc1_2e7_directIP_titration_rep1_Slot2-4_1_3515 20mio-rep2 E_20221201_NO30_400nL_HLAc1_2e7_directIP_titration_rep3_Slot2-4_1_3518.15108.1517.2 2 5 1 13.73 8.18 6.43 5.55 89.9 6 1 1 54100.0 Hydrogen 38 (V) 9 RAVNEFSGW (T) 9 DRGQLFSWG R A V\N|E|F|S|G\W 66.7 6 6 4 4 0 dual b/y bias 52.183483 0.802939 533.257 1065.511 -0.0045 -4.2 2.0999999999999996 6.0 9072.2 nuORFdb_human ENST00000345662.5_1_2:32288779-32289037:+ ENST00000345662.5_1_2:32288779-32289037:+ SPAST 5' Overlap uORF 5' Overlap uORF GN=SPAST 5' Overlap uORF E_20221201_NO30_400nL_HLAc1_2e7_directIP_titration_rep3_Slot2-4_1_3518 20mio-rep3 E_20221201_NO30_400nL_HLAc1_2e7_directIP_titration_rep4_Slot2-4_1_3519.14999.1491.2 2 5 1 16.75 8.77 7.98 7.98 95.4 6 1 1 45800.0 Hydrogen 38 (V) 9 RAVNEFSGW (T) 9 ISGVSCLSGW R A V|N|E|F|S|G|W 66.7 6 6 6 6 0 dual b/y bias 51.809517 0.819493 533.2555 1065.511 -0.0065 -6.1 -0.2999999999999998 6.0 9072.2 nuORFdb_human ENST00000345662.5_1_2:32288779-32289037:+ ENST00000345662.5_1_2:32288779-32289037:+ SPAST 5' Overlap uORF 5' Overlap uORF GN=SPAST 5' Overlap uORF E_20221201_NO30_400nL_HLAc1_2e7_directIP_titration_rep4_Slot2-4_1_3519 40mio-rep1 E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep1_Slot1-04_1_3496.15270.1463.2 2 3 1 19.34 11.49 7.92 7.84 96.8 6 1 1 158000.0 Hydrogen 38 (V) 9 RAVNEFSGW (T) 9 NFSWVNLGE R A V|N|E|F|S|G\W 66.7 6 6 5 5 0 dual b/y bias 52.746571 0.836889 533.2567 1065.511 -0.0045 -4.2 -1.0 6.0 9072.2 nuORFdb_human ENST00000345662.5_1_2:32288779-32289037:+ ENST00000345662.5_1_2:32288779-32289037:+ SPAST 5' Overlap uORF 5' Overlap uORF GN=SPAST 5' Overlap uORF E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep1_Slot1-04_1_3496 40mio-rep2 E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep2_Slot2-5_1_3520.14940.1480.2 2 5 1 19.37 11.36 8.0 8.0 97.5 6 1 1 169000.0 Hydrogen 38 (V) 9 RAVNEFSGW (T) 9 ISGVSCLSGW R A V|N|E|F|S|G\W 66.7 6 6 5 5 0 dual b/y bias 51.608418 0.826201 533.257 1065.511 -0.0045 -4.2 0.7000000000000002 6.0 9072.2 nuORFdb_human ENST00000345662.5_1_2:32288779-32289037:+ ENST00000345662.5_1_2:32288779-32289037:+ SPAST 5' Overlap uORF 5' Overlap uORF GN=SPAST 5' Overlap uORF E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep2_Slot2-5_1_3520 40mio-rep3 E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep3_Slot2-5_1_3521.14973.1481.2 2 6 1 18.32 11.38 7.46 6.94 96.6 6 1 1 134000.0 Hydrogen 38 (V) 9 RAVNEFSGW (T) 9 TRCSVLSGW R A V|N|E|F|S|G\W 66.7 6 6 5 5 0 dual b/y bias 51.7248 0.825395 533.2573 1065.511 -0.0045 -4.2 0.39999999999999947 6.0 9072.2 nuORFdb_human ENST00000345662.5_1_2:32288779-32289037:+ ENST00000345662.5_1_2:32288779-32289037:+ SPAST 5' Overlap uORF 5' Overlap uORF GN=SPAST 5' Overlap uORF E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep3_Slot2-5_1_3521 40mio-rep4 E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep4_Slot2-5_1_3522.14973.1483.2 2 5 1 19.36 10.85 8.51 8.51 97.3 6 1 1 162000.0 Hydrogen 38 (V) 9 RAVNEFSGW (T) 9 ISGVSCLSGW R A V|N|E|F|S|G\W 66.7 6 6 5 5 0 dual b/y bias 51.710373 0.824268 533.2573 1065.511 -0.0045 -4.2 0.5 6.0 9072.2 nuORFdb_human ENST00000345662.5_1_2:32288779-32289037:+ ENST00000345662.5_1_2:32288779-32289037:+ SPAST 5' Overlap uORF 5' Overlap uORF GN=SPAST 5' Overlap uORF E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep4_Slot2-5_1_3522 5mio-rep1 E_20221201_NO30_400nL_HLAc1_5e6_directIP_titration_rep1_Slot1-37_1_3502.15340.1499.2 2 4 1 14.37 7.72 6.68 6.65 92.4 6 1 1 26600.0 Hydrogen 38 (V) 9 RAVNEFSGW (T) 9 KAQTKCSGW R A V|N\E|F|S|G\W 66.7 6 6 4 4 0 dual b/y bias 52.987358 0.81412 533.2554 1065.511 -0.0085 -8.0 -1.0 6.0 9072.2 nuORFdb_human ENST00000345662.5_1_2:32288779-32289037:+ ENST00000345662.5_1_2:32288779-32289037:+ SPAST 5' Overlap uORF 5' Overlap uORF GN=SPAST 5' Overlap uORF E_20221201_NO30_400nL_HLAc1_5e6_directIP_titration_rep1_Slot1-37_1_3502 5mio-rep2 E_20221201_NO30_400nL_HLAc1_5e6_directIP_titration_rep2_Slot2-2_1_3510.15240.1506.2 2 2 1 10.9 4.92 5.05 5.98 79.3 6 1 1 7240.0 Hydrogen 38 (V) 9 RAVNEFSGW (T) 9 NFSWVNLGE R A V|N|E|F|S|G\W 66.7 6 6 5 5 0 dual b/y bias 52.639648 0.809838 533.2555 1065.511 -0.0065 -6.1 2.5999999999999996 6.0 9072.2 nuORFdb_human ENST00000345662.5_1_2:32288779-32289037:+ ENST00000345662.5_1_2:32288779-32289037:+ SPAST 5' Overlap uORF 5' Overlap uORF GN=SPAST 5' Overlap uORF E_20221201_NO30_400nL_HLAc1_5e6_directIP_titration_rep2_Slot2-2_1_3510 SCP E_20221201_NO30_400nL_HLAc1_4e7_PDAC_Slot2-6_1_3524.17617.1234.2 2 1 1 15.13 6.76 8.37 8.37 80.5 8 1 1 8940.0 M36m Hydrogen 28 (R) 11 RCCSFCAGmLL (-) 11 DTLnmYEDmLL R C\C\S F|C|A\G|m\L\L 63.6 7 8 3 3 0 b-ion bias Carbamidomethylation C:Carbamidomethylation,m:Oxidized methionine 60.861332 0.979621 695.7902 1374.579 15.9942 -0.5 1.9 9.75 4580.6 nuORFdb_human ENST00000440180.5_1_2:201994562-201994679:+ ENST00000440180.5_1_2:201994562-201994679:+ CFLAR Out-of-Frame Out-of-Frame GN=CFLAR Out-of-Frame E_20221201_NO30_400nL_HLAc1_4e7_PDAC_Slot2-6_1_3524 40mio-rep4 E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep4_Slot2-5_1_3522.5785.1613.3 3 1 1 10.45 8.56 1.18 1.9 86.8 6 2 2 3840.0 Hydrogen 18 (R) 11 REAAAAAAGRW (R) 11 ERVGGAAWRQ R E\A A\A|A|A|A/G R W 36.4 4 5 4 3 0 unbiased 19.985462 0.741967 377.1973 1129.586 -0.0097 -8.6 -3.8999999999999995 9.6 3691.1 nuORFdb_human ENST00000360768.3_1_7:65338278-65338380:+ ENST00000360768.3_1_7:65338278-65338380:+ VKORC1L1 5' Overlap uORF 5' Overlap uORF GN=VKORC1L1 5' Overlap uORF E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep4_Slot2-5_1_3522 40mio-rep3 E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep3_Slot2-5_1_3521.5524.1588.3 3 1 1 8.22 3.85 5.29 4.38 62.9 7 3 3 89600.0 Hydrogen 24 (R) 10 REENFRDRGF (R) 10 REESHLnRHF R/E/E|N\F R/D/R G-F 60.0 6 2 5 1 1 y-ion bias 19.086714 0.757935 442.5473 1325.634 -0.0081 -6.1 -1.5 6.19 4891.4 nuORFdb_human ENST00000469451.1_1_1:203830997-203832824:+ ENST00000469451.1_1_1:203830997-203832824:+ SNRPE 5' Overlap uORF 5' Overlap uORF GN=SNRPE 5' Overlap uORF E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep3_Slot2-5_1_3521 40mio-rep1 E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep1_Slot1-04_1_3496.15306.1308.2 2 2 1 12.85 10.4 0.02 2.45 80.6 5 1 1 19300.0 Hydrogen 5 (E) 10 RELENTAQLL (-) 10 ASAAAAAAAASGALL R E L E N\T|A\Q|L\L 50.0 5 5 2 2 0 b-ion bias 52.870869 0.934055 593.8227 1186.643 -0.0039 -3.3 -0.09999999999999964 4.53 1604.8 nuORFdb_human ENST00000496756.1_1_1:120254524-120254566:+ ENST00000496756.1_1_1:120254524-120254566:+ PHGDH 5' uORF 5' uORF GN=PHGDH 5' uORF E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep1_Slot1-04_1_3496 40mio-rep3 E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep3_Slot2-5_1_3521.14994.1336.2 2 1 1 12.66 10.58 2.14 2.08 86.4 5 1 1 7470.0 Hydrogen 5 (E) 10 RELENTAQLL (-) 10 InVnVNSAAGLL R E L E N|T\A\Q|L\L 50.0 5 5 2 2 0 b-ion bias 51.797291 0.916349 593.8232 1186.643 -0.0039 -3.3 1.2999999999999998 4.53 1604.8 nuORFdb_human ENST00000496756.1_1_1:120254524-120254566:+ ENST00000496756.1_1_1:120254524-120254566:+ PHGDH 5' uORF 5' uORF GN=PHGDH 5' uORF E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep3_Slot2-5_1_3521 40mio-rep4 E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep4_Slot2-5_1_3522.14985.1336.2 2 1 1 10.29 7.89 1.36 2.4 77.5 5 1 1 9580.0 Hydrogen 5 (E) 10 RELENTAQLL (-) 10 InVnVNSAAGLL R E L E N\T\A\Q|L\L 50.0 5 5 1 1 0 b-ion bias 51.751781 0.916557 593.8219 1186.643 -0.0059 -5.0 -0.2999999999999998 4.53 1604.8 nuORFdb_human ENST00000496756.1_1_1:120254524-120254566:+ ENST00000496756.1_1_1:120254524-120254566:+ PHGDH 5' uORF 5' uORF GN=PHGDH 5' uORF E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep4_Slot2-5_1_3522 40mio-rep1 E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep1_Slot1-04_1_3496.3989.1651.3 3 1 1 9.85 3.64 6.51 6.21 75.9 7 2 3 4450.0 Hydrogen 13 (E) 9 RENFLVKKT (-) 9 VLQRDFKKT R E/N\F|L|V\K/K\T 77.8 7 5 4 2 0 unbiased 13.790431 0.717623 378.8912 1134.663 -0.0045 -4.0 -0.7999999999999998 9.99 2438.8 nuORFdb_human ENST00000274030.10_1_4:120160402-120160465:+ ENST00000274030.10_1_4:120160402-120160465:+ USP53 5' uORF 5' uORF GN=USP53 5' uORF E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep1_Slot1-04_1_3496 40mio-rep1 E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep1_Slot1-04_1_3496.7231.1396.2 2 1 1 9.77 8.32 1.02 1.45 69.7 5 2 2 8120.0 Hydrogen 5 (A) 9 RENNNKIVF (A) 9 LNQWDVLVF R E N N\N\K|I|V\F 55.6 5 5 2 2 0 b-ion bias 24.986292 0.87884 567.3052 1133.606 -0.0035 -3.1 0.10000000000000009 8.75 6012.7 nuORFdb_human ENST00000299633.6_1_15:83876471-83876627:- ENST00000299633.6_1_15:83876471-83876627:- RP11-382A20.3 5' uORF 5' uORF GN=RP11-382A20.3 5' uORF E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep1_Slot1-04_1_3496 40mio-rep3 E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep3_Slot2-5_1_3521.6985.1416.2 2 1 1 7.46 6.11 1.62 1.35 64.9 5 2 2 8260.0 Hydrogen 5 (A) 9 RENNNKIVF (A) 9 KEPIQCLVF R E/N N N\K\I|V\F 44.4 4 4 2 1 0 b-ion bias 24.131983 0.866033 567.304 1133.606 -0.0055 -4.8 -0.20000000000000018 8.75 6012.7 nuORFdb_human ENST00000299633.6_1_15:83876471-83876627:- ENST00000299633.6_1_15:83876471-83876627:- RP11-382A20.3 5' uORF 5' uORF GN=RP11-382A20.3 5' uORF E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep3_Slot2-5_1_3521 40mio-rep1 E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep1_Slot1-04_1_3496.23342.1267.2 2 1 1 7.45 5.73 0.85 1.72 52.6 5 1 1 6110.0 Hydrogen 19 (Q) 9 REQEWLYLY (Q) 9 FPYSLAYYLY R E Q E\W\L|Y|L\Y 55.6 5 5 2 2 0 b-ion bias 80.636864 0.959669 650.3187 1299.637 -0.0061 -4.7 -1.5 4.53 5577.4 nuORFdb_human ENST00000490682.5_1_2:202105031-202105169:+ ENST00000490682.5_1_2:202105031-202105169:+ CASP8 5' uORF 5' uORF GN=CASP8 5' uORF E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep1_Slot1-04_1_3496 10mio-rep3 E_20221201_NO30_400nL_HLAc1_1e7_directIP_titration_rep3_Slot2-3_1_3514.8103.1510.2 2 1 1 7.75 6.19 1.56 1.56 65.2 5 1 1 3270.0 Hydrogen 39 (G) 9 REVGSAETW (I) 9 GTRnLGETW R E V\G S\A\E|T\W 44.4 4 5 1 1 0 b-ion bias 28.000114 0.806932 517.7456 1034.49 -0.0054 -5.2 1.0999999999999996 4.53 5690.5 smORF_v4 TFCP2_12:51566735-51566887:- TFCP2_12:51566735-51566887:- TFCP2 uORF 12:51566735-51566887:- 12 5' uORF GN=TFCP2 E_20221201_NO30_400nL_HLAc1_1e7_directIP_titration_rep3_Slot2-3_1_3514 40mio-rep1 E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep1_Slot1-04_1_3496.8321.1472.2 2 1 1 9.33 7.28 1.55 2.05 72.9 6 1 1 12400.0 Hydrogen 39 (G) 9 REVGSAETW (I) 9 GTRnLGETW R E V\G\S\A\E|T\W 66.7 6 6 1 1 0 b-ion bias 28.749409 0.831014 517.748 1034.49 -0.0014 -1.4 1.8000000000000003 4.53 5690.5 smORF_v4 TFCP2_12:51566735-51566887:- TFCP2_12:51566735-51566887:- TFCP2 uORF 12:51566735-51566887:- 12 5' uORF GN=TFCP2 E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep1_Slot1-04_1_3496 40mio-rep3 E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep3_Slot2-5_1_3521.8024.1492.2 2 1 1 9.04 6.46 1.5 2.58 74.8 6 1 1 8660.0 Hydrogen 39 (G) 9 REVGSAETW (I) 9 GTRnLGETW R E\V\G S\A\E|T\W 55.6 5 6 1 1 0 b-ion bias 27.72015 0.818601 517.7454 1034.49 -0.0074 -7.2 -2.6000000000000005 4.53 5690.5 smORF_v4 TFCP2_12:51566735-51566887:- TFCP2_12:51566735-51566887:- TFCP2 uORF 12:51566735-51566887:- 12 5' uORF GN=TFCP2 E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep3_Slot2-5_1_3521 40mio-rep4 E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep4_Slot2-5_1_3522.8035.1490.2 2 1 1 11.73 9.66 2.07 2.07 78.8 6 1 1 12700.0 Hydrogen 39 (G) 9 REVGSAETW (I) 9 GTRnLGETW R E V\G\S\A\E|T\W 66.7 6 6 1 1 0 b-ion bias 27.753296 0.819598 517.7446 1034.49 -0.0074 -7.2 -2.5 4.53 5690.5 smORF_v4 TFCP2_12:51566735-51566887:- TFCP2_12:51566735-51566887:- TFCP2 uORF 12:51566735-51566887:- 12 5' uORF GN=TFCP2 E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep4_Slot2-5_1_3522 Eva-50mio-3fr AL20210827_EV_HLA_PDAC_PANFRO413_2D_50e6_W632_classI_fxn3.19652.19652.2 2 2 1 14.03 9.64 7.75 4.39 87.0 6 1 1 20900000.0 Hydrogen 13 (N) 9 RFIQAHPQW (I) 9 ARAAGARGPAGW R\F I Q\A\H|P|Q\W 66.7 6 6 2 2 0 b-ion bias 0.998 98.1 37.08 100.0 0.0 591.8125 1182.617 0.0011 0.9 0.4 9.75 6122.2 nuORFdb_human ENST00000268712.7_1_17:16075166-16089874:- ENST00000268712.7_1_17:16075166-16089874:-|ENST00000460276.5_1_17:16075166-16075322:- NCOR1 Out-of-Frame Out-of-Frame GN=NCOR1 Out-of-Frame AL20210827_EV_HLA_PDAC_PANFRO413_2D_50e6_W632_classI_fxn3 Exploris_FAIMS VH20221207_MKP_FAIMS2022E_HLAc1_4e7_PDAC.31469.31469.2 2 2 1 12.71 7.03 7.54 5.68 82.6 6 1 1 38900000.0 Hydrogen 13 (N) 9 RFIQAHPQW (I) 9 ARAAGARGPAGW R\F I\Q A\H|P|Q\W 55.6 5 6 2 2 0 b-ion bias 0.994 100.0 60.97 22.81 -50.0 591.8107 1182.617 -0.0025 -2.1 0.5 9.75 6122.2 nuORFdb_human ENST00000268712.7_1_17:16075166-16089874:- ENST00000268712.7_1_17:16075166-16089874:-|ENST00000460276.5_1_17:16075166-16075322:- NCOR1 Out-of-Frame Out-of-Frame GN=NCOR1 Out-of-Frame VH20221207_MKP_FAIMS2022E_HLAc1_4e7_PDAC SCP E_20221201_NO30_400nL_HLAc1_4e7_PDAC_Slot2-6_1_3524.29394.432.1 1 1 1 7.61 6.37 0.13 1.24 76.1 5 1 1 195000.0 Hydrogen 49 (R) 8 RFLLAAQF (A) 8 FRLLMASK R/F|L|L|A A Q-F 62.5 5 3 4 3 1 dual b/y bias 101.554771 1.466281 965.5568 965.557 0.0003 0.3 2.6999999999999997 9.75 15629.8 nuORFdb_human ENST00000358268.6_2_21:40801131-40817638:- ENST00000358268.6_2_21:40801131-40817638:-|ENST00000438404.5_2_21:40816571-40817638:- LCA5L 5' uORF 5' uORF GN=LCA5L 5' uORF E_20221201_NO30_400nL_HLAc1_4e7_PDAC_Slot2-6_1_3524 Exploris AL20221201_MKP_PDAC_01.67477.67477.2 2 1 1 10.76 6.32 4.35 4.44 85.6 6 1 1 18300000.0 Hydrogen 110 (A) 10 RFMPELPLPW (-) 10 RCLSASVPLPW R F M\P E\L\P\L|P/W 50.0 5 5 2 1 0 b-ion bias 0.979 82.1 101.3 44.25 0.0 643.342 1285.676 0.0005 0.4 -0.4 6.0 13219.1 nuORFdb_human ENST00000325942.10_1_4:78978964-79158713:+ ENST00000325942.10_1_4:78978964-79158713:+ FRAS1 5' Overlap uORF 5' Overlap uORF GN=FRAS1 5' Overlap uORF AL20221201_MKP_PDAC_01 SCP E_20221201_NO30_400nL_HLAc1_4e7_PDAC_Slot2-6_1_3524.29725.1301.2 2 1 1 14.09 10.41 5.07 3.68 94.7 6 1 1 21700.0 Hydrogen 110 (A) 10 RFMPELPLPW (-) 10 RCLSASVPLPW R F M|P E\L|P|L|P\W 50.0 5 6 4 4 0 dual b/y bias 102.698326 0.938166 643.3408 1285.676 -0.0015 -1.1 1.2999999999999998 6.0 13219.1 nuORFdb_human ENST00000325942.10_1_4:78978964-79158713:+ ENST00000325942.10_1_4:78978964-79158713:+ FRAS1 5' Overlap uORF 5' Overlap uORF GN=FRAS1 5' Overlap uORF E_20221201_NO30_400nL_HLAc1_4e7_PDAC_Slot2-6_1_3524 Exploris AL20221201_MKP_PDAC_01.62742.62858.2 2 2 1 16.28 9.04 8.14 7.24 81.5 8 2 2 396000000.0 Hydrogen 37 (R) 9 RFSPPLRLF (-) 9 RAALAASGRIF R|F/S|P/P\L\R|L|F 100.0 9 6 6 4 0 dual b/y bias 0.999 94.1 94.8 100.0 0.0 566.8356 1132.663 0.0013 1.1 0.30000000000000004 12.0 4859.5 smORF_v4|nuORFdb_human PTEN_10:89623466-89623603:+ PTEN_10:89623466-89623603:+|ENST00000371953.7_1_10:89623465-89623600:+ PTEN uORF 10:89623466-89623603:+ 10 PTEN 5' uORF 5' uORF GN=PTEN AL20221201_MKP_PDAC_01 SCP E_20221201_NO30_400nL_HLAc1_4e7_PDAC_Slot2-6_1_3524.27997.1373.2 2 3 1 10.78 5.5 6.52 5.28 75.3 7 2 2 416000.0 Hydrogen 37 (R) 9 RFSPPLRLF (-) 9 RAALAASGRIF R/F/S|P/P L\R\L\F 77.8 7 4 4 1 0 unbiased 96.724472 0.893463 566.8328 1132.663 -0.0039 -3.4 -1.0 12.0 4859.5 smORF_v4|nuORFdb_human PTEN_10:89623466-89623603:+ PTEN_10:89623466-89623603:+|ENST00000371953.7_1_10:89623465-89623600:+ PTEN uORF 10:89623466-89623603:+ 10 PTEN 5' uORF 5' uORF GN=PTEN E_20221201_NO30_400nL_HLAc1_4e7_PDAC_Slot2-6_1_3524 Exploris_FAIMS VH20221207_MKP_FAIMS2022E_HLAc1_4e7_PDAC.63410.63410.2 2 1 1 15.52 6.17 9.75 9.35 74.7 8 2 2 74600000.0 Hydrogen 37 (R) 9 RFSPPLRLF (-) 9 RAALAASGRIF R|F|S|P|P\L\R|L/F 100.0 9 7 6 5 0 dual b/y bias 0.991 78.1 108.55 2.75 -50.0 566.8337 1132.663 -0.0024 -2.1 0.5 12.0 4859.5 smORF_v4|nuORFdb_human PTEN_10:89623466-89623603:+ PTEN_10:89623466-89623603:+|ENST00000371953.7_1_10:89623465-89623600:+ PTEN uORF 10:89623466-89623603:+ 10 PTEN 5' uORF 5' uORF GN=PTEN VH20221207_MKP_FAIMS2022E_HLAc1_4e7_PDAC 40mio-rep2 E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep2_Slot2-5_1_3520.9982.1334.2 2 2 1 9.14 6.44 4.94 2.7 60.5 5 2 2 17100.0 M47m Hydrogen 41 (K) 9 RINSFKmNW (R) 9 AAMLFVLmNW R I N\S F\K|m|N\W 44.4 4 5 2 2 0 b-ion bias m:Oxidized methionine 34.483028 0.917662 606.2995 1195.604 15.9866 -6.8 -1.8999999999999995 11.0 6578.4 nuORFdb_human ENST00000487475.5_1_3:184017076-184017693:+ ENST00000487475.5_1_3:184017076-184017693:+|ENST00000433010.5_1_3:184017407-184017693:+ PSMD2 ncRNA Retained Intron lncRNA GN=PSMD2 ncRNA Retained Intron E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep2_Slot2-5_1_3520 Eva-50mio-3fr AL20210827_EV_HLA_PDAC_PANFRO413_2D_50e6_W632_classI_fxn1.13721.13721.2 2 1 1 11.96 7.14 8.37 4.82 75.3 6 1 2 8060000.0 Hydrogen 28 (W) 9 RISDDRPNF (Q) 9 FDPNIRDSR R|I/S D|D|R|P N-F 55.6 5 4 5 4 1 dual b/y bias 0.992 93.6 26.68 94.07 0.0 560.2806 1119.554 -0.0002 -0.2 -0.30000000000000004 5.96 6483.0 nuORFdb_human ENST00000620860.4_1_1:45446712-45452201:- ENST00000620860.4_1_1:45446712-45452201:- EIF2B3 5' Overlap uORF 5' Overlap uORF GN=EIF2B3 5' Overlap uORF AL20210827_EV_HLA_PDAC_PANFRO413_2D_50e6_W632_classI_fxn1 Exploris AL20221201_MKP_PDAC_01.16946.16946.2 2 1 1 9.74 4.79 6.77 4.95 58.8 6 1 2 36100000.0 Hydrogen 28 (W) 9 RISDDRPNF (Q) 9 FDPNIRDSR R|I/S D|D|R|P N-F 55.6 5 4 5 4 1 dual b/y bias 0.861 100.0 30.07 100.0 0.0 560.2812 1119.554 0.001 0.9 0.09999999999999998 5.96 6483.0 nuORFdb_human ENST00000620860.4_1_1:45446712-45452201:- ENST00000620860.4_1_1:45446712-45452201:- EIF2B3 5' Overlap uORF 5' Overlap uORF GN=EIF2B3 5' Overlap uORF AL20221201_MKP_PDAC_01 Exploris_FAIMS VH20221207_MKP_FAIMS2022E_HLAc1_4e7_PDAC.22524.22524.2 2 1 1 10.72 7.05 7.02 3.67 64.5 7 1 2 14000000.0 Hydrogen 28 (W) 9 RISDDRPNF (Q) 9 FDPNIRDSR R/I/S\D|D|R|P N-F 77.8 7 4 5 3 1 dual b/y bias 0.946 75.7 46.87 120.0 -50.0 560.2781 1119.554 -0.0051 -4.6 -1.9999999999999996 5.96 6483.0 nuORFdb_human ENST00000620860.4_1_1:45446712-45452201:- ENST00000620860.4_1_1:45446712-45452201:- EIF2B3 5' Overlap uORF 5' Overlap uORF GN=EIF2B3 5' Overlap uORF VH20221207_MKP_FAIMS2022E_HLAc1_4e7_PDAC Exploris AL20221201_MKP_PDAC_01.9003.9003.3 3 1 1 7.59 3.06 5.07 4.54 60.1 5 2 2 26300000.0 Hydrogen 9 (A) 9 RLAPLRHAC (-) 9 RPHLRALAC R L A\P L|R\H|A|C 44.4 4 5 3 3 0 dual b/y bias Carbamidomethylation C:Carbamidomethylation, 0.982 69.9 17.27 50.94 0.0 365.2056 1093.605 -0.0024 -2.2 -3.0 12.0 1924.3 nuORFdb_human ENST00000269593.4_1_17:38599756-38599807:+ ENST00000269593.4_1_17:38599756-38599807:+ IGFBP4 5' uORF 5' uORF GN=IGFBP4 5' uORF AL20221201_MKP_PDAC_01 SCP E_20221201_NO30_400nL_HLAc1_4e7_PDAC_Slot2-6_1_3524.2972.1637.3 3 1 1 9.68 3.41 5.53 6.27 69.0 6 2 2 24400.0 Hydrogen 9 (A) 9 RLAPLRHAC (-) 9 NLPLRLAHC R L A|P/L/R|H\A\C 66.7 6 4 4 2 0 unbiased Carbamidomethylation C:Carbamidomethylation, 10.278365 0.726593 365.2047 1093.605 -0.0043 -3.9 -1.5 12.0 1924.3 nuORFdb_human ENST00000269593.4_1_17:38599756-38599807:+ ENST00000269593.4_1_17:38599756-38599807:+ IGFBP4 5' uORF 5' uORF GN=IGFBP4 5' uORF E_20221201_NO30_400nL_HLAc1_4e7_PDAC_Slot2-6_1_3524 Exploris_FAIMS VH20221207_MKP_FAIMS2022E_HLAc1_4e7_PDAC.11860.11860.3 3 1 1 11.3 4.81 11.3 6.49 62.1 6 2 2 2070000.0 Hydrogen 9 (A) 9 RLAPLRHAC (-) 9 RPHLRALAC R L\A\P L|R\H|A|C 55.6 5 6 3 3 0 dual b/y bias Carbamidomethylation C:Carbamidomethylation, 0.993 83.2 25.67 120.0 -50.0 365.2055 1093.605 -0.0029 -2.7 -0.10000000000000009 12.0 1924.3 nuORFdb_human ENST00000269593.4_1_17:38599756-38599807:+ ENST00000269593.4_1_17:38599756-38599807:+ IGFBP4 5' uORF 5' uORF GN=IGFBP4 5' uORF VH20221207_MKP_FAIMS2022E_HLAc1_4e7_PDAC 1mio-rep1 E_20221201_NO30_400nL_HLAc1_1e6_directIP_titration_rep1_Slot1-06_1_3498.11521.1478.2 2 4 1 17.2 11.59 6.17 5.61 89.0 7 1 1 13500.0 Hydrogen 15 (V) 9 RLASLQSEV (-) 9 GASLRLASEV R L/A|S\L|Q|S|E\V 77.8 7 6 5 4 0 dual b/y bias 39.797928 0.827656 501.7804 1002.558 -0.0051 -5.1 -0.1999999999999993 6.0 2480.8 smORF_v4|nuORFdb_human REPIN1_7:150065992-150066792:+ REPIN1_7:150065992-150066792:+|ENST00000397281.6_1_7:150065961-150066789:+|ENST00000487455.1_1_7:150065323-150066789:+ REPIN1 uORF 7:150065992-150066792:+ 7 REPIN1 5' Overlap uORF 5' Overlap uORF GN=REPIN1 E_20221201_NO30_400nL_HLAc1_1e6_directIP_titration_rep1_Slot1-06_1_3498 1mio-rep2 E_20221201_NO30_400nL_HLAc1_1e6_directIP_titration_rep3_Slot2-1_1_3508.11533.1502.2 2 1 1 9.87 7.23 3.34 2.64 81.0 5 1 1 4860.0 Hydrogen 15 (V) 9 RLASLQSEV (-) 9 GASLRLASEV R L A S/L|Q|S\E\V 55.6 5 4 3 2 0 unbiased 39.835094 0.812028 501.778 1002.558 -0.0091 -9.1 -0.7999999999999989 6.0 2480.8 smORF_v4|nuORFdb_human REPIN1_7:150065992-150066792:+ REPIN1_7:150065992-150066792:+|ENST00000397281.6_1_7:150065961-150066789:+|ENST00000487455.1_1_7:150065323-150066789:+ REPIN1 uORF 7:150065992-150066792:+ 7 REPIN1 5' Overlap uORF 5' Overlap uORF GN=REPIN1 E_20221201_NO30_400nL_HLAc1_1e6_directIP_titration_rep3_Slot2-1_1_3508 1mio-rep3 E_20221201_NO30_400nL_HLAc1_1e6_directIP_titration_rep4_Slot2-1_1_3509.11427.1498.2 2 1 1 13.56 10.07 4.12 3.49 87.0 7 1 1 3040.0 Hydrogen 15 (V) 9 RLASLQSEV (-) 9 GASLRLASEV R L/A/S\L\Q|S|E\V 77.8 7 5 4 2 0 unbiased 39.466381 0.814502 501.7774 1002.558 -0.0111 -11.1 -3.0999999999999996 6.0 2480.8 smORF_v4|nuORFdb_human REPIN1_7:150065992-150066792:+ REPIN1_7:150065992-150066792:+|ENST00000397281.6_1_7:150065961-150066789:+|ENST00000487455.1_1_7:150065323-150066789:+ REPIN1 uORF 7:150065992-150066792:+ 7 REPIN1 5' Overlap uORF 5' Overlap uORF GN=REPIN1 E_20221201_NO30_400nL_HLAc1_1e6_directIP_titration_rep4_Slot2-1_1_3509 10mio-rep1 E_20221201_NO30_400nL_HLAc1_1e7_directIP_titration_rep1_Slot2-3_1_3512.11185.1487.2 2 5 1 16.41 13.04 5.79 3.37 93.2 6 1 1 43500.0 Hydrogen 15 (V) 9 RLASLQSEV (-) 9 GASLRLASEV R L A\S\L|Q|S|E\V 66.7 6 6 3 3 0 dual b/y bias 38.644604 0.821538 501.7801 1002.558 -0.0051 -5.1 2.6000000000000005 6.0 2480.8 smORF_v4|nuORFdb_human REPIN1_7:150065992-150066792:+ REPIN1_7:150065992-150066792:+|ENST00000397281.6_1_7:150065961-150066789:+|ENST00000487455.1_1_7:150065323-150066789:+ REPIN1 uORF 7:150065992-150066792:+ 7 REPIN1 5' Overlap uORF 5' Overlap uORF GN=REPIN1 E_20221201_NO30_400nL_HLAc1_1e7_directIP_titration_rep1_Slot2-3_1_3512 10mio-rep2 E_20221201_NO30_400nL_HLAc1_1e7_directIP_titration_rep2_Slot2-3_1_3513.11167.1487.2 2 3 1 16.03 12.2 6.31 3.83 92.1 6 1 1 66500.0 Hydrogen 15 (V) 9 RLASLQSEV (-) 9 GASLRLASEV R L A|S|L\Q|S\E\V 66.7 6 6 3 3 0 dual b/y bias 38.580227 0.821603 501.7796 1002.558 -0.0051 -5.1 1.6000000000000005 6.0 2480.8 smORF_v4|nuORFdb_human REPIN1_7:150065992-150066792:+ REPIN1_7:150065992-150066792:+|ENST00000397281.6_1_7:150065961-150066789:+|ENST00000487455.1_1_7:150065323-150066789:+ REPIN1 uORF 7:150065992-150066792:+ 7 REPIN1 5' Overlap uORF 5' Overlap uORF GN=REPIN1 E_20221201_NO30_400nL_HLAc1_1e7_directIP_titration_rep2_Slot2-3_1_3513 10mio-rep3 E_20221201_NO30_400nL_HLAc1_1e7_directIP_titration_rep3_Slot2-3_1_3514.11134.1491.2 2 7 1 15.22 11.22 5.67 4.0 90.5 6 1 1 57000.0 Hydrogen 15 (V) 9 RLASLQSEV (-) 9 GASLRLASEV R L A|S\L|Q|S\E\V 66.7 6 6 3 3 0 dual b/y bias 38.466835 0.819279 501.7794 1002.558 -0.0071 -7.1 -0.7999999999999998 6.0 2480.8 smORF_v4|nuORFdb_human REPIN1_7:150065992-150066792:+ REPIN1_7:150065992-150066792:+|ENST00000397281.6_1_7:150065961-150066789:+|ENST00000487455.1_1_7:150065323-150066789:+ REPIN1 uORF 7:150065992-150066792:+ 7 REPIN1 5' Overlap uORF 5' Overlap uORF GN=REPIN1 E_20221201_NO30_400nL_HLAc1_1e7_directIP_titration_rep3_Slot2-3_1_3514 20mio-rep1 E_20221201_NO30_400nL_HLAc1_2e7_directIP_titration_rep1_Slot2-4_1_3515.11061.1492.2 2 3 1 16.59 12.56 5.13 4.03 92.3 6 1 1 54000.0 Hydrogen 15 (V) 9 RLASLQSEV (-) 9 GASLRLASEV R L\A S|L|Q|S|E\V 55.6 5 6 4 4 0 dual b/y bias 38.204269 0.818617 501.7774 1002.558 -0.0111 -11.1 -4.8999999999999995 6.0 2480.8 smORF_v4|nuORFdb_human REPIN1_7:150065992-150066792:+ REPIN1_7:150065992-150066792:+|ENST00000397281.6_1_7:150065961-150066789:+|ENST00000487455.1_1_7:150065323-150066789:+ REPIN1 uORF 7:150065992-150066792:+ 7 REPIN1 5' Overlap uORF 5' Overlap uORF GN=REPIN1 E_20221201_NO30_400nL_HLAc1_2e7_directIP_titration_rep1_Slot2-4_1_3515 20mio-rep2 E_20221201_NO30_400nL_HLAc1_2e7_directIP_titration_rep3_Slot2-4_1_3518.11233.1482.2 2 7 1 17.67 12.23 6.5 5.44 93.8 6 1 1 164000.0 Hydrogen 15 (V) 9 RLASLQSEV (-) 9 GASLRLASEV R L A|S|L|Q|S\E\V 66.7 6 6 4 4 0 dual b/y bias 38.80032 0.824664 501.7795 1002.558 -0.0071 -7.1 -0.7999999999999998 6.0 2480.8 smORF_v4|nuORFdb_human REPIN1_7:150065992-150066792:+ REPIN1_7:150065992-150066792:+|ENST00000397281.6_1_7:150065961-150066789:+|ENST00000487455.1_1_7:150065323-150066789:+ REPIN1 uORF 7:150065992-150066792:+ 7 REPIN1 5' Overlap uORF 5' Overlap uORF GN=REPIN1 E_20221201_NO30_400nL_HLAc1_2e7_directIP_titration_rep3_Slot2-4_1_3518 20mio-rep3 E_20221201_NO30_400nL_HLAc1_2e7_directIP_titration_rep4_Slot2-4_1_3519.11101.1481.2 2 4 1 18.26 13.83 6.6 4.44 95.6 6 1 1 79200.0 Hydrogen 15 (V) 9 RLASLQSEV (-) 9 GASLRLASEV R L A|S\L|Q|S\E\V 66.7 6 6 3 3 0 dual b/y bias 38.349415 0.825786 501.779 1002.558 -0.0071 -7.1 -1.2999999999999998 6.0 2480.8 smORF_v4|nuORFdb_human REPIN1_7:150065992-150066792:+ REPIN1_7:150065992-150066792:+|ENST00000397281.6_1_7:150065961-150066789:+|ENST00000487455.1_1_7:150065323-150066789:+ REPIN1 uORF 7:150065992-150066792:+ 7 REPIN1 5' Overlap uORF 5' Overlap uORF GN=REPIN1 E_20221201_NO30_400nL_HLAc1_2e7_directIP_titration_rep4_Slot2-4_1_3519 40mio-rep1 E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep1_Slot1-04_1_3496.11398.1453.2 2 1 1 12.85 9.89 4.09 2.96 76.6 6 1 1 172000.0 Hydrogen 15 (V) 9 RLASLQSEV (-) 9 GASLRLASEV R L A\S\L|Q|S\E\V 66.7 6 6 2 2 0 b-ion bias 39.373287 0.842857 501.7814 1002.558 -0.0031 -3.1 0.10000000000000009 6.0 2480.8 smORF_v4|nuORFdb_human REPIN1_7:150065992-150066792:+ REPIN1_7:150065992-150066792:+|ENST00000397281.6_1_7:150065961-150066789:+|ENST00000487455.1_1_7:150065323-150066789:+ REPIN1 uORF 7:150065992-150066792:+ 7 REPIN1 5' Overlap uORF 5' Overlap uORF GN=REPIN1 E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep1_Slot1-04_1_3496 40mio-rep2 E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep2_Slot2-5_1_3520.11059.1468.2 2 3 1 18.63 14.1 5.7 4.53 93.5 6 1 1 109000.0 Hydrogen 15 (V) 9 RLASLQSEV (-) 9 GASLRLASEV R L A/S|L|Q|S|E\V 66.7 6 5 5 4 0 dual b/y bias 38.203987 0.833848 501.7798 1002.558 -0.0051 -5.1 -0.1999999999999993 6.0 2480.8 smORF_v4|nuORFdb_human REPIN1_7:150065992-150066792:+ REPIN1_7:150065992-150066792:+|ENST00000397281.6_1_7:150065961-150066789:+|ENST00000487455.1_1_7:150065323-150066789:+ REPIN1 uORF 7:150065992-150066792:+ 7 REPIN1 5' Overlap uORF 5' Overlap uORF GN=REPIN1 E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep2_Slot2-5_1_3520 40mio-rep3 E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep3_Slot2-5_1_3521.11090.1472.2 2 2 1 17.41 13.0 5.27 4.41 93.7 6 1 1 254000.0 Hydrogen 15 (V) 9 RLASLQSEV (-) 9 GASLRLASEV R L A\S|L|Q|S\E\V 66.7 6 6 3 3 0 dual b/y bias 38.313602 0.8309 501.7794 1002.558 -0.0071 -7.1 -2.5 6.0 2480.8 smORF_v4|nuORFdb_human REPIN1_7:150065992-150066792:+ REPIN1_7:150065992-150066792:+|ENST00000397281.6_1_7:150065961-150066789:+|ENST00000487455.1_1_7:150065323-150066789:+ REPIN1 uORF 7:150065992-150066792:+ 7 REPIN1 5' Overlap uORF 5' Overlap uORF GN=REPIN1 E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep3_Slot2-5_1_3521 40mio-rep4 E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep4_Slot2-5_1_3522.11090.1472.2 2 3 1 18.63 13.21 6.04 5.43 93.3 6 1 1 115000.0 Hydrogen 15 (V) 9 RLASLQSEV (-) 9 GASLRLASEV R L A|S|L|Q|S|E\V 66.7 6 6 5 5 0 dual b/y bias 38.299011 0.831366 501.7796 1002.558 -0.0051 -5.1 -0.39999999999999947 6.0 2480.8 smORF_v4|nuORFdb_human REPIN1_7:150065992-150066792:+ REPIN1_7:150065992-150066792:+|ENST00000397281.6_1_7:150065961-150066789:+|ENST00000487455.1_1_7:150065323-150066789:+ REPIN1 uORF 7:150065992-150066792:+ 7 REPIN1 5' Overlap uORF 5' Overlap uORF GN=REPIN1 E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep4_Slot2-5_1_3522 5mio-rep1 E_20221201_NO30_400nL_HLAc1_5e6_directIP_titration_rep1_Slot1-37_1_3502.11432.1483.2 2 4 1 15.36 10.83 5.99 4.52 92.5 6 1 1 23800.0 Hydrogen 15 (V) 9 RLASLQSEV (-) 9 GASLRLASEV R L A\S|L|Q|S\E\V 66.7 6 6 3 3 0 dual b/y bias 39.491203 0.824163 501.7789 1002.558 -0.0071 -7.1 -0.09999999999999964 6.0 2480.8 smORF_v4|nuORFdb_human REPIN1_7:150065992-150066792:+ REPIN1_7:150065992-150066792:+|ENST00000397281.6_1_7:150065961-150066789:+|ENST00000487455.1_1_7:150065323-150066789:+ REPIN1 uORF 7:150065992-150066792:+ 7 REPIN1 5' Overlap uORF 5' Overlap uORF GN=REPIN1 E_20221201_NO30_400nL_HLAc1_5e6_directIP_titration_rep1_Slot1-37_1_3502 5mio-rep2 E_20221201_NO30_400nL_HLAc1_5e6_directIP_titration_rep2_Slot2-2_1_3510.11380.1496.2 2 2 1 13.38 8.98 3.88 4.4 87.9 6 1 1 11300.0 Hydrogen 15 (V) 9 RLASLQSEV (-) 9 GASLRLASEV R L/A|S L\Q|S\E\V 55.6 5 5 3 2 0 unbiased 39.306789 0.816233 501.7776 1002.558 -0.0091 -9.1 -0.40000000000000036 6.0 2480.8 smORF_v4|nuORFdb_human REPIN1_7:150065992-150066792:+ REPIN1_7:150065992-150066792:+|ENST00000397281.6_1_7:150065961-150066789:+|ENST00000487455.1_1_7:150065323-150066789:+ REPIN1 uORF 7:150065992-150066792:+ 7 REPIN1 5' Overlap uORF 5' Overlap uORF GN=REPIN1 E_20221201_NO30_400nL_HLAc1_5e6_directIP_titration_rep2_Slot2-2_1_3510 5mio-rep3 E_20221201_NO30_400nL_HLAc1_5e6_directIP_titration_rep3_Slot2-2_1_3511.11413.1503.2 2 3 1 13.39 9.92 3.97 3.47 88.8 6 1 1 20800.0 Hydrogen 15 (V) 9 RLASLQSEV (-) 9 GASLRLASEV R L A\S\L|Q\S|E\V 66.7 6 6 2 2 0 b-ion bias 39.428963 0.811577 501.7783 1002.558 -0.0091 -9.1 -0.9000000000000004 6.0 2480.8 smORF_v4|nuORFdb_human REPIN1_7:150065992-150066792:+ REPIN1_7:150065992-150066792:+|ENST00000397281.6_1_7:150065961-150066789:+|ENST00000487455.1_1_7:150065323-150066789:+ REPIN1 uORF 7:150065992-150066792:+ 7 REPIN1 5' Overlap uORF 5' Overlap uORF GN=REPIN1 E_20221201_NO30_400nL_HLAc1_5e6_directIP_titration_rep3_Slot2-2_1_3511 40mio-rep3 E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep3_Slot2-5_1_3521.6434.1450.2 2 1 1 7.13 5.89 2.69 1.24 65.1 6 2 2 7380.0 Hydrogen 68 (L) 9 RLATLKSTV (-) 9 VSRVLKSTV R L A\T\L\K\S\T\V 66.7 6 6 0 0 0 b-ion bias 22.22934 0.844913 494.8094 988.615 -0.0042 -4.3 0.2999999999999998 11.0 8550.0 nuORFdb_human ENST00000483373.5_1_3:142315288-142364209:+ ENST00000483373.5_1_3:142315288-142364209:+|ENST00000495744.5_1_3:142315288-142364209:+ PLS1 5' uORF 5' uORF GN=PLS1 5' uORF E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep3_Slot2-5_1_3521 40mio-rep4 E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep4_Slot2-5_1_3522.6446.1450.2 2 1 1 7.77 6.67 4.21 1.11 64.4 5 2 2 7250.0 Hydrogen 68 (L) 9 RLATLKSTV (-) 9 VSRVLKSTV R L A\T L|K|S\T\V 44.4 4 5 2 2 0 b-ion bias 22.266872 0.844991 494.808 988.615 -0.0062 -6.3 -1.5999999999999996 11.0 8550.0 nuORFdb_human ENST00000483373.5_1_3:142315288-142364209:+ ENST00000483373.5_1_3:142315288-142364209:+|ENST00000495744.5_1_3:142315288-142364209:+ PLS1 5' uORF 5' uORF GN=PLS1 5' uORF E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep4_Slot2-5_1_3522 Exploris_FAIMS VH20221207_MKP_FAIMS2022E_HLAc1_4e7_PDAC.25175.25175.2 2 1 1 13.35 8.53 3.88 4.82 79.3 6 1 2 2670000.0 Hydrogen 25 (L) 9 RLPSARPSF (W) 9 KWPRLPSF R|L|P S A\R|P/S|F 66.7 6 5 5 4 0 dual b/y bias 0.97 87.3 51.47 120.0 -70.0 515.7922 1030.579 -0.0022 -2.1 0.5 12.0 8430.4 nuORFdb_human ENST00000539294.5_1_8:64081202-64081433:+ ENST00000539294.5_1_8:64081202-64081433:+ YTHDF3 5' uORF 5' uORF GN=YTHDF3 5' uORF VH20221207_MKP_FAIMS2022E_HLAc1_4e7_PDAC 20mio-rep1 E_20221201_NO30_400nL_HLAc1_2e7_directIP_titration_rep1_Slot2-4_1_3515.20150.1384.2 2 3 1 14.21 3.4 11.32 10.82 82.2 8 1 1 15500.0 Hydrogen 190 (Q) 11 RLVDLSGGWDA (L) 11 DRRLAWWDA R L V\D|L\S\G\G|W\D\A 72.7 8 8 2 2 0 b-ion bias 69.586685 0.886146 594.7978 1188.601 -0.012 -10.1 -3.8999999999999995 4.21 29890.7 smORF_v4|nuORFdb_human SRCAP_16:30709299-30715403:+ SRCAP_16:30709299-30715403:+|ENST00000411466.6_3_16:30709553-30715400:+|ENST00000262518.8_1_16:30710550-30715400:+ SRCAP uORF 16:30709299-30715403:+ 16 SRCAP 5' Overlap uORF 5' Overlap uORF GN=SRCAP E_20221201_NO30_400nL_HLAc1_2e7_directIP_titration_rep1_Slot2-4_1_3515 20mio-rep2 E_20221201_NO30_400nL_HLAc1_2e7_directIP_titration_rep3_Slot2-4_1_3518.20183.1387.2 2 1 1 12.26 4.13 7.78 8.13 85.6 7 1 1 5160.0 Hydrogen 190 (Q) 11 RLVDLSGGWDA (L) 11 LKALQDEWDA R L V D\L\S\G\G|W\D\A 63.6 7 7 1 1 0 b-ion bias 69.71711 0.884718 594.7998 1188.601 -0.008 -6.8 -0.5 4.21 29890.7 smORF_v4|nuORFdb_human SRCAP_16:30709299-30715403:+ SRCAP_16:30709299-30715403:+|ENST00000411466.6_3_16:30709553-30715400:+|ENST00000262518.8_1_16:30710550-30715400:+ SRCAP uORF 16:30709299-30715403:+ 16 SRCAP 5' Overlap uORF 5' Overlap uORF GN=SRCAP E_20221201_NO30_400nL_HLAc1_2e7_directIP_titration_rep3_Slot2-4_1_3518 20mio-rep3 E_20221201_NO30_400nL_HLAc1_2e7_directIP_titration_rep4_Slot2-4_1_3519.20197.1378.2 2 1 1 11.67 4.73 6.91 6.95 84.2 7 1 1 4810.0 Hydrogen 190 (Q) 11 RLVDLSGGWDA (L) 11 nWKAIIEGADA R L V\D L\S|G\G\W\D\A 54.5 6 7 1 1 0 b-ion bias 69.755409 0.890123 594.8005 1188.601 -0.008 -6.8 -1.0 4.21 29890.7 smORF_v4|nuORFdb_human SRCAP_16:30709299-30715403:+ SRCAP_16:30709299-30715403:+|ENST00000411466.6_3_16:30709553-30715400:+|ENST00000262518.8_1_16:30710550-30715400:+ SRCAP uORF 16:30709299-30715403:+ 16 SRCAP 5' Overlap uORF 5' Overlap uORF GN=SRCAP E_20221201_NO30_400nL_HLAc1_2e7_directIP_titration_rep4_Slot2-4_1_3519 40mio-rep1 E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep1_Slot1-04_1_3496.20422.1350.2 2 1 1 13.72 3.9 10.4 9.82 81.8 8 1 1 7500.0 Hydrogen 190 (Q) 11 RLVDLSGGWDA (L) 11 nLSARVVGGEDA R L V|D\L\S|G\G\W\D\A 72.7 8 8 2 2 0 b-ion bias 70.547638 0.907572 594.8024 1188.601 -0.004 -3.4 -0.19999999999999973 4.21 29890.7 smORF_v4|nuORFdb_human SRCAP_16:30709299-30715403:+ SRCAP_16:30709299-30715403:+|ENST00000411466.6_3_16:30709553-30715400:+|ENST00000262518.8_1_16:30710550-30715400:+ SRCAP uORF 16:30709299-30715403:+ 16 SRCAP 5' Overlap uORF 5' Overlap uORF GN=SRCAP E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep1_Slot1-04_1_3496 40mio-rep2 E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep2_Slot2-5_1_3520.20184.1367.2 2 3 1 12.83 2.91 9.69 9.92 79.9 8 1 1 18300.0 Hydrogen 190 (Q) 11 RLVDLSGGWDA (L) 11 GRGARRSGEGW R L V|D\L\S|G\G\W\D\A 72.7 8 8 2 2 0 b-ion bias 69.726067 0.896998 594.8011 1188.601 -0.006 -5.1 -0.1999999999999993 4.21 29890.7 smORF_v4|nuORFdb_human SRCAP_16:30709299-30715403:+ SRCAP_16:30709299-30715403:+|ENST00000411466.6_3_16:30709553-30715400:+|ENST00000262518.8_1_16:30710550-30715400:+ SRCAP uORF 16:30709299-30715403:+ 16 SRCAP 5' Overlap uORF 5' Overlap uORF GN=SRCAP E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep2_Slot2-5_1_3520 40mio-rep3 E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep3_Slot2-5_1_3521.20218.1372.2 2 2 1 10.81 2.49 8.28 8.32 74.4 8 1 1 10300.0 Hydrogen 190 (Q) 11 RLVDLSGGWDA (L) 11 DNWSRGARRA R L V\D\L\S\G\G\W\D\A 72.7 8 8 0 0 0 b-ion bias 69.842667 0.894077 594.8002 1188.601 -0.008 -6.8 -2.2 4.21 29890.7 smORF_v4|nuORFdb_human SRCAP_16:30709299-30715403:+ SRCAP_16:30709299-30715403:+|ENST00000411466.6_3_16:30709553-30715400:+|ENST00000262518.8_1_16:30710550-30715400:+ SRCAP uORF 16:30709299-30715403:+ 16 SRCAP 5' Overlap uORF 5' Overlap uORF GN=SRCAP E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep3_Slot2-5_1_3521 40mio-rep4 E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep4_Slot2-5_1_3522.20208.1371.2 2 3 1 12.58 2.77 10.32 9.81 75.8 8 1 1 14300.0 Hydrogen 190 (Q) 11 RLVDLSGGWDA (L) 11 DRRLAWWDA R L V\D|L\S\G\G|W\D\A 72.7 8 8 2 2 0 b-ion bias 69.791069 0.894393 594.7999 1188.601 -0.008 -6.8 -2.0999999999999996 4.21 29890.7 smORF_v4|nuORFdb_human SRCAP_16:30709299-30715403:+ SRCAP_16:30709299-30715403:+|ENST00000411466.6_3_16:30709553-30715400:+|ENST00000262518.8_1_16:30710550-30715400:+ SRCAP uORF 16:30709299-30715403:+ 16 SRCAP 5' Overlap uORF 5' Overlap uORF GN=SRCAP E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep4_Slot2-5_1_3522 40mio-rep4 E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep4_Slot2-5_1_3522.8359.1601.3 3 1 1 9.76 4.76 4.14 5.0 81.4 6 2 2 3310.0 M72m Hydrogen 71 (S) 9 RmSTAVYRW (-) 9 MYTKVAVNME R m\S T|A|V|Y/R\W 55.6 5 5 4 3 0 dual b/y bias m:Oxidized methionine 28.872066 0.749671 395.8631 1169.588 15.986 -7.5 -2.8 10.84 9391.9 nuORFdb_human ENST00000264010.8_1_16:67605120-67644919:+ ENST00000264010.8_1_16:67605120-67644919:+ CTCF 5' Overlap uORF 5' Overlap uORF GN=CTCF 5' Overlap uORF E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep4_Slot2-5_1_3522 Exploris_FAIMS VH20221207_MKP_FAIMS2022E_HLAc1_4e7_PDAC.24158.24158.2 2 1 1 10.87 9.41 1.71 1.46 69.7 6 1 2 1560000.0 Hydrogen 34 (L) 8 RPAATLRL (S) 8 RPDKLRL R|P/A/A T\L\R\L 75.0 6 4 3 1 0 unbiased 1.0 64.7 49.68 120.0 -70.0 449.2839 897.563 -0.0023 -2.6 0.0 12.0 5346.2 nuORFdb_human ENST00000397901.7_1_16:89712324-89712471:- ENST00000397901.7_1_16:89712324-89712471:- CHMP1A 3' dORF 3' dORF GN=CHMP1A 3' dORF VH20221207_MKP_FAIMS2022E_HLAc1_4e7_PDAC SCP E_20221201_NO30_400nL_HLAc1_4e7_PDAC_Slot2-6_1_3524.2209.1652.3 3 2 1 7.06 1.94 7.06 5.12 51.8 6 1 3 9040.0 Hydrogen 2 (L) 9 RPAEKKNYL (F) 9 SHKTLYRTL R P A\E|K\K/N|Y\L 66.7 6 5 3 2 0 unbiased 7.6414 0.717403 373.5476 1118.632 -0.0022 -2.0 0.3999999999999999 9.7 6184.9 nuORFdb_human ENST00000327892.12_1_6:30688201-30690330:+ ENST00000327892.12_1_6:30688201-30690330:+ TUBB 5' Overlap uORF 5' Overlap uORF GN=TUBB 5' Overlap uORF E_20221201_NO30_400nL_HLAc1_4e7_PDAC_Slot2-6_1_3524 SCP E_20221201_NO30_400nL_HLAc1_4e7_PDAC_Slot2-6_1_3524.2584.1427.2 2 3 1 12.44 8.05 5.0 4.4 75.2 7 1 2 26000.0 Hydrogen 44 (S) 9 RPHKDSGLL (-) 9 PRGVHGSSLL R P|H\K\D\S\G|L\L 77.8 7 7 2 2 0 b-ion bias 8.936276 0.859608 511.79 1022.574 -0.0014 -1.4 1.0 8.76 5933.1 nuORFdb_human ENST00000342444.8_1_10:73970511-73973025:- ENST00000342444.8_1_10:73970511-73973025:- ASCC1 Out-of-Frame Out-of-Frame GN=ASCC1 Out-of-Frame E_20221201_NO30_400nL_HLAc1_4e7_PDAC_Slot2-6_1_3524 SCP E_20221201_NO30_400nL_HLAc1_4e7_PDAC_Slot2-6_1_3524.3446.1678.3 3 3 1 11.4 5.53 4.93 5.87 72.5 6 0 1 40600.0 Hydrogen 49 (R) 8 RPHLQSHL (-) 8 TPRELSTAL R P|H|L|Q|S\H\L 75.0 6 6 4 4 0 dual b/y bias 11.917032 0.701036 329.8521 987.548 -0.0068 -6.9 -4.5 9.76 6630.6 nuORFdb_human ENST00000397574.6_1_11:615555-615988:- ENST00000397574.6_1_11:615555-615988:- IRF7 5' uORF 5' uORF GN=IRF7 5' uORF E_20221201_NO30_400nL_HLAc1_4e7_PDAC_Slot2-6_1_3524 SCP E_20221201_NO30_400nL_HLAc1_4e7_PDAC_Slot2-6_1_3524.5071.1455.2 2 1 1 12.91 6.69 3.99 6.23 80.8 6 0 1 6880.0 Hydrogen 12 (G) 10 RPHSGIGSAL (A) 10 SRPHINSAL R P|H S G|I|G\S\A\L 50.0 5 6 3 3 0 b-ion bias 17.527515 0.841614 497.7729 994.543 -0.0041 -4.2 -1.8000000000000003 9.75 7768.6 nuORFdb_human ENST00000479101.1_1_1:44707597-44713662:- ENST00000479101.1_1_1:44707597-44713662:- ERI3 ncRNA Processed Transcript lncRNA GN=ERI3 ncRNA Processed Transcript E_20221201_NO30_400nL_HLAc1_4e7_PDAC_Slot2-6_1_3524 Eva-50mio-3fr AL20210827_EV_HLA_PDAC_PANFRO413_2D_50e6_W632_classI_fxn3.15202.15202.2 2 1 1 11.44 10.03 4.14 1.41 77.4 5 1 2 7290000.0 Hydrogen 21 (K) 9 RPRTSFLAA (S) 9 RFLRnGLAA R|P R T S\F\L|A\A 55.6 5 5 2 2 0 b-ion bias 0.963 98.5 29.17 56.0 0.0 509.7936 1018.579 0.0006 0.6 0.09999999999999998 12.0 3976.5 nuORFdb_human ENST00000319264.3_1_9:12775157-12775265:+ ENST00000319264.3_1_9:12775157-12775265:+ LURAP1L 5' uORF 5' uORF GN=LURAP1L 5' uORF AL20210827_EV_HLA_PDAC_PANFRO413_2D_50e6_W632_classI_fxn3 Exploris_FAIMS VH20221207_MKP_FAIMS2022E_HLAc1_4e7_PDAC.22948.23069.2 2 1 1 9.56 7.99 2.49 1.57 74.0 5 1 2 44800000.0 Hydrogen 21 (K) 9 RPRTSFLAA (S) 9 RFLRnGLAA R|P R T S\F\L|A\A 55.6 5 5 2 2 0 b-ion bias 0.977 88.9 47.67 21.28 -50.0 509.7911 1018.579 -0.0044 -4.3 -1.6999999999999997 12.0 3976.5 nuORFdb_human ENST00000319264.3_1_9:12775157-12775265:+ ENST00000319264.3_1_9:12775157-12775265:+ LURAP1L 5' uORF 5' uORF GN=LURAP1L 5' uORF VH20221207_MKP_FAIMS2022E_HLAc1_4e7_PDAC Exploris_FAIMS VH20221207_MKP_FAIMS2022E_HLAc1_4e7_PDAC.26690.26690.2 2 1 1 8.66 5.81 2.86 2.86 66.5 6 1 2 7120000.0 Hydrogen 35 (A) 10 RPSPVRVAAL (-) 10 RIFLYQIL R|P/S/P V R\V A/A|L 50.0 5 3 5 2 0 unbiased 0.998 80.4 53.78 51.14 -70.0 533.3288 1065.653 -0.0025 -2.3 0.30000000000000027 12.0 4828.7 nuORFdb_human ENST00000318809.11_1_19:41120140-41120272:+ ENST00000318809.11_1_19:41120140-41120272:+|ENST00000204005.13_1_19:41119864-41120272:+ LTBP4 ncRNA Retained Intron lncRNA GN=LTBP4 ncRNA Retained Intron VH20221207_MKP_FAIMS2022E_HLAc1_4e7_PDAC SCP E_20221201_NO30_400nL_HLAc1_4e7_PDAC_Slot2-6_1_3524.4297.1689.3 3 1 1 19.4 6.33 13.87 13.07 83.4 7 1 2 8160.0 Hydrogen 24 (L) 11 RPVGTVQRSEY (-) 11 RPSVGLVQSWY R P/V G T|V|Q|R|S|E\Y 54.5 6 6 6 5 0 dual b/y bias 14.855365 0.693604 431.2279 1291.675 -0.0059 -4.6 -2.1999999999999997 8.75 3909.5 smORF_v4|nuORFdb_human LZIC_1:10002691-10002792:- LZIC_1:10002691-10002792:-|ENST00000377223.5_1_1:10002691-10003325:-|ENST00000377213.1_1_1:10002691-10003464:- LZIC uORF 1:10002691-10002792:- 1 LZIC 5' uORF 5' uORF GN=LZIC E_20221201_NO30_400nL_HLAc1_4e7_PDAC_Slot2-6_1_3524 SCP E_20221201_NO30_400nL_HLAc1_4e7_PDAC_Slot2-6_1_3524.9486.1532.2 2 2 1 15.41 9.15 6.26 6.26 82.3 7 0 1 32300.0 Hydrogen 63 (R) 9 RPWSGPSSV (S) 9 CVRPAPSSV R P|W\S|G|P\S\S\V 77.8 7 7 3 3 0 dual b/y bias 32.773201 0.793521 486.7467 972.49 -0.003 -3.1 -0.7000000000000002 9.75 7925.7 nuORFdb_human ENST00000479540.5_1_20:62204085-62204322:- ENST00000479540.5_1_20:62204085-62204322:- HELZ2 ncRNA Processed Transcript lncRNA GN=HELZ2 ncRNA Processed Transcript E_20221201_NO30_400nL_HLAc1_4e7_PDAC_Slot2-6_1_3524 SCP E_20221201_NO30_400nL_HLAc1_4e7_PDAC_Slot2-6_1_3524.15593.1360.2 2 2 1 9.37 6.06 2.98 3.32 72.0 6 0 1 192000.0 Hydrogen 36 (S) 9 RPWSTTLQY (L) 9 PPAPPPGDLQY R P W\S\T\T\L|Q\Y 66.7 6 6 1 1 0 b-ion bias 53.8703 0.901348 576.296 1151.584 0.0003 0.3 2.6999999999999997 8.75 13833.7 nuORFdb_human ENST00000278893.11_1_11:62469959-62473679:- ENST00000278893.11_1_11:62469959-62473679:- BSCL2 Out-of-Frame Out-of-Frame GN=BSCL2 Out-of-Frame E_20221201_NO30_400nL_HLAc1_4e7_PDAC_Slot2-6_1_3524 40mio-rep1 E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep1_Slot1-04_1_3496.7407.1473.2 2 2 1 9.28 2.84 9.28 63.7 5 1 1 13800.0 C31c Hydrogen 25 (L) 9 RSAAVTcTF (Q) 9 R S A A\V|T|c|T\F 55.6 5 5 3 3 0 dual b/y bias c:Cysteinylation 25.593756 0.830814 537.7369 1012.488 61.9787 -3.7 -0.5 9.75 4198.6 nuORFdb_human ENST00000568470.5_1_16:66968788-66968911:+ ENST00000568470.5_1_16:66968788-66968911:+ CES2 5' uORF 5' uORF GN=CES2 5' uORF E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep1_Slot1-04_1_3496 40mio-rep3 E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep3_Slot2-5_1_3521.12952.1597.3 3 1 1 11.0 2.14 7.92 8.86 70.6 7 2 2 5570.0 Hydrogen 42 (C) 10 RSFADSYRQW (K) 10 RDAFSSVCLNF R S F\A|D|S|Y/R\Q\W 70.0 7 6 4 3 0 unbiased 44.746838 0.751985 439.2078 1315.618 -0.0084 -6.4 -1.8000000000000007 8.75 8502.5 nuORFdb_human ENST00000534610.5_1_11:46639304-46666898:+ ENST00000534610.5_1_11:46639304-46666898:+ ATG13 5' Overlap uORF 5' Overlap uORF GN=ATG13 5' Overlap uORF E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep3_Slot2-5_1_3521 40mio-rep3 E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep3_Slot2-5_1_3521.8367.1598.3 3 2 1 7.9 2.87 4.75 5.04 56.6 7 2 2 12400.0 Hydrogen 13 (T) 10 RSFPKASSSW (-) 10 WSVKPASSSFG R S F\P\K\A\S\S|S\W 70.0 7 7 1 1 0 b-ion bias 28.90633 0.751748 384.8615 1152.58 -0.0082 -7.1 -2.5 11.0 2564.9 nuORFdb_human ENST00000261735.3_1_12:112451375-112457590:+ ENST00000261735.3_1_12:112451375-112457590:+ ERP29 Out-of-Frame Out-of-Frame GN=ERP29 Out-of-Frame E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep3_Slot2-5_1_3521 40mio-rep3 E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep3_Slot2-5_1_3521.6317.1419.2 2 1 1 11.3 5.73 5.68 5.57 74.8 7 1 1 11500.0 Hydrogen 215 (A) 11 RSLGAAGSGHW (A) 11 LSRDERHW R/S L G A\A\G\S|G|H\W 63.6 7 6 3 2 0 b-ion bias 21.825521 0.864299 549.7737 1098.544 -0.0032 -2.9 1.6999999999999997 9.75 28799.4 nuORFdb_human ENST00000528558.1_1_8:145677565-145687797:- ENST00000528558.1_1_8:145677565-145687797:- CYHR1 ncRNA Retained Intron lncRNA GN=CYHR1 ncRNA Retained Intron E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep3_Slot2-5_1_3521 40mio-rep1 E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep1_Slot1-04_1_3496.7973.1442.2 2 1 1 12.68 10.14 4.7 2.53 83.7 5 1 1 9410.0 Hydrogen 11 (G) 10 RSSSSMGTSW (F) 10 DNKTSmTSW R S S S S|M|G|T|S\W 50.0 5 5 4 4 0 dual b/y bias 27.547619 0.850128 543.2361 1085.468 -0.0033 -3.0 0.20000000000000018 9.75 4375.9 nuORFdb_human ENST00000465738.1_1_7:99697321-99697695:- ENST00000465738.1_1_7:99697321-99697695:- MCM7 ncRNA Retained Intron lncRNA GN=MCM7 ncRNA Retained Intron E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep1_Slot1-04_1_3496 40mio-rep2 E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep2_Slot2-5_1_3520.7695.1462.2 2 1 1 12.11 7.88 4.17 4.24 82.7 6 1 1 9330.0 Hydrogen 11 (G) 10 RSSSSMGTSW (F) 10 TGESGNFTSW R S S\S S|M|G|T|S\W 50.0 5 6 4 4 0 dual b/y bias 26.587658 0.837426 543.2348 1085.468 -0.0053 -4.9 0.0 9.75 4375.9 nuORFdb_human ENST00000465738.1_1_7:99697321-99697695:- ENST00000465738.1_1_7:99697321-99697695:- MCM7 ncRNA Retained Intron lncRNA GN=MCM7 ncRNA Retained Intron E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep2_Slot2-5_1_3520 40mio-rep3 E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep3_Slot2-5_1_3521.7712.1465.2 2 2 1 13.93 8.98 5.72 4.95 87.8 6 1 1 16000.0 Hydrogen 11 (G) 10 RSSSSMGTSW (F) 10 SAASTSASMSW R S S S/S|M|G|T|S\W 60.0 6 5 5 4 0 dual b/y bias 26.642805 0.835695 543.235 1085.468 -0.0053 -4.9 -0.3000000000000007 9.75 4375.9 nuORFdb_human ENST00000465738.1_1_7:99697321-99697695:- ENST00000465738.1_1_7:99697321-99697695:- MCM7 ncRNA Retained Intron lncRNA GN=MCM7 ncRNA Retained Intron E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep3_Slot2-5_1_3521 40mio-rep4 E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep4_Slot2-5_1_3522.7718.1465.2 2 1 1 13.37 10.01 5.02 3.37 85.3 6 1 1 7360.0 Hydrogen 11 (G) 10 RSSSSMGTSW (F) 10 SAASTSASMSW R S S S/S|M|G|T|S\W 60.0 6 5 5 4 0 dual b/y bias 26.659184 0.83568 543.2337 1085.468 -0.0073 -6.7 -2.0 9.75 4375.9 nuORFdb_human ENST00000465738.1_1_7:99697321-99697695:- ENST00000465738.1_1_7:99697321-99697695:- MCM7 ncRNA Retained Intron lncRNA GN=MCM7 ncRNA Retained Intron E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep4_Slot2-5_1_3522 20mio-rep1 E_20221201_NO30_400nL_HLAc1_2e7_directIP_titration_rep1_Slot2-4_1_3515.4015.1490.2 2 1 1 9.83 6.13 4.09 3.7 69.2 6 1 1 3420.0 M16m Hydrogen 11 (G) 10 RSSSSmGTSW (F) 10 SAASTSASmSW R S S S/S|m\G|T|S\W 60.0 6 5 4 3 0 unbiased m:Oxidized methionine 13.877336 0.819845 551.2326 1085.468 15.9907 -3.8 2.4000000000000004 9.75 4375.9 nuORFdb_human ENST00000465738.1_1_7:99697321-99697695:- ENST00000465738.1_1_7:99697321-99697695:- MCM7 ncRNA Retained Intron lncRNA GN=MCM7 ncRNA Retained Intron E_20221201_NO30_400nL_HLAc1_2e7_directIP_titration_rep1_Slot2-4_1_3515 20mio-rep2 E_20221201_NO30_400nL_HLAc1_2e7_directIP_titration_rep3_Slot2-4_1_3518.3967.1493.2 2 1 1 11.41 8.57 4.79 2.84 70.6 5 1 1 3080.0 M16m Hydrogen 11 (G) 10 RSSSSmGTSW (F) 10 SAASTSASmSW R S S S/S|m G\T|S\W 40.0 4 4 3 2 0 unbiased m:Oxidized methionine 13.714213 0.817613 551.2319 1085.468 15.9887 -5.7 0.5999999999999996 9.75 4375.9 nuORFdb_human ENST00000465738.1_1_7:99697321-99697695:- ENST00000465738.1_1_7:99697321-99697695:- MCM7 ncRNA Retained Intron lncRNA GN=MCM7 ncRNA Retained Intron E_20221201_NO30_400nL_HLAc1_2e7_directIP_titration_rep3_Slot2-4_1_3518 20mio-rep3 E_20221201_NO30_400nL_HLAc1_2e7_directIP_titration_rep4_Slot2-4_1_3519.4035.1484.2 2 1 1 10.02 7.26 4.12 2.76 73.0 6 1 1 3600.0 M16m Hydrogen 11 (G) 10 RSSSSmGTSW (F) 10 SAASTSASmSW R S S S|S\m\G\T|S\W 60.0 6 6 2 2 0 b-ion bias m:Oxidized methionine 13.946228 0.823773 551.2313 1085.468 15.9867 -7.5 -1.7000000000000002 9.75 4375.9 nuORFdb_human ENST00000465738.1_1_7:99697321-99697695:- ENST00000465738.1_1_7:99697321-99697695:- MCM7 ncRNA Retained Intron lncRNA GN=MCM7 ncRNA Retained Intron E_20221201_NO30_400nL_HLAc1_2e7_directIP_titration_rep4_Slot2-4_1_3519 40mio-rep1 E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep1_Slot1-04_1_3496.4247.1473.2 2 3 1 11.23 7.94 3.48 3.29 73.3 7 1 1 9350.0 M16m Hydrogen 11 (G) 10 RSSSSmGTSW (F) 10 qSPPAMAGSSW R S S\S/S\m\G\T|S\W 70.0 7 6 2 1 0 b-ion bias m:Oxidized methionine 14.68091 0.830691 551.2329 1085.468 15.9907 -3.8 -0.5999999999999996 9.75 4375.9 nuORFdb_human ENST00000465738.1_1_7:99697321-99697695:- ENST00000465738.1_1_7:99697321-99697695:- MCM7 ncRNA Retained Intron lncRNA GN=MCM7 ncRNA Retained Intron E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep1_Slot1-04_1_3496 40mio-rep2 E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep2_Slot2-5_1_3520.4002.1481.2 2 1 1 9.51 5.84 3.87 3.67 75.6 6 1 1 5910.0 M16m Hydrogen 11 (G) 10 RSSSSmGTSW (F) 10 SAASTSASmSW R S S\S S\m\G\T|S\W 50.0 5 6 1 1 0 b-ion bias m:Oxidized methionine 13.834776 0.825412 551.2316 1085.468 15.9887 -5.7 -0.7999999999999998 9.75 4375.9 nuORFdb_human ENST00000465738.1_1_7:99697321-99697695:- ENST00000465738.1_1_7:99697321-99697695:- MCM7 ncRNA Retained Intron lncRNA GN=MCM7 ncRNA Retained Intron E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep2_Slot2-5_1_3520 40mio-rep4 E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep4_Slot2-5_1_3522.4021.1486.2 2 1 1 14.65 8.97 5.04 5.67 82.4 6 1 1 5490.0 M16m Hydrogen 11 (G) 10 RSSSSmGTSW (F) 10 VYQNSAmSW R S S S\S|m\G|T|S\W 60.0 6 6 3 3 0 b-ion bias m:Oxidized methionine 13.895183 0.822301 551.2325 1085.468 15.9907 -3.8 0.9000000000000004 9.75 4375.9 nuORFdb_human ENST00000465738.1_1_7:99697321-99697695:- ENST00000465738.1_1_7:99697321-99697695:- MCM7 ncRNA Retained Intron lncRNA GN=MCM7 ncRNA Retained Intron E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep4_Slot2-5_1_3522 10mio-rep1 E_20221201_NO30_400nL_HLAc1_1e7_directIP_titration_rep1_Slot2-3_1_3512.11967.1429.2 2 1 1 9.41 6.62 4.33 2.79 59.7 6 1 1 4590.0 Hydrogen 23 (S) 10 RSVAQDGVQW (R) 10 GAWRGWLGGW R S V A\Q|D\G|V|Q\W 60.0 6 6 3 3 0 b-ion bias 41.344532 0.858464 573.2835 1145.57 -0.0091 -7.9 -0.20000000000000018 5.84 5009.5 nuORFdb_human ENST00000429065.2_1_3:10052006-10052764:- ENST00000429065.2_1_3:10052006-10052764:- AC022007.5 lincRNA lncRNA GN=AC022007.5 lincRNA E_20221201_NO30_400nL_HLAc1_1e7_directIP_titration_rep1_Slot2-3_1_3512 10mio-rep3 E_20221201_NO30_400nL_HLAc1_1e7_directIP_titration_rep3_Slot2-3_1_3514.11925.1420.2 2 1 1 9.99 7.02 2.97 2.96 70.4 6 1 1 3340.0 Hydrogen 23 (S) 10 RSVAQDGVQW (R) 10 QLSGVGDRQW R S V\A Q/D\G\V|Q\W 50.0 5 5 2 1 0 b-ion bias 41.200394 0.863992 573.2857 1145.57 -0.0051 -4.4 1.8999999999999995 5.84 5009.5 nuORFdb_human ENST00000429065.2_1_3:10052006-10052764:- ENST00000429065.2_1_3:10052006-10052764:- AC022007.5 lincRNA lncRNA GN=AC022007.5 lincRNA E_20221201_NO30_400nL_HLAc1_1e7_directIP_titration_rep3_Slot2-3_1_3514 40mio-rep1 E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep1_Slot1-04_1_3496.12161.1371.2 2 1 1 9.7 6.26 4.05 3.44 65.6 6 1 1 12700.0 Hydrogen 23 (S) 10 RSVAQDGVQW (R) 10 qASFSPIVQW R S V A\Q\D|G\V|Q\W 60.0 6 6 2 2 0 b-ion bias 42.008621 0.894366 573.2885 1145.57 -0.0011 -0.9 2.3000000000000003 5.84 5009.5 nuORFdb_human ENST00000429065.2_1_3:10052006-10052764:- ENST00000429065.2_1_3:10052006-10052764:- AC022007.5 lincRNA lncRNA GN=AC022007.5 lincRNA E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep1_Slot1-04_1_3496 40mio-rep2 E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep2_Slot2-5_1_3520.11843.1411.2 2 2 1 12.21 8.58 4.23 3.63 80.6 6 1 1 16900.0 Hydrogen 23 (S) 10 RSVAQDGVQW (R) 10 RLWEEVQW R S V\A Q/D|G\V|Q\W 50.0 5 5 3 2 0 unbiased 40.910992 0.869231 573.2882 1145.57 -0.0011 -0.9 4.0 5.84 5009.5 nuORFdb_human ENST00000429065.2_1_3:10052006-10052764:- ENST00000429065.2_1_3:10052006-10052764:- AC022007.5 lincRNA lncRNA GN=AC022007.5 lincRNA E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep2_Slot2-5_1_3520 40mio-rep3 E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep3_Slot2-5_1_3521.5602.1725.3 3 1 1 7.5 5.15 3.41 2.35 67.2 5 1 1 4130.0 Hydrogen 4 (K) 11 RSVGGLAPTPL (V) 11 GVSRGPSPVVL R S V G|G/L A/P|T P\L 27.3 3 3 4 2 0 unbiased 19.355917 0.67097 356.5455 1067.621 -0.0003 -0.3 4.3 9.75 1520.8 nuORFdb_human ENST00000415922.6_1_6:26659853-26659901:- ENST00000415922.6_1_6:26659853-26659901:- ZNF322 5' uORF 5' uORF GN=ZNF322 5' uORF E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep3_Slot2-5_1_3521 20mio-rep2 E_20221201_NO30_400nL_HLAc1_2e7_directIP_titration_rep3_Slot2-4_1_3518.12143.1459.2 2 1 1 8.65 8.65 8.65 60.3 7 1 1 5770.0 Hydrogen 19 (P) 11 RSVPAGVGGCF (-) 11 R S V|P A/G\V|G\G\C\F 54.5 6 6 3 2 0 b-ion bias Carbamidomethylation C:Carbamidomethylation, 41.944096 0.839174 553.7707 1106.541 -0.0064 -5.8 0.5 9.75 2978.4 nuORFdb_human ENST00000537992.5_1_7:20370649-20370736:+ ENST00000537992.5_1_7:20370649-20370736:+ ITGB8 5' uORF 5' uORF GN=ITGB8 5' uORF E_20221201_NO30_400nL_HLAc1_2e7_directIP_titration_rep3_Slot2-4_1_3518 1mio-rep1 E_20221201_NO30_400nL_HLAc1_1e6_directIP_titration_rep1_Slot1-06_1_3498.11325.1432.2 2 1 1 10.19 10.19 10.19 70.3 7 1 1 2150.0 C28c Hydrogen 19 (P) 11 RSVPAGVGGcF (-) 11 R S V|P\A G/V|G\G|c\F 54.5 6 6 4 3 0 unbiased c:Cysteinylation 39.121433 0.856489 584.763 1106.541 61.9776 -4.3 0.6000000000000005 9.75 2978.4 nuORFdb_human ENST00000537992.5_1_7:20370649-20370736:+ ENST00000537992.5_1_7:20370649-20370736:+ ITGB8 5' uORF 5' uORF GN=ITGB8 5' uORF E_20221201_NO30_400nL_HLAc1_1e6_directIP_titration_rep1_Slot1-06_1_3498 10mio-rep1 E_20221201_NO30_400nL_HLAc1_1e7_directIP_titration_rep1_Slot2-3_1_3512.10993.1432.2 2 2 1 12.68 0.11 11.47 12.56 77.5 7 1 1 11200.0 C28c Hydrogen 19 (P) 11 RSVPAGVGGcF (-) 11 GGPVRAATAcF R S V|P A|G/V|G|G|c-F 54.5 6 5 6 5 1 dual b/y bias c:Cysteinylation 37.978565 0.856445 584.7603 1106.541 61.9716 -9.5 -1.7999999999999998 9.75 2978.4 nuORFdb_human ENST00000537992.5_1_7:20370649-20370736:+ ENST00000537992.5_1_7:20370649-20370736:+ ITGB8 5' uORF 5' uORF GN=ITGB8 5' uORF E_20221201_NO30_400nL_HLAc1_1e7_directIP_titration_rep1_Slot2-3_1_3512 10mio-rep2 E_20221201_NO30_400nL_HLAc1_1e7_directIP_titration_rep2_Slot2-3_1_3513.10965.1445.2 2 2 1 12.64 1.74 13.55 10.9 82.3 7 1 1 11400.0 C28c Hydrogen 19 (P) 11 RSVPAGVGGcF (-) 11 qRGTIKcNF R S V|P A|G/V|G|G|c\F 54.5 6 6 6 5 0 dual b/y bias c:Cysteinylation 37.883041 0.848093 584.7614 1106.541 61.9736 -7.7 -1.0 9.75 2978.4 nuORFdb_human ENST00000537992.5_1_7:20370649-20370736:+ ENST00000537992.5_1_7:20370649-20370736:+ ITGB8 5' uORF 5' uORF GN=ITGB8 5' uORF E_20221201_NO30_400nL_HLAc1_1e7_directIP_titration_rep2_Slot2-3_1_3513 10mio-rep3 E_20221201_NO30_400nL_HLAc1_1e7_directIP_titration_rep3_Slot2-3_1_3514.10938.1430.2 2 2 1 10.16 1.31 9.23 8.86 74.2 6 1 1 10000.0 C28c Hydrogen 19 (P) 11 RSVPAGVGGcF (-) 11 qRGTIKcNF R S V/P A|G V|G|G|c\F 36.4 4 5 5 4 0 dual b/y bias c:Cysteinylation 37.790359 0.857352 584.7619 1106.541 61.9756 -6.0 0.2999999999999998 9.75 2978.4 nuORFdb_human ENST00000537992.5_1_7:20370649-20370736:+ ENST00000537992.5_1_7:20370649-20370736:+ ITGB8 5' uORF 5' uORF GN=ITGB8 5' uORF E_20221201_NO30_400nL_HLAc1_1e7_directIP_titration_rep3_Slot2-3_1_3514 20mio-rep1 E_20221201_NO30_400nL_HLAc1_2e7_directIP_titration_rep1_Slot2-4_1_3515.10871.1426.2 2 1 1 14.01 1.67 11.11 12.35 80.2 7 1 1 9580.0 C28c Hydrogen 19 (P) 11 RSVPAGVGGcF (-) 11 GGPVRAATAcF R S/V|P A|G V|G|G|c-F 45.5 5 5 6 5 1 dual b/y bias c:Cysteinylation 37.548481 0.859788 584.7611 1106.541 61.9736 -7.7 -1.5 9.75 2978.4 nuORFdb_human ENST00000537992.5_1_7:20370649-20370736:+ ENST00000537992.5_1_7:20370649-20370736:+ ITGB8 5' uORF 5' uORF GN=ITGB8 5' uORF E_20221201_NO30_400nL_HLAc1_2e7_directIP_titration_rep1_Slot2-4_1_3515 20mio-rep2 E_20221201_NO30_400nL_HLAc1_2e7_directIP_titration_rep3_Slot2-4_1_3518.11010.1425.2 2 1 1 14.21 2.76 11.63 11.45 84.0 7 1 1 6720.0 C28c Hydrogen 19 (P) 11 RSVPAGVGGcF (-) 11 GGPVRAATAcF R S V|P A|G/V|G|G|c\F 54.5 6 6 6 5 0 dual b/y bias c:Cysteinylation 38.029789 0.860736 584.7612 1106.541 61.9736 -7.7 -1.4000000000000004 9.75 2978.4 nuORFdb_human ENST00000537992.5_1_7:20370649-20370736:+ ENST00000537992.5_1_7:20370649-20370736:+ ITGB8 5' uORF 5' uORF GN=ITGB8 5' uORF E_20221201_NO30_400nL_HLAc1_2e7_directIP_titration_rep3_Slot2-4_1_3518 20mio-rep3 E_20221201_NO30_400nL_HLAc1_2e7_directIP_titration_rep4_Slot2-4_1_3519.10915.1421.2 2 1 1 13.15 2.19 13.15 10.96 82.8 6 1 1 13000.0 C28c Hydrogen 19 (P) 11 RSVPAGVGGcF (-) 11 GGPVRAATAcF R S V|P A|G V|G|G|c\F 36.4 4 6 5 5 0 dual b/y bias c:Cysteinylation 37.707458 0.863339 584.7641 1106.541 61.9796 -2.6 3.1999999999999997 9.75 2978.4 nuORFdb_human ENST00000537992.5_1_7:20370649-20370736:+ ENST00000537992.5_1_7:20370649-20370736:+ ITGB8 5' uORF 5' uORF GN=ITGB8 5' uORF E_20221201_NO30_400nL_HLAc1_2e7_directIP_titration_rep4_Slot2-4_1_3519 40mio-rep1 E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep1_Slot1-04_1_3496.11179.1386.2 2 2 1 17.51 4.42 14.43 13.08 90.4 7 1 1 54600.0 C28c Hydrogen 19 (P) 11 RSVPAGVGGcF (-) 11 GGPVRAATAcF R S V|P A|G/V|G|G|c\F 54.5 6 6 6 5 0 dual b/y bias c:Cysteinylation 38.617352 0.885269 584.7639 1106.541 61.9796 -2.6 0.6000000000000001 9.75 2978.4 nuORFdb_human ENST00000537992.5_1_7:20370649-20370736:+ ENST00000537992.5_1_7:20370649-20370736:+ ITGB8 5' uORF 5' uORF GN=ITGB8 5' uORF E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep1_Slot1-04_1_3496 40mio-rep2 E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep2_Slot2-5_1_3520.10888.1407.2 2 2 1 14.17 3.05 12.16 11.12 84.6 7 1 1 66100.0 C28c Hydrogen 19 (P) 11 RSVPAGVGGcF (-) 11 qRGTIKcNF R S V|P A|G/V|G|G|c\F 54.5 6 6 6 5 0 dual b/y bias c:Cysteinylation 37.613781 0.872125 584.7616 1106.541 61.9756 -6.0 -1.0999999999999996 9.75 2978.4 nuORFdb_human ENST00000537992.5_1_7:20370649-20370736:+ ENST00000537992.5_1_7:20370649-20370736:+ ITGB8 5' uORF 5' uORF GN=ITGB8 5' uORF E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep2_Slot2-5_1_3520 40mio-rep3 E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep3_Slot2-5_1_3521.10889.1406.2 2 1 1 17.95 2.65 16.01 15.31 89.6 7 1 1 9750.0 C28c Hydrogen 19 (P) 11 RSVPAGVGGcF (-) 11 qRGTIKcNF R S V|P A|G/V|G|G|c\F 54.5 6 6 6 5 0 dual b/y bias c:Cysteinylation 37.619865 0.87242 584.7633 1106.541 61.9776 -4.3 0.2999999999999998 9.75 2978.4 nuORFdb_human ENST00000537992.5_1_7:20370649-20370736:+ ENST00000537992.5_1_7:20370649-20370736:+ ITGB8 5' uORF 5' uORF GN=ITGB8 5' uORF E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep3_Slot2-5_1_3521 40mio-rep4 E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep4_Slot2-5_1_3522.10893.1406.2 2 1 1 13.41 1.3 13.41 12.11 80.8 6 1 1 13800.0 C28c Hydrogen 19 (P) 11 RSVPAGVGGcF (-) 11 cGGKTKATHF R S V|P A|G V|G|G|c\F 36.4 4 6 5 5 0 dual b/y bias c:Cysteinylation 37.61908 0.8723 584.764 1106.541 61.9796 -2.6 2.1 9.75 2978.4 nuORFdb_human ENST00000537992.5_1_7:20370649-20370736:+ ENST00000537992.5_1_7:20370649-20370736:+ ITGB8 5' uORF 5' uORF GN=ITGB8 5' uORF E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep4_Slot2-5_1_3522 5mio-rep1 E_20221201_NO30_400nL_HLAc1_5e6_directIP_titration_rep1_Slot1-37_1_3502.11247.1426.2 2 2 1 9.93 1.43 9.09 8.49 77.4 6 1 1 10500.0 C28c Hydrogen 19 (P) 11 RSVPAGVGGcF (-) 11 GGPVRAATAcF R S V|P A\G V|G|G|c\F 36.4 4 6 4 4 0 dual b/y bias c:Cysteinylation 38.852703 0.860089 584.7626 1106.541 61.9776 -4.3 2.7 9.75 2978.4 nuORFdb_human ENST00000537992.5_1_7:20370649-20370736:+ ENST00000537992.5_1_7:20370649-20370736:+ ITGB8 5' uORF 5' uORF GN=ITGB8 5' uORF E_20221201_NO30_400nL_HLAc1_5e6_directIP_titration_rep1_Slot1-37_1_3502 5mio-rep2 E_20221201_NO30_400nL_HLAc1_5e6_directIP_titration_rep2_Slot2-2_1_3510.11182.1432.2 2 4 1 10.87 10.87 10.87 69.6 6 1 1 12300.0 C28c Hydrogen 19 (P) 11 RSVPAGVGGcF (-) 11 R S V|P A|G V|G|G\c\F 36.4 4 6 4 4 0 dual b/y bias c:Cysteinylation 38.623405 0.856008 584.7591 1106.541 61.9696 -11.2 -2.5 9.75 2978.4 nuORFdb_human ENST00000537992.5_1_7:20370649-20370736:+ ENST00000537992.5_1_7:20370649-20370736:+ ITGB8 5' uORF 5' uORF GN=ITGB8 5' uORF E_20221201_NO30_400nL_HLAc1_5e6_directIP_titration_rep2_Slot2-2_1_3510 20mio-rep2 E_20221201_NO30_400nL_HLAc1_2e7_directIP_titration_rep3_Slot2-4_1_3518.19903.1446.2 2 1 1 12.56 6.05 7.57 6.51 84.9 5 1 1 7730.0 Hydrogen 71 (Q) 9 RSVPVVMQW (V) 9 RSVAQAGVQW R S V|P V|V|M|Q\W 44.4 4 5 4 4 0 dual b/y bias 68.749809 0.847374 551.2943 1101.587 -0.0066 -6.0 0.2999999999999998 9.75 9579.8 nuORFdb_human ENST00000499346.6_1_5:127418462-127418714:- ENST00000499346.6_1_5:127418462-127418714:- LINC01184 lincRNA lncRNA GN=LINC01184 lincRNA E_20221201_NO30_400nL_HLAc1_2e7_directIP_titration_rep3_Slot2-4_1_3518 40mio-rep1 E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep1_Slot1-04_1_3496.20090.1423.2 2 3 1 16.9 6.59 11.45 10.31 91.9 6 1 1 78000.0 Hydrogen 71 (Q) 9 RSVPVVMQW (V) 9 RSVAQAGVQW R S V|P\V|V|M|Q\W 66.7 6 6 4 4 0 dual b/y bias 69.401344 0.861868 551.2956 1101.587 -0.0026 -2.4 0.8000000000000003 9.75 9579.8 nuORFdb_human ENST00000499346.6_1_5:127418462-127418714:- ENST00000499346.6_1_5:127418462-127418714:- LINC01184 lincRNA lncRNA GN=LINC01184 lincRNA E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep1_Slot1-04_1_3496 10mio-rep1 E_20221201_NO30_400nL_HLAc1_1e7_directIP_titration_rep1_Slot2-3_1_3512.14753.1441.2 2 3 1 14.08 3.61 8.06 10.48 86.3 5 1 1 46400.0 M77m Hydrogen 71 (Q) 9 RSVPVVmQW (V) 9 FQGmKKLSY R S V|P V|V|m|Q\W 44.4 4 5 4 4 0 dual b/y bias m:Oxidized methionine 50.967033 0.850495 559.2895 1101.587 15.9834 -10.3 -2.6000000000000005 9.75 9579.8 nuORFdb_human ENST00000499346.6_1_5:127418462-127418714:- ENST00000499346.6_1_5:127418462-127418714:- LINC01184 lincRNA lncRNA GN=LINC01184 lincRNA E_20221201_NO30_400nL_HLAc1_1e7_directIP_titration_rep1_Slot2-3_1_3512 10mio-rep2 E_20221201_NO30_400nL_HLAc1_1e7_directIP_titration_rep2_Slot2-3_1_3513.14787.1441.2 2 5 1 17.39 5.31 12.33 12.08 92.1 6 1 1 52300.0 M77m Hydrogen 71 (Q) 9 RSVPVVmQW (V) 9 RSVAQSGVQW R S V|P\V|V|m|Q\W 66.7 6 6 4 4 0 dual b/y bias m:Oxidized methionine 51.081802 0.850447 559.2909 1101.587 15.9874 -6.8 -0.09999999999999964 9.75 9579.8 nuORFdb_human ENST00000499346.6_1_5:127418462-127418714:- ENST00000499346.6_1_5:127418462-127418714:- LINC01184 lincRNA lncRNA GN=LINC01184 lincRNA E_20221201_NO30_400nL_HLAc1_1e7_directIP_titration_rep2_Slot2-3_1_3513 10mio-rep3 E_20221201_NO30_400nL_HLAc1_1e7_directIP_titration_rep3_Slot2-3_1_3514.14725.1444.2 2 2 1 15.65 4.31 9.67 11.34 91.1 5 1 1 33200.0 M77m Hydrogen 71 (Q) 9 RSVPVVmQW (V) 9 RSVAQSGVQW R S V|P V|V|m|Q\W 44.4 4 5 4 4 0 dual b/y bias m:Oxidized methionine 50.870029 0.848529 559.2913 1101.587 15.9874 -6.8 -0.5 9.75 9579.8 nuORFdb_human ENST00000499346.6_1_5:127418462-127418714:- ENST00000499346.6_1_5:127418462-127418714:- LINC01184 lincRNA lncRNA GN=LINC01184 lincRNA E_20221201_NO30_400nL_HLAc1_1e7_directIP_titration_rep3_Slot2-3_1_3514 20mio-rep1 E_20221201_NO30_400nL_HLAc1_2e7_directIP_titration_rep1_Slot2-4_1_3515.14644.1429.2 2 8 1 16.11 3.49 11.25 12.62 87.4 6 1 1 129000.0 M77m Hydrogen 71 (Q) 9 RSVPVVmQW (V) 9 FQGmKKLSY R S V|P|V|V|m|Q\W 66.7 6 6 5 5 0 dual b/y bias m:Oxidized methionine 50.577164 0.858048 559.2914 1101.587 15.9874 -6.8 -0.5999999999999996 9.75 9579.8 nuORFdb_human ENST00000499346.6_1_5:127418462-127418714:- ENST00000499346.6_1_5:127418462-127418714:- LINC01184 lincRNA lncRNA GN=LINC01184 lincRNA E_20221201_NO30_400nL_HLAc1_2e7_directIP_titration_rep1_Slot2-4_1_3515 20mio-rep2 E_20221201_NO30_400nL_HLAc1_2e7_directIP_titration_rep3_Slot2-4_1_3518.14864.1417.2 2 3 1 15.73 3.52 11.59 12.21 86.0 5 1 1 74900.0 M77m Hydrogen 71 (Q) 9 RSVPVVmQW (V) 9 RSVAQSGVQW R S V|P V|V|m|Q\W 44.4 4 5 4 4 0 dual b/y bias m:Oxidized methionine 51.341366 0.865874 559.291 1101.587 15.9874 -6.8 -0.5 9.75 9579.8 nuORFdb_human ENST00000499346.6_1_5:127418462-127418714:- ENST00000499346.6_1_5:127418462-127418714:- LINC01184 lincRNA lncRNA GN=LINC01184 lincRNA E_20221201_NO30_400nL_HLAc1_2e7_directIP_titration_rep3_Slot2-4_1_3518 20mio-rep3 E_20221201_NO30_400nL_HLAc1_2e7_directIP_titration_rep4_Slot2-4_1_3519.14809.1434.2 2 3 1 15.28 3.63 9.8 11.65 81.3 5 1 1 60000.0 M77m Hydrogen 71 (Q) 9 RSVPVVmQW (V) 9 RSVAQSGVQW R S V|P V|V|m|Q\W 44.4 4 5 4 4 0 dual b/y bias m:Oxidized methionine 51.150742 0.855315 559.2906 1101.587 15.9874 -6.8 -1.0 9.75 9579.8 nuORFdb_human ENST00000499346.6_1_5:127418462-127418714:- ENST00000499346.6_1_5:127418462-127418714:- LINC01184 lincRNA lncRNA GN=LINC01184 lincRNA E_20221201_NO30_400nL_HLAc1_2e7_directIP_titration_rep4_Slot2-4_1_3519 40mio-rep1 E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep1_Slot1-04_1_3496.14930.1417.2 2 6 1 18.08 4.81 12.01 13.27 90.5 5 1 1 245000.0 M77m Hydrogen 71 (Q) 9 RSVPVVmQW (V) 9 VPAGmTVIQW R S V|P V|V|m|Q\W 44.4 4 5 4 4 0 dual b/y bias m:Oxidized methionine 51.572712 0.86594 559.2939 1101.587 15.9934 -1.4 1.8000000000000003 9.75 9579.8 nuORFdb_human ENST00000499346.6_1_5:127418462-127418714:- ENST00000499346.6_1_5:127418462-127418714:- LINC01184 lincRNA lncRNA GN=LINC01184 lincRNA E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep1_Slot1-04_1_3496 40mio-rep2 E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep2_Slot2-5_1_3520.14570.1422.2 2 5 1 17.53 4.14 11.65 13.39 90.3 6 1 1 108000.0 M77m Hydrogen 71 (Q) 9 RSVPVVmQW (V) 9 RSVAQSGVQW R S V|P\V|V|m|Q\W 66.7 6 6 4 4 0 dual b/y bias m:Oxidized methionine 50.331039 0.862617 559.2927 1101.587 15.9914 -3.2 1.7000000000000002 9.75 9579.8 nuORFdb_human ENST00000499346.6_1_5:127418462-127418714:- ENST00000499346.6_1_5:127418462-127418714:- LINC01184 lincRNA lncRNA GN=LINC01184 lincRNA E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep2_Slot2-5_1_3520 40mio-rep3 E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep3_Slot2-5_1_3521.14579.1431.2 2 7 1 17.84 3.82 12.13 14.02 90.8 6 1 1 123000.0 M77m Hydrogen 71 (Q) 9 RSVPVVmQW (V) 9 RSVAQSGVQW R S V|P|V|V|m|Q\W 66.7 6 6 5 5 0 dual b/y bias m:Oxidized methionine 50.364292 0.856618 559.2926 1101.587 15.9914 -3.2 1.3999999999999995 9.75 9579.8 nuORFdb_human ENST00000499346.6_1_5:127418462-127418714:- ENST00000499346.6_1_5:127418462-127418714:- LINC01184 lincRNA lncRNA GN=LINC01184 lincRNA E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep3_Slot2-5_1_3521 40mio-rep4 E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep4_Slot2-5_1_3522.14666.1427.2 2 7 1 18.31 4.66 11.38 13.65 90.2 6 1 1 174000.0 M77m Hydrogen 71 (Q) 9 RSVPVVmQW (V) 9 FQGmKKLSY R S V|P\V|V|m|Q\W 66.7 6 6 4 4 0 dual b/y bias m:Oxidized methionine 50.650631 0.859575 559.2921 1101.587 15.9894 -5.0 -0.2999999999999998 9.75 9579.8 nuORFdb_human ENST00000499346.6_1_5:127418462-127418714:- ENST00000499346.6_1_5:127418462-127418714:- LINC01184 lincRNA lncRNA GN=LINC01184 lincRNA E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep4_Slot2-5_1_3522 5mio-rep1 E_20221201_NO30_400nL_HLAc1_5e6_directIP_titration_rep1_Slot1-37_1_3502.14974.1434.2 2 3 1 13.64 3.72 9.96 9.92 81.0 5 1 1 29600.0 M77m Hydrogen 71 (Q) 9 RSVPVVmQW (V) 9 RSVAQSGVQW R S V|P V|V|m|Q\W 44.4 4 5 4 4 0 dual b/y bias m:Oxidized methionine 51.723557 0.855269 559.2902 1101.587 15.9854 -8.5 -1.5 9.75 9579.8 nuORFdb_human ENST00000499346.6_1_5:127418462-127418714:- ENST00000499346.6_1_5:127418462-127418714:- LINC01184 lincRNA lncRNA GN=LINC01184 lincRNA E_20221201_NO30_400nL_HLAc1_5e6_directIP_titration_rep1_Slot1-37_1_3502 40mio-rep2 E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep2_Slot2-5_1_3520.14799.1431.2 2 1 1 13.74 10.82 5.46 2.93 80.6 6 1 1 9240.0 Hydrogen 5 (S) 10 RSVVQAGVQW (R) 10 GLTVAQAGVQW R S V V|Q\A|G\V|Q\W 60.0 6 6 3 3 0 b-ion bias 51.121628 0.856961 565.3065 1129.611 -0.0045 -4.0 0.9000000000000004 9.75 5129.8 nuORFdb_human ENST00000496525.1_1_13:20244500-20245123:+ ENST00000496525.1_1_13:20244500-20245123:+|ENST00000399120.5_1_21:38320506-38334458:- MPHOSPH8 ncRNA Processed Transcript lncRNA GN=MPHOSPH8 ncRNA Processed Transcript E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep2_Slot2-5_1_3520 40mio-rep4 E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep4_Slot2-5_1_3522.14829.1433.2 2 1 1 11.4 10.18 3.2 1.21 76.6 6 1 1 10100.0 Hydrogen 5 (S) 10 RSVVQAGVQW (R) 10 GLTVAQAGVQW R S V V|Q\A|G\V|Q\W 60.0 6 6 3 3 0 b-ion bias 51.213264 0.855346 565.3049 1129.611 -0.0085 -7.6 -2.8999999999999995 9.75 5129.8 nuORFdb_human ENST00000496525.1_1_13:20244500-20245123:+ ENST00000496525.1_1_13:20244500-20245123:+|ENST00000399120.5_1_21:38320506-38334458:- MPHOSPH8 ncRNA Processed Transcript lncRNA GN=MPHOSPH8 ncRNA Processed Transcript E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep4_Slot2-5_1_3522 40mio-rep1 E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep1_Slot1-04_1_3496.23690.1266.2 2 4 1 15.95 5.3 10.65 10.65 87.8 7 1 1 28700.0 Hydrogen 63 (M) 11 RSWLIATSASW (V) 11 LKDYALPNASW R S W L|I\A|T\S|A|S\W 63.6 7 7 4 4 0 dual b/y bias 81.838463 0.959801 639.332 1277.664 -0.007 -5.5 -2.3 9.75 10238.0 nuORFdb_human ENST00000444363.5_1_7:87488057-87505510:- ENST00000444363.5_1_7:87488057-87505510:- SLC25A40 5' uORF 5' uORF GN=SLC25A40 5' uORF E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep1_Slot1-04_1_3496 40mio-rep2 E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep2_Slot2-5_1_3520.21328.1320.2 2 1 1 10.28 5.71 7.57 4.57 64.2 7 1 1 5140.0 Hydrogen 26 (A) 11 RSWLTAASTSW (A) 11 RSWLTATSASW R S W L\T|A|A|S\T|S\W 63.6 7 7 4 4 0 dual b/y bias 73.679779 0.926387 633.3117 1265.627 -0.0106 -8.4 -3.5 9.75 16357.4 nuORFdb_human ENST00000511526.5_1_3:128616515-128622914:+ ENST00000511526.5_1_3:128616515-128622914:+ ACAD9 3' Overlap dORF 3' Overlap dORF GN=ACAD9 3' Overlap dORF E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep2_Slot2-5_1_3520 40mio-rep1 E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep1_Slot1-04_1_3496.8112.1573.3 3 1 1 10.5 5.2 4.82 5.3 73.8 6 2 2 14200.0 C79c Hydrogen 75 (G) 9 RSYPcQRLL (-) 9 DDDKHDQRLL R S Y/P/c|Q/R\L\L 66.7 6 3 4 1 0 unbiased c:Cysteinylation 28.027348 0.767728 418.8723 1192.626 61.9759 -5.4 -2.2 10.84 9404.6 nuORFdb_human ENST00000268797.11_1_16:67719651-67753260:- ENST00000268797.11_1_16:67719651-67753260:- GFOD2 5' uORF 5' uORF GN=GFOD2 5' uORF E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep1_Slot1-04_1_3496 40mio-rep2 E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep2_Slot2-5_1_3520.7845.1597.3 3 2 1 7.59 3.02 3.93 4.57 60.4 6 2 2 29800.0 C79c Hydrogen 75 (G) 9 RSYPcQRLL (-) 9 DDDKHDQRLL R S Y/P/c|Q|R\L\L 66.7 6 4 4 2 0 unbiased c:Cysteinylation 27.105385 0.752434 418.8714 1192.626 61.9729 -7.8 -2.8999999999999995 10.84 9404.6 nuORFdb_human ENST00000268797.11_1_16:67719651-67753260:- ENST00000268797.11_1_16:67719651-67753260:- GFOD2 5' uORF 5' uORF GN=GFOD2 5' uORF E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep2_Slot2-5_1_3520 40mio-rep3 E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep3_Slot2-5_1_3521.7843.1594.3 3 4 1 10.15 4.06 5.49 6.09 68.7 6 2 2 30500.0 C79c Hydrogen 75 (G) 9 RSYPcQRLL (-) 9 DDDKHDQRLL R S Y/P|c|Q|R\L\L 66.7 6 5 4 3 0 dual b/y bias c:Cysteinylation 27.095451 0.754164 418.8721 1192.626 61.9759 -5.4 -0.8000000000000007 10.84 9404.6 nuORFdb_human ENST00000268797.11_1_16:67719651-67753260:- ENST00000268797.11_1_16:67719651-67753260:- GFOD2 5' uORF 5' uORF GN=GFOD2 5' uORF E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep3_Slot2-5_1_3521 1mio-rep1 E_20221201_NO30_400nL_HLAc1_1e6_directIP_titration_rep1_Slot1-06_1_3498.17964.1415.2 2 1 1 8.7 7.08 2.83 1.62 74.3 5 1 1 2060.0 Hydrogen 40 (V) 11 RTAAGMEGLSW (L) 11 nSVCVRGISW R T A A\G M E|G\L|S\W 36.4 4 5 2 2 0 b-ion bias 62.041851 0.866727 589.7802 1178.562 -0.0095 -8.1 -3.1999999999999993 6.0 5582.4 nuORFdb_human ENST00000378119.8_1_6:24720562-24720727:- ENST00000378119.8_1_6:24720562-24720727:- C6orf62 5' uORF 5' uORF GN=C6orf62 5' uORF E_20221201_NO30_400nL_HLAc1_1e6_directIP_titration_rep1_Slot1-06_1_3498 10mio-rep2 E_20221201_NO30_400nL_HLAc1_1e7_directIP_titration_rep2_Slot2-3_1_3513.17689.1393.2 2 1 1 8.15 6.59 2.12 1.57 68.6 5 1 1 4340.0 Hydrogen 40 (V) 11 RTAAGMEGLSW (L) 11 SWIMQALGSW R T A A\G M E\G\L|S\W 36.4 4 5 1 1 0 b-ion bias 61.103321 0.880691 589.7814 1178.562 -0.0075 -6.4 0.2999999999999998 6.0 5582.4 nuORFdb_human ENST00000378119.8_1_6:24720562-24720727:- ENST00000378119.8_1_6:24720562-24720727:- C6orf62 5' uORF 5' uORF GN=C6orf62 5' uORF E_20221201_NO30_400nL_HLAc1_1e7_directIP_titration_rep2_Slot2-3_1_3513 20mio-rep1 E_20221201_NO30_400nL_HLAc1_2e7_directIP_titration_rep1_Slot2-4_1_3515.17647.1382.2 2 2 1 11.75 8.6 4.44 3.15 80.7 6 1 1 20400.0 Hydrogen 40 (V) 11 RTAAGMEGLSW (L) 11 nSVCVRGISW R T A A|G\M E|G\L|S\W 45.5 5 6 3 3 0 b-ion bias 60.946411 0.887634 589.7808 1178.562 -0.0075 -6.4 -0.20000000000000018 6.0 5582.4 nuORFdb_human ENST00000378119.8_1_6:24720562-24720727:- ENST00000378119.8_1_6:24720562-24720727:- C6orf62 5' uORF 5' uORF GN=C6orf62 5' uORF E_20221201_NO30_400nL_HLAc1_2e7_directIP_titration_rep1_Slot2-4_1_3515 20mio-rep2 E_20221201_NO30_400nL_HLAc1_2e7_directIP_titration_rep3_Slot2-4_1_3518.17716.1385.2 2 1 1 11.65 8.49 4.5 3.16 83.1 6 1 1 5940.0 Hydrogen 40 (V) 11 RTAAGMEGLSW (L) 11 nSVCVRGISW R T A A|G\M E|G\L|S\W 45.5 5 6 3 3 0 b-ion bias 61.193531 0.885847 589.7808 1178.562 -0.0075 -6.4 -0.10000000000000053 6.0 5582.4 nuORFdb_human ENST00000378119.8_1_6:24720562-24720727:- ENST00000378119.8_1_6:24720562-24720727:- C6orf62 5' uORF 5' uORF GN=C6orf62 5' uORF E_20221201_NO30_400nL_HLAc1_2e7_directIP_titration_rep3_Slot2-4_1_3518 20mio-rep3 E_20221201_NO30_400nL_HLAc1_2e7_directIP_titration_rep4_Slot2-4_1_3519.17682.1377.2 2 2 1 12.67 11.08 4.41 1.59 87.3 6 1 1 14200.0 Hydrogen 40 (V) 11 RTAAGMEGLSW (L) 11 nSVCVRGISW R T A A\G\M E|G\L|S\W 45.5 5 6 2 2 0 b-ion bias 61.072136 0.890814 589.7805 1178.562 -0.0095 -8.1 -2.3 6.0 5582.4 nuORFdb_human ENST00000378119.8_1_6:24720562-24720727:- ENST00000378119.8_1_6:24720562-24720727:- C6orf62 5' uORF 5' uORF GN=C6orf62 5' uORF E_20221201_NO30_400nL_HLAc1_2e7_directIP_titration_rep4_Slot2-4_1_3519 40mio-rep1 E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep1_Slot1-04_1_3496.17844.1366.2 2 4 1 19.36 14.79 7.16 4.57 96.6 7 1 1 70300.0 Hydrogen 40 (V) 11 RTAAGMEGLSW (L) 11 nSVCVRGISW R T A\A|G\M E|G|L|S\W 54.5 6 7 4 4 0 dual b/y bias 61.6399 0.897778 589.7838 1178.562 -0.0015 -1.3 1.9000000000000001 6.0 5582.4 nuORFdb_human ENST00000378119.8_1_6:24720562-24720727:- ENST00000378119.8_1_6:24720562-24720727:- C6orf62 5' uORF 5' uORF GN=C6orf62 5' uORF E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep1_Slot1-04_1_3496 40mio-rep2 E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep2_Slot2-5_1_3520.17527.1370.2 2 1 1 16.2 12.08 6.16 4.12 92.4 8 1 1 10800.0 Hydrogen 40 (V) 11 RTAAGMEGLSW (L) 11 nSVCVRGISW R T A/A\G\M\E|G\L|S\W 72.7 8 7 3 2 0 b-ion bias 60.546219 0.895258 589.7824 1178.562 -0.0055 -4.7 0.20000000000000018 6.0 5582.4 nuORFdb_human ENST00000378119.8_1_6:24720562-24720727:- ENST00000378119.8_1_6:24720562-24720727:- C6orf62 5' uORF 5' uORF GN=C6orf62 5' uORF E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep2_Slot2-5_1_3520 40mio-rep3 E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep3_Slot2-5_1_3521.17578.1365.2 2 2 1 11.17 9.08 3.87 2.09 81.0 5 1 1 20500.0 Hydrogen 40 (V) 11 RTAAGMEGLSW (L) 11 nSVCVRGISW R T A A|G M E|G\L|S\W 36.4 4 5 3 3 0 unbiased 60.723545 0.898297 589.7817 1178.562 -0.0055 -4.7 -0.10000000000000053 6.0 5582.4 nuORFdb_human ENST00000378119.8_1_6:24720562-24720727:- ENST00000378119.8_1_6:24720562-24720727:- C6orf62 5' uORF 5' uORF GN=C6orf62 5' uORF E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep3_Slot2-5_1_3521 40mio-rep4 E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep4_Slot2-5_1_3522.17551.1362.2 2 4 1 12.67 9.53 4.97 3.14 82.7 6 1 1 33700.0 Hydrogen 40 (V) 11 RTAAGMEGLSW (L) 11 nSVCVRGISW R T A A|G\M E|G\L|S\W 45.5 5 6 3 3 0 b-ion bias 60.612278 0.900392 589.781 1178.562 -0.0075 -6.4 -1.7000000000000002 6.0 5582.4 nuORFdb_human ENST00000378119.8_1_6:24720562-24720727:- ENST00000378119.8_1_6:24720562-24720727:- C6orf62 5' uORF 5' uORF GN=C6orf62 5' uORF E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep4_Slot2-5_1_3522 5mio-rep1 E_20221201_NO30_400nL_HLAc1_5e6_directIP_titration_rep1_Slot1-37_1_3502.17945.1406.2 2 1 1 15.39 11.18 7.35 4.21 85.2 8 1 1 5750.0 Hydrogen 40 (V) 11 RTAAGMEGLSW (L) 11 nSVCVRGISW R T A/A\G/M\E|G\L|S\W 72.7 8 6 4 2 0 unbiased 61.983475 0.872381 589.7816 1178.562 -0.0055 -4.7 2.3 6.0 5582.4 nuORFdb_human ENST00000378119.8_1_6:24720562-24720727:- ENST00000378119.8_1_6:24720562-24720727:- C6orf62 5' uORF 5' uORF GN=C6orf62 5' uORF E_20221201_NO30_400nL_HLAc1_5e6_directIP_titration_rep1_Slot1-37_1_3502 10mio-rep1 E_20221201_NO30_400nL_HLAc1_1e7_directIP_titration_rep1_Slot2-3_1_3512.12971.1413.2 2 1 1 7.85 5.65 1.2 2.19 57.2 5 1 1 25700.0 M45m Hydrogen 40 (V) 11 RTAAGmEGLSW (L) 11 mRnKQnISW R T A\A\G m E G\L|S\W 36.4 4 5 1 1 0 b-ion bias m:Oxidized methionine 44.812893 0.868515 597.7787 1178.562 15.9885 -5.4 2.3 6.0 5582.4 nuORFdb_human ENST00000378119.8_1_6:24720562-24720727:- ENST00000378119.8_1_6:24720562-24720727:- C6orf62 5' uORF 5' uORF GN=C6orf62 5' uORF E_20221201_NO30_400nL_HLAc1_1e7_directIP_titration_rep1_Slot2-3_1_3512 10mio-rep3 E_20221201_NO30_400nL_HLAc1_1e7_directIP_titration_rep3_Slot2-3_1_3514.12894.1453.2 2 2 1 13.6 8.85 4.29 4.75 80.4 7 1 1 59500.0 M45m Hydrogen 40 (V) 11 RTAAGmEGLSW (L) 11 SWImQALGSW R T/A A\G m\E|G|L|S\W 45.5 5 6 4 3 0 unbiased m:Oxidized methionine 44.548012 0.843231 597.779 1178.562 15.9885 -5.4 0.8999999999999995 6.0 5582.4 nuORFdb_human ENST00000378119.8_1_6:24720562-24720727:- ENST00000378119.8_1_6:24720562-24720727:- C6orf62 5' uORF 5' uORF GN=C6orf62 5' uORF E_20221201_NO30_400nL_HLAc1_1e7_directIP_titration_rep3_Slot2-3_1_3514 20mio-rep1 E_20221201_NO30_400nL_HLAc1_2e7_directIP_titration_rep1_Slot2-4_1_3515.12861.1404.2 2 1 1 15.51 9.62 5.27 5.89 75.4 8 1 1 95400.0 M45m Hydrogen 40 (V) 11 RTAAGmEGLSW (L) 11 mRnKQnISW R T A\A|G\m\E\G|L|S\W 72.7 8 8 3 3 0 b-ion bias m:Oxidized methionine 44.421635 0.87384 597.7798 1178.562 15.9905 -3.7 2.5 6.0 5582.4 nuORFdb_human ENST00000378119.8_1_6:24720562-24720727:- ENST00000378119.8_1_6:24720562-24720727:- C6orf62 5' uORF 5' uORF GN=C6orf62 5' uORF E_20221201_NO30_400nL_HLAc1_2e7_directIP_titration_rep1_Slot2-4_1_3515 20mio-rep2 E_20221201_NO30_400nL_HLAc1_2e7_directIP_titration_rep3_Slot2-4_1_3518.12971.1403.2 2 2 1 17.14 12.66 5.45 4.48 87.7 7 1 1 88100.0 M45m Hydrogen 40 (V) 11 RTAAGmEGLSW (L) 11 SWImQALGSW R T A\A/G m\E|G|L|S\W 54.5 6 6 4 3 0 unbiased m:Oxidized methionine 44.802578 0.874513 597.7795 1178.562 15.9885 -5.4 0.8999999999999995 6.0 5582.4 nuORFdb_human ENST00000378119.8_1_6:24720562-24720727:- ENST00000378119.8_1_6:24720562-24720727:- C6orf62 5' uORF 5' uORF GN=C6orf62 5' uORF E_20221201_NO30_400nL_HLAc1_2e7_directIP_titration_rep3_Slot2-4_1_3518 20mio-rep3 E_20221201_NO30_400nL_HLAc1_2e7_directIP_titration_rep4_Slot2-4_1_3519.12883.1405.2 2 1 1 15.4 10.04 6.1 5.36 74.7 7 1 1 81500.0 M45m Hydrogen 40 (V) 11 RTAAGmEGLSW (L) 11 SWImQALGSW R T A A|G\m\E|G\L|S\W 63.6 7 7 3 3 0 b-ion bias m:Oxidized methionine 44.502052 0.872968 597.7785 1178.562 15.9885 -5.4 0.39999999999999947 6.0 5582.4 nuORFdb_human ENST00000378119.8_1_6:24720562-24720727:- ENST00000378119.8_1_6:24720562-24720727:- C6orf62 5' uORF 5' uORF GN=C6orf62 5' uORF E_20221201_NO30_400nL_HLAc1_2e7_directIP_titration_rep4_Slot2-4_1_3519 40mio-rep1 E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep1_Slot1-04_1_3496.13119.1363.2 2 1 1 11.99 8.13 4.47 3.86 58.4 6 1 1 11200.0 M45m Hydrogen 40 (V) 11 RTAAGmEGLSW (L) 11 mRnKQnISW R T A A|G m\E\G|L|S\W 45.5 5 6 3 3 0 b-ion bias m:Oxidized methionine 45.317646 0.89929 597.7806 1178.562 15.9925 -2.1 1.1 6.0 5582.4 nuORFdb_human ENST00000378119.8_1_6:24720562-24720727:- ENST00000378119.8_1_6:24720562-24720727:- C6orf62 5' uORF 5' uORF GN=C6orf62 5' uORF E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep1_Slot1-04_1_3496 40mio-rep2 E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep2_Slot2-5_1_3520.12850.1390.2 2 1 1 14.16 10.51 4.7 3.65 75.2 6 1 1 269000.0 M45m Hydrogen 40 (V) 11 RTAAGmEGLSW (L) 11 SWImQALGSW R T A A|G m\E|G\L|S\W 45.5 5 6 3 3 0 b-ion bias m:Oxidized methionine 44.390369 0.882396 597.782 1178.562 15.9945 -0.4 4.5 6.0 5582.4 nuORFdb_human ENST00000378119.8_1_6:24720562-24720727:- ENST00000378119.8_1_6:24720562-24720727:- C6orf62 5' uORF 5' uORF GN=C6orf62 5' uORF E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep2_Slot2-5_1_3520 40mio-rep3 E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep3_Slot2-5_1_3521.12850.1398.2 2 1 1 16.46 10.63 6.66 5.82 74.8 7 1 1 168000.0 M45m Hydrogen 40 (V) 11 RTAAGmEGLSW (L) 11 SWImQALGSW R T A A|G\m\E|G|L|S\W 63.6 7 7 4 4 0 dual b/y bias m:Oxidized methionine 44.39473 0.877752 597.7807 1178.562 15.9925 -2.1 2.4999999999999996 6.0 5582.4 nuORFdb_human ENST00000378119.8_1_6:24720562-24720727:- ENST00000378119.8_1_6:24720562-24720727:- C6orf62 5' uORF 5' uORF GN=C6orf62 5' uORF E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep3_Slot2-5_1_3521 40mio-rep4 E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep4_Slot2-5_1_3522.12839.1396.2 2 1 1 14.68 11.06 4.43 3.63 70.6 6 1 1 157000.0 M45m Hydrogen 40 (V) 11 RTAAGmEGLSW (L) 11 SWImQALGSW R T A A\G m\E|G|L|S\W 45.5 5 6 3 3 0 b-ion bias m:Oxidized methionine 44.337196 0.879017 597.7816 1178.562 15.9945 -0.4 4.3 6.0 5582.4 nuORFdb_human ENST00000378119.8_1_6:24720562-24720727:- ENST00000378119.8_1_6:24720562-24720727:- C6orf62 5' uORF 5' uORF GN=C6orf62 5' uORF E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep4_Slot2-5_1_3522 5mio-rep3 E_20221201_NO30_400nL_HLAc1_5e6_directIP_titration_rep3_Slot2-2_1_3511.13148.1414.2 2 1 1 11.59 6.82 4.91 4.77 70.7 7 1 1 12800.0 M45m Hydrogen 40 (V) 11 RTAAGmEGLSW (L) 11 mRnKQnISW R T A\A\G m\E\G\L|S\W 54.5 6 7 1 1 0 b-ion bias m:Oxidized methionine 45.419323 0.867821 597.7773 1178.562 15.9845 -8.8 -0.6000000000000014 6.0 5582.4 nuORFdb_human ENST00000378119.8_1_6:24720562-24720727:- ENST00000378119.8_1_6:24720562-24720727:- C6orf62 5' uORF 5' uORF GN=C6orf62 5' uORF E_20221201_NO30_400nL_HLAc1_5e6_directIP_titration_rep3_Slot2-2_1_3511 40mio-rep2 E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep2_Slot2-5_1_3520.16284.1509.2 2 1 1 8.33 5.6 3.37 2.73 70.8 6 1 1 5860.0 Hydrogen 44 (Q) 9 RTALGGMSW (M) 9 TRAGFPGSW R T A/L\G\G\M|S\W 66.7 6 5 2 1 0 b-ion bias 56.250362 0.807886 489.7428 978.483 -0.0038 -3.9 1.0000000000000004 9.75 6807.9 nuORFdb_human ENST00000417626.6_1_3:50327849-50329668:- ENST00000417626.6_1_3:50327849-50329668:- IFRD2 Out-of-Frame Out-of-Frame GN=IFRD2 Out-of-Frame E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep2_Slot2-5_1_3520 10mio-rep3 E_20221201_NO30_400nL_HLAc1_1e7_directIP_titration_rep3_Slot2-3_1_3514.10614.1524.2 2 2 1 12.59 6.12 5.83 6.46 69.3 6 1 1 7920.0 M50m Hydrogen 44 (Q) 9 RTALGGmSW (M) 9 KEmLANSW R T/A/L|G G|m|S\W 55.6 5 4 5 3 0 dual b/y bias m:Oxidized methionine 36.672066 0.798481 497.7386 978.483 15.9882 -6.8 -0.5 9.75 6807.9 nuORFdb_human ENST00000417626.6_1_3:50327849-50329668:- ENST00000417626.6_1_3:50327849-50329668:- IFRD2 Out-of-Frame Out-of-Frame GN=IFRD2 Out-of-Frame E_20221201_NO30_400nL_HLAc1_1e7_directIP_titration_rep3_Slot2-3_1_3514 20mio-rep1 E_20221201_NO30_400nL_HLAc1_2e7_directIP_titration_rep1_Slot2-4_1_3515.10545.1517.2 2 2 1 13.65 7.3 6.25 6.36 82.4 6 1 1 14500.0 M50m Hydrogen 44 (Q) 9 RTALGGmSW (M) 9 GQLLSGmSW R T/A|L|G G|m|S\W 55.6 5 5 5 4 0 dual b/y bias m:Oxidized methionine 36.422909 0.802644 497.7396 978.483 15.9902 -4.8 1.4000000000000004 9.75 6807.9 nuORFdb_human ENST00000417626.6_1_3:50327849-50329668:- ENST00000417626.6_1_3:50327849-50329668:- IFRD2 Out-of-Frame Out-of-Frame GN=IFRD2 Out-of-Frame E_20221201_NO30_400nL_HLAc1_2e7_directIP_titration_rep1_Slot2-4_1_3515 20mio-rep3 E_20221201_NO30_400nL_HLAc1_2e7_directIP_titration_rep4_Slot2-4_1_3519.10690.1515.2 2 3 1 13.56 7.83 4.68 5.73 80.9 6 1 1 15800.0 M50m Hydrogen 44 (Q) 9 RTALGGmSW (M) 9 GQLLSGmSW R T/A|L|G G\m|S\W 55.6 5 5 4 3 0 dual b/y bias m:Oxidized methionine 36.930187 0.803925 497.7392 978.483 15.9882 -6.8 -1.0 9.75 6807.9 nuORFdb_human ENST00000417626.6_1_3:50327849-50329668:- ENST00000417626.6_1_3:50327849-50329668:- IFRD2 Out-of-Frame Out-of-Frame GN=IFRD2 Out-of-Frame E_20221201_NO30_400nL_HLAc1_2e7_directIP_titration_rep4_Slot2-4_1_3519 1mio-rep1 E_20221201_NO30_400nL_HLAc1_1e6_directIP_titration_rep1_Slot1-06_1_3498.18333.1394.2 2 2 1 10.41 5.84 3.07 4.57 83.6 6 1 1 6050.0 Hydrogen 16 (E) 9 RTDQFYVVY (E) 9 LPAGWEVnVY R T D\Q\F\Y\V|V\Y 66.7 6 6 1 1 0 b-ion bias 63.3162 0.880214 595.7925 1190.584 -0.0053 -4.5 0.40000000000000036 5.83 4102.6 nuORFdb_human ENST00000537473.2_1_12:57065549-57066549:- ENST00000537473.2_1_12:57065549-57066549:- PTGES3 ncRNA Processed Transcript lncRNA GN=PTGES3 ncRNA Processed Transcript E_20221201_NO30_400nL_HLAc1_1e6_directIP_titration_rep1_Slot1-06_1_3498 1mio-rep2 E_20221201_NO30_400nL_HLAc1_1e6_directIP_titration_rep3_Slot2-1_1_3508.18283.1383.2 2 1 1 11.01 7.12 2.99 3.89 87.8 5 1 1 1450.0 Hydrogen 16 (E) 9 RTDQFYVVY (E) 9 FAWFTSAVVY R T D Q\F\Y|V|V\Y 55.6 5 5 2 2 0 b-ion bias 63.145981 0.886896 595.7893 1190.584 -0.0133 -11.2 -2.8999999999999986 5.83 4102.6 nuORFdb_human ENST00000537473.2_1_12:57065549-57066549:- ENST00000537473.2_1_12:57065549-57066549:- PTGES3 ncRNA Processed Transcript lncRNA GN=PTGES3 ncRNA Processed Transcript E_20221201_NO30_400nL_HLAc1_1e6_directIP_titration_rep3_Slot2-1_1_3508 1mio-rep3 E_20221201_NO30_400nL_HLAc1_1e6_directIP_titration_rep4_Slot2-1_1_3509.18206.1379.2 2 1 1 10.18 5.23 2.87 4.95 83.5 5 1 1 1360.0 Hydrogen 16 (E) 9 RTDQFYVVY (E) 9 FAWFTSAVVY R T D\Q F\Y\V|V\Y 44.4 4 5 1 1 0 b-ion bias 62.87196 0.889545 595.7916 1190.584 -0.0073 -6.2 1.7999999999999998 5.83 4102.6 nuORFdb_human ENST00000537473.2_1_12:57065549-57066549:- ENST00000537473.2_1_12:57065549-57066549:- PTGES3 ncRNA Processed Transcript lncRNA GN=PTGES3 ncRNA Processed Transcript E_20221201_NO30_400nL_HLAc1_1e6_directIP_titration_rep4_Slot2-1_1_3509 10mio-rep1 E_20221201_NO30_400nL_HLAc1_1e7_directIP_titration_rep1_Slot2-3_1_3512.18075.1376.2 2 2 1 12.39 8.92 2.67 3.47 92.5 5 1 1 42700.0 Hydrogen 16 (E) 9 RTDQFYVVY (E) 9 FAWFTSAVVY R T D Q\F|Y|V|V\Y 55.6 5 5 3 3 0 dual b/y bias 62.438992 0.891649 595.7906 1190.584 -0.0093 -7.8 -0.09999999999999964 5.83 4102.6 nuORFdb_human ENST00000537473.2_1_12:57065549-57066549:- ENST00000537473.2_1_12:57065549-57066549:- PTGES3 ncRNA Processed Transcript lncRNA GN=PTGES3 ncRNA Processed Transcript E_20221201_NO30_400nL_HLAc1_1e7_directIP_titration_rep1_Slot2-3_1_3512 10mio-rep2 E_20221201_NO30_400nL_HLAc1_1e7_directIP_titration_rep2_Slot2-3_1_3513.18020.1376.2 2 2 1 13.62 5.86 3.47 7.76 91.9 6 1 1 44400.0 Hydrogen 16 (E) 9 RTDQFYVVY (E) 9 NEPGVQPTSVY R T D\Q\F|Y|V|V\Y 66.7 6 6 3 3 0 dual b/y bias 62.245739 0.89134 595.7902 1190.584 -0.0113 -9.5 -2.8 5.83 4102.6 nuORFdb_human ENST00000537473.2_1_12:57065549-57066549:- ENST00000537473.2_1_12:57065549-57066549:- PTGES3 ncRNA Processed Transcript lncRNA GN=PTGES3 ncRNA Processed Transcript E_20221201_NO30_400nL_HLAc1_1e7_directIP_titration_rep2_Slot2-3_1_3513 10mio-rep3 E_20221201_NO30_400nL_HLAc1_1e7_directIP_titration_rep3_Slot2-3_1_3514.17976.1378.2 2 2 1 14.3 8.29 3.5 6.02 95.4 6 1 1 50100.0 Hydrogen 16 (E) 9 RTDQFYVVY (E) 9 FAWFTSAVVY R T D\Q\F\Y|V|V\Y 66.7 6 6 2 2 0 b-ion bias 62.095482 0.89036 595.7919 1190.584 -0.0073 -6.2 0.09999999999999964 5.83 4102.6 nuORFdb_human ENST00000537473.2_1_12:57065549-57066549:- ENST00000537473.2_1_12:57065549-57066549:- PTGES3 ncRNA Processed Transcript lncRNA GN=PTGES3 ncRNA Processed Transcript E_20221201_NO30_400nL_HLAc1_1e7_directIP_titration_rep3_Slot2-3_1_3514 20mio-rep1 E_20221201_NO30_400nL_HLAc1_2e7_directIP_titration_rep1_Slot2-4_1_3515.17965.1372.2 2 4 1 13.16 6.87 3.62 6.29 92.5 6 1 1 90300.0 Hydrogen 16 (E) 9 RTDQFYVVY (E) 9 FAWFTSAVVY R T D\Q\F|Y|V|V\Y 66.7 6 6 3 3 0 dual b/y bias 62.044025 0.893793 595.7918 1190.584 -0.0073 -6.2 0.0 5.83 4102.6 nuORFdb_human ENST00000537473.2_1_12:57065549-57066549:- ENST00000537473.2_1_12:57065549-57066549:- PTGES3 ncRNA Processed Transcript lncRNA GN=PTGES3 ncRNA Processed Transcript E_20221201_NO30_400nL_HLAc1_2e7_directIP_titration_rep1_Slot2-4_1_3515 20mio-rep2 E_20221201_NO30_400nL_HLAc1_2e7_directIP_titration_rep3_Slot2-4_1_3518.18042.1368.2 2 3 1 11.72 8.32 2.6 3.4 93.3 6 1 1 68600.0 Hydrogen 16 (E) 9 RTDQFYVVY (E) 9 FAWFTSAVVY R T D\Q\F\Y|V|V\Y 66.7 6 6 2 2 0 b-ion bias 62.318748 0.896592 595.7916 1190.584 -0.0073 -6.2 0.09999999999999964 5.83 4102.6 nuORFdb_human ENST00000537473.2_1_12:57065549-57066549:- ENST00000537473.2_1_12:57065549-57066549:- PTGES3 ncRNA Processed Transcript lncRNA GN=PTGES3 ncRNA Processed Transcript E_20221201_NO30_400nL_HLAc1_2e7_directIP_titration_rep3_Slot2-4_1_3518 20mio-rep3 E_20221201_NO30_400nL_HLAc1_2e7_directIP_titration_rep4_Slot2-4_1_3519.18009.1367.2 2 2 1 14.35 9.99 1.04 4.36 95.9 6 1 1 72000.0 Hydrogen 16 (E) 9 RTDQFYVVY (E) 9 FAWFTSAVVY R T D\Q\F\Y|V|V\Y 66.7 6 6 2 2 0 b-ion bias 62.200798 0.896927 595.7917 1190.584 -0.0073 -6.2 -0.40000000000000036 5.83 4102.6 nuORFdb_human ENST00000537473.2_1_12:57065549-57066549:- ENST00000537473.2_1_12:57065549-57066549:- PTGES3 ncRNA Processed Transcript lncRNA GN=PTGES3 ncRNA Processed Transcript E_20221201_NO30_400nL_HLAc1_2e7_directIP_titration_rep4_Slot2-4_1_3519 40mio-rep1 E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep1_Slot1-04_1_3496.18218.1333.2 2 5 1 16.43 12.0 4.03 4.43 98.1 6 1 1 278000.0 Hydrogen 16 (E) 9 RTDQFYVVY (E) 9 FAWFTSAVVY R T D\Q\F|Y|V|V\Y 66.7 6 6 3 3 0 dual b/y bias 62.931625 0.918397 595.7952 1190.584 -0.0013 -1.1 2.1 5.83 4102.6 nuORFdb_human ENST00000537473.2_1_12:57065549-57066549:- ENST00000537473.2_1_12:57065549-57066549:- PTGES3 ncRNA Processed Transcript lncRNA GN=PTGES3 ncRNA Processed Transcript E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep1_Slot1-04_1_3496 40mio-rep2 E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep2_Slot2-5_1_3520.17877.1349.2 2 5 1 16.34 9.81 4.01 6.53 96.1 6 1 1 151000.0 Hydrogen 16 (E) 9 RTDQFYVVY (E) 9 FAWFTSAVVY R T D\Q\F|Y|V|V\Y 66.7 6 6 3 3 0 dual b/y bias 61.755249 0.908221 595.7939 1190.584 -0.0033 -2.8 2.1000000000000005 5.83 4102.6 nuORFdb_human ENST00000537473.2_1_12:57065549-57066549:- ENST00000537473.2_1_12:57065549-57066549:- PTGES3 ncRNA Processed Transcript lncRNA GN=PTGES3 ncRNA Processed Transcript E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep2_Slot2-5_1_3520 40mio-rep3 E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep3_Slot2-5_1_3521.17921.1356.2 2 4 1 15.62 9.34 3.85 6.29 97.1 6 1 1 163000.0 Hydrogen 16 (E) 9 RTDQFYVVY (E) 9 FAWFTSAVVY R T D\Q\F|Y|V|V\Y 66.7 6 6 3 3 0 dual b/y bias 61.907726 0.903657 595.7938 1190.584 -0.0033 -2.8 1.7999999999999998 5.83 4102.6 nuORFdb_human ENST00000537473.2_1_12:57065549-57066549:- ENST00000537473.2_1_12:57065549-57066549:- PTGES3 ncRNA Processed Transcript lncRNA GN=PTGES3 ncRNA Processed Transcript E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep3_Slot2-5_1_3521 40mio-rep4 E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep4_Slot2-5_1_3522.17888.1353.2 2 5 1 16.37 9.32 4.14 7.04 96.7 6 1 1 157000.0 Hydrogen 16 (E) 9 RTDQFYVVY (E) 9 FAWFTSAVVY R T D\Q\F|Y|V|V\Y 66.7 6 6 3 3 0 dual b/y bias 61.776615 0.905521 595.7929 1190.584 -0.0053 -4.5 0.20000000000000018 5.83 4102.6 nuORFdb_human ENST00000537473.2_1_12:57065549-57066549:- ENST00000537473.2_1_12:57065549-57066549:- PTGES3 ncRNA Processed Transcript lncRNA GN=PTGES3 ncRNA Processed Transcript E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep4_Slot2-5_1_3522 5mio-rep1 E_20221201_NO30_400nL_HLAc1_5e6_directIP_titration_rep1_Slot1-37_1_3502.18303.1391.2 2 1 1 11.58 6.17 3.11 5.4 90.0 5 1 1 4290.0 Hydrogen 16 (E) 9 RTDQFYVVY (E) 9 FAWFTSAVVY R T D Q/F|Y|V|V\Y 55.6 5 4 4 3 0 dual b/y bias 63.219582 0.881903 595.7905 1190.584 -0.0113 -9.5 -2.5 5.83 4102.6 nuORFdb_human ENST00000537473.2_1_12:57065549-57066549:- ENST00000537473.2_1_12:57065549-57066549:- PTGES3 ncRNA Processed Transcript lncRNA GN=PTGES3 ncRNA Processed Transcript E_20221201_NO30_400nL_HLAc1_5e6_directIP_titration_rep1_Slot1-37_1_3502 5mio-rep2 E_20221201_NO30_400nL_HLAc1_5e6_directIP_titration_rep2_Slot2-2_1_3510.18189.1409.2 2 1 1 8.8 5.21 0.47 3.59 82.8 5 1 1 4400.0 Hydrogen 16 (E) 9 RTDQFYVVY (E) 9 YVVFnNGTLY R T D Q\F\Y\V|V\Y 55.6 5 5 1 1 0 b-ion bias 62.824295 0.870884 595.7894 1190.584 -0.0133 -11.2 -2.5 5.83 4102.6 nuORFdb_human ENST00000537473.2_1_12:57065549-57066549:- ENST00000537473.2_1_12:57065549-57066549:- PTGES3 ncRNA Processed Transcript lncRNA GN=PTGES3 ncRNA Processed Transcript E_20221201_NO30_400nL_HLAc1_5e6_directIP_titration_rep2_Slot2-2_1_3510 5mio-rep3 E_20221201_NO30_400nL_HLAc1_5e6_directIP_titration_rep3_Slot2-2_1_3511.18166.1384.2 2 1 1 12.55 7.21 4.62 5.34 89.7 6 1 1 4520.0 Hydrogen 16 (E) 9 RTDQFYVVY (E) 9 FAWFTSAVVY R T\D Q\F|Y\V|V\Y 55.6 5 6 2 2 0 b-ion bias 62.745831 0.886514 595.7898 1190.584 -0.0113 -9.5 -1.3000000000000007 5.83 4102.6 nuORFdb_human ENST00000537473.2_1_12:57065549-57066549:- ENST00000537473.2_1_12:57065549-57066549:- PTGES3 ncRNA Processed Transcript lncRNA GN=PTGES3 ncRNA Processed Transcript E_20221201_NO30_400nL_HLAc1_5e6_directIP_titration_rep3_Slot2-2_1_3511 20mio-rep3 E_20221201_NO30_400nL_HLAc1_2e7_directIP_titration_rep4_Slot2-4_1_3519.4753.1657.3 3 1 1 7.98 1.99 9.56 5.98 64.9 6 2 2 3880.0 M147m Hydrogen 140 (H) 9 RTEPAARmW (M) 9 APRETKDTDT R T E\P|A|A/R/m\W 66.7 6 4 4 2 0 unbiased m:Oxidized methionine 16.425259 0.713894 378.519 1117.557 15.9853 -8.5 -2.7 9.6 34254.2 nuORFdb_human ENST00000546155.5_1_19:41116125-41119449:+ ENST00000546155.5_1_19:41116125-41119449:+|ENST00000204005.13_1_19:41114149-41118026:+|ENST00000243562.13_1_19:41115651-41118026:+ LTBP4 ncRNA Retained Intron lncRNA GN=LTBP4 ncRNA Retained Intron E_20221201_NO30_400nL_HLAc1_2e7_directIP_titration_rep4_Slot2-4_1_3519 40mio-rep2 E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep2_Slot2-5_1_3520.3688.1620.3 3 1 1 13.12 13.12 13.12 78.7 6 1 1 9150.0 Hydrogen 45 (T) 9 RTHPLTHSW (N) 9 R T H|P/L/T\H|S\W 66.7 6 4 4 2 0 unbiased 12.75026 0.737741 378.8625 1134.58 -0.0089 -7.8 -2.8999999999999995 9.76 6068.7 nuORFdb_human ENST00000311713.11_1_11:62603300-62606661:- ENST00000311713.11_1_11:62603300-62606661:- WDR74 Out-of-Frame Out-of-Frame GN=WDR74 Out-of-Frame E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep2_Slot2-5_1_3520 40mio-rep3 E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep3_Slot2-5_1_3521.3724.1633.3 3 2 1 12.2 12.2 12.2 64.0 7 1 1 12600.0 Hydrogen 45 (T) 9 RTHPLTHSW (N) 9 R T/H|P/L/T|H|S\W 77.8 7 4 6 3 0 dual b/y bias 12.872449 0.72962 378.8632 1134.58 -0.0059 -5.2 -0.6000000000000005 9.76 6068.7 nuORFdb_human ENST00000311713.11_1_11:62603300-62606661:- ENST00000311713.11_1_11:62603300-62606661:- WDR74 Out-of-Frame Out-of-Frame GN=WDR74 Out-of-Frame E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep3_Slot2-5_1_3521 40mio-rep1 E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep1_Slot1-04_1_3496.3694.1613.3 3 1 1 10.23 3.32 8.25 6.9 75.7 6 1 1 3950.0 Hydrogen 45 (T) 10 RTHPLTHSWN (-) 10 HTREKPFGYN R T H|P/L/T H\S\W\N 50.0 5 4 3 1 0 unbiased 12.772222 0.742121 416.8769 1248.623 -0.0068 -5.4 -2.2 9.76 6068.7 nuORFdb_human ENST00000311713.11_1_11:62603300-62606661:- ENST00000311713.11_1_11:62603300-62606661:- WDR74 Out-of-Frame Out-of-Frame GN=WDR74 Out-of-Frame E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep1_Slot1-04_1_3496 40mio-rep3 E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep3_Slot2-5_1_3521.3448.1615.3 3 2 1 8.95 2.45 6.46 6.49 74.3 6 1 1 10500.0 Hydrogen 45 (T) 10 RTHPLTHSWN (-) 10 FVLSSALnPWN R T H|P L/T/H-S\W\N 50.0 5 3 3 1 1 unbiased 11.91886 0.740876 416.8758 1248.623 -0.0098 -7.8 -3.2 9.76 6068.7 nuORFdb_human ENST00000311713.11_1_11:62603300-62606661:- ENST00000311713.11_1_11:62603300-62606661:- WDR74 Out-of-Frame Out-of-Frame GN=WDR74 Out-of-Frame E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep3_Slot2-5_1_3521 40mio-rep1 E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep1_Slot1-04_1_3496.4688.1666.3 3 3 1 7.8 2.71 3.52 5.09 63.7 5 3 3 203000.0 Hydrogen 14 (R) 9 RTKGGRVLW (R) 9 SGIKTPKRW R T|K|G/G R\V L\W 33.3 3 4 3 2 0 unbiased 16.20381 0.708086 358.216 1072.637 -0.004 -3.7 -0.5 12.01 3749.3 nuORFdb_human ENST00000263754.4_1_3:20081618-20081714:+ ENST00000263754.4_1_3:20081618-20081714:+ KAT2B 5' uORF 5' uORF GN=KAT2B 5' uORF E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep1_Slot1-04_1_3496 40mio-rep2 E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep2_Slot2-5_1_3520.3383.1689.3 3 1 1 10.53 10.55 10.53 73.1 6 2 3 4740.0 Hydrogen 112 (A) 9 RTKPPPPKF (Y) 9 R T K|P|P|P/P\K\F 66.7 6 5 4 3 0 dual b/y bias 11.697568 0.693647 356.547 1067.636 -0.0096 -9.0 -4.1 11.17 14727.5 nuORFdb_human ENST00000376033.2_1_6:31591647-31593276:+ ENST00000376033.2_1_6:31591647-31593276:+ PRRC2A Out-of-Frame Out-of-Frame GN=PRRC2A Out-of-Frame E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep2_Slot2-5_1_3520 40mio-rep1 E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep1_Slot1-04_1_3496.13552.1321.2 2 1 1 10.0 8.85 4.1 1.15 76.2 6 2 2 7740.0 Hydrogen 83 (S) 9 RTLNLKAEW (I) 9 RRVWKAEW R T L\N\L\K|A\E\W 66.7 6 6 1 1 0 b-ion bias 46.813095 0.925483 565.8162 1130.632 -0.0069 -6.1 -2.8999999999999995 8.75 13400.6 nuORFdb_human TCONS_00011192_6:133421199-133427445:+ TCONS_00011192_6:133421199-133427445:+ linc-EYA4 lincRNA lncRNA GN=linc-EYA4 lincRNA E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep1_Slot1-04_1_3496 20mio-rep1 E_20221201_NO30_400nL_HLAc1_2e7_directIP_titration_rep1_Slot2-4_1_3515.8034.1652.3 3 1 1 9.52 6.13 4.41 3.39 68.1 6 2 2 9340.0 Hydrogen 53 (K) 10 RTLPGSARTW (R) 10 ELKPGLCSGAL R T L/P/G|S/A/R T\W 50.0 5 2 5 1 0 y-ion bias 27.753674 0.717074 382.2093 1144.622 -0.0097 -8.5 -2.3 12.0 9560.8 nuORFdb_human ENST00000355485.6_1_1:116202302-116206444:+ ENST00000355485.6_1_1:116202302-116206444:+ VANGL1 Out-of-Frame Out-of-Frame GN=VANGL1 Out-of-Frame E_20221201_NO30_400nL_HLAc1_2e7_directIP_titration_rep1_Slot2-4_1_3515 20mio-rep2 E_20221201_NO30_400nL_HLAc1_2e7_directIP_titration_rep3_Slot2-4_1_3518.8183.1655.3 3 3 1 9.76 7.77 4.5 1.99 68.7 6 2 2 17900.0 Hydrogen 53 (K) 10 RTLPGSARTW (R) 10 QLPGHSGPPVR R T L/P|G|S|A/R T-W 50.0 5 3 5 3 1 unbiased 28.269588 0.715428 382.208 1144.622 -0.0127 -11.1 -4.8 12.0 9560.8 nuORFdb_human ENST00000355485.6_1_1:116202302-116206444:+ ENST00000355485.6_1_1:116202302-116206444:+ VANGL1 Out-of-Frame Out-of-Frame GN=VANGL1 Out-of-Frame E_20221201_NO30_400nL_HLAc1_2e7_directIP_titration_rep3_Slot2-4_1_3518 20mio-rep3 E_20221201_NO30_400nL_HLAc1_2e7_directIP_titration_rep4_Slot2-4_1_3519.8064.1656.3 3 1 1 10.56 7.59 5.45 2.97 67.5 6 2 2 4260.0 Hydrogen 53 (K) 10 RTLPGSARTW (R) 10 QLPGHSGPPVR R T L/P/G|S|A/R T\W 50.0 5 3 5 2 0 unbiased 27.85619 0.714743 382.2091 1144.622 -0.0097 -8.5 -2.7 12.0 9560.8 nuORFdb_human ENST00000355485.6_1_1:116202302-116206444:+ ENST00000355485.6_1_1:116202302-116206444:+ VANGL1 Out-of-Frame Out-of-Frame GN=VANGL1 Out-of-Frame E_20221201_NO30_400nL_HLAc1_2e7_directIP_titration_rep4_Slot2-4_1_3519 40mio-rep2 E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep2_Slot2-5_1_3520.8021.1629.3 3 1 1 11.2 7.95 6.74 3.25 74.6 6 2 2 18200.0 Hydrogen 53 (K) 10 RTLPGSARTW (R) 10 QLPGHSGPPVR R T L/P/G|S|A/R T\W 50.0 5 3 5 2 0 unbiased 27.712824 0.73186 382.2097 1144.622 -0.0067 -5.9 -1.0 12.0 9560.8 nuORFdb_human ENST00000355485.6_1_1:116202302-116206444:+ ENST00000355485.6_1_1:116202302-116206444:+ VANGL1 Out-of-Frame Out-of-Frame GN=VANGL1 Out-of-Frame E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep2_Slot2-5_1_3520 40mio-rep3 E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep3_Slot2-5_1_3521.8036.1632.3 3 1 1 10.82 8.01 5.98 2.81 67.1 7 2 2 10800.0 Hydrogen 53 (K) 10 RTLPGSARTW (R) 10 QLPGHSGPPVR R T L/P/G|S|A/R\T\W 70.0 7 4 5 2 0 unbiased 27.761587 0.729746 382.2096 1144.622 -0.0067 -5.9 -1.3000000000000007 12.0 9560.8 nuORFdb_human ENST00000355485.6_1_1:116202302-116206444:+ ENST00000355485.6_1_1:116202302-116206444:+ VANGL1 Out-of-Frame Out-of-Frame GN=VANGL1 Out-of-Frame E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep3_Slot2-5_1_3521 40mio-rep4 E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep4_Slot2-5_1_3522.8045.1633.3 3 1 1 12.9 5.9 8.99 6.99 72.4 8 2 2 12500.0 Hydrogen 53 (K) 10 RTLPGSARTW (R) 10 QLPGHSGPPVR R T/L/P/G|S|A/R|T\W 80.0 8 4 7 3 0 unbiased 27.787804 0.729689 382.2095 1144.622 -0.0097 -8.5 -3.8 12.0 9560.8 nuORFdb_human ENST00000355485.6_1_1:116202302-116206444:+ ENST00000355485.6_1_1:116202302-116206444:+ VANGL1 Out-of-Frame Out-of-Frame GN=VANGL1 Out-of-Frame E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep4_Slot2-5_1_3522 20mio-rep2 E_20221201_NO30_400nL_HLAc1_2e7_directIP_titration_rep3_Slot2-4_1_3518.23123.1327.2 2 1 1 9.61 5.62 4.54 3.98 68.5 5 1 1 2070.0 Hydrogen 37 (C) 11 RTLTPVTPLSW (V) 11 RTRARQLPSW R T L T|P V\T|P L|S\W 27.3 3 5 3 3 0 unbiased 79.867195 0.92187 635.8577 1270.715 -0.0067 -5.3 1.0 9.75 7510.0 nuORFdb_human ENST00000413293.1_1_22:22661204-22661477:+ ENST00000413293.1_1_22:22661204-22661477:+ LL22NC03-80A10.6 Pseudogene Pseudogene GN=LL22NC03-80A10.6 Pseudogene E_20221201_NO30_400nL_HLAc1_2e7_directIP_titration_rep3_Slot2-4_1_3518 20mio-rep3 E_20221201_NO30_400nL_HLAc1_2e7_directIP_titration_rep4_Slot2-4_1_3519.23178.1317.2 2 1 1 10.56 7.44 5.62 3.13 76.5 5 1 1 2160.0 Hydrogen 37 (C) 11 RTLTPVTPLSW (V) 11 LLSSLGPTLPSW R T L T|P V|T|P L|S\W 27.3 3 5 4 4 0 dual b/y bias 80.048866 0.928284 635.8576 1270.715 -0.0067 -5.3 0.5 9.75 7510.0 nuORFdb_human ENST00000413293.1_1_22:22661204-22661477:+ ENST00000413293.1_1_22:22661204-22661477:+ LL22NC03-80A10.6 Pseudogene Pseudogene GN=LL22NC03-80A10.6 Pseudogene E_20221201_NO30_400nL_HLAc1_2e7_directIP_titration_rep4_Slot2-4_1_3519 40mio-rep1 E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep1_Slot1-04_1_3496.23406.1293.2 2 2 1 14.05 8.06 6.32 5.99 83.2 7 1 1 9670.0 Hydrogen 37 (C) 11 RTLTPVTPLSW (V) 11 LLSSLGPTLPSW R T L/T|P V|T|P|L|S\W 54.5 6 6 6 5 0 dual b/y bias 80.857864 0.943436 635.8581 1270.715 -0.0067 -5.3 -2.0999999999999996 9.75 7510.0 nuORFdb_human ENST00000413293.1_1_22:22661204-22661477:+ ENST00000413293.1_1_22:22661204-22661477:+ LL22NC03-80A10.6 Pseudogene Pseudogene GN=LL22NC03-80A10.6 Pseudogene E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep1_Slot1-04_1_3496 40mio-rep2 E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep2_Slot2-5_1_3520.23221.1311.2 2 2 1 12.87 7.85 6.27 5.02 77.3 7 1 1 6750.0 Hydrogen 37 (C) 11 RTLTPVTPLSW (V) 11 LLSSLGPTLPSW R T L T|P\V/T|P\L|S\W 63.6 7 6 4 3 0 unbiased 80.217514 0.932153 635.8584 1270.715 -0.0067 -5.3 -0.39999999999999947 9.75 7510.0 nuORFdb_human ENST00000413293.1_1_22:22661204-22661477:+ ENST00000413293.1_1_22:22661204-22661477:+ LL22NC03-80A10.6 Pseudogene Pseudogene GN=LL22NC03-80A10.6 Pseudogene E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep2_Slot2-5_1_3520 40mio-rep3 E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep3_Slot2-5_1_3521.23282.1322.2 2 3 1 12.97 7.49 6.45 5.48 77.2 6 1 1 8980.0 Hydrogen 37 (C) 11 RTLTPVTPLSW (V) 11 LEVKLGELPSW R T L T|P V|T|P\L|S\W 45.5 5 6 4 4 0 dual b/y bias 80.426376 0.924947 635.8581 1270.715 -0.0067 -5.3 -0.7000000000000002 9.75 7510.0 nuORFdb_human ENST00000413293.1_1_22:22661204-22661477:+ ENST00000413293.1_1_22:22661204-22661477:+ LL22NC03-80A10.6 Pseudogene Pseudogene GN=LL22NC03-80A10.6 Pseudogene E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep3_Slot2-5_1_3521 40mio-rep4 E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep4_Slot2-5_1_3522.23270.1321.2 2 1 1 9.7 6.62 4.21 3.08 74.2 6 1 1 5620.0 Hydrogen 37 (C) 11 RTLTPVTPLSW (V) 11 LLSSLGPTLPSW R T L\T|P V\T|P L|S\W 36.4 4 6 3 3 0 b-ion bias 80.373001 0.926053 635.8592 1270.715 -0.0047 -3.7 1.0 9.75 7510.0 nuORFdb_human ENST00000413293.1_1_22:22661204-22661477:+ ENST00000413293.1_1_22:22661204-22661477:+ LL22NC03-80A10.6 Pseudogene Pseudogene GN=LL22NC03-80A10.6 Pseudogene E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep4_Slot2-5_1_3522 40mio-rep4 E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep4_Slot2-5_1_3522.12838.1502.2 2 1 1 11.48 8.72 2.79 2.76 76.1 6 1 1 5380.0 Hydrogen 44 (W) 9 RTPGSLASW (L) 9 LGPTSRASW R T/P G\S\L|A|S\W 55.6 5 5 3 2 0 unbiased 44.333744 0.812428 487.7547 974.505 -0.0027 -2.8 1.9000000000000004 9.75 9981.2 nuORFdb_human ENST00000270747.7_1_1:16531910-16532549:- ENST00000270747.7_1_1:16531910-16532549:- ARHGEF19 Out-of-Frame Out-of-Frame GN=ARHGEF19 Out-of-Frame E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep4_Slot2-5_1_3522 20mio-rep1 E_20221201_NO30_400nL_HLAc1_2e7_directIP_titration_rep1_Slot2-4_1_3515.20728.1392.2 2 1 1 12.9 10.04 8.27 2.86 86.5 6 1 1 6950.0 Hydrogen 38 (P) 9 RTPNYVNFW (M) 9 MALASASVGGFW R T/P N\Y|V|N|F\W 55.6 5 5 4 3 0 dual b/y bias 71.581963 0.881632 598.7931 1196.585 -0.006 -5.0 1.2000000000000002 8.75 6555.3 nuORFdb_human ENST00000637919.1_2_20:8130974-8608998:+ ENST00000637919.1_2_20:8130974-8608998:+|ENST00000635830.1_1_20:8286572-8608998:+|ENST00000626114.2_1_20:8351996-8608998:+ PLCB1 Out-of-Frame Out-of-Frame GN=PLCB1 Out-of-Frame E_20221201_NO30_400nL_HLAc1_2e7_directIP_titration_rep1_Slot2-4_1_3515 20mio-rep3 E_20221201_NO30_400nL_HLAc1_2e7_directIP_titration_rep4_Slot2-4_1_3519.20799.1389.2 2 1 1 12.8 10.57 8.87 2.23 83.8 6 1 1 5190.0 Hydrogen 38 (P) 9 RTPNYVNFW (M) 9 MALASASVGGFW R T\P N\Y|V|N|F\W 55.6 5 6 3 3 0 dual b/y bias 71.836212 0.883441 598.7911 1196.585 -0.01 -8.4 -2.6000000000000005 8.75 6555.3 nuORFdb_human ENST00000637919.1_2_20:8130974-8608998:+ ENST00000637919.1_2_20:8130974-8608998:+|ENST00000635830.1_1_20:8286572-8608998:+|ENST00000626114.2_1_20:8351996-8608998:+ PLCB1 Out-of-Frame Out-of-Frame GN=PLCB1 Out-of-Frame E_20221201_NO30_400nL_HLAc1_2e7_directIP_titration_rep4_Slot2-4_1_3519 40mio-rep1 E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep1_Slot1-04_1_3496.21013.1357.2 2 2 1 12.82 9.53 6.96 3.29 77.4 6 1 1 24300.0 Hydrogen 38 (P) 9 RTPNYVNFW (M) 9 MALASASVGGFW R T|P N\Y|V|N|F\W 55.6 5 6 4 4 0 dual b/y bias 72.589714 0.903361 598.793 1196.585 -0.006 -5.0 -1.7999999999999998 8.75 6555.3 nuORFdb_human ENST00000637919.1_2_20:8130974-8608998:+ ENST00000637919.1_2_20:8130974-8608998:+|ENST00000635830.1_1_20:8286572-8608998:+|ENST00000626114.2_1_20:8351996-8608998:+ PLCB1 Out-of-Frame Out-of-Frame GN=PLCB1 Out-of-Frame E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep1_Slot1-04_1_3496 40mio-rep2 E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep2_Slot2-5_1_3520.14703.1328.2 2 1 1 11.41 8.46 1.47 2.95 70.4 7 1 1 9060.0 Hydrogen 78 (D) 11 RTSQTVTNLSF (-) 11 VTQGTTSVVQSF R T S Q\T\V|T|N\L|S\F 63.6 7 7 3 3 0 b-ion bias 50.790184 0.921276 627.3268 1253.648 -0.0017 -1.4 3.5000000000000004 9.75 9557.6 nuORFdb_human ENST00000393250.8_1_12:76844735-76953307:- ENST00000393250.8_1_12:76844735-76953307:- OSBPL8 5' Overlap uORF 5' Overlap uORF GN=OSBPL8 5' Overlap uORF E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep2_Slot2-5_1_3520 40mio-rep3 E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep3_Slot2-5_1_3521.14723.1315.2 2 2 1 15.69 9.72 6.19 5.97 81.2 7 1 1 13200.0 Hydrogen 78 (D) 11 RTSQTVTNLSF (-) 11 GASVFAGLQGLSF R T S Q\T\V|T\N|L|S\F 63.6 7 7 3 3 0 b-ion bias 50.861767 0.929602 627.3255 1253.648 -0.0057 -4.6 0.0 9.75 9557.6 nuORFdb_human ENST00000393250.8_1_12:76844735-76953307:- ENST00000393250.8_1_12:76844735-76953307:- OSBPL8 5' Overlap uORF 5' Overlap uORF GN=OSBPL8 5' Overlap uORF E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep3_Slot2-5_1_3521 40mio-rep4 E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep4_Slot2-5_1_3522.14714.1322.2 2 3 1 13.58 8.68 5.62 4.9 83.9 6 1 1 15400.0 Hydrogen 78 (D) 11 RTSQTVTNLSF (-) 11 GASVFAGLQGLSF R T S Q T\V|T\N\L|S\F 54.5 6 6 2 2 0 b-ion bias 50.816326 0.924864 627.3261 1253.648 -0.0037 -3.0 1.7000000000000002 9.75 9557.6 nuORFdb_human ENST00000393250.8_1_12:76844735-76953307:- ENST00000393250.8_1_12:76844735-76953307:- OSBPL8 5' Overlap uORF 5' Overlap uORF GN=OSBPL8 5' Overlap uORF E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep4_Slot2-5_1_3522 10mio-rep1 E_20221201_NO30_400nL_HLAc1_1e7_directIP_titration_rep1_Slot2-3_1_3512.8094.1538.2 2 1 1 12.87 7.56 5.43 5.31 89.9 5 1 1 3410.0 Hydrogen 79 (G) 9 RTSVTGSAW (G) 9 RTDKTSAW R T S V|T|G\S|A\W 55.6 5 5 3 3 0 dual b/y bias 27.967476 0.789287 482.7421 964.485 -0.0079 -8.2 -0.4999999999999991 9.75 11042.3 nuORFdb_human ENST00000462451.1_1_2:85818805-85820182:+ ENST00000462451.1_1_2:85818805-85820182:+ VAMP5 ncRNA Retained Intron lncRNA GN=VAMP5 ncRNA Retained Intron E_20221201_NO30_400nL_HLAc1_1e7_directIP_titration_rep1_Slot2-3_1_3512 10mio-rep2 E_20221201_NO30_400nL_HLAc1_1e7_directIP_titration_rep2_Slot2-3_1_3513.8040.1539.2 2 1 1 10.26 7.56 2.7 2.7 88.4 5 1 1 3240.0 Hydrogen 79 (G) 9 RTSVTGSAW (G) 9 DASTVKSAW R T S V|T|G\S|A\W 55.6 5 5 3 3 0 dual b/y bias 27.780525 0.789189 482.7438 964.485 -0.0039 -4.1 2.6000000000000005 9.75 11042.3 nuORFdb_human ENST00000462451.1_1_2:85818805-85820182:+ ENST00000462451.1_1_2:85818805-85820182:+ VAMP5 ncRNA Retained Intron lncRNA GN=VAMP5 ncRNA Retained Intron E_20221201_NO30_400nL_HLAc1_1e7_directIP_titration_rep2_Slot2-3_1_3513 20mio-rep1 E_20221201_NO30_400nL_HLAc1_2e7_directIP_titration_rep1_Slot2-4_1_3515.7968.1534.2 2 1 1 13.27 10.64 4.26 2.63 90.1 6 1 1 6660.0 Hydrogen 79 (G) 9 RTSVTGSAW (G) 9 QFSGLVQW R T S/V|T|G|S|A\W 66.7 6 5 5 4 0 dual b/y bias 27.525879 0.791791 482.7423 964.485 -0.0079 -8.2 -1.9999999999999991 9.75 11042.3 nuORFdb_human ENST00000462451.1_1_2:85818805-85820182:+ ENST00000462451.1_1_2:85818805-85820182:+ VAMP5 ncRNA Retained Intron lncRNA GN=VAMP5 ncRNA Retained Intron E_20221201_NO30_400nL_HLAc1_2e7_directIP_titration_rep1_Slot2-4_1_3515 20mio-rep2 E_20221201_NO30_400nL_HLAc1_2e7_directIP_titration_rep3_Slot2-4_1_3518.8141.1546.2 2 2 1 13.29 9.06 4.23 4.23 90.1 5 1 1 10300.0 Hydrogen 79 (G) 9 RTSVTGSAW (G) 9 DASTVKSAW R T S V|T|G\S|A\W 55.6 5 5 3 3 0 dual b/y bias 28.124628 0.784428 482.7436 964.485 -0.0039 -4.1 2.2 9.75 11042.3 nuORFdb_human ENST00000462451.1_1_2:85818805-85820182:+ ENST00000462451.1_1_2:85818805-85820182:+ VAMP5 ncRNA Retained Intron lncRNA GN=VAMP5 ncRNA Retained Intron E_20221201_NO30_400nL_HLAc1_2e7_directIP_titration_rep3_Slot2-4_1_3518 20mio-rep3 E_20221201_NO30_400nL_HLAc1_2e7_directIP_titration_rep4_Slot2-4_1_3519.8006.1534.2 2 2 1 11.21 8.4 2.22 2.81 87.4 5 1 1 12500.0 Hydrogen 79 (G) 9 RTSVTGSAW (G) 9 FGLVQSGAW R T S V|T|G\S|A\W 55.6 5 5 3 3 0 dual b/y bias 27.65601 0.792279 482.7433 964.485 -0.0059 -6.2 -0.40000000000000036 9.75 11042.3 nuORFdb_human ENST00000462451.1_1_2:85818805-85820182:+ ENST00000462451.1_1_2:85818805-85820182:+ VAMP5 ncRNA Retained Intron lncRNA GN=VAMP5 ncRNA Retained Intron E_20221201_NO30_400nL_HLAc1_2e7_directIP_titration_rep4_Slot2-4_1_3519 40mio-rep1 E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep1_Slot1-04_1_3496.8254.1516.2 2 1 1 13.27 9.11 4.16 4.16 90.1 5 1 1 9290.0 Hydrogen 79 (G) 9 RTSVTGSAW (G) 9 QFSGLVQW R T S V|T|G|S|A\W 55.6 5 5 4 4 0 dual b/y bias 28.518139 0.803185 482.7446 964.485 -0.0019 -2.0 1.2000000000000002 9.75 11042.3 nuORFdb_human ENST00000462451.1_1_2:85818805-85820182:+ ENST00000462451.1_1_2:85818805-85820182:+ VAMP5 ncRNA Retained Intron lncRNA GN=VAMP5 ncRNA Retained Intron E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep1_Slot1-04_1_3496 40mio-rep2 E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep2_Slot2-5_1_3520.7958.1523.2 2 1 1 10.69 7.26 2.65 3.43 86.0 5 1 1 8390.0 Hydrogen 79 (G) 9 RTSVTGSAW (G) 9 SRLSTGASAD R T S V|T\G\S|A\W 55.6 5 5 2 2 0 b-ion bias 27.49539 0.79891 482.7423 964.485 -0.0079 -8.2 -3.299999999999999 9.75 11042.3 nuORFdb_human ENST00000462451.1_1_2:85818805-85820182:+ ENST00000462451.1_1_2:85818805-85820182:+ VAMP5 ncRNA Retained Intron lncRNA GN=VAMP5 ncRNA Retained Intron E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep2_Slot2-5_1_3520 40mio-rep3 E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep3_Slot2-5_1_3521.7971.1518.2 2 1 1 13.13 8.3 4.83 4.83 86.1 5 1 1 10500.0 Hydrogen 79 (G) 9 RTSVTGSAW (G) 9 RTDKTSAW R T S V|T|G\S|A\W 55.6 5 5 3 3 0 dual b/y bias 27.537233 0.802301 482.7428 964.485 -0.0059 -6.2 -1.6000000000000005 9.75 11042.3 nuORFdb_human ENST00000462451.1_1_2:85818805-85820182:+ ENST00000462451.1_1_2:85818805-85820182:+ VAMP5 ncRNA Retained Intron lncRNA GN=VAMP5 ncRNA Retained Intron E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep3_Slot2-5_1_3521 40mio-rep4 E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep4_Slot2-5_1_3522.7984.1523.2 2 1 1 15.36 10.54 5.83 4.82 93.7 6 1 1 11400.0 Hydrogen 79 (G) 9 RTSVTGSAW (G) 9 RTDKTSAW R T/S V|T|G|S|A\W 55.6 5 5 5 4 0 dual b/y bias 27.577276 0.799184 482.7431 964.485 -0.0059 -6.2 -1.5 9.75 11042.3 nuORFdb_human ENST00000462451.1_1_2:85818805-85820182:+ ENST00000462451.1_1_2:85818805-85820182:+ VAMP5 ncRNA Retained Intron lncRNA GN=VAMP5 ncRNA Retained Intron E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep4_Slot2-5_1_3522 40mio-rep1 E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep1_Slot1-04_1_3496.5265.1568.3 3 1 1 14.18 6.63 7.56 7.56 68.9 8 2 2 5730.0 Hydrogen 78 (C) 11 RTTPGCSARTW (-) 11 PQGIHSCPSPGR R T T|P/G|C|S/A/R\T\W 72.7 8 5 6 3 0 unbiased Carbamidomethylation C:Carbamidomethylation, 18.198437 0.7708 431.5433 1292.616 -0.002 -1.5 1.7000000000000002 12.0 9853.0 nuORFdb_human ENST00000393896.6_1_17:40068785-40075203:- ENST00000393896.6_1_17:40068785-40075203:-|ENST00000352035.6_1_17:40068785-40075198:- ACLY 5' Overlap uORF 5' Overlap uORF GN=ACLY 5' Overlap uORF E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep1_Slot1-04_1_3496 40mio-rep2 E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep2_Slot2-5_1_3520.5021.1576.3 3 2 1 16.7 8.59 8.11 8.11 69.8 8 2 2 14000.0 Hydrogen 78 (C) 11 RTTPGCSARTW (-) 11 PQGIHSCPSPGR R T T|P/G|C|S|A/R\T\W 72.7 8 6 6 4 0 dual b/y bias Carbamidomethylation C:Carbamidomethylation, 17.352789 0.765771 431.5408 1292.616 -0.008 -6.2 -1.2999999999999998 12.0 9853.0 nuORFdb_human ENST00000393896.6_1_17:40068785-40075203:- ENST00000393896.6_1_17:40068785-40075203:-|ENST00000352035.6_1_17:40068785-40075198:- ACLY 5' Overlap uORF 5' Overlap uORF GN=ACLY 5' Overlap uORF E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep2_Slot2-5_1_3520 40mio-rep3 E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep3_Slot2-5_1_3521.5041.1575.3 3 3 1 12.29 4.38 8.42 7.92 61.2 8 2 2 21400.0 Hydrogen 78 (C) 11 RTTPGCSARTW (-) 11 PQGIHSCPSPGR R T T|P/G|C|S/A/R|T\W 72.7 8 5 7 4 0 dual b/y bias Carbamidomethylation C:Carbamidomethylation, 17.41939 0.766074 431.5419 1292.616 -0.005 -3.9 0.6999999999999997 12.0 9853.0 nuORFdb_human ENST00000393896.6_1_17:40068785-40075203:- ENST00000393896.6_1_17:40068785-40075203:-|ENST00000352035.6_1_17:40068785-40075198:- ACLY 5' Overlap uORF 5' Overlap uORF GN=ACLY 5' Overlap uORF E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep3_Slot2-5_1_3521 40mio-rep4 E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep4_Slot2-5_1_3522.5049.1593.3 3 2 1 12.69 6.94 6.63 5.75 66.8 7 2 2 13600.0 Hydrogen 78 (C) 11 RTTPGCSARTW (-) 11 PQGIHSCPSPGR R T T|P/G|C|S/A/R T\W 54.5 6 4 6 3 0 unbiased Carbamidomethylation C:Carbamidomethylation, 17.444883 0.754956 431.54 1292.616 -0.011 -8.5 -3.8 12.0 9853.0 nuORFdb_human ENST00000393896.6_1_17:40068785-40075203:- ENST00000393896.6_1_17:40068785-40075203:-|ENST00000352035.6_1_17:40068785-40075198:- ACLY 5' Overlap uORF 5' Overlap uORF GN=ACLY 5' Overlap uORF E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep4_Slot2-5_1_3522 40mio-rep4 E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep4_Slot2-5_1_3522.2618.1667.3 3 2 1 13.06 2.14 8.88 10.92 75.8 6 2 2 9300.0 M13m Hydrogen 8 (S) 9 RTTSAmRTF (A) 9 GQAGANAALAGW R T T/S|A|m|R\T\F 66.7 6 5 4 3 0 dual b/y bias m:Oxidized methionine 9.049322 0.707983 362.8484 1070.541 15.9883 -6.1 -1.3999999999999995 12.0 3857.3 nuORFdb_human ENST00000537042.5_1_11:67209948-67210059:- ENST00000537042.5_1_11:67209948-67210059:- CORO1B Out-of-Frame Out-of-Frame GN=CORO1B Out-of-Frame E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep4_Slot2-5_1_3522 40mio-rep1 E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep1_Slot1-04_1_3496.14406.1369.2 2 1 1 16.74 14.44 7.39 2.3 94.4 7 1 1 12100.0 Hydrogen 27 (H) 11 RTWDAGGSAAW (S) 11 ALCVEGGGGTAW R T W D\A|G|G|S\A|A\W 63.6 7 7 4 4 0 dual b/y bias 49.762993 0.895738 589.2713 1177.539 -0.0038 -3.2 0.0 5.84 8556.7 nuORFdb_human ENST00000589218.5_1_19:37808810-37809047:+ ENST00000589218.5_1_19:37808810-37809047:+ HKR1 ncRNA Processed Transcript lncRNA GN=HKR1 ncRNA Processed Transcript E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep1_Slot1-04_1_3496 40mio-rep3 E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep3_Slot2-5_1_3521.14097.1383.2 2 1 1 13.63 11.16 6.04 2.46 81.9 7 1 1 14600.0 Hydrogen 27 (H) 11 RTWDAGGSAAW (S) 11 ALCVEGGGGTAW R T W D\A|G/G\S|A|A\W 63.6 7 6 4 3 0 unbiased 48.700169 0.886953 589.2696 1177.539 -0.0058 -4.9 -0.3000000000000007 5.84 8556.7 nuORFdb_human ENST00000589218.5_1_19:37808810-37809047:+ ENST00000589218.5_1_19:37808810-37809047:+ HKR1 ncRNA Processed Transcript lncRNA GN=HKR1 ncRNA Processed Transcript E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep3_Slot2-5_1_3521 40mio-rep4 E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep4_Slot2-5_1_3522.14084.1384.2 2 1 1 9.67 8.04 2.16 1.63 73.7 6 1 1 14100.0 Hydrogen 27 (H) 11 RTWDAGGSAAW (S) 11 QFAPDREEW R T W D\A\G\G S|A|A\W 45.5 5 6 2 2 0 b-ion bias 48.641651 0.886215 589.2695 1177.539 -0.0058 -4.9 -0.20000000000000018 5.84 8556.7 nuORFdb_human ENST00000589218.5_1_19:37808810-37809047:+ ENST00000589218.5_1_19:37808810-37809047:+ HKR1 ncRNA Processed Transcript lncRNA GN=HKR1 ncRNA Processed Transcript E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep4_Slot2-5_1_3522 40mio-rep3 E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep3_Slot2-5_1_3521.13101.1493.2 2 1 1 14.3 8.45 5.26 5.85 82.8 7 1 1 5350.0 Hydrogen 135 (G) 9 RTWSASAGW (C) 9 GTKCEIAGW R T/W\S|A|S|A|G|W 77.8 7 6 6 5 0 dual b/y bias 45.26123 0.818123 511.2438 1021.485 -0.0043 -4.2 0.39999999999999947 9.75 18005.1 nuORFdb_human ENST00000533683.6_2_19:14201006-14201489:- ENST00000533683.6_2_19:14201006-14201489:- SAMD1 5' Overlap uORF 5' Overlap uORF GN=SAMD1 5' Overlap uORF E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep3_Slot2-5_1_3521 40mio-rep4 E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep4_Slot2-5_1_3522.13099.1492.2 2 1 1 10.37 7.22 1.67 3.15 75.8 5 1 1 6020.0 Hydrogen 135 (G) 9 RTWSASAGW (C) 9 GTKCEIAGW R T W S|A|S|A|G\W 55.6 5 5 4 4 0 dual b/y bias 45.23476 0.818477 511.2417 1021.485 -0.0083 -8.1 -3.3999999999999995 9.75 18005.1 nuORFdb_human ENST00000533683.6_2_19:14201006-14201489:- ENST00000533683.6_2_19:14201006-14201489:- SAMD1 5' Overlap uORF 5' Overlap uORF GN=SAMD1 5' Overlap uORF E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep4_Slot2-5_1_3522 40mio-rep1 E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep1_Slot1-04_1_3496.18676.1380.2 2 1 1 13.55 9.77 3.25 3.78 91.3 6 1 1 10200.0 Hydrogen 9 (P) 11 RVAGDGTPVLW (L) 11 QVAWATVPLW R V A G D\G\T|P\V|L\W 54.5 6 6 2 2 0 b-ion bias 64.516655 0.889047 585.8144 1170.627 -0.0059 -5.0 -1.7999999999999998 5.84 3322.7 smORF_v4|nuORFdb_human PSMC5_17:61905106-61905210:+ PSMC5_17:61905106-61905210:+|ENST00000582420.1_1_17:61905093-61905207:+ PSMC5 uORF 17:61905106-61905210:+ 17 PSMC5 5' uORF 5' uORF GN=PSMC5 E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep1_Slot1-04_1_3496 10mio-rep2 E_20221201_NO30_400nL_HLAc1_1e7_directIP_titration_rep2_Slot2-3_1_3513.6858.1394.2 2 1 1 8.97 6.16 3.55 2.81 69.1 6 1 1 5700.0 Hydrogen 62 (S) 9 RVEEAIHPV (-) 9 EEVRLAHVP R V E\E|A\I|H\P\V 66.7 6 6 2 2 0 b-ion bias 23.698259 0.880156 525.2866 1049.574 -0.0071 -6.8 -0.09999999999999964 5.4 8070.2 nuORFdb_human ENST00000367590.8_1_1:180601192-180601402:+ ENST00000367590.8_1_1:180601192-180601402:+ XPR1 5' Overlap uORF 5' Overlap uORF GN=XPR1 5' Overlap uORF E_20221201_NO30_400nL_HLAc1_1e7_directIP_titration_rep2_Slot2-3_1_3513 20mio-rep1 E_20221201_NO30_400nL_HLAc1_2e7_directIP_titration_rep1_Slot2-4_1_3515.6758.1381.2 2 1 1 10.83 8.21 1.82 2.62 81.1 5 1 1 7560.0 Hydrogen 62 (S) 9 RVEEAIHPV (-) 9 IIPSGTEHPV R V E E|A\I|H|P\V 55.6 5 5 3 3 0 dual b/y bias 23.348387 0.888305 525.2866 1049.574 -0.0071 -6.8 -0.5999999999999996 5.4 8070.2 nuORFdb_human ENST00000367590.8_1_1:180601192-180601402:+ ENST00000367590.8_1_1:180601192-180601402:+ XPR1 5' Overlap uORF 5' Overlap uORF GN=XPR1 5' Overlap uORF E_20221201_NO30_400nL_HLAc1_2e7_directIP_titration_rep1_Slot2-4_1_3515 20mio-rep3 E_20221201_NO30_400nL_HLAc1_2e7_directIP_titration_rep4_Slot2-4_1_3519.6812.1382.2 2 1 1 10.16 9.11 2.08 1.05 80.7 5 1 1 11700.0 Hydrogen 62 (S) 9 RVEEAIHPV (-) 9 LRnELAHPV R V E|E A\I|H\P\V 44.4 4 5 2 2 0 b-ion bias 23.534105 0.887428 525.2873 1049.574 -0.0071 -6.8 -1.0 5.4 8070.2 nuORFdb_human ENST00000367590.8_1_1:180601192-180601402:+ ENST00000367590.8_1_1:180601192-180601402:+ XPR1 5' Overlap uORF 5' Overlap uORF GN=XPR1 5' Overlap uORF E_20221201_NO30_400nL_HLAc1_2e7_directIP_titration_rep4_Slot2-4_1_3519 40mio-rep1 E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep1_Slot1-04_1_3496.7077.1360.2 2 1 1 11.95 9.26 2.59 2.69 90.8 5 1 1 12100.0 Hydrogen 62 (S) 9 RVEEAIHPV (-) 9 PEKGGTKHPV R V E E|A\I|H|P\V 55.6 5 5 3 3 0 dual b/y bias 24.453796 0.901642 525.2883 1049.574 -0.0051 -4.9 -1.7000000000000002 5.4 8070.2 nuORFdb_human ENST00000367590.8_1_1:180601192-180601402:+ ENST00000367590.8_1_1:180601192-180601402:+ XPR1 5' Overlap uORF 5' Overlap uORF GN=XPR1 5' Overlap uORF E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep1_Slot1-04_1_3496 40mio-rep3 E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep3_Slot2-5_1_3521.6779.1370.2 2 1 1 12.12 7.62 2.58 4.49 87.7 6 1 1 23600.0 Hydrogen 62 (S) 9 RVEEAIHPV (-) 9 DQVKLNHPV R V E\E\A\I|H|P\V 66.7 6 6 2 2 0 b-ion bias 23.421003 0.895207 525.288 1049.574 -0.0051 -4.9 -0.3000000000000007 5.4 8070.2 nuORFdb_human ENST00000367590.8_1_1:180601192-180601402:+ ENST00000367590.8_1_1:180601192-180601402:+ XPR1 5' Overlap uORF 5' Overlap uORF GN=XPR1 5' Overlap uORF E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep3_Slot2-5_1_3521 40mio-rep4 E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep4_Slot2-5_1_3522.6776.1368.2 2 2 1 12.01 9.3 2.11 2.7 92.7 6 1 1 37500.0 Hydrogen 62 (S) 9 RVEEAIHPV (-) 9 GRSGSPRHPV R V E\E\A\I|H|P\V 66.7 6 6 2 2 0 b-ion bias 23.406376 0.896645 525.2884 1049.574 -0.0051 -4.9 -0.20000000000000018 5.4 8070.2 nuORFdb_human ENST00000367590.8_1_1:180601192-180601402:+ ENST00000367590.8_1_1:180601192-180601402:+ XPR1 5' Overlap uORF 5' Overlap uORF GN=XPR1 5' Overlap uORF E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep4_Slot2-5_1_3522 40mio-rep1 E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep1_Slot1-04_1_3496.21274.1352.2 2 3 1 10.11 3.9 5.67 6.21 76.9 5 1 1 15300.0 Hydrogen 37 (S) 11 RVITAGLPATW (H) 11 ASFPAAQIPIAV R V I\T A G\L|P A|T\W 27.3 3 5 2 2 0 b-ion bias 73.490936 0.906518 592.8411 1184.679 -0.0039 -3.3 -0.09999999999999964 9.75 7248.1 nuORFdb_human ENST00000391919.1_1_19:46857064-46878873:+ ENST00000391919.1_1_19:46857064-46878873:+ PPP5C 5' Overlap uORF 5' Overlap uORF GN=PPP5C 5' Overlap uORF E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep1_Slot1-04_1_3496 Eva-50mio-3fr AL20210827_EV_HLA_PDAC_PANFRO413_2D_50e6_W632_classI_fxn3.16406.16406.3 3 1 1 11.46 9.93 11.46 62.5 7 1 1 5830000.0 M9m Hydrogen 7 (L) 9 RVmHAYHLF (Q) 9 R V\m\H\A\Y\H\L/F 77.8 7 6 1 0 0 b-ion bias m:Oxidized methionine 0.939 100.0 31.35 100.0 0.0 397.2022 1173.599 15.9936 -1.1 -1.6 8.76 2462.0 nuORFdb_human ENST00000613675.4_1_17:36455328-36455388:+ ENST00000613675.4_1_17:36455328-36455388:+ MRPL45 Out-of-Frame Out-of-Frame GN=MRPL45 Out-of-Frame AL20210827_EV_HLA_PDAC_PANFRO413_2D_50e6_W632_classI_fxn3 40mio-rep3 E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep3_Slot2-5_1_3521.14887.1424.2 2 1 1 9.7 7.49 6.53 2.21 73.0 5 1 1 10200.0 Hydrogen 39 (A) 10 RVSPAAAPYF (-) 10 KITYFPYF R V S P A/A|A|P|Y\F 50.0 5 4 4 3 0 unbiased 51.427898 0.861146 539.7841 1078.568 -0.0073 -6.8 -2.2 8.75 4903.5 nuORFdb_human ENST00000409972.5_1_2:161264245-161264389:- ENST00000409972.5_1_2:161264245-161264389:- RBMS1 5' uORF 5' uORF GN=RBMS1 5' uORF E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep3_Slot2-5_1_3521 40mio-rep4 E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep4_Slot2-5_1_3522.14873.1428.2 2 1 1 9.27 5.4 5.0 3.87 76.1 5 1 1 8330.0 Hydrogen 39 (A) 10 RVSPAAAPYF (-) 10 KITYFPYF R V S P A\A|A|P|Y\F 50.0 5 5 3 3 0 unbiased 51.365147 0.858805 539.7868 1078.568 -0.0013 -1.2 3.5 8.75 4903.5 nuORFdb_human ENST00000409972.5_1_2:161264245-161264389:- ENST00000409972.5_1_2:161264245-161264389:- RBMS1 5' uORF 5' uORF GN=RBMS1 5' uORF E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep4_Slot2-5_1_3522 20mio-rep1 E_20221201_NO30_400nL_HLAc1_2e7_directIP_titration_rep1_Slot2-4_1_3515.15385.1374.2 2 1 1 8.13 3.32 5.71 4.81 60.1 7 1 1 6010.0 Hydrogen 44 (G) 11 RVSVAQAGVQW (R) 11 GAQEALAKNLW R V S V\A\Q|A\G\V|Q\W 63.6 7 7 2 2 0 b-ion bias 53.135384 0.892856 600.8241 1200.648 -0.0077 -6.4 -0.20000000000000018 9.75 9124.2 nuORFdb_human ENST00000279804.2_1_16:30913893-30914157:+ ENST00000279804.2_1_16:30913893-30914157:+ CTF1 3' dORF 3' dORF GN=CTF1 3' dORF E_20221201_NO30_400nL_HLAc1_2e7_directIP_titration_rep1_Slot2-4_1_3515 40mio-rep1 E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep1_Slot1-04_1_3496.15549.1350.2 2 1 1 13.64 5.57 9.04 8.07 76.1 7 1 1 13500.0 Hydrogen 44 (G) 11 RVSVAQAGVQW (R) 11 NAVQTVVRQGE R V S V|A\Q|A|G\V|Q\W 63.6 7 7 4 4 0 dual b/y bias 53.710823 0.907781 600.826 1200.648 -0.0037 -3.0 0.20000000000000018 9.75 9124.2 nuORFdb_human ENST00000279804.2_1_16:30913893-30914157:+ ENST00000279804.2_1_16:30913893-30914157:+ CTF1 3' dORF 3' dORF GN=CTF1 3' dORF E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep1_Slot1-04_1_3496 40mio-rep4 E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep4_Slot2-5_1_3522.15291.1357.2 2 1 1 12.52 4.84 8.96 7.67 76.4 7 1 1 5370.0 Hydrogen 44 (G) 11 RVSVAQAGVQW (R) 11 VFVLYFVQW R V S V|A|Q|A\G\V|Q\W 63.6 7 7 4 4 0 dual b/y bias 52.808056 0.903187 600.8253 1200.648 -0.0057 -4.7 0.0 9.75 9124.2 nuORFdb_human ENST00000279804.2_1_16:30913893-30914157:+ ENST00000279804.2_1_16:30913893-30914157:+ CTF1 3' dORF 3' dORF GN=CTF1 3' dORF E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep4_Slot2-5_1_3522 40mio-rep1 E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep1_Slot1-04_1_3496.4511.1656.3 3 1 1 9.22 2.9 10.54 6.32 56.5 7 2 2 2260.0 Hydrogen 31 (R) 11 RVTNGCQTRTW (C) 11 QRTAAYGHFGRD R V/T N\G|C|Q|T R|T\W 45.5 5 6 5 4 0 dual b/y bias Carbamidomethylation C:Carbamidomethylation, 15.592867 0.714984 460.2235 1378.664 -0.007 -5.1 -1.8999999999999995 12.0 7741.8 nuORFdb_human ENST00000432590.5_2_10:104181253-104181715:+ ENST00000432590.5_2_10:104181253-104181715:+ FBXL15 Out-of-Frame Out-of-Frame GN=FBXL15 Out-of-Frame E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep1_Slot1-04_1_3496 40mio-rep1 E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep1_Slot1-04_1_3496.9022.1561.3 3 2 1 9.24 0.31 7.62 8.94 68.0 6 3 3 60000.0 Hydrogen 83 (K) 10 RVVAKAWRSW (-) 10 VIRGKQLRCE R V V\A\K|A|W/R\S W 50.0 5 5 3 2 0 unbiased 31.169218 0.775076 420.2414 1258.717 -0.0083 -6.6 -3.3999999999999995 12.01 10538.0 nuORFdb_human ENST00000576722.5_1_17:1456361-1466031:- ENST00000576722.5_1_17:1456361-1466031:-|ENST00000576761.5_1_17:1456361-1461827:- PITPNA 5' uORF 5' uORF GN=PITPNA 5' uORF E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep1_Slot1-04_1_3496 20mio-rep1 E_20221201_NO30_400nL_HLAc1_2e7_directIP_titration_rep1_Slot2-4_1_3515.21066.1303.2 2 1 1 13.58 10.39 4.48 3.19 79.1 7 1 1 3750.0 Hydrogen 10 (S) 11 RVWQDAELGLY (F) 11 qDPYIRELGLY R V W|Q D\A\E|L|G\L\Y 54.5 6 7 3 3 0 b-ion bias 72.748848 0.93716 675.3435 1349.685 -0.0041 -3.0 3.2 4.37 3793.1 nuORFdb_human ENST00000568282.1_1_16:29819940-29820048:+ ENST00000568282.1_1_16:29819940-29820048:+ MAZ 5' Overlap uORF 5' Overlap uORF GN=MAZ 5' Overlap uORF E_20221201_NO30_400nL_HLAc1_2e7_directIP_titration_rep1_Slot2-4_1_3515 20mio-rep2 E_20221201_NO30_400nL_HLAc1_2e7_directIP_titration_rep3_Slot2-4_1_3518.21105.1299.2 2 3 1 11.69 9.81 3.17 1.88 83.6 7 1 1 8910.0 Hydrogen 10 (S) 11 RVWQDAELGLY (F) 11 qDPYIRELGLY R V W\Q D\A\E|L\G|L\Y 54.5 6 7 2 2 0 b-ion bias 72.900162 0.939756 675.341 1349.685 -0.0101 -7.5 -1.2000000000000002 4.37 3793.1 nuORFdb_human ENST00000568282.1_1_16:29819940-29820048:+ ENST00000568282.1_1_16:29819940-29820048:+ MAZ 5' Overlap uORF 5' Overlap uORF GN=MAZ 5' Overlap uORF E_20221201_NO30_400nL_HLAc1_2e7_directIP_titration_rep3_Slot2-4_1_3518 40mio-rep1 E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep1_Slot1-04_1_3496.21286.1261.2 2 2 1 14.43 11.5 2.26 2.93 82.8 7 1 1 6010.0 Hydrogen 10 (S) 11 RVWQDAELGLY (F) 11 qDPYIRELGLY R V W\Q D\A\E|L|G|L\Y 54.5 6 7 3 3 0 b-ion bias 73.532364 0.963076 675.3456 1349.685 -0.0001 -0.1 3.1 4.37 3793.1 nuORFdb_human ENST00000568282.1_1_16:29819940-29820048:+ ENST00000568282.1_1_16:29819940-29820048:+ MAZ 5' Overlap uORF 5' Overlap uORF GN=MAZ 5' Overlap uORF E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep1_Slot1-04_1_3496 40mio-rep2 E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep2_Slot2-5_1_3520.21176.1278.2 2 3 1 14.45 10.06 5.96 4.39 86.0 8 1 1 15600.0 Hydrogen 10 (S) 11 RVWQDAELGLY (F) 11 qDPYIRELGLY R V W\Q\D\A\E|L\G|L\Y 72.7 8 8 2 2 0 b-ion bias 73.154984 0.952674 675.341 1349.685 -0.0101 -7.5 -2.5999999999999996 4.37 3793.1 nuORFdb_human ENST00000568282.1_1_16:29819940-29820048:+ ENST00000568282.1_1_16:29819940-29820048:+ MAZ 5' Overlap uORF 5' Overlap uORF GN=MAZ 5' Overlap uORF E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep2_Slot2-5_1_3520 40mio-rep3 E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep3_Slot2-5_1_3521.21198.1282.2 2 2 1 12.63 10.46 1.62 2.17 77.4 6 1 1 7010.0 Hydrogen 10 (S) 11 RVWQDAELGLY (F) 11 TVDIQPACLGLY R V W Q\D A\E|L|G|L\Y 45.5 5 6 3 3 0 b-ion bias 73.22744 0.94991 675.3425 1349.685 -0.0061 -4.5 0.09999999999999964 4.37 3793.1 nuORFdb_human ENST00000568282.1_1_16:29819940-29820048:+ ENST00000568282.1_1_16:29819940-29820048:+ MAZ 5' Overlap uORF 5' Overlap uORF GN=MAZ 5' Overlap uORF E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep3_Slot2-5_1_3521 40mio-rep4 E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep4_Slot2-5_1_3522.21231.1279.2 2 2 1 13.88 11.75 4.3 2.13 84.8 7 1 1 8930.0 Hydrogen 10 (S) 11 RVWQDAELGLY (F) 11 qDPYIRELGLY R V W\Q D\A|E|L|G|L\Y 54.5 6 7 4 4 0 dual b/y bias 73.324936 0.951989 675.3427 1349.685 -0.0061 -4.5 0.20000000000000018 4.37 3793.1 nuORFdb_human ENST00000568282.1_1_16:29819940-29820048:+ ENST00000568282.1_1_16:29819940-29820048:+ MAZ 5' Overlap uORF 5' Overlap uORF GN=MAZ 5' Overlap uORF E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep4_Slot2-5_1_3522 Eva-50mio-3fr AL20210827_EV_HLA_PDAC_PANFRO413_2D_50e6_W632_classI_fxn3.22908.23006.2 2 2 1 11.31 5.7 4.31 5.61 86.8 5 1 1 105000000.0 Hydrogen 33 (G) 9 RWGPVAHPF (R) 9 TmQSLVKPF R\W G P V\A\H|P/F 55.6 5 4 2 1 0 b-ion bias 0.987 100.0 42.8 32.16 0.0 533.7831 1066.558 0.0008 0.7 0.19999999999999996 9.75 7318.4 nuORFdb_human ENST00000353704.2_2_9:35732632-35732818:+ ENST00000353704.2_2_9:35732632-35732818:+ CREB3 5' Overlap uORF 5' Overlap uORF GN=CREB3 5' Overlap uORF AL20210827_EV_HLA_PDAC_PANFRO413_2D_50e6_W632_classI_fxn3 Exploris AL20221201_MKP_PDAC_01.29152.29284.2 2 2 1 11.78 6.42 5.46 5.36 84.8 5 1 1 535000000.0 Hydrogen 33 (G) 9 RWGPVAHPF (R) 9 LAELPTHFP R\W G P V\A|H|P/F 55.6 5 4 3 2 0 unbiased 0.952 100.0 47.42 66.41 0.0 533.7831 1066.558 0.0009 0.8 0.0 9.75 7318.4 nuORFdb_human ENST00000353704.2_2_9:35732632-35732818:+ ENST00000353704.2_2_9:35732632-35732818:+ CREB3 5' Overlap uORF 5' Overlap uORF GN=CREB3 5' Overlap uORF AL20221201_MKP_PDAC_01 SCP E_20221201_NO30_400nL_HLAc1_4e7_PDAC_Slot2-6_1_3524.13070.1396.2 2 2 1 14.27 8.35 7.37 5.92 92.3 6 1 1 398000.0 Hydrogen 33 (G) 9 RWGPVAHPF (R) 9 LPAAWQHPF R/W G\P V|A|H|P\F 55.6 5 5 4 3 0 dual b/y bias 45.154221 0.878962 533.7805 1066.558 -0.0034 -3.2 -0.8000000000000003 9.75 7318.4 nuORFdb_human ENST00000353704.2_2_9:35732632-35732818:+ ENST00000353704.2_2_9:35732632-35732818:+ CREB3 5' Overlap uORF 5' Overlap uORF GN=CREB3 5' Overlap uORF E_20221201_NO30_400nL_HLAc1_4e7_PDAC_Slot2-6_1_3524 Exploris_FAIMS VH20221207_MKP_FAIMS2022E_HLAc1_4e7_PDAC.52131.52131.2 2 4 1 10.17 6.14 4.3 4.03 78.5 5 1 1 375000000.0 Hydrogen 33 (G) 9 RWGPVAHPF (R) 9 LAELPTHFP R W G\P V\A\H|P/F 44.4 4 4 2 1 0 b-ion bias 0.997 68.3 91.38 19.4 -70.0 533.7815 1066.558 -0.0024 -2.2 0.3999999999999999 9.75 7318.4 nuORFdb_human ENST00000353704.2_2_9:35732632-35732818:+ ENST00000353704.2_2_9:35732632-35732818:+ CREB3 5' Overlap uORF 5' Overlap uORF GN=CREB3 5' Overlap uORF VH20221207_MKP_FAIMS2022E_HLAc1_4e7_PDAC Eva-50mio-3fr AL20210827_EV_HLA_PDAC_PANFRO413_2D_50e6_W632_classI_fxn3.55775.55887.2 2 1 1 13.39 6.28 6.29 7.11 83.3 7 1 1 16600000.0 Hydrogen 66 (E) 9 RWPPFLDLL (L) 9 DLLTNVQQLL R W|P\P\F\L\D|L|L 77.8 7 7 3 3 0 dual b/y bias 0.936 100.0 100.43 94.33 0.0 578.8304 1156.651 0.0022 1.9 1.4 5.84 10189.7 nuORFdb_human ENST00000571129.5_1_17:7144048-7144743:- ENST00000571129.5_1_17:7144048-7144743:- GABARAP 3' dORF 3' dORF GN=GABARAP 3' dORF AL20210827_EV_HLA_PDAC_PANFRO413_2D_50e6_W632_classI_fxn3 Exploris AL20221201_MKP_PDAC_01.68082.68082.2 2 1 1 16.18 8.8 9.02 7.37 92.9 7 1 1 43100000.0 Hydrogen 66 (E) 9 RWPPFLDLL (L) 9 KnRFHInLI R W|P\P\F\L\D|L|L 77.8 7 7 3 3 0 dual b/y bias 0.984 91.8 101.47 13.08 0.0 578.8303 1156.651 0.0019 1.7 0.8999999999999999 5.84 10189.7 nuORFdb_human ENST00000571129.5_1_17:7144048-7144743:- ENST00000571129.5_1_17:7144048-7144743:- GABARAP 3' dORF 3' dORF GN=GABARAP 3' dORF AL20221201_MKP_PDAC_01 SCP E_20221201_NO30_400nL_HLAc1_4e7_PDAC_Slot2-6_1_3524.30139.1400.2 2 1 1 11.48 8.4 2.04 3.08 82.5 6 1 1 8030.0 Hydrogen 66 (E) 9 RWPPFLDLL (L) 9 DRDIALLDLI R W\P\P F|L|D\L\L 55.6 5 6 2 2 0 b-ion bias 104.128464 0.876172 578.8279 1156.651 -0.0026 -2.3 0.10000000000000009 5.84 10189.7 nuORFdb_human ENST00000571129.5_1_17:7144048-7144743:- ENST00000571129.5_1_17:7144048-7144743:- GABARAP 3' dORF 3' dORF GN=GABARAP 3' dORF E_20221201_NO30_400nL_HLAc1_4e7_PDAC_Slot2-6_1_3524 Eva-50mio-3fr AL20210827_EV_HLA_PDAC_PANFRO413_2D_50e6_W632_classI_fxn3.29962.29962.3 3 1 1 11.35 0.05 8.25 11.3 70.5 7 2 2 7130000.0 Hydrogen 59 (A) 11 RWYPESPRNLW (K) 11 LLVnHTDYRWW R W Y\P/E|S/P R\N\L|W 54.5 6 5 4 2 0 unbiased 0.972 74.7 55.13 100.0 0.0 501.9215 1503.749 0.0009 0.6 0.09999999999999998 8.75 10155.8 nuORFdb_human ENST00000314616.10_1_17:26989278-27001327:+ ENST00000314616.10_1_17:26989278-27001327:+ SUPT6H Out-of-Frame Out-of-Frame GN=SUPT6H Out-of-Frame AL20210827_EV_HLA_PDAC_PANFRO413_2D_50e6_W632_classI_fxn3 Exploris_FAIMS VH20221207_MKP_FAIMS2022E_HLAc1_4e7_PDAC.60779.60779.3 3 3 1 15.62 4.29 10.54 11.33 83.6 8 2 2 32700000.0 Hydrogen 59 (A) 11 RWYPESPRNLW (K) 11 LAYIHSYSHLNW R W\Y\P/E|S|P R\N\L|W 63.6 7 7 4 3 0 unbiased 0.989 86.0 105.12 37.73 -70.0 501.92 1503.749 -0.0039 -2.6 0.0 8.75 10155.8 nuORFdb_human ENST00000314616.10_1_17:26989278-27001327:+ ENST00000314616.10_1_17:26989278-27001327:+ SUPT6H Out-of-Frame Out-of-Frame GN=SUPT6H Out-of-Frame VH20221207_MKP_FAIMS2022E_HLAc1_4e7_PDAC Eva-50mio-3fr AL20210827_EV_HLA_PDAC_PANFRO413_2D_50e6_W632_classI_fxn3.10811.10811.3 3 2 2 7.27 2.5 5.37 4.77 63.1 7 2 2 76200000.0 Hydrogen 2 (M) 9 RYIAQVKHL (-) 9 KARYLQVLH R Y\I\A\Q/V/K|H/L 77.8 7 4 4 1 0 unbiased 0.998 100.0 21.2 100.0 0.0 376.5611 1127.668 0.0003 0.3 -0.2 9.99 1258.5 nuORFdb_human ENST00000267068.5_2_13:33110791-33110824:- ENST00000267068.5_2_13:33110791-33110824:- N4BP2L2 Out-of-Frame Out-of-Frame GN=N4BP2L2 Out-of-Frame AL20210827_EV_HLA_PDAC_PANFRO413_2D_50e6_W632_classI_fxn3 Exploris AL20221201_MKP_PDAC_01.13270.13382.3 3 2 1 8.61 1.92 7.43 6.69 60.5 7 2 2 808000000.0 Hydrogen 2 (M) 9 RYIAQVKHL (-) 9 KARYLQVLH R Y\I|A\Q|V/K|H/L 77.8 7 5 5 3 0 dual b/y bias 1.0 76.2 24.55 100.0 0.0 376.5612 1127.668 0.0008 0.7 -0.10000000000000009 9.99 1258.5 nuORFdb_human ENST00000267068.5_2_13:33110791-33110824:- ENST00000267068.5_2_13:33110791-33110824:- N4BP2L2 Out-of-Frame Out-of-Frame GN=N4BP2L2 Out-of-Frame AL20221201_MKP_PDAC_01 SCP E_20221201_NO30_400nL_HLAc1_4e7_PDAC_Slot2-6_1_3524.5403.1617.3 3 3 1 15.62 9.82 9.32 5.8 76.5 7 2 2 358000.0 Hydrogen 2 (M) 9 RYIAQVKHL (-) 9 FTLAQAVKHI R Y\I|A|Q|V|K|H\L 77.8 7 7 5 5 0 dual b/y bias 18.673399 0.73945 376.559 1127.668 -0.0059 -5.3 -2.9 9.99 1258.5 nuORFdb_human ENST00000267068.5_2_13:33110791-33110824:- ENST00000267068.5_2_13:33110791-33110824:- N4BP2L2 Out-of-Frame Out-of-Frame GN=N4BP2L2 Out-of-Frame E_20221201_NO30_400nL_HLAc1_4e7_PDAC_Slot2-6_1_3524 Exploris_FAIMS VH20221207_MKP_FAIMS2022E_HLAc1_4e7_PDAC.17694.17794.3 3 2 1 9.27 3.18 9.76 6.1 60.8 8 2 2 101000000.0 Hydrogen 2 (M) 9 RYIAQVKHL (-) 9 KARYLQVLH R\Y\I\A\Q|V/K|H/L 100.0 9 6 4 2 0 unbiased 0.999 99.9 37.93 13.78 -50.0 376.5599 1127.668 -0.0034 -3.0 -0.3999999999999999 9.99 1258.5 nuORFdb_human ENST00000267068.5_2_13:33110791-33110824:- ENST00000267068.5_2_13:33110791-33110824:- N4BP2L2 Out-of-Frame Out-of-Frame GN=N4BP2L2 Out-of-Frame VH20221207_MKP_FAIMS2022E_HLAc1_4e7_PDAC Eva-50mio-3fr AL20210827_EV_HLA_PDAC_PANFRO413_2D_50e6_W632_classI_fxn3.26779.26779.3 3 1 1 11.56 1.29 7.01 10.28 65.6 8 2 2 4440000.0 Hydrogen 7 (S) 11 RYISQSGLKLL (I) 11 IKSAVLRYNIT R\Y\I\S Q|S/G L|K-L\L 63.6 7 6 3 2 1 b-ion bias 0.998 100.0 49.62 100.0 0.0 426.5903 1277.758 -0.0013 -1.0 -1.5 9.99 5023.7 nuORFdb_human ENST00000514616.5_1_5:137223855-137224842:- ENST00000514616.5_1_5:137223855-137224842:- RP11-381K20.2 Antisense lncRNA GN=RP11-381K20.2 Antisense AL20210827_EV_HLA_PDAC_PANFRO413_2D_50e6_W632_classI_fxn3 SCP E_20221201_NO30_400nL_HLAc1_4e7_PDAC_Slot2-6_1_3524.25697.1238.2 2 2 1 9.71 6.78 3.49 2.93 55.3 7 2 2 13000.0 Hydrogen 7 (S) 11 RYISQSGLKLL (I) 11 AKYGSLDVAKLL R Y I|S Q\S|G\L|K\L\L 54.5 6 7 3 3 0 b-ion bias 88.77459 0.977679 639.3821 1277.758 -0.0009 -0.7 1.7 9.99 5023.7 nuORFdb_human ENST00000514616.5_1_5:137223855-137224842:- ENST00000514616.5_1_5:137223855-137224842:- RP11-381K20.2 Antisense lncRNA GN=RP11-381K20.2 Antisense E_20221201_NO30_400nL_HLAc1_4e7_PDAC_Slot2-6_1_3524 Eva-50mio-3fr AL20210827_EV_HLA_PDAC_PANFRO413_2D_50e6_W632_classI_fxn3.16536.16536.3 3 1 1 15.68 7.7 9.33 7.98 82.7 7 1 1 10100000.0 Hydrogen 63 (S) 9 RYLAHLAHL (-) 9 EDLALVLAHL R Y\L\A|H|L|A|H/L 77.8 7 6 5 4 0 dual b/y bias 0.994 87.9 31.58 69.78 0.0 365.2133 1093.627 -0.001 -0.9 -1.4 8.76 7684.5 nuORFdb_human ENST00000504152.5_1_6:30856506-30856812:+ ENST00000504152.5_1_6:30856506-30856812:+ DDR1 3' Overlap dORF 3' Overlap dORF GN=DDR1 3' Overlap dORF AL20210827_EV_HLA_PDAC_PANFRO413_2D_50e6_W632_classI_fxn3 Eva-50mio-3fr AL20210827_EV_HLA_PDAC_PANFRO413_2D_50e6_W632_classI_fxn3.14228.14228.3 3 1 1 11.09 11.09 11.09 53.2 7 2 2 68300000.0 Hydrogen 38 (S) 9 RYLHGLQKF (G) 9 R Y\L\H\G\L\Q|K/F 77.8 7 6 2 1 0 b-ion bias 0.864 77.0 27.8 54.51 0.0 387.8895 1161.653 0.0011 0.9 0.4 9.99 9837.2 nuORFdb_human ENST00000261597.8_1_18:2571510-2573058:+ ENST00000261597.8_1_18:2571510-2573058:+ NDC80 5' Overlap uORF 5' Overlap uORF GN=NDC80 5' Overlap uORF AL20210827_EV_HLA_PDAC_PANFRO413_2D_50e6_W632_classI_fxn3 SCP E_20221201_NO30_400nL_HLAc1_4e7_PDAC_Slot2-6_1_3524.7679.1554.3 3 2 1 14.93 7.6 8.62 7.33 77.5 7 2 2 28800.0 Hydrogen 38 (S) 9 RYLHGLQKF (G) 9 IRYHGRGRF R Y\L|H|G|L\Q\K\F 77.8 7 7 3 3 0 dual b/y bias 26.531218 0.779155 387.8876 1161.653 -0.0033 -2.8 -0.3999999999999999 9.99 9837.2 nuORFdb_human ENST00000261597.8_1_18:2571510-2573058:+ ENST00000261597.8_1_18:2571510-2573058:+ NDC80 5' Overlap uORF 5' Overlap uORF GN=NDC80 5' Overlap uORF E_20221201_NO30_400nL_HLAc1_4e7_PDAC_Slot2-6_1_3524 Eva-50mio-3fr AL20210827_EV_HLA_PDAC_PANFRO413_2D_50e6_W632_classI_fxn3.18651.18651.3 3 1 1 21.23 5.33 13.4 15.9 91.6 9 2 2 1230000.0 Hydrogen 67 (R) 11 RYLPGCQHRLW (-) 11 qLYRHGTSLEIW R Y\L\P/G|C|Q|H/R\L/W 81.8 9 6 6 3 0 unbiased Carbamidomethylation C:Carbamidomethylation, 0.53 100.0 35.23 100.0 0.0 495.9227 1485.753 0.0004 0.3 -0.2 10.84 8634.7 nuORFdb_human ENST00000396832.5_2_22:38699234-38713288:- ENST00000396832.5_2_22:38699234-38713288:-|ENST00000359867.7_2_22:38699234-38714066:-|ENST00000400206.7_2_22:38699231-38732640:- CSNK1E 5' Overlap uORF 5' Overlap uORF GN=CSNK1E 5' Overlap uORF AL20210827_EV_HLA_PDAC_PANFRO413_2D_50e6_W632_classI_fxn3 Exploris AL20221201_MKP_PDAC_01.51095.51095.3 3 1 1 16.08 0.3 15.78 15.78 71.8 8 2 2 6830000.0 Hydrogen 67 (R) 11 RYLPGCQHRLW (-) 11 KNHGRCGPRYIAG R Y\L\P/G\C|Q|H/R\L W 63.6 7 6 4 2 0 unbiased Carbamidomethylation C:Carbamidomethylation, 0.799 100.0 78.0 100.0 0.0 495.9234 1485.753 0.0024 1.6 0.8 10.84 8634.7 nuORFdb_human ENST00000396832.5_2_22:38699234-38713288:- ENST00000396832.5_2_22:38699234-38713288:-|ENST00000359867.7_2_22:38699234-38714066:-|ENST00000400206.7_2_22:38699231-38732640:- CSNK1E 5' Overlap uORF 5' Overlap uORF GN=CSNK1E 5' Overlap uORF AL20221201_MKP_PDAC_01 SCP E_20221201_NO30_400nL_HLAc1_4e7_PDAC_Slot2-6_1_3524.27555.1371.2 2 1 1 9.6 7.88 2.28 1.72 71.2 5 2 2 14100.0 Hydrogen 54 (Y) 8 RYMKALFI (-) 8 ALGnRHIFL R Y M\K\A\L|F\I 62.5 5 5 1 1 0 b-ion bias 95.19754 0.894262 521.2988 1041.591 -0.0007 -0.6 1.7999999999999998 9.99 7427.7 smORF_v4|nuORFdb_human SLC45A4_8:142264367-142264552:- SLC45A4_8:142264367-142264552:-|ENST00000521804.1_1_8:142264369-142264606:- SLC45A4 uORF 8:142264367-142264552:- 8 SLC45A4 ncRNA Processed Transcript lncRNA GN=SLC45A4 E_20221201_NO30_400nL_HLAc1_4e7_PDAC_Slot2-6_1_3524 Exploris AL20221201_MKP_PDAC_01.22564.22564.2 2 2 1 15.43 1.85 15.43 13.58 74.9 6 1 1 58100000.0 Hydrogen 117 (S) 10 RYNHIVGCTF (S) 10 SAVLPAnSVTFC R Y N H\I\V\G\C|T/F 60.0 6 5 2 1 0 b-ion bias Carbamidomethylation C:Carbamidomethylation, 0.865 79.8 38.27 100.0 0.0 633.8059 1266.605 -0.0003 -0.2 -1.0 8.75 14759.1 nuORFdb_human ENST00000367607.7_2_1:179924016-179959652:+ ENST00000367607.7_2_1:179924016-179959652:+ CEP350 5' Overlap uORF 5' Overlap uORF GN=CEP350 5' Overlap uORF AL20221201_MKP_PDAC_01 SCP E_20221201_NO30_400nL_HLAc1_4e7_PDAC_Slot2-6_1_3524.10190.1290.2 2 2 1 18.86 6.07 13.09 12.79 86.5 7 1 1 17300.0 Hydrogen 117 (S) 10 RYNHIVGCTF (S) 10 RKNFFFCTF R Y N\H|I|V|G|C|T\F 70.0 7 7 5 5 0 dual b/y bias Carbamidomethylation C:Carbamidomethylation, 35.204552 0.945275 633.8046 1266.605 -0.0021 -1.6 0.7999999999999998 8.75 14759.1 nuORFdb_human ENST00000367607.7_2_1:179924016-179959652:+ ENST00000367607.7_2_1:179924016-179959652:+ CEP350 5' Overlap uORF 5' Overlap uORF GN=CEP350 5' Overlap uORF E_20221201_NO30_400nL_HLAc1_4e7_PDAC_Slot2-6_1_3524 Exploris_FAIMS VH20221207_MKP_FAIMS2022E_HLAc1_4e7_PDAC.28318.28318.2 2 1 1 19.24 5.06 10.98 14.18 91.4 7 1 1 2540000.0 Hydrogen 117 (S) 10 RYNHIVGCTF (S) 10 IHRYNEKCF R Y N\H\I\V\G\C|T/F 70.0 7 6 2 1 0 b-ion bias Carbamidomethylation C:Carbamidomethylation, 0.948 100.0 56.28 120.0 -50.0 633.8049 1266.605 -0.0022 -1.7 0.9000000000000001 8.75 14759.1 nuORFdb_human ENST00000367607.7_2_1:179924016-179959652:+ ENST00000367607.7_2_1:179924016-179959652:+ CEP350 5' Overlap uORF 5' Overlap uORF GN=CEP350 5' Overlap uORF VH20221207_MKP_FAIMS2022E_HLAc1_4e7_PDAC SCP E_20221201_NO30_400nL_HLAc1_4e7_PDAC_Slot2-6_1_3524.28612.1235.2 2 2 1 17.05 7.96 9.26 9.09 88.6 8 1 1 15500.0 Hydrogen 22 (L) 11 RYPPPLYWAEF (A) 11 LPVHPVEnSVAEF R Y|P\P\P L\Y|W|A|E\F 63.6 7 8 4 4 0 dual b/y bias 98.851585 0.979182 719.8598 1438.715 -0.0027 -1.9 0.5 6.0 8876.2 nuORFdb_human ENST00000634778.1_1_1:21502219-21502444:- ENST00000634778.1_1_1:21502219-21502444:- EIF4G3 ncRNA Processed Transcript lncRNA GN=EIF4G3 ncRNA Processed Transcript E_20221201_NO30_400nL_HLAc1_4e7_PDAC_Slot2-6_1_3524 Exploris_FAIMS VH20221207_MKP_FAIMS2022E_HLAc1_4e7_PDAC.62464.62464.2 2 1 1 12.54 8.58 3.96 3.96 67.6 6 2 2 8980000.0 Hydrogen 37 (S) 9 RYPVNLWRI (-) 9 RWFHIVFVL R|Y|P V N\L\W\R\I 66.7 6 6 2 2 0 b-ion bias 0.905 88.9 107.27 120.0 -50.0 608.8504 1216.695 -0.0014 -1.2 1.4000000000000001 10.84 5114.9 nuORFdb_human T265047_4:56806716-56814058:- T265047_4:56806716-56814058:-|T265048_4:56806716-56814024:- lincRNA lncRNA GN=nan lincRNA VH20221207_MKP_FAIMS2022E_HLAc1_4e7_PDAC SCP E_20221201_NO30_400nL_HLAc1_4e7_PDAC_Slot2-6_1_3524.7547.1559.3 3 1 1 8.3 4.27 5.36 4.03 70.8 6 2 2 4800.0 Hydrogen 9 (S) 11 RYTSVAAKSAF (-) 11 VPGGPAGAAAAYKA R Y T\S|V|A/A/K S A-F 45.5 5 3 4 2 1 unbiased 26.075588 0.776362 400.8819 1200.637 -0.0057 -4.7 -2.3000000000000003 9.99 2215.4 nuORFdb_human ENST00000215829.7_1_22:24963979-24964039:+ ENST00000215829.7_1_22:24963979-24964039:+ SNRPD3 Out-of-Frame Out-of-Frame GN=SNRPD3 Out-of-Frame E_20221201_NO30_400nL_HLAc1_4e7_PDAC_Slot2-6_1_3524 40mio-rep1 E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep1_Slot1-04_1_3496.8624.1364.2 2 1 1 12.56 7.23 4.9 5.33 76.7 9 1 2 8950.0 Hydrogen 2 (V) 10 SAAAVAKRVW (R) 10 SAAALGGSRIR S/A/A|A|V/A/K/R\V\W 100.0 10 4 7 2 0 unbiased 29.795183 0.898731 529.8077 1058.611 -0.0018 -1.7 1.5000000000000002 11.0 10302.6 nuORFdb_human ENST00000216520.6_1_14:69847257-69864987:- ENST00000216520.6_1_14:69847257-69864987:-|ENST00000555373.1_1_14:69861570-69864996:- ERH 5' Overlap uORF 5' Overlap uORF GN=ERH 5' Overlap uORF E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep1_Slot1-04_1_3496 SCP E_20221201_NO30_400nL_HLAc1_4e7_PDAC_Slot2-6_1_3524.12112.719.1 1 1 1 9.76 7.82 2.36 1.94 81.0 5 0 0 6140.0 Hydrogen 69 (A) 9 SAAPATPGL (R) 9 ASATAPGLP S A/A/P A|T|P-G L 33.3 3 2 4 2 1 y-ion bias 41.842888 1.294794 784.4194 784.42 -0.0009 -1.2 1.2 5.24 9515.7 nuORFdb_human ENST00000356166.10_3_16:30670552-30670825:+ ENST00000356166.10_3_16:30670552-30670825:+ FBRS 5' uORF 5' uORF GN=FBRS 5' uORF E_20221201_NO30_400nL_HLAc1_4e7_PDAC_Slot2-6_1_3524 10mio-rep1 E_20221201_NO30_400nL_HLAc1_1e7_directIP_titration_rep1_Slot2-3_1_3512.4669.1435.2 2 2 1 10.61 4.64 6.56 5.98 73.2 7 1 2 8750.0 Hydrogen 60 (A) 10 SAAPNAERKW (V) 10 GTAPGRAGSVWA S A/A/P/N/A E|R/K\W 60.0 6 2 6 1 0 y-ion bias 16.136217 0.854658 565.2883 1129.575 -0.0062 -5.5 2.2 8.46 14276.2 nuORFdb_human ENST00000366159.8_1_12:6442262-6443328:- ENST00000366159.8_1_12:6442262-6443328:- TNFRSF1A Out-of-Frame Out-of-Frame GN=TNFRSF1A Out-of-Frame E_20221201_NO30_400nL_HLAc1_1e7_directIP_titration_rep1_Slot2-3_1_3512 20mio-rep1 E_20221201_NO30_400nL_HLAc1_2e7_directIP_titration_rep1_Slot2-4_1_3515.4533.1648.3 3 3 1 10.54 5.21 4.33 5.34 90.7 8 1 2 33100.0 Hydrogen 60 (A) 10 SAAPNAERKW (V) 10 SAAPSYPSPPR S/A/A/P/N/A/E/R K\W 80.0 8 1 7 0 0 y-ion bias 15.665566 0.719835 377.1939 1129.575 -0.0074 -6.6 -0.39999999999999947 8.46 14276.2 nuORFdb_human ENST00000366159.8_1_12:6442262-6443328:- ENST00000366159.8_1_12:6442262-6443328:- TNFRSF1A Out-of-Frame Out-of-Frame GN=TNFRSF1A Out-of-Frame E_20221201_NO30_400nL_HLAc1_2e7_directIP_titration_rep1_Slot2-4_1_3515 20mio-rep2 E_20221201_NO30_400nL_HLAc1_2e7_directIP_titration_rep3_Slot2-4_1_3518.4624.1651.3 3 3 1 10.02 5.38 3.62 4.64 89.7 7 1 2 23800.0 Hydrogen 60 (A) 10 SAAPNAERKW (V) 10 SAAPSYPSPPR S/A/A/P/N/A/E/R K W 70.0 7 0 7 0 0 y-ion bias 15.983068 0.718113 377.193 1129.575 -0.0104 -9.2 -2.8999999999999995 8.46 14276.2 nuORFdb_human ENST00000366159.8_1_12:6442262-6443328:- ENST00000366159.8_1_12:6442262-6443328:- TNFRSF1A Out-of-Frame Out-of-Frame GN=TNFRSF1A Out-of-Frame E_20221201_NO30_400nL_HLAc1_2e7_directIP_titration_rep3_Slot2-4_1_3518 20mio-rep3 E_20221201_NO30_400nL_HLAc1_2e7_directIP_titration_rep4_Slot2-4_1_3519.4581.1647.3 3 3 1 9.79 7.56 2.55 2.23 90.4 6 1 2 38100.0 Hydrogen 60 (A) 10 SAAPNAERKW (V) 10 GTAPGRAGSVWA S/A/A/P/N/A E R K-W 60.0 6 0 5 0 1 y-ion bias 15.831577 0.720298 377.1935 1129.575 -0.0104 -9.2 -3.3999999999999995 8.46 14276.2 nuORFdb_human ENST00000366159.8_1_12:6442262-6443328:- ENST00000366159.8_1_12:6442262-6443328:- TNFRSF1A Out-of-Frame Out-of-Frame GN=TNFRSF1A Out-of-Frame E_20221201_NO30_400nL_HLAc1_2e7_directIP_titration_rep4_Slot2-4_1_3519 40mio-rep1 E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep1_Slot1-04_1_3496.4788.1624.3 3 3 1 9.76 5.69 3.58 4.08 89.5 7 1 2 58800.0 Hydrogen 60 (A) 10 SAAPNAERKW (V) 10 SAADIPINISE S/A/A/P/N/A/E R K-W 70.0 7 0 6 0 1 y-ion bias 16.548925 0.735275 377.1945 1129.575 -0.0074 -6.6 -3.3999999999999995 8.46 14276.2 nuORFdb_human ENST00000366159.8_1_12:6442262-6443328:- ENST00000366159.8_1_12:6442262-6443328:- TNFRSF1A Out-of-Frame Out-of-Frame GN=TNFRSF1A Out-of-Frame E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep1_Slot1-04_1_3496 40mio-rep2 E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep2_Slot2-5_1_3520.4545.1636.3 3 4 1 12.07 7.25 4.56 4.81 91.7 9 1 2 114000.0 Hydrogen 60 (A) 10 SAAPNAERKW (V) 10 SAAPSYPSPPR S/A/A/P/N/A/E/R-K-W 100.0 10 0 7 0 2 y-ion bias 15.709836 0.727769 377.1942 1129.575 -0.0074 -6.6 -1.6999999999999993 8.46 14276.2 nuORFdb_human ENST00000366159.8_1_12:6442262-6443328:- ENST00000366159.8_1_12:6442262-6443328:- TNFRSF1A Out-of-Frame Out-of-Frame GN=TNFRSF1A Out-of-Frame E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep2_Slot2-5_1_3520 40mio-rep3 E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep3_Slot2-5_1_3521.4558.1425.2 2 4 1 11.21 6.53 4.38 4.67 78.4 8 1 2 84600.0 Hydrogen 60 (A) 10 SAAPNAERKW (V) 10 SAAPQnLSLEV S/A/A|P/N/A-E|R K\W 80.0 8 3 6 2 1 y-ion bias 15.751509 0.860893 565.2874 1129.575 -0.0082 -7.2 -2.6000000000000005 8.46 14276.2 nuORFdb_human ENST00000366159.8_1_12:6442262-6443328:- ENST00000366159.8_1_12:6442262-6443328:- TNFRSF1A Out-of-Frame Out-of-Frame GN=TNFRSF1A Out-of-Frame E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep3_Slot2-5_1_3521 40mio-rep4 E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep4_Slot2-5_1_3522.4560.1625.3 3 7 1 12.85 8.15 4.73 4.7 89.8 7 1 2 176000.0 Hydrogen 60 (A) 10 SAAPNAERKW (V) 10 SAAPSYPSPPR S/A/A|P/N/A/E R K-W 70.0 7 1 6 1 1 y-ion bias 15.756331 0.734329 377.1945 1129.575 -0.0044 -3.9 0.8000000000000003 8.46 14276.2 nuORFdb_human ENST00000366159.8_1_12:6442262-6443328:- ENST00000366159.8_1_12:6442262-6443328:- TNFRSF1A Out-of-Frame Out-of-Frame GN=TNFRSF1A Out-of-Frame E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep4_Slot2-5_1_3522 5mio-rep1 E_20221201_NO30_400nL_HLAc1_5e6_directIP_titration_rep1_Slot1-37_1_3502.4805.1424.2 2 1 1 10.36 6.01 5.39 4.35 74.4 7 1 2 3020.0 Hydrogen 60 (A) 10 SAAPNAERKW (V) 10 SAAPGTRTGSGPA S/A/A/P/N|A/E/R K W 70.0 7 1 7 1 0 y-ion bias 16.604748 0.861577 565.2864 1129.575 -0.0102 -9.0 -2.0 8.46 14276.2 nuORFdb_human ENST00000366159.8_1_12:6442262-6443328:- ENST00000366159.8_1_12:6442262-6443328:- TNFRSF1A Out-of-Frame Out-of-Frame GN=TNFRSF1A Out-of-Frame E_20221201_NO30_400nL_HLAc1_5e6_directIP_titration_rep1_Slot1-37_1_3502 10mio-rep1 E_20221201_NO30_400nL_HLAc1_1e7_directIP_titration_rep1_Slot2-3_1_3512.8360.1611.2 2 5 1 15.84 9.22 5.27 6.62 93.6 7 1 1 20300.0 Hydrogen 22 (A) 8 SAAVRVAY (R) 8 SASPIKAY S/A/A|V/R|V|A\Y 100.0 8 4 6 3 0 dual b/y bias 28.886044 0.743096 418.7312 836.462 -0.0078 -9.3 -1.6000000000000005 8.46 3260.6 nuORFdb_human ENST00000427191.6_1_4:87515508-87515601:+ ENST00000427191.6_1_4:87515508-87515601:+ PTPN13 5' uORF 5' uORF GN=PTPN13 5' uORF E_20221201_NO30_400nL_HLAc1_1e7_directIP_titration_rep1_Slot2-3_1_3512 10mio-rep2 E_20221201_NO30_400nL_HLAc1_1e7_directIP_titration_rep2_Slot2-3_1_3513.8310.1601.2 2 3 1 13.18 8.42 4.95 4.75 88.9 7 1 1 20200.0 Hydrogen 22 (A) 8 SAAVRVAY (R) 8 TGAVALAAY S/A/A|V/R/V|A\Y 100.0 8 3 6 2 0 y-ion bias 28.712412 0.749557 418.7312 836.462 -0.0078 -9.3 -2.6000000000000005 8.46 3260.6 nuORFdb_human ENST00000427191.6_1_4:87515508-87515601:+ ENST00000427191.6_1_4:87515508-87515601:+ PTPN13 5' uORF 5' uORF GN=PTPN13 5' uORF E_20221201_NO30_400nL_HLAc1_1e7_directIP_titration_rep2_Slot2-3_1_3513 10mio-rep3 E_20221201_NO30_400nL_HLAc1_1e7_directIP_titration_rep3_Slot2-3_1_3514.8317.1605.2 2 3 1 13.17 8.64 3.53 4.53 86.7 7 1 1 16100.0 Hydrogen 22 (A) 8 SAAVRVAY (R) 8 GTRLGVAY S/A/A/V/R|V|A\Y 100.0 8 3 6 2 0 y-ion bias 28.738739 0.747181 418.7319 836.462 -0.0058 -6.9 -0.6000000000000005 8.46 3260.6 nuORFdb_human ENST00000427191.6_1_4:87515508-87515601:+ ENST00000427191.6_1_4:87515508-87515601:+ PTPN13 5' uORF 5' uORF GN=PTPN13 5' uORF E_20221201_NO30_400nL_HLAc1_1e7_directIP_titration_rep3_Slot2-3_1_3514 20mio-rep1 E_20221201_NO30_400nL_HLAc1_2e7_directIP_titration_rep1_Slot2-4_1_3515.8242.1610.2 2 2 1 13.29 9.61 4.59 3.68 91.8 7 1 1 14800.0 Hydrogen 22 (A) 8 SAAVRVAY (R) 8 TGAVALAAY S/A/A|V/R\V|A\Y 100.0 8 4 5 2 0 unbiased 28.472168 0.743662 418.7326 836.462 -0.0038 -4.5 1.7000000000000002 8.46 3260.6 nuORFdb_human ENST00000427191.6_1_4:87515508-87515601:+ ENST00000427191.6_1_4:87515508-87515601:+ PTPN13 5' uORF 5' uORF GN=PTPN13 5' uORF E_20221201_NO30_400nL_HLAc1_2e7_directIP_titration_rep1_Slot2-4_1_3515 20mio-rep2 E_20221201_NO30_400nL_HLAc1_2e7_directIP_titration_rep3_Slot2-4_1_3518.8399.1609.2 2 1 1 13.29 8.23 4.22 5.05 91.5 7 1 1 9300.0 Hydrogen 22 (A) 8 SAAVRVAY (R) 8 GTRLGVAY S/A/A/V/R\V\A\Y 100.0 8 3 4 0 0 unbiased 29.015099 0.744412 418.7322 836.462 -0.0058 -6.9 -0.6000000000000005 8.46 3260.6 nuORFdb_human ENST00000427191.6_1_4:87515508-87515601:+ ENST00000427191.6_1_4:87515508-87515601:+ PTPN13 5' uORF 5' uORF GN=PTPN13 5' uORF E_20221201_NO30_400nL_HLAc1_2e7_directIP_titration_rep3_Slot2-4_1_3518 20mio-rep3 E_20221201_NO30_400nL_HLAc1_2e7_directIP_titration_rep4_Slot2-4_1_3519.8281.1606.2 2 2 1 14.9 8.77 5.29 6.13 96.0 7 1 1 20900.0 Hydrogen 22 (A) 8 SAAVRVAY (R) 8 GTRLGVAY S/A/A|V/R\V|A\Y 100.0 8 4 5 2 0 unbiased 28.607885 0.74631 418.7329 836.462 -0.0038 -4.5 1.2999999999999998 8.46 3260.6 nuORFdb_human ENST00000427191.6_1_4:87515508-87515601:+ ENST00000427191.6_1_4:87515508-87515601:+ PTPN13 5' uORF 5' uORF GN=PTPN13 5' uORF E_20221201_NO30_400nL_HLAc1_2e7_directIP_titration_rep4_Slot2-4_1_3519 40mio-rep1 E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep1_Slot1-04_1_3496.8529.1578.2 2 1 1 13.5 8.21 4.38 5.29 93.4 7 1 1 28700.0 Hydrogen 22 (A) 8 SAAVRVAY (R) 8 GTRLGVAY S/A/A/V/R\V|A\Y 100.0 8 3 5 1 0 unbiased 29.467295 0.76438 418.7327 836.462 -0.0038 -4.5 -1.2999999999999998 8.46 3260.6 nuORFdb_human ENST00000427191.6_1_4:87515508-87515601:+ ENST00000427191.6_1_4:87515508-87515601:+ PTPN13 5' uORF 5' uORF GN=PTPN13 5' uORF E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep1_Slot1-04_1_3496 40mio-rep2 E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep2_Slot2-5_1_3520.8252.1596.2 2 1 1 12.78 7.77 4.99 5.01 91.8 7 1 1 26300.0 Hydrogen 22 (A) 8 SAAVRVAY (R) 8 TGLGRAVY S/A/A|V/R\V\A\Y 100.0 8 4 4 1 0 unbiased 28.510584 0.752573 418.7323 836.462 -0.0058 -6.9 -2.0 8.46 3260.6 nuORFdb_human ENST00000427191.6_1_4:87515508-87515601:+ ENST00000427191.6_1_4:87515508-87515601:+ PTPN13 5' uORF 5' uORF GN=PTPN13 5' uORF E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep2_Slot2-5_1_3520 40mio-rep3 E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep3_Slot2-5_1_3521.8263.1598.2 2 2 1 13.92 7.81 4.85 6.11 93.5 7 1 1 37700.0 Hydrogen 22 (A) 8 SAAVRVAY (R) 8 TGAVALAAY S/A/A|V/R\V|A\Y 100.0 8 4 5 2 0 unbiased 28.54505 0.751762 418.7314 836.462 -0.0078 -9.3 -4.700000000000001 8.46 3260.6 nuORFdb_human ENST00000427191.6_1_4:87515508-87515601:+ ENST00000427191.6_1_4:87515508-87515601:+ PTPN13 5' uORF 5' uORF GN=PTPN13 5' uORF E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep3_Slot2-5_1_3521 40mio-rep4 E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep4_Slot2-5_1_3522.8275.1595.2 2 2 1 13.92 8.21 4.8 5.71 93.2 7 1 1 43500.0 Hydrogen 22 (A) 8 SAAVRVAY (R) 8 GTRLGVAY S/A/A|V/R|V\A\Y 100.0 8 4 5 2 0 unbiased 28.582157 0.7533 418.7325 836.462 -0.0038 -4.5 0.20000000000000018 8.46 3260.6 nuORFdb_human ENST00000427191.6_1_4:87515508-87515601:+ ENST00000427191.6_1_4:87515508-87515601:+ PTPN13 5' uORF 5' uORF GN=PTPN13 5' uORF E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep4_Slot2-5_1_3522 5mio-rep1 E_20221201_NO30_400nL_HLAc1_5e6_directIP_titration_rep1_Slot1-37_1_3502.8576.1599.2 2 1 1 8.21 4.47 2.4 3.74 79.9 6 1 1 4360.0 Hydrogen 22 (A) 8 SAAVRVAY (R) 8 ASAQLLAY S/A/A/V/R V|A\Y 75.0 6 2 5 1 0 y-ion bias 29.626919 0.750813 418.7325 836.462 -0.0038 -4.5 2.5 8.46 3260.6 nuORFdb_human ENST00000427191.6_1_4:87515508-87515601:+ ENST00000427191.6_1_4:87515508-87515601:+ PTPN13 5' uORF 5' uORF GN=PTPN13 5' uORF E_20221201_NO30_400nL_HLAc1_5e6_directIP_titration_rep1_Slot1-37_1_3502 5mio-rep3 E_20221201_NO30_400nL_HLAc1_5e6_directIP_titration_rep3_Slot2-2_1_3511.8548.1609.2 2 1 1 8.44 6.87 2.91 1.56 79.0 6 1 1 2520.0 Hydrogen 22 (A) 8 SAAVRVAY (R) 8 TGAVALAAY S/A/A|V R\V\A\Y 75.0 6 4 3 1 0 unbiased 29.532213 0.744377 418.7298 836.462 -0.0098 -11.7 -3.5 8.46 3260.6 nuORFdb_human ENST00000427191.6_1_4:87515508-87515601:+ ENST00000427191.6_1_4:87515508-87515601:+ PTPN13 5' uORF 5' uORF GN=PTPN13 5' uORF E_20221201_NO30_400nL_HLAc1_5e6_directIP_titration_rep3_Slot2-2_1_3511 10mio-rep1 E_20221201_NO30_400nL_HLAc1_1e7_directIP_titration_rep1_Slot2-3_1_3512.16305.1489.2 2 5 1 11.99 7.72 3.8 4.27 87.6 7 0 1 33100.0 Hydrogen 19 (G) 9 SAAWDRPPL (R) 9 SAALPSCLPP S/A/A|W|D/R|P-P L 77.8 7 3 6 3 1 dual b/y bias 56.325207 0.820179 506.7588 1012.521 -0.0103 -10.2 -2.499999999999999 5.55 7371.0 nuORFdb_human ENST00000356432.7_1_16:129505-133057:+ ENST00000356432.7_1_16:129505-133057:+ MPG Out-of-Frame Out-of-Frame GN=MPG Out-of-Frame E_20221201_NO30_400nL_HLAc1_1e7_directIP_titration_rep1_Slot2-3_1_3512 10mio-rep2 E_20221201_NO30_400nL_HLAc1_1e7_directIP_titration_rep2_Slot2-3_1_3513.16250.1497.2 2 7 1 12.35 5.52 6.79 6.83 88.1 6 0 1 31100.0 Hydrogen 19 (G) 9 SAAWDRPPL (R) 9 GTAWHVWR S/A/A/W|D|R|P P L 66.7 6 3 6 3 0 dual b/y bias 56.134781 0.8156 506.7606 1012.521 -0.0063 -6.3 0.40000000000000036 5.55 7371.0 nuORFdb_human ENST00000356432.7_1_16:129505-133057:+ ENST00000356432.7_1_16:129505-133057:+ MPG Out-of-Frame Out-of-Frame GN=MPG Out-of-Frame E_20221201_NO30_400nL_HLAc1_1e7_directIP_titration_rep2_Slot2-3_1_3513 10mio-rep3 E_20221201_NO30_400nL_HLAc1_1e7_directIP_titration_rep3_Slot2-3_1_3514.16219.1492.2 2 5 1 13.03 6.41 7.83 6.62 89.2 6 0 1 35100.0 Hydrogen 19 (G) 9 SAAWDRPPL (R) 9 GTAWHVWR S A/A|W|D|R|P P\L 55.6 5 5 5 4 0 dual b/y bias 56.027893 0.818392 506.7615 1012.521 -0.0043 -4.3 2.0 5.55 7371.0 nuORFdb_human ENST00000356432.7_1_16:129505-133057:+ ENST00000356432.7_1_16:129505-133057:+ MPG Out-of-Frame Out-of-Frame GN=MPG Out-of-Frame E_20221201_NO30_400nL_HLAc1_1e7_directIP_titration_rep3_Slot2-3_1_3514 20mio-rep1 E_20221201_NO30_400nL_HLAc1_2e7_directIP_titration_rep1_Slot2-4_1_3515.16161.1491.2 2 7 1 14.25 6.59 7.1 7.66 90.6 7 0 1 84100.0 Hydrogen 19 (G) 9 SAAWDRPPL (R) 9 GTAWPSLPSP S/A/A/W|D|R|P P\L 77.8 7 4 6 3 0 dual b/y bias 55.814346 0.819217 506.7607 1012.521 -0.0063 -6.3 -0.09999999999999964 5.55 7371.0 nuORFdb_human ENST00000356432.7_1_16:129505-133057:+ ENST00000356432.7_1_16:129505-133057:+ MPG Out-of-Frame Out-of-Frame GN=MPG Out-of-Frame E_20221201_NO30_400nL_HLAc1_2e7_directIP_titration_rep1_Slot2-4_1_3515 20mio-rep2 E_20221201_NO30_400nL_HLAc1_2e7_directIP_titration_rep3_Slot2-4_1_3518.16282.1486.2 2 7 1 13.48 6.37 9.51 7.11 89.7 7 0 1 74800.0 Hydrogen 19 (G) 9 SAAWDRPPL (R) 9 GTAnRWPIP S/A/A/W|D|R|P\P L 77.8 7 4 6 3 0 dual b/y bias 56.237572 0.822612 506.7606 1012.521 -0.0063 -6.3 0.0 5.55 7371.0 nuORFdb_human ENST00000356432.7_1_16:129505-133057:+ ENST00000356432.7_1_16:129505-133057:+ MPG Out-of-Frame Out-of-Frame GN=MPG Out-of-Frame E_20221201_NO30_400nL_HLAc1_2e7_directIP_titration_rep3_Slot2-4_1_3518 20mio-rep3 E_20221201_NO30_400nL_HLAc1_2e7_directIP_titration_rep4_Slot2-4_1_3519.16293.1483.2 2 6 1 14.33 6.69 7.57 7.65 85.2 8 0 1 72400.0 Hydrogen 19 (G) 9 SAAWDRPPL (R) 9 GTAWHVWR S/A/A/W|D|R|P\P\L 100.0 9 5 6 3 0 dual b/y bias 56.276527 0.824261 506.7606 1012.521 -0.0063 -6.3 -0.5 5.55 7371.0 nuORFdb_human ENST00000356432.7_1_16:129505-133057:+ ENST00000356432.7_1_16:129505-133057:+ MPG Out-of-Frame Out-of-Frame GN=MPG Out-of-Frame E_20221201_NO30_400nL_HLAc1_2e7_directIP_titration_rep4_Slot2-4_1_3519 40mio-rep1 E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep1_Slot1-04_1_3496.16436.1462.2 2 6 1 17.4 7.76 7.82 9.63 95.2 8 0 1 199000.0 Hydrogen 19 (G) 9 SAAWDRPPL (R) 9 GTAWPSLPSP S/A/A|W|D|R|P\P\L 100.0 9 6 6 4 0 dual b/y bias 56.775078 0.837386 506.7622 1012.521 -0.0043 -4.3 -1.0999999999999996 5.55 7371.0 nuORFdb_human ENST00000356432.7_1_16:129505-133057:+ ENST00000356432.7_1_16:129505-133057:+ MPG Out-of-Frame Out-of-Frame GN=MPG Out-of-Frame E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep1_Slot1-04_1_3496 40mio-rep2 E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep2_Slot2-5_1_3520.16117.1477.2 2 6 1 18.58 9.98 9.12 8.6 96.2 8 0 1 139000.0 Hydrogen 19 (G) 9 SAAWDRPPL (R) 9 GTAnRWPIP S/A/A|W|D|R|P|P\L 100.0 9 6 7 5 0 dual b/y bias 55.673779 0.82816 506.7619 1012.521 -0.0043 -4.3 0.6000000000000005 5.55 7371.0 nuORFdb_human ENST00000356432.7_1_16:129505-133057:+ ENST00000356432.7_1_16:129505-133057:+ MPG Out-of-Frame Out-of-Frame GN=MPG Out-of-Frame E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep2_Slot2-5_1_3520 40mio-rep3 E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep3_Slot2-5_1_3521.16150.1481.2 2 6 1 16.47 7.54 10.11 8.93 93.7 8 0 1 106000.0 Hydrogen 19 (G) 9 SAAWDRPPL (R) 9 SAALPSCLPP S/A/A|W|D|R|P/P\L 100.0 9 5 7 4 0 dual b/y bias 55.789558 0.825401 506.762 1012.521 -0.0043 -4.3 0.2999999999999998 5.55 7371.0 nuORFdb_human ENST00000356432.7_1_16:129505-133057:+ ENST00000356432.7_1_16:129505-133057:+ MPG Out-of-Frame Out-of-Frame GN=MPG Out-of-Frame E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep3_Slot2-5_1_3521 40mio-rep4 E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep4_Slot2-5_1_3522.16128.1477.2 2 6 1 15.14 7.54 7.03 7.6 91.7 8 0 1 111000.0 Hydrogen 19 (G) 9 SAAWDRPPL (R) 9 SAALPSCLPP S/A/A|W|D|R|P-P\L 100.0 9 5 6 4 1 dual b/y bias 55.697937 0.827747 506.7606 1012.521 -0.0063 -6.3 -1.5999999999999996 5.55 7371.0 nuORFdb_human ENST00000356432.7_1_16:129505-133057:+ ENST00000356432.7_1_16:129505-133057:+ MPG Out-of-Frame Out-of-Frame GN=MPG Out-of-Frame E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep4_Slot2-5_1_3522 5mio-rep1 E_20221201_NO30_400nL_HLAc1_5e6_directIP_titration_rep1_Slot1-37_1_3502.16506.1481.2 2 12 1 14.02 6.06 6.38 7.96 87.8 8 0 1 43500.0 Hydrogen 19 (G) 9 SAAWDRPPL (R) 9 GTAWPSLPSP S/A/A/W|D/R|P/P\L 100.0 9 3 7 2 0 y-ion bias 57.016182 0.825576 506.7613 1012.521 -0.0063 -6.3 0.7000000000000002 5.55 7371.0 nuORFdb_human ENST00000356432.7_1_16:129505-133057:+ ENST00000356432.7_1_16:129505-133057:+ MPG Out-of-Frame Out-of-Frame GN=MPG Out-of-Frame E_20221201_NO30_400nL_HLAc1_5e6_directIP_titration_rep1_Slot1-37_1_3502 5mio-rep2 E_20221201_NO30_400nL_HLAc1_5e6_directIP_titration_rep2_Slot2-2_1_3510.16388.1513.2 2 2 1 10.53 4.98 4.45 5.55 81.8 7 0 1 6180.0 Hydrogen 19 (G) 9 SAAWDRPPL (R) 9 GTAWPSLPSP S/A/A/W|D/R|P\P L 77.8 7 3 6 2 0 y-ion bias 56.603237 0.805195 506.7611 1012.521 -0.0063 -6.3 2.3999999999999995 5.55 7371.0 nuORFdb_human ENST00000356432.7_1_16:129505-133057:+ ENST00000356432.7_1_16:129505-133057:+ MPG Out-of-Frame Out-of-Frame GN=MPG Out-of-Frame E_20221201_NO30_400nL_HLAc1_5e6_directIP_titration_rep2_Slot2-2_1_3510 5mio-rep3 E_20221201_NO30_400nL_HLAc1_5e6_directIP_titration_rep3_Slot2-2_1_3511.16402.1486.2 2 5 1 9.73 3.8 6.53 5.93 77.0 6 0 1 13700.0 Hydrogen 19 (G) 9 SAAWDRPPL (R) 9 SAQEDRLPP S A/A/W|D/R|P P\L 55.6 5 3 5 2 0 unbiased 56.653992 0.822257 506.7589 1012.521 -0.0103 -10.2 -2.0 5.55 7371.0 nuORFdb_human ENST00000356432.7_1_16:129505-133057:+ ENST00000356432.7_1_16:129505-133057:+ MPG Out-of-Frame Out-of-Frame GN=MPG Out-of-Frame E_20221201_NO30_400nL_HLAc1_5e6_directIP_titration_rep3_Slot2-2_1_3511 1mio-rep1 E_20221201_NO30_400nL_HLAc1_1e6_directIP_titration_rep1_Slot1-06_1_3498.8955.1534.2 2 4 1 13.78 8.71 7.54 5.08 91.5 8 1 1 11300.0 Hydrogen 42 (A) 9 SADPGNLKY (S) 9 SEGPGNIPPP S/A/D|P/G/N/L|K\Y 100.0 9 3 7 2 0 y-ion bias 30.936127 0.792066 482.7383 964.473 -0.0047 -4.9 0.0 5.55 48244.4 nuORFdb_human ENST00000522480.5_1_16:21481075-21513207:- ENST00000522480.5_1_16:21481075-21513207:-|ENST00000522841.6_1_16:21481075-21531198:- SMG1P3 Pseudogene Pseudogene GN=SMG1P3 Pseudogene E_20221201_NO30_400nL_HLAc1_1e6_directIP_titration_rep1_Slot1-06_1_3498 10mio-rep1 E_20221201_NO30_400nL_HLAc1_1e7_directIP_titration_rep1_Slot2-3_1_3512.8629.1544.2 2 4 1 15.71 10.25 7.68 5.46 90.6 8 1 1 36500.0 Hydrogen 42 (A) 9 SADPGNLKY (S) 9 SEGPGNIPPP S/A/D|P/G/N|L|K\Y 100.0 9 4 7 3 0 dual b/y bias 29.81448 0.785729 482.7365 964.473 -0.0087 -9.0 -1.2999999999999998 5.55 48244.4 nuORFdb_human ENST00000522480.5_1_16:21481075-21513207:- ENST00000522480.5_1_16:21481075-21513207:-|ENST00000522841.6_1_16:21481075-21531198:- SMG1P3 Pseudogene Pseudogene GN=SMG1P3 Pseudogene E_20221201_NO30_400nL_HLAc1_1e7_directIP_titration_rep1_Slot2-3_1_3512 10mio-rep2 E_20221201_NO30_400nL_HLAc1_1e7_directIP_titration_rep2_Slot2-3_1_3513.8598.1574.2 2 3 1 16.47 10.41 8.7 6.06 90.9 8 1 1 20500.0 Hydrogen 42 (A) 9 SADPGNLKY (S) 9 SEGPGNIPPP S/A/D|P|G\N|L\K\Y 100.0 9 6 5 3 0 dual b/y bias 29.707333 0.766998 482.7378 964.473 -0.0047 -4.9 1.7999999999999998 5.55 48244.4 nuORFdb_human ENST00000522480.5_1_16:21481075-21513207:- ENST00000522480.5_1_16:21481075-21513207:-|ENST00000522841.6_1_16:21481075-21531198:- SMG1P3 Pseudogene Pseudogene GN=SMG1P3 Pseudogene E_20221201_NO30_400nL_HLAc1_1e7_directIP_titration_rep2_Slot2-3_1_3513 10mio-rep3 E_20221201_NO30_400nL_HLAc1_1e7_directIP_titration_rep3_Slot2-3_1_3514.8592.1546.2 2 4 1 15.36 8.65 7.52 6.71 92.0 7 1 1 31200.0 Hydrogen 42 (A) 9 SADPGNLKY (S) 9 SEGPGNIPPP S A/D|P|G/N|L|K\Y 77.8 7 5 6 4 0 dual b/y bias 29.68878 0.784549 482.7377 964.473 -0.0047 -4.9 1.3999999999999995 5.55 48244.4 nuORFdb_human ENST00000522480.5_1_16:21481075-21513207:- ENST00000522480.5_1_16:21481075-21513207:-|ENST00000522841.6_1_16:21481075-21531198:- SMG1P3 Pseudogene Pseudogene GN=SMG1P3 Pseudogene E_20221201_NO30_400nL_HLAc1_1e7_directIP_titration_rep3_Slot2-3_1_3514 20mio-rep1 E_20221201_NO30_400nL_HLAc1_2e7_directIP_titration_rep1_Slot2-4_1_3515.8524.1541.2 2 3 1 16.65 9.44 9.64 7.21 93.6 7 1 1 52300.0 Hydrogen 42 (A) 9 SADPGNLKY (S) 9 SEGPGNIPPP S/A/D|P/G N|L|K\Y 77.8 7 4 6 3 0 dual b/y bias 29.445467 0.78734 482.7377 964.473 -0.0047 -4.9 1.2999999999999998 5.55 48244.4 nuORFdb_human ENST00000522480.5_1_16:21481075-21513207:- ENST00000522480.5_1_16:21481075-21513207:-|ENST00000522841.6_1_16:21481075-21531198:- SMG1P3 Pseudogene Pseudogene GN=SMG1P3 Pseudogene E_20221201_NO30_400nL_HLAc1_2e7_directIP_titration_rep1_Slot2-4_1_3515 20mio-rep2 E_20221201_NO30_400nL_HLAc1_2e7_directIP_titration_rep3_Slot2-4_1_3518.8680.1546.2 2 3 1 17.3 11.15 9.99 6.15 93.3 8 1 1 22000.0 Hydrogen 42 (A) 9 SADPGNLKY (S) 9 SEGPGNIPPP S/A/D|P|G/N|L|K\Y 100.0 9 5 7 4 0 dual b/y bias 29.98576 0.78456 482.7362 964.473 -0.0087 -9.0 -2.7 5.55 48244.4 nuORFdb_human ENST00000522480.5_1_16:21481075-21513207:- ENST00000522480.5_1_16:21481075-21513207:-|ENST00000522841.6_1_16:21481075-21531198:- SMG1P3 Pseudogene Pseudogene GN=SMG1P3 Pseudogene E_20221201_NO30_400nL_HLAc1_2e7_directIP_titration_rep3_Slot2-4_1_3518 20mio-rep3 E_20221201_NO30_400nL_HLAc1_2e7_directIP_titration_rep4_Slot2-4_1_3519.8571.1536.2 2 4 1 18.93 11.65 11.43 7.29 95.3 8 1 1 92900.0 Hydrogen 42 (A) 9 SADPGNLKY (S) 9 SEGPGNIPPP S/A/D|P|G/N|L|K\Y 100.0 9 5 7 4 0 dual b/y bias 29.608801 0.79106 482.7372 964.473 -0.0067 -7.0 -1.2000000000000002 5.55 48244.4 nuORFdb_human ENST00000522480.5_1_16:21481075-21513207:- ENST00000522480.5_1_16:21481075-21513207:-|ENST00000522841.6_1_16:21481075-21531198:- SMG1P3 Pseudogene Pseudogene GN=SMG1P3 Pseudogene E_20221201_NO30_400nL_HLAc1_2e7_directIP_titration_rep4_Slot2-4_1_3519 SCP E_20221201_NO30_400nL_HLAc1_4e7_PDAC_Slot2-6_1_3524.8536.1524.2 2 1 1 12.04 6.46 5.97 5.58 85.9 6 1 1 8400.0 Hydrogen 42 (A) 9 SADPGNLKY (S) 9 SEGPGNIPPP S/A/D|P/G N L|K\Y 66.7 6 3 5 2 0 unbiased 29.49008 0.798131 482.7381 964.473 -0.0047 -4.9 -2.5000000000000004 5.55 48244.4 nuORFdb_human ENST00000522480.5_1_16:21481075-21513207:- ENST00000522480.5_1_16:21481075-21513207:-|ENST00000522841.6_1_16:21481075-21531198:- SMG1P3 Pseudogene Pseudogene GN=SMG1P3 Pseudogene E_20221201_NO30_400nL_HLAc1_4e7_PDAC_Slot2-6_1_3524 40mio-rep1 E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep1_Slot1-04_1_3496.8825.1515.2 2 6 1 20.16 12.6 11.37 7.56 96.9 8 1 1 285000.0 Hydrogen 42 (A) 9 SADPGNLKY (S) 9 SEGPGNIPPP S/A/D|P|G/N|L|K\Y 100.0 9 5 7 4 0 dual b/y bias 30.489285 0.80401 482.7386 964.473 -0.0027 -2.8 0.40000000000000036 5.55 48244.4 nuORFdb_human ENST00000522480.5_1_16:21481075-21513207:- ENST00000522480.5_1_16:21481075-21513207:-|ENST00000522841.6_1_16:21481075-21531198:- SMG1P3 Pseudogene Pseudogene GN=SMG1P3 Pseudogene E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep1_Slot1-04_1_3496 40mio-rep2 E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep2_Slot2-5_1_3520.8527.1517.2 2 2 1 17.58 11.77 9.8 5.82 94.6 8 1 1 44400.0 Hydrogen 42 (A) 9 SADPGNLKY (S) 9 SEGPGNIPPP S/A/D|P/G/N|L|K\Y 100.0 9 4 7 3 0 dual b/y bias 29.459705 0.802992 482.7368 964.473 -0.0067 -7.0 -2.0999999999999996 5.55 48244.4 nuORFdb_human ENST00000522480.5_1_16:21481075-21513207:- ENST00000522480.5_1_16:21481075-21513207:-|ENST00000522841.6_1_16:21481075-21531198:- SMG1P3 Pseudogene Pseudogene GN=SMG1P3 Pseudogene E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep2_Slot2-5_1_3520 40mio-rep3 E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep3_Slot2-5_1_3521.8561.1521.2 2 4 1 18.85 11.26 9.11 7.59 95.1 8 1 1 190000.0 Hydrogen 42 (A) 9 SADPGNLKY (S) 9 SEGPGNIPPP S/A/D|P/G/N|L|K\Y 100.0 9 4 7 3 0 dual b/y bias 29.575907 0.800135 482.7376 964.473 -0.0047 -4.9 -0.3000000000000007 5.55 48244.4 nuORFdb_human ENST00000522480.5_1_16:21481075-21513207:- ENST00000522480.5_1_16:21481075-21513207:-|ENST00000522841.6_1_16:21481075-21531198:- SMG1P3 Pseudogene Pseudogene GN=SMG1P3 Pseudogene E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep3_Slot2-5_1_3521 40mio-rep4 E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep4_Slot2-5_1_3522.8538.1515.2 2 3 1 18.29 10.23 9.47 8.06 94.8 8 1 1 52200.0 Hydrogen 42 (A) 9 SADPGNLKY (S) 9 SEGPGNIPPP S/A/D|P|G/N|L\K\Y 100.0 9 5 6 3 0 dual b/y bias 29.489878 0.803813 482.7376 964.473 -0.0047 -4.9 -0.20000000000000018 5.55 48244.4 nuORFdb_human ENST00000522480.5_1_16:21481075-21513207:- ENST00000522480.5_1_16:21481075-21513207:-|ENST00000522841.6_1_16:21481075-21531198:- SMG1P3 Pseudogene Pseudogene GN=SMG1P3 Pseudogene E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep4_Slot2-5_1_3522 5mio-rep1 E_20221201_NO30_400nL_HLAc1_5e6_directIP_titration_rep1_Slot1-37_1_3502.8840.1540.2 2 2 1 13.04 8.1 6.21 4.94 89.1 7 1 1 11000.0 Hydrogen 42 (A) 9 SADPGNLKY (S) 9 SEGPGNIPPP S/A/D|P|G N/L|K\Y 77.8 7 4 6 3 0 dual b/y bias 30.540945 0.788213 482.7371 964.473 -0.0067 -7.0 0.0 5.55 48244.4 nuORFdb_human ENST00000522480.5_1_16:21481075-21513207:- ENST00000522480.5_1_16:21481075-21513207:-|ENST00000522841.6_1_16:21481075-21531198:- SMG1P3 Pseudogene Pseudogene GN=SMG1P3 Pseudogene E_20221201_NO30_400nL_HLAc1_5e6_directIP_titration_rep1_Slot1-37_1_3502 5mio-rep2 E_20221201_NO30_400nL_HLAc1_5e6_directIP_titration_rep2_Slot2-2_1_3510.8806.1579.2 2 2 1 13.1 7.92 6.51 5.18 87.5 8 1 1 7800.0 Hydrogen 42 (A) 9 SADPGNLKY (S) 9 SEGPGNIPPP S/A/D|P/G/N|L|K\Y 100.0 9 4 7 3 0 dual b/y bias 30.415688 0.763899 482.736 964.473 -0.0087 -9.0 -0.3000000000000007 5.55 48244.4 nuORFdb_human ENST00000522480.5_1_16:21481075-21513207:- ENST00000522480.5_1_16:21481075-21513207:-|ENST00000522841.6_1_16:21481075-21531198:- SMG1P3 Pseudogene Pseudogene GN=SMG1P3 Pseudogene E_20221201_NO30_400nL_HLAc1_5e6_directIP_titration_rep2_Slot2-2_1_3510 5mio-rep3 E_20221201_NO30_400nL_HLAc1_5e6_directIP_titration_rep3_Slot2-2_1_3511.8827.1578.2 2 4 1 12.92 7.16 7.02 5.76 90.2 7 1 1 16600.0 Hydrogen 42 (A) 9 SADPGNLKY (S) 9 SEGPGNIPPP S/A/D|P\G N|L\K\Y 77.8 7 5 4 2 0 unbiased 30.498901 0.764108 482.7367 964.473 -0.0067 -7.0 1.1999999999999993 5.55 48244.4 nuORFdb_human ENST00000522480.5_1_16:21481075-21513207:- ENST00000522480.5_1_16:21481075-21513207:-|ENST00000522841.6_1_16:21481075-21531198:- SMG1P3 Pseudogene Pseudogene GN=SMG1P3 Pseudogene E_20221201_NO30_400nL_HLAc1_5e6_directIP_titration_rep3_Slot2-2_1_3511 SCP E_20221201_NO30_400nL_HLAc1_4e7_PDAC_Slot2-6_1_3524.23939.424.1 1 5 1 12.72 8.38 5.8 4.34 93.9 6 0 0 61100.0 Hydrogen 14 (T) 9 SAIPDSLLY (G) 9 DPSLSALIY S A/I/P D|S\L\L\Y 55.6 5 4 3 1 0 unbiased 82.702759 1.471112 978.5125 978.514 -0.0012 -1.3 1.0999999999999999 3.8 7015.9 nuORFdb_human ENST00000548577.1_17:6915938-6917031:+ ENST00000548577.1_17:6915938-6917031:+ RNASEK Out-of-Frame Out-of-Frame GN=RNASEK Out-of-Frame E_20221201_NO30_400nL_HLAc1_4e7_PDAC_Slot2-6_1_3524 20mio-rep1 E_20221201_NO30_400nL_HLAc1_2e7_directIP_titration_rep1_Slot2-4_1_3515.13633.1607.2 2 1 1 8.81 5.64 3.17 3.17 62.6 5 1 1 6340.0 Hydrogen 33 (L) 8 SAPALKAL (T) 8 SAALKPAL S/A/P A|L K\A\L 50.0 4 3 3 1 0 unbiased 47.086769 0.746143 385.7387 770.477 -0.0063 -8.2 -1.9999999999999991 8.47 5809.6 nuORFdb_human ENST00000297350.8_1_8:119964236-119964404:- ENST00000297350.8_1_8:119964236-119964404:- TNFRSF11B 5' uORF 5' uORF GN=TNFRSF11B 5' uORF E_20221201_NO30_400nL_HLAc1_2e7_directIP_titration_rep1_Slot2-4_1_3515 40mio-rep1 E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep1_Slot1-04_1_3496.13889.1583.2 2 2 1 10.78 9.95 4.02 0.83 78.7 5 1 1 12400.0 Hydrogen 33 (L) 8 SAPALKAL (T) 8 ASPAKALL S/A/P A|L K\A\L 50.0 4 3 3 1 0 unbiased 47.976582 0.760904 385.7401 770.477 -0.0043 -5.6 -2.3999999999999995 8.47 5809.6 nuORFdb_human ENST00000297350.8_1_8:119964236-119964404:- ENST00000297350.8_1_8:119964236-119964404:- TNFRSF11B 5' uORF 5' uORF GN=TNFRSF11B 5' uORF E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep1_Slot1-04_1_3496 40mio-rep2 E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep2_Slot2-5_1_3520.13597.1602.2 2 3 1 11.69 9.6 3.06 2.09 83.0 5 1 1 48000.0 Hydrogen 33 (L) 8 SAPALKAL (T) 8 ASPAKALL S/A/P A|L K\A\L 50.0 4 3 3 1 0 unbiased 46.970558 0.749127 385.7401 770.477 -0.0043 -5.6 -0.6999999999999993 8.47 5809.6 nuORFdb_human ENST00000297350.8_1_8:119964236-119964404:- ENST00000297350.8_1_8:119964236-119964404:- TNFRSF11B 5' uORF 5' uORF GN=TNFRSF11B 5' uORF E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep2_Slot2-5_1_3520 40mio-rep3 E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep3_Slot2-5_1_3521.13601.1602.2 2 1 1 11.33 9.18 2.15 2.15 78.3 5 1 1 10900.0 Hydrogen 33 (L) 8 SAPALKAL (T) 8 SAALKPAL S/A/P A/L K\A\L 50.0 4 2 3 0 0 unbiased 46.987782 0.749054 385.7398 770.477 -0.0043 -5.6 -1.0 8.47 5809.6 nuORFdb_human ENST00000297350.8_1_8:119964236-119964404:- ENST00000297350.8_1_8:119964236-119964404:- TNFRSF11B 5' uORF 5' uORF GN=TNFRSF11B 5' uORF E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep3_Slot2-5_1_3521 40mio-rep4 E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep4_Slot2-5_1_3522.13603.1601.2 2 2 1 9.46 7.05 3.09 2.41 75.0 5 1 1 14200.0 Hydrogen 33 (L) 8 SAPALKAL (T) 8 ASPAKALL S/A/P A|L K\A\L 50.0 4 3 3 1 0 unbiased 46.978523 0.74994 385.74 770.477 -0.0043 -5.6 -0.8999999999999995 8.47 5809.6 nuORFdb_human ENST00000297350.8_1_8:119964236-119964404:- ENST00000297350.8_1_8:119964236-119964404:- TNFRSF11B 5' uORF 5' uORF GN=TNFRSF11B 5' uORF E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep4_Slot2-5_1_3522 Eva-50mio-3fr AL20210827_EV_HLA_PDAC_PANFRO413_2D_50e6_W632_classI_fxn2.21501.21501.2 2 1 1 8.46 5.15 3.21 3.31 70.1 6 1 1 2460000.0 Hydrogen 4 (R) 10 SASAARLAPL (R) 10 ASRTQAILP S A|S/A/A R\L\A|P L 40.0 4 4 4 2 0 unbiased 0.885 75.6 40.63 100.0 0.0 478.7809 956.552 0.0021 2.2 1.9000000000000001 9.47 1924.3 nuORFdb_human ENST00000269593.4_1_17:38599756-38599807:+ ENST00000269593.4_1_17:38599756-38599807:+ IGFBP4 5' uORF 5' uORF GN=IGFBP4 5' uORF AL20210827_EV_HLA_PDAC_PANFRO413_2D_50e6_W632_classI_fxn2 Exploris_FAIMS VH20221207_MKP_FAIMS2022E_HLAc1_4e7_PDAC.32623.32623.2 2 1 1 11.16 5.18 4.06 5.98 78.7 8 1 1 6940000.0 Hydrogen 4 (R) 10 SASAARLAPL (R) 10 GTSPRTKLP S A|S/A/A/R\L\A|P/L 80.0 8 4 6 2 0 unbiased 0.999 72.2 62.6 17.77 -50.0 478.7781 956.552 -0.0034 -3.6 -1.0 9.47 1924.3 nuORFdb_human ENST00000269593.4_1_17:38599756-38599807:+ ENST00000269593.4_1_17:38599756-38599807:+ IGFBP4 5' uORF 5' uORF GN=IGFBP4 5' uORF VH20221207_MKP_FAIMS2022E_HLAc1_4e7_PDAC Exploris_FAIMS VH20221207_MKP_FAIMS2022E_HLAc1_4e7_PDAC.26407.26407.2 2 1 1 12.54 8.92 2.52 3.62 78.7 6 1 1 3240000.0 Hydrogen 22 (T) 9 SASRVQAVL (L) 9 ASRSQGLVL S A\S-R\V\Q\A|V|L 77.8 7 6 2 2 1 b-ion bias 0.959 100.0 53.33 120.0 -50.0 465.7709 930.537 -0.0023 -2.4 0.20000000000000018 9.47 4071.5 nuORFdb_human ENST00000504673.5_1_3:141157084-141157195:+ ENST00000504673.5_1_3:141157084-141157195:+|ENST00000320339.8_1_X:118673380-118673455:- ZBTB38 5' uORF 5' uORF GN=ZBTB38 5' uORF VH20221207_MKP_FAIMS2022E_HLAc1_4e7_PDAC 1mio-rep1 E_20221201_NO30_400nL_HLAc1_1e6_directIP_titration_rep1_Slot1-06_1_3498.14015.1617.2 2 2 1 10.24 6.93 3.31 3.31 88.1 6 1 1 5480.0 Hydrogen 3 (K) 8 SASVKLAL (L) 8 SASKLGIL S/A/S/V/K L\A\L 75.0 6 2 4 0 0 y-ion bias 48.407814 0.739632 394.747 788.488 -0.0009 -1.2 3.7 8.47 1981.4 nuORFdb_human ENST00000367088.1_1_6:159057555-159057609:- ENST00000367088.1_1_6:159057555-159057609:- DYNLT1 3' dORF 3' dORF GN=DYNLT1 3' dORF E_20221201_NO30_400nL_HLAc1_1e6_directIP_titration_rep1_Slot1-06_1_3498 1mio-rep2 E_20221201_NO30_400nL_HLAc1_1e6_directIP_titration_rep3_Slot2-1_1_3508.14026.1726.2 2 1 1 8.3 5.55 2.25 2.74 73.3 7 1 1 1750.0 Hydrogen 3 (K) 8 SASVKLAL (L) 8 SASKLGIL S/A/S|V/K-L\A\L 100.0 8 3 4 1 1 unbiased 48.443493 0.669938 394.744 788.488 -0.0069 -8.8 -0.5 8.47 1981.4 nuORFdb_human ENST00000367088.1_1_6:159057555-159057609:- ENST00000367088.1_1_6:159057555-159057609:- DYNLT1 3' dORF 3' dORF GN=DYNLT1 3' dORF E_20221201_NO30_400nL_HLAc1_1e6_directIP_titration_rep3_Slot2-1_1_3508 10mio-rep1 E_20221201_NO30_400nL_HLAc1_1e7_directIP_titration_rep1_Slot2-3_1_3512.13705.1633.2 2 7 1 13.87 8.63 5.24 5.24 93.4 7 1 1 50900.0 Hydrogen 3 (K) 8 SASVKLAL (L) 8 SASKLGIL S/A/S/V|K-L\A\L 100.0 8 3 4 1 1 unbiased 47.346619 0.729079 394.7458 788.488 -0.0029 -3.7 4.0 8.47 1981.4 nuORFdb_human ENST00000367088.1_1_6:159057555-159057609:- ENST00000367088.1_1_6:159057555-159057609:- DYNLT1 3' dORF 3' dORF GN=DYNLT1 3' dORF E_20221201_NO30_400nL_HLAc1_1e7_directIP_titration_rep1_Slot2-3_1_3512 10mio-rep2 E_20221201_NO30_400nL_HLAc1_1e7_directIP_titration_rep2_Slot2-3_1_3513.13662.1651.2 2 4 1 13.3 6.99 6.32 6.32 90.4 7 1 1 18000.0 Hydrogen 3 (K) 8 SASVKLAL (L) 8 SASKLGIL S/A/S/V|K|L\A\L 100.0 8 4 5 2 0 unbiased 47.197567 0.71816 394.745 788.488 -0.0049 -6.2 0.5 8.47 1981.4 nuORFdb_human ENST00000367088.1_1_6:159057555-159057609:- ENST00000367088.1_1_6:159057555-159057609:- DYNLT1 3' dORF 3' dORF GN=DYNLT1 3' dORF E_20221201_NO30_400nL_HLAc1_1e7_directIP_titration_rep2_Slot2-3_1_3513 20mio-rep1 E_20221201_NO30_400nL_HLAc1_2e7_directIP_titration_rep1_Slot2-4_1_3515.13642.1625.2 2 6 1 14.86 9.6 5.26 5.26 92.7 7 1 1 90400.0 Hydrogen 3 (K) 8 SASVKLAL (L) 8 SASKLGIL S/A/S|V|K\L\A\L 100.0 8 5 4 2 0 unbiased 47.117821 0.734693 394.7453 788.488 -0.0049 -6.2 0.0 8.47 1981.4 nuORFdb_human ENST00000367088.1_1_6:159057555-159057609:- ENST00000367088.1_1_6:159057555-159057609:- DYNLT1 3' dORF 3' dORF GN=DYNLT1 3' dORF E_20221201_NO30_400nL_HLAc1_2e7_directIP_titration_rep1_Slot2-4_1_3515 20mio-rep2 E_20221201_NO30_400nL_HLAc1_2e7_directIP_titration_rep3_Slot2-4_1_3518.13719.1621.2 2 7 1 15.31 9.42 5.88 5.88 91.7 7 1 1 57800.0 Hydrogen 3 (K) 8 SASVKLAL (L) 8 SASKLGIL S/A/S|V/K\L\A\L 100.0 8 4 4 1 0 unbiased 47.385647 0.736753 394.7442 788.488 -0.0069 -8.8 -2.500000000000001 8.47 1981.4 nuORFdb_human ENST00000367088.1_1_6:159057555-159057609:- ENST00000367088.1_1_6:159057555-159057609:- DYNLT1 3' dORF 3' dORF GN=DYNLT1 3' dORF E_20221201_NO30_400nL_HLAc1_2e7_directIP_titration_rep3_Slot2-4_1_3518 20mio-rep3 E_20221201_NO30_400nL_HLAc1_2e7_directIP_titration_rep4_Slot2-4_1_3519.13635.1657.2 2 7 1 13.45 9.22 4.23 4.23 92.1 7 1 1 87800.0 Hydrogen 3 (K) 8 SASVKLAL (L) 8 ASSGKLLL S/A/S|V/K\L\A\L 100.0 8 4 4 1 0 unbiased 47.098164 0.71397 394.7442 788.488 -0.0069 -8.8 -3.000000000000001 8.47 1981.4 nuORFdb_human ENST00000367088.1_1_6:159057555-159057609:- ENST00000367088.1_1_6:159057555-159057609:- DYNLT1 3' dORF 3' dORF GN=DYNLT1 3' dORF E_20221201_NO30_400nL_HLAc1_2e7_directIP_titration_rep4_Slot2-4_1_3519 40mio-rep1 E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep1_Slot1-04_1_3496.13907.1606.2 2 5 1 14.34 8.99 5.35 5.35 92.7 7 1 1 53700.0 Hydrogen 3 (K) 8 SASVKLAL (L) 8 SASKLGIL S/A/S/V/K\L\A\L 100.0 8 3 4 0 0 unbiased 48.0396 0.746343 394.7458 788.488 -0.0029 -3.7 -0.5 8.47 1981.4 nuORFdb_human ENST00000367088.1_1_6:159057555-159057609:- ENST00000367088.1_1_6:159057555-159057609:- DYNLT1 3' dORF 3' dORF GN=DYNLT1 3' dORF E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep1_Slot1-04_1_3496 40mio-rep2 E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep2_Slot2-5_1_3520.13594.640.1 1 4 1 14.42 10.19 4.36 4.23 94.7 5 1 1 57400.0 Hydrogen 3 (K) 8 SASVKLAL (L) 8 VTSGKLAL S A S|V|K\L\A\L 62.5 5 5 2 2 0 b-ion bias 46.960194 1.342174 788.4844 788.488 -0.0036 -4.6 0.3000000000000007 8.47 1981.4 nuORFdb_human ENST00000367088.1_1_6:159057555-159057609:- ENST00000367088.1_1_6:159057555-159057609:- DYNLT1 3' dORF 3' dORF GN=DYNLT1 3' dORF E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep2_Slot2-5_1_3520 40mio-rep3 E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep3_Slot2-5_1_3521.13599.1622.2 2 3 1 14.56 9.05 5.51 5.51 93.6 6 1 1 26400.0 Hydrogen 3 (K) 8 SASVKLAL (L) 8 SASKLGIL S/A/S|V|K L\A\L 75.0 6 4 4 2 0 unbiased 46.980862 0.736669 394.7453 788.488 -0.0049 -6.2 -1.6000000000000005 8.47 1981.4 nuORFdb_human ENST00000367088.1_1_6:159057555-159057609:- ENST00000367088.1_1_6:159057555-159057609:- DYNLT1 3' dORF 3' dORF GN=DYNLT1 3' dORF E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep3_Slot2-5_1_3521 40mio-rep4 E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep4_Slot2-5_1_3522.13599.1618.2 2 4 1 12.93 8.53 4.39 4.39 92.1 6 1 1 29000.0 Hydrogen 3 (K) 8 SASVKLAL (L) 8 SASKLGIL S/A/S|V|K L\A\L 75.0 6 4 4 2 0 unbiased 46.964695 0.738877 394.7453 788.488 -0.0049 -6.2 -1.5 8.47 1981.4 nuORFdb_human ENST00000367088.1_1_6:159057555-159057609:- ENST00000367088.1_1_6:159057555-159057609:- DYNLT1 3' dORF 3' dORF GN=DYNLT1 3' dORF E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep4_Slot2-5_1_3522 5mio-rep1 E_20221201_NO30_400nL_HLAc1_5e6_directIP_titration_rep1_Slot1-37_1_3502.13976.1618.2 2 4 1 10.82 7.99 2.83 2.83 85.9 6 1 1 15000.0 Hydrogen 3 (K) 8 SASVKLAL (L) 8 SASKLGIL S/A/S/V|K L\A\L 75.0 6 3 4 1 0 unbiased 48.275753 0.738888 394.7448 788.488 -0.0049 -6.2 0.7999999999999998 8.47 1981.4 nuORFdb_human ENST00000367088.1_1_6:159057555-159057609:- ENST00000367088.1_1_6:159057555-159057609:- DYNLT1 3' dORF 3' dORF GN=DYNLT1 3' dORF E_20221201_NO30_400nL_HLAc1_5e6_directIP_titration_rep1_Slot1-37_1_3502 5mio-rep3 E_20221201_NO30_400nL_HLAc1_5e6_directIP_titration_rep3_Slot2-2_1_3511.13882.655.1 1 6 1 13.3 8.41 5.08 4.89 91.2 6 1 1 17500.0 Hydrogen 3 (K) 8 SASVKLAL (L) 8 VTSGKLAL S A/S|V|K\L\A\L 75.0 6 5 3 2 0 unbiased 47.953331 1.333234 788.4828 788.488 -0.0046 -5.9 2.299999999999999 8.47 1981.4 nuORFdb_human ENST00000367088.1_1_6:159057555-159057609:- ENST00000367088.1_1_6:159057555-159057609:- DYNLT1 3' dORF 3' dORF GN=DYNLT1 3' dORF E_20221201_NO30_400nL_HLAc1_5e6_directIP_titration_rep3_Slot2-2_1_3511 40mio-rep1 E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep1_Slot1-04_1_3496.14730.1532.2 2 1 1 8.68 5.13 3.34 3.55 68.1 7 1 1 6040.0 Hydrogen 21 (L) 9 SASVRGLAF (C) 9 ASSSKLPAF S/A/S/V/R G\L\A\F 77.8 7 3 4 0 0 unbiased 50.881649 0.793117 454.2504 907.5 -0.0069 -7.6 -4.3999999999999995 9.47 5693.3 nuORFdb_human ENST00000457535.5_1_1:110950486-110954860:+ ENST00000457535.5_1_1:110950486-110954860:+ LAMTOR5-AS1 Antisense lncRNA GN=LAMTOR5-AS1 Antisense E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep1_Slot1-04_1_3496 40mio-rep1 E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep1_Slot1-04_1_3496.11797.652.1 1 1 1 8.53 4.29 3.88 4.24 73.5 7 0 0 4620.0 Hydrogen 53 (S) 10 SAYGGALAGA (S) 10 FSGTNIAAG S A/Y/G G\A\L\A\G-A 60.0 6 4 2 0 1 b-ion bias 40.751381 1.335378 837.4045 837.41 -0.0051 -6.1 -2.8999999999999995 5.24 8452.1 nuORFdb_human ENST00000472734.1_1_1:211433019-211434404:+ ENST00000472734.1_1_1:211433019-211434404:+ RCOR3 5' Overlap uORF 5' Overlap uORF GN=RCOR3 5' Overlap uORF E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep1_Slot1-04_1_3496 40mio-rep3 E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep3_Slot2-5_1_3521.11495.666.1 1 1 1 7.41 5.04 2.24 2.37 73.6 6 0 0 4210.0 Hydrogen 53 (S) 10 SAYGGALAGA (S) 10 FSGTNIAAG S A/Y/G G A\L\A\G-A 50.0 5 3 2 0 1 unbiased 39.712101 1.326727 837.4062 837.41 -0.0041 -4.9 -0.3000000000000007 5.24 8452.1 nuORFdb_human ENST00000472734.1_1_1:211433019-211434404:+ ENST00000472734.1_1_1:211433019-211434404:+ RCOR3 5' Overlap uORF 5' Overlap uORF GN=RCOR3 5' Overlap uORF E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep3_Slot2-5_1_3521 40mio-rep4 E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep4_Slot2-5_1_3522.11500.666.1 1 1 1 8.97 3.22 3.29 5.75 74.6 7 0 0 5680.0 Hydrogen 53 (S) 10 SAYGGALAGA (S) 10 ASGFQSLQ S A/Y/G/G A\L\A\G\A 60.0 6 4 3 0 0 unbiased 39.714481 1.326842 837.4073 837.41 -0.0031 -3.7 1.0 5.24 8452.1 nuORFdb_human ENST00000472734.1_1_1:211433019-211434404:+ ENST00000472734.1_1_1:211433019-211434404:+ RCOR3 5' Overlap uORF 5' Overlap uORF GN=RCOR3 5' Overlap uORF E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep4_Slot2-5_1_3522 40mio-rep1 E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep1_Slot1-04_1_3496.15175.502.1 1 1 1 12.2 5.2 6.78 7.0 76.6 6 0 0 3880.0 Hydrogen 47 (F) 9 SEAEAGVQW (C) 9 NDLSDVQW S E/A E|A\G|V|Q\W 55.6 5 5 4 3 0 dual b/y bias 52.418571 1.42483 976.4316 976.437 -0.0051 -5.2 -2.0 3.79 7667.6 nuORFdb_human ENST00000420474.5_1_20:54963780-54967263:- ENST00000420474.5_1_20:54963780-54967263:-|ENST00000456249.5_1_20:54963780-54967263:- AURKA 5' uORF 5' uORF GN=AURKA 5' uORF E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep1_Slot1-04_1_3496 40mio-rep4 E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep4_Slot2-5_1_3522.16192.1414.2 2 1 1 7.37 4.78 1.05 2.59 54.1 5 1 1 11300.0 Hydrogen 14 (S) 10 SEALPSRVSF (F) 10 ATDIGNLFNK S E/A|L|P S R V\S\F 40.0 4 4 3 2 0 unbiased 55.918911 0.867405 546.7851 1092.568 -0.0057 -5.2 -0.5 5.72 3363.8 nuORFdb_human ENST00000455773.7_1_10:12238257-12240732:+ ENST00000455773.7_1_10:12238257-12240732:+ CDC123 ncRNA Processed Transcript lncRNA GN=CDC123 ncRNA Processed Transcript E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep4_Slot2-5_1_3522 20mio-rep1 E_20221201_NO30_400nL_HLAc1_2e7_directIP_titration_rep1_Slot2-4_1_3515.21605.1463.2 2 3 1 10.61 5.19 4.28 5.42 79.5 7 1 1 12300.0 Hydrogen 88 (G) 9 SEAPALRLW (A) 9 DTAKIFKGY S E|A|P/A/L/R\L\W 77.8 7 4 5 2 0 unbiased 74.609741 0.83703 521.7835 1042.568 -0.0093 -8.9 -2.7 5.72 10867.4 nuORFdb_human ENST00000535572.5_1_12:861818-862124:+ ENST00000535572.5_1_12:861818-862124:+ WNK1 5' uORF 5' uORF GN=WNK1 5' uORF E_20221201_NO30_400nL_HLAc1_2e7_directIP_titration_rep1_Slot2-4_1_3515 20mio-rep2 E_20221201_NO30_400nL_HLAc1_2e7_directIP_titration_rep3_Slot2-4_1_3518.21630.1462.2 2 2 1 12.2 5.12 7.0 7.07 78.9 8 1 1 9060.0 Hydrogen 88 (G) 9 SEAPALRLW (A) 9 SEAGKPAGARV S/E/A|P/A\L|R|L\W 100.0 9 5 6 3 0 dual b/y bias 74.713135 0.837665 521.7836 1042.568 -0.0073 -7.0 -0.7000000000000002 5.72 10867.4 nuORFdb_human ENST00000535572.5_1_12:861818-862124:+ ENST00000535572.5_1_12:861818-862124:+ WNK1 5' uORF 5' uORF GN=WNK1 5' uORF E_20221201_NO30_400nL_HLAc1_2e7_directIP_titration_rep3_Slot2-4_1_3518 20mio-rep3 E_20221201_NO30_400nL_HLAc1_2e7_directIP_titration_rep4_Slot2-4_1_3519.21654.1465.2 2 1 1 11.49 6.26 5.22 5.22 85.1 7 1 1 4550.0 Hydrogen 88 (G) 9 SEAPALRLW (A) 9 SEAPIYIHL S E/A|P|A/L|R/L\W 77.8 7 4 6 3 0 dual b/y bias 74.787651 0.835572 521.7852 1042.568 -0.0053 -5.1 0.7000000000000002 5.72 10867.4 nuORFdb_human ENST00000535572.5_1_12:861818-862124:+ ENST00000535572.5_1_12:861818-862124:+ WNK1 5' uORF 5' uORF GN=WNK1 5' uORF E_20221201_NO30_400nL_HLAc1_2e7_directIP_titration_rep4_Slot2-4_1_3519 40mio-rep1 E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep1_Slot1-04_1_3496.21847.1430.2 2 3 1 11.29 6.96 4.32 4.32 84.2 6 1 1 20900.0 Hydrogen 88 (G) 9 SEAPALRLW (A) 9 SEAPIYIHL S E|A|P|A/L/R|L W 55.6 5 4 6 4 0 dual b/y bias 75.470337 0.857634 521.7856 1042.568 -0.0033 -3.1 0.10000000000000009 5.72 10867.4 nuORFdb_human ENST00000535572.5_1_12:861818-862124:+ ENST00000535572.5_1_12:861818-862124:+ WNK1 5' uORF 5' uORF GN=WNK1 5' uORF E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep1_Slot1-04_1_3496 40mio-rep2 E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep2_Slot2-5_1_3520.21625.1459.2 2 1 1 10.14 5.87 4.27 4.27 79.3 6 1 1 6250.0 Hydrogen 88 (G) 9 SEAPALRLW (A) 9 SEAPIYIHL S E|A|P/A/L|R L\W 55.6 5 4 5 3 0 dual b/y bias 74.705231 0.839539 521.7842 1042.568 -0.0073 -7.0 -2.0999999999999996 5.72 10867.4 nuORFdb_human ENST00000535572.5_1_12:861818-862124:+ ENST00000535572.5_1_12:861818-862124:+ WNK1 5' uORF 5' uORF GN=WNK1 5' uORF E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep2_Slot2-5_1_3520 40mio-rep3 E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep3_Slot2-5_1_3521.21653.1459.2 2 2 1 11.35 6.13 5.22 5.22 82.2 7 1 1 12300.0 Hydrogen 88 (G) 9 SEAPALRLW (A) 9 SEAPIYIHL S E|A|P/A/L/R/L\W 77.8 7 3 6 2 0 y-ion bias 74.800102 0.839346 521.7843 1042.568 -0.0073 -7.0 -2.4000000000000004 5.72 10867.4 nuORFdb_human ENST00000535572.5_1_12:861818-862124:+ ENST00000535572.5_1_12:861818-862124:+ WNK1 5' uORF 5' uORF GN=WNK1 5' uORF E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep3_Slot2-5_1_3521 40mio-rep4 E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep4_Slot2-5_1_3522.21655.1450.2 2 2 1 9.89 4.49 5.4 5.4 76.8 7 1 1 11100.0 Hydrogen 88 (G) 9 SEAPALRLW (A) 9 SEAPIYIHL S E/A|P/A/L|R/L-W 77.8 7 2 6 2 1 y-ion bias 74.788841 0.844957 521.7836 1042.568 -0.0073 -7.0 -2.3 5.72 10867.4 nuORFdb_human ENST00000535572.5_1_12:861818-862124:+ ENST00000535572.5_1_12:861818-862124:+ WNK1 5' uORF 5' uORF GN=WNK1 5' uORF E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep4_Slot2-5_1_3522 40mio-rep2 E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep2_Slot2-5_1_3520.10704.620.1 1 1 1 7.61 5.41 2.2 2.2 78.7 6 0 0 3290.0 Hydrogen 119 (G) 9 SEAPGTTSF (P) 9 ESANIESF S E/A/P G\T\T\S-F 55.6 5 3 2 0 1 unbiased 36.978703 1.354544 896.3932 896.4 -0.0066 -7.4 -2.5 4.0 13658.0 nuORFdb_human ENST00000357814.7_2_17:72767432-72767837:- ENST00000357814.7_2_17:72767432-72767837:- NAT9 3' dORF 3' dORF GN=NAT9 3' dORF E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep2_Slot2-5_1_3520 1mio-rep1 E_20221201_NO30_400nL_HLAc1_1e6_directIP_titration_rep1_Slot1-06_1_3498.11831.1518.2 2 2 1 11.8 8.18 3.65 3.62 80.7 6 1 1 7040.0 Hydrogen 26 (G) 9 SEAPSKITL (C) 9 nTALARWL S/E|A|P|S-K\I T\L 77.8 7 5 4 3 1 dual b/y bias 40.868675 0.802369 473.2645 945.525 -0.0024 -2.6 2.3000000000000003 5.72 5548.8 nuORFdb_human ENST00000234170.9_1_2:37456133-37458643:- ENST00000234170.9_1_2:37456133-37458643:- CEBPZ Out-of-Frame Out-of-Frame GN=CEBPZ Out-of-Frame E_20221201_NO30_400nL_HLAc1_1e6_directIP_titration_rep1_Slot1-06_1_3498 10mio-rep1 E_20221201_NO30_400nL_HLAc1_1e7_directIP_titration_rep1_Slot2-3_1_3512.11496.1549.2 2 1 1 8.07 5.8 2.41 2.26 61.3 6 1 1 5100.0 Hydrogen 26 (G) 9 SEAPSKITL (C) 9 SEAALLQTL S E|A/P/S K-I\T\L 55.6 5 3 3 1 1 unbiased 39.717995 0.782662 473.2622 945.525 -0.0084 -8.9 -1.2000000000000002 5.72 5548.8 nuORFdb_human ENST00000234170.9_1_2:37456133-37458643:- ENST00000234170.9_1_2:37456133-37458643:- CEBPZ Out-of-Frame Out-of-Frame GN=CEBPZ Out-of-Frame E_20221201_NO30_400nL_HLAc1_1e7_directIP_titration_rep1_Slot2-3_1_3512 10mio-rep2 E_20221201_NO30_400nL_HLAc1_1e7_directIP_titration_rep2_Slot2-3_1_3513.11443.1527.2 2 2 1 14.48 11.29 4.91 3.19 85.1 7 1 1 14700.0 Hydrogen 26 (G) 9 SEAPSKITL (C) 9 SEAALLQTL S/E/A|P/S K\I|T\L 77.8 7 4 5 2 0 unbiased 39.533855 0.796561 473.2622 945.525 -0.0084 -8.9 -2.2 5.72 5548.8 nuORFdb_human ENST00000234170.9_1_2:37456133-37458643:- ENST00000234170.9_1_2:37456133-37458643:- CEBPZ Out-of-Frame Out-of-Frame GN=CEBPZ Out-of-Frame E_20221201_NO30_400nL_HLAc1_1e7_directIP_titration_rep2_Slot2-3_1_3513 20mio-rep2 E_20221201_NO30_400nL_HLAc1_2e7_directIP_titration_rep3_Slot2-4_1_3518.11541.1519.2 2 3 1 14.76 12.16 4.87 2.6 93.1 6 1 1 71800.0 Hydrogen 26 (G) 9 SEAPSKITL (C) 9 SEAALLQTL S E|A|P|S-K-I\T\L 77.8 7 5 3 3 2 dual b/y bias 39.86338 0.801316 473.2631 945.525 -0.0064 -6.8 -0.5 5.72 5548.8 nuORFdb_human ENST00000234170.9_1_2:37456133-37458643:- ENST00000234170.9_1_2:37456133-37458643:- CEBPZ Out-of-Frame Out-of-Frame GN=CEBPZ Out-of-Frame E_20221201_NO30_400nL_HLAc1_2e7_directIP_titration_rep3_Slot2-4_1_3518 20mio-rep3 E_20221201_NO30_400nL_HLAc1_2e7_directIP_titration_rep4_Slot2-4_1_3519.11431.1519.2 2 1 1 14.07 11.42 4.83 2.66 88.5 6 1 1 46400.0 Hydrogen 26 (G) 9 SEAPSKITL (C) 9 SEAALLQTL S E|A|P/S K-I|T\L 55.6 5 4 4 3 1 dual b/y bias 39.489326 0.801709 473.2636 945.525 -0.0044 -4.7 1.0999999999999996 5.72 5548.8 nuORFdb_human ENST00000234170.9_1_2:37456133-37458643:- ENST00000234170.9_1_2:37456133-37458643:- CEBPZ Out-of-Frame Out-of-Frame GN=CEBPZ Out-of-Frame E_20221201_NO30_400nL_HLAc1_2e7_directIP_titration_rep4_Slot2-4_1_3519 40mio-rep1 E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep1_Slot1-04_1_3496.11685.1502.2 2 3 1 14.56 12.95 5.42 1.6 87.7 6 1 1 50600.0 Hydrogen 26 (G) 9 SEAPSKITL (C) 9 SEAALLQTL S E|A|P/S K-I|T\L 55.6 5 4 4 3 1 dual b/y bias 40.364712 0.812159 473.265 945.525 -0.0024 -2.6 0.6000000000000001 5.72 5548.8 nuORFdb_human ENST00000234170.9_1_2:37456133-37458643:- ENST00000234170.9_1_2:37456133-37458643:- CEBPZ Out-of-Frame Out-of-Frame GN=CEBPZ Out-of-Frame E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep1_Slot1-04_1_3496 40mio-rep2 E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep2_Slot2-5_1_3520.11398.1514.2 2 2 1 16.71 13.54 5.48 3.16 91.7 6 1 1 92700.0 Hydrogen 26 (G) 9 SEAPSKITL (C) 9 SEAALLQTL S E|A|P/S K\I|T\L 55.6 5 5 4 3 0 dual b/y bias 39.374104 0.804602 473.2639 945.525 -0.0044 -4.7 0.20000000000000018 5.72 5548.8 nuORFdb_human ENST00000234170.9_1_2:37456133-37458643:- ENST00000234170.9_1_2:37456133-37458643:- CEBPZ Out-of-Frame Out-of-Frame GN=CEBPZ Out-of-Frame E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep2_Slot2-5_1_3520 40mio-rep3 E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep3_Slot2-5_1_3521.11398.1515.2 2 2 1 16.78 14.12 4.97 2.67 91.7 7 1 1 95700.0 Hydrogen 26 (G) 9 SEAPSKITL (C) 9 SEAALLQTL S/E|A|P/S K\I\T\L 77.8 7 5 4 2 0 unbiased 39.377323 0.803866 473.2636 945.525 -0.0044 -4.7 -0.10000000000000053 5.72 5548.8 nuORFdb_human ENST00000234170.9_1_2:37456133-37458643:- ENST00000234170.9_1_2:37456133-37458643:- CEBPZ Out-of-Frame Out-of-Frame GN=CEBPZ Out-of-Frame E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep3_Slot2-5_1_3521 40mio-rep4 E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep4_Slot2-5_1_3522.11398.1513.2 2 1 1 16.06 14.48 5.86 1.58 87.7 7 1 1 28700.0 Hydrogen 26 (G) 9 SEAPSKITL (C) 9 SEAALLQTL S/E|A|P/S K-I|T\L 77.8 7 4 5 3 1 dual b/y bias 39.362411 0.805451 473.2637 945.525 -0.0044 -4.7 0.0 5.72 5548.8 nuORFdb_human ENST00000234170.9_1_2:37456133-37458643:- ENST00000234170.9_1_2:37456133-37458643:- CEBPZ Out-of-Frame Out-of-Frame GN=CEBPZ Out-of-Frame E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep4_Slot2-5_1_3522 5mio-rep1 E_20221201_NO30_400nL_HLAc1_5e6_directIP_titration_rep1_Slot1-37_1_3502.11746.1517.2 2 2 1 13.55 9.82 4.94 3.73 87.5 7 1 1 9590.0 Hydrogen 26 (G) 9 SEAPSKITL (C) 9 TnAGILAATI S/E/A|P|S-K-I\T\L 100.0 9 4 4 2 2 unbiased 40.575962 0.802624 473.2609 945.525 -0.0104 -11.0 -4.0 5.72 5548.8 nuORFdb_human ENST00000234170.9_1_2:37456133-37458643:- ENST00000234170.9_1_2:37456133-37458643:- CEBPZ Out-of-Frame Out-of-Frame GN=CEBPZ Out-of-Frame E_20221201_NO30_400nL_HLAc1_5e6_directIP_titration_rep1_Slot1-37_1_3502 5mio-rep2 E_20221201_NO30_400nL_HLAc1_5e6_directIP_titration_rep2_Slot2-2_1_3510.11661.1523.2 2 2 1 12.14 9.49 3.73 2.66 88.4 6 1 1 5530.0 Hydrogen 26 (G) 9 SEAPSKITL (C) 9 SEAALLQTL S E/A|P/S K-I\T\L 55.6 5 3 3 1 1 unbiased 40.277699 0.79881 473.2621 945.525 -0.0084 -8.9 -0.20000000000000107 5.72 5548.8 nuORFdb_human ENST00000234170.9_1_2:37456133-37458643:- ENST00000234170.9_1_2:37456133-37458643:- CEBPZ Out-of-Frame Out-of-Frame GN=CEBPZ Out-of-Frame E_20221201_NO30_400nL_HLAc1_5e6_directIP_titration_rep2_Slot2-2_1_3510 20mio-rep1 E_20221201_NO30_400nL_HLAc1_2e7_directIP_titration_rep1_Slot2-4_1_3515.11177.1531.2 2 1 1 7.51 3.23 3.79 4.29 63.8 7 1 1 3270.0 Hydrogen 38 (S) 9 SEASLSAKF (G) 9 SEAPLFIY S/E/A|S|L-S/A K-F 77.8 7 2 5 2 2 y-ion bias 38.605095 0.794121 470.2402 939.478 -0.0055 -5.8 0.40000000000000036 5.72 11180.6 nuORFdb_human ENST00000552321.1_17:6917030-6917732:+ ENST00000552321.1_17:6917030-6917732:+ RNASEK 3' dORF 3' dORF GN=RNASEK 3' dORF E_20221201_NO30_400nL_HLAc1_2e7_directIP_titration_rep1_Slot2-4_1_3515 40mio-rep1 E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep1_Slot1-04_1_3496.11479.1503.2 2 1 1 8.51 3.62 4.92 4.89 66.2 6 1 1 8460.0 Hydrogen 38 (S) 9 SEASLSAKF (G) 9 TnAGKSTLF S E|A|S|L S/A/K\F 55.6 5 4 5 3 0 dual b/y bias 39.652916 0.81174 470.2407 939.478 -0.0035 -3.7 -0.5 5.72 11180.6 nuORFdb_human ENST00000552321.1_17:6917030-6917732:+ ENST00000552321.1_17:6917030-6917732:+ RNASEK 3' dORF 3' dORF GN=RNASEK 3' dORF E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep1_Slot1-04_1_3496 40mio-rep2 E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep2_Slot2-5_1_3520.11176.1518.2 2 1 1 10.63 5.76 5.64 4.86 70.0 6 1 1 7520.0 Hydrogen 38 (S) 9 SEASLSAKF (G) 9 TnAGKSTLF S E|A|S|L S/A\K\F 55.6 5 5 4 3 0 dual b/y bias 38.607807 0.801911 470.2388 939.478 -0.0075 -7.9 -3.0 5.72 11180.6 nuORFdb_human ENST00000552321.1_17:6917030-6917732:+ ENST00000552321.1_17:6917030-6917732:+ RNASEK 3' dORF 3' dORF GN=RNASEK 3' dORF E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep2_Slot2-5_1_3520 40mio-rep3 E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep3_Slot2-5_1_3521.11166.1517.2 2 2 1 11.34 5.09 7.29 6.25 75.3 8 1 1 11200.0 Hydrogen 38 (S) 9 SEASLSAKF (G) 9 SEKEGPHR S/E/A|S|L\S/A\K\F 100.0 9 5 5 2 0 unbiased 38.575893 0.802534 470.2388 939.478 -0.0075 -7.9 -3.3000000000000007 5.72 11180.6 nuORFdb_human ENST00000552321.1_17:6917030-6917732:+ ENST00000552321.1_17:6917030-6917732:+ RNASEK 3' dORF 3' dORF GN=RNASEK 3' dORF E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep3_Slot2-5_1_3521 40mio-rep4 E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep4_Slot2-5_1_3522.11185.1513.2 2 1 1 10.33 4.49 5.64 5.85 74.9 7 1 1 6370.0 Hydrogen 38 (S) 9 SEASLSAKF (G) 9 TnAGKSTLF S E|A|S|L\S/A\K\F 77.8 7 6 4 3 0 dual b/y bias 38.627277 0.805127 470.24 939.478 -0.0055 -5.8 -1.0999999999999996 5.72 11180.6 nuORFdb_human ENST00000552321.1_17:6917030-6917732:+ ENST00000552321.1_17:6917030-6917732:+ RNASEK 3' dORF 3' dORF GN=RNASEK 3' dORF E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep4_Slot2-5_1_3522 10mio-rep1 E_20221201_NO30_400nL_HLAc1_1e7_directIP_titration_rep1_Slot2-3_1_3512.19966.491.1 1 1 1 10.73 2.65 6.23 8.08 78.8 6 0 0 1140.0 Hydrogen 34 (G) 9 SEEPGSPLW (S) 9 ADLPTASPnn S E/E|P G/S|P|L\W 55.6 5 4 5 3 0 dual b/y bias 68.96994 1.431609 1001.4513 1001.457 -0.0075 -7.4 0.2999999999999998 3.79 4988.4 nuORFdb_human ENST00000547570.5_1_12:56708731-56709515:- ENST00000547570.5_1_12:56708731-56709515:-|ENST00000551475.5_3_12:56708731-56709747:-|ENST00000547423.5_3_12:56708731-56709751:-|ENST00000546388.1_1_12:56708912-56709661:- CNPY2 5' Overlap uORF 5' Overlap uORF GN=CNPY2 5' Overlap uORF E_20221201_NO30_400nL_HLAc1_1e7_directIP_titration_rep1_Slot2-3_1_3512 20mio-rep1 E_20221201_NO30_400nL_HLAc1_2e7_directIP_titration_rep1_Slot2-4_1_3515.19832.489.1 1 1 1 9.32 3.49 4.13 5.83 80.6 6 0 0 2410.0 Hydrogen 34 (G) 9 SEEPGSPLW (S) 9 EDTPLPGSW S E/E/P G/S|P\L\W 55.6 5 3 4 1 0 unbiased 68.488617 1.432749 1001.452 1001.457 -0.0075 -7.4 -1.2000000000000002 3.79 4988.4 nuORFdb_human ENST00000547570.5_1_12:56708731-56709515:- ENST00000547570.5_1_12:56708731-56709515:-|ENST00000551475.5_3_12:56708731-56709747:-|ENST00000547423.5_3_12:56708731-56709751:-|ENST00000546388.1_1_12:56708912-56709661:- CNPY2 5' Overlap uORF 5' Overlap uORF GN=CNPY2 5' Overlap uORF E_20221201_NO30_400nL_HLAc1_2e7_directIP_titration_rep1_Slot2-4_1_3515 20mio-rep2 E_20221201_NO30_400nL_HLAc1_2e7_directIP_titration_rep3_Slot2-4_1_3518.19911.484.1 1 2 1 10.08 2.9 5.08 7.18 84.2 6 0 0 4170.0 Hydrogen 34 (G) 9 SEEPGSPLW (S) 9 EDTPLPGSW S E/E/P G\S|P\L\W 55.6 5 4 3 1 0 unbiased 68.777428 1.43577 1001.454 1001.457 -0.0075 -7.4 -1.1000000000000005 3.79 4988.4 nuORFdb_human ENST00000547570.5_1_12:56708731-56709515:- ENST00000547570.5_1_12:56708731-56709515:-|ENST00000551475.5_3_12:56708731-56709747:-|ENST00000547423.5_3_12:56708731-56709751:-|ENST00000546388.1_1_12:56708912-56709661:- CNPY2 5' Overlap uORF 5' Overlap uORF GN=CNPY2 5' Overlap uORF E_20221201_NO30_400nL_HLAc1_2e7_directIP_titration_rep3_Slot2-4_1_3518 20mio-rep3 E_20221201_NO30_400nL_HLAc1_2e7_directIP_titration_rep4_Slot2-4_1_3519.19865.478.1 1 1 1 10.41 2.62 3.98 7.79 80.6 7 0 0 2530.0 Hydrogen 34 (G) 9 SEEPGSPLW (S) 9 EDTPLPGSW S E/E/P\G/S|P|L\W 77.8 7 4 5 2 0 unbiased 68.608955 1.439267 1001.454 1001.457 -0.0075 -7.4 -1.6000000000000005 3.79 4988.4 nuORFdb_human ENST00000547570.5_1_12:56708731-56709515:- ENST00000547570.5_1_12:56708731-56709515:-|ENST00000551475.5_3_12:56708731-56709747:-|ENST00000547423.5_3_12:56708731-56709751:-|ENST00000546388.1_1_12:56708912-56709661:- CNPY2 5' Overlap uORF 5' Overlap uORF GN=CNPY2 5' Overlap uORF E_20221201_NO30_400nL_HLAc1_2e7_directIP_titration_rep4_Slot2-4_1_3519 40mio-rep1 E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep1_Slot1-04_1_3496.20123.454.1 1 2 1 12.24 3.02 5.57 9.22 88.0 7 0 0 16800.0 Hydrogen 34 (G) 9 SEEPGSPLW (S) 9 EDTPLPGSW S E/E/P\G|S|P\L\W 77.8 7 5 4 2 0 unbiased 69.515289 1.453089 1001.4525 1001.457 -0.0075 -7.4 -4.2 3.79 4988.4 nuORFdb_human ENST00000547570.5_1_12:56708731-56709515:- ENST00000547570.5_1_12:56708731-56709515:-|ENST00000551475.5_3_12:56708731-56709747:-|ENST00000547423.5_3_12:56708731-56709751:-|ENST00000546388.1_1_12:56708912-56709661:- CNPY2 5' Overlap uORF 5' Overlap uORF GN=CNPY2 5' Overlap uORF E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep1_Slot1-04_1_3496 40mio-rep2 E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep2_Slot2-5_1_3520.19810.476.1 1 2 1 9.44 3.42 4.42 6.02 79.3 7 0 0 9590.0 Hydrogen 34 (G) 9 SEEPGSPLW (S) 9 EDTPLPGSW S E/E/P\G/S|P\L\W 77.8 7 4 4 1 0 unbiased 68.434166 1.4403 1001.4519 1001.457 -0.0075 -7.4 -2.5 3.79 4988.4 nuORFdb_human ENST00000547570.5_1_12:56708731-56709515:- ENST00000547570.5_1_12:56708731-56709515:-|ENST00000551475.5_3_12:56708731-56709747:-|ENST00000547423.5_3_12:56708731-56709751:-|ENST00000546388.1_1_12:56708912-56709661:- CNPY2 5' Overlap uORF 5' Overlap uORF GN=CNPY2 5' Overlap uORF E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep2_Slot2-5_1_3520 1mio-rep1 E_20221201_NO30_400nL_HLAc1_1e6_directIP_titration_rep1_Slot1-06_1_3498.12567.1420.2 2 1 1 9.8 6.4 4.0 3.4 76.0 6 1 1 4470.0 Hydrogen 56 (Y) 9 SEFDNRTSW (Q) 9 TDCLSSSVSW S E|F/D/N R\T|S\W 55.6 5 4 4 2 0 unbiased 43.409496 0.863627 571.2448 1141.491 -0.0082 -7.1 -2.1999999999999993 4.37 11377.6 nuORFdb_human ENST00000477091.5_1_21:35445925-35497723:+ ENST00000477091.5_1_21:35445925-35497723:+|ENST00000381151.4_2_21:35475142-35475211:+ MRPS6 3' Overlap dORF 3' Overlap dORF GN=MRPS6 3' Overlap dORF E_20221201_NO30_400nL_HLAc1_1e6_directIP_titration_rep1_Slot1-06_1_3498 10mio-rep1 E_20221201_NO30_400nL_HLAc1_1e7_directIP_titration_rep1_Slot2-3_1_3512.12204.1433.2 2 1 1 11.24 4.48 5.83 6.76 80.7 7 1 1 6990.0 Hydrogen 56 (Y) 9 SEFDNRTSW (Q) 9 GGcRQKGTSE S E/F|D/N\R\T|S\W 77.8 7 5 4 2 0 unbiased 42.162865 0.855525 571.2433 1141.491 -0.0122 -10.6 -2.8999999999999995 4.37 11377.6 nuORFdb_human ENST00000477091.5_1_21:35445925-35497723:+ ENST00000477091.5_1_21:35445925-35497723:+|ENST00000381151.4_2_21:35475142-35475211:+ MRPS6 3' Overlap dORF 3' Overlap dORF GN=MRPS6 3' Overlap dORF E_20221201_NO30_400nL_HLAc1_1e7_directIP_titration_rep1_Slot2-3_1_3512 10mio-rep2 E_20221201_NO30_400nL_HLAc1_1e7_directIP_titration_rep2_Slot2-3_1_3513.12200.1451.2 2 3 1 13.9 5.93 7.22 7.97 86.3 8 1 1 20800.0 Hydrogen 56 (Y) 9 SEFDNRTSW (Q) 9 SESSSGPAPHGE S/E/F|D|N\R\T|S\W 100.0 9 6 5 3 0 dual b/y bias 42.149342 0.84463 571.2449 1141.491 -0.0082 -7.1 -0.39999999999999947 4.37 11377.6 nuORFdb_human ENST00000477091.5_1_21:35445925-35497723:+ ENST00000477091.5_1_21:35445925-35497723:+|ENST00000381151.4_2_21:35475142-35475211:+ MRPS6 3' Overlap dORF 3' Overlap dORF GN=MRPS6 3' Overlap dORF E_20221201_NO30_400nL_HLAc1_1e7_directIP_titration_rep2_Slot2-3_1_3513 10mio-rep3 E_20221201_NO30_400nL_HLAc1_1e7_directIP_titration_rep3_Slot2-3_1_3514.12147.1436.2 2 2 1 11.56 7.13 3.9 4.44 78.4 8 1 1 17300.0 Hydrogen 56 (Y) 9 SEFDNRTSW (Q) 9 TDCLSSSVSW S/E/F/D/N\R\T|S\W 100.0 9 4 5 1 0 unbiased 41.966606 0.853875 571.2444 1141.491 -0.0102 -8.9 -2.6000000000000005 4.37 11377.6 nuORFdb_human ENST00000477091.5_1_21:35445925-35497723:+ ENST00000477091.5_1_21:35445925-35497723:+|ENST00000381151.4_2_21:35475142-35475211:+ MRPS6 3' Overlap dORF 3' Overlap dORF GN=MRPS6 3' Overlap dORF E_20221201_NO30_400nL_HLAc1_1e7_directIP_titration_rep3_Slot2-3_1_3514 20mio-rep1 E_20221201_NO30_400nL_HLAc1_2e7_directIP_titration_rep1_Slot2-4_1_3515.12107.1421.2 2 4 1 16.84 7.54 7.23 9.31 87.2 8 1 1 34700.0 Hydrogen 56 (Y) 9 SEFDNRTSW (Q) 9 SESSSGPAPHGE S/E|F|D|N\R|T|S\W 100.0 9 7 6 5 0 dual b/y bias 41.81834 0.86303 571.2474 1141.491 -0.0042 -3.6 2.6 4.37 11377.6 nuORFdb_human ENST00000477091.5_1_21:35445925-35497723:+ ENST00000477091.5_1_21:35445925-35497723:+|ENST00000381151.4_2_21:35475142-35475211:+ MRPS6 3' Overlap dORF 3' Overlap dORF GN=MRPS6 3' Overlap dORF E_20221201_NO30_400nL_HLAc1_2e7_directIP_titration_rep1_Slot2-4_1_3515 20mio-rep2 E_20221201_NO30_400nL_HLAc1_2e7_directIP_titration_rep3_Slot2-4_1_3518.12255.1430.2 2 3 1 13.64 5.94 7.19 7.7 86.4 7 1 1 23700.0 Hydrogen 56 (Y) 9 SEFDNRTSW (Q) 9 SESSSGPAPHGE S/E/F|D|N R|T|S\W 77.8 7 5 6 4 0 dual b/y bias 42.33065 0.857529 571.2462 1141.491 -0.0062 -5.4 0.8999999999999995 4.37 11377.6 nuORFdb_human ENST00000477091.5_1_21:35445925-35497723:+ ENST00000477091.5_1_21:35445925-35497723:+|ENST00000381151.4_2_21:35475142-35475211:+ MRPS6 3' Overlap dORF 3' Overlap dORF GN=MRPS6 3' Overlap dORF E_20221201_NO30_400nL_HLAc1_2e7_directIP_titration_rep3_Slot2-4_1_3518 20mio-rep3 E_20221201_NO30_400nL_HLAc1_2e7_directIP_titration_rep4_Slot2-4_1_3519.12131.1426.2 2 2 1 21.04 8.97 8.79 12.07 95.5 8 1 1 15600.0 Hydrogen 56 (Y) 9 SEFDNRTSW (Q) 9 TDCLSSSVSW S/E|F|D|N\R|T|S|W 100.0 9 7 7 6 0 dual b/y bias 41.90572 0.859743 571.2454 1141.491 -0.0082 -7.1 -1.2999999999999998 4.37 11377.6 nuORFdb_human ENST00000477091.5_1_21:35445925-35497723:+ ENST00000477091.5_1_21:35445925-35497723:+|ENST00000381151.4_2_21:35475142-35475211:+ MRPS6 3' Overlap dORF 3' Overlap dORF GN=MRPS6 3' Overlap dORF E_20221201_NO30_400nL_HLAc1_2e7_directIP_titration_rep4_Slot2-4_1_3519 40mio-rep1 E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep1_Slot1-04_1_3496.12465.1385.2 2 2 1 18.14 9.58 7.21 8.56 95.5 7 1 1 125000.0 Hydrogen 56 (Y) 9 SEFDNRTSW (Q) 9 TDCLSSSVSW S/E|F|D|N R|T|S\W 77.8 7 6 6 5 0 dual b/y bias 43.058121 0.885522 571.2471 1141.491 -0.0042 -3.6 -0.3999999999999999 4.37 11377.6 nuORFdb_human ENST00000477091.5_1_21:35445925-35497723:+ ENST00000477091.5_1_21:35445925-35497723:+|ENST00000381151.4_2_21:35475142-35475211:+ MRPS6 3' Overlap dORF 3' Overlap dORF GN=MRPS6 3' Overlap dORF E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep1_Slot1-04_1_3496 40mio-rep2 E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep2_Slot2-5_1_3520.12102.1414.2 2 1 1 19.79 10.23 8.75 9.56 95.7 8 1 1 77000.0 Hydrogen 56 (Y) 9 SEFDNRTSW (Q) 9 TDCLSSSVSW S/E|F|D|N\R|T|S\W 100.0 9 7 6 5 0 dual b/y bias 41.804996 0.867547 571.2463 1141.491 -0.0062 -5.4 -0.5 4.37 11377.6 nuORFdb_human ENST00000477091.5_1_21:35445925-35497723:+ ENST00000477091.5_1_21:35445925-35497723:+|ENST00000381151.4_2_21:35475142-35475211:+ MRPS6 3' Overlap dORF 3' Overlap dORF GN=MRPS6 3' Overlap dORF E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep2_Slot2-5_1_3520 40mio-rep3 E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep3_Slot2-5_1_3521.12104.1414.2 2 2 1 18.01 9.23 7.52 8.78 95.1 7 1 1 71200.0 Hydrogen 56 (Y) 9 SEFDNRTSW (Q) 9 TDCLSSSVSW S/E|F|D|N R|T|S\W 77.8 7 6 6 5 0 dual b/y bias 41.815228 0.867668 571.246 1141.491 -0.0062 -5.4 -0.8000000000000007 4.37 11377.6 nuORFdb_human ENST00000477091.5_1_21:35445925-35497723:+ ENST00000477091.5_1_21:35445925-35497723:+|ENST00000381151.4_2_21:35475142-35475211:+ MRPS6 3' Overlap dORF 3' Overlap dORF GN=MRPS6 3' Overlap dORF E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep3_Slot2-5_1_3521 40mio-rep4 E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep4_Slot2-5_1_3522.12103.1417.2 2 2 1 19.09 8.86 9.05 10.24 96.7 8 1 1 57900.0 Hydrogen 56 (Y) 9 SEFDNRTSW (Q) 9 TDCLSSSVSW S/E|F|D|N\R|T|S\W 100.0 9 7 6 5 0 dual b/y bias 41.796333 0.865622 571.2458 1141.491 -0.0062 -5.4 -0.7000000000000002 4.37 11377.6 nuORFdb_human ENST00000477091.5_1_21:35445925-35497723:+ ENST00000477091.5_1_21:35445925-35497723:+|ENST00000381151.4_2_21:35475142-35475211:+ MRPS6 3' Overlap dORF 3' Overlap dORF GN=MRPS6 3' Overlap dORF E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep4_Slot2-5_1_3522 5mio-rep1 E_20221201_NO30_400nL_HLAc1_5e6_directIP_titration_rep1_Slot1-37_1_3502.12472.1439.2 2 3 1 15.53 7.57 6.16 7.96 87.3 7 1 1 19500.0 Hydrogen 56 (Y) 9 SEFDNRTSW (Q) 9 TDCLSSSVSW S E/F|D|N\R\T|S\W 77.8 7 6 4 3 0 dual b/y bias 43.082516 0.85194 571.2454 1141.491 -0.0082 -7.1 -0.09999999999999964 4.37 11377.6 nuORFdb_human ENST00000477091.5_1_21:35445925-35497723:+ ENST00000477091.5_1_21:35445925-35497723:+|ENST00000381151.4_2_21:35475142-35475211:+ MRPS6 3' Overlap dORF 3' Overlap dORF GN=MRPS6 3' Overlap dORF E_20221201_NO30_400nL_HLAc1_5e6_directIP_titration_rep1_Slot1-37_1_3502 5mio-rep2 E_20221201_NO30_400nL_HLAc1_5e6_directIP_titration_rep2_Slot2-2_1_3510.12402.1432.2 2 1 1 9.01 5.21 4.08 3.79 73.5 7 1 1 5360.0 Hydrogen 56 (Y) 9 SEFDNRTSW (Q) 9 SESSSGPAPHGE S/E/F/D/N R/T\S\W 77.8 7 2 5 0 0 y-ion bias 42.83633 0.856386 571.2447 1141.491 -0.0082 -7.1 1.5999999999999996 4.37 11377.6 nuORFdb_human ENST00000477091.5_1_21:35445925-35497723:+ ENST00000477091.5_1_21:35445925-35497723:+|ENST00000381151.4_2_21:35475142-35475211:+ MRPS6 3' Overlap dORF 3' Overlap dORF GN=MRPS6 3' Overlap dORF E_20221201_NO30_400nL_HLAc1_5e6_directIP_titration_rep2_Slot2-2_1_3510 5mio-rep3 E_20221201_NO30_400nL_HLAc1_5e6_directIP_titration_rep3_Slot2-2_1_3511.12426.1440.2 2 1 1 13.56 6.46 5.38 7.1 84.3 8 1 1 4530.0 Hydrogen 56 (Y) 9 SEFDNRTSW (Q) 9 DFTGINCVDT S/E/F|D|N-R/T|S\W 100.0 9 4 6 3 1 dual b/y bias 42.926853 0.851215 571.2429 1141.491 -0.0122 -10.6 -2.4000000000000004 4.37 11377.6 nuORFdb_human ENST00000477091.5_1_21:35445925-35497723:+ ENST00000477091.5_1_21:35445925-35497723:+|ENST00000381151.4_2_21:35475142-35475211:+ MRPS6 3' Overlap dORF 3' Overlap dORF GN=MRPS6 3' Overlap dORF E_20221201_NO30_400nL_HLAc1_5e6_directIP_titration_rep3_Slot2-2_1_3511 10mio-rep1 E_20221201_NO30_400nL_HLAc1_1e7_directIP_titration_rep1_Slot2-3_1_3512.21528.612.1 1 2 1 9.47 4.43 6.81 5.04 73.0 7 0 0 4340.0 Hydrogen 42 (S) 9 SEFSGLGGI (-) 9 TDGSFLNL S E/F/S/G|L\G\G\I 77.8 7 4 4 1 0 unbiased 74.363045 1.359044 866.4189 866.425 -0.0064 -7.4 0.2999999999999998 4.0 5205.8 nuORFdb_human ENST00000566442.5_2_15:42565903-42566053:+ ENST00000566442.5_2_15:42565903-42566053:+ GANC 5' uORF 5' uORF GN=GANC 5' uORF E_20221201_NO30_400nL_HLAc1_1e7_directIP_titration_rep1_Slot2-3_1_3512 10mio-rep2 E_20221201_NO30_400nL_HLAc1_1e7_directIP_titration_rep2_Slot2-3_1_3513.21451.607.1 1 2 1 9.97 5.5 3.97 4.47 69.0 7 0 0 4110.0 Hydrogen 42 (S) 9 SEFSGLGGI (-) 9 GESFSLNL S E/F\S/G|L|G\G\I 77.8 7 5 4 2 0 unbiased 74.094612 1.362179 866.4196 866.425 -0.0054 -6.3 0.40000000000000036 4.0 5205.8 nuORFdb_human ENST00000566442.5_2_15:42565903-42566053:+ ENST00000566442.5_2_15:42565903-42566053:+ GANC 5' uORF 5' uORF GN=GANC 5' uORF E_20221201_NO30_400nL_HLAc1_1e7_directIP_titration_rep2_Slot2-3_1_3513 10mio-rep3 E_20221201_NO30_400nL_HLAc1_1e7_directIP_titration_rep3_Slot2-3_1_3514.21407.605.1 1 2 1 12.28 6.48 6.52 5.8 77.0 7 0 0 4140.0 Hydrogen 42 (S) 9 SEFSGLGGI (-) 9 nTFSGIVQ S E/F|S|G|L|G\G\I 77.8 7 6 5 4 0 dual b/y bias 73.944473 1.363188 866.4207 866.425 -0.0044 -5.1 1.2000000000000002 4.0 5205.8 nuORFdb_human ENST00000566442.5_2_15:42565903-42566053:+ ENST00000566442.5_2_15:42565903-42566053:+ GANC 5' uORF 5' uORF GN=GANC 5' uORF E_20221201_NO30_400nL_HLAc1_1e7_directIP_titration_rep3_Slot2-3_1_3514 20mio-rep1 E_20221201_NO30_400nL_HLAc1_2e7_directIP_titration_rep1_Slot2-4_1_3515.21405.607.1 1 1 1 11.2 5.8 7.09 5.4 76.6 7 0 0 2560.0 Hydrogen 42 (S) 9 SEFSGLGGI (-) 9 nTFSGIVQ S E/F|S|G\L\G\G\I 77.8 7 6 3 2 0 b-ion bias 73.9189 1.362043 866.4171 866.425 -0.0084 -9.7 -3.499999999999999 4.0 5205.8 nuORFdb_human ENST00000566442.5_2_15:42565903-42566053:+ ENST00000566442.5_2_15:42565903-42566053:+ GANC 5' uORF 5' uORF GN=GANC 5' uORF E_20221201_NO30_400nL_HLAc1_2e7_directIP_titration_rep1_Slot2-4_1_3515 20mio-rep2 E_20221201_NO30_400nL_HLAc1_2e7_directIP_titration_rep3_Slot2-4_1_3518.21459.595.1 1 2 1 11.92 4.87 8.08 7.05 78.3 7 0 0 4600.0 Hydrogen 42 (S) 9 SEFSGLGGI (-) 9 nTFSGIVQ S E/F|S/G|L\G\G\I 77.8 7 5 4 2 0 unbiased 74.122421 1.369216 866.4174 866.425 -0.0084 -9.7 -3.3999999999999995 4.0 5205.8 nuORFdb_human ENST00000566442.5_2_15:42565903-42566053:+ ENST00000566442.5_2_15:42565903-42566053:+ GANC 5' uORF 5' uORF GN=GANC 5' uORF E_20221201_NO30_400nL_HLAc1_2e7_directIP_titration_rep3_Slot2-4_1_3518 20mio-rep3 E_20221201_NO30_400nL_HLAc1_2e7_directIP_titration_rep4_Slot2-4_1_3519.21467.593.1 1 4 1 11.26 5.78 6.81 5.49 76.7 7 0 0 10100.0 Hydrogen 42 (S) 9 SEFSGLGGI (-) 9 nTFSGIVQ S E/F|S|G|L\G\G\I 77.8 7 6 4 3 0 dual b/y bias 74.142227 1.370337 866.4224 866.425 -0.0034 -4.0 1.7999999999999998 4.0 5205.8 nuORFdb_human ENST00000566442.5_2_15:42565903-42566053:+ ENST00000566442.5_2_15:42565903-42566053:+ GANC 5' uORF 5' uORF GN=GANC 5' uORF E_20221201_NO30_400nL_HLAc1_2e7_directIP_titration_rep4_Slot2-4_1_3519 40mio-rep1 E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep1_Slot1-04_1_3496.21675.577.1 1 2 1 7.83 3.11 5.86 4.72 63.3 7 0 0 9870.0 Hydrogen 42 (S) 9 SEFSGLGGI (-) 9 LYGTGEGGL S E/F/S/G\L\G\G\I 77.8 7 4 3 0 0 unbiased 74.876457 1.380096 866.4253 866.425 -0.0004 -0.5 2.7 4.0 5205.8 nuORFdb_human ENST00000566442.5_2_15:42565903-42566053:+ ENST00000566442.5_2_15:42565903-42566053:+ GANC 5' uORF 5' uORF GN=GANC 5' uORF E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep1_Slot1-04_1_3496 40mio-rep2 E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep2_Slot2-5_1_3520.21411.590.1 1 2 1 8.54 3.92 4.41 4.61 66.6 7 0 0 9390.0 Hydrogen 42 (S) 9 SEFSGLGGI (-) 9 nTFSGIVQ S E/F/S/G|L\G\G\I 77.8 7 4 4 1 0 unbiased 73.966354 1.372455 866.4219 866.425 -0.0034 -4.0 0.9000000000000004 4.0 5205.8 nuORFdb_human ENST00000566442.5_2_15:42565903-42566053:+ ENST00000566442.5_2_15:42565903-42566053:+ GANC 5' uORF 5' uORF GN=GANC 5' uORF E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep2_Slot2-5_1_3520 40mio-rep3 E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep3_Slot2-5_1_3521.21377.596.1 1 1 1 10.06 4.18 6.69 5.88 70.7 7 0 0 5920.0 Hydrogen 42 (S) 9 SEFSGLGGI (-) 9 nTFSGIVQ S E/F/S|G|L\G\G\I 77.8 7 5 4 2 0 unbiased 73.846016 1.368705 866.4242 866.425 -0.0014 -1.6 2.9999999999999996 4.0 5205.8 nuORFdb_human ENST00000566442.5_2_15:42565903-42566053:+ ENST00000566442.5_2_15:42565903-42566053:+ GANC 5' uORF 5' uORF GN=GANC 5' uORF E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep3_Slot2-5_1_3521 40mio-rep4 E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep4_Slot2-5_1_3522.21360.589.1 1 2 1 7.19 1.79 6.86 5.4 61.5 7 0 0 10000.0 Hydrogen 42 (S) 9 SEFSGLGGI (-) 9 nTFSGIVQ S E/F|S/G|L\G\G\I 77.8 7 5 4 2 0 unbiased 73.770317 1.372759 866.4224 866.425 -0.0034 -4.0 0.7000000000000002 4.0 5205.8 nuORFdb_human ENST00000566442.5_2_15:42565903-42566053:+ ENST00000566442.5_2_15:42565903-42566053:+ GANC 5' uORF 5' uORF GN=GANC 5' uORF E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep4_Slot2-5_1_3522 40mio-rep2 E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep2_Slot2-5_1_3520.17243.1336.2 2 1 1 9.45 1.3 6.89 8.14 59.8 8 1 1 5860.0 Hydrogen 70 (P) 11 SEGDFRTGVTW (L) 11 TDNGIVWYTW S E/G D/F|R|T/G\V|T\W 63.6 7 5 6 3 0 unbiased 59.562691 0.916512 627.7862 1254.575 -0.0102 -8.1 -3.1999999999999993 4.37 9512.5 nuORFdb_human ENST00000502489.5_1_5:31855042-31983301:+ ENST00000502489.5_1_5:31855042-31983301:+ PDZD2 ncRNA Retained Intron lncRNA GN=PDZD2 ncRNA Retained Intron E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep2_Slot2-5_1_3520 40mio-rep4 E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep4_Slot2-5_1_3522.17249.1343.2 2 1 1 7.07 2.18 4.99 4.89 59.7 6 1 1 5280.0 Hydrogen 70 (P) 11 SEGDFRTGVTW (L) 11 TDNGIVWYTW S E/G D\F/R/T G V|T\W 36.4 4 3 4 1 0 unbiased 59.568954 0.911888 627.7911 1254.575 -0.0002 -0.2 4.5 4.37 9512.5 nuORFdb_human ENST00000502489.5_1_5:31855042-31983301:+ ENST00000502489.5_1_5:31855042-31983301:+ PDZD2 ncRNA Retained Intron lncRNA GN=PDZD2 ncRNA Retained Intron E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep4_Slot2-5_1_3522 10mio-rep2 E_20221201_NO30_400nL_HLAc1_1e7_directIP_titration_rep2_Slot2-3_1_3513.8865.1543.2 2 3 1 8.7 6.03 3.84 2.68 62.6 8 1 1 11900.0 Hydrogen 36 (A) 9 SEGGQSLRI (-) 9 SENQSLLR S/E/G/G/Q/S\L/R\I 100.0 9 2 6 0 0 y-ion bias 30.628857 0.786205 473.7488 946.495 -0.0045 -4.8 1.9000000000000004 5.72 4826.3 nuORFdb_human ENST00000494751.5_1_6:33385543-33385820:- ENST00000494751.5_1_6:33385543-33385820:-|ENST00000465956.1_1_6:33385543-33385645:- CUTA 5' uORF 5' uORF GN=CUTA 5' uORF E_20221201_NO30_400nL_HLAc1_1e7_directIP_titration_rep2_Slot2-3_1_3513 20mio-rep1 E_20221201_NO30_400nL_HLAc1_2e7_directIP_titration_rep1_Slot2-4_1_3515.8804.1544.2 2 2 1 11.6 9.51 3.77 2.08 81.6 7 1 1 16500.0 Hydrogen 36 (A) 9 SEGGQSLRI (-) 9 SEGGQLRSI S E|G|G/Q|S/L/R\I 77.8 7 4 6 3 0 dual b/y bias 30.412615 0.785865 473.7482 946.495 -0.0065 -6.9 -0.7000000000000002 5.72 4826.3 nuORFdb_human ENST00000494751.5_1_6:33385543-33385820:- ENST00000494751.5_1_6:33385543-33385820:-|ENST00000465956.1_1_6:33385543-33385645:- CUTA 5' uORF 5' uORF GN=CUTA 5' uORF E_20221201_NO30_400nL_HLAc1_2e7_directIP_titration_rep1_Slot2-4_1_3515 20mio-rep2 E_20221201_NO30_400nL_HLAc1_2e7_directIP_titration_rep3_Slot2-4_1_3518.8985.1539.2 2 2 1 11.23 8.67 3.85 2.56 80.0 8 1 1 11400.0 Hydrogen 36 (A) 9 SEGGQSLRI (-) 9 SEnDVIRL S/E|G/G/Q|S/L|R\I 100.0 9 4 7 3 0 dual b/y bias 31.038456 0.78897 473.7485 946.495 -0.0065 -6.9 -0.6000000000000005 5.72 4826.3 nuORFdb_human ENST00000494751.5_1_6:33385543-33385820:- ENST00000494751.5_1_6:33385543-33385820:-|ENST00000465956.1_1_6:33385543-33385645:- CUTA 5' uORF 5' uORF GN=CUTA 5' uORF E_20221201_NO30_400nL_HLAc1_2e7_directIP_titration_rep3_Slot2-4_1_3518 20mio-rep3 E_20221201_NO30_400nL_HLAc1_2e7_directIP_titration_rep4_Slot2-4_1_3519.8837.1538.2 2 2 1 9.07 6.94 3.05 2.13 75.8 7 1 1 14500.0 Hydrogen 36 (A) 9 SEGGQSLRI (-) 9 SEGGQLRSI S E/G/G/Q|S/L/R\I 77.8 7 2 6 1 0 y-ion bias 30.529667 0.789691 473.7475 946.495 -0.0065 -6.9 -1.1000000000000005 5.72 4826.3 nuORFdb_human ENST00000494751.5_1_6:33385543-33385820:- ENST00000494751.5_1_6:33385543-33385820:-|ENST00000465956.1_1_6:33385543-33385645:- CUTA 5' uORF 5' uORF GN=CUTA 5' uORF E_20221201_NO30_400nL_HLAc1_2e7_directIP_titration_rep4_Slot2-4_1_3519 40mio-rep1 E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep1_Slot1-04_1_3496.9089.1518.2 2 1 1 10.89 8.63 4.46 2.26 76.2 8 1 1 23800.0 Hydrogen 36 (A) 9 SEGGQSLRI (-) 9 SENQSLLR S/E/G/G/Q|S/L|R\I 100.0 9 3 7 2 0 y-ion bias 31.400459 0.802092 473.7493 946.495 -0.0045 -4.8 -1.5999999999999996 5.72 4826.3 nuORFdb_human ENST00000494751.5_1_6:33385543-33385820:- ENST00000494751.5_1_6:33385543-33385820:-|ENST00000465956.1_1_6:33385543-33385645:- CUTA 5' uORF 5' uORF GN=CUTA 5' uORF E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep1_Slot1-04_1_3496 40mio-rep2 E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep2_Slot2-5_1_3520.8803.1524.2 2 1 1 10.48 7.76 3.4 2.71 65.9 6 1 1 17000.0 Hydrogen 36 (A) 9 SEGGQSLRI (-) 9 SENQSLLR S/E|G/G|Q|S-L R\I 77.8 7 4 5 3 1 dual b/y bias 30.412533 0.798092 473.7492 946.495 -0.0045 -4.8 0.10000000000000053 5.72 4826.3 nuORFdb_human ENST00000494751.5_1_6:33385543-33385820:- ENST00000494751.5_1_6:33385543-33385820:-|ENST00000465956.1_1_6:33385543-33385645:- CUTA 5' uORF 5' uORF GN=CUTA 5' uORF E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep2_Slot2-5_1_3520 40mio-rep3 E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep3_Slot2-5_1_3521.8810.1530.2 2 1 1 11.86 8.74 5.5 3.12 62.8 8 1 1 7600.0 Hydrogen 36 (A) 9 SEGGQSLRI (-) 9 SEGGQLRSI S/E/G|G/Q|S|L/R\I 100.0 9 4 7 3 0 dual b/y bias 30.43531 0.794725 473.747 946.495 -0.0085 -9.0 -4.4 5.72 4826.3 nuORFdb_human ENST00000494751.5_1_6:33385543-33385820:- ENST00000494751.5_1_6:33385543-33385820:-|ENST00000465956.1_1_6:33385543-33385645:- CUTA 5' uORF 5' uORF GN=CUTA 5' uORF E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep3_Slot2-5_1_3521 40mio-rep4 E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep4_Slot2-5_1_3522.8820.1527.2 2 1 1 13.95 10.8 4.74 3.15 72.3 8 1 1 8130.0 Hydrogen 36 (A) 9 SEGGQSLRI (-) 9 SEGGQLRSI S/E|G/G/Q|S/L|R\I 100.0 9 4 7 3 0 dual b/y bias 30.463461 0.796198 473.7477 946.495 -0.0065 -6.9 -2.2 5.72 4826.3 nuORFdb_human ENST00000494751.5_1_6:33385543-33385820:- ENST00000494751.5_1_6:33385543-33385820:-|ENST00000465956.1_1_6:33385543-33385645:- CUTA 5' uORF 5' uORF GN=CUTA 5' uORF E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep4_Slot2-5_1_3522 20mio-rep3 E_20221201_NO30_400nL_HLAc1_2e7_directIP_titration_rep4_Slot2-4_1_3519.14976.1499.2 2 2 1 7.74 2.59 5.15 5.15 60.9 6 1 1 12900.0 Hydrogen 24 (F) 9 SEGPTPLKF (S) 9 ESTGPKPLF S E/G/P/T/P L/K\F 55.6 5 1 5 0 0 y-ion bias 51.730125 0.814285 488.2577 975.515 -0.0058 -6.0 -0.20000000000000018 5.72 5581.6 nuORFdb_human ENST00000370763.5_1_1:78478758-78478905:+ ENST00000370763.5_1_1:78478758-78478905:+ DNAJB4 Out-of-Frame Out-of-Frame GN=DNAJB4 Out-of-Frame E_20221201_NO30_400nL_HLAc1_2e7_directIP_titration_rep4_Slot2-4_1_3519 10mio-rep1 E_20221201_NO30_400nL_HLAc1_1e7_directIP_titration_rep1_Slot2-3_1_3512.15970.524.1 1 2 1 12.57 8.88 1.71 3.68 89.2 7 0 0 5420.0 Hydrogen 21 (N) 9 SEIPTTSSL (R) 9 VTEGSLESL S E/I|P|T|T\S\S\L 77.8 7 6 4 3 0 dual b/y bias 55.168964 1.411523 934.4633 934.473 -0.0098 -10.5 -2.8 4.0 3667.0 smORF_v4|nuORFdb_human DAXX_6:33290641-33289756:+ DAXX_6:33290641-33289756:+|ENST00000266000.10_2_6:33289711-33290733:-|ENST00000374542.9_2_6:33289711-33290733:-|ENST00000453407.6_3_6:33289711-33291800:-|ENST00000446511.1_1_6:33289711-33290545:- DAXX uORF 6:33290641-33289756:+ 6 DAXX 5' uORF 5' uORF GN=DAXX E_20221201_NO30_400nL_HLAc1_1e7_directIP_titration_rep1_Slot2-3_1_3512 10mio-rep2 E_20221201_NO30_400nL_HLAc1_1e7_directIP_titration_rep2_Slot2-3_1_3513.15929.568.1 1 3 1 11.97 9.35 1.7 2.62 89.1 6 0 0 16000.0 Hydrogen 21 (N) 9 SEIPTTSSL (R) 9 ESLADLSSL S E I|P\T|T\S\S\L 66.7 6 6 2 2 0 b-ion bias 55.02383 1.385308 934.469 934.473 -0.0038 -4.0 2.7 4.0 3667.0 smORF_v4|nuORFdb_human DAXX_6:33290641-33289756:+ DAXX_6:33290641-33289756:+|ENST00000266000.10_2_6:33289711-33290733:-|ENST00000374542.9_2_6:33289711-33290733:-|ENST00000453407.6_3_6:33289711-33291800:-|ENST00000446511.1_1_6:33289711-33290545:- DAXX uORF 6:33290641-33289756:+ 6 DAXX 5' uORF 5' uORF GN=DAXX E_20221201_NO30_400nL_HLAc1_1e7_directIP_titration_rep2_Slot2-3_1_3513 10mio-rep3 E_20221201_NO30_400nL_HLAc1_1e7_directIP_titration_rep3_Slot2-3_1_3514.15878.524.1 1 1 1 12.02 9.52 2.15 2.5 86.2 6 0 0 4860.0 Hydrogen 21 (N) 9 SEIPTTSSL (R) 9 VTEGSLESL S E I|P/T|T\S\S\L 66.7 6 5 3 2 0 unbiased 54.850418 1.411943 934.469 934.473 -0.0038 -4.0 2.3 4.0 3667.0 smORF_v4|nuORFdb_human DAXX_6:33290641-33289756:+ DAXX_6:33290641-33289756:+|ENST00000266000.10_2_6:33289711-33290733:-|ENST00000374542.9_2_6:33289711-33290733:-|ENST00000453407.6_3_6:33289711-33291800:-|ENST00000446511.1_1_6:33289711-33290545:- DAXX uORF 6:33290641-33289756:+ 6 DAXX 5' uORF 5' uORF GN=DAXX E_20221201_NO30_400nL_HLAc1_1e7_directIP_titration_rep3_Slot2-3_1_3514 20mio-rep2 E_20221201_NO30_400nL_HLAc1_2e7_directIP_titration_rep3_Slot2-4_1_3518.15952.516.1 1 2 1 12.07 9.5 2.2 2.57 89.2 6 0 0 25400.0 Hydrogen 21 (N) 9 SEIPTTSSL (R) 9 ESLADLSSL S E/I|P T|T\S\S\L 55.6 5 5 3 2 0 unbiased 55.098164 1.41636 934.4662 934.473 -0.0068 -7.2 -0.9000000000000004 4.0 3667.0 smORF_v4|nuORFdb_human DAXX_6:33290641-33289756:+ DAXX_6:33290641-33289756:+|ENST00000266000.10_2_6:33289711-33290733:-|ENST00000374542.9_2_6:33289711-33290733:-|ENST00000453407.6_3_6:33289711-33291800:-|ENST00000446511.1_1_6:33289711-33290545:- DAXX uORF 6:33290641-33289756:+ 6 DAXX 5' uORF 5' uORF GN=DAXX E_20221201_NO30_400nL_HLAc1_2e7_directIP_titration_rep3_Slot2-4_1_3518 20mio-rep3 E_20221201_NO30_400nL_HLAc1_2e7_directIP_titration_rep4_Slot2-4_1_3519.15889.514.1 1 3 1 13.19 10.62 2.33 2.56 90.9 7 0 0 16800.0 Hydrogen 21 (N) 9 SEIPTTSSL (R) 9 ESLADLSSL S E/I/P|T|T\S\S\L 77.8 7 5 4 2 0 unbiased 54.88129 1.417581 934.4665 934.473 -0.0068 -7.2 -1.4000000000000004 4.0 3667.0 smORF_v4|nuORFdb_human DAXX_6:33290641-33289756:+ DAXX_6:33290641-33289756:+|ENST00000266000.10_2_6:33289711-33290733:-|ENST00000374542.9_2_6:33289711-33290733:-|ENST00000453407.6_3_6:33289711-33291800:-|ENST00000446511.1_1_6:33289711-33290545:- DAXX uORF 6:33290641-33289756:+ 6 DAXX 5' uORF 5' uORF GN=DAXX E_20221201_NO30_400nL_HLAc1_2e7_directIP_titration_rep4_Slot2-4_1_3519 40mio-rep2 E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep2_Slot2-5_1_3520.15842.514.1 1 4 1 13.56 11.0 2.31 2.56 89.4 7 0 0 64400.0 Hydrogen 21 (N) 9 SEIPTTSSL (R) 9 ESLADLSSL S E/I|P|T|T\S\S\L 77.8 7 6 4 3 0 dual b/y bias 54.7243 1.417602 934.4683 934.473 -0.0048 -5.1 -0.1999999999999993 4.0 3667.0 smORF_v4|nuORFdb_human DAXX_6:33290641-33289756:+ DAXX_6:33290641-33289756:+|ENST00000266000.10_2_6:33289711-33290733:-|ENST00000374542.9_2_6:33289711-33290733:-|ENST00000453407.6_3_6:33289711-33291800:-|ENST00000446511.1_1_6:33289711-33290545:- DAXX uORF 6:33290641-33289756:+ 6 DAXX 5' uORF 5' uORF GN=DAXX E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep2_Slot2-5_1_3520 40mio-rep3 E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep3_Slot2-5_1_3521.15853.514.1 1 2 1 14.07 10.44 1.64 3.63 89.5 7 0 0 63100.0 Hydrogen 21 (N) 9 SEIPTTSSL (R) 9 ESLADLSSL S E/I|P|T|T\S\S\L 77.8 7 6 4 3 0 dual b/y bias 54.763474 1.417924 934.4701 934.473 -0.0028 -3.0 1.5999999999999996 4.0 3667.0 smORF_v4|nuORFdb_human DAXX_6:33290641-33289756:+ DAXX_6:33290641-33289756:+|ENST00000266000.10_2_6:33289711-33290733:-|ENST00000374542.9_2_6:33289711-33290733:-|ENST00000453407.6_3_6:33289711-33291800:-|ENST00000446511.1_1_6:33289711-33290545:- DAXX uORF 6:33290641-33289756:+ 6 DAXX 5' uORF 5' uORF GN=DAXX E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep3_Slot2-5_1_3521 40mio-rep4 E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep4_Slot2-5_1_3522.15842.511.1 1 2 1 12.01 10.43 1.97 1.58 87.8 6 0 0 66900.0 Hydrogen 21 (N) 9 SEIPTTSSL (R) 9 ESLADLSSL S E/I|P T|T\S\S\L 55.6 5 5 3 2 0 unbiased 54.710751 1.419471 934.468 934.473 -0.0048 -5.1 -0.39999999999999947 4.0 3667.0 smORF_v4|nuORFdb_human DAXX_6:33290641-33289756:+ DAXX_6:33290641-33289756:+|ENST00000266000.10_2_6:33289711-33290733:-|ENST00000374542.9_2_6:33289711-33290733:-|ENST00000453407.6_3_6:33289711-33291800:-|ENST00000446511.1_1_6:33289711-33290545:- DAXX uORF 6:33290641-33289756:+ 6 DAXX 5' uORF 5' uORF GN=DAXX E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep4_Slot2-5_1_3522 5mio-rep1 E_20221201_NO30_400nL_HLAc1_5e6_directIP_titration_rep1_Slot1-37_1_3502.16223.514.1 1 1 1 12.2 10.25 1.7 1.95 90.0 6 0 0 2800.0 Hydrogen 21 (N) 9 SEIPTTSSL (R) 9 VTEGSLESL S E I|P\T|T\S\S\L 66.7 6 6 2 2 0 b-ion bias 56.038803 1.417733 934.4663 934.473 -0.0068 -7.2 -0.20000000000000018 4.0 3667.0 smORF_v4|nuORFdb_human DAXX_6:33290641-33289756:+ DAXX_6:33290641-33289756:+|ENST00000266000.10_2_6:33289711-33290733:-|ENST00000374542.9_2_6:33289711-33290733:-|ENST00000453407.6_3_6:33289711-33291800:-|ENST00000446511.1_1_6:33289711-33290545:- DAXX uORF 6:33290641-33289756:+ 6 DAXX 5' uORF 5' uORF GN=DAXX E_20221201_NO30_400nL_HLAc1_5e6_directIP_titration_rep1_Slot1-37_1_3502 5mio-rep2 E_20221201_NO30_400nL_HLAc1_5e6_directIP_titration_rep2_Slot2-2_1_3510.16122.531.1 1 1 1 12.51 8.88 1.67 3.63 86.1 7 0 0 3480.0 Hydrogen 21 (N) 9 SEIPTTSSL (R) 9 VTEGSLESL S E/I|P|T|T\S\S\L 77.8 7 6 4 3 0 dual b/y bias 55.685162 1.407217 934.4634 934.473 -0.0098 -10.5 -1.8000000000000007 4.0 3667.0 smORF_v4|nuORFdb_human DAXX_6:33290641-33289756:+ DAXX_6:33290641-33289756:+|ENST00000266000.10_2_6:33289711-33290733:-|ENST00000374542.9_2_6:33289711-33290733:-|ENST00000453407.6_3_6:33289711-33291800:-|ENST00000446511.1_1_6:33289711-33290545:- DAXX uORF 6:33290641-33289756:+ 6 DAXX 5' uORF 5' uORF GN=DAXX E_20221201_NO30_400nL_HLAc1_5e6_directIP_titration_rep2_Slot2-2_1_3510 40mio-rep1 E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep1_Slot1-04_1_3496.20725.1485.2 2 3 1 12.46 5.69 5.87 6.77 80.4 7 1 1 17900.0 Hydrogen 21 (R) 9 SELGIRVGF (-) 9 SELLGGFRV S E|L|G|I/R\V\G\F 77.8 7 6 4 3 0 dual b/y bias 71.594734 0.822793 489.2712 977.542 -0.0067 -6.9 -3.7 5.72 3105.5 nuORFdb_human ENST00000381401.10_1_X:1511360-1511447:- ENST00000381401.10_1_X:1511360-1511447:- SLC25A6 5' uORF 5' uORF GN=SLC25A6 5' uORF E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep1_Slot1-04_1_3496 40mio-rep2 E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep2_Slot2-5_1_3520.20527.1494.2 2 3 1 13.47 8.77 5.45 4.7 82.3 7 1 1 15300.0 Hydrogen 21 (R) 9 SELGIRVGF (-) 9 TDILVNGVF S E|L|G|I|R|V\G\F 77.8 7 7 5 5 0 dual b/y bias 70.913078 0.817501 489.2715 977.542 -0.0067 -6.9 -2.0 5.72 3105.5 nuORFdb_human ENST00000381401.10_1_X:1511360-1511447:- ENST00000381401.10_1_X:1511360-1511447:- SLC25A6 5' uORF 5' uORF GN=SLC25A6 5' uORF E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep2_Slot2-5_1_3520 40mio-rep1 E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep1_Slot1-04_1_3496.24649.361.1 1 1 1 8.13 5.33 3.17 2.8 72.6 6 0 0 4130.0 Hydrogen 56 (V) 9 SENSLDILM (H) 9 TnNSLVELM S E N\S\L\D\I\L\M 66.7 6 6 0 0 0 b-ion bias 85.150093 1.508629 1021.4806 1021.487 -0.007 -6.9 -3.7 3.66 8738.8 nuORFdb_human ENST00000591899.7_1_19:1598028-1599464:- ENST00000591899.7_1_19:1598028-1599464:- UQCR11 3' dORF 3' dORF GN=UQCR11 3' dORF E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep1_Slot1-04_1_3496 40mio-rep2 E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep2_Slot2-5_1_3520.11718.1488.2 2 1 1 9.15 3.06 5.59 6.1 60.7 7 0 0 12400.0 Hydrogen 58 (G) 9 SESTTHLAF (H) 9 TnANPSQLF S/E/S/T/T H\L\A\F 77.8 7 3 4 0 0 unbiased 40.479561 0.821323 496.7357 992.468 -0.0036 -3.7 1.2000000000000002 5.22 8603.6 nuORFdb_human ENST00000257663.3_1_7:77423607-77427674:- ENST00000257663.3_1_7:77423607-77427674:- TMEM60 5' Overlap uORF 5' Overlap uORF GN=TMEM60 5' Overlap uORF E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep2_Slot2-5_1_3520 40mio-rep4 E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep4_Slot2-5_1_3522.11720.1493.2 2 1 1 8.0 2.94 5.05 5.06 58.2 6 0 0 12200.0 Hydrogen 58 (G) 9 SESTTHLAF (H) 9 TnANPSQLF S E/S/T/T H\L\A\F 55.6 5 3 3 0 0 unbiased 40.474434 0.818194 496.7351 992.468 -0.0056 -5.7 -1.0 5.22 8603.6 nuORFdb_human ENST00000257663.3_1_7:77423607-77427674:- ENST00000257663.3_1_7:77423607-77427674:- TMEM60 5' Overlap uORF 5' Overlap uORF GN=TMEM60 5' Overlap uORF E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep4_Slot2-5_1_3522 10mio-rep1 E_20221201_NO30_400nL_HLAc1_1e7_directIP_titration_rep1_Slot2-3_1_3512.18393.437.1 1 1 1 10.29 5.69 4.43 4.6 76.7 5 0 0 3650.0 Hydrogen 56 (S) 9 SETPIVQNL (Q) 9 GLEEVVQNL S E/T|P I|V|Q\N L 33.3 3 4 4 3 0 dual b/y bias 63.536537 1.463408 1000.5232 1000.531 -0.011 -10.9 -3.2 4.0 12984.3 nuORFdb_human ENST00000573177.1_1_18:3603669-3604008:+ ENST00000573177.1_1_18:3603669-3604008:+ DLGAP1-AS2 Antisense lncRNA GN=DLGAP1-AS2 Antisense E_20221201_NO30_400nL_HLAc1_1e7_directIP_titration_rep1_Slot2-3_1_3512 10mio-rep2 E_20221201_NO30_400nL_HLAc1_1e7_directIP_titration_rep2_Slot2-3_1_3513.18326.435.1 1 3 1 11.52 5.32 7.07 6.2 79.1 7 0 0 7780.0 Hydrogen 56 (S) 9 SETPIVQNL (Q) 9 GLEEVVQNL S E/T/P\I|V|Q\N\L 77.8 7 5 4 2 0 unbiased 63.302578 1.464639 1000.5248 1000.531 -0.011 -10.9 -4.2 4.0 12984.3 nuORFdb_human ENST00000573177.1_1_18:3603669-3604008:+ ENST00000573177.1_1_18:3603669-3604008:+ DLGAP1-AS2 Antisense lncRNA GN=DLGAP1-AS2 Antisense E_20221201_NO30_400nL_HLAc1_1e7_directIP_titration_rep2_Slot2-3_1_3513 20mio-rep1 E_20221201_NO30_400nL_HLAc1_2e7_directIP_titration_rep1_Slot2-4_1_3515.18245.441.1 1 3 1 13.81 5.26 9.41 8.55 83.2 7 0 0 13100.0 Hydrogen 56 (S) 9 SETPIVQNL (Q) 9 SELPTVLGAN S E/T/P\I|V|Q\N\L 77.8 7 5 4 2 0 unbiased 63.010506 1.461038 1000.52 1000.531 -0.011 -10.9 -4.7 4.0 12984.3 nuORFdb_human ENST00000573177.1_1_18:3603669-3604008:+ ENST00000573177.1_1_18:3603669-3604008:+ DLGAP1-AS2 Antisense lncRNA GN=DLGAP1-AS2 Antisense E_20221201_NO30_400nL_HLAc1_2e7_directIP_titration_rep1_Slot2-4_1_3515 20mio-rep2 E_20221201_NO30_400nL_HLAc1_2e7_directIP_titration_rep3_Slot2-4_1_3518.18333.431.1 1 4 1 12.94 6.67 6.77 6.27 84.4 6 0 0 12500.0 Hydrogen 56 (S) 9 SETPIVQNL (Q) 9 GLEEVVQNL S E/T|P I|V|Q\N\L 55.6 5 5 4 3 0 dual b/y bias 63.323742 1.467228 1000.5196 1000.531 -0.011 -10.9 -4.6000000000000005 4.0 12984.3 nuORFdb_human ENST00000573177.1_1_18:3603669-3604008:+ ENST00000573177.1_1_18:3603669-3604008:+ DLGAP1-AS2 Antisense lncRNA GN=DLGAP1-AS2 Antisense E_20221201_NO30_400nL_HLAc1_2e7_directIP_titration_rep3_Slot2-4_1_3518 40mio-rep1 E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep1_Slot1-04_1_3496.18537.407.1 1 2 1 12.86 6.26 6.89 6.61 86.7 7 0 0 25600.0 Hydrogen 56 (S) 9 SETPIVQNL (Q) 9 GLEEVVQNL S E/T/P\I|V|Q\N\L 77.8 7 5 4 2 0 unbiased 64.03582 1.480905 1000.5267 1000.531 -0.001 -1.0 2.2 4.0 12984.3 nuORFdb_human ENST00000573177.1_1_18:3603669-3604008:+ ENST00000573177.1_1_18:3603669-3604008:+ DLGAP1-AS2 Antisense lncRNA GN=DLGAP1-AS2 Antisense E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep1_Slot1-04_1_3496 40mio-rep3 E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep3_Slot2-5_1_3521.18225.426.1 1 1 1 13.68 7.05 7.3 6.62 86.5 7 0 0 6250.0 Hydrogen 56 (S) 9 SETPIVQNL (Q) 9 TESLPNLGGI S E/T/P\I|V|Q\N\L 77.8 7 5 4 2 0 unbiased 62.957737 1.469884 1000.5279 1000.531 -0.001 -1.0 3.5999999999999996 4.0 12984.3 nuORFdb_human ENST00000573177.1_1_18:3603669-3604008:+ ENST00000573177.1_1_18:3603669-3604008:+ DLGAP1-AS2 Antisense lncRNA GN=DLGAP1-AS2 Antisense E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep3_Slot2-5_1_3521 40mio-rep4 E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep4_Slot2-5_1_3522.18217.423.1 1 2 1 12.13 8.21 5.05 3.91 79.9 5 0 0 13100.0 Hydrogen 56 (S) 9 SETPIVQNL (Q) 9 GLEEVVQNL S E/T/P I|V|Q\N L 33.3 3 3 4 2 0 unbiased 62.912476 1.471922 1000.5255 1000.531 -0.001 -1.0 3.7 4.0 12984.3 nuORFdb_human ENST00000573177.1_1_18:3603669-3604008:+ ENST00000573177.1_1_18:3603669-3604008:+ DLGAP1-AS2 Antisense lncRNA GN=DLGAP1-AS2 Antisense E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep4_Slot2-5_1_3522 5mio-rep1 E_20221201_NO30_400nL_HLAc1_5e6_directIP_titration_rep1_Slot1-37_1_3502.18635.427.1 1 1 1 10.74 6.14 4.51 4.6 76.9 6 0 0 1940.0 Hydrogen 56 (S) 9 SETPIVQNL (Q) 9 VEELGVAGNI S E/T/P I|V|Q\N\L 55.6 5 4 4 2 0 unbiased 64.36599 1.469132 1000.5225 1000.531 -0.011 -10.9 -3.9000000000000004 4.0 12984.3 nuORFdb_human ENST00000573177.1_1_18:3603669-3604008:+ ENST00000573177.1_1_18:3603669-3604008:+ DLGAP1-AS2 Antisense lncRNA GN=DLGAP1-AS2 Antisense E_20221201_NO30_400nL_HLAc1_5e6_directIP_titration_rep1_Slot1-37_1_3502 5mio-rep2 E_20221201_NO30_400nL_HLAc1_5e6_directIP_titration_rep2_Slot2-2_1_3510.18503.444.1 1 1 1 11.93 6.02 7.1 5.91 79.8 6 0 0 1480.0 Hydrogen 56 (S) 9 SETPIVQNL (Q) 9 TIPVSEQNI S E/T/P I|V|Q|N\L 55.6 5 4 5 3 0 dual b/y bias 63.908768 1.459393 1000.5231 1000.531 -0.011 -10.9 -2.200000000000001 4.0 12984.3 nuORFdb_human ENST00000573177.1_1_18:3603669-3604008:+ ENST00000573177.1_1_18:3603669-3604008:+ DLGAP1-AS2 Antisense lncRNA GN=DLGAP1-AS2 Antisense E_20221201_NO30_400nL_HLAc1_5e6_directIP_titration_rep2_Slot2-2_1_3510 40mio-rep1 E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep1_Slot1-04_1_3496.7041.1298.2 2 1 1 13.08 3.52 8.81 9.56 75.3 9 2 2 2790.0 Hydrogen 61 (S) 11 SEVAGVKSKDF (M) 11 SEVFELVSKE S/E/V|A/G V|K/S-K|D\F 81.8 9 4 7 3 1 unbiased 24.329538 0.940329 583.8029 1166.605 -0.0065 -5.5 -2.3 5.79 10146.1 nuORFdb_human ENST00000461310.5_1_10:70091580-70098360:+ ENST00000461310.5_1_10:70091580-70098360:+ HNRNPH3 5' Overlap uORF 5' Overlap uORF GN=HNRNPH3 5' Overlap uORF E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep1_Slot1-04_1_3496 40mio-rep2 E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep2_Slot2-5_1_3520.6743.1310.2 2 1 1 9.8 4.85 5.2 4.95 68.6 7 2 2 3190.0 Hydrogen 61 (S) 11 SEVAGVKSKDF (M) 11 SEVAVLTTFAE S E/V|A|G V/K/S K-D\F 45.5 5 3 5 2 1 unbiased 23.300421 0.932611 583.8046 1166.605 -0.0025 -2.1 2.8000000000000003 5.79 10146.1 nuORFdb_human ENST00000461310.5_1_10:70091580-70098360:+ ENST00000461310.5_1_10:70091580-70098360:+ HNRNPH3 5' Overlap uORF 5' Overlap uORF GN=HNRNPH3 5' Overlap uORF E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep2_Slot2-5_1_3520 40mio-rep4 E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep4_Slot2-5_1_3522.6763.1311.2 2 1 1 12.66 6.64 7.51 6.03 75.7 8 2 2 2830.0 Hydrogen 61 (S) 11 SEVAGVKSKDF (M) 11 SEVAVLTTFAE S E/V|A|G V|K/S\K\D\F 63.6 7 6 5 3 0 unbiased 23.361532 0.931916 583.8048 1166.605 -0.0025 -2.1 2.6 5.79 10146.1 nuORFdb_human ENST00000461310.5_1_10:70091580-70098360:+ ENST00000461310.5_1_10:70091580-70098360:+ HNRNPH3 5' Overlap uORF 5' Overlap uORF GN=HNRNPH3 5' Overlap uORF E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep4_Slot2-5_1_3522 SCP E_20221201_NO30_400nL_HLAc1_4e7_PDAC_Slot2-6_1_3524.30023.72.1 1 2 1 14.45 5.02 9.3 9.44 87.3 8 0 0 19400.0 Hydrogen 98 (T) 11 SFLPTSPLLGF (L) 11 IFSTPLSPFLG S F/L/P/T|S|P L|L\G-F 63.6 7 4 6 3 1 unbiased 103.727921 1.67742 1178.6417 1178.646 -0.0056 -4.7 -2.3000000000000003 5.24 15573.7 nuORFdb_human ENST00000409443.6_1_2:172016940-172017408:- ENST00000409443.6_1_2:172016940-172017408:- TLK1 5' uORF 5' uORF GN=TLK1 5' uORF E_20221201_NO30_400nL_HLAc1_4e7_PDAC_Slot2-6_1_3524 40mio-rep3 E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep3_Slot2-5_1_3521.14155.1496.2 2 1 1 8.59 6.73 1.26 1.86 66.7 5 1 1 7950.0 Hydrogen 49 (V) 9 SFVSRALPA (I) 9 SFPDVRKV S/F|V/S R A-L\P A 44.4 4 2 3 1 1 unbiased 48.900383 0.816268 474.2686 947.531 -0.0002 -0.2 4.3999999999999995 9.47 6705.7 nuORFdb_human ENST00000574202.1_1_17:3856610-3867516:- ENST00000574202.1_1_17:3856610-3867516:- ATP2A3 ncRNA Retained Intron lncRNA GN=ATP2A3 ncRNA Retained Intron E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep3_Slot2-5_1_3521 40mio-rep1 E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep1_Slot1-04_1_3496.16853.1536.2 2 1 1 8.1 4.36 3.71 3.74 67.3 6 1 1 4300.0 Hydrogen 50 (F) 9 SGTVRLAPF (-) 9 SLRSGLAFP S/G/T/V R L|A|P\F 66.7 6 3 5 2 0 unbiased 58.21487 0.791108 474.2659 947.531 -0.0062 -6.5 -3.3 9.47 6867.8 nuORFdb_human ENST00000467020.5_1_6:35436211-35436817:+ ENST00000467020.5_1_6:35436211-35436817:+ RPL10A ncRNA Processed Transcript lncRNA GN=RPL10A ncRNA Processed Transcript E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep1_Slot1-04_1_3496 10mio-rep1 E_20221201_NO30_400nL_HLAc1_1e7_directIP_titration_rep1_Slot2-3_1_3512.14282.1475.2 2 1 1 7.5 4.86 0.22 2.64 75.8 6 1 1 6570.0 Hydrogen 55 (R) 9 SGWGEPRSW (H) 9 SGWGGRAEAGGG S/G/W/G|E|P R S-W 66.7 6 2 5 2 1 y-ion bias 49.338566 0.829281 531.2387 1061.48 -0.0092 -8.7 -0.9999999999999991 5.72 9892.3 smORF_v4|nuORFdb_human EIF3J-AS1_15:44828599-44828859:- EIF3J-AS1_15:44828599-44828859:-|ENST00000313807.4_1_15:44828601-44828859:- EIF3J-AS1 lncRNA 15:44828599-44828859:- 15 EIF3J-AS1 lincRNA lncRNA GN=EIF3J-AS1 E_20221201_NO30_400nL_HLAc1_1e7_directIP_titration_rep1_Slot2-3_1_3512 10mio-rep2 E_20221201_NO30_400nL_HLAc1_1e7_directIP_titration_rep2_Slot2-3_1_3513.14251.1480.2 2 1 1 11.38 5.15 4.52 6.23 86.4 7 1 1 6590.0 Hydrogen 55 (R) 9 SGWGEPRSW (H) 9 SGWGGRAEAGGG S/G/W|G|E|P/R S-W 77.8 7 3 6 3 1 dual b/y bias 49.2313 0.826074 531.2397 1061.48 -0.0072 -6.8 -0.09999999999999964 5.72 9892.3 smORF_v4|nuORFdb_human EIF3J-AS1_15:44828599-44828859:- EIF3J-AS1_15:44828599-44828859:-|ENST00000313807.4_1_15:44828601-44828859:- EIF3J-AS1 lncRNA 15:44828599-44828859:- 15 EIF3J-AS1 lincRNA lncRNA GN=EIF3J-AS1 E_20221201_NO30_400nL_HLAc1_1e7_directIP_titration_rep2_Slot2-3_1_3513 20mio-rep3 E_20221201_NO30_400nL_HLAc1_2e7_directIP_titration_rep4_Slot2-4_1_3519.14191.1477.2 2 2 1 11.19 5.54 4.62 5.65 79.4 7 1 1 12800.0 Hydrogen 55 (R) 9 SGWGEPRSW (H) 9 SGWGGRAEAGGG S/G/W|G|E|P R-S\W 77.8 7 4 5 3 1 dual b/y bias 49.017204 0.827827 531.2401 1061.48 -0.0072 -6.8 -1.0 5.72 9892.3 smORF_v4|nuORFdb_human EIF3J-AS1_15:44828599-44828859:- EIF3J-AS1_15:44828599-44828859:-|ENST00000313807.4_1_15:44828601-44828859:- EIF3J-AS1 lncRNA 15:44828599-44828859:- 15 EIF3J-AS1 lincRNA lncRNA GN=EIF3J-AS1 E_20221201_NO30_400nL_HLAc1_2e7_directIP_titration_rep4_Slot2-4_1_3519 40mio-rep1 E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep1_Slot1-04_1_3496.14468.1447.2 2 1 1 13.5 7.45 4.5 6.04 94.0 6 1 1 24600.0 Hydrogen 55 (R) 9 SGWGEPRSW (H) 9 SGWGGRAEAGGG S/G/W|G|E|P R\S-W 77.8 7 4 5 3 1 dual b/y bias 49.977097 0.847033 531.2416 1061.48 -0.0032 -3.0 0.20000000000000018 5.72 9892.3 smORF_v4|nuORFdb_human EIF3J-AS1_15:44828599-44828859:- EIF3J-AS1_15:44828599-44828859:-|ENST00000313807.4_1_15:44828601-44828859:- EIF3J-AS1 lncRNA 15:44828599-44828859:- 15 EIF3J-AS1 lincRNA lncRNA GN=EIF3J-AS1 E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep1_Slot1-04_1_3496 40mio-rep2 E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep2_Slot2-5_1_3520.14148.1458.2 2 1 1 13.7 7.77 5.69 5.93 86.0 7 1 1 16000.0 Hydrogen 55 (R) 9 SGWGEPRSW (H) 9 SGEWGPRCL S/G/W|G|E|P R|S\W 77.8 7 5 6 4 0 dual b/y bias 48.873341 0.84014 531.2401 1061.48 -0.0072 -6.8 -1.8999999999999995 5.72 9892.3 smORF_v4|nuORFdb_human EIF3J-AS1_15:44828599-44828859:- EIF3J-AS1_15:44828599-44828859:-|ENST00000313807.4_1_15:44828601-44828859:- EIF3J-AS1 lncRNA 15:44828599-44828859:- 15 EIF3J-AS1 lincRNA lncRNA GN=EIF3J-AS1 E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep2_Slot2-5_1_3520 40mio-rep3 E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep3_Slot2-5_1_3521.14164.1464.2 2 1 1 12.05 6.12 5.93 5.93 84.3 7 1 1 8740.0 Hydrogen 55 (R) 9 SGWGEPRSW (H) 9 SGWGGRAEAGGG S/G/W|G|E|P/R\S-W 100.0 9 4 6 3 1 dual b/y bias 48.931435 0.836387 531.2409 1061.48 -0.0052 -4.9 -0.3000000000000007 5.72 9892.3 smORF_v4|nuORFdb_human EIF3J-AS1_15:44828599-44828859:- EIF3J-AS1_15:44828599-44828859:-|ENST00000313807.4_1_15:44828601-44828859:- EIF3J-AS1 lncRNA 15:44828599-44828859:- 15 EIF3J-AS1 lincRNA lncRNA GN=EIF3J-AS1 E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep3_Slot2-5_1_3521 40mio-rep4 E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep4_Slot2-5_1_3522.14138.1462.2 2 1 1 10.19 4.81 3.28 5.38 87.6 6 1 1 14500.0 Hydrogen 55 (R) 9 SGWGEPRSW (H) 9 SGWGGRAEAGGG S/G/W|G|E|P R\S-W 77.8 7 4 5 3 1 dual b/y bias 48.828056 0.837598 531.2409 1061.48 -0.0052 -4.9 -0.20000000000000018 5.72 9892.3 smORF_v4|nuORFdb_human EIF3J-AS1_15:44828599-44828859:- EIF3J-AS1_15:44828599-44828859:-|ENST00000313807.4_1_15:44828601-44828859:- EIF3J-AS1 lncRNA 15:44828599-44828859:- 15 EIF3J-AS1 lincRNA lncRNA GN=EIF3J-AS1 E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep4_Slot2-5_1_3522 5mio-rep1 E_20221201_NO30_400nL_HLAc1_5e6_directIP_titration_rep1_Slot1-37_1_3502.14536.1474.2 2 1 1 10.78 3.8 5.95 6.98 81.2 8 1 1 5200.0 Hydrogen 55 (R) 9 SGWGEPRSW (H) 9 SGWGGRAEAGGG S/G/W|G|E|P/R\S\W 100.0 9 5 6 3 0 dual b/y bias 50.211407 0.82974 531.2393 1061.48 -0.0092 -8.7 -1.6999999999999993 5.72 9892.3 smORF_v4|nuORFdb_human EIF3J-AS1_15:44828599-44828859:- EIF3J-AS1_15:44828599-44828859:-|ENST00000313807.4_1_15:44828601-44828859:- EIF3J-AS1 lncRNA 15:44828599-44828859:- 15 EIF3J-AS1 lincRNA lncRNA GN=EIF3J-AS1 E_20221201_NO30_400nL_HLAc1_5e6_directIP_titration_rep1_Slot1-37_1_3502 Eva-50mio-3fr AL20210827_EV_HLA_PDAC_PANFRO413_2D_50e6_W632_classI_fxn3.31820.31820.2 2 1 1 9.2 6.03 3.32 3.17 63.8 5 0 0 12000000.0 Hydrogen 13 (Y) 9 SHSLPVPFF (Y) 9 SIPFHSVPF S H S L\P\V\P\F/F 55.6 5 4 1 0 0 b-ion bias 0.954 94.9 58.47 100.0 0.0 515.7716 1030.536 0.0004 0.3 -0.2 6.46 3458.8 nuORFdb_human ENST00000393295.7_1_3:128880030-128880126:- ENST00000393295.7_1_3:128880030-128880126:- ISY1 5' uORF 5' uORF GN=ISY1 5' uORF AL20210827_EV_HLA_PDAC_PANFRO413_2D_50e6_W632_classI_fxn3 40mio-rep3 E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep3_Slot2-5_1_3521.28441.433.1 1 1 1 7.98 2.34 4.97 5.64 69.0 7 0 0 2200.0 Hydrogen 46 (T) 9 SIIPALWEA (E) 9 SLLPAAEIW S I/I/P\A\L\W\E-A 77.8 7 4 2 0 1 b-ion bias 98.243797 1.465502 999.5453 999.551 -0.006 -6.0 -1.4000000000000004 4.0 9207.3 nuORFdb_human ENST00000430998.6_1_10:4699836-4701809:- ENST00000430998.6_1_10:4699836-4701809:- LINC00704 lincRNA lncRNA GN=LINC00704 lincRNA E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep3_Slot2-5_1_3521 40mio-rep4 E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep4_Slot2-5_1_3522.7329.1445.2 2 1 1 8.13 5.41 3.01 2.72 63.6 5 1 1 15600.0 Hydrogen 79 (A) 8 SLCRLHTL (I) 8 SLDKVSHTL S/L/C R L|H\T\L 62.5 5 3 3 1 0 unbiased Carbamidomethylation C:Carbamidomethylation, 25.315912 0.848145 500.274 999.54 0.0003 0.3 5.0 9.49 30434.4 nuORFdb_human T198577_2:128172449-128174239:- T198577_2:128172449-128174239:- TUCP Other GN=nan TUCP E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep4_Slot2-5_1_3522 10mio-rep2 E_20221201_NO30_400nL_HLAc1_1e7_directIP_titration_rep2_Slot2-3_1_3513.7398.1462.2 2 1 1 11.38 5.3 5.33 6.08 70.3 7 1 1 4700.0 Hydrogen 100 (H) 9 SLDKVSHTL (-) 9 SLCRLHTL S L/D/K/V|S/H\T\L 77.8 7 3 5 1 0 unbiased 25.562838 0.837199 500.274 999.547 -0.0062 -6.2 0.5 6.46 12282.9 nuORFdb_human ENST00000513313.5_1_4:189321940-189407012:+ ENST00000513313.5_1_4:189321940-189407012:+ LINC01060 lincRNA lncRNA GN=LINC01060 lincRNA E_20221201_NO30_400nL_HLAc1_1e7_directIP_titration_rep2_Slot2-3_1_3513 10mio-rep3 E_20221201_NO30_400nL_HLAc1_1e7_directIP_titration_rep3_Slot2-3_1_3514.7409.1470.2 2 1 1 8.67 6.17 4.64 2.5 60.7 7 1 1 4130.0 Hydrogen 100 (H) 9 SLDKVSHTL (-) 9 SLCRLHTL S L/D\K/V\S|H\T\L 77.8 7 5 3 1 0 unbiased 25.601793 0.832431 500.275 999.547 -0.0042 -4.2 2.0999999999999996 6.46 12282.9 nuORFdb_human ENST00000513313.5_1_4:189321940-189407012:+ ENST00000513313.5_1_4:189321940-189407012:+ LINC01060 lincRNA lncRNA GN=LINC01060 lincRNA E_20221201_NO30_400nL_HLAc1_1e7_directIP_titration_rep3_Slot2-3_1_3514 20mio-rep1 E_20221201_NO30_400nL_HLAc1_2e7_directIP_titration_rep1_Slot2-4_1_3515.7311.1459.2 2 3 1 14.43 9.66 5.92 4.77 78.2 8 1 1 16200.0 Hydrogen 100 (H) 9 SLDKVSHTL (-) 9 SLCRLHTL S/L/D|K/V|S/H|T\L 100.0 9 4 7 3 0 dual b/y bias 25.258093 0.83904 500.2737 999.547 -0.0062 -6.2 0.0 6.46 12282.9 nuORFdb_human ENST00000513313.5_1_4:189321940-189407012:+ ENST00000513313.5_1_4:189321940-189407012:+ LINC01060 lincRNA lncRNA GN=LINC01060 lincRNA E_20221201_NO30_400nL_HLAc1_2e7_directIP_titration_rep1_Slot2-4_1_3515 20mio-rep2 E_20221201_NO30_400nL_HLAc1_2e7_directIP_titration_rep3_Slot2-4_1_3518.7481.1456.2 2 1 1 12.6 8.33 5.49 4.27 77.5 8 1 1 5010.0 Hydrogen 100 (H) 9 SLDKVSHTL (-) 9 SLCRLHTL S/L/D/K/V|S\H\T\L 100.0 9 4 5 1 0 unbiased 25.845865 0.841279 500.2725 999.547 -0.0082 -8.2 -1.8999999999999995 6.46 12282.9 nuORFdb_human ENST00000513313.5_1_4:189321940-189407012:+ ENST00000513313.5_1_4:189321940-189407012:+ LINC01060 lincRNA lncRNA GN=LINC01060 lincRNA E_20221201_NO30_400nL_HLAc1_2e7_directIP_titration_rep3_Slot2-4_1_3518 20mio-rep3 E_20221201_NO30_400nL_HLAc1_2e7_directIP_titration_rep4_Slot2-4_1_3519.7362.1454.2 2 3 1 15.1 6.91 6.42 8.19 81.9 8 1 1 19200.0 Hydrogen 100 (H) 9 SLDKVSHTL (-) 9 SLCRLHTL S/L/D|K|V|S\H\T\L 100.0 9 6 5 3 0 dual b/y bias 25.432415 0.842628 500.2738 999.547 -0.0062 -6.2 -0.40000000000000036 6.46 12282.9 nuORFdb_human ENST00000513313.5_1_4:189321940-189407012:+ ENST00000513313.5_1_4:189321940-189407012:+ LINC01060 lincRNA lncRNA GN=LINC01060 lincRNA E_20221201_NO30_400nL_HLAc1_2e7_directIP_titration_rep4_Slot2-4_1_3519 40mio-rep1 E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep1_Slot1-04_1_3496.7637.1433.2 2 3 1 17.31 12.04 6.53 5.27 88.0 8 1 1 82200.0 Hydrogen 100 (H) 9 SLDKVSHTL (-) 9 SLCRLHTL S/L/D|K/V\S|H|T\L 100.0 9 5 6 3 0 dual b/y bias 26.387569 0.85542 500.275 999.547 -0.0042 -4.2 -1.0 6.46 12282.9 nuORFdb_human ENST00000513313.5_1_4:189321940-189407012:+ ENST00000513313.5_1_4:189321940-189407012:+ LINC01060 lincRNA lncRNA GN=LINC01060 lincRNA E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep1_Slot1-04_1_3496 40mio-rep2 E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep2_Slot2-5_1_3520.7318.1446.2 2 6 1 14.15 6.63 6.7 7.52 74.3 8 1 1 75700.0 Hydrogen 100 (H) 9 SLDKVSHTL (-) 9 SLCRLHTL S/L/D/K/V\S|H|T\L 100.0 9 4 6 2 0 unbiased 25.285437 0.847201 500.2737 999.547 -0.0062 -6.2 -1.2999999999999998 6.46 12282.9 nuORFdb_human ENST00000513313.5_1_4:189321940-189407012:+ ENST00000513313.5_1_4:189321940-189407012:+ LINC01060 lincRNA lncRNA GN=LINC01060 lincRNA E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep2_Slot2-5_1_3520 40mio-rep3 E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep3_Slot2-5_1_3521.7324.1444.2 2 4 1 13.22 7.73 3.66 5.49 77.5 7 1 1 24200.0 Hydrogen 100 (H) 9 SLDKVSHTL (-) 9 LSHISTAGTL S/L/D|K/V/S H|T\L 77.8 7 3 6 2 0 y-ion bias 25.302948 0.848885 500.2743 999.547 -0.0062 -6.2 -1.6000000000000005 6.46 12282.9 nuORFdb_human ENST00000513313.5_1_4:189321940-189407012:+ ENST00000513313.5_1_4:189321940-189407012:+ LINC01060 lincRNA lncRNA GN=LINC01060 lincRNA E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep3_Slot2-5_1_3521 40mio-rep4 E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep4_Slot2-5_1_3522.7335.1684.3 3 1 1 10.12 8.43 2.93 1.69 84.9 7 1 1 6360.0 Hydrogen 100 (H) 9 SLDKVSHTL (-) 9 SLCRLHTL S/L/D/K/V S/H\T-L 77.8 7 1 5 0 1 y-ion bias 25.336611 0.696726 333.8521 999.547 -0.0055 -5.5 -0.7999999999999998 6.46 12282.9 nuORFdb_human ENST00000513313.5_1_4:189321940-189407012:+ ENST00000513313.5_1_4:189321940-189407012:+ LINC01060 lincRNA lncRNA GN=LINC01060 lincRNA E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep4_Slot2-5_1_3522 40mio-rep3 E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep3_Slot2-5_1_3521.24304.372.1 1 1 1 7.09 4.91 1.79 2.19 72.8 6 0 0 8510.0 Hydrogen 33 (P) 9 SLFTVLESI (Q) 9 VTSFIITnL S L F\T\V\L\E\S-I 66.7 6 5 0 0 1 b-ion bias 83.956139 1.501756 1008.5571 1008.561 -0.0012 -1.2 3.3999999999999995 4.0 7011.0 nuORFdb_human ENST00000438412.5_1_6:30259721-30259922:- ENST00000438412.5_1_6:30259721-30259922:- HCG18 Antisense lncRNA GN=HCG18 Antisense E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep3_Slot2-5_1_3521 40mio-rep1 E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep1_Slot1-04_1_3496.25059.1362.2 2 1 1 13.51 7.84 4.38 5.67 84.9 8 0 0 5900.0 Hydrogen 62 (L) 9 SLISTYFHV (-) 9 SLLSVVVcK S/L/I|S|T/Y/F/H-V 100.0 9 2 7 2 1 y-ion bias 86.569077 0.89984 533.7793 1066.557 -0.006 -5.7 -2.5 6.46 7876.0 smORF_v4|nuORFdb_human CCDC88A_2:55646277-55646489:- CCDC88A_2:55646277-55646489:-|ENST00000336838.10_1_2:55646279-55646603:- CCDC88A uORF 2:55646277-55646489:- 2 CCDC88A 5' uORF 5' uORF GN=CCDC88A E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep1_Slot1-04_1_3496 40mio-rep2 E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep2_Slot2-5_1_3520.22590.1488.2 2 1 1 7.3 4.68 2.65 2.62 54.6 6 0 1 6070.0 Hydrogen 3 (K) 10 SLLEGDQGGK (R) 10 SLIEnGQLE S/L/L|E\G D\Q|G G K 50.0 5 4 4 2 0 unbiased 78.038857 0.821005 502.2556 1003.505 -0.0007 -0.7 4.2 4.37 2974.4 nuORFdb_human ENST00000534499.1_1_11:82783686-82783770:+ ENST00000534499.1_1_11:82783686-82783770:+|ENST00000528156.1_1_11:82783686-82784364:+ RAB30-AS1 lincRNA lncRNA GN=RAB30-AS1 lincRNA E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep2_Slot2-5_1_3520 SCP E_20221201_NO30_400nL_HLAc1_4e7_PDAC_Slot2-6_1_3524.29623.354.1 1 2 1 11.8 10.03 2.12 1.77 81.4 6 0 0 17100.0 Hydrogen 48 (V) 9 SLLELDLGL (-) 9 SLEInILGL S L L\E|L\D|L\G\L 66.7 6 6 2 2 0 b-ion bias 102.346123 1.512393 972.5593 972.561 -0.0022 -2.3 0.10000000000000009 3.66 6238.1 nuORFdb_human ENST00000255559.7_1_17:70732822-70845907:- ENST00000255559.7_1_17:70732822-70845907:- SLC39A11 Out-of-Frame Out-of-Frame GN=SLC39A11 Out-of-Frame E_20221201_NO30_400nL_HLAc1_4e7_PDAC_Slot2-6_1_3524 40mio-rep1 E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep1_Slot1-04_1_3496.29351.340.1 1 1 1 12.32 9.86 2.25 2.46 73.5 7 0 0 16000.0 Hydrogen 48 (V) 9 SLLELDLGL (-) 9 SILLEDLAV S L/L|E|L\D|L\G\L 77.8 7 6 4 3 0 dual b/y bias 101.397552 1.520626 972.5593 972.561 -0.0022 -2.3 0.9000000000000004 3.66 6238.1 nuORFdb_human ENST00000255559.7_1_17:70732822-70845907:- ENST00000255559.7_1_17:70732822-70845907:- SLC39A11 Out-of-Frame Out-of-Frame GN=SLC39A11 Out-of-Frame E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep1_Slot1-04_1_3496 Eva-50mio-3fr AL20210827_EV_HLA_PDAC_PANFRO413_2D_50e6_W632_classI_fxn3.52515.52515.2 2 1 1 12.66 9.49 1.58 3.18 91.4 7 1 1 6260000.0 Hydrogen 3 (A) 9 SLLPLRVLL (L) 9 LTVLPRLVL S L|L|P/L/R\V\L/L 77.8 7 4 5 2 0 unbiased 0.979 98.2 94.53 100.0 0.0 512.3511 1023.692 0.0024 2.3 1.7999999999999998 9.47 2636.2 nuORFdb_human ENST00000397901.7_1_16:89720316-89724043:- ENST00000397901.7_1_16:89720316-89724043:- CHMP1A 5' Overlap uORF 5' Overlap uORF GN=CHMP1A 5' Overlap uORF AL20210827_EV_HLA_PDAC_PANFRO413_2D_50e6_W632_classI_fxn3 Exploris AL20221201_MKP_PDAC_01.67281.67281.2 2 1 1 8.57 7.02 0.56 1.55 79.7 6 1 1 46900000.0 Hydrogen 3 (A) 9 SLLPLRVLL (L) 9 SILPVILRL S L|L/P/L/R-V L/L 55.6 5 1 5 1 1 y-ion bias 0.998 78.2 100.55 15.64 0.0 512.3507 1023.692 0.0016 1.6 0.8 9.47 2636.2 nuORFdb_human ENST00000397901.7_1_16:89720316-89724043:- ENST00000397901.7_1_16:89720316-89724043:- CHMP1A 5' Overlap uORF 5' Overlap uORF GN=CHMP1A 5' Overlap uORF AL20221201_MKP_PDAC_01 40mio-rep2 E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep2_Slot2-5_1_3520.28246.463.1 1 1 1 8.43 2.87 6.29 5.56 73.8 6 0 0 5060.0 Hydrogen 10 (S) 9 SLLSGLWSA (W) 9 WIGSSILAS S L/L/S G|L\W\S-A 55.6 5 3 3 1 1 unbiased 97.577843 1.447978 933.4954 933.504 -0.009 -9.7 -4.799999999999999 5.24 11971.8 nuORFdb_human ENST00000612155.1_1_7:75912396-75915157:+ ENST00000612155.1_1_7:75912396-75915157:+ SRRM3 ncRNA Retained Intron lncRNA GN=SRRM3 ncRNA Retained Intron E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep2_Slot2-5_1_3520 40mio-rep4 E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep4_Slot2-5_1_3522.14219.1515.2 2 1 1 12.51 9.51 3.08 3.0 86.1 6 1 1 5850.0 Hydrogen 26 (L) 9 SLRVPSPAL (V) 9 LPSRVSPLA S L R|V-P|S|P\A\L 66.7 6 5 3 3 1 dual b/y bias 49.107624 0.803795 470.2805 939.562 -0.0075 -7.9 -3.2 9.47 5333.0 nuORFdb_human ENST00000474290.1_1_15:44069338-44069488:+ ENST00000474290.1_1_15:44069338-44069488:+ SERF2 5' uORF 5' uORF GN=SERF2 5' uORF E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep4_Slot2-5_1_3522 10mio-rep1 E_20221201_NO30_400nL_HLAc1_1e7_directIP_titration_rep1_Slot2-3_1_3512.14585.1305.2 2 1 1 14.66 3.37 10.93 11.29 69.2 7 1 1 2510.0 M6m Hydrogen 2 (M) 11 SLSSmSLQRTW (-) 11 SLSSFGRnGLSW S L/S|S-m|S L|Q|R\T\W 63.6 7 6 5 4 1 dual b/y bias m:Oxidized methionine 50.384335 0.935662 656.3158 1295.641 15.9835 -8.7 -0.9999999999999991 9.47 1426.7 nuORFdb_human ENST00000288607.2_1_7:1607452-1608764:- ENST00000288607.2_1_7:1607452-1608764:- PSMG3 Out-of-Frame Out-of-Frame GN=PSMG3 Out-of-Frame E_20221201_NO30_400nL_HLAc1_1e7_directIP_titration_rep1_Slot2-3_1_3512 10mio-rep3 E_20221201_NO30_400nL_HLAc1_1e7_directIP_titration_rep3_Slot2-3_1_3514.14510.1304.2 2 2 1 9.66 4.6 8.81 5.05 65.6 7 1 1 4340.0 M6m Hydrogen 2 (M) 11 SLSSmSLQRTW (-) 11 SLSSFGRnGLSW S L/S S/m/S L/Q|R/T\W 45.5 5 2 6 1 0 y-ion bias m:Oxidized methionine 50.127949 0.936085 656.3179 1295.641 15.9875 -5.7 0.5999999999999996 9.47 1426.7 nuORFdb_human ENST00000288607.2_1_7:1607452-1608764:- ENST00000288607.2_1_7:1607452-1608764:- PSMG3 Out-of-Frame Out-of-Frame GN=PSMG3 Out-of-Frame E_20221201_NO30_400nL_HLAc1_1e7_directIP_titration_rep3_Slot2-3_1_3514 20mio-rep1 E_20221201_NO30_400nL_HLAc1_2e7_directIP_titration_rep1_Slot2-4_1_3515.14498.1302.2 2 2 1 11.73 3.62 8.89 8.11 67.5 8 1 1 8830.0 M6m Hydrogen 2 (M) 11 SLSSmSLQRTW (-) 11 ISSMRKSQPYn S L/S/S m/S/L/Q/R\T\W 63.6 7 2 6 0 0 y-ion bias m:Oxidized methionine 50.073223 0.937344 656.3186 1295.641 15.9895 -4.1 2.1000000000000005 9.47 1426.7 nuORFdb_human ENST00000288607.2_1_7:1607452-1608764:- ENST00000288607.2_1_7:1607452-1608764:- PSMG3 Out-of-Frame Out-of-Frame GN=PSMG3 Out-of-Frame E_20221201_NO30_400nL_HLAc1_2e7_directIP_titration_rep1_Slot2-4_1_3515 20mio-rep2 E_20221201_NO30_400nL_HLAc1_2e7_directIP_titration_rep3_Slot2-4_1_3518.14585.1289.2 2 1 1 13.72 0.19 14.83 13.53 78.5 8 1 1 4340.0 M6m Hydrogen 2 (M) 11 SLSSmSLQRTW (-) 11 ISImAGDRAFSQ S L/S/S m/S/L|Q|R/T\W 63.6 7 3 7 2 0 y-ion bias m:Oxidized methionine 50.378212 0.945664 656.3167 1295.641 15.9855 -7.2 -0.9000000000000004 9.47 1426.7 nuORFdb_human ENST00000288607.2_1_7:1607452-1608764:- ENST00000288607.2_1_7:1607452-1608764:- PSMG3 Out-of-Frame Out-of-Frame GN=PSMG3 Out-of-Frame E_20221201_NO30_400nL_HLAc1_2e7_directIP_titration_rep3_Slot2-4_1_3518 20mio-rep3 E_20221201_NO30_400nL_HLAc1_2e7_directIP_titration_rep4_Slot2-4_1_3519.14518.1296.2 2 2 1 15.31 2.42 12.82 12.89 81.8 9 1 1 6340.0 M6m Hydrogen 2 (M) 11 SLSSmSLQRTW (-) 11 ISSMRKSQPYn S L/S/S/m|S|L/Q|R|T\W 81.8 9 5 8 4 0 dual b/y bias m:Oxidized methionine 50.146358 0.941068 656.319 1295.641 15.9895 -4.1 1.7000000000000002 9.47 1426.7 nuORFdb_human ENST00000288607.2_1_7:1607452-1608764:- ENST00000288607.2_1_7:1607452-1608764:- PSMG3 Out-of-Frame Out-of-Frame GN=PSMG3 Out-of-Frame E_20221201_NO30_400nL_HLAc1_2e7_directIP_titration_rep4_Slot2-4_1_3519 40mio-rep1 E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep1_Slot1-04_1_3496.14716.1257.2 2 3 1 17.52 6.48 12.5 11.04 81.5 9 1 1 26200.0 M6m Hydrogen 2 (M) 11 SLSSmSLQRTW (-) 11 SLSSFGRnGLSW S L/S|S/m/S/L|Q|R|T\W 81.8 9 5 8 4 0 dual b/y bias m:Oxidized methionine 50.833309 0.965388 656.3178 1295.641 15.9875 -5.7 -2.5 9.47 1426.7 nuORFdb_human ENST00000288607.2_1_7:1607452-1608764:- ENST00000288607.2_1_7:1607452-1608764:- PSMG3 Out-of-Frame Out-of-Frame GN=PSMG3 Out-of-Frame E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep1_Slot1-04_1_3496 40mio-rep2 E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep2_Slot2-5_1_3520.14480.1288.2 2 3 1 17.78 8.26 13.1 9.52 85.5 9 1 1 17100.0 M6m Hydrogen 2 (M) 11 SLSSmSLQRTW (-) 11 SLSSFGRnGLSW S L/S/S|m|S/L/Q|R|T\W 81.8 9 5 8 4 0 dual b/y bias m:Oxidized methionine 50.019508 0.946218 656.3179 1295.641 15.9875 -5.7 -0.7999999999999998 9.47 1426.7 nuORFdb_human ENST00000288607.2_1_7:1607452-1608764:- ENST00000288607.2_1_7:1607452-1608764:- PSMG3 Out-of-Frame Out-of-Frame GN=PSMG3 Out-of-Frame E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep2_Slot2-5_1_3520 40mio-rep3 E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep3_Slot2-5_1_3521.14502.1294.2 2 4 1 14.97 0.34 13.77 14.63 77.6 9 1 1 28700.0 M6m Hydrogen 2 (M) 11 SLSSmSLQRTW (-) 11 LSQARFSAcLP S L/S/S/m|S/L|Q|R|T\W 81.8 9 5 8 4 0 dual b/y bias m:Oxidized methionine 50.098522 0.942356 656.319 1295.641 15.9895 -4.1 0.5 9.47 1426.7 nuORFdb_human ENST00000288607.2_1_7:1607452-1608764:- ENST00000288607.2_1_7:1607452-1608764:- PSMG3 Out-of-Frame Out-of-Frame GN=PSMG3 Out-of-Frame E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep3_Slot2-5_1_3521 40mio-rep4 E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep4_Slot2-5_1_3522.14432.1276.2 2 3 1 19.57 6.77 13.27 12.8 82.1 9 1 1 20700.0 M6m Hydrogen 2 (M) 11 SLSSmSLQRTW (-) 11 SLSSFGRnGLSW S L/S|S/m|S/L|Q|R|T\W 81.8 9 6 8 5 0 dual b/y bias m:Oxidized methionine 49.842907 0.953645 656.3175 1295.641 15.9875 -5.7 -1.0 9.47 1426.7 nuORFdb_human ENST00000288607.2_1_7:1607452-1608764:- ENST00000288607.2_1_7:1607452-1608764:- PSMG3 Out-of-Frame Out-of-Frame GN=PSMG3 Out-of-Frame E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep4_Slot2-5_1_3522 10mio-rep1 E_20221201_NO30_400nL_HLAc1_1e7_directIP_titration_rep1_Slot2-3_1_3512.24977.572.1 1 1 1 12.25 7.99 3.74 4.26 77.0 7 0 0 3350.0 Hydrogen 88 (D) 9 SLTSLSLSV (C) 9 TVTSVLTSV S L/T|S|L|S\L\S\V 77.8 7 6 4 3 0 dual b/y bias 86.273903 1.38306 906.5078 906.514 -0.0062 -6.9 0.7999999999999998 5.24 11618.8 nuORFdb_human ENST00000467638.1_1_7:139757417-139762332:- ENST00000467638.1_1_7:139757417-139762332:- PARP12 ncRNA Retained Intron lncRNA GN=PARP12 ncRNA Retained Intron E_20221201_NO30_400nL_HLAc1_1e7_directIP_titration_rep1_Slot2-3_1_3512 10mio-rep2 E_20221201_NO30_400nL_HLAc1_1e7_directIP_titration_rep2_Slot2-3_1_3513.24913.570.1 1 1 1 10.04 5.6 3.21 4.44 74.1 6 0 0 2750.0 Hydrogen 88 (D) 9 SLTSLSLSV (C) 9 LSLTLSSSV S L/T\S|L|S\L\S-V 77.8 7 5 3 2 1 unbiased 86.045639 1.384171 906.5045 906.514 -0.0102 -11.3 -4.6000000000000005 5.24 11618.8 nuORFdb_human ENST00000467638.1_1_7:139757417-139762332:- ENST00000467638.1_1_7:139757417-139762332:- PARP12 ncRNA Retained Intron lncRNA GN=PARP12 ncRNA Retained Intron E_20221201_NO30_400nL_HLAc1_1e7_directIP_titration_rep2_Slot2-3_1_3513 10mio-rep3 E_20221201_NO30_400nL_HLAc1_1e7_directIP_titration_rep3_Slot2-3_1_3514.24880.565.1 1 1 1 10.36 6.74 2.62 3.61 72.2 7 0 0 3040.0 Hydrogen 88 (D) 9 SLTSLSLSV (C) 9 SLVTTRMV S L/T\S\L|S\L\S\V 77.8 7 6 2 1 0 b-ion bias 85.938728 1.386975 906.5049 906.514 -0.0092 -10.2 -3.8999999999999995 5.24 11618.8 nuORFdb_human ENST00000467638.1_1_7:139757417-139762332:- ENST00000467638.1_1_7:139757417-139762332:- PARP12 ncRNA Retained Intron lncRNA GN=PARP12 ncRNA Retained Intron E_20221201_NO30_400nL_HLAc1_1e7_directIP_titration_rep3_Slot2-3_1_3514 20mio-rep1 E_20221201_NO30_400nL_HLAc1_2e7_directIP_titration_rep1_Slot2-4_1_3515.24883.569.1 1 2 1 10.8 7.85 3.64 2.96 76.1 6 0 0 15000.0 Hydrogen 88 (D) 9 SLTSLSLSV (C) 9 LSLTLSSSV S L/T|S\L\S-L\S\V 77.8 7 5 2 1 1 b-ion bias 85.926147 1.384637 906.5055 906.514 -0.0092 -10.2 -3.999999999999999 5.24 11618.8 nuORFdb_human ENST00000467638.1_1_7:139757417-139762332:- ENST00000467638.1_1_7:139757417-139762332:- PARP12 ncRNA Retained Intron lncRNA GN=PARP12 ncRNA Retained Intron E_20221201_NO30_400nL_HLAc1_2e7_directIP_titration_rep1_Slot2-4_1_3515 20mio-rep2 E_20221201_NO30_400nL_HLAc1_2e7_directIP_titration_rep3_Slot2-4_1_3518.24899.560.1 1 1 1 9.5 6.74 2.28 2.76 77.9 6 0 0 4440.0 Hydrogen 88 (D) 9 SLTSLSLSV (C) 9 LSLTLSSSV S L/T|S\L\S-L\S-V 77.8 7 4 2 1 2 b-ion bias 86.000771 1.390208 906.5098 906.514 -0.0042 -4.7 1.5999999999999996 5.24 11618.8 nuORFdb_human ENST00000467638.1_1_7:139757417-139762332:- ENST00000467638.1_1_7:139757417-139762332:- PARP12 ncRNA Retained Intron lncRNA GN=PARP12 ncRNA Retained Intron E_20221201_NO30_400nL_HLAc1_2e7_directIP_titration_rep3_Slot2-4_1_3518 20mio-rep3 E_20221201_NO30_400nL_HLAc1_2e7_directIP_titration_rep4_Slot2-4_1_3519.24938.557.1 1 2 1 12.22 7.83 4.24 4.38 78.0 7 0 0 17200.0 Hydrogen 88 (D) 9 SLTSLSLSV (C) 9 LSLTLSSSV S L/T\S|L|S\L\S\V 77.8 7 6 3 2 0 b-ion bias 86.126961 1.391928 906.5078 906.514 -0.0062 -6.9 -1.1000000000000005 5.24 11618.8 nuORFdb_human ENST00000467638.1_1_7:139757417-139762332:- ENST00000467638.1_1_7:139757417-139762332:- PARP12 ncRNA Retained Intron lncRNA GN=PARP12 ncRNA Retained Intron E_20221201_NO30_400nL_HLAc1_2e7_directIP_titration_rep4_Slot2-4_1_3519 40mio-rep1 E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep1_Slot1-04_1_3496.25148.539.1 1 3 1 12.48 7.16 4.67 5.32 74.0 7 0 0 124000.0 Hydrogen 88 (D) 9 SLTSLSLSV (C) 9 TVTSVLTSV S L/T|S|L|S\L\S\V 77.8 7 6 4 3 0 dual b/y bias 86.876938 1.402886 906.5121 906.514 -0.0022 -2.5 0.7000000000000002 5.24 11618.8 nuORFdb_human ENST00000467638.1_1_7:139757417-139762332:- ENST00000467638.1_1_7:139757417-139762332:- PARP12 ncRNA Retained Intron lncRNA GN=PARP12 ncRNA Retained Intron E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep1_Slot1-04_1_3496 40mio-rep2 E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep2_Slot2-5_1_3520.24928.554.1 1 2 1 11.4 6.53 3.73 4.87 77.5 5 0 0 82700.0 Hydrogen 88 (D) 9 SLTSLSLSV (C) 9 STTVLIATT S L/T|S|L|S-L\S-V 77.8 7 4 4 3 2 dual b/y bias 86.113213 1.393578 906.51 906.514 -0.0042 -4.7 0.20000000000000018 5.24 11618.8 nuORFdb_human ENST00000467638.1_1_7:139757417-139762332:- ENST00000467638.1_1_7:139757417-139762332:- PARP12 ncRNA Retained Intron lncRNA GN=PARP12 ncRNA Retained Intron E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep2_Slot2-5_1_3520 40mio-rep3 E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep3_Slot2-5_1_3521.24928.554.1 1 4 1 10.02 7.0 2.26 3.02 74.1 6 0 0 66900.0 Hydrogen 88 (D) 9 SLTSLSLSV (C) 9 LSLTLSSSV S L/T\S\L|S-L\S\V 77.8 7 5 2 1 1 b-ion bias 86.111435 1.393511 906.5096 906.514 -0.0042 -4.7 -0.10000000000000053 5.24 11618.8 nuORFdb_human ENST00000467638.1_1_7:139757417-139762332:- ENST00000467638.1_1_7:139757417-139762332:- PARP12 ncRNA Retained Intron lncRNA GN=PARP12 ncRNA Retained Intron E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep3_Slot2-5_1_3521 40mio-rep4 E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep4_Slot2-5_1_3522.24950.553.1 1 2 1 12.16 7.03 4.41 5.13 77.2 6 0 0 73000.0 Hydrogen 88 (D) 9 SLTSLSLSV (C) 9 LSLTLSSSV S L/T|S|L|S-L\S\V 77.8 7 5 4 3 1 dual b/y bias 86.175423 1.394461 906.511 906.514 -0.0032 -3.6 1.1 5.24 11618.8 nuORFdb_human ENST00000467638.1_1_7:139757417-139762332:- ENST00000467638.1_1_7:139757417-139762332:- PARP12 ncRNA Retained Intron lncRNA GN=PARP12 ncRNA Retained Intron E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep4_Slot2-5_1_3522 10mio-rep1 E_20221201_NO30_400nL_HLAc1_1e7_directIP_titration_rep1_Slot2-3_1_3512.20018.533.1 1 1 1 8.69 7.14 2.14 1.55 61.3 6 0 0 5720.0 Hydrogen 7 (D) 9 SLYDVPVGA (A) 9 YnSLVPVQ S L/Y\D|V|P\V\G A 55.6 5 5 3 2 0 unbiased 69.149994 1.406113 920.466 920.472 -0.0064 -6.9 0.7999999999999998 3.8 2654.9 nuORFdb_human ENST00000379312.7_3_16:67562815-67572402:+ ENST00000379312.7_3_16:67562815-67572402:+|ENST00000540839.7_3_16:67563547-67572402:+|ENST00000566920.5_3_16:67563967-67572402:+|ENST00000565176.5_3_16:67565474-67572402:+|ENST00000566559.5_3_16:67572255-67572402:+ FAM65A 5' Overlap uORF 5' Overlap uORF GN=FAM65A 5' Overlap uORF E_20221201_NO30_400nL_HLAc1_1e7_directIP_titration_rep1_Slot2-3_1_3512 20mio-rep3 E_20221201_NO30_400nL_HLAc1_2e7_directIP_titration_rep4_Slot2-4_1_3519.19934.521.1 1 1 1 8.12 6.01 2.24 2.12 66.9 6 0 0 25800.0 Hydrogen 7 (D) 9 SLYDVPVGA (A) 9 YnSLVPVQ S L/Y\D|V|P\V-G A 55.6 5 4 3 2 1 unbiased 68.847672 1.413624 920.4681 920.472 -0.0044 -4.8 1.0 3.8 2654.9 nuORFdb_human ENST00000379312.7_3_16:67562815-67572402:+ ENST00000379312.7_3_16:67562815-67572402:+|ENST00000540839.7_3_16:67563547-67572402:+|ENST00000566920.5_3_16:67563967-67572402:+|ENST00000565176.5_3_16:67565474-67572402:+|ENST00000566559.5_3_16:67572255-67572402:+ FAM65A 5' Overlap uORF 5' Overlap uORF GN=FAM65A 5' Overlap uORF E_20221201_NO30_400nL_HLAc1_2e7_directIP_titration_rep4_Slot2-4_1_3519 40mio-rep1 E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep1_Slot1-04_1_3496.20121.498.1 1 2 1 14.43 10.95 5.1 3.48 83.9 7 0 0 75100.0 Hydrogen 7 (D) 9 SLYDVPVGA (A) 9 LSVnYPVGA S L/Y|D|V|P|V\G\A 77.8 7 6 5 4 0 dual b/y bias 69.508392 1.426873 920.4697 920.472 -0.0024 -2.6 0.6000000000000001 3.8 2654.9 nuORFdb_human ENST00000379312.7_3_16:67562815-67572402:+ ENST00000379312.7_3_16:67562815-67572402:+|ENST00000540839.7_3_16:67563547-67572402:+|ENST00000566920.5_3_16:67563967-67572402:+|ENST00000565176.5_3_16:67565474-67572402:+|ENST00000566559.5_3_16:67572255-67572402:+ FAM65A 5' Overlap uORF 5' Overlap uORF GN=FAM65A 5' Overlap uORF E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep1_Slot1-04_1_3496 40mio-rep4 E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep4_Slot2-5_1_3522.19804.515.1 1 1 1 11.53 9.0 3.26 2.54 83.1 7 0 0 12400.0 Hydrogen 7 (D) 9 SLYDVPVGA (A) 9 LSVnYPVGA S L/Y/D|V|P\V\G\A 77.8 7 5 4 2 0 unbiased 68.39637 1.417196 920.4689 920.472 -0.0034 -3.7 1.0 3.8 2654.9 nuORFdb_human ENST00000379312.7_3_16:67562815-67572402:+ ENST00000379312.7_3_16:67562815-67572402:+|ENST00000540839.7_3_16:67563547-67572402:+|ENST00000566920.5_3_16:67563967-67572402:+|ENST00000565176.5_3_16:67565474-67572402:+|ENST00000566559.5_3_16:67572255-67572402:+ FAM65A 5' Overlap uORF 5' Overlap uORF GN=FAM65A 5' Overlap uORF E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep4_Slot2-5_1_3522 5mio-rep2 E_20221201_NO30_400nL_HLAc1_5e6_directIP_titration_rep2_Slot2-2_1_3510.20129.533.1 1 1 1 8.8 6.15 2.56 2.65 68.7 6 0 0 2590.0 Hydrogen 7 (D) 9 SLYDVPVGA (A) 9 YnSLVPVQ S L Y\D|V|P\V\G\A 66.7 6 6 2 2 0 b-ion bias 69.52227 1.406085 920.4623 920.472 -0.0104 -11.3 -2.6000000000000014 3.8 2654.9 nuORFdb_human ENST00000379312.7_3_16:67562815-67572402:+ ENST00000379312.7_3_16:67562815-67572402:+|ENST00000540839.7_3_16:67563547-67572402:+|ENST00000566920.5_3_16:67563967-67572402:+|ENST00000565176.5_3_16:67565474-67572402:+|ENST00000566559.5_3_16:67572255-67572402:+ FAM65A 5' Overlap uORF 5' Overlap uORF GN=FAM65A 5' Overlap uORF E_20221201_NO30_400nL_HLAc1_5e6_directIP_titration_rep2_Slot2-2_1_3510 10mio-rep1 E_20221201_NO30_400nL_HLAc1_1e7_directIP_titration_rep1_Slot2-3_1_3512.24069.409.1 1 3 1 13.72 8.76 8.58 4.97 90.6 7 0 0 5050.0 Hydrogen 47 (S) 9 SLYPELADV (Q) 9 ISYPLEnAV S L/Y|P/E\L\A\D\V 77.8 7 5 3 1 0 unbiased 83.13871 1.480203 1006.499 1006.509 -0.0092 -9.1 -1.3999999999999995 3.67 7541.2 nuORFdb_human ENST00000475325.1_1_10:13688935-13696522:- ENST00000475325.1_1_10:13688935-13696522:- FRMD4A 3' Overlap dORF 3' Overlap dORF GN=FRMD4A 3' Overlap dORF E_20221201_NO30_400nL_HLAc1_1e7_directIP_titration_rep1_Slot2-3_1_3512 10mio-rep2 E_20221201_NO30_400nL_HLAc1_1e7_directIP_titration_rep2_Slot2-3_1_3513.24014.401.1 1 1 1 11.03 5.93 7.05 5.1 74.1 6 0 0 1960.0 Hydrogen 47 (S) 9 SLYPELADV (Q) 9 ISYPLEnAV S L/Y/P E|L\A\D\V 55.6 5 4 3 1 0 unbiased 82.941643 1.484588 1006.5048 1006.509 -0.0092 -9.1 -2.3999999999999995 3.67 7541.2 nuORFdb_human ENST00000475325.1_1_10:13688935-13696522:- ENST00000475325.1_1_10:13688935-13696522:- FRMD4A 3' Overlap dORF 3' Overlap dORF GN=FRMD4A 3' Overlap dORF E_20221201_NO30_400nL_HLAc1_1e7_directIP_titration_rep2_Slot2-3_1_3513 20mio-rep1 E_20221201_NO30_400nL_HLAc1_2e7_directIP_titration_rep1_Slot2-4_1_3515.23965.408.1 1 4 1 13.52 9.08 8.13 4.45 85.4 7 0 0 7640.0 Hydrogen 47 (S) 9 SLYPELADV (Q) 9 ISYPLEnAV S L/Y|P\E\L\A\D\V 77.8 7 6 2 1 0 b-ion bias 82.757301 1.48072 1006.4985 1006.509 -0.0092 -9.1 -2.8999999999999995 3.67 7541.2 nuORFdb_human ENST00000475325.1_1_10:13688935-13696522:- ENST00000475325.1_1_10:13688935-13696522:- FRMD4A 3' Overlap dORF 3' Overlap dORF GN=FRMD4A 3' Overlap dORF E_20221201_NO30_400nL_HLAc1_2e7_directIP_titration_rep1_Slot2-4_1_3515 Eva-50mio-3fr AL20210827_EV_HLA_PDAC_PANFRO413_2D_50e6_W632_classI_fxn1.13402.13402.2 2 1 1 11.97 9.9 3.25 2.07 96.3 7 1 1 3530000.0 Hydrogen 16 (N) 10 SPAATRGPSL (A) 10 SPAFHTALL S/P|A|A/T/R G\P S/L 60.0 6 3 6 2 0 y-ion bias 0.984 94.8 26.12 100.0 0.0 478.7616 956.516 0.0 0.0 -0.1 9.47 61769.9 nuORFdb_human ENST00000370082.1_1_20:62201547-62204491:- ENST00000370082.1_1_20:62201547-62204491:-|ENST00000479540.5_1_20:62203456-62204512:- HELZ2 5' Overlap uORF 5' Overlap uORF GN=HELZ2 5' Overlap uORF AL20210827_EV_HLA_PDAC_PANFRO413_2D_50e6_W632_classI_fxn1 SCP E_20221201_NO30_400nL_HLAc1_4e7_PDAC_Slot2-6_1_3524.7144.1533.2 2 2 1 9.99 7.3 5.81 2.69 63.7 6 1 1 31500.0 Hydrogen 16 (N) 10 SPAATRGPSL (A) 10 APSAARSLPS S/P/A|A|T R|G|P S-L 60.0 6 4 6 4 1 dual b/y bias 24.68461 0.792567 478.759 956.516 -0.0052 -5.5 -3.1 9.47 61769.9 nuORFdb_human ENST00000370082.1_1_20:62201547-62204491:- ENST00000370082.1_1_20:62201547-62204491:-|ENST00000479540.5_1_20:62203456-62204512:- HELZ2 5' Overlap uORF 5' Overlap uORF GN=HELZ2 5' Overlap uORF E_20221201_NO30_400nL_HLAc1_4e7_PDAC_Slot2-6_1_3524 Exploris_FAIMS VH20221207_MKP_FAIMS2022E_HLAc1_4e7_PDAC.20854.20854.2 2 1 1 17.15 12.72 7.06 4.43 94.9 9 1 1 7380000.0 Hydrogen 16 (N) 10 SPAATRGPSL (A) 10 APSAARSLPS S/P|A|A/T/R/G|P\S/L 100.0 10 4 8 3 0 y-ion bias 0.996 97.8 43.83 120.0 -50.0 478.7605 956.516 -0.0023 -2.4 0.20000000000000018 9.47 61769.9 nuORFdb_human ENST00000370082.1_1_20:62201547-62204491:- ENST00000370082.1_1_20:62201547-62204491:-|ENST00000479540.5_1_20:62203456-62204512:- HELZ2 5' Overlap uORF 5' Overlap uORF GN=HELZ2 5' Overlap uORF VH20221207_MKP_FAIMS2022E_HLAc1_4e7_PDAC SCP E_20221201_NO30_400nL_HLAc1_4e7_PDAC_Slot2-6_1_3524.11852.683.1 1 2 1 10.48 5.59 6.18 4.89 84.0 6 0 0 19300.0 Hydrogen 4 (S) 9 SPAAWTSAA (R) 9 SPGDRTSAA S P A/A|W\T\S\A\A 66.7 6 5 2 1 0 b-ion bias 40.945236 1.316491 861.4069 861.41 -0.0031 -3.6 -1.2000000000000002 5.24 2438.8 nuORFdb_human ENST00000598273.5_1_19:48284145-48284407:+ ENST00000598273.5_1_19:48284145-48284407:+ SELENOW ncRNA Retained Intron lncRNA GN=SELENOW ncRNA Retained Intron E_20221201_NO30_400nL_HLAc1_4e7_PDAC_Slot2-6_1_3524 SCP E_20221201_NO30_400nL_HLAc1_4e7_PDAC_Slot2-6_1_3524.6832.777.1 1 1 1 9.13 5.38 2.89 3.75 68.2 8 0 0 3360.0 Hydrogen 37 (G) 9 SPAGQAGGL (-) 9 SPGAQAAVG S/P/A/G|Q\A\G\G\L 100.0 9 5 4 1 0 unbiased 23.60689 1.259841 757.3845 757.384 0.0001 0.1 2.5 5.24 4204.8 nuORFdb_human ENST00000356206.10_1_8:23307502-23315196:- ENST00000356206.10_1_8:23307502-23315196:- ENTPD4 5' uORF 5' uORF GN=ENTPD4 5' uORF E_20221201_NO30_400nL_HLAc1_4e7_PDAC_Slot2-6_1_3524 SCP E_20221201_NO30_400nL_HLAc1_4e7_PDAC_Slot2-6_1_3524.8416.1426.2 2 1 1 12.44 6.32 5.54 6.12 78.1 7 1 1 5370.0 Hydrogen 58 (S) 9 SPCRSVTQF (T) 9 qPFLMSTQF S/P/C/R\S-V|T|Q\F 100.0 9 4 5 2 1 unbiased Carbamidomethylation C:Carbamidomethylation, 29.075796 0.860059 541.2583 1081.509 -0.0008 -0.7 1.7 9.47 11928.6 nuORFdb_human ENST00000410074.5_2_8:25042331-25128363:+ ENST00000410074.5_2_8:25042331-25128363:+ DOCK5 5' Overlap uORF 5' Overlap uORF GN=DOCK5 5' Overlap uORF E_20221201_NO30_400nL_HLAc1_4e7_PDAC_Slot2-6_1_3524 SCP E_20221201_NO30_400nL_HLAc1_4e7_PDAC_Slot2-6_1_3524.13623.422.1 1 1 1 9.82 4.98 7.36 4.84 80.9 5 0 0 11500.0 Hydrogen 15 (I) 9 SPESGNYLY (L) 9 STPnNGYLY S P E|S-G-N\Y\L\Y 66.7 6 4 1 1 2 b-ion bias 47.063759 1.472176 1029.4496 1029.452 -0.0024 -2.3 0.10000000000000009 4.0 5818.8 nuORFdb_human ENST00000439706.5_1_12:46637015-46648661:- ENST00000439706.5_1_12:46637015-46648661:- SLC38A1 5' uORF 5' uORF GN=SLC38A1 5' uORF E_20221201_NO30_400nL_HLAc1_4e7_PDAC_Slot2-6_1_3524 SCP E_20221201_NO30_400nL_HLAc1_4e7_PDAC_Slot2-6_1_3524.14996.1435.2 2 1 1 11.05 6.3 5.99 4.75 60.2 7 1 1 39500.0 Hydrogen 79 (L) 9 SPLSKSWEM (V) 9 SLPSSWKEM S/P/L|S|K S/W\E\M 77.8 7 4 5 2 0 unbiased 51.807934 0.854623 532.7557 1064.508 -0.0034 -3.2 -0.8000000000000003 5.72 12588.4 smORF_v4|nuORFdb_human LINC00649_21:35303728-35318139:+ LINC00649_21:35303728-35318139:+|TCONS_00028768_21:35303769-35318136:+|ENST00000599421.1_1_21:35303901-35310602:+ LINC00649 lncRNA 21:35303728-35318139:+ 21 linc-SLC5A3-2 lincRNA lncRNA GN=LINC00649 E_20221201_NO30_400nL_HLAc1_4e7_PDAC_Slot2-6_1_3524 Eva-50mio-3fr AL20210827_EV_HLA_PDAC_PANFRO413_2D_50e6_W632_classI_fxn2.17266.17266.2 2 2 2 16.86 10.06 7.38 6.79 97.4 8 1 1 11100000.0 M87m Hydrogen 79 (L) 9 SPLSKSWEm (V) 9 SLPSSWKEm S/P|L|S/K/S/W/E/m 100.0 9 2 8 2 0 y-ion bias m:Oxidized methionine 0.984 97.7 33.13 100.0 0.0 540.7556 1064.508 15.9957 0.7 0.39999999999999997 5.72 12588.4 smORF_v4|nuORFdb_human LINC00649_21:35303728-35318139:+ LINC00649_21:35303728-35318139:+|TCONS_00028768_21:35303769-35318136:+|ENST00000599421.1_1_21:35303901-35310602:+ LINC00649 lncRNA 21:35303728-35318139:+ 21 linc-SLC5A3-2 lincRNA lncRNA GN=LINC00649 AL20210827_EV_HLA_PDAC_PANFRO413_2D_50e6_W632_classI_fxn2 Exploris AL20221201_MKP_PDAC_01.22023.22023.2 2 1 1 11.27 6.2 4.92 5.07 73.5 8 1 1 107000000.0 M87m Hydrogen 79 (L) 9 SPLSKSWEm (V) 9 SPImTGKEMA S/P|L|S/K/S-W/E/m 100.0 9 2 7 2 1 y-ion bias m:Oxidized methionine 0.86 86.5 37.3 100.0 0.0 540.7554 1064.508 15.9955 0.5 -0.30000000000000004 5.72 12588.4 smORF_v4|nuORFdb_human LINC00649_21:35303728-35318139:+ LINC00649_21:35303728-35318139:+|TCONS_00028768_21:35303769-35318136:+|ENST00000599421.1_1_21:35303901-35310602:+ LINC00649 lncRNA 21:35303728-35318139:+ 21 linc-SLC5A3-2 lincRNA lncRNA GN=LINC00649 AL20221201_MKP_PDAC_01 Exploris_FAIMS VH20221207_MKP_FAIMS2022E_HLAc1_4e7_PDAC.27802.27802.2 2 1 1 16.9 9.43 9.71 7.46 90.1 8 1 1 35400000.0 M87m Hydrogen 79 (L) 9 SPLSKSWEm (V) 9 SLPSSWKEm S/P|L|S|K/S/W/E/m 100.0 9 3 8 3 0 dual b/y bias m:Oxidized methionine 0.988 99.2 55.57 20.59 -50.0 540.754 1064.508 15.9927 -2.1 0.5 5.72 12588.4 smORF_v4|nuORFdb_human LINC00649_21:35303728-35318139:+ LINC00649_21:35303728-35318139:+|TCONS_00028768_21:35303769-35318136:+|ENST00000599421.1_1_21:35303901-35310602:+ LINC00649 lncRNA 21:35303728-35318139:+ 21 linc-SLC5A3-2 lincRNA lncRNA GN=LINC00649 VH20221207_MKP_FAIMS2022E_HLAc1_4e7_PDAC Exploris AL20221201_MKP_PDAC_01.65671.65671.2 2 1 1 16.28 13.08 16.28 89.6 7 0 0 108000000.0 Hydrogen 70 (S) 11 SPMAVLLHGLY (T) 11 S P|M|A|V/L L/H G-L/Y 45.5 5 3 6 3 1 y-ion bias 1.0 90.2 99.35 36.23 0.0 600.8262 1200.645 0.0006 0.5 -0.30000000000000004 6.46 10544.1 nuORFdb_human ENST00000579991.2_1_17:56166528-56166822:+ ENST00000579991.2_1_17:56166528-56166822:+ DYNLL2 3' dORF 3' dORF GN=DYNLL2 3' dORF AL20221201_MKP_PDAC_01 SCP E_20221201_NO30_400nL_HLAc1_4e7_PDAC_Slot2-6_1_3524.13210.518.1 1 2 1 16.08 6.85 9.27 9.23 91.9 8 0 0 12900.0 Hydrogen 56 (M) 10 SPPAAAAACL (-) 10 PTAPAGAACL S/P/P A|A\A\A|A\C\L 80.0 8 6 4 2 0 unbiased Carbamidomethylation C:Carbamidomethylation, 45.63789 1.415111 928.4491 928.456 -0.0067 -7.2 -4.800000000000001 5.24 6387.2 nuORFdb_human ENST00000303004.4_1_20:48807437-48807635:+ ENST00000303004.4_1_20:48807437-48807635:+ CEBPB 5' Overlap uORF 5' Overlap uORF GN=CEBPB 5' Overlap uORF E_20221201_NO30_400nL_HLAc1_4e7_PDAC_Slot2-6_1_3524 Exploris_FAIMS VH20221207_MKP_FAIMS2022E_HLAc1_4e7_PDAC.34596.34596.2 2 1 1 12.09 3.26 11.62 8.83 67.0 7 0 0 6430000.0 Hydrogen 56 (M) 10 SPPAAAAACL (-) 10 PTAPAGAACL S P\P A\A\A\A\A|C/L 60.0 6 6 2 1 0 b-ion bias Carbamidomethylation C:Carbamidomethylation, 1.0 68.8 65.45 19.6 -70.0 464.7301 928.456 -0.0027 -3.0 -0.3999999999999999 5.24 6387.2 nuORFdb_human ENST00000303004.4_1_20:48807437-48807635:+ ENST00000303004.4_1_20:48807437-48807635:+ CEBPB 5' Overlap uORF 5' Overlap uORF GN=CEBPB 5' Overlap uORF VH20221207_MKP_FAIMS2022E_HLAc1_4e7_PDAC Eva-50mio-3fr AL20210827_EV_HLA_PDAC_PANFRO413_2D_50e6_W632_classI_fxn1.19524.19524.2 2 1 1 8.49 3.74 5.94 4.75 62.2 6 0 0 5490000.0 C64c Hydrogen 56 (M) 10 SPPAAAAAcL (-) 10 SPHTTLcL S P|P A A-A-A\A|c/L 50.0 5 3 3 2 2 unbiased c:Cysteinylation 0.982 67.3 36.7 100.0 0.0 495.7234 928.456 61.9838 1.2 1.0999999999999999 5.24 6387.2 nuORFdb_human ENST00000303004.4_1_20:48807437-48807635:+ ENST00000303004.4_1_20:48807437-48807635:+ CEBPB 5' Overlap uORF 5' Overlap uORF GN=CEBPB 5' Overlap uORF AL20210827_EV_HLA_PDAC_PANFRO413_2D_50e6_W632_classI_fxn1 SCP E_20221201_NO30_400nL_HLAc1_4e7_PDAC_Slot2-6_1_3524.12459.1410.2 2 1 1 13.95 5.34 8.69 8.61 79.5 8 0 0 13300.0 Hydrogen 23 (D) 9 SPQVEVHEF (A) 9 SPGAnLVFHE S/P/Q|V|E\V|H\E\F 100.0 9 6 5 3 0 dual b/y bias 43.041515 0.870126 536.2567 1071.511 -0.0038 -3.6 -1.2000000000000002 4.51 3591.8 nuORFdb_human ENST00000336985.10_2_22:45098296-45098401:+ ENST00000336985.10_2_22:45098296-45098401:+ PRR5 5' Overlap uORF 5' Overlap uORF GN=PRR5 5' Overlap uORF E_20221201_NO30_400nL_HLAc1_4e7_PDAC_Slot2-6_1_3524 Exploris_FAIMS VH20221207_MKP_FAIMS2022E_HLAc1_4e7_PDAC.33015.33015.2 2 1 1 12.57 4.94 12.57 7.63 78.7 7 0 0 5480000.0 Hydrogen 23 (D) 9 SPQVEVHEF (A) 9 SPGAnLVFHE S/P|Q|V/E V/H-E-F 77.8 7 2 5 2 2 y-ion bias 0.989 87.3 63.17 64.53 -70.0 536.2573 1071.511 -0.0033 -3.0 -0.3999999999999999 4.51 3591.8 nuORFdb_human ENST00000336985.10_2_22:45098296-45098401:+ ENST00000336985.10_2_22:45098296-45098401:+ PRR5 5' Overlap uORF 5' Overlap uORF GN=PRR5 5' Overlap uORF VH20221207_MKP_FAIMS2022E_HLAc1_4e7_PDAC SCP E_20221201_NO30_400nL_HLAc1_4e7_PDAC_Slot2-6_1_3524.3614.1675.3 3 1 1 10.7 5.23 3.89 5.47 83.1 7 2 2 10200.0 Hydrogen 44 (G) 10 SPRAPGGNRL (A) 10 SPARGVAGPRG S/P/R/A/P/G|G/N R L 70.0 7 1 7 1 0 y-ion bias 12.498028 0.702841 342.1912 1024.565 -0.0062 -6.1 -3.6999999999999997 12.0 9600.9 nuORFdb_human ENST00000360325.11_1_17:7165522-7165789:- ENST00000360325.11_1_17:7165522-7165789:- CLDN7 5' uORF 5' uORF GN=CLDN7 5' uORF E_20221201_NO30_400nL_HLAc1_4e7_PDAC_Slot2-6_1_3524 Exploris_FAIMS VH20221207_MKP_FAIMS2022E_HLAc1_4e7_PDAC.21910.21910.3 3 1 1 10.59 2.11 10.59 8.47 76.5 6 2 2 2470000.0 Hydrogen 29 (A) 10 SPSMGRNLKM (R) 10 SPKRAGWSTM S/P|S/M/G R N L/K/M 60.0 6 1 6 1 0 y-ion bias 0.952 100.0 45.77 105.38 -70.0 374.1905 1120.56 -0.0033 -3.0 -0.3999999999999999 11.0 4633.5 nuORFdb_human ENST00000415933.5_1_7:44839032-44839382:+ ENST00000415933.5_1_7:44839032-44839382:+ PPIA Out-of-Frame Out-of-Frame GN=PPIA Out-of-Frame VH20221207_MKP_FAIMS2022E_HLAc1_4e7_PDAC Eva-50mio-3fr AL20210827_EV_HLA_PDAC_PANFRO413_2D_50e6_W632_classI_fxn1.11941.12034.2 2 1 1 19.3 16.62 5.54 2.67 86.8 8 0 1 25900000.0 Hydrogen 21 (G) 9 SPSRPPSSL (-) 9 SPRSPSPSL S/P|S/R|P\P\S|S|L 100.0 9 6 6 4 0 dual b/y bias 0.996 88.8 23.65 100.0 0.0 464.2485 927.489 0.0002 0.3 0.19999999999999998 9.47 2923.2 nuORFdb_human ENST00000621795.1_1_19:288015-288105:- ENST00000621795.1_1_19:288015-288105:-|ENST00000327790.7_1_19:287630-288075:- PLPP2 ncRNA Retained Intron lncRNA GN=PLPP2 ncRNA Retained Intron AL20210827_EV_HLA_PDAC_PANFRO413_2D_50e6_W632_classI_fxn1 Exploris AL20221201_MKP_PDAC_01.14411.14541.2 2 1 1 20.14 18.54 5.03 1.59 93.3 8 0 1 89200000.0 Hydrogen 21 (G) 9 SPSRPPSSL (-) 9 SPRSPSPSL S/P|S/R|P\P\S|S|L 100.0 9 6 6 4 0 dual b/y bias 0.99 100.0 26.73 100.0 0.0 464.2489 927.489 0.0012 1.3 0.5 9.47 2923.2 nuORFdb_human ENST00000621795.1_1_19:288015-288105:- ENST00000621795.1_1_19:288015-288105:-|ENST00000327790.7_1_19:287630-288075:- PLPP2 ncRNA Retained Intron lncRNA GN=PLPP2 ncRNA Retained Intron AL20221201_MKP_PDAC_01 SCP E_20221201_NO30_400nL_HLAc1_4e7_PDAC_Slot2-6_1_3524.6283.1508.2 2 1 1 16.36 15.07 3.82 1.28 87.7 8 0 1 40500.0 Hydrogen 21 (G) 9 SPSRPPSSL (-) 9 SPRSPSPSL S/P/S|R|P\P\S\S\L 100.0 9 6 4 2 0 unbiased 21.711422 0.80865 464.2465 927.489 -0.0027 -2.9 -0.5 9.47 2923.2 nuORFdb_human ENST00000621795.1_1_19:288015-288105:- ENST00000621795.1_1_19:288015-288105:-|ENST00000327790.7_1_19:287630-288075:- PLPP2 ncRNA Retained Intron lncRNA GN=PLPP2 ncRNA Retained Intron E_20221201_NO30_400nL_HLAc1_4e7_PDAC_Slot2-6_1_3524 Exploris_FAIMS VH20221207_MKP_FAIMS2022E_HLAc1_4e7_PDAC.18790.19010.2 2 1 1 20.01 17.84 5.59 2.17 90.5 8 0 1 64500000.0 Hydrogen 21 (G) 9 SPSRPPSSL (-) 9 SPRSPSPSL S/P|S/R|P\P\S|S|L 100.0 9 6 6 4 0 dual b/y bias 0.996 98.6 40.02 44.57 -50.0 464.2474 927.489 -0.002 -2.1 0.5 9.47 2923.2 nuORFdb_human ENST00000621795.1_1_19:288015-288105:- ENST00000621795.1_1_19:288015-288105:-|ENST00000327790.7_1_19:287630-288075:- PLPP2 ncRNA Retained Intron lncRNA GN=PLPP2 ncRNA Retained Intron VH20221207_MKP_FAIMS2022E_HLAc1_4e7_PDAC Exploris_FAIMS VH20221207_MKP_FAIMS2022E_HLAc1_4e7_PDAC.21856.21856.3 3 1 1 11.47 9.5 1.47 1.97 76.0 9 2 2 3060000.0 Hydrogen 69 (R) 11 SPSRTTSRLAL (R) 11 SPTVNTTLRSL S/P|S/R/T/T/S R-L/A/L 81.8 9 1 8 1 1 y-ion bias 0.987 100.0 45.68 120.0 -70.0 396.8935 1188.669 -0.0036 -3.0 -0.3999999999999999 12.0 13055.1 nuORFdb_human ENST00000526792.5_1_1:26857792-26873731:+ ENST00000526792.5_1_1:26857792-26873731:+|ENST00000374163.5_1_1:26872488-26873731:+ RPS6KA1 3' Overlap dORF 3' Overlap dORF GN=RPS6KA1 3' Overlap dORF VH20221207_MKP_FAIMS2022E_HLAc1_4e7_PDAC SCP E_20221201_NO30_400nL_HLAc1_4e7_PDAC_Slot2-6_1_3524.4932.1388.2 2 1 1 11.74 8.14 4.71 3.6 74.0 7 1 2 7090.0 Hydrogen 2 (M) 10 SPSSTRKVPL (-) 10 SPIInAFVLP S/P/S/S T/R K\V|P-L 60.0 6 2 5 1 1 y-ion bias 17.047213 0.883897 536.3094 1071.616 -0.005 -4.6 -2.1999999999999997 11.0 1202.4 nuORFdb_human ENST00000543953.5_1_X:129473936-129479174:+ ENST00000543953.5_1_X:129473936-129479174:+ SLC25A14 5' Overlap uORF 5' Overlap uORF GN=SLC25A14 5' Overlap uORF E_20221201_NO30_400nL_HLAc1_4e7_PDAC_Slot2-6_1_3524 SCP E_20221201_NO30_400nL_HLAc1_4e7_PDAC_Slot2-6_1_3524.7600.1554.2 2 1 1 8.78 6.17 3.76 2.61 60.5 7 1 1 6310.0 Hydrogen 120 (N) 9 SPSTSRVAL (Q) 9 SPLRSSSAL S/P/S/T/S R\V\A\L 77.8 7 3 4 0 0 unbiased 26.258518 0.779665 459.2537 917.505 -0.0043 -4.7 -2.3000000000000003 9.47 15805.9 nuORFdb_human ENST00000612689.1_1_19:10444887-10445313:+ ENST00000612689.1_1_19:10444887-10445313:+ AC114271.2 Antisense lncRNA GN=AC114271.2 Antisense E_20221201_NO30_400nL_HLAc1_4e7_PDAC_Slot2-6_1_3524 Exploris_FAIMS VH20221207_MKP_FAIMS2022E_HLAc1_4e7_PDAC.22446.22446.2 2 1 1 12.46 10.3 2.58 2.16 82.8 7 1 1 2510000.0 Hydrogen 120 (N) 9 SPSTSRVAL (Q) 9 SPSARVSTI S/P|S|T/S R\V/A|L 77.8 7 4 6 3 0 dual b/y bias 0.997 100.0 46.68 120.0 -70.0 459.2553 917.505 -0.0017 -1.9 0.7000000000000002 9.47 15805.9 nuORFdb_human ENST00000612689.1_1_19:10444887-10445313:+ ENST00000612689.1_1_19:10444887-10445313:+ AC114271.2 Antisense lncRNA GN=AC114271.2 Antisense VH20221207_MKP_FAIMS2022E_HLAc1_4e7_PDAC Eva-50mio-3fr AL20210827_EV_HLA_PDAC_PANFRO413_2D_50e6_W632_classI_fxn1.22935.22935.1 1 1 1 12.27 6.85 6.63 5.41 85.6 6 0 0 1770000.0 Hydrogen 5 (N) 9 SPTEVAGTF (L) 9 ETPSVKmT S P\T\E\V\A\G\T F 55.6 5 6 0 0 0 b-ion bias 0.937 74.3 42.38 100.0 0.0 908.4361 908.436 0.0001 0.1 0.0 4.0 3407.8 nuORFdb_human ENST00000466942.2_1_10:77167305-77167401:+ ENST00000466942.2_1_10:77167305-77167401:+ ZNF503-AS2 Antisense lncRNA GN=ZNF503-AS2 Antisense AL20210827_EV_HLA_PDAC_PANFRO413_2D_50e6_W632_classI_fxn1 SCP E_20221201_NO30_400nL_HLAc1_4e7_PDAC_Slot2-6_1_3524.14149.562.1 1 2 1 12.9 7.29 5.95 5.6 90.3 5 0 0 43500.0 Hydrogen 5 (N) 9 SPTEVAGTF (L) 9 AEPSTVGTF S P T E|V\A\G\T\F 55.6 5 5 1 1 0 b-ion bias 48.880329 1.38873 908.434 908.436 -0.002 -2.2 0.19999999999999973 4.0 3407.8 nuORFdb_human ENST00000466942.2_1_10:77167305-77167401:+ ENST00000466942.2_1_10:77167305-77167401:+ ZNF503-AS2 Antisense lncRNA GN=ZNF503-AS2 Antisense E_20221201_NO30_400nL_HLAc1_4e7_PDAC_Slot2-6_1_3524 SCP E_20221201_NO30_400nL_HLAc1_4e7_PDAC_Slot2-6_1_3524.20265.557.1 1 1 1 7.62 4.1 2.4 3.52 65.9 5 0 0 13600.0 Hydrogen 23 (T) 9 SPTLNVSAL (-) 9 VDLGNISAL S P T L|N\V\S\A\L 55.6 5 5 1 1 0 b-ion bias 70.007919 1.39227 901.4951 901.499 -0.0039 -4.4 -2.0000000000000004 5.24 3558.1 nuORFdb_human ENST00000558841.1_1_15:52338161-52338257:+ ENST00000558841.1_1_15:52338161-52338257:+ MAPK6 ncRNA Processed Transcript lncRNA GN=MAPK6 ncRNA Processed Transcript E_20221201_NO30_400nL_HLAc1_4e7_PDAC_Slot2-6_1_3524 Eva-50mio-3fr AL20210827_EV_HLA_PDAC_PANFRO413_2D_50e6_W632_classI_fxn3.45624.45624.1 1 1 1 8.84 9.04 8.84 64.4 7 0 0 714000.0 Hydrogen 3 (Q) 9 SPVSGTWLL (-) 9 S P\V\S\G\T\W\L-L 77.8 7 6 0 0 1 b-ion bias 0.999 45.0 82.4 100.0 0.0 959.5209 959.52 0.0012 1.3 0.8 5.24 1218.4 nuORFdb_human ENST00000264864.7_1_4:25235715-25235748:+ ENST00000264864.7_1_4:25235715-25235748:+ PI4K2B 5' uORF 5' uORF GN=PI4K2B 5' uORF AL20210827_EV_HLA_PDAC_PANFRO413_2D_50e6_W632_classI_fxn3 Exploris AL20221201_MKP_PDAC_01.59430.59559.1 1 2 1 10.35 -0.22 9.99 10.57 73.2 7 0 0 107000000.0 Hydrogen 3 (Q) 9 SPVSGTWLL (-) 9 PSVSIACLL S P\V\S\G\T\W\L-L 77.8 7 6 0 0 1 b-ion bias 0.997 100.0 90.12 100.0 0.0 959.5191 959.52 -0.0005 -0.6 -1.4 5.24 1218.4 nuORFdb_human ENST00000264864.7_1_4:25235715-25235748:+ ENST00000264864.7_1_4:25235715-25235748:+ PI4K2B 5' uORF 5' uORF GN=PI4K2B 5' uORF AL20221201_MKP_PDAC_01 SCP E_20221201_NO30_400nL_HLAc1_4e7_PDAC_Slot2-6_1_3524.26743.450.1 1 3 1 10.61 4.47 5.49 6.14 83.1 6 0 0 129000.0 Hydrogen 3 (Q) 9 SPVSGTWLL (-) 9 LEQASLWL S P V/S\G\T|W\L\L 66.7 6 5 2 1 0 b-ion bias 92.390701 1.455487 959.5167 959.52 -0.0027 -2.8 -0.3999999999999999 5.24 1218.4 nuORFdb_human ENST00000264864.7_1_4:25235715-25235748:+ ENST00000264864.7_1_4:25235715-25235748:+ PI4K2B 5' uORF 5' uORF GN=PI4K2B 5' uORF E_20221201_NO30_400nL_HLAc1_4e7_PDAC_Slot2-6_1_3524 Exploris_FAIMS VH20221207_MKP_FAIMS2022E_HLAc1_4e7_PDAC.61770.61770.2 2 1 1 10.41 5.92 3.68 4.48 74.0 6 0 0 7170000.0 Hydrogen 3 (Q) 9 SPVSGTWLL (-) 9 ASEAGLWII S P\V\S\G T\W|L/L 55.6 5 5 2 1 0 b-ion bias 1.0 96.8 106.37 23.46 -70.0 480.2621 959.52 -0.0027 -2.8 -0.19999999999999973 5.24 1218.4 nuORFdb_human ENST00000264864.7_1_4:25235715-25235748:+ ENST00000264864.7_1_4:25235715-25235748:+ PI4K2B 5' uORF 5' uORF GN=PI4K2B 5' uORF VH20221207_MKP_FAIMS2022E_HLAc1_4e7_PDAC Eva-50mio-3fr AL20210827_EV_HLA_PDAC_PANFRO413_2D_50e6_W632_classI_fxn2.24839.24839.2 2 1 1 16.18 6.34 10.95 9.84 85.5 9 1 1 2960000.0 Hydrogen 23 (S) 11 SPWVGTEKGTF (R) 11 SPWEKRmMK S/P|W|V|G/T/E/K G-T/F 81.8 9 3 8 3 1 y-ion bias 0.47 85.9 46.37 100.0 0.0 604.8016 1208.595 0.0013 1.0 0.7 5.72 5734.4 nuORFdb_human ENST00000484003.1_1_6:32810760-32812105:- ENST00000484003.1_1_6:32810760-32812105:- PSMB8 ncRNA Retained Intron lncRNA GN=PSMB8 ncRNA Retained Intron AL20210827_EV_HLA_PDAC_PANFRO413_2D_50e6_W632_classI_fxn2 Exploris AL20221201_MKP_PDAC_01.32704.32704.2 2 1 1 9.9 9.9 9.9 62.9 5 1 1 13400000.0 Hydrogen 23 (S) 11 SPWVGTEKGTF (R) 11 S P|W|V/G-T E/K G T/F 36.4 4 2 5 2 1 y-ion bias 0.855 100.0 51.87 45.02 0.0 604.8014 1208.595 0.0009 0.8 0.0 5.72 5734.4 nuORFdb_human ENST00000484003.1_1_6:32810760-32812105:- ENST00000484003.1_1_6:32810760-32812105:- PSMB8 ncRNA Retained Intron lncRNA GN=PSMB8 ncRNA Retained Intron AL20221201_MKP_PDAC_01 SCP E_20221201_NO30_400nL_HLAc1_4e7_PDAC_Slot2-6_1_3524.14962.1288.2 2 1 1 13.24 3.16 8.17 10.08 78.0 9 1 1 18900.0 Hydrogen 23 (S) 11 SPWVGTEKGTF (R) 11 SPERAGYASASL S/P/W|V|G|T\E/K\G T\F 81.8 9 6 6 3 0 unbiased 51.690559 0.946199 604.7996 1208.595 -0.0019 -1.6 0.7999999999999998 5.72 5734.4 nuORFdb_human ENST00000484003.1_1_6:32810760-32812105:- ENST00000484003.1_1_6:32810760-32812105:- PSMB8 ncRNA Retained Intron lncRNA GN=PSMB8 ncRNA Retained Intron E_20221201_NO30_400nL_HLAc1_4e7_PDAC_Slot2-6_1_3524 10mio-rep1 E_20221201_NO30_400nL_HLAc1_1e7_directIP_titration_rep1_Slot2-3_1_3512.25703.403.1 1 3 1 14.54 8.78 6.57 5.76 91.8 7 0 0 8710.0 Hydrogen 104 (K) 9 SQDPIIFSV (G) 9 nGGTPLLYAV S Q/D|P\I\I\F\S\V 77.8 7 6 2 1 0 b-ion bias 88.780792 1.483371 1005.5163 1005.525 -0.0051 -5.1 2.6000000000000005 3.8 27829.3 smORF_v4|nuORFdb_human BMS1P14_9:67334095-67328440:- BMS1P14_9:67334095-67328440:-|ENST00000416720.1_9:42808985-42809460:+|ENST00000443754.1_1_9:67328347-67334199:- BMS1P14 Pseudogene 9:67334095-67328440:- 9 BMS1P14 Pseudogene Pseudogene GN=BMS1P14 E_20221201_NO30_400nL_HLAc1_1e7_directIP_titration_rep1_Slot2-3_1_3512 10mio-rep2 E_20221201_NO30_400nL_HLAc1_1e7_directIP_titration_rep2_Slot2-3_1_3513.25630.407.1 1 2 1 12.76 8.16 5.25 4.6 85.0 6 0 0 3450.0 Hydrogen 104 (K) 9 SQDPIIFSV (G) 9 nGGTPLLYAV S Q D|P\I|I\F\S\V 66.7 6 6 2 2 0 b-ion bias 88.521202 1.481164 1005.5151 1005.525 -0.0051 -5.1 1.6000000000000005 3.8 27829.3 smORF_v4|nuORFdb_human BMS1P14_9:67334095-67328440:- BMS1P14_9:67334095-67328440:-|ENST00000416720.1_9:42808985-42809460:+|ENST00000443754.1_1_9:67328347-67334199:- BMS1P14 Pseudogene 9:67334095-67328440:- 9 BMS1P14 Pseudogene Pseudogene GN=BMS1P14 E_20221201_NO30_400nL_HLAc1_1e7_directIP_titration_rep2_Slot2-3_1_3513 10mio-rep3 E_20221201_NO30_400nL_HLAc1_1e7_directIP_titration_rep3_Slot2-3_1_3514.25587.409.1 1 2 1 13.58 7.7 6.13 5.88 88.3 7 0 0 4100.0 Hydrogen 104 (K) 9 SQDPIIFSV (G) 9 nGGTPLLYAV S Q/D|P\I\I\F\S\V 77.8 7 6 2 1 0 b-ion bias 88.378906 1.479868 1005.5184 1005.525 -0.0051 -5.1 1.2000000000000002 3.8 27829.3 smORF_v4|nuORFdb_human BMS1P14_9:67334095-67328440:- BMS1P14_9:67334095-67328440:-|ENST00000416720.1_9:42808985-42809460:+|ENST00000443754.1_1_9:67328347-67334199:- BMS1P14 Pseudogene 9:67334095-67328440:- 9 BMS1P14 Pseudogene Pseudogene GN=BMS1P14 E_20221201_NO30_400nL_HLAc1_1e7_directIP_titration_rep3_Slot2-3_1_3514 20mio-rep1 E_20221201_NO30_400nL_HLAc1_2e7_directIP_titration_rep1_Slot2-4_1_3515.25581.400.1 1 3 1 14.25 7.75 6.4 6.5 91.0 6 0 0 9850.0 Hydrogen 104 (K) 9 SQDPIIFSV (G) 9 nGGTPLLYAV S Q/D|P I|I\F\S\V 55.6 5 5 3 2 0 unbiased 88.33654 1.4851 1005.5198 1005.525 -0.0051 -5.1 1.1000000000000005 3.8 27829.3 smORF_v4|nuORFdb_human BMS1P14_9:67334095-67328440:- BMS1P14_9:67334095-67328440:-|ENST00000416720.1_9:42808985-42809460:+|ENST00000443754.1_1_9:67328347-67334199:- BMS1P14 Pseudogene 9:67334095-67328440:- 9 BMS1P14 Pseudogene Pseudogene GN=BMS1P14 E_20221201_NO30_400nL_HLAc1_2e7_directIP_titration_rep1_Slot2-4_1_3515 20mio-rep2 E_20221201_NO30_400nL_HLAc1_2e7_directIP_titration_rep3_Slot2-4_1_3518.25617.1505.2 2 4 1 15.39 10.33 3.71 5.06 93.0 6 0 0 9320.0 Hydrogen 104 (K) 9 SQDPIIFSV (G) 9 qETAKLFSV S Q/D\P I|I|F\S\V 55.6 5 5 3 2 0 unbiased 88.479736 0.810201 503.2617 1005.525 -0.0084 -8.4 -2.1000000000000005 3.8 27829.3 smORF_v4|nuORFdb_human BMS1P14_9:67334095-67328440:- BMS1P14_9:67334095-67328440:-|ENST00000416720.1_9:42808985-42809460:+|ENST00000443754.1_1_9:67328347-67334199:- BMS1P14 Pseudogene 9:67334095-67328440:- 9 BMS1P14 Pseudogene Pseudogene GN=BMS1P14 E_20221201_NO30_400nL_HLAc1_2e7_directIP_titration_rep3_Slot2-4_1_3518 20mio-rep3 E_20221201_NO30_400nL_HLAc1_2e7_directIP_titration_rep4_Slot2-4_1_3519.25640.394.1 1 3 1 14.19 8.37 6.11 5.81 91.3 7 0 0 9060.0 Hydrogen 104 (K) 9 SQDPIIFSV (G) 9 nGGTPLLYAV S Q/D|P\I|I\F\S\V 77.8 7 6 3 2 0 b-ion bias 88.552818 1.488619 1005.5193 1005.525 -0.0051 -5.1 0.7000000000000002 3.8 27829.3 smORF_v4|nuORFdb_human BMS1P14_9:67334095-67328440:- BMS1P14_9:67334095-67328440:-|ENST00000416720.1_9:42808985-42809460:+|ENST00000443754.1_1_9:67328347-67334199:- BMS1P14 Pseudogene 9:67334095-67328440:- 9 BMS1P14 Pseudogene Pseudogene GN=BMS1P14 E_20221201_NO30_400nL_HLAc1_2e7_directIP_titration_rep4_Slot2-4_1_3519 40mio-rep1 E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep1_Slot1-04_1_3496.25821.369.1 1 3 1 14.83 8.51 7.2 6.32 91.4 7 0 0 24900.0 Hydrogen 104 (K) 9 SQDPIIFSV (G) 9 nGGTPLLYAV S Q/D|P\I\I\F\S\V 77.8 7 6 2 1 0 b-ion bias 89.20063 1.503869 1005.523 1005.525 -0.0051 -5.1 -1.8999999999999995 3.8 27829.3 smORF_v4|nuORFdb_human BMS1P14_9:67334095-67328440:- BMS1P14_9:67334095-67328440:-|ENST00000416720.1_9:42808985-42809460:+|ENST00000443754.1_1_9:67328347-67334199:- BMS1P14 Pseudogene 9:67334095-67328440:- 9 BMS1P14 Pseudogene Pseudogene GN=BMS1P14 E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep1_Slot1-04_1_3496 40mio-rep2 E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep2_Slot2-5_1_3520.25587.388.1 1 2 1 15.23 8.26 6.42 6.97 92.9 7 0 0 12300.0 Hydrogen 104 (K) 9 SQDPIIFSV (G) 9 nGGTPLLYAV S Q/D|P\I|I\F\S\V 77.8 7 6 3 2 0 b-ion bias 88.388641 1.492615 1005.5211 1005.525 -0.0051 -5.1 -0.1999999999999993 3.8 27829.3 smORF_v4|nuORFdb_human BMS1P14_9:67334095-67328440:- BMS1P14_9:67334095-67328440:-|ENST00000416720.1_9:42808985-42809460:+|ENST00000443754.1_1_9:67328347-67334199:- BMS1P14 Pseudogene 9:67334095-67328440:- 9 BMS1P14 Pseudogene Pseudogene GN=BMS1P14 E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep2_Slot2-5_1_3520 40mio-rep3 E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep3_Slot2-5_1_3521.25590.392.1 1 1 1 12.05 7.19 4.7 4.86 81.1 6 0 0 6060.0 Hydrogen 104 (K) 9 SQDPIIFSV (G) 9 nGGTPLLYAV S Q D|P\I|I\F\S\V 66.7 6 6 2 2 0 b-ion bias 88.397102 1.490177 1005.5218 1005.525 -0.0051 -5.1 -0.5 3.8 27829.3 smORF_v4|nuORFdb_human BMS1P14_9:67334095-67328440:- BMS1P14_9:67334095-67328440:-|ENST00000416720.1_9:42808985-42809460:+|ENST00000443754.1_1_9:67328347-67334199:- BMS1P14 Pseudogene 9:67334095-67328440:- 9 BMS1P14 Pseudogene Pseudogene GN=BMS1P14 E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep3_Slot2-5_1_3521 40mio-rep4 E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep4_Slot2-5_1_3522.25602.390.1 1 3 1 14.56 8.85 5.63 5.72 89.4 7 0 0 12700.0 Hydrogen 104 (K) 9 SQDPIIFSV (G) 9 nGGTPLLYAV S Q/D|P\I|I\F\S\V 77.8 7 6 3 2 0 b-ion bias 88.429741 1.491516 1005.522 1005.525 -0.0051 -5.1 -0.39999999999999947 3.8 27829.3 smORF_v4|nuORFdb_human BMS1P14_9:67334095-67328440:- BMS1P14_9:67334095-67328440:-|ENST00000416720.1_9:42808985-42809460:+|ENST00000443754.1_1_9:67328347-67334199:- BMS1P14 Pseudogene 9:67334095-67328440:- 9 BMS1P14 Pseudogene Pseudogene GN=BMS1P14 E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep4_Slot2-5_1_3522 Eva-50mio-3fr AL20210827_EV_HLA_PDAC_PANFRO413_2D_50e6_W632_classI_fxn2.18110.18110.2 2 1 1 11.92 5.03 2.69 6.9 78.3 5 1 1 26100000.0 Hydrogen 66 (N) 9 SRVNGAWQY (E) 9 GEKRGGWFSG S R V N\G\A\W|Q|Y 55.6 5 5 2 2 0 b-ion bias 0.998 100.0 34.7 37.0 0.0 540.7643 1080.522 -0.0008 -0.7 -1.0 8.46 17668.9 nuORFdb_human ENST00000412609.5_1_19:40170059-40174276:+ ENST00000412609.5_1_19:40170059-40174276:+|ENST00000598164.2_1_19:40172096-40174784:+ LGALS17A Pseudogene Pseudogene GN=LGALS17A Pseudogene AL20210827_EV_HLA_PDAC_PANFRO413_2D_50e6_W632_classI_fxn2 Exploris_FAIMS VH20221207_MKP_FAIMS2022E_HLAc1_4e7_PDAC.28920.28920.2 2 1 1 10.6 4.48 3.56 6.12 70.0 6 1 1 131000000.0 Hydrogen 66 (N) 9 SRVNGAWQY (E) 9 GEKRGGWFSG S R\V N\G\A\W|Q/Y 55.6 5 5 2 1 0 b-ion bias 0.936 97.5 57.15 1.92 -50.0 540.7632 1080.522 -0.0029 -2.7 -0.10000000000000009 8.46 17668.9 nuORFdb_human ENST00000412609.5_1_19:40170059-40174276:+ ENST00000412609.5_1_19:40170059-40174276:+|ENST00000598164.2_1_19:40172096-40174784:+ LGALS17A Pseudogene Pseudogene GN=LGALS17A Pseudogene VH20221207_MKP_FAIMS2022E_HLAc1_4e7_PDAC 40mio-rep1 E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep1_Slot1-04_1_3496.9681.1522.2 2 1 1 10.35 3.94 6.16 6.41 73.0 7 0 0 7000.0 Hydrogen 2 (I) 9 SSAPAHAPW (Y) 9 SSARMSDAV S/S/A/P/A/H A|P\W 77.8 7 2 6 1 0 y-ion bias 33.445313 0.799535 462.2191 923.437 -0.0063 -6.8 -3.5999999999999996 6.46 3924.4 nuORFdb_human T207413_2:218088592-218088691:+ T207413_2:218088592-218088691:+ G048002 lincRNA lncRNA GN=G048002 lincRNA E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep1_Slot1-04_1_3496 40mio-rep3 E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep3_Slot2-5_1_3521.9406.1544.2 2 1 1 10.42 6.05 4.29 4.36 70.6 6 0 0 3930.0 Hydrogen 2 (I) 9 SSAPAHAPW (Y) 9 SSACLSLW S/S/A|P/A H A|P\W 66.7 6 3 5 2 0 unbiased 32.496529 0.785838 462.2192 923.437 -0.0063 -6.8 -2.2 6.46 3924.4 nuORFdb_human T207413_2:218088592-218088691:+ T207413_2:218088592-218088691:+ G048002 lincRNA lncRNA GN=G048002 lincRNA E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep3_Slot2-5_1_3521 40mio-rep4 E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep4_Slot2-5_1_3522.9406.1531.2 2 2 1 10.02 5.94 4.43 4.08 77.3 6 0 0 8830.0 Hydrogen 2 (I) 9 SSAPAHAPW (Y) 9 SSACLSLW S/S/A/P A H/A|P\W 66.7 6 2 5 1 0 y-ion bias 32.485981 0.794113 462.2201 923.437 -0.0043 -4.6 0.10000000000000053 6.46 3924.4 nuORFdb_human T207413_2:218088592-218088691:+ T207413_2:218088592-218088691:+ G048002 lincRNA lncRNA GN=G048002 lincRNA E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep4_Slot2-5_1_3522 20mio-rep3 E_20221201_NO30_400nL_HLAc1_2e7_directIP_titration_rep4_Slot2-4_1_3519.21066.397.1 1 1 1 10.35 6.16 6.42 4.19 78.1 6 0 0 1690.0 Hydrogen 69 (N) 10 SSDPSLIGVY (S) 10 VDGnSLIVGY S S D/P S|L\I\G\V\Y 50.0 5 5 2 1 0 b-ion bias 72.758141 1.487239 1037.5094 1037.515 -0.005 -4.8 1.0 3.8 9020.1 nuORFdb_human ENST00000475700.1_1_1:112303127-112303484:+ ENST00000475700.1_1_1:112303127-112303484:+ DDX20 3' Overlap dORF 3' Overlap dORF GN=DDX20 3' Overlap dORF E_20221201_NO30_400nL_HLAc1_2e7_directIP_titration_rep4_Slot2-4_1_3519 40mio-rep1 E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep1_Slot1-04_1_3496.21220.379.1 1 1 1 10.01 6.1 6.95 3.92 78.6 6 0 0 3770.0 Hydrogen 69 (N) 10 SSDPSLIGVY (S) 10 VDGnSLIVGY S S D/P S\L|I\G\V\Y 50.0 5 5 2 1 0 b-ion bias 73.304474 1.497882 1037.5112 1037.515 -0.005 -4.8 -1.5999999999999996 3.8 9020.1 nuORFdb_human ENST00000475700.1_1_1:112303127-112303484:+ ENST00000475700.1_1_1:112303127-112303484:+ DDX20 3' Overlap dORF 3' Overlap dORF GN=DDX20 3' Overlap dORF E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep1_Slot1-04_1_3496 40mio-rep4 E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep4_Slot2-5_1_3522.20955.391.1 1 2 1 10.4 6.02 6.52 4.37 76.6 6 0 0 5580.0 Hydrogen 69 (N) 10 SSDPSLIGVY (S) 10 VDGnSLIVGY S S D/P S/L\I\G\V\Y 50.0 5 4 2 0 0 b-ion bias 72.371246 1.490729 1037.5078 1037.515 -0.005 -4.8 -0.09999999999999964 3.8 9020.1 nuORFdb_human ENST00000475700.1_1_1:112303127-112303484:+ ENST00000475700.1_1_1:112303127-112303484:+ DDX20 3' Overlap dORF 3' Overlap dORF GN=DDX20 3' Overlap dORF E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep4_Slot2-5_1_3522 40mio-rep2 E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep2_Slot2-5_1_3520.6491.1563.2 2 1 1 12.36 8.22 4.68 4.13 79.2 7 1 1 3970.0 Hydrogen 47 (F) 9 SSGPTRAIP (C) 9 SSRPASALP S S/G/P/T/R\A\I\P 77.8 7 3 4 0 0 unbiased 22.429991 0.773889 443.2385 885.479 -0.0081 -9.2 -4.299999999999999 9.47 6797.7 nuORFdb_human ENST00000317459.10_1_11:63992170-63992442:- ENST00000317459.10_1_11:63992170-63992442:- TRPT1 Out-of-Frame Out-of-Frame GN=TRPT1 Out-of-Frame E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep2_Slot2-5_1_3520 20mio-rep2 E_20221201_NO30_400nL_HLAc1_2e7_directIP_titration_rep3_Slot2-4_1_3518.25311.491.1 1 1 1 11.27 6.73 6.88 4.54 86.1 7 0 0 3120.0 Hydrogen 4 (T) 9 SSLPVNGLW (R) 9 SSLGNVPLAD S S/L/P\V\N\G\L\W 77.8 7 5 2 0 0 b-ion bias 87.423615 1.431594 972.5048 972.515 -0.0099 -10.2 -3.8999999999999995 5.24 5278.0 nuORFdb_human ENST00000313608.12_1_17:55918309-55918571:- ENST00000313608.12_1_17:55918309-55918571:- MRPS23 Out-of-Frame Out-of-Frame GN=MRPS23 Out-of-Frame E_20221201_NO30_400nL_HLAc1_2e7_directIP_titration_rep3_Slot2-4_1_3518 20mio-rep3 E_20221201_NO30_400nL_HLAc1_2e7_directIP_titration_rep4_Slot2-4_1_3519.25351.491.1 1 2 1 12.45 4.57 8.17 7.87 87.0 7 0 0 6330.0 Hydrogen 4 (T) 9 SSLPVNGLW (R) 9 SSLGNVPLAD S S/L/P\V|N\G\L\W 77.8 7 5 3 1 0 unbiased 87.552437 1.431121 972.5113 972.515 -0.0039 -4.0 1.7999999999999998 5.24 5278.0 nuORFdb_human ENST00000313608.12_1_17:55918309-55918571:- ENST00000313608.12_1_17:55918309-55918571:- MRPS23 Out-of-Frame Out-of-Frame GN=MRPS23 Out-of-Frame E_20221201_NO30_400nL_HLAc1_2e7_directIP_titration_rep4_Slot2-4_1_3519 40mio-rep1 E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep1_Slot1-04_1_3496.25535.473.1 1 3 1 12.65 3.61 7.79 9.05 86.3 7 0 0 28900.0 Hydrogen 4 (T) 9 SSLPVNGLW (R) 9 SSLGNVPLAD S S/L/P\V|N\G\L\W 77.8 7 5 3 1 0 unbiased 88.213142 1.442307 972.5107 972.515 -0.0039 -4.0 -0.7999999999999998 5.24 5278.0 nuORFdb_human ENST00000313608.12_1_17:55918309-55918571:- ENST00000313608.12_1_17:55918309-55918571:- MRPS23 Out-of-Frame Out-of-Frame GN=MRPS23 Out-of-Frame E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep1_Slot1-04_1_3496 40mio-rep2 E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep2_Slot2-5_1_3520.25301.490.1 1 2 1 11.84 4.15 7.7 7.69 85.6 7 0 0 14100.0 Hydrogen 4 (T) 9 SSLPVNGLW (R) 9 SSLGNVPLAD S S/L/P\V|N\G\L\W 77.8 7 5 3 1 0 unbiased 87.400856 1.431838 972.5069 972.515 -0.0079 -8.1 -3.1999999999999993 5.24 5278.0 nuORFdb_human ENST00000313608.12_1_17:55918309-55918571:- ENST00000313608.12_1_17:55918309-55918571:- MRPS23 Out-of-Frame Out-of-Frame GN=MRPS23 Out-of-Frame E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep2_Slot2-5_1_3520 40mio-rep3 E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep3_Slot2-5_1_3521.25314.487.1 1 1 1 10.09 2.46 7.0 7.62 82.8 7 0 0 9220.0 Hydrogen 4 (T) 9 SSLPVNGLW (R) 9 MRAPSKPW S S/L/P\V/N\G\L\W 77.8 7 4 3 0 0 unbiased 87.444344 1.433507 972.511 972.515 -0.0039 -4.0 0.5999999999999996 5.24 5278.0 nuORFdb_human ENST00000313608.12_1_17:55918309-55918571:- ENST00000313608.12_1_17:55918309-55918571:- MRPS23 Out-of-Frame Out-of-Frame GN=MRPS23 Out-of-Frame E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep3_Slot2-5_1_3521 40mio-rep4 E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep4_Slot2-5_1_3522.25309.482.1 1 1 1 13.12 4.65 8.74 8.47 84.9 7 0 0 3070.0 Hydrogen 4 (T) 9 SSLPVNGLW (R) 9 SSLGNVPLAD S S/L/P\V|N\G\L\W 77.8 7 5 3 1 0 unbiased 87.417404 1.436482 972.5099 972.515 -0.0049 -5.0 -0.2999999999999998 5.24 5278.0 nuORFdb_human ENST00000313608.12_1_17:55918309-55918571:- ENST00000313608.12_1_17:55918309-55918571:- MRPS23 Out-of-Frame Out-of-Frame GN=MRPS23 Out-of-Frame E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep4_Slot2-5_1_3522 40mio-rep1 E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep1_Slot1-04_1_3496.11144.1562.2 2 2 1 11.44 4.77 4.48 6.67 78.6 7 0 1 19600.0 Hydrogen 14 (R) 9 SSLRPGAGW (R) 9 SSPLAGGVGW S/S/L/R/P G\A|G\W 77.8 7 3 5 1 0 unbiased 38.496574 0.774581 465.7418 930.479 -0.0025 -2.7 0.5 9.47 2615.0 nuORFdb_human ENST00000307712.10_1_14:39572361-39572439:- ENST00000307712.10_1_14:39572361-39572439:- SEC23A 5' uORF 5' uORF GN=SEC23A 5' uORF E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep1_Slot1-04_1_3496 40mio-rep2 E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep2_Slot2-5_1_3520.10844.1581.2 2 1 1 9.08 2.19 3.83 6.89 80.4 6 0 1 7720.0 Hydrogen 14 (R) 9 SSLRPGAGW (R) 9 AAGALREGW S S/L/R/P G\A|G\W 55.6 5 3 4 1 0 unbiased 37.461899 0.762167 465.7403 930.479 -0.0065 -7.0 -2.0999999999999996 9.47 2615.0 nuORFdb_human ENST00000307712.10_1_14:39572361-39572439:- ENST00000307712.10_1_14:39572361-39572439:- SEC23A 5' uORF 5' uORF GN=SEC23A 5' uORF E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep2_Slot2-5_1_3520 40mio-rep3 E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep3_Slot2-5_1_3521.10862.1580.2 2 2 1 7.96 2.84 3.17 5.11 76.4 7 0 1 16300.0 Hydrogen 14 (R) 9 SSLRPGAGW (R) 9 SSPLAGGVGW S/S/L/R/P G\A\G\W 77.8 7 3 4 0 0 unbiased 37.52668 0.763099 465.7394 930.479 -0.0085 -9.1 -4.5 9.47 2615.0 nuORFdb_human ENST00000307712.10_1_14:39572361-39572439:- ENST00000307712.10_1_14:39572361-39572439:- SEC23A 5' uORF 5' uORF GN=SEC23A 5' uORF E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep3_Slot2-5_1_3521 40mio-rep4 E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep4_Slot2-5_1_3522.10853.1579.2 2 1 1 8.55 4.7 1.58 3.86 77.7 6 0 1 8130.0 Hydrogen 14 (R) 9 SSLRPGAGW (R) 9 SSPLAGGVGW S/S/L/R/P G A|G\W 66.7 6 2 5 1 0 y-ion bias 37.481052 0.763635 465.7399 930.479 -0.0065 -7.0 -2.3 9.47 2615.0 nuORFdb_human ENST00000307712.10_1_14:39572361-39572439:- ENST00000307712.10_1_14:39572361-39572439:- SEC23A 5' uORF 5' uORF GN=SEC23A 5' uORF E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep4_Slot2-5_1_3522 20mio-rep1 E_20221201_NO30_400nL_HLAc1_2e7_directIP_titration_rep1_Slot2-4_1_3515.9101.1427.2 2 1 1 9.0 3.68 7.8 5.31 56.7 8 1 1 5960.0 M6m Hydrogen 4 (L) 9 SSmSLQRTW (-) 9 SSmLSSVHFT S/S/m|S/L|Q/R\T\W 100.0 9 4 6 2 0 unbiased m:Oxidized methionine 31.438068 0.859578 556.2631 1095.525 15.9936 -1.2 5.0 9.47 1426.7 nuORFdb_human ENST00000288607.2_1_7:1607452-1608764:- ENST00000288607.2_1_7:1607452-1608764:- PSMG3 Out-of-Frame Out-of-Frame GN=PSMG3 Out-of-Frame E_20221201_NO30_400nL_HLAc1_2e7_directIP_titration_rep1_Slot2-4_1_3515 20mio-rep3 E_20221201_NO30_400nL_HLAc1_2e7_directIP_titration_rep4_Slot2-4_1_3519.9135.1423.2 2 1 1 10.15 5.59 10.15 65.6 7 1 1 4800.0 M6m Hydrogen 4 (L) 9 SSmSLQRTW (-) 9 S S/m|S/L|Q|R|T\W 77.8 7 5 6 4 0 dual b/y bias m:Oxidized methionine 31.558207 0.861665 556.2599 1095.525 15.9876 -6.6 -0.7999999999999998 9.47 1426.7 nuORFdb_human ENST00000288607.2_1_7:1607452-1608764:- ENST00000288607.2_1_7:1607452-1608764:- PSMG3 Out-of-Frame Out-of-Frame GN=PSMG3 Out-of-Frame E_20221201_NO30_400nL_HLAc1_2e7_directIP_titration_rep4_Slot2-4_1_3519 40mio-rep1 E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep1_Slot1-04_1_3496.9384.1379.2 2 2 1 13.1 2.14 8.07 10.97 73.8 6 1 1 18700.0 M6m Hydrogen 4 (L) 9 SSmSLQRTW (-) 9 QSFARVmEQ S S/m|S|L|Q/R T\W 55.6 5 4 5 3 0 dual b/y bias m:Oxidized methionine 32.419445 0.88921 556.2614 1095.525 15.9896 -4.8 -1.5999999999999996 9.47 1426.7 nuORFdb_human ENST00000288607.2_1_7:1607452-1608764:- ENST00000288607.2_1_7:1607452-1608764:- PSMG3 Out-of-Frame Out-of-Frame GN=PSMG3 Out-of-Frame E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep1_Slot1-04_1_3496 40mio-rep4 E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep4_Slot2-5_1_3522.9118.1406.2 2 2 1 12.2 12.2 12.2 67.1 7 1 1 16200.0 M6m Hydrogen 4 (L) 9 SSmSLQRTW (-) 9 S S/m/S|L|Q/R\T\W 77.8 7 4 5 2 0 unbiased m:Oxidized methionine 31.491968 0.872842 556.2596 1095.525 15.9876 -6.6 -1.8999999999999995 9.47 1426.7 nuORFdb_human ENST00000288607.2_1_7:1607452-1608764:- ENST00000288607.2_1_7:1607452-1608764:- PSMG3 Out-of-Frame Out-of-Frame GN=PSMG3 Out-of-Frame E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep4_Slot2-5_1_3522 10mio-rep3 E_20221201_NO30_400nL_HLAc1_1e7_directIP_titration_rep3_Slot2-3_1_3514.18250.1351.2 2 2 1 7.78 2.46 5.1 5.32 62.4 6 0 1 6360.0 Hydrogen 11 (M) 11 SSTKPGQLQLW (V) 11 WLSKKHmIW S S/T K|P G Q\L/Q|L\W 36.4 4 4 4 2 0 unbiased 63.042095 0.907069 622.8303 1244.663 -0.0106 -8.5 -2.2 8.47 3865.6 nuORFdb_human ENST00000488086.1_1_2:74432953-74433055:+ ENST00000488086.1_1_2:74432953-74433055:+|ENST00000394053.6_1_2:74432953-74434854:+ MTHFD2 ncRNA Processed Transcript lncRNA GN=MTHFD2 ncRNA Processed Transcript E_20221201_NO30_400nL_HLAc1_1e7_directIP_titration_rep3_Slot2-3_1_3514 20mio-rep1 E_20221201_NO30_400nL_HLAc1_2e7_directIP_titration_rep1_Slot2-4_1_3515.18270.1342.2 2 1 1 11.59 4.46 6.66 7.13 76.1 6 0 1 5290.0 Hydrogen 11 (M) 11 SSTKPGQLQLW (V) 11 QCIQRLQLW S S/T K|P G Q\L|Q|L\W 36.4 4 5 4 3 0 unbiased 63.096798 0.912707 622.8311 1244.663 -0.0086 -6.9 -0.7000000000000002 8.47 3865.6 nuORFdb_human ENST00000488086.1_1_2:74432953-74433055:+ ENST00000488086.1_1_2:74432953-74433055:+|ENST00000394053.6_1_2:74432953-74434854:+ MTHFD2 ncRNA Processed Transcript lncRNA GN=MTHFD2 ncRNA Processed Transcript E_20221201_NO30_400nL_HLAc1_2e7_directIP_titration_rep1_Slot2-4_1_3515 20mio-rep2 E_20221201_NO30_400nL_HLAc1_2e7_directIP_titration_rep3_Slot2-4_1_3518.18333.1342.2 2 1 1 12.09 5.26 8.49 6.83 74.0 7 0 1 4660.0 Hydrogen 11 (M) 11 SSTKPGQLQLW (V) 11 QCIQRLQLW S S/T/K|P G Q|L|Q|L\W 54.5 6 5 6 4 0 dual b/y bias 63.323742 0.912853 622.831 1244.663 -0.0086 -6.9 -0.6000000000000005 8.47 3865.6 nuORFdb_human ENST00000488086.1_1_2:74432953-74433055:+ ENST00000488086.1_1_2:74432953-74433055:+|ENST00000394053.6_1_2:74432953-74434854:+ MTHFD2 ncRNA Processed Transcript lncRNA GN=MTHFD2 ncRNA Processed Transcript E_20221201_NO30_400nL_HLAc1_2e7_directIP_titration_rep3_Slot2-4_1_3518 40mio-rep1 E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep1_Slot1-04_1_3496.18459.1303.2 2 2 1 15.21 7.03 8.84 8.19 88.1 6 0 1 17400.0 Hydrogen 11 (M) 11 SSTKPGQLQLW (V) 11 SSPLFPGGLALW S S/T K|P G Q|L\Q|L\W 36.4 4 5 4 3 0 unbiased 63.763794 0.936938 622.8334 1244.663 -0.0046 -3.7 -0.5 8.47 3865.6 nuORFdb_human ENST00000488086.1_1_2:74432953-74433055:+ ENST00000488086.1_1_2:74432953-74433055:+|ENST00000394053.6_1_2:74432953-74434854:+ MTHFD2 ncRNA Processed Transcript lncRNA GN=MTHFD2 ncRNA Processed Transcript E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep1_Slot1-04_1_3496 40mio-rep2 E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep2_Slot2-5_1_3520.18214.1316.2 2 2 1 13.29 6.21 6.69 7.09 80.1 6 0 1 12700.0 Hydrogen 11 (M) 11 SSTKPGQLQLW (V) 11 IASVLmAPAGLW S S/T K|P G Q\L|Q|L\W 36.4 4 5 4 3 0 unbiased 62.918755 0.928824 622.8325 1244.663 -0.0046 -3.7 1.2000000000000002 8.47 3865.6 nuORFdb_human ENST00000488086.1_1_2:74432953-74433055:+ ENST00000488086.1_1_2:74432953-74433055:+|ENST00000394053.6_1_2:74432953-74434854:+ MTHFD2 ncRNA Processed Transcript lncRNA GN=MTHFD2 ncRNA Processed Transcript E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep2_Slot2-5_1_3520 40mio-rep3 E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep3_Slot2-5_1_3521.18249.1315.2 2 3 1 9.68 3.41 6.27 6.27 71.6 6 0 1 17200.0 Hydrogen 11 (M) 11 SSTKPGQLQLW (V) 11 AARLWGGSQLW S S/T K|P G Q\L|Q|L\W 36.4 4 5 4 3 0 unbiased 63.040607 0.929228 622.8331 1244.663 -0.0046 -3.7 0.8999999999999995 8.47 3865.6 nuORFdb_human ENST00000488086.1_1_2:74432953-74433055:+ ENST00000488086.1_1_2:74432953-74433055:+|ENST00000394053.6_1_2:74432953-74434854:+ MTHFD2 ncRNA Processed Transcript lncRNA GN=MTHFD2 ncRNA Processed Transcript E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep3_Slot2-5_1_3521 40mio-rep4 E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep4_Slot2-5_1_3522.18226.1326.2 2 2 1 12.21 3.05 8.34 9.16 71.5 7 0 1 13600.0 Hydrogen 11 (M) 11 SSTKPGQLQLW (V) 11 QCIQRLQLW S S/T K|P G\Q\L|Q|L\W 45.5 5 6 4 3 0 unbiased 62.943523 0.922684 622.8313 1244.663 -0.0086 -6.9 -2.2 8.47 3865.6 nuORFdb_human ENST00000488086.1_1_2:74432953-74433055:+ ENST00000488086.1_1_2:74432953-74433055:+|ENST00000394053.6_1_2:74432953-74434854:+ MTHFD2 ncRNA Processed Transcript lncRNA GN=MTHFD2 ncRNA Processed Transcript E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep4_Slot2-5_1_3522 40mio-rep1 E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep1_Slot1-04_1_3496.18073.1530.2 2 1 1 7.67 6.66 3.16 1.01 85.1 5 0 1 6940.0 Hydrogen 178 (K) 9 SSVPGLVLR (R) 9 SSVPGVRLL S S/V/P/G/L\V L R 44.4 4 1 4 0 0 y-ion bias 62.430588 0.794648 464.2809 927.562 -0.0075 -8.1 -4.8999999999999995 9.47 20473.6 nuORFdb_human ENST00000475841.2_1_12:132603503-132606470:+ ENST00000475841.2_1_12:132603503-132606470:+|ENST00000446190.5_1_12:132599077-132606470:+ EP400NL ncRNA Processed Transcript lncRNA GN=EP400NL ncRNA Processed Transcript E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep1_Slot1-04_1_3496 20mio-rep1 E_20221201_NO30_400nL_HLAc1_2e7_directIP_titration_rep1_Slot2-4_1_3515.11392.1515.2 2 2 1 10.83 6.23 6.07 4.6 74.0 6 1 1 10500.0 Hydrogen 106 (E) 9 SSWRAGQGW (Q) 9 SSRWGGAAGW S S/W/R A\G|Q|G\W 55.6 5 4 4 2 0 unbiased 39.347157 0.803867 517.7411 1034.48 -0.0055 -5.3 0.9000000000000004 9.47 17729.6 nuORFdb_human ENST00000357304.8_1_9:134308100-134321860:+ ENST00000357304.8_1_9:134308100-134321860:+ PRRC2B Out-of-Frame Out-of-Frame GN=PRRC2B Out-of-Frame E_20221201_NO30_400nL_HLAc1_2e7_directIP_titration_rep1_Slot2-4_1_3515 20mio-rep3 E_20221201_NO30_400nL_HLAc1_2e7_directIP_titration_rep4_Slot2-4_1_3519.11419.1509.2 2 1 1 9.12 7.27 3.14 1.85 71.5 6 1 1 6880.0 Hydrogen 106 (E) 9 SSWRAGQGW (Q) 9 SSGEQAGRGW S S/W/R A\G\Q|G\W 55.6 5 4 3 1 0 unbiased 39.447891 0.807811 517.7407 1034.48 -0.0055 -5.3 0.5 9.47 17729.6 nuORFdb_human ENST00000357304.8_1_9:134308100-134321860:+ ENST00000357304.8_1_9:134308100-134321860:+ PRRC2B Out-of-Frame Out-of-Frame GN=PRRC2B Out-of-Frame E_20221201_NO30_400nL_HLAc1_2e7_directIP_titration_rep4_Slot2-4_1_3519 40mio-rep1 E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep1_Slot1-04_1_3496.11675.1486.2 2 1 1 14.13 9.58 4.1 4.55 86.9 8 1 1 17500.0 Hydrogen 106 (E) 9 SSWRAGQGW (Q) 9 SSRWGGAAGW S/S/W/R-A|G\Q|G\W 100.0 9 4 5 2 1 unbiased 40.330181 0.822212 517.7414 1034.48 -0.0055 -5.3 -2.0999999999999996 9.47 17729.6 nuORFdb_human ENST00000357304.8_1_9:134308100-134321860:+ ENST00000357304.8_1_9:134308100-134321860:+ PRRC2B Out-of-Frame Out-of-Frame GN=PRRC2B Out-of-Frame E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep1_Slot1-04_1_3496 40mio-rep2 E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep2_Slot2-5_1_3520.11361.1498.2 2 1 1 12.77 9.42 4.93 3.35 84.8 6 1 1 8030.0 Hydrogen 106 (E) 9 SSWRAGQGW (Q) 9 SSRWGGAAGW S S/W/R A\G\Q|G\W 55.6 5 4 3 1 0 unbiased 39.246387 0.815012 517.7414 1034.48 -0.0055 -5.3 -0.39999999999999947 9.47 17729.6 nuORFdb_human ENST00000357304.8_1_9:134308100-134321860:+ ENST00000357304.8_1_9:134308100-134321860:+ PRRC2B Out-of-Frame Out-of-Frame GN=PRRC2B Out-of-Frame E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep2_Slot2-5_1_3520 40mio-rep3 E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep3_Slot2-5_1_3521.11379.1501.2 2 1 1 11.85 6.86 5.3 5.0 79.9 7 1 1 11400.0 Hydrogen 106 (E) 9 SSWRAGQGW (Q) 9 SSRWGGAAGW S/S/W/R A|G|Q|G\W 77.8 7 4 6 3 0 dual b/y bias 39.311718 0.812737 517.7412 1034.48 -0.0055 -5.3 -0.7000000000000002 9.47 17729.6 nuORFdb_human ENST00000357304.8_1_9:134308100-134321860:+ ENST00000357304.8_1_9:134308100-134321860:+ PRRC2B Out-of-Frame Out-of-Frame GN=PRRC2B Out-of-Frame E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep3_Slot2-5_1_3521 40mio-rep4 E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep4_Slot2-5_1_3522.11389.1500.2 2 1 1 15.17 10.07 6.96 5.1 88.2 7 1 1 8600.0 Hydrogen 106 (E) 9 SSWRAGQGW (Q) 9 SSRWGGAAGW S/S/W/R A|G\Q|G\W 77.8 7 4 5 2 0 unbiased 39.331367 0.813688 517.7414 1034.48 -0.0055 -5.3 -0.5999999999999996 9.47 17729.6 nuORFdb_human ENST00000357304.8_1_9:134308100-134321860:+ ENST00000357304.8_1_9:134308100-134321860:+ PRRC2B Out-of-Frame Out-of-Frame GN=PRRC2B Out-of-Frame E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep4_Slot2-5_1_3522 40mio-rep2 E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep2_Slot2-5_1_3520.9746.1451.2 2 1 1 10.71 6.94 2.67 3.77 65.8 8 1 1 8570.0 Hydrogen 11 (S) 9 SSWRGSQSW (T) 9 SSQCVRASW S/S/W/R/G\S\Q|S\W 100.0 9 4 5 1 0 unbiased 33.668472 0.84454 540.743 1080.486 -0.007 -6.5 -1.5999999999999996 9.47 10604.8 nuORFdb_human ENST00000636941.1_2_7:143344455-143344737:- ENST00000636941.1_2_7:143344455-143344737:- RP11-298A10.1 Out-of-Frame Out-of-Frame GN=RP11-298A10.1 Out-of-Frame E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep2_Slot2-5_1_3520 40mio-rep1 E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep1_Slot1-04_1_3496.6183.1449.2 2 1 1 11.28 6.12 6.59 5.16 72.1 7 2 2 7700.0 Hydrogen 57 (I) 9 STKNTKISW (A) 9 STTRIITSW S/T/K/N/T K\I|S\W 77.8 7 3 5 1 0 unbiased 21.367754 0.845597 532.7868 1064.573 -0.0068 -6.4 -3.2 10.0 14619.7 nuORFdb_human ENST00000263960.6_1_2:198339073-198339454:+ ENST00000263960.6_1_2:198339073-198339454:+|T061453_11:65696521-65697096:+|T184677_2:11569933-11570278:-|ENST00000467003.1_1_19:53888711-53888882:+ COQ10B 3' dORF 3' dORF GN=COQ10B 3' dORF E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep1_Slot1-04_1_3496 40mio-rep2 E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep2_Slot2-5_1_3520.5943.1458.2 2 1 1 9.55 4.42 6.69 5.13 68.7 6 2 2 11800.0 Hydrogen 57 (I) 9 STKNTKISW (A) 9 STTRIITSW S/T/K|N T K|I|S\W 66.7 6 4 5 3 0 dual b/y bias 20.537756 0.839917 532.7867 1064.573 -0.0068 -6.4 -1.5 10.0 14619.7 nuORFdb_human ENST00000263960.6_1_2:198339073-198339454:+ ENST00000263960.6_1_2:198339073-198339454:+|T061453_11:65696521-65697096:+|T184677_2:11569933-11570278:-|ENST00000467003.1_1_19:53888711-53888882:+ COQ10B 3' dORF 3' dORF GN=COQ10B 3' dORF E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep2_Slot2-5_1_3520 40mio-rep4 E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep4_Slot2-5_1_3522.5971.1458.2 2 1 1 9.15 4.91 5.65 4.24 68.9 7 2 2 5670.0 Hydrogen 57 (I) 9 STKNTKISW (A) 9 STTRIITSW S/T/K/N/T K\I|S\W 77.8 7 3 5 1 0 unbiased 20.627453 0.839874 532.7858 1064.573 -0.0088 -8.2 -3.499999999999999 10.0 14619.7 nuORFdb_human ENST00000263960.6_1_2:198339073-198339454:+ ENST00000263960.6_1_2:198339073-198339454:+|T061453_11:65696521-65697096:+|T184677_2:11569933-11570278:-|ENST00000467003.1_1_19:53888711-53888882:+ COQ10B 3' dORF 3' dORF GN=COQ10B 3' dORF E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep4_Slot2-5_1_3522 20mio-rep1 E_20221201_NO30_400nL_HLAc1_2e7_directIP_titration_rep1_Slot2-4_1_3515.18327.1390.2 2 1 1 8.79 6.52 3.92 2.27 60.4 5 0 1 4930.0 Hydrogen 12 (S) 10 STKPGQLQLW (V) 10 GALnELLQLW S T/K P G Q\L\Q|L\W 40.0 4 4 2 1 0 b-ion bias 63.29356 0.882759 579.3146 1157.631 -0.0086 -7.4 -1.2000000000000002 8.47 3865.6 nuORFdb_human ENST00000488086.1_1_2:74432953-74433055:+ ENST00000488086.1_1_2:74432953-74433055:+|ENST00000394053.6_1_2:74432953-74434854:+ MTHFD2 ncRNA Processed Transcript lncRNA GN=MTHFD2 ncRNA Processed Transcript E_20221201_NO30_400nL_HLAc1_2e7_directIP_titration_rep1_Slot2-4_1_3515 20mio-rep2 E_20221201_NO30_400nL_HLAc1_2e7_directIP_titration_rep3_Slot2-4_1_3518.18413.1386.2 2 1 1 9.3 5.27 4.25 4.03 66.8 6 0 1 6040.0 Hydrogen 12 (S) 10 STKPGQLQLW (V) 10 GNLNVTLQIW S T/K\P G Q\L\Q|L\W 50.0 5 5 2 1 0 b-ion bias 63.599876 0.884922 579.3152 1157.631 -0.0086 -7.4 -1.1000000000000005 8.47 3865.6 nuORFdb_human ENST00000488086.1_1_2:74432953-74433055:+ ENST00000488086.1_1_2:74432953-74433055:+|ENST00000394053.6_1_2:74432953-74434854:+ MTHFD2 ncRNA Processed Transcript lncRNA GN=MTHFD2 ncRNA Processed Transcript E_20221201_NO30_400nL_HLAc1_2e7_directIP_titration_rep3_Slot2-4_1_3518 40mio-rep2 E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep2_Slot2-5_1_3520.18294.1372.2 2 1 1 8.06 3.98 3.65 4.08 62.2 6 0 1 6160.0 Hydrogen 12 (S) 10 STKPGQLQLW (V) 10 KDLQNGIALW S T/K\P G Q\L\Q\L\W 50.0 5 5 1 0 0 b-ion bias 63.197739 0.893819 579.3166 1157.631 -0.0046 -4.0 0.9000000000000004 8.47 3865.6 nuORFdb_human ENST00000488086.1_1_2:74432953-74433055:+ ENST00000488086.1_1_2:74432953-74433055:+|ENST00000394053.6_1_2:74432953-74434854:+ MTHFD2 ncRNA Processed Transcript lncRNA GN=MTHFD2 ncRNA Processed Transcript E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep2_Slot2-5_1_3520 20mio-rep1 E_20221201_NO30_400nL_HLAc1_2e7_directIP_titration_rep1_Slot2-4_1_3515.12602.1548.2 2 2 1 12.66 9.04 4.63 3.62 84.6 7 1 1 6290.0 Hydrogen 37 (F) 9 STLPTVTKV (S) 9 STLPKSLSL S/T/L|P|T V|T\K-V 77.8 7 4 5 3 1 dual b/y bias 43.527138 0.783141 473.2801 945.562 -0.0088 -9.3 -3.1000000000000005 8.47 10189.7 nuORFdb_human ENST00000571129.5_1_17:7144048-7144743:- ENST00000571129.5_1_17:7144048-7144743:- GABARAP 3' dORF 3' dORF GN=GABARAP 3' dORF E_20221201_NO30_400nL_HLAc1_2e7_directIP_titration_rep1_Slot2-4_1_3515 20mio-rep3 E_20221201_NO30_400nL_HLAc1_2e7_directIP_titration_rep4_Slot2-4_1_3519.12646.1546.2 2 1 1 12.72 8.55 3.64 4.17 88.1 7 1 1 3240.0 Hydrogen 37 (F) 9 STLPTVTKV (S) 9 STLPKSLSL S/T/L|P/T V/T\K\V 77.8 7 3 5 1 0 unbiased 43.683956 0.784522 473.2799 945.562 -0.0088 -9.3 -3.500000000000001 8.47 10189.7 nuORFdb_human ENST00000571129.5_1_17:7144048-7144743:- ENST00000571129.5_1_17:7144048-7144743:- GABARAP 3' dORF 3' dORF GN=GABARAP 3' dORF E_20221201_NO30_400nL_HLAc1_2e7_directIP_titration_rep4_Slot2-4_1_3519 40mio-rep1 E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep1_Slot1-04_1_3496.12863.1519.2 2 2 1 15.79 9.6 6.19 6.19 91.0 8 1 1 13100.0 Hydrogen 37 (F) 9 STLPTVTKV (S) 9 STLPKSLSL S/T/L|P|T/V|T\K\V 100.0 9 5 6 3 0 dual b/y bias 44.432957 0.801807 473.2827 945.562 -0.0028 -3.0 0.20000000000000018 8.47 10189.7 nuORFdb_human ENST00000571129.5_1_17:7144048-7144743:- ENST00000571129.5_1_17:7144048-7144743:- GABARAP 3' dORF 3' dORF GN=GABARAP 3' dORF E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep1_Slot1-04_1_3496 40mio-rep2 E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep2_Slot2-5_1_3520.12588.1534.2 2 2 1 10.67 8.05 2.59 2.63 86.1 7 1 1 15500.0 Hydrogen 37 (F) 9 STLPTVTKV (S) 9 STLPKSLSL S T/L/P/T/V/T\K\V 77.8 7 2 5 0 0 y-ion bias 43.485416 0.792349 473.2816 945.562 -0.0048 -5.1 -0.1999999999999993 8.47 10189.7 nuORFdb_human ENST00000571129.5_1_17:7144048-7144743:- ENST00000571129.5_1_17:7144048-7144743:- GABARAP 3' dORF 3' dORF GN=GABARAP 3' dORF E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep2_Slot2-5_1_3520 40mio-rep3 E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep3_Slot2-5_1_3521.12589.1537.2 2 3 1 13.66 8.48 4.66 5.18 86.0 6 1 1 17300.0 Hydrogen 37 (F) 9 STLPTVTKV (S) 9 STLPKSLSL S T/L|P|T V|T\K\V 55.6 5 5 4 3 0 dual b/y bias 43.493061 0.790297 473.2825 945.562 -0.0048 -5.1 -0.5 8.47 10189.7 nuORFdb_human ENST00000571129.5_1_17:7144048-7144743:- ENST00000571129.5_1_17:7144048-7144743:- GABARAP 3' dORF 3' dORF GN=GABARAP 3' dORF E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep3_Slot2-5_1_3521 40mio-rep4 E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep4_Slot2-5_1_3522.12589.1535.2 2 1 1 10.02 6.85 3.12 3.17 78.9 6 1 1 7040.0 Hydrogen 37 (F) 9 STLPTVTKV (S) 9 STLPKSLSL S T/L|P/T V/T\K\V 55.6 5 3 4 1 0 unbiased 43.47419 0.791349 473.2823 945.562 -0.0048 -5.1 -0.39999999999999947 8.47 10189.7 nuORFdb_human ENST00000571129.5_1_17:7144048-7144743:- ENST00000571129.5_1_17:7144048-7144743:- GABARAP 3' dORF 3' dORF GN=GABARAP 3' dORF E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep4_Slot2-5_1_3522 1mio-rep1 E_20221201_NO30_400nL_HLAc1_1e6_directIP_titration_rep1_Slot1-06_1_3498.16236.1482.2 2 1 1 12.81 5.2 6.24 7.61 85.0 6 1 1 4130.0 Hydrogen 94 (N) 9 STSTILRSF (S) 9 ASHILISVDG S T/S/T|I/L|R\S-F 77.8 7 3 5 2 1 unbiased 56.076694 0.824753 506.2752 1011.547 -0.0042 -4.2 0.7000000000000002 9.47 12004.5 nuORFdb_human ENST00000559798.2_2_15:69488756-69489068:+ ENST00000559798.2_2_15:69488756-69489068:+ GLCE ncRNA Processed Transcript lncRNA GN=GLCE ncRNA Processed Transcript E_20221201_NO30_400nL_HLAc1_1e6_directIP_titration_rep1_Slot1-06_1_3498 10mio-rep2 E_20221201_NO30_400nL_HLAc1_1e7_directIP_titration_rep2_Slot2-3_1_3513.15912.1521.2 2 4 1 13.32 6.04 7.6 7.29 84.4 7 1 1 43900.0 Hydrogen 94 (N) 9 STSTILRSF (S) 9 TSTSLKDLF S T/S/T/I/L|R|S\F 77.8 7 3 6 2 0 y-ion bias 54.965145 0.800061 506.2745 1011.547 -0.0062 -6.1 0.6000000000000005 9.47 12004.5 nuORFdb_human ENST00000559798.2_2_15:69488756-69489068:+ ENST00000559798.2_2_15:69488756-69489068:+ GLCE ncRNA Processed Transcript lncRNA GN=GLCE ncRNA Processed Transcript E_20221201_NO30_400nL_HLAc1_1e7_directIP_titration_rep2_Slot2-3_1_3513 10mio-rep3 E_20221201_NO30_400nL_HLAc1_1e7_directIP_titration_rep3_Slot2-3_1_3514.15868.1489.2 2 7 1 12.13 3.76 7.45 8.37 82.1 6 1 1 28800.0 Hydrogen 94 (N) 9 STSTILRSF (S) 9 ASHILISVDG S T/S/T|I/L|R|S-F 77.8 7 3 6 3 1 dual b/y bias 54.815899 0.820723 506.2733 1011.547 -0.0082 -8.1 -1.7999999999999998 9.47 12004.5 nuORFdb_human ENST00000559798.2_2_15:69488756-69489068:+ ENST00000559798.2_2_15:69488756-69489068:+ GLCE ncRNA Processed Transcript lncRNA GN=GLCE ncRNA Processed Transcript E_20221201_NO30_400nL_HLAc1_1e7_directIP_titration_rep3_Slot2-3_1_3514 20mio-rep1 E_20221201_NO30_400nL_HLAc1_2e7_directIP_titration_rep1_Slot2-4_1_3515.15853.1489.2 2 4 1 13.31 6.83 7.05 6.48 85.7 7 1 1 66200.0 Hydrogen 94 (N) 9 STSTILRSF (S) 9 TSTSLKDLF S T/S/T/I/L|R|S\F 77.8 7 3 6 2 0 y-ion bias 54.750961 0.820233 506.2738 1011.547 -0.0062 -6.1 0.10000000000000053 9.47 12004.5 nuORFdb_human ENST00000559798.2_2_15:69488756-69489068:+ ENST00000559798.2_2_15:69488756-69489068:+ GLCE ncRNA Processed Transcript lncRNA GN=GLCE ncRNA Processed Transcript E_20221201_NO30_400nL_HLAc1_2e7_directIP_titration_rep1_Slot2-4_1_3515 20mio-rep2 E_20221201_NO30_400nL_HLAc1_2e7_directIP_titration_rep3_Slot2-4_1_3518.15952.1486.2 2 6 1 12.0 4.77 8.19 7.23 82.0 7 1 1 74000.0 Hydrogen 94 (N) 9 STSTILRSF (S) 9 TSTSLKDLF S T/S/T|I/L|R|S\F 77.8 7 4 6 3 0 dual b/y bias 55.098164 0.822113 506.2731 1011.547 -0.0082 -8.1 -1.7999999999999998 9.47 12004.5 nuORFdb_human ENST00000559798.2_2_15:69488756-69489068:+ ENST00000559798.2_2_15:69488756-69489068:+ GLCE ncRNA Processed Transcript lncRNA GN=GLCE ncRNA Processed Transcript E_20221201_NO30_400nL_HLAc1_2e7_directIP_titration_rep3_Slot2-4_1_3518 20mio-rep3 E_20221201_NO30_400nL_HLAc1_2e7_directIP_titration_rep4_Slot2-4_1_3519.15941.1486.2 2 5 1 14.26 5.68 8.98 8.58 85.4 7 1 1 62100.0 Hydrogen 94 (N) 9 STSTILRSF (S) 9 TSTSLKDLF S T/S/T|I/L|R|S\F 77.8 7 4 6 3 0 dual b/y bias 55.060776 0.822534 506.2744 1011.547 -0.0062 -6.1 -0.2999999999999998 9.47 12004.5 nuORFdb_human ENST00000559798.2_2_15:69488756-69489068:+ ENST00000559798.2_2_15:69488756-69489068:+ GLCE ncRNA Processed Transcript lncRNA GN=GLCE ncRNA Processed Transcript E_20221201_NO30_400nL_HLAc1_2e7_directIP_titration_rep4_Slot2-4_1_3519 40mio-rep1 E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep1_Slot1-04_1_3496.16117.1463.2 2 5 1 15.83 5.52 10.74 10.31 86.2 7 1 1 204000.0 Hydrogen 94 (N) 9 STSTILRSF (S) 9 TSTSLKDLF S T/S/T|I/L|R|S\F 77.8 7 4 6 3 0 dual b/y bias 55.672729 0.836845 506.2758 1011.547 -0.0022 -2.2 1.0 9.47 12004.5 nuORFdb_human ENST00000559798.2_2_15:69488756-69489068:+ ENST00000559798.2_2_15:69488756-69489068:+ GLCE ncRNA Processed Transcript lncRNA GN=GLCE ncRNA Processed Transcript E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep1_Slot1-04_1_3496 40mio-rep2 E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep2_Slot2-5_1_3520.15831.1474.2 2 4 1 15.92 6.3 9.97 9.61 88.0 7 1 1 221000.0 Hydrogen 94 (N) 9 STSTILRSF (S) 9 STSRLTLFS S T/S/T|I/L|R|S\F 77.8 7 4 6 3 0 dual b/y bias 54.686321 0.83 506.2761 1011.547 -0.0022 -2.2 2.7 9.47 12004.5 nuORFdb_human ENST00000559798.2_2_15:69488756-69489068:+ ENST00000559798.2_2_15:69488756-69489068:+ GLCE ncRNA Processed Transcript lncRNA GN=GLCE ncRNA Processed Transcript E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep2_Slot2-5_1_3520 40mio-rep3 E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep3_Slot2-5_1_3521.15842.1479.2 2 3 1 15.38 5.4 10.18 9.98 88.5 8 1 1 125000.0 Hydrogen 94 (N) 9 STSTILRSF (S) 9 STSRLTLFS S/T/S/T|I/L|R|S\F 100.0 9 4 7 3 0 dual b/y bias 54.725529 0.826785 506.2762 1011.547 -0.0022 -2.2 2.3999999999999995 9.47 12004.5 nuORFdb_human ENST00000559798.2_2_15:69488756-69489068:+ ENST00000559798.2_2_15:69488756-69489068:+ GLCE ncRNA Processed Transcript lncRNA GN=GLCE ncRNA Processed Transcript E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep3_Slot2-5_1_3521 40mio-rep4 E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep4_Slot2-5_1_3522.15809.1476.2 2 4 1 14.78 3.65 9.12 11.13 86.6 8 1 1 178000.0 Hydrogen 94 (N) 9 STSTILRSF (S) 9 ASHILISVDG S/T/S/T|I/L|R\S\F 100.0 9 4 6 2 0 unbiased 54.596817 0.828779 506.2751 1011.547 -0.0042 -4.2 0.5 9.47 12004.5 nuORFdb_human ENST00000559798.2_2_15:69488756-69489068:+ ENST00000559798.2_2_15:69488756-69489068:+ GLCE ncRNA Processed Transcript lncRNA GN=GLCE ncRNA Processed Transcript E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep4_Slot2-5_1_3522 5mio-rep1 E_20221201_NO30_400nL_HLAc1_5e6_directIP_titration_rep1_Slot1-37_1_3502.16207.1486.2 2 3 1 12.14 6.51 6.63 5.64 81.4 7 1 1 13800.0 Hydrogen 94 (N) 9 STSTILRSF (S) 9 TSTSLKDLF S T/S/T/I/L|R\S\F 77.8 7 3 5 1 0 unbiased 55.983582 0.82208 506.2723 1011.547 -0.0102 -10.1 -3.0999999999999996 9.47 12004.5 nuORFdb_human ENST00000559798.2_2_15:69488756-69489068:+ ENST00000559798.2_2_15:69488756-69489068:+ GLCE ncRNA Processed Transcript lncRNA GN=GLCE ncRNA Processed Transcript E_20221201_NO30_400nL_HLAc1_5e6_directIP_titration_rep1_Slot1-37_1_3502 5mio-rep2 E_20221201_NO30_400nL_HLAc1_5e6_directIP_titration_rep2_Slot2-2_1_3510.16071.1490.2 2 2 1 12.3 5.82 8.3 6.48 78.4 7 1 1 7260.0 Hydrogen 94 (N) 9 STSTILRSF (S) 9 TSTSLKDLF S T/S/T|I/L/R|S\F 77.8 7 3 6 2 0 y-ion bias 55.509148 0.819927 506.2713 1011.547 -0.0122 -12.1 -3.4000000000000004 9.47 12004.5 nuORFdb_human ENST00000559798.2_2_15:69488756-69489068:+ ENST00000559798.2_2_15:69488756-69489068:+ GLCE ncRNA Processed Transcript lncRNA GN=GLCE ncRNA Processed Transcript E_20221201_NO30_400nL_HLAc1_5e6_directIP_titration_rep2_Slot2-2_1_3510 5mio-rep3 E_20221201_NO30_400nL_HLAc1_5e6_directIP_titration_rep3_Slot2-2_1_3511.16081.1486.2 2 1 1 9.39 3.34 4.79 6.05 72.2 6 1 1 4120.0 Hydrogen 94 (N) 9 STSTILRSF (S) 9 PSELLLPDQ S T/S/T/I/L|R|S-F 77.8 7 2 6 2 1 y-ion bias 55.545395 0.822171 506.2743 1011.547 -0.0062 -6.1 2.0999999999999996 9.47 12004.5 nuORFdb_human ENST00000559798.2_2_15:69488756-69489068:+ ENST00000559798.2_2_15:69488756-69489068:+ GLCE ncRNA Processed Transcript lncRNA GN=GLCE ncRNA Processed Transcript E_20221201_NO30_400nL_HLAc1_5e6_directIP_titration_rep3_Slot2-2_1_3511 40mio-rep1 E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep1_Slot1-04_1_3496.13293.500.1 1 1 1 8.01 3.0 5.14 5.01 70.6 6 0 0 11300.0 Hydrogen 24 (C) 9 STTPTTATW (R) 9 TSTLSSSPW S T/T/P T/T\A\T\W 55.6 5 3 3 0 0 unbiased 45.918465 1.426099 965.4556 965.457 -0.0015 -1.5 1.7000000000000002 5.24 6063.9 nuORFdb_human ENST00000374492.7_1_20:33732813-33735003:- ENST00000374492.7_1_20:33732813-33735003:- EDEM2 Out-of-Frame Out-of-Frame GN=EDEM2 Out-of-Frame E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep1_Slot1-04_1_3496 40mio-rep2 E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep2_Slot2-5_1_3520.12986.514.1 1 1 1 8.23 3.28 6.0 4.95 64.9 6 0 0 4100.0 Hydrogen 24 (C) 9 STTPTTATW (R) 9 TSTLSSSPW S T/T/P T/T\A\T\W 55.6 5 3 3 0 0 unbiased 44.859909 1.417533 965.4528 965.457 -0.0045 -4.6 0.3000000000000007 5.24 6063.9 nuORFdb_human ENST00000374492.7_1_20:33732813-33735003:- ENST00000374492.7_1_20:33732813-33735003:- EDEM2 Out-of-Frame Out-of-Frame GN=EDEM2 Out-of-Frame E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep2_Slot2-5_1_3520 40mio-rep3 E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep3_Slot2-5_1_3521.12985.515.1 1 1 1 7.75 1.94 4.57 5.82 60.0 6 0 0 4710.0 Hydrogen 24 (C) 9 STTPTTATW (R) 9 AEARMTTW S T/T/P T|T\A\T\W 55.6 5 4 3 1 0 unbiased 44.860779 1.417059 965.4525 965.457 -0.0045 -4.6 0.0 5.24 6063.9 nuORFdb_human ENST00000374492.7_1_20:33732813-33735003:- ENST00000374492.7_1_20:33732813-33735003:- EDEM2 Out-of-Frame Out-of-Frame GN=EDEM2 Out-of-Frame E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep3_Slot2-5_1_3521 40mio-rep4 E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep4_Slot2-5_1_3522.12978.514.1 1 1 1 7.57 2.6 5.43 4.97 65.1 6 0 0 3320.0 Hydrogen 24 (C) 9 STTPTTATW (R) 9 TSTLSSSPW S T/T/P T/T\A\T\W 55.6 5 3 3 0 0 unbiased 44.817051 1.417797 965.4537 965.457 -0.0035 -3.6 1.1 5.24 6063.9 nuORFdb_human ENST00000374492.7_1_20:33732813-33735003:- ENST00000374492.7_1_20:33732813-33735003:- EDEM2 Out-of-Frame Out-of-Frame GN=EDEM2 Out-of-Frame E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep4_Slot2-5_1_3522 40mio-rep3 E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep3_Slot2-5_1_3521.10022.1507.2 2 1 1 7.0 5.2 2.43 1.8 52.7 5 1 1 7960.0 Hydrogen 84 (S) 8 SVIMMKLV (L) 8 LVSYSRPV S/V/I/M M K\L\V 62.5 5 2 3 0 0 unbiased 34.623508 0.809169 460.7668 920.531 -0.004 -4.4 0.1999999999999993 8.47 11119.5 nuORFdb_human ENST00000521804.1_1_8:142264568-142318349:- ENST00000521804.1_1_8:142264568-142318349:- SLC45A4 ncRNA Processed Transcript lncRNA GN=SLC45A4 ncRNA Processed Transcript E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep3_Slot2-5_1_3521 40mio-rep2 E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep2_Slot2-5_1_3520.21472.1452.2 2 1 1 9.02 5.77 4.53 3.24 76.6 7 0 0 6030.0 Hydrogen 23 (M) 9 SVLGEMTHW (L) 9 ESEEEVGIPA S V/L|G/E/M/T/H-W 77.8 7 1 6 1 1 y-ion bias 74.176956 0.84348 530.2473 1059.493 -0.0061 -5.7 -0.7999999999999998 5.22 4172.8 nuORFdb_human ENST00000557673.5_3_14:75590889-75593687:- ENST00000557673.5_3_14:75590889-75593687:- NEK9 5' uORF 5' uORF GN=NEK9 5' uORF E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep2_Slot2-5_1_3520 SCP E_20221201_NO30_400nL_HLAc1_4e7_PDAC_Slot2-6_1_3524.30123.348.1 1 1 1 9.05 5.91 4.24 3.14 73.1 6 0 0 24700.0 Hydrogen 20 (F) 9 SVMSLVIFV (Q) 9 VIGPYSIFV S V M\S\L\V\I\F\V 66.7 6 6 0 0 0 b-ion bias 104.073219 1.51608 994.5638 994.564 -0.0002 -0.2 2.1999999999999997 5.24 9781.7 nuORFdb_human ENST00000361804.4_1_10:112328768-112337656:+ ENST00000361804.4_1_10:112328768-112337656:+ SMC3 Out-of-Frame Out-of-Frame GN=SMC3 Out-of-Frame E_20221201_NO30_400nL_HLAc1_4e7_PDAC_Slot2-6_1_3524 Eva-50mio-3fr AL20210827_EV_HLA_PDAC_PANFRO413_2D_50e6_W632_classI_fxn3.52181.52181.2 2 1 1 9.16 1.72 6.27 7.44 77.5 6 0 0 3890000.0 M22m Hydrogen 20 (F) 9 SVmSLVIFV (Q) 9 LVADFSIVF S V m\S\L\V\I/F/V 66.7 6 4 2 0 0 b-ion bias m:Oxidized methionine 0.95 100.0 93.98 100.0 0.0 505.7849 994.564 15.9984 3.5 3.0 5.24 9781.7 nuORFdb_human ENST00000361804.4_1_10:112328768-112337656:+ ENST00000361804.4_1_10:112328768-112337656:+ SMC3 Out-of-Frame Out-of-Frame GN=SMC3 Out-of-Frame AL20210827_EV_HLA_PDAC_PANFRO413_2D_50e6_W632_classI_fxn3 40mio-rep1 E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep1_Slot1-04_1_3496.9456.1466.2 2 2 1 11.13 6.46 4.67 4.67 76.3 7 1 2 19900.0 Hydrogen 32 (V) 8 SVNVRKTF (S) 8 VSLnLRTF S/V/N/V/R-K\T\F 100.0 8 2 4 0 1 y-ion bias 32.667934 0.835174 475.773 950.542 -0.0031 -3.2 0.0 11.0 5659.3 nuORFdb_human ENST00000396836.6_1_5:55147345-55155373:+ ENST00000396836.6_1_5:55147345-55155373:+ IL31RA 5' Overlap uORF 5' Overlap uORF GN=IL31RA 5' Overlap uORF E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep1_Slot1-04_1_3496 20mio-rep1 E_20221201_NO30_400nL_HLAc1_2e7_directIP_titration_rep1_Slot2-4_1_3515.13905.655.1 1 1 1 8.25 4.53 4.22 3.73 66.8 6 0 0 1670.0 Hydrogen 336 (H) 9 SVPEAGPSL (E) 9 APVSEGSPL S V/P/E/A/G|P S\L 55.6 5 2 5 1 0 y-ion bias 48.026241 1.333027 856.439 856.441 -0.0021 -2.4 3.8000000000000003 4.0 69407.9 nuORFdb_human ENST00000614000.4_1_17:35928891-35960656:- ENST00000614000.4_1_17:35928891-35960656:-|ENST00000619976.1_1_17:35932068-35969451:- SYNRG 5' Overlap uORF 5' Overlap uORF GN=SYNRG 5' Overlap uORF E_20221201_NO30_400nL_HLAc1_2e7_directIP_titration_rep1_Slot2-4_1_3515 10mio-rep2 E_20221201_NO30_400nL_HLAc1_1e7_directIP_titration_rep2_Slot2-3_1_3513.19217.1438.2 2 2 1 8.55 7.53 3.08 1.01 81.1 6 1 1 6220.0 Hydrogen 7 (C) 9 SVTRSSFLW (C) 9 VSEKGYTLW S V/T/R/S S\F|L\W 55.6 5 3 4 1 0 unbiased 66.378899 0.852388 541.779 1082.563 -0.0122 -11.3 -4.6000000000000005 9.47 4827.7 nuORFdb_human ENST00000372455.4_1_1:43852626-43853250:- ENST00000372455.4_1_1:43852626-43853250:- MED8 5' Overlap uORF 5' Overlap uORF GN=MED8 5' Overlap uORF E_20221201_NO30_400nL_HLAc1_1e7_directIP_titration_rep2_Slot2-3_1_3513 40mio-rep2 E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep2_Slot2-5_1_3520.28473.276.1 1 1 1 8.13 3.35 7.09 4.78 72.1 6 0 0 6620.0 Hydrogen 27 (N) 9 SVYSLTDLW (F) 9 GFLnTSFPSL S V Y\S\L\T\D\L\W 66.7 6 6 0 0 0 b-ion bias 98.361496 1.558311 1083.5296 1083.536 -0.0057 -5.3 -0.39999999999999947 3.8 5208.9 nuORFdb_human ENST00000334534.9_1_20:10579325-10582447:+ ENST00000334534.9_1_20:10579325-10582447:+ SLX4IP Out-of-Frame Out-of-Frame GN=SLX4IP Out-of-Frame E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep2_Slot2-5_1_3520 40mio-rep3 E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep3_Slot2-5_1_3521.28474.278.1 1 1 1 11.47 5.75 6.7 5.72 75.8 7 0 0 7580.0 Hydrogen 27 (N) 9 SVYSLTDLW (F) 9 GFLnTSFPSL S V/Y\S\L|T\D|L\W 77.8 7 6 3 2 0 b-ion bias 98.357697 1.557096 1083.5297 1083.536 -0.0057 -5.3 -0.7000000000000002 3.8 5208.9 nuORFdb_human ENST00000334534.9_1_20:10579325-10582447:+ ENST00000334534.9_1_20:10579325-10582447:+ SLX4IP Out-of-Frame Out-of-Frame GN=SLX4IP Out-of-Frame E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep3_Slot2-5_1_3521 40mio-rep4 E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep4_Slot2-5_1_3522.28473.276.1 1 1 1 12.0 3.25 11.16 8.74 76.8 7 0 0 5400.0 Hydrogen 27 (N) 9 SVYSLTDLW (F) 9 ESLYSSVInA S V/Y|S|L\T\D\L\W 77.8 7 6 3 2 0 b-ion bias 98.344482 1.558504 1083.5317 1083.536 -0.0057 -5.3 -0.5999999999999996 3.8 5208.9 nuORFdb_human ENST00000334534.9_1_20:10579325-10582447:+ ENST00000334534.9_1_20:10579325-10582447:+ SLX4IP Out-of-Frame Out-of-Frame GN=SLX4IP Out-of-Frame E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep4_Slot2-5_1_3522 SCP E_20221201_NO30_400nL_HLAc1_4e7_PDAC_Slot2-6_1_3524.14887.1367.2 2 2 1 16.21 12.59 4.67 3.62 83.9 6 0 0 71000.0 Hydrogen 180 (G) 9 SWLGQQLHL (S) 9 WEKQLQHI S W|L|G|Q|Q L|H\L 55.6 5 6 5 5 0 dual b/y bias 51.431599 0.896759 541.2911 1081.579 -0.0042 -3.9 -1.5 6.46 28244.7 nuORFdb_human ENST00000473097.5_1_1:1288733-1289815:- ENST00000473097.5_1_1:1288733-1289815:- MXRA8 3' Overlap dORF 3' Overlap dORF GN=MXRA8 3' Overlap dORF E_20221201_NO30_400nL_HLAc1_4e7_PDAC_Slot2-6_1_3524 SCP E_20221201_NO30_400nL_HLAc1_4e7_PDAC_Slot2-6_1_3524.28065.276.1 1 2 1 7.42 -0.53 7.42 7.95 53.2 7 0 0 20900.0 Hydrogen 31 (E) 9 SWSAVAHFF (L) 9 FSMLFLmF S W/S/A|V/A|H\F\F 77.8 7 4 5 2 0 unbiased 96.959915 1.558556 1051.4986 1051.5 0.0004 0.4 2.8 6.46 5078.5 nuORFdb_human ENST00000419892.6_1_6:46996750-47009968:- ENST00000419892.6_1_6:46996750-47009968:- ADGRF1 ncRNA Retained Intron lncRNA GN=ADGRF1 ncRNA Retained Intron E_20221201_NO30_400nL_HLAc1_4e7_PDAC_Slot2-6_1_3524 Eva-50mio-3fr AL20210827_EV_HLA_PDAC_PANFRO413_2D_50e6_W632_classI_fxn2.8699.8699.3 3 4 3 12.04 12.04 12.04 58.9 8 2 2 63400000.0 Hydrogen 3 (G) 9 SYAKSTQRF (-) 9 S/Y/A/K/S/T/Q/R-F 100.0 9 0 7 0 1 y-ion bias 1.0 62.7 17.48 55.82 0.0 363.1891 1087.553 -0.0004 -0.3 -0.6 9.99 1275.4 nuORFdb_human ENST00000430580.6_1_1:16939836-16939869:- ENST00000430580.6_1_1:16939836-16939869:- NBPF1 5' uORF 5' uORF GN=NBPF1 5' uORF AL20210827_EV_HLA_PDAC_PANFRO413_2D_50e6_W632_classI_fxn2 Exploris AL20221201_MKP_PDAC_01.9473.9582.2 2 2 1 12.79 3.98 8.14 8.81 86.8 6 2 2 204000000.0 Hydrogen 3 (G) 9 SYAKSTQRF (-) 9 SYmAVIIQF S/Y|A|K/S/T Q R-F 66.7 6 2 5 2 1 y-ion bias 1.0 100.0 18.12 100.0 0.0 544.2805 1087.553 0.0007 0.7 -0.10000000000000009 9.99 1275.4 nuORFdb_human ENST00000430580.6_1_1:16939836-16939869:- ENST00000430580.6_1_1:16939836-16939869:- NBPF1 5' uORF 5' uORF GN=NBPF1 5' uORF AL20221201_MKP_PDAC_01 SCP E_20221201_NO30_400nL_HLAc1_4e7_PDAC_Slot2-6_1_3524.3468.1371.2 2 3 1 11.71 4.26 7.09 7.45 69.7 8 2 2 110000.0 Hydrogen 3 (G) 9 SYAKSTQRF (-) 9 SLPGGPEARGF S/Y|A|K|S/T\Q/R\F 100.0 9 5 6 3 0 dual b/y bias 11.994093 0.894292 544.2785 1087.553 -0.0024 -2.2 0.19999999999999973 9.99 1275.4 nuORFdb_human ENST00000430580.6_1_1:16939836-16939869:- ENST00000430580.6_1_1:16939836-16939869:- NBPF1 5' uORF 5' uORF GN=NBPF1 5' uORF E_20221201_NO30_400nL_HLAc1_4e7_PDAC_Slot2-6_1_3524 Exploris_FAIMS VH20221207_MKP_FAIMS2022E_HLAc1_4e7_PDAC.13576.13671.3 3 1 1 10.32 3.32 6.22 7.0 80.7 6 2 2 25200000.0 Hydrogen 3 (G) 9 SYAKSTQRF (-) 9 HSPKEVMFL S Y/A/K/S/T/Q R-F 55.6 5 0 5 0 1 y-ion bias 0.999 92.6 29.48 22.87 -50.0 363.1885 1087.553 -0.0022 -2.0 0.6000000000000001 9.99 1275.4 nuORFdb_human ENST00000430580.6_1_1:16939836-16939869:- ENST00000430580.6_1_1:16939836-16939869:- NBPF1 5' uORF 5' uORF GN=NBPF1 5' uORF VH20221207_MKP_FAIMS2022E_HLAc1_4e7_PDAC Exploris_FAIMS VH20221207_MKP_FAIMS2022E_HLAc1_4e7_PDAC.29017.29017.2 2 1 1 8.35 5.63 2.73 2.73 67.0 5 1 1 1190000.0 Hydrogen 35 (W) 9 SYAVKASTF (L) 9 SYAIFGSKT S Y|A|V/K-A S T/F 44.4 4 2 4 2 1 y-ion bias 0.979 51.8 57.25 120.0 -70.0 487.2518 973.499 -0.0025 -2.6 0.0 8.31 5511.4 nuORFdb_human ENST00000624069.3_1_19:49990877-49993734:+ ENST00000624069.3_1_19:49990877-49993734:+ RPL13A Nonsense Mediated Decay Other GN=RPL13A Nonsense Mediated Decay VH20221207_MKP_FAIMS2022E_HLAc1_4e7_PDAC SCP E_20221201_NO30_400nL_HLAc1_4e7_PDAC_Slot2-6_1_3524.12689.387.1 1 1 1 7.28 2.45 3.23 4.82 62.4 6 0 0 3410.0 Hydrogen 3 (G) 9 SYGEVHPAF (L) 9 LHGYSDLDS S Y/G/E/V/H\P A\F 55.6 5 2 4 0 0 y-ion bias 43.835659 1.49286 1006.4609 1006.463 -0.0029 -2.9 -0.5 5.22 3082.3 smORF_v4|nuORFdb_human NBPF3_1:21766804-21771489:+ NBPF3_1:21766804-21771489:+|ENST00000454000.6_1_1:21766746-21771486:+|ENST00000475869.2_1_1:21766701-21766842:+ NBPF3 uORF 1:21766804-21771489:+ 1 NBPF3 5' uORF 5' uORF GN=NBPF3 E_20221201_NO30_400nL_HLAc1_4e7_PDAC_Slot2-6_1_3524 Eva-50mio-3fr AL20210827_EV_HLA_PDAC_PANFRO413_2D_50e6_W632_classI_fxn1.12609.12609.3 3 1 1 10.29 7.58 5.07 2.71 72.7 5 1 1 5920000.0 Hydrogen 398 (N) 9 SYLENHQRI (H) 9 SYLnQHQRI S Y|L/E/N/H/Q R-I 55.6 5 1 5 1 1 y-ion bias 0.995 91.3 24.67 100.0 0.0 387.2001 1159.585 0.0003 0.3 0.19999999999999998 6.47 63082.9 nuORFdb_human ENST00000598377.2_2_19:53707315-53715759:+ ENST00000598377.2_2_19:53707315-53715759:+ CTD-2245F17.3 Pseudogene Pseudogene GN=CTD-2245F17.3 Pseudogene AL20210827_EV_HLA_PDAC_PANFRO413_2D_50e6_W632_classI_fxn1 Exploris AL20221201_MKP_PDAC_01.15394.15394.3 3 1 1 11.18 7.81 7.14 3.37 72.7 6 1 1 41000000.0 Hydrogen 398 (N) 9 SYLENHQRI (H) 9 SYLnQHQRI S Y/L/E/N/H/Q R/I 55.6 5 0 6 0 0 y-ion bias 0.965 68.3 27.77 52.83 0.0 387.2014 1159.585 0.0042 3.6 2.8 6.47 63082.9 nuORFdb_human ENST00000598377.2_2_19:53707315-53715759:+ ENST00000598377.2_2_19:53707315-53715759:+ CTD-2245F17.3 Pseudogene Pseudogene GN=CTD-2245F17.3 Pseudogene AL20221201_MKP_PDAC_01 SCP E_20221201_NO30_400nL_HLAc1_4e7_PDAC_Slot2-6_1_3524.6837.1318.2 2 2 1 17.58 13.12 10.52 4.46 86.8 8 1 1 34300.0 Hydrogen 398 (N) 9 SYLENHQRI (H) 9 SYLnQHQRI S/Y|L|E/N\H|Q|R\I 100.0 9 6 6 4 0 dual b/y bias 23.624146 0.927743 580.2949 1159.585 -0.0027 -2.3 0.10000000000000009 6.47 63082.9 nuORFdb_human ENST00000598377.2_2_19:53707315-53715759:+ ENST00000598377.2_2_19:53707315-53715759:+ CTD-2245F17.3 Pseudogene Pseudogene GN=CTD-2245F17.3 Pseudogene E_20221201_NO30_400nL_HLAc1_4e7_PDAC_Slot2-6_1_3524 Exploris AL20221201_MKP_PDAC_01.63701.63701.2 2 1 1 8.96 5.14 2.21 3.82 50.1 5 1 1 846000000.0 Hydrogen 107 (A) 9 SYLRQLELF (L) 9 SRQELLYLF S Y/L R Q\L\E|L|F 44.4 4 4 3 2 0 unbiased 0.992 100.0 95.8 94.68 0.0 584.8192 1168.636 -0.005 -4.2 -5.0 5.72 15376.8 nuORFdb_human ENST00000344823.9_1_16:3451289-3451709:+ ENST00000344823.9_1_16:3451289-3451709:+ ZNF174 5' uORF 5' uORF GN=ZNF174 5' uORF AL20221201_MKP_PDAC_01 Eva-50mio-3fr AL20210827_EV_HLA_PDAC_PANFRO413_2D_50e6_W632_classI_fxn3.51943.51943.2 2 1 1 8.9 5.26 3.65 3.65 67.6 6 0 0 3060000.0 Hydrogen 5 (S) 9 SYPAFWTSF (I) 9 QYSFWDLF S Y\P A-F-W-T/S/F 55.6 5 1 2 0 3 internal-ion bias 0.937 45.7 93.37 100.0 0.0 553.2548 1105.499 0.0033 3.0 2.5 5.24 2753.1 nuORFdb_human ENST00000299505.6_1_19:34791118-34791184:+ ENST00000299505.6_1_19:34791118-34791184:+ KIAA0355 5' uORF 5' uORF GN=KIAA0355 5' uORF AL20210827_EV_HLA_PDAC_PANFRO413_2D_50e6_W632_classI_fxn3 SCP E_20221201_NO30_400nL_HLAc1_4e7_PDAC_Slot2-6_1_3524.28926.297.1 1 1 1 15.41 5.47 12.5 9.94 93.7 6 0 0 17600.0 Hydrogen 5 (S) 9 SYPAFWTSF (I) 9 SYPALCFTF S Y/P A|F|W\T\S\F 55.6 5 5 3 2 0 unbiased 99.935799 1.546035 1105.4965 1105.499 0.0011 1.0 3.4 5.24 2753.1 nuORFdb_human ENST00000299505.6_1_19:34791118-34791184:+ ENST00000299505.6_1_19:34791118-34791184:+ KIAA0355 5' uORF 5' uORF GN=KIAA0355 5' uORF E_20221201_NO30_400nL_HLAc1_4e7_PDAC_Slot2-6_1_3524 Eva-50mio-3fr AL20210827_EV_HLA_PDAC_PANFRO413_2D_50e6_W632_classI_fxn3.16641.16750.2 2 2 1 14.06 8.54 5.51 5.51 85.6 6 1 2 656000000.0 Hydrogen 20 (H) 9 SYPRRFSTL (L) 9 SYSLEKISSL S/Y|P R/R\F S|T|L 55.6 5 4 5 3 0 dual b/y bias 0.999 99.5 31.83 55.03 0.0 563.8035 1126.6 -0.0007 -0.6 -1.1 10.83 5911.7 smORF_v4|nuORFdb_human IFRD1_7:112090542-112090700:+ IFRD1_7:112090542-112090700:+|ENST00000403825.7_1_7:112090541-112090697:+ IFRD1 uORF 7:112090542-112090700:+ 7 IFRD1 5' uORF 5' uORF GN=IFRD1 AL20210827_EV_HLA_PDAC_PANFRO413_2D_50e6_W632_classI_fxn3 Exploris AL20221201_MKP_PDAC_01.20525.20781.2 2 1 1 14.65 9.16 6.02 5.49 88.0 6 1 2 1140000000.0 Hydrogen 20 (H) 9 SYPRRFSTL (L) 9 SYREPKHPL S/Y|P R/R\F S|T|L 55.6 5 4 5 3 0 dual b/y bias 0.999 96.2 35.57 100.0 0.0 563.8041 1126.6 0.0006 0.6 -0.20000000000000007 10.83 5911.7 smORF_v4|nuORFdb_human IFRD1_7:112090542-112090700:+ IFRD1_7:112090542-112090700:+|ENST00000403825.7_1_7:112090541-112090697:+ IFRD1 uORF 7:112090542-112090700:+ 7 IFRD1 5' uORF 5' uORF GN=IFRD1 AL20221201_MKP_PDAC_01 SCP E_20221201_NO30_400nL_HLAc1_4e7_PDAC_Slot2-6_1_3524.9176.1321.2 2 2 1 12.77 7.45 6.08 5.33 82.9 7 1 2 257000.0 Hydrogen 20 (H) 9 SYPRRFSTL (L) 9 SYKRLAmEL S/Y/P R/R\F\S\T\L 77.8 7 4 3 0 0 unbiased 31.700142 0.925833 563.803 1126.6 -0.0016 -1.5 0.8999999999999999 10.83 5911.7 smORF_v4|nuORFdb_human IFRD1_7:112090542-112090700:+ IFRD1_7:112090542-112090700:+|ENST00000403825.7_1_7:112090541-112090697:+ IFRD1 uORF 7:112090542-112090700:+ 7 IFRD1 5' uORF 5' uORF GN=IFRD1 E_20221201_NO30_400nL_HLAc1_4e7_PDAC_Slot2-6_1_3524 SCP E_20221201_NO30_400nL_HLAc1_4e7_PDAC_Slot2-6_1_3524.21923.488.1 1 2 1 13.45 6.94 4.36 6.51 84.6 7 0 0 18200.0 Hydrogen 84 (S) 9 SYSGVTQLL (R) 9 MPQHTGALL S Y/S/G\V|T|Q\L\L 77.8 7 5 4 2 0 unbiased 75.737206 1.432922 967.5081 967.51 -0.0015 -1.5 0.8999999999999999 5.24 14478.4 nuORFdb_human ENST00000511767.3_1_4:95376439-95471092:+ ENST00000511767.3_1_4:95376439-95471092:+ PDLIM5 5' Overlap uORF 5' Overlap uORF GN=PDLIM5 5' Overlap uORF E_20221201_NO30_400nL_HLAc1_4e7_PDAC_Slot2-6_1_3524 10mio-rep1 E_20221201_NO30_400nL_HLAc1_1e7_directIP_titration_rep1_Slot2-3_1_3512.12491.555.1 1 1 1 12.33 6.15 6.52 6.17 82.6 8 0 0 3940.0 Hydrogen 10 (R) 11 TAAAAAGAASW (A) 11 KAADGAAASnA T A/A A/A/A|G\A\A\S\W 63.6 7 5 4 1 0 unbiased 43.153461 1.393283 947.451 947.458 -0.0071 -7.5 0.20000000000000018 5.18 6116.0 smORF_v4|nuORFdb_human URI1_19:30433277-30433438:+ URI1_19:30433277-30433438:+|ENST00000574110.5_1_19:30433210-30433438:+ URI1 uORF 19:30433277-30433438:+ 19 URI1 5' uORF 5' uORF GN=URI1 E_20221201_NO30_400nL_HLAc1_1e7_directIP_titration_rep1_Slot2-3_1_3512 10mio-rep2 E_20221201_NO30_400nL_HLAc1_1e7_directIP_titration_rep2_Slot2-3_1_3513.12458.551.1 1 2 1 15.12 8.17 8.27 6.95 88.3 8 0 0 6460.0 Hydrogen 10 (R) 11 TAAAAAGAASW (A) 11 KAADGAAASnA T A A/A/A|A|G\A\A\S\W 72.7 8 6 4 2 0 unbiased 43.040607 1.395474 947.4542 947.458 -0.0041 -4.3 2.4000000000000004 5.18 6116.0 smORF_v4|nuORFdb_human URI1_19:30433277-30433438:+ URI1_19:30433277-30433438:+|ENST00000574110.5_1_19:30433210-30433438:+ URI1 uORF 19:30433277-30433438:+ 19 URI1 5' uORF 5' uORF GN=URI1 E_20221201_NO30_400nL_HLAc1_1e7_directIP_titration_rep2_Slot2-3_1_3513 10mio-rep3 E_20221201_NO30_400nL_HLAc1_1e7_directIP_titration_rep3_Slot2-3_1_3514.12436.555.1 1 2 1 15.94 7.18 8.74 8.76 90.6 9 0 0 7140.0 Hydrogen 10 (R) 11 TAAAAAGAASW (A) 11 KAADGAAASnA T A/A/A/A|A|G\A\A\S\W 81.8 9 6 5 2 0 unbiased 42.966938 1.393282 947.4528 947.458 -0.0051 -5.4 0.8999999999999995 5.18 6116.0 smORF_v4|nuORFdb_human URI1_19:30433277-30433438:+ URI1_19:30433277-30433438:+|ENST00000574110.5_1_19:30433210-30433438:+ URI1 uORF 19:30433277-30433438:+ 19 URI1 5' uORF 5' uORF GN=URI1 E_20221201_NO30_400nL_HLAc1_1e7_directIP_titration_rep3_Slot2-3_1_3514 20mio-rep1 E_20221201_NO30_400nL_HLAc1_2e7_directIP_titration_rep1_Slot2-4_1_3515.12409.1577.2 2 3 1 16.95 4.26 13.14 12.69 88.3 9 0 0 11500.0 Hydrogen 10 (R) 11 TAAAAAGAASW (A) 11 YAGAAGGVAGPG T A/A/A|A|A/G\A|A|S\W 81.8 9 6 7 4 0 dual b/y bias 42.861023 0.765177 474.228 947.458 -0.0094 -9.9 -3.7 5.18 6116.0 smORF_v4|nuORFdb_human URI1_19:30433277-30433438:+ URI1_19:30433277-30433438:+|ENST00000574110.5_1_19:30433210-30433438:+ URI1 uORF 19:30433277-30433438:+ 19 URI1 5' uORF 5' uORF GN=URI1 E_20221201_NO30_400nL_HLAc1_2e7_directIP_titration_rep1_Slot2-4_1_3515 20mio-rep2 E_20221201_NO30_400nL_HLAc1_2e7_directIP_titration_rep3_Slot2-4_1_3518.12546.1571.2 2 2 1 15.5 5.25 11.57 10.24 83.2 9 0 0 9560.0 Hydrogen 10 (R) 11 TAAAAAGAASW (A) 11 KAADGAAASnA T A/A/A|A|A/G\A\A|S\W 81.8 9 6 6 3 0 unbiased 43.335011 0.768598 474.2299 947.458 -0.0054 -5.7 0.5999999999999996 5.18 6116.0 smORF_v4|nuORFdb_human URI1_19:30433277-30433438:+ URI1_19:30433277-30433438:+|ENST00000574110.5_1_19:30433210-30433438:+ URI1 uORF 19:30433277-30433438:+ 19 URI1 5' uORF 5' uORF GN=URI1 E_20221201_NO30_400nL_HLAc1_2e7_directIP_titration_rep3_Slot2-4_1_3518 20mio-rep3 E_20221201_NO30_400nL_HLAc1_2e7_directIP_titration_rep4_Slot2-4_1_3519.12431.1576.2 2 3 1 16.06 7.11 9.69 8.95 87.2 9 0 0 10900.0 Hydrogen 10 (R) 11 TAAAAAGAASW (A) 11 KAADGAAASnA T A/A/A/A|A/G\A|A|S\W 81.8 9 5 7 3 0 unbiased 42.94117 0.765208 474.23 947.458 -0.0054 -5.7 0.09999999999999964 5.18 6116.0 smORF_v4|nuORFdb_human URI1_19:30433277-30433438:+ URI1_19:30433277-30433438:+|ENST00000574110.5_1_19:30433210-30433438:+ URI1 uORF 19:30433277-30433438:+ 19 URI1 5' uORF 5' uORF GN=URI1 E_20221201_NO30_400nL_HLAc1_2e7_directIP_titration_rep4_Slot2-4_1_3519 40mio-rep1 E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep1_Slot1-04_1_3496.12662.526.1 1 3 1 15.02 6.57 7.55 8.45 85.2 9 0 0 60800.0 Hydrogen 10 (R) 11 TAAAAAGAASW (A) 11 ATGAGGIGSAEG T A/A/A|A|A|G\A\A\S\W 81.8 9 7 5 3 0 unbiased 43.739094 1.410395 947.4553 947.458 -0.0031 -3.3 -0.09999999999999964 5.18 6116.0 smORF_v4|nuORFdb_human URI1_19:30433277-30433438:+ URI1_19:30433277-30433438:+|ENST00000574110.5_1_19:30433210-30433438:+ URI1 uORF 19:30433277-30433438:+ 19 URI1 5' uORF 5' uORF GN=URI1 E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep1_Slot1-04_1_3496 40mio-rep2 E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep2_Slot2-5_1_3520.12376.542.1 1 3 1 15.52 7.17 7.66 8.35 85.2 9 0 0 14800.0 Hydrogen 10 (R) 11 TAAAAAGAASW (A) 11 KAADGAAASnA T A/A/A|A|A|G\A\A\S\W 81.8 9 7 5 3 0 unbiased 42.753639 1.401201 947.4537 947.458 -0.0041 -4.3 0.6000000000000005 5.18 6116.0 smORF_v4|nuORFdb_human URI1_19:30433277-30433438:+ URI1_19:30433277-30433438:+|ENST00000574110.5_1_19:30433210-30433438:+ URI1 uORF 19:30433277-30433438:+ 19 URI1 5' uORF 5' uORF GN=URI1 E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep2_Slot2-5_1_3520 40mio-rep3 E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep3_Slot2-5_1_3521.12384.543.1 1 3 1 15.82 7.54 9.35 8.28 84.2 9 0 0 26900.0 Hydrogen 10 (R) 11 TAAAAAGAASW (A) 11 KAADGAAASnA T A/A/A|A|A|G\A\A\S\W 81.8 9 7 5 3 0 unbiased 42.785469 1.400189 947.4499 947.458 -0.0081 -8.6 -4.0 5.18 6116.0 smORF_v4|nuORFdb_human URI1_19:30433277-30433438:+ URI1_19:30433277-30433438:+|ENST00000574110.5_1_19:30433210-30433438:+ URI1 uORF 19:30433277-30433438:+ 19 URI1 5' uORF 5' uORF GN=URI1 E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep3_Slot2-5_1_3521 40mio-rep4 E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep4_Slot2-5_1_3522.12399.540.1 1 3 1 16.16 6.43 9.44 9.73 88.8 9 0 0 31300.0 Hydrogen 10 (R) 11 TAAAAAGAASW (A) 11 KAADGAAASnA T A/A/A/A|A|G\A\A\S\W 81.8 9 6 5 2 0 unbiased 42.818413 1.402074 947.4546 947.458 -0.0031 -3.3 1.4000000000000004 5.18 6116.0 smORF_v4|nuORFdb_human URI1_19:30433277-30433438:+ URI1_19:30433277-30433438:+|ENST00000574110.5_1_19:30433210-30433438:+ URI1 uORF 19:30433277-30433438:+ 19 URI1 5' uORF 5' uORF GN=URI1 E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep4_Slot2-5_1_3522 5mio-rep1 E_20221201_NO30_400nL_HLAc1_5e6_directIP_titration_rep1_Slot1-37_1_3502.12723.542.1 1 1 1 15.74 7.18 9.64 8.56 89.5 8 0 0 3560.0 Hydrogen 10 (R) 11 TAAAAAGAASW (A) 11 KAADGAAASnA T A A/A/A|A|G\A\A\S\W 72.7 8 6 4 2 0 unbiased 43.949734 1.401095 947.4548 947.458 -0.0031 -3.3 3.7 5.18 6116.0 smORF_v4|nuORFdb_human URI1_19:30433277-30433438:+ URI1_19:30433277-30433438:+|ENST00000574110.5_1_19:30433210-30433438:+ URI1 uORF 19:30433277-30433438:+ 19 URI1 5' uORF 5' uORF GN=URI1 E_20221201_NO30_400nL_HLAc1_5e6_directIP_titration_rep1_Slot1-37_1_3502 5mio-rep2 E_20221201_NO30_400nL_HLAc1_5e6_directIP_titration_rep2_Slot2-2_1_3510.12668.558.1 1 1 1 14.11 8.12 7.47 5.99 85.3 8 0 0 3140.0 Hydrogen 10 (R) 11 TAAAAAGAASW (A) 11 KAADGAAASnA T A/A A/A|A|G\A\A\S\W 63.6 7 6 4 2 0 unbiased 43.754417 1.39151 947.4521 947.458 -0.0061 -6.5 2.1999999999999993 5.18 6116.0 smORF_v4|nuORFdb_human URI1_19:30433277-30433438:+ URI1_19:30433277-30433438:+|ENST00000574110.5_1_19:30433210-30433438:+ URI1 uORF 19:30433277-30433438:+ 19 URI1 5' uORF 5' uORF GN=URI1 E_20221201_NO30_400nL_HLAc1_5e6_directIP_titration_rep2_Slot2-2_1_3510 5mio-rep3 E_20221201_NO30_400nL_HLAc1_5e6_directIP_titration_rep3_Slot2-2_1_3511.12692.555.1 1 1 1 14.57 8.27 8.53 6.3 86.2 8 0 0 2690.0 Hydrogen 10 (R) 11 TAAAAAGAASW (A) 11 KAADGAAASnA T A/A A/A|A|G\A\A\S\W 63.6 7 6 4 2 0 unbiased 43.844971 1.39294 947.4485 947.458 -0.0091 -9.6 -1.4000000000000004 5.18 6116.0 smORF_v4|nuORFdb_human URI1_19:30433277-30433438:+ URI1_19:30433277-30433438:+|ENST00000574110.5_1_19:30433210-30433438:+ URI1 uORF 19:30433277-30433438:+ 19 URI1 5' uORF 5' uORF GN=URI1 E_20221201_NO30_400nL_HLAc1_5e6_directIP_titration_rep3_Slot2-2_1_3511 40mio-rep1 E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep1_Slot1-04_1_3496.17677.446.1 1 2 1 9.44 3.43 5.9 6.01 70.5 7 0 0 6590.0 M45m Hydrogen 41 (R) 10 TAAGmEGLSW (L) 10 SLFQDQnW T A/A/G m|E\G\L\S\W 60.0 6 5 3 1 0 unbiased m:Oxidized methionine 61.063255 1.457919 1038.4536 1022.461 15.9888 -5.8 -2.5999999999999996 4.0 5582.4 nuORFdb_human ENST00000378119.8_1_6:24720562-24720727:- ENST00000378119.8_1_6:24720562-24720727:- C6orf62 5' uORF 5' uORF GN=C6orf62 5' uORF E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep1_Slot1-04_1_3496 20mio-rep1 E_20221201_NO30_400nL_HLAc1_2e7_directIP_titration_rep1_Slot2-4_1_3515.6216.1551.2 2 3 1 7.35 5.37 1.98 1.98 70.4 5 1 1 10900.0 Hydrogen 34 (T) 9 TAAGSPRTW (L) 9 TAAGQAPFSP T/A/A/G/S/P R T W 55.6 5 0 5 0 0 y-ion bias 21.47772 0.781412 473.7355 946.474 -0.0094 -9.9 -3.7 9.41 5957.7 nuORFdb_human ENST00000484515.1_1_20:4158069-4162462:+ ENST00000484515.1_1_20:4158069-4162462:+ SMOX ncRNA Processed Transcript lncRNA GN=SMOX ncRNA Processed Transcript E_20221201_NO30_400nL_HLAc1_2e7_directIP_titration_rep1_Slot2-4_1_3515 20mio-rep2 E_20221201_NO30_400nL_HLAc1_2e7_directIP_titration_rep3_Slot2-4_1_3518.6337.1551.2 2 1 1 7.72 4.72 2.51 3.0 75.2 6 1 1 3370.0 Hydrogen 34 (T) 9 TAAGSPRTW (L) 9 TACVLTGQP T/A/A/G/S/P R T\W 66.7 6 1 5 0 0 y-ion bias 21.897163 0.781286 473.7373 946.474 -0.0074 -7.8 -1.5 9.41 5957.7 nuORFdb_human ENST00000484515.1_1_20:4158069-4162462:+ ENST00000484515.1_1_20:4158069-4162462:+ SMOX ncRNA Processed Transcript lncRNA GN=SMOX ncRNA Processed Transcript E_20221201_NO30_400nL_HLAc1_2e7_directIP_titration_rep3_Slot2-4_1_3518 20mio-rep3 E_20221201_NO30_400nL_HLAc1_2e7_directIP_titration_rep4_Slot2-4_1_3519.6259.1567.2 2 1 1 7.96 6.19 1.72 1.76 74.5 5 1 1 5400.0 Hydrogen 34 (T) 9 TAAGSPRTW (L) 9 TAAGQAPFSP T/A/A|G|S|P R T W 55.6 5 3 5 3 0 dual b/y bias 21.625128 0.771074 473.737 946.474 -0.0074 -7.8 -2.0 9.41 5957.7 nuORFdb_human ENST00000484515.1_1_20:4158069-4162462:+ ENST00000484515.1_1_20:4158069-4162462:+ SMOX ncRNA Processed Transcript lncRNA GN=SMOX ncRNA Processed Transcript E_20221201_NO30_400nL_HLAc1_2e7_directIP_titration_rep4_Slot2-4_1_3519 40mio-rep1 E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep1_Slot1-04_1_3496.6457.1544.2 2 1 1 11.01 8.63 2.92 2.38 83.7 6 1 1 8200.0 Hydrogen 34 (T) 9 TAAGSPRTW (L) 9 TAAGQAPFSP T/A/A|G|S/P R/T W 55.6 5 2 6 2 0 y-ion bias 22.313425 0.785976 473.7386 946.474 -0.0034 -3.6 -0.3999999999999999 9.41 5957.7 nuORFdb_human ENST00000484515.1_1_20:4158069-4162462:+ ENST00000484515.1_1_20:4158069-4162462:+ SMOX ncRNA Processed Transcript lncRNA GN=SMOX ncRNA Processed Transcript E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep1_Slot1-04_1_3496 40mio-rep2 E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep2_Slot2-5_1_3520.6200.1531.2 2 1 1 9.85 6.89 2.4 2.96 80.2 5 1 1 7460.0 Hydrogen 34 (T) 9 TAAGSPRTW (L) 9 TAAGQAPFSP T/A/A|G/S/P R T W 55.6 5 1 5 1 0 y-ion bias 21.425631 0.793724 473.7374 946.474 -0.0074 -7.8 -2.8999999999999995 9.41 5957.7 nuORFdb_human ENST00000484515.1_1_20:4158069-4162462:+ ENST00000484515.1_1_20:4158069-4162462:+ SMOX ncRNA Processed Transcript lncRNA GN=SMOX ncRNA Processed Transcript E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep2_Slot2-5_1_3520 40mio-rep3 E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep3_Slot2-5_1_3521.6211.1532.2 2 1 1 7.16 5.06 2.1 2.1 70.2 5 1 1 8140.0 Hydrogen 34 (T) 9 TAAGSPRTW (L) 9 TACVLTGQP T/A/A/G|S|P R T W 55.6 5 2 5 2 0 y-ion bias 21.458483 0.793023 473.7385 946.474 -0.0034 -3.6 0.9999999999999996 9.41 5957.7 nuORFdb_human ENST00000484515.1_1_20:4158069-4162462:+ ENST00000484515.1_1_20:4158069-4162462:+ SMOX ncRNA Processed Transcript lncRNA GN=SMOX ncRNA Processed Transcript E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep3_Slot2-5_1_3521 SCP E_20221201_NO30_400nL_HLAc1_4e7_PDAC_Slot2-6_1_3524.8849.572.1 1 2 1 11.62 7.39 9.24 4.23 69.4 7 0 0 10800.0 Hydrogen 21 (R) 9 TAAGTSVCY (R) 9 SNLSSVCY T A/A/G/T|S|V|C\Y 77.8 7 4 6 3 0 dual b/y bias Carbamidomethylation C:Carbamidomethylation, 30.570648 1.382866 929.3991 929.403 -0.0043 -4.6 -2.1999999999999997 5.19 4939.5 smORF_v4|nuORFdb_human MARCHF3_5:126366042-126253919:- MARCHF3_5:126366042-126253919:-|ENST00000308660.5_1_5:126253889-126366245:- MARCHF3 uORF 5:126366042-126253919:- 5 MARCH3 5' uORF 5' uORF GN=MARCHF3 E_20221201_NO30_400nL_HLAc1_4e7_PDAC_Slot2-6_1_3524 SCP E_20221201_NO30_400nL_HLAc1_4e7_PDAC_Slot2-6_1_3524.13474.683.1 1 2 1 9.9 6.81 3.35 3.09 70.0 7 0 0 29700.0 Hydrogen 50 (A) 9 TAAIATSSL (S) 9 VSGITASSL T A/A|I|A|T\S\S\L 77.8 7 6 4 3 0 dual b/y bias 46.549313 1.316582 834.4543 834.457 -0.0027 -3.3 -0.8999999999999999 5.18 6674.7 nuORFdb_human ENST00000246672.3_1_17:38256375-38256564:- ENST00000246672.3_1_17:38256375-38256564:- NR1D1 5' uORF 5' uORF GN=NR1D1 5' uORF E_20221201_NO30_400nL_HLAc1_4e7_PDAC_Slot2-6_1_3524 SCP E_20221201_NO30_400nL_HLAc1_4e7_PDAC_Slot2-6_1_3524.12446.663.1 1 1 1 12.27 7.01 6.37 5.26 81.2 7 0 0 8860.0 Hydrogen 148 (T) 9 TAASVTDSL (G) 9 TAASSLnTV T A/A/S|V|T\D\S\L 77.8 7 5 4 2 0 unbiased 42.996639 1.328363 864.4308 864.431 0.0001 0.1 2.5 3.8 18898.3 nuORFdb_human ENST00000469390.5_1_1:156876157-156877908:+ ENST00000469390.5_1_1:156876157-156877908:+|ENST00000338302.7_1_1:156876157-156878457:+ PEAR1 3' Overlap dORF 3' Overlap dORF GN=PEAR1 3' Overlap dORF E_20221201_NO30_400nL_HLAc1_4e7_PDAC_Slot2-6_1_3524 Eva-50mio-3fr AL20210827_EV_HLA_PDAC_PANFRO413_2D_50e6_W632_classI_fxn2.33780.33780.2 2 1 1 13.09 4.75 8.89 8.33 80.9 8 1 1 4370000.0 Hydrogen 78 (L) 9 TADSTIFKF (V) 9 TAKFDTSFL T/A|D|S\T-I/F/K/F 100.0 9 3 6 2 1 y-ion bias 0.979 69.0 62.0 100.0 0.0 515.266 1029.525 -0.0003 -0.3 -0.6 5.5 21306.4 nuORFdb_human ENST00000431677.1_1_X:100673784-100702742:+ ENST00000431677.1_1_X:100673784-100702742:+|ENST00000433011.5_1_X:100699118-100701032:+ ARMCX4 ncRNA Processed Transcript lncRNA GN=ARMCX4 ncRNA Processed Transcript AL20210827_EV_HLA_PDAC_PANFRO413_2D_50e6_W632_classI_fxn2 SCP E_20221201_NO30_400nL_HLAc1_4e7_PDAC_Slot2-6_1_3524.19735.1456.2 2 2 1 16.23 2.56 12.88 13.67 81.9 8 1 1 16800.0 Hydrogen 78 (L) 9 TADSTIFKF (V) 9 SVVTDSIHAT T/A/D|S|T|I|F|K\F 100.0 9 6 7 5 0 dual b/y bias 68.177368 0.841029 515.2631 1029.525 -0.0064 -6.2 -3.8000000000000003 5.5 21306.4 nuORFdb_human ENST00000431677.1_1_X:100673784-100702742:+ ENST00000431677.1_1_X:100673784-100702742:+|ENST00000433011.5_1_X:100699118-100701032:+ ARMCX4 ncRNA Processed Transcript lncRNA GN=ARMCX4 ncRNA Processed Transcript E_20221201_NO30_400nL_HLAc1_4e7_PDAC_Slot2-6_1_3524 10mio-rep3 E_20221201_NO30_400nL_HLAc1_1e7_directIP_titration_rep3_Slot2-3_1_3514.13074.1462.2 2 1 1 7.54 3.5 5.67 4.04 57.0 7 0 0 7620.0 Hydrogen 25 (H) 9 TADVHGLYY (T) 9 TADGEPSAKY T/A/D\V H\G\L|Y\Y 77.8 7 5 3 1 0 unbiased 45.169285 0.837496 519.7466 1038.489 -0.0024 -2.3 4.0 5.05 7536.3 nuORFdb_human ENST00000476493.5_1_1:33099602-33116090:- ENST00000476493.5_1_1:33099602-33116090:- ZBTB8OS 3' dORF 3' dORF GN=ZBTB8OS 3' dORF E_20221201_NO30_400nL_HLAc1_1e7_directIP_titration_rep3_Slot2-3_1_3514 40mio-rep1 E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep1_Slot1-04_1_3496.13359.1419.2 2 1 1 11.82 5.37 5.27 6.44 81.7 8 0 0 30800.0 Hydrogen 25 (H) 9 TADVHGLYY (T) 9 EnAKLHYY T/A/D\V/H-G\L|Y\Y 100.0 9 4 4 1 1 unbiased 46.146347 0.864463 519.7474 1038.489 -0.0024 -2.3 0.9000000000000004 5.05 7536.3 nuORFdb_human ENST00000476493.5_1_1:33099602-33116090:- ENST00000476493.5_1_1:33099602-33116090:- ZBTB8OS 3' dORF 3' dORF GN=ZBTB8OS 3' dORF E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep1_Slot1-04_1_3496 5mio-rep3 E_20221201_NO30_400nL_HLAc1_5e6_directIP_titration_rep3_Slot2-2_1_3511.13344.1473.2 2 1 1 10.01 6.6 6.04 3.42 64.8 7 0 0 2330.0 Hydrogen 25 (H) 9 TADVHGLYY (T) 9 TADGEPSAKY T/A/D/V H\G\L|Y\Y 77.8 7 4 4 1 0 unbiased 46.09581 0.830649 519.7456 1038.489 -0.0044 -4.2 3.999999999999999 5.05 7536.3 nuORFdb_human ENST00000476493.5_1_1:33099602-33116090:- ENST00000476493.5_1_1:33099602-33116090:- ZBTB8OS 3' dORF 3' dORF GN=ZBTB8OS 3' dORF E_20221201_NO30_400nL_HLAc1_5e6_directIP_titration_rep3_Slot2-2_1_3511 SCP E_20221201_NO30_400nL_HLAc1_4e7_PDAC_Slot2-6_1_3524.25093.500.1 1 4 1 8.6 3.5 5.43 5.1 61.0 6 0 0 204000.0 Hydrogen 9 (S) 9 TAFSTVVGF (-) 9 VGTSLTFGF T A F\S|T\V\V\G\F 66.7 6 6 1 1 0 b-ion bias 86.68882 1.425842 928.4761 928.477 -0.0015 -1.6 0.7999999999999998 5.18 1882.2 nuORFdb_human ENST00000392750.6_2_3:167437948-167437999:- ENST00000392750.6_2_3:167437948-167437999:- PDCD10 5' uORF 5' uORF GN=PDCD10 5' uORF E_20221201_NO30_400nL_HLAc1_4e7_PDAC_Slot2-6_1_3524 Exploris_FAIMS VH20221207_MKP_FAIMS2022E_HLAc1_4e7_PDAC.59485.59622.2 2 1 1 7.11 4.48 2.48 2.63 63.3 6 0 0 25900000.0 Hydrogen 9 (S) 9 TAFSTVVGF (-) 9 FGVHPAPGF T A\F\S-T V-V/G/F 55.6 5 2 2 0 2 unbiased 0.999 99.5 103.42 37.37 -70.0 464.7412 928.477 -0.0023 -2.5 0.10000000000000009 5.18 1882.2 nuORFdb_human ENST00000392750.6_2_3:167437948-167437999:- ENST00000392750.6_2_3:167437948-167437999:- PDCD10 5' uORF 5' uORF GN=PDCD10 5' uORF VH20221207_MKP_FAIMS2022E_HLAc1_4e7_PDAC Eva-50mio-3fr AL20210827_EV_HLA_PDAC_PANFRO413_2D_50e6_W632_classI_fxn1.35288.35288.1 1 1 1 9.7 3.05 8.59 6.65 73.1 6 0 0 20700000.0 Hydrogen 4 (A) 9 TAGESFIVY (Q) 9 FSATnALYV T A\G\E\S-F\I\V Y 55.6 5 5 0 0 1 b-ion bias 0.795 100.0 62.93 100.0 0.0 986.4833 986.483 0.0003 0.3 0.19999999999999998 4.0 2341.7 nuORFdb_human ENST00000475946.5_1_6:7181423-7181483:+ ENST00000475946.5_1_6:7181423-7181483:+|ENST00000379938.6_3_6:7181423-7182124:+ RREB1 ncRNA Retained Intron lncRNA GN=RREB1 ncRNA Retained Intron AL20210827_EV_HLA_PDAC_PANFRO413_2D_50e6_W632_classI_fxn1 SCP E_20221201_NO30_400nL_HLAc1_4e7_PDAC_Slot2-6_1_3524.20913.439.1 1 3 1 10.49 5.62 4.86 4.87 79.0 5 0 0 57800.0 Hydrogen 4 (A) 9 TAGESFIVY (Q) 9 ERCFLVY T A G E|S/F\I\V\Y 55.6 5 4 2 1 0 b-ion bias 72.245956 1.462337 986.4812 986.483 -0.0019 -2.0 0.3999999999999999 4.0 2341.7 nuORFdb_human ENST00000475946.5_1_6:7181423-7181483:+ ENST00000475946.5_1_6:7181423-7181483:+|ENST00000379938.6_3_6:7181423-7182124:+ RREB1 ncRNA Retained Intron lncRNA GN=RREB1 ncRNA Retained Intron E_20221201_NO30_400nL_HLAc1_4e7_PDAC_Slot2-6_1_3524 Exploris_FAIMS VH20221207_MKP_FAIMS2022E_HLAc1_4e7_PDAC.52097.52097.2 2 1 1 8.96 8.96 8.96 50.6 7 0 0 66400000.0 Hydrogen 4 (A) 9 TAGESFIVY (Q) 9 T A\G\E\S-F\I/V/Y 77.8 7 4 2 0 1 b-ion bias 0.792 87.9 91.73 107.63 -70.0 493.744 986.483 -0.0022 -2.3 0.30000000000000027 4.0 2341.7 nuORFdb_human ENST00000475946.5_1_6:7181423-7181483:+ ENST00000475946.5_1_6:7181423-7181483:+|ENST00000379938.6_3_6:7181423-7182124:+ RREB1 ncRNA Retained Intron lncRNA GN=RREB1 ncRNA Retained Intron VH20221207_MKP_FAIMS2022E_HLAc1_4e7_PDAC SCP E_20221201_NO30_400nL_HLAc1_4e7_PDAC_Slot2-6_1_3524.15141.636.1 1 1 1 12.17 4.29 6.51 7.89 79.9 7 0 0 7830.0 Hydrogen 43 (P) 9 TAGPATPAW (A) 9 CLVTAPGGP T A/G/P\A|T|P\A\W 77.8 7 5 4 2 0 unbiased 52.309578 1.344474 871.4271 871.431 -0.0038 -4.4 -2.0000000000000004 5.18 5970.8 nuORFdb_human ENST00000591651.5_1_17:74373428-74381645:+ ENST00000591651.5_1_17:74373428-74381645:+|ENST00000545180.5_2_17:74377727-74381645:+|ENST00000588682.5_2_17:74377987-74381645:+|ENST00000591762.1_1_17:74379601-74381645:+|ENST00000392496.3_2_17:74381489-74381645:+|ENST00000592299.5_2_17:74380737-74381645:+|ENST00000323374.8_2_17:74380737-74381645:+ SPHK1 5' Overlap uORF 5' Overlap uORF GN=SPHK1 5' Overlap uORF E_20221201_NO30_400nL_HLAc1_4e7_PDAC_Slot2-6_1_3524 SCP E_20221201_NO30_400nL_HLAc1_4e7_PDAC_Slot2-6_1_3524.12063.722.1 1 1 1 11.55 5.91 7.3 5.64 90.4 8 0 0 7340.0 Hydrogen 42 (S) 9 TAGPGPVSM (G) 9 TAGPPAMAT T/A/G/P/G/P\V\S\M 100.0 9 3 5 0 0 unbiased 41.673702 1.292934 816.3896 816.392 -0.002 -2.5 -0.10000000000000009 5.18 7235.8 nuORFdb_human ENST00000371648.4_1_9:139756981-139757203:- ENST00000371648.4_1_9:139756981-139757203:- EDF1 3' dORF 3' dORF GN=EDF1 3' dORF E_20221201_NO30_400nL_HLAc1_4e7_PDAC_Slot2-6_1_3524 40mio-rep1 E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep1_Slot1-04_1_3496.10652.1510.2 2 2 1 12.59 8.27 3.92 4.32 79.2 6 1 1 17100.0 M72m Hydrogen 70 (C) 8 TAmRSIAW (-) 8 LRGTmTAW T/A/m/R S\I|A\W 75.0 6 3 4 1 0 unbiased m:Oxidized methionine 36.79818 0.806964 476.2365 935.477 15.99 -5.2 -2.0 9.41 8441.5 nuORFdb_human ENST00000429204.5_1_1:226925125-226925356:- ENST00000429204.5_1_1:226925125-226925356:- ITPKB 5' Overlap uORF 5' Overlap uORF GN=ITPKB 5' Overlap uORF E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep1_Slot1-04_1_3496 40mio-rep2 E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep2_Slot2-5_1_3520.10363.1523.2 2 2 1 9.3 6.44 3.92 2.86 68.3 6 1 1 14200.0 M72m Hydrogen 70 (C) 8 TAmRSIAW (-) 8 QFISRDW T/A/m/R S\I|A\W 75.0 6 3 4 1 0 unbiased m:Oxidized methionine 35.800945 0.798826 476.2382 935.477 15.992 -3.1 1.8000000000000003 9.41 8441.5 nuORFdb_human ENST00000429204.5_1_1:226925125-226925356:- ENST00000429204.5_1_1:226925125-226925356:- ITPKB 5' Overlap uORF 5' Overlap uORF GN=ITPKB 5' Overlap uORF E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep2_Slot2-5_1_3520 40mio-rep3 E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep3_Slot2-5_1_3521.10367.1531.2 2 1 1 8.39 5.09 3.79 3.3 61.6 6 1 1 8860.0 M72m Hydrogen 70 (C) 8 TAmRSIAW (-) 8 TISRMSAW T/A/m/R S\I|A\W 75.0 6 3 4 1 0 unbiased m:Oxidized methionine 35.817303 0.793687 476.2351 935.477 15.986 -9.4 -4.800000000000001 9.41 8441.5 nuORFdb_human ENST00000429204.5_1_1:226925125-226925356:- ENST00000429204.5_1_1:226925125-226925356:- ITPKB 5' Overlap uORF 5' Overlap uORF GN=ITPKB 5' Overlap uORF E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep3_Slot2-5_1_3521 20mio-rep1 E_20221201_NO30_400nL_HLAc1_2e7_directIP_titration_rep1_Slot2-4_1_3515.13454.1554.2 2 2 1 11.08 6.18 3.81 4.9 81.8 6 1 1 13600.0 Hydrogen 3 (I) 9 TARVTVTGW (R) 9 ALTQLTTGW T A R|V/T\V|T|G\W 66.7 6 5 4 3 0 dual b/y bias 46.468971 0.779115 495.7665 990.537 -0.01 -10.1 -3.8999999999999995 9.41 2188.5 nuORFdb_human ENST00000456057.5_2_6:41755171-41755237:+ ENST00000456057.5_2_6:41755171-41755237:+ RP11-298J23.10 3' dORF 3' dORF GN=RP11-298J23.10 3' dORF E_20221201_NO30_400nL_HLAc1_2e7_directIP_titration_rep1_Slot2-4_1_3515 20mio-rep2 E_20221201_NO30_400nL_HLAc1_2e7_directIP_titration_rep3_Slot2-4_1_3518.13573.1554.2 2 2 1 10.74 5.63 3.78 5.11 87.4 6 1 1 9490.0 Hydrogen 3 (I) 9 TARVTVTGW (R) 9 KTVDALTGW T A/R V\T|V|T|G\W 55.6 5 5 4 3 0 dual b/y bias 46.880882 0.779363 495.7672 990.537 -0.01 -10.1 -3.8 9.41 2188.5 nuORFdb_human ENST00000456057.5_2_6:41755171-41755237:+ ENST00000456057.5_2_6:41755171-41755237:+ RP11-298J23.10 3' dORF 3' dORF GN=RP11-298J23.10 3' dORF E_20221201_NO30_400nL_HLAc1_2e7_directIP_titration_rep3_Slot2-4_1_3518 20mio-rep3 E_20221201_NO30_400nL_HLAc1_2e7_directIP_titration_rep4_Slot2-4_1_3519.13485.1557.2 2 1 1 10.16 5.16 3.4 5.0 83.2 6 1 1 5500.0 Hydrogen 3 (I) 9 TARVTVTGW (R) 9 ALTQLTTGW T A R/V\T|V|T|G\W 66.7 6 5 4 3 0 dual b/y bias 46.580448 0.777702 495.7687 990.537 -0.006 -6.0 -0.20000000000000018 9.41 2188.5 nuORFdb_human ENST00000456057.5_2_6:41755171-41755237:+ ENST00000456057.5_2_6:41755171-41755237:+ RP11-298J23.10 3' dORF 3' dORF GN=RP11-298J23.10 3' dORF E_20221201_NO30_400nL_HLAc1_2e7_directIP_titration_rep4_Slot2-4_1_3519 40mio-rep1 E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep1_Slot1-04_1_3496.13722.1538.2 2 1 1 16.67 6.34 5.9 10.33 93.0 7 1 1 6380.0 Hydrogen 3 (I) 9 TARVTVTGW (R) 9 GTLEKRTW T A/R|V|T|V|T|G|W 77.8 7 6 7 6 0 dual b/y bias 47.400021 0.789768 495.77 990.537 -0.004 -4.0 -0.7999999999999998 9.41 2188.5 nuORFdb_human ENST00000456057.5_2_6:41755171-41755237:+ ENST00000456057.5_2_6:41755171-41755237:+ RP11-298J23.10 3' dORF 3' dORF GN=RP11-298J23.10 3' dORF E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep1_Slot1-04_1_3496 40mio-rep2 E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep2_Slot2-5_1_3520.13455.1549.2 2 3 1 14.99 7.51 5.37 7.49 88.8 7 1 1 34500.0 Hydrogen 3 (I) 9 TARVTVTGW (R) 9 KTVDALTGW T A/R|V/T|V|T|G|W 77.8 7 5 7 5 0 dual b/y bias 46.479458 0.78283 495.7682 990.537 -0.008 -8.1 -3.1999999999999993 9.41 2188.5 nuORFdb_human ENST00000456057.5_2_6:41755171-41755237:+ ENST00000456057.5_2_6:41755171-41755237:+ RP11-298J23.10 3' dORF 3' dORF GN=RP11-298J23.10 3' dORF E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep2_Slot2-5_1_3520 40mio-rep3 E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep3_Slot2-5_1_3521.13446.1551.2 2 3 1 10.88 5.41 6.03 5.46 87.3 6 1 1 20600.0 Hydrogen 3 (I) 9 TARVTVTGW (R) 9 LVTTNVTWG T A/R|V T|V|T|G\W 55.6 5 5 5 4 0 dual b/y bias 46.452801 0.781612 495.768 990.537 -0.008 -8.1 -3.5 9.41 2188.5 nuORFdb_human ENST00000456057.5_2_6:41755171-41755237:+ ENST00000456057.5_2_6:41755171-41755237:+ RP11-298J23.10 3' dORF 3' dORF GN=RP11-298J23.10 3' dORF E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep3_Slot2-5_1_3521 40mio-rep4 E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep4_Slot2-5_1_3522.13441.1550.2 2 1 1 12.5 5.67 4.97 6.83 89.3 7 1 1 5370.0 Hydrogen 3 (I) 9 TARVTVTGW (R) 9 TVSQIISGW T A/R\V|T|V|T|G\W 77.8 7 6 5 4 0 dual b/y bias 46.416515 0.782231 495.7693 990.537 -0.006 -6.0 -1.2999999999999998 9.41 2188.5 nuORFdb_human ENST00000456057.5_2_6:41755171-41755237:+ ENST00000456057.5_2_6:41755171-41755237:+ RP11-298J23.10 3' dORF 3' dORF GN=RP11-298J23.10 3' dORF E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep4_Slot2-5_1_3522 40mio-rep3 E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep3_Slot2-5_1_3521.13749.1516.2 2 1 1 9.18 4.29 3.06 4.89 64.0 7 1 1 5480.0 Hydrogen 4 (R) 9 TAVGTTRIF (R) 9 PSAVGKPGVPG T/A/V|G/T/T R\I\F 77.8 7 3 5 1 0 unbiased 47.498592 0.803461 483.2707 965.542 -0.0067 -7.0 -2.4000000000000004 9.41 4007.5 nuORFdb_human ENST00000440460.6_1_11:111853129-111855981:+ ENST00000440460.6_1_11:111853129-111855981:+ DIXDC1 Out-of-Frame Out-of-Frame GN=DIXDC1 Out-of-Frame E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep3_Slot2-5_1_3521 40mio-rep4 E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep4_Slot2-5_1_3522.13749.1506.2 2 1 1 9.81 5.43 4.23 4.38 56.4 6 1 1 5500.0 Hydrogen 4 (R) 9 TAVGTTRIF (R) 9 TAVFLATSGV T/A/V|G/T|T-R\I F 77.8 7 3 5 2 1 unbiased 47.482422 0.809494 483.2706 965.542 -0.0067 -7.0 -2.3 9.41 4007.5 nuORFdb_human ENST00000440460.6_1_11:111853129-111855981:+ ENST00000440460.6_1_11:111853129-111855981:+ DIXDC1 Out-of-Frame Out-of-Frame GN=DIXDC1 Out-of-Frame E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep4_Slot2-5_1_3522 SCP E_20221201_NO30_400nL_HLAc1_4e7_PDAC_Slot2-6_1_3524.13966.574.1 1 2 1 10.6 6.19 5.41 4.41 83.6 6 0 0 17900.0 Hydrogen 80 (A) 9 TAVPTTPSM (A) 9 VLDAATPMS T A/V|P T/T|P\S\M 55.6 5 4 4 2 0 unbiased 48.248112 1.381535 904.4419 904.444 -0.0024 -2.7 -0.30000000000000027 5.18 15814.5 nuORFdb_human ENST00000329687.8_1_4:2934846-2935730:- ENST00000329687.8_1_4:2934846-2935730:- MFSD10 5' Overlap uORF 5' Overlap uORF GN=MFSD10 5' Overlap uORF E_20221201_NO30_400nL_HLAc1_4e7_PDAC_Slot2-6_1_3524 10mio-rep1 E_20221201_NO30_400nL_HLAc1_1e7_directIP_titration_rep1_Slot2-3_1_3512.20064.1361.2 2 1 1 12.45 2.62 10.01 9.82 69.4 9 1 1 3820.0 Hydrogen 11 (T) 11 TAVSNLVSRAW (-) 11 GFQPRVLVQC T A/V|S/N/L/V|S/R|A\W 81.8 9 4 8 3 0 y-ion bias 69.308739 0.900712 602.3215 1203.648 -0.0133 -11.1 -3.3999999999999995 9.41 2415.9 nuORFdb_human ENST00000343677.3_1_6:26056391-26056457:- ENST00000343677.3_1_6:26056391-26056457:- HIST1H1C Out-of-Frame Out-of-Frame GN=HIST1H1C Out-of-Frame E_20221201_NO30_400nL_HLAc1_1e7_directIP_titration_rep1_Slot2-3_1_3512 10mio-rep2 E_20221201_NO30_400nL_HLAc1_1e7_directIP_titration_rep2_Slot2-3_1_3513.19998.1372.2 2 2 1 14.66 2.9 11.93 11.76 79.5 9 1 1 7580.0 Hydrogen 11 (T) 11 TAVSNLVSRAW (-) 11 GFAILnMIQVP T A/V|S/N/L/V|S/R|A\W 81.8 9 4 8 3 0 y-ion bias 69.075447 0.893809 602.3228 1203.648 -0.0093 -7.7 -1.0 9.41 2415.9 nuORFdb_human ENST00000343677.3_1_6:26056391-26056457:- ENST00000343677.3_1_6:26056391-26056457:- HIST1H1C Out-of-Frame Out-of-Frame GN=HIST1H1C Out-of-Frame E_20221201_NO30_400nL_HLAc1_1e7_directIP_titration_rep2_Slot2-3_1_3513 10mio-rep3 E_20221201_NO30_400nL_HLAc1_1e7_directIP_titration_rep3_Slot2-3_1_3514.19939.1373.2 2 1 1 8.72 9.1 8.72 63.0 8 1 1 3200.0 Hydrogen 11 (T) 11 TAVSNLVSRAW (-) 11 T A V|S/N|L/V/S/R\A\W 72.7 8 4 6 2 0 unbiased 68.875877 0.89299 602.3233 1203.648 -0.0093 -7.7 -1.4000000000000004 9.41 2415.9 nuORFdb_human ENST00000343677.3_1_6:26056391-26056457:- ENST00000343677.3_1_6:26056391-26056457:- HIST1H1C Out-of-Frame Out-of-Frame GN=HIST1H1C Out-of-Frame E_20221201_NO30_400nL_HLAc1_1e7_directIP_titration_rep3_Slot2-3_1_3514 20mio-rep1 E_20221201_NO30_400nL_HLAc1_2e7_directIP_titration_rep1_Slot2-4_1_3515.20002.1354.2 2 2 1 17.75 6.02 11.66 11.72 87.6 9 1 1 16400.0 Hydrogen 11 (T) 11 TAVSNLVSRAW (-) 11 AVTSNRKEIW T A/V/S|N|L/V|S/R|A\W 81.8 9 5 8 4 0 dual b/y bias 69.075348 0.905341 602.3256 1203.648 -0.0033 -2.8 3.4000000000000004 9.41 2415.9 nuORFdb_human ENST00000343677.3_1_6:26056391-26056457:- ENST00000343677.3_1_6:26056391-26056457:- HIST1H1C Out-of-Frame Out-of-Frame GN=HIST1H1C Out-of-Frame E_20221201_NO30_400nL_HLAc1_2e7_directIP_titration_rep1_Slot2-4_1_3515 20mio-rep2 E_20221201_NO30_400nL_HLAc1_2e7_directIP_titration_rep3_Slot2-4_1_3518.20038.1352.2 2 3 1 16.46 4.2 13.45 12.26 83.3 9 1 1 46100.0 Hydrogen 11 (T) 11 TAVSNLVSRAW (-) 11 KETNLVMLQQ T A/V|S|N|L/V|S/R|A\W 81.8 9 6 8 5 0 dual b/y bias 69.215805 0.906567 602.3227 1203.648 -0.0093 -7.7 -1.4000000000000004 9.41 2415.9 nuORFdb_human ENST00000343677.3_1_6:26056391-26056457:- ENST00000343677.3_1_6:26056391-26056457:- HIST1H1C Out-of-Frame Out-of-Frame GN=HIST1H1C Out-of-Frame E_20221201_NO30_400nL_HLAc1_2e7_directIP_titration_rep3_Slot2-4_1_3518 20mio-rep3 E_20221201_NO30_400nL_HLAc1_2e7_directIP_titration_rep4_Slot2-4_1_3519.20100.1341.2 2 4 1 19.84 6.05 15.72 13.79 90.9 9 1 1 49600.0 Hydrogen 11 (T) 11 TAVSNLVSRAW (-) 11 AVTSNRKEIW T A/V|S|N/L/V|S/R|A\W 81.8 9 5 8 4 0 dual b/y bias 69.420631 0.912954 602.3245 1203.648 -0.0053 -4.4 1.3999999999999995 9.41 2415.9 nuORFdb_human ENST00000343677.3_1_6:26056391-26056457:- ENST00000343677.3_1_6:26056391-26056457:- HIST1H1C Out-of-Frame Out-of-Frame GN=HIST1H1C Out-of-Frame E_20221201_NO30_400nL_HLAc1_2e7_directIP_titration_rep4_Slot2-4_1_3519 40mio-rep1 E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep1_Slot1-04_1_3496.20243.1304.2 2 3 1 20.25 6.44 15.69 13.81 93.1 9 1 1 166000.0 Hydrogen 11 (T) 11 TAVSNLVSRAW (-) 11 AVTSNRKEIW T A/V|S|N|L/V|S/R|A\W 81.8 9 6 8 5 0 dual b/y bias 69.929657 0.936483 602.3266 1203.648 -0.0013 -1.1 2.1 9.41 2415.9 nuORFdb_human ENST00000343677.3_1_6:26056391-26056457:- ENST00000343677.3_1_6:26056391-26056457:- HIST1H1C Out-of-Frame Out-of-Frame GN=HIST1H1C Out-of-Frame E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep1_Slot1-04_1_3496 40mio-rep2 E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep2_Slot2-5_1_3520.20154.1330.2 2 3 1 20.89 7.65 16.58 13.25 94.3 9 1 1 117000.0 Hydrogen 11 (T) 11 TAVSNLVSRAW (-) 11 AVTSNRKEIW T A/V|S|N|L/V|S|R|A\W 81.8 9 7 8 6 0 dual b/y bias 69.622482 0.91998 602.3254 1203.648 -0.0053 -4.4 0.5 9.41 2415.9 nuORFdb_human ENST00000343677.3_1_6:26056391-26056457:- ENST00000343677.3_1_6:26056391-26056457:- HIST1H1C Out-of-Frame Out-of-Frame GN=HIST1H1C Out-of-Frame E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep2_Slot2-5_1_3520 40mio-rep3 E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep3_Slot2-5_1_3521.20176.1335.2 2 3 1 21.11 7.11 15.52 14.01 94.3 10 1 1 92100.0 Hydrogen 11 (T) 11 TAVSNLVSRAW (-) 11 AVTSNRKEIW T/A/V|S|N|L/V|S/R|A\W 100.0 11 6 9 5 0 dual b/y bias 69.697708 0.917297 602.3249 1203.648 -0.0053 -4.4 0.1999999999999993 9.41 2415.9 nuORFdb_human ENST00000343677.3_1_6:26056391-26056457:- ENST00000343677.3_1_6:26056391-26056457:- HIST1H1C Out-of-Frame Out-of-Frame GN=HIST1H1C Out-of-Frame E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep3_Slot2-5_1_3521 40mio-rep4 E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep4_Slot2-5_1_3522.20187.1333.2 2 4 1 18.83 4.78 16.46 14.04 89.2 9 1 1 89600.0 Hydrogen 11 (T) 11 TAVSNLVSRAW (-) 11 KETNLVMLQQ T A/V/S|N|L/V|S|R|A\W 81.8 9 6 8 5 0 dual b/y bias 69.71859 0.918448 602.3249 1203.648 -0.0053 -4.4 0.2999999999999998 9.41 2415.9 nuORFdb_human ENST00000343677.3_1_6:26056391-26056457:- ENST00000343677.3_1_6:26056391-26056457:- HIST1H1C Out-of-Frame Out-of-Frame GN=HIST1H1C Out-of-Frame E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep4_Slot2-5_1_3522 5mio-rep1 E_20221201_NO30_400nL_HLAc1_5e6_directIP_titration_rep1_Slot1-37_1_3502.20236.1353.2 2 1 1 13.25 4.9 10.09 8.35 81.0 8 1 1 3200.0 Hydrogen 11 (T) 11 TAVSNLVSRAW (-) 11 KETNLVMLQQ T A/V/S N/L/V|S/R\A\W 63.6 7 3 6 1 0 y-ion bias 69.893875 0.905562 602.3219 1203.648 -0.0113 -9.4 -2.4000000000000004 9.41 2415.9 nuORFdb_human ENST00000343677.3_1_6:26056391-26056457:- ENST00000343677.3_1_6:26056391-26056457:- HIST1H1C Out-of-Frame Out-of-Frame GN=HIST1H1C Out-of-Frame E_20221201_NO30_400nL_HLAc1_5e6_directIP_titration_rep1_Slot1-37_1_3502 SCP E_20221201_NO30_400nL_HLAc1_4e7_PDAC_Slot2-6_1_3524.17873.396.1 1 1 1 10.83 -1.23 11.93 12.06 78.9 7 0 0 8970.0 Hydrogen 17 (K) 9 TCFGTATEF (I) 9 FETTmKCT T C/F|G\T\A\T\E\F 77.8 7 6 2 1 0 b-ion bias Carbamidomethylation C:Carbamidomethylation, 61.746086 1.487695 1033.4276 1033.429 0.0005 0.5 2.9 4.0 4582.4 nuORFdb_human ENST00000569681.5_2_16:50776726-50783580:+ ENST00000569681.5_2_16:50776726-50783580:+|ENST00000566679.6_1_16:50776726-50783580:+ CYLD 5' uORF 5' uORF GN=CYLD 5' uORF E_20221201_NO30_400nL_HLAc1_4e7_PDAC_Slot2-6_1_3524 SCP E_20221201_NO30_400nL_HLAc1_4e7_PDAC_Slot2-6_1_3524.15411.1431.2 2 1 1 11.99 8.53 11.99 70.6 7 0 0 10900.0 Hydrogen 57 (D) 9 TCVGVHVLY (L) 9 T/C|V|G V/H\V|L\Y 77.8 7 5 5 3 0 dual b/y bias Carbamidomethylation C:Carbamidomethylation, 53.241806 0.856846 524.2655 1047.529 -0.0065 -6.2 -3.8000000000000003 6.4 8414.2 nuORFdb_human ENST00000608796.5_1_9:131051046-131051265:+ ENST00000608796.5_1_9:131051046-131051265:+ SWI5 3' dORF 3' dORF GN=SWI5 3' dORF E_20221201_NO30_400nL_HLAc1_4e7_PDAC_Slot2-6_1_3524 10mio-rep1 E_20221201_NO30_400nL_HLAc1_1e7_directIP_titration_rep1_Slot2-3_1_3512.13974.535.1 1 1 1 11.6 7.71 6.71 3.89 79.4 6 0 0 6760.0 Hydrogen 33 (G) 9 TEATVDTSF (-) 9 ESVSnVTSF T E A|T|V|D\T\S\F 66.7 6 6 3 3 0 dual b/y bias 48.275063 1.405088 970.431 970.436 -0.0054 -5.5 2.2 3.66 4258.6 nuORFdb_human ENST00000370823.7_1_20:60770876-60771002:+ ENST00000370823.7_1_20:60770876-60771002:+ MTG2 Out-of-Frame Out-of-Frame GN=MTG2 Out-of-Frame E_20221201_NO30_400nL_HLAc1_1e7_directIP_titration_rep1_Slot2-3_1_3512 10mio-rep2 E_20221201_NO30_400nL_HLAc1_1e7_directIP_titration_rep2_Slot2-3_1_3513.13937.531.1 1 2 1 15.82 7.39 9.33 8.43 85.4 7 0 0 14000.0 Hydrogen 33 (G) 9 TEATVDTSF (-) 9 ESVSnVTSF T E|A|T|V|D|T\S\F 77.8 7 7 5 5 0 dual b/y bias 48.146709 1.407313 970.4293 970.436 -0.0074 -7.6 -0.8999999999999995 3.66 4258.6 nuORFdb_human ENST00000370823.7_1_20:60770876-60771002:+ ENST00000370823.7_1_20:60770876-60771002:+ MTG2 Out-of-Frame Out-of-Frame GN=MTG2 Out-of-Frame E_20221201_NO30_400nL_HLAc1_1e7_directIP_titration_rep2_Slot2-3_1_3513 10mio-rep3 E_20221201_NO30_400nL_HLAc1_1e7_directIP_titration_rep3_Slot2-3_1_3514.13888.537.1 1 2 1 12.44 6.22 6.97 6.22 81.8 7 0 0 22600.0 Hydrogen 33 (G) 9 TEATVDTSF (-) 9 ESVSnVTSF T E/A|T|V|D\T\S\F 77.8 7 6 4 3 0 dual b/y bias 47.980461 1.403647 970.4258 970.436 -0.0104 -10.7 -4.3999999999999995 3.66 4258.6 nuORFdb_human ENST00000370823.7_1_20:60770876-60771002:+ ENST00000370823.7_1_20:60770876-60771002:+ MTG2 Out-of-Frame Out-of-Frame GN=MTG2 Out-of-Frame E_20221201_NO30_400nL_HLAc1_1e7_directIP_titration_rep3_Slot2-3_1_3514 20mio-rep2 E_20221201_NO30_400nL_HLAc1_2e7_directIP_titration_rep3_Slot2-4_1_3518.13973.526.1 1 2 1 14.5 6.82 8.68 7.68 84.8 7 0 0 11800.0 Hydrogen 33 (G) 9 TEATVDTSF (-) 9 ESVSnVTSF T E/A|T|V|D|T\S\F 77.8 7 6 5 4 0 dual b/y bias 48.263168 1.410719 970.4303 970.436 -0.0064 -6.6 -0.2999999999999998 3.66 4258.6 nuORFdb_human ENST00000370823.7_1_20:60770876-60771002:+ ENST00000370823.7_1_20:60770876-60771002:+ MTG2 Out-of-Frame Out-of-Frame GN=MTG2 Out-of-Frame E_20221201_NO30_400nL_HLAc1_2e7_directIP_titration_rep3_Slot2-4_1_3518 20mio-rep3 E_20221201_NO30_400nL_HLAc1_2e7_directIP_titration_rep4_Slot2-4_1_3519.13895.524.1 1 2 1 14.08 7.27 8.53 6.81 83.6 7 0 0 33800.0 Hydrogen 33 (G) 9 TEATVDTSF (-) 9 ESVSnVTSF T E/A|T|V\D|T\S\F 77.8 7 6 4 3 0 dual b/y bias 47.995537 1.411612 970.4305 970.436 -0.0064 -6.6 -0.7999999999999998 3.66 4258.6 nuORFdb_human ENST00000370823.7_1_20:60770876-60771002:+ ENST00000370823.7_1_20:60770876-60771002:+ MTG2 Out-of-Frame Out-of-Frame GN=MTG2 Out-of-Frame E_20221201_NO30_400nL_HLAc1_2e7_directIP_titration_rep4_Slot2-4_1_3519 40mio-rep1 E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep1_Slot1-04_1_3496.14181.502.1 1 2 1 13.95 8.05 7.46 5.9 84.6 7 0 0 42200.0 Hydrogen 33 (G) 9 TEATVDTSF (-) 9 ESVSnVTSF T E/A|T|V|D\T\S\F 77.8 7 6 4 3 0 dual b/y bias 48.98555 1.424863 970.4339 970.436 -0.0024 -2.5 0.7000000000000002 3.66 4258.6 nuORFdb_human ENST00000370823.7_1_20:60770876-60771002:+ ENST00000370823.7_1_20:60770876-60771002:+ MTG2 Out-of-Frame Out-of-Frame GN=MTG2 Out-of-Frame E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep1_Slot1-04_1_3496 40mio-rep2 E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep2_Slot2-5_1_3520.13873.520.1 1 3 1 13.79 7.43 9.07 6.35 83.4 7 0 0 118000.0 Hydrogen 33 (G) 9 TEATVDTSF (-) 9 ESVSnVTSF T E/A|T|V|D|T\S\F 77.8 7 6 5 4 0 dual b/y bias 47.923271 1.414051 970.4327 970.436 -0.0034 -3.5 1.4000000000000004 3.66 4258.6 nuORFdb_human ENST00000370823.7_1_20:60770876-60771002:+ ENST00000370823.7_1_20:60770876-60771002:+ MTG2 Out-of-Frame Out-of-Frame GN=MTG2 Out-of-Frame E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep2_Slot2-5_1_3520 40mio-rep3 E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep3_Slot2-5_1_3521.13842.520.1 1 2 1 13.75 8.6 5.95 5.15 85.1 7 0 0 42300.0 Hydrogen 33 (G) 9 TEATVDTSF (-) 9 ESVSnVTSF T E/A|T|V\D\T\S\F 77.8 7 6 3 2 0 b-ion bias 47.820015 1.413879 970.4344 970.436 -0.0024 -2.5 2.0999999999999996 3.66 4258.6 nuORFdb_human ENST00000370823.7_1_20:60770876-60771002:+ ENST00000370823.7_1_20:60770876-60771002:+ MTG2 Out-of-Frame Out-of-Frame GN=MTG2 Out-of-Frame E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep3_Slot2-5_1_3521 40mio-rep4 E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep4_Slot2-5_1_3522.13862.519.1 1 3 1 14.47 7.38 9.52 7.09 84.5 7 0 0 114000.0 Hydrogen 33 (G) 9 TEATVDTSF (-) 9 ESVSnVTSF T E/A|T|V|D\T\S\F 77.8 7 6 4 3 0 dual b/y bias 47.872448 1.414726 970.4331 970.436 -0.0034 -3.5 1.2000000000000002 3.66 4258.6 nuORFdb_human ENST00000370823.7_1_20:60770876-60771002:+ ENST00000370823.7_1_20:60770876-60771002:+ MTG2 Out-of-Frame Out-of-Frame GN=MTG2 Out-of-Frame E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep4_Slot2-5_1_3522 5mio-rep1 E_20221201_NO30_400nL_HLAc1_5e6_directIP_titration_rep1_Slot1-37_1_3502.14217.524.1 1 1 1 11.99 6.94 7.3 5.05 78.8 7 0 0 5410.0 Hydrogen 33 (G) 9 TEATVDTSF (-) 9 ESVSnVTSF T E/A/T|V|D|T\S\F 77.8 7 5 5 3 0 dual b/y bias 49.107544 1.411621 970.4308 970.436 -0.0054 -5.5 1.5 3.66 4258.6 nuORFdb_human ENST00000370823.7_1_20:60770876-60771002:+ ENST00000370823.7_1_20:60770876-60771002:+ MTG2 Out-of-Frame Out-of-Frame GN=MTG2 Out-of-Frame E_20221201_NO30_400nL_HLAc1_5e6_directIP_titration_rep1_Slot1-37_1_3502 5mio-rep2 E_20221201_NO30_400nL_HLAc1_5e6_directIP_titration_rep2_Slot2-2_1_3510.14157.534.1 1 2 1 13.12 5.84 7.43 7.28 83.4 7 0 0 8240.0 Hydrogen 33 (G) 9 TEATVDTSF (-) 9 ESVSnVTSF T E/A|T|V|D\T\S\F 77.8 7 6 4 3 0 dual b/y bias 48.898373 1.405747 970.431 970.436 -0.0054 -5.5 3.1999999999999993 3.66 4258.6 nuORFdb_human ENST00000370823.7_1_20:60770876-60771002:+ ENST00000370823.7_1_20:60770876-60771002:+ MTG2 Out-of-Frame Out-of-Frame GN=MTG2 Out-of-Frame E_20221201_NO30_400nL_HLAc1_5e6_directIP_titration_rep2_Slot2-2_1_3510 5mio-rep3 E_20221201_NO30_400nL_HLAc1_5e6_directIP_titration_rep3_Slot2-2_1_3511.14157.531.1 1 1 1 14.96 7.57 8.27 7.38 86.7 7 0 0 2300.0 Hydrogen 33 (G) 9 TEATVDTSF (-) 9 ESVSnVTSF T E/A|T|V|D\T\S\F 77.8 7 6 4 3 0 dual b/y bias 48.903549 1.407722 970.4264 970.436 -0.0104 -10.7 -2.5 3.66 4258.6 nuORFdb_human ENST00000370823.7_1_20:60770876-60771002:+ ENST00000370823.7_1_20:60770876-60771002:+ MTG2 Out-of-Frame Out-of-Frame GN=MTG2 Out-of-Frame E_20221201_NO30_400nL_HLAc1_5e6_directIP_titration_rep3_Slot2-2_1_3511 40mio-rep1 E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep1_Slot1-04_1_3496.28895.286.1 1 1 1 8.71 3.63 3.94 5.08 64.6 6 0 0 3270.0 Hydrogen 46 (P) 9 TEIELTSFF (S) 9 QPSSTLDIPE T E I\E|L|T\S\F\F 66.7 6 6 2 2 0 b-ion bias 99.823227 1.552685 1086.5348 1086.535 -0.0054 -4.9 -1.7000000000000002 3.79 6572.5 nuORFdb_human ENST00000313146.10_1_1:156697597-156697768:- ENST00000313146.10_1_1:156697597-156697768:- ISG20L2 5' uORF 5' uORF GN=ISG20L2 5' uORF E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep1_Slot1-04_1_3496 40mio-rep1 E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep1_Slot1-04_1_3496.15396.1601.3 3 4 1 10.12 5.86 4.02 4.26 77.9 7 2 2 28600.0 Hydrogen 38 (K) 10 TELKSFPREW (H) 10 TFRPGFPSSSIP T/E/L/K/S\F|P\R E W 70.0 7 3 5 1 0 unbiased 53.18259 0.749971 431.557 1292.663 -0.0069 -5.4 -2.2 5.81 5597.1 nuORFdb_human ENST00000555474.5_1_14:67819743-67826513:- ENST00000555474.5_1_14:67819743-67826513:-|ENST00000553687.1_2_14:67820623-67826513:- ATP6V1D 5' Overlap uORF 5' Overlap uORF GN=ATP6V1D 5' Overlap uORF E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep1_Slot1-04_1_3496 40mio-rep2 E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep2_Slot2-5_1_3520.12327.1396.2 2 1 1 14.69 5.09 11.52 9.6 81.4 7 1 1 7960.0 Hydrogen 199 (H) 10 TEVAEPLKTY (K) 10 LnTAEALFLSA T E|V|A|E|P L|K|T\Y 60.0 6 7 6 6 0 dual b/y bias 42.584534 0.878859 575.8005 1150.599 -0.0043 -3.7 1.2000000000000002 4.53 23561.2 nuORFdb_human ENST00000484415.6_1_X:155251663-155252984:+ ENST00000484415.6_1_X:155251663-155252984:+|ENST00000482170.6_1_X:155252010-155252984:+|ENST00000461007.6_1_X:155252382-155252984:+|ENST00000538033.2_1_2:114353729-114354265:+ WASH6P Pseudogene Pseudogene GN=WASH6P Pseudogene E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep2_Slot2-5_1_3520 Eva-50mio-3fr AL20210827_EV_HLA_PDAC_PANFRO413_2D_50e6_W632_classI_fxn2.34788.34886.2 2 1 1 13.57 7.61 6.9 5.95 79.0 6 0 0 54000000.0 Hydrogen 9 (S) 9 TFQVESHFF (K) 9 TFQDFSHIF T F|Q|V/E S/H-F/F 55.6 5 2 5 2 1 y-ion bias 0.995 87.1 63.87 59.89 0.0 571.269 1141.531 -0.0007 -0.6 -0.8999999999999999 5.21 3384.7 nuORFdb_human ENST00000382040.3_1_2:7018268-7023576:+ ENST00000382040.3_1_2:7018268-7023576:+ RSAD2 Out-of-Frame Out-of-Frame GN=RSAD2 Out-of-Frame AL20210827_EV_HLA_PDAC_PANFRO413_2D_50e6_W632_classI_fxn2 Exploris_FAIMS VH20221207_MKP_FAIMS2022E_HLAc1_4e7_PDAC.56892.56892.2 2 3 1 10.38 5.57 5.51 4.81 64.6 6 0 0 176000000.0 Hydrogen 9 (S) 9 TFQVESHFF (K) 9 TFQDFSHIF T F\Q/V/E S/H-F/F 55.6 5 1 4 0 1 y-ion bias 0.973 49.9 99.08 43.41 -50.0 571.2686 1141.531 -0.0013 -1.1 1.5 5.21 3384.7 nuORFdb_human ENST00000382040.3_1_2:7018268-7023576:+ ENST00000382040.3_1_2:7018268-7023576:+ RSAD2 Out-of-Frame Out-of-Frame GN=RSAD2 Out-of-Frame VH20221207_MKP_FAIMS2022E_HLAc1_4e7_PDAC Eva-50mio-3fr AL20210827_EV_HLA_PDAC_PANFRO413_2D_50e6_W632_classI_fxn3.37841.37841.2 2 1 1 11.22 4.9 5.37 6.31 73.8 5 1 1 49900000.0 Hydrogen 82 (S) 9 TIFKFVEAY (T) 9 TLVKSmLFY T I|F K F\V-E/A/Y 44.4 4 2 3 1 1 unbiased 0.997 94.2 68.6 100.0 0.0 559.3021 1117.593 0.004 3.6 3.1 5.66 21306.4 nuORFdb_human ENST00000431677.1_1_X:100673784-100702742:+ ENST00000431677.1_1_X:100673784-100702742:+|ENST00000433011.5_1_X:100699118-100701032:+ ARMCX4 ncRNA Processed Transcript lncRNA GN=ARMCX4 ncRNA Processed Transcript AL20210827_EV_HLA_PDAC_PANFRO413_2D_50e6_W632_classI_fxn3 40mio-rep1 E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep1_Slot1-04_1_3496.13663.1364.2 2 1 1 15.22 11.37 3.26 3.85 90.9 8 2 2 8460.0 Hydrogen 2 (L) 9 TIKVRNIPL (-) 9 TLKQLIQPI T/I/K/V\R\N/I|P\L 100.0 9 4 5 1 0 unbiased 47.196331 0.899035 527.342 1053.678 -0.0012 -1.1 2.1 11.0 1166.5 nuORFdb_human ENST00000619445.1_1_X:118979238-118985469:- ENST00000619445.1_1_X:118979238-118985469:- UPF3B Out-of-Frame Out-of-Frame GN=UPF3B Out-of-Frame E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep1_Slot1-04_1_3496 10mio-rep1 E_20221201_NO30_400nL_HLAc1_1e7_directIP_titration_rep1_Slot2-3_1_3512.20593.1493.2 2 1 1 9.77 7.7 1.52 2.08 77.4 5 1 1 3220.0 Hydrogen 48 (G) 8 TIRDPLLL (L) 8 TRDLPIIL T I R|D\P\L|L\L 62.5 5 5 2 2 0 b-ion bias 71.135414 0.817721 470.7924 940.583 -0.0059 -6.2 1.5 5.5 6988.0 nuORFdb_human ENST00000459931.1_1_1:36023779-36023974:+ ENST00000459931.1_1_1:36023779-36023974:+ NCDN 5' Overlap uORF 5' Overlap uORF GN=NCDN 5' Overlap uORF E_20221201_NO30_400nL_HLAc1_1e7_directIP_titration_rep1_Slot2-3_1_3512 20mio-rep1 E_20221201_NO30_400nL_HLAc1_2e7_directIP_titration_rep1_Slot2-4_1_3515.20525.1484.2 2 1 1 14.12 12.09 2.03 2.03 86.2 6 1 1 4630.0 Hydrogen 48 (G) 8 TIRDPLLL (L) 8 LKEDPLIL T I/R|D|P\L|L\L 75.0 6 5 4 3 0 dual b/y bias 70.880997 0.823328 470.793 940.583 -0.0039 -4.1 2.1000000000000005 5.5 6988.0 nuORFdb_human ENST00000459931.1_1_1:36023779-36023974:+ ENST00000459931.1_1_1:36023779-36023974:+ NCDN 5' Overlap uORF 5' Overlap uORF GN=NCDN 5' Overlap uORF E_20221201_NO30_400nL_HLAc1_2e7_directIP_titration_rep1_Slot2-4_1_3515 40mio-rep1 E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep1_Slot1-04_1_3496.20742.1458.2 2 4 1 10.91 9.34 1.57 1.57 79.6 5 1 1 19300.0 Hydrogen 48 (G) 8 TIRDPLLL (L) 8 TRDLPIIL T I/R D|P\L\L\L 50.0 4 4 2 1 0 b-ion bias 71.653435 0.839732 470.7937 940.583 -0.0019 -2.0 1.2000000000000002 5.5 6988.0 nuORFdb_human ENST00000459931.1_1_1:36023779-36023974:+ ENST00000459931.1_1_1:36023779-36023974:+ NCDN 5' Overlap uORF 5' Overlap uORF GN=NCDN 5' Overlap uORF E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep1_Slot1-04_1_3496 40mio-rep2 E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep2_Slot2-5_1_3520.20678.1468.2 2 1 1 12.07 10.55 2.02 1.52 85.0 7 1 1 8700.0 Hydrogen 48 (G) 8 TIRDPLLL (L) 8 LTRDLPIL T/I/R\D\P\L|L\L 100.0 8 5 3 1 0 unbiased 71.434921 0.833633 470.792 940.583 -0.0059 -6.2 -1.2999999999999998 5.5 6988.0 nuORFdb_human ENST00000459931.1_1_1:36023779-36023974:+ ENST00000459931.1_1_1:36023779-36023974:+ NCDN 5' Overlap uORF 5' Overlap uORF GN=NCDN 5' Overlap uORF E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep2_Slot2-5_1_3520 40mio-rep3 E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep3_Slot2-5_1_3521.20615.1470.2 2 2 1 12.22 10.18 1.0 2.05 89.2 6 1 1 11300.0 Hydrogen 48 (G) 8 TIRDPLLL (L) 8 TRDLPIIL T I/R\D\P\L|L\L 75.0 6 5 2 1 0 b-ion bias 71.21357 0.832265 470.7925 940.583 -0.0039 -4.1 0.5 5.5 6988.0 nuORFdb_human ENST00000459931.1_1_1:36023779-36023974:+ ENST00000459931.1_1_1:36023779-36023974:+ NCDN 5' Overlap uORF 5' Overlap uORF GN=NCDN 5' Overlap uORF E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep3_Slot2-5_1_3521 40mio-rep4 E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep4_Slot2-5_1_3522.20503.1476.2 2 3 1 10.73 9.7 1.0 1.03 89.1 5 1 1 19200.0 Hydrogen 48 (G) 8 TIRDPLLL (L) 8 LTDVGIPLL T I/R D\P\L|L\L 50.0 4 4 2 1 0 b-ion bias 70.810417 0.828916 470.7937 940.583 -0.0019 -2.0 2.7 5.5 6988.0 nuORFdb_human ENST00000459931.1_1_1:36023779-36023974:+ ENST00000459931.1_1_1:36023779-36023974:+ NCDN 5' Overlap uORF 5' Overlap uORF GN=NCDN 5' Overlap uORF E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep4_Slot2-5_1_3522 40mio-rep2 E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep2_Slot2-5_1_3520.24779.483.1 1 2 1 9.35 7.7 2.05 1.64 78.3 6 0 0 49700.0 Hydrogen 52 (R) 9 TLAVSVTVL (K) 9 ALTSVVVTL T L A\V\S\V\T-V\L 66.7 6 5 0 0 1 b-ion bias 85.598 1.436057 902.5539 902.556 -0.0017 -1.9 3.0000000000000004 5.18 7488.5 nuORFdb_human T053523_11:3449534-3455475:+ T053523_11:3449534-3455475:+ lincRNA lncRNA GN=nan lincRNA E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep2_Slot2-5_1_3520 20mio-rep1 E_20221201_NO30_400nL_HLAc1_2e7_directIP_titration_rep1_Slot2-4_1_3515.18632.513.1 1 3 1 8.84 2.47 2.11 6.37 76.2 6 0 0 6520.0 Hydrogen 9 (V) 9 TLDEPPLPA (G) 9 RFAFTPVn T L D|E/P\P\L\P-A 66.7 6 4 2 1 1 b-ion bias 64.346481 1.417989 952.4905 952.499 -0.0086 -9.0 -2.8 3.66 8056.1 smORF_v4|nuORFdb_human LINC00460_13:107029289-107029628:+ LINC00460_13:107029289-107029628:+|ENST00000439790.5_1_13:107029264-107029625:+|ENST00000435024.1_1_13:107029264-107030327:+ LINC00460 lncRNA 13:107029289-107029628:+ 13 LINC00460 lincRNA lncRNA GN=LINC00460 E_20221201_NO30_400nL_HLAc1_2e7_directIP_titration_rep1_Slot2-4_1_3515 40mio-rep1 E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep1_Slot1-04_1_3496.18893.488.1 1 2 1 9.5 6.92 2.21 2.59 82.6 6 0 0 6400.0 Hydrogen 9 (V) 9 TLDEPPLPA (G) 9 DPETLPLPA T L D/E/P\P\L\P\A 66.7 6 4 2 0 0 b-ion bias 65.265877 1.432878 952.4938 952.499 -0.0046 -4.8 -1.5999999999999996 3.66 8056.1 smORF_v4|nuORFdb_human LINC00460_13:107029289-107029628:+ LINC00460_13:107029289-107029628:+|ENST00000439790.5_1_13:107029264-107029625:+|ENST00000435024.1_1_13:107029264-107030327:+ LINC00460 lncRNA 13:107029289-107029628:+ 13 LINC00460 lincRNA lncRNA GN=LINC00460 E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep1_Slot1-04_1_3496 40mio-rep4 E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep4_Slot2-5_1_3522.18610.504.1 1 2 1 7.68 2.67 1.45 5.01 68.8 6 0 0 6560.0 Hydrogen 9 (V) 9 TLDEPPLPA (G) 9 METPPLPPA T L D/E/P\P\L\P-A 66.7 6 3 2 0 1 unbiased 64.270218 1.423459 952.4953 952.499 -0.0036 -3.8 0.9000000000000004 3.66 8056.1 smORF_v4|nuORFdb_human LINC00460_13:107029289-107029628:+ LINC00460_13:107029289-107029628:+|ENST00000439790.5_1_13:107029264-107029625:+|ENST00000435024.1_1_13:107029264-107030327:+ LINC00460 lncRNA 13:107029289-107029628:+ 13 LINC00460 lincRNA lncRNA GN=LINC00460 E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep4_Slot2-5_1_3522 20mio-rep3 E_20221201_NO30_400nL_HLAc1_2e7_directIP_titration_rep4_Slot2-4_1_3519.21745.432.1 1 1 1 8.16 5.27 2.7 2.9 70.9 6 0 0 3020.0 Hydrogen 30 (M) 9 TLDVQTVVV (F) 9 LVnTAGTVVV T L D\V/Q\T\V\V\V 66.7 6 5 1 0 0 b-ion bias 75.10173 1.466324 973.55 973.556 -0.0064 -6.6 -0.7999999999999998 3.8 6693.9 smORF_v4|nuORFdb_human ARHGEF9_X:62974365-62974544:- ARHGEF9_X:62974365-62974544:-|ENST00000374872.4_2_X:62974367-62974952:- ARHGEF9 uORF X:62974365-62974544:- X ARHGEF9 5' uORF 5' uORF GN=ARHGEF9 E_20221201_NO30_400nL_HLAc1_2e7_directIP_titration_rep4_Slot2-4_1_3519 40mio-rep2 E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep2_Slot2-5_1_3520.29674.381.1 1 1 1 9.29 7.05 2.24 2.24 78.1 7 0 0 3450.0 Hydrogen 40 (C) 9 TLLILPVPV (-) 9 LISPLLLPV T L|L|I|L|P\V|P-V 77.8 7 6 5 5 1 dual b/y bias 102.509384 1.496644 964.6439 964.644 -0.0001 -0.1 4.800000000000001 5.18 5479.3 nuORFdb_human ENST00000317881.8_1_X:118603789-118603936:+ ENST00000317881.8_1_X:118603789-118603936:+ SLC25A5 Out-of-Frame Out-of-Frame GN=SLC25A5 Out-of-Frame E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep2_Slot2-5_1_3520 40mio-rep4 E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep4_Slot2-5_1_3522.19817.561.1 1 1 1 9.16 6.45 2.72 2.72 64.3 6 0 0 6100.0 Hydrogen 3 (S) 9 TLLISSSGV (-) 9 LTILSSKD T L/L|I|S\S|S G\V 55.6 5 5 4 3 0 dual b/y bias 68.441254 1.389404 876.5 876.504 -0.0037 -4.2 0.5 5.18 1094.3 nuORFdb_human ENST00000528489.1_1_11:58345486-58345519:- ENST00000528489.1_1_11:58345486-58345519:- LPXN 5' Overlap uORF 5' Overlap uORF GN=LPXN 5' Overlap uORF E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep4_Slot2-5_1_3522 40mio-rep4 E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep4_Slot2-5_1_3522.28595.1458.2 2 1 1 12.22 10.52 1.08 1.7 86.8 5 0 0 4010.0 Hydrogen 54 (T) 9 TLTSLNILL (L) 9 LSTLNITLL T L/T S L\N\I|L\L 44.4 4 4 2 1 0 b-ion bias 98.765602 0.839701 494.3059 987.609 -0.0038 -3.8 0.9000000000000004 5.18 7163.2 nuORFdb_human ENST00000373840.8_1_9:123954490-123964046:- ENST00000373840.8_1_9:123954490-123964046:- RAB14 5' Overlap uORF 5' Overlap uORF GN=RAB14 5' Overlap uORF E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep4_Slot2-5_1_3522 40mio-rep1 E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep1_Slot1-04_1_3496.24128.396.1 1 1 1 8.22 1.84 8.22 6.38 62.1 6 0 0 5250.0 Hydrogen 27 (N) 9 TLYSVGVTL (K) 9 TLYDKLSI T L/Y|S|V G\V\T-L 55.6 5 4 3 2 1 unbiased 83.351105 1.487579 952.5344 952.535 -0.001 -1.0 2.2 5.19 9113.6 nuORFdb_human ENST00000589878.5_1_19:34663659-34686187:+ ENST00000589878.5_1_19:34663659-34686187:+ LSM14A 3' dORF 3' dORF GN=LSM14A 3' dORF E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep1_Slot1-04_1_3496 40mio-rep2 E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep2_Slot2-5_1_3520.23914.420.1 1 1 1 7.11 1.23 5.44 5.88 66.8 6 0 0 4280.0 Hydrogen 27 (N) 9 TLYSVGVTL (K) 9 TYLVSGSLI T L/Y|S/V G\V\T-L 55.6 5 3 3 1 1 unbiased 82.610573 1.473607 952.532 952.535 -0.003 -3.1 1.8000000000000003 5.19 9113.6 nuORFdb_human ENST00000589878.5_1_19:34663659-34686187:+ ENST00000589878.5_1_19:34663659-34686187:+ LSM14A 3' dORF 3' dORF GN=LSM14A 3' dORF E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep2_Slot2-5_1_3520 Eva-50mio-3fr AL20210827_EV_HLA_PDAC_PANFRO413_2D_50e6_W632_classI_fxn1.18243.18243.2 2 1 1 11.05 8.39 6.17 2.66 66.8 5 1 1 25100000.0 Hydrogen 25 (V) 9 TPAERGLVY (H) 9 TASHSIKVY T/P|A/E R G L|V|Y 55.6 5 3 5 3 0 dual b/y bias 0.995 84.0 34.67 100.0 0.0 503.2719 1005.536 0.0002 0.2 0.1 5.66 4110.6 nuORFdb_human ENST00000357085.4_2_6:25963311-25963422:+ ENST00000357085.4_2_6:25963311-25963422:+ TRIM38 5' uORF 5' uORF GN=TRIM38 5' uORF AL20210827_EV_HLA_PDAC_PANFRO413_2D_50e6_W632_classI_fxn1 Exploris AL20221201_MKP_PDAC_01.22946.22946.2 2 1 1 10.83 7.22 5.98 3.61 72.5 5 1 1 123000000.0 Hydrogen 25 (V) 9 TPAERGLVY (H) 9 TASHSIKVY T/P|A/E R G L\V|Y 55.6 5 3 4 2 0 unbiased 0.858 63.5 38.92 26.96 0.0 503.2715 1005.536 -0.0007 -0.7 -1.5 5.66 4110.6 nuORFdb_human ENST00000357085.4_2_6:25963311-25963422:+ ENST00000357085.4_2_6:25963311-25963422:+ TRIM38 5' uORF 5' uORF GN=TRIM38 5' uORF AL20221201_MKP_PDAC_01 SCP E_20221201_NO30_400nL_HLAc1_4e7_PDAC_Slot2-6_1_3524.10159.1471.2 2 1 1 14.41 9.8 6.19 4.6 86.5 6 1 1 8820.0 Hydrogen 25 (V) 9 TPAERGLVY (H) 9 TASHSIKVY T/P/A/E R G\L|V\Y 66.7 6 3 4 1 0 unbiased 35.097519 0.832053 503.2696 1005.536 -0.0036 -3.6 -1.2000000000000002 5.66 4110.6 nuORFdb_human ENST00000357085.4_2_6:25963311-25963422:+ ENST00000357085.4_2_6:25963311-25963422:+ TRIM38 5' uORF 5' uORF GN=TRIM38 5' uORF E_20221201_NO30_400nL_HLAc1_4e7_PDAC_Slot2-6_1_3524 Exploris_FAIMS VH20221207_MKP_FAIMS2022E_HLAc1_4e7_PDAC.28397.28397.2 2 2 1 13.44 6.7 9.0 6.74 76.7 7 1 1 138000000.0 Hydrogen 25 (V) 9 TPAERGLVY (H) 9 TASHSIKVY T/P|A/E/R G\L|V|Y 77.8 7 4 6 3 0 dual b/y bias 0.999 98.6 56.42 12.09 -70.0 503.2704 1005.536 -0.0028 -2.8 -0.19999999999999973 5.66 4110.6 nuORFdb_human ENST00000357085.4_2_6:25963311-25963422:+ ENST00000357085.4_2_6:25963311-25963422:+ TRIM38 5' uORF 5' uORF GN=TRIM38 5' uORF VH20221207_MKP_FAIMS2022E_HLAc1_4e7_PDAC SCP E_20221201_NO30_400nL_HLAc1_4e7_PDAC_Slot2-6_1_3524.12442.638.1 1 1 1 12.08 8.88 3.02 3.2 80.7 7 0 0 3210.0 Hydrogen 177 (C) 9 TPAPPASTL (A) 9 TPATGPPTL T/P/A/P|P A\S\T\L 77.8 7 4 4 1 0 unbiased 42.982849 1.34338 854.4565 854.462 -0.0048 -5.6 -3.1999999999999997 5.18 47329.4 nuORFdb_human ENST00000620918.4_1_19:36257819-36259444:+ ENST00000620918.4_1_19:36257819-36259444:+ PROSER3 3' Overlap dORF 3' Overlap dORF GN=PROSER3 3' Overlap dORF E_20221201_NO30_400nL_HLAc1_4e7_PDAC_Slot2-6_1_3524 Exploris AL20221201_MKP_PDAC_01.24096.24096.2 2 2 1 12.1 6.7 4.68 5.41 75.0 6 0 1 121000000.0 Hydrogen 229 (R) 9 TPAPRPAVL (V) 9 TPRPALGPL T/P|A|P R P-A\V|L 66.7 6 4 4 3 1 dual b/y bias 0.999 86.9 39.68 100.0 0.0 461.2802 921.552 0.0015 1.6 0.8 9.41 29890.7 smORF_v4|nuORFdb_human SRCAP_16:30709299-30715403:+ SRCAP_16:30709299-30715403:+|ENST00000411466.6_3_16:30709553-30715400:+|ENST00000262518.8_1_16:30710550-30715400:+ SRCAP uORF 16:30709299-30715403:+ 16 SRCAP 5' Overlap uORF 5' Overlap uORF GN=SRCAP AL20221201_MKP_PDAC_01 SCP E_20221201_NO30_400nL_HLAc1_4e7_PDAC_Slot2-6_1_3524.10661.1527.2 2 3 1 14.13 9.92 5.93 4.22 92.1 7 0 1 70400.0 Hydrogen 229 (R) 9 TPAPRPAVL (V) 9 TPALPAAPAL T/P/A|P/R|P A-V\L 77.8 7 3 5 2 1 unbiased 36.831768 0.796422 461.2777 921.552 -0.0029 -3.2 -0.8000000000000003 9.41 29890.7 smORF_v4|nuORFdb_human SRCAP_16:30709299-30715403:+ SRCAP_16:30709299-30715403:+|ENST00000411466.6_3_16:30709553-30715400:+|ENST00000262518.8_1_16:30710550-30715400:+ SRCAP uORF 16:30709299-30715403:+ 16 SRCAP 5' Overlap uORF 5' Overlap uORF GN=SRCAP E_20221201_NO30_400nL_HLAc1_4e7_PDAC_Slot2-6_1_3524 Exploris_FAIMS VH20221207_MKP_FAIMS2022E_HLAc1_4e7_PDAC.28590.28590.2 2 1 1 17.01 10.26 4.78 6.75 95.3 7 0 1 28600000.0 Hydrogen 229 (R) 9 TPAPRPAVL (V) 9 APTPARPVL T/P|A|P R/P-A|V|L 77.8 7 4 6 4 1 dual b/y bias 1.0 98.1 56.13 18.77 -70.0 461.2781 921.552 -0.0026 -2.9 -0.2999999999999998 9.41 29890.7 smORF_v4|nuORFdb_human SRCAP_16:30709299-30715403:+ SRCAP_16:30709299-30715403:+|ENST00000411466.6_3_16:30709553-30715400:+|ENST00000262518.8_1_16:30710550-30715400:+ SRCAP uORF 16:30709299-30715403:+ 16 SRCAP 5' Overlap uORF 5' Overlap uORF GN=SRCAP VH20221207_MKP_FAIMS2022E_HLAc1_4e7_PDAC SCP E_20221201_NO30_400nL_HLAc1_4e7_PDAC_Slot2-6_1_3524.22028.179.1 1 2 1 10.2 2.21 7.78 7.99 77.5 8 0 0 6760.0 Hydrogen 42 (R) 11 TPCAPAMQVSF (-) 11 SQPQCAGQSVF T P/C/A/P A\M\Q\V\S\F 63.6 7 5 3 0 0 unbiased Carbamidomethylation C:Carbamidomethylation, 76.102707 1.615319 1208.5438 1208.544 -0.0038 -3.2 -0.8000000000000003 5.18 5529.3 nuORFdb_human ENST00000543995.5_1_12:6442265-6443279:- ENST00000543995.5_1_12:6442265-6443279:-|ENST00000366159.8_1_12:6442262-6443328:- TNFRSF1A Out-of-Frame Out-of-Frame GN=TNFRSF1A Out-of-Frame E_20221201_NO30_400nL_HLAc1_4e7_PDAC_Slot2-6_1_3524 Exploris_FAIMS VH20221207_MKP_FAIMS2022E_HLAc1_4e7_PDAC.52035.52035.2 2 1 1 13.97 3.62 11.64 10.35 84.6 8 0 0 5090000.0 Hydrogen 42 (R) 11 TPCAPAMQVSF (-) 11 PRGAEADCVSF T P C\A\P-A-M\Q\V/S/F 72.7 8 4 2 0 2 b-ion bias Carbamidomethylation C:Carbamidomethylation, 0.98 100.0 91.45 120.0 -70.0 604.7743 1208.544 -0.0025 -2.1 0.5 5.18 5529.3 nuORFdb_human ENST00000543995.5_1_12:6442265-6443279:- ENST00000543995.5_1_12:6442265-6443279:-|ENST00000366159.8_1_12:6442262-6443328:- TNFRSF1A Out-of-Frame Out-of-Frame GN=TNFRSF1A Out-of-Frame VH20221207_MKP_FAIMS2022E_HLAc1_4e7_PDAC SCP E_20221201_NO30_400nL_HLAc1_4e7_PDAC_Slot2-6_1_3524.8977.440.1 1 1 1 8.18 1.88 6.11 6.29 74.9 7 0 0 1720.0 Hydrogen 14 (C) 9 TPHEEALSY (G) 9 ANGHSPEHTP T P/H\E\E\A\L\S\Y 77.8 7 6 1 0 0 b-ion bias 31.013264 1.461649 1046.4841 1046.479 0.0011 1.0 3.4 4.5 3994.5 nuORFdb_human ENST00000463441.5_1_7:150025734-150025836:+ ENST00000463441.5_1_7:150025734-150025836:+ LRRC61 ncRNA Processed Transcript lncRNA GN=LRRC61 ncRNA Processed Transcript E_20221201_NO30_400nL_HLAc1_4e7_PDAC_Slot2-6_1_3524 SCP E_20221201_NO30_400nL_HLAc1_4e7_PDAC_Slot2-6_1_3524.29473.459.1 1 1 1 11.4 4.33 7.07 7.07 85.4 5 0 0 11700.0 Hydrogen 25 (G) 8 TPIIPFVM (D) 8 TPLLNKMT T P I|I|P\F\V\M 62.5 5 5 2 2 0 b-ion bias 101.828171 1.45015 917.5134 917.516 -0.0035 -3.8 -1.4 5.18 17668.9 nuORFdb_human ENST00000412609.5_1_19:40170059-40174276:+ ENST00000412609.5_1_19:40170059-40174276:+|ENST00000598164.2_1_19:40172096-40174784:+ LGALS17A Pseudogene Pseudogene GN=LGALS17A Pseudogene E_20221201_NO30_400nL_HLAc1_4e7_PDAC_Slot2-6_1_3524 Exploris_FAIMS VH20221207_MKP_FAIMS2022E_HLAc1_4e7_PDAC.59377.59377.2 2 1 1 10.13 1.9 6.72 8.23 63.2 5 1 1 1910000.0 Hydrogen 21 (L) 10 TPLWEKGLVY (C) 10 AITPLANPFVY T P\L|W E/K G L|V/Y 30.0 3 3 4 2 0 unbiased 0.978 82.3 103.13 120.0 -70.0 603.3308 1205.657 -0.0022 -1.8 0.8 5.66 3901.4 nuORFdb_human ENST00000421904.5_1_2:25194480-25195552:+ ENST00000421904.5_1_2:25194480-25195552:+|ENST00000421842.1_1_2:25195072-25195552:+ DNAJC27-AS1 Antisense lncRNA GN=DNAJC27-AS1 Antisense VH20221207_MKP_FAIMS2022E_HLAc1_4e7_PDAC SCP E_20221201_NO30_400nL_HLAc1_4e7_PDAC_Slot2-6_1_3524.15208.1403.2 2 2 1 12.49 7.96 3.46 4.53 67.3 7 0 1 29300.0 Hydrogen 3 (T) 10 TPRPPPQINL (-) 10 LGRAQTFLNI T/P R|P P/P\Q|I|N\L 60.0 6 5 5 3 0 unbiased 52.540913 0.874564 566.8254 1132.647 -0.0046 -4.1 -1.6999999999999997 9.41 1364.6 nuORFdb_human ENST00000597550.5_1_19:53700488-53703885:+ ENST00000597550.5_1_19:53700488-53703885:+ CTD-2245F17.3 Pseudogene Pseudogene GN=CTD-2245F17.3 Pseudogene E_20221201_NO30_400nL_HLAc1_4e7_PDAC_Slot2-6_1_3524 SCP E_20221201_NO30_400nL_HLAc1_4e7_PDAC_Slot2-6_1_3524.8411.1544.2 2 1 1 9.79 4.89 4.48 4.9 65.6 8 0 1 11400.0 Hydrogen 264 (V) 9 TPTRPGPTL (R) 9 TPRPTGTPL T/P/T/R|P/G\P\T\L 100.0 9 4 5 1 0 unbiased 29.05854 0.785601 470.2643 939.526 -0.0051 -5.4 -3.0000000000000004 9.41 32357.7 nuORFdb_human ENST00000308893.8_1_16:29985169-29989140:+ ENST00000308893.8_1_16:29985169-29989140:+ TAOK2 5' Overlap uORF 5' Overlap uORF GN=TAOK2 5' Overlap uORF E_20221201_NO30_400nL_HLAc1_4e7_PDAC_Slot2-6_1_3524 Exploris_FAIMS VH20221207_MKP_FAIMS2022E_HLAc1_4e7_PDAC.23567.23567.2 2 1 1 12.73 7.87 4.55 4.86 67.2 7 0 1 4890000.0 Hydrogen 264 (V) 9 TPTRPGPTL (R) 9 TPRPTGTPL T/P|T/R|P G|P\T|L 77.8 7 5 6 4 0 dual b/y bias 0.975 60.4 48.73 75.22 -50.0 470.2657 939.526 -0.0017 -1.8 0.8 9.41 32357.7 nuORFdb_human ENST00000308893.8_1_16:29985169-29989140:+ ENST00000308893.8_1_16:29985169-29989140:+ TAOK2 5' Overlap uORF 5' Overlap uORF GN=TAOK2 5' Overlap uORF VH20221207_MKP_FAIMS2022E_HLAc1_4e7_PDAC Exploris AL20221201_MKP_PDAC_01.45720.45851.1 1 4 1 15.48 6.48 7.79 9.0 95.7 7 0 0 459000000.0 Hydrogen 32 (V) 9 TPVAVITDM (K) 9 LVAPTVSME T P\V\A\V\I|T/D-M 77.8 7 5 2 1 1 b-ion bias 0.987 100.0 70.07 100.0 0.0 946.4917 946.491 0.0003 0.3 -0.5 3.8 21191.7 nuORFdb_human ENST00000354911.8_2_10:28822518-28879725:+ ENST00000354911.8_2_10:28822518-28879725:+ WAC Out-of-Frame Out-of-Frame GN=WAC Out-of-Frame AL20221201_MKP_PDAC_01 SCP E_20221201_NO30_400nL_HLAc1_4e7_PDAC_Slot2-6_1_3524.21068.496.1 1 8 1 16.4 10.27 7.41 6.13 95.9 6 0 0 387000.0 Hydrogen 32 (V) 9 TPVAVITDM (K) 9 GPITVTInM T P V|A|V|I|T\D\M 66.7 6 6 4 4 0 dual b/y bias 72.782013 1.428577 946.4894 946.491 -0.0024 -2.5 -0.10000000000000009 3.8 21191.7 nuORFdb_human ENST00000354911.8_2_10:28822518-28879725:+ ENST00000354911.8_2_10:28822518-28879725:+ WAC Out-of-Frame Out-of-Frame GN=WAC Out-of-Frame E_20221201_NO30_400nL_HLAc1_4e7_PDAC_Slot2-6_1_3524 Eva-50mio-3fr AL20210827_EV_HLA_PDAC_PANFRO413_2D_50e6_W632_classI_fxn1.23690.23690.1 1 3 1 16.8 6.64 8.28 10.17 93.2 7 0 0 271000000.0 M40m Hydrogen 32 (V) 9 TPVAVITDm (K) 9 GPITVTInm T P\V\A\V|I|T/D|m 77.8 7 6 4 3 0 dual b/y bias m:Oxidized methionine 0.713 100.0 43.57 100.0 0.0 962.4862 946.491 15.9948 -0.1 -0.2 3.8 21191.7 nuORFdb_human ENST00000354911.8_2_10:28822518-28879725:+ ENST00000354911.8_2_10:28822518-28879725:+ WAC Out-of-Frame Out-of-Frame GN=WAC Out-of-Frame AL20210827_EV_HLA_PDAC_PANFRO413_2D_50e6_W632_classI_fxn1 Exploris AL20221201_MKP_PDAC_01.32219.32219.2 2 2 1 12.24 4.7 6.08 7.54 77.5 7 0 0 384000000.0 M40m Hydrogen 32 (V) 9 TPVAVITDm (K) 9 TVIGLPTnm T P\V\A\V\I|T/D|m 77.8 7 6 3 2 0 b-ion bias m:Oxidized methionine 0.985 76.0 51.23 11.61 0.0 481.7472 946.491 15.9957 0.8 0.0 3.8 21191.7 nuORFdb_human ENST00000354911.8_2_10:28822518-28879725:+ ENST00000354911.8_2_10:28822518-28879725:+ WAC Out-of-Frame Out-of-Frame GN=WAC Out-of-Frame AL20221201_MKP_PDAC_01 SCP E_20221201_NO30_400nL_HLAc1_4e7_PDAC_Slot2-6_1_3524.15163.503.1 1 3 1 15.48 8.14 6.91 7.34 90.5 7 0 0 31000.0 M40m Hydrogen 32 (V) 9 TPVAVITDm (K) 9 GPITVTInm T P/V\A|V|I|T\D\m 77.8 7 6 4 3 0 dual b/y bias m:Oxidized methionine 52.385532 1.424257 962.4839 946.491 15.9926 -2.4 0.0 3.8 21191.7 nuORFdb_human ENST00000354911.8_2_10:28822518-28879725:+ ENST00000354911.8_2_10:28822518-28879725:+ WAC Out-of-Frame Out-of-Frame GN=WAC Out-of-Frame E_20221201_NO30_400nL_HLAc1_4e7_PDAC_Slot2-6_1_3524 Exploris_FAIMS VH20221207_MKP_FAIMS2022E_HLAc1_4e7_PDAC.37551.37667.2 2 2 1 14.69 6.07 7.35 8.62 90.2 7 0 0 160000000.0 M40m Hydrogen 32 (V) 9 TPVAVITDm (K) 9 TVIGLPTnm T P\V\A\V\I|T/D|m 77.8 7 6 3 2 0 b-ion bias m:Oxidized methionine 1.0 100.0 70.13 11.56 -70.0 481.7456 946.491 15.9926 -2.4 0.20000000000000018 3.8 21191.7 nuORFdb_human ENST00000354911.8_2_10:28822518-28879725:+ ENST00000354911.8_2_10:28822518-28879725:+ WAC Out-of-Frame Out-of-Frame GN=WAC Out-of-Frame VH20221207_MKP_FAIMS2022E_HLAc1_4e7_PDAC Eva-50mio-3fr AL20210827_EV_HLA_PDAC_PANFRO413_2D_50e6_W632_classI_fxn2.25437.25437.2 2 1 1 15.33 5.24 11.66 10.09 66.1 9 0 0 16600000.0 Hydrogen 1528 (M) 11 TPWNQGATPAY (G) 11 GPLSPAWmPAY T P\W-N\Q\G\A\T|P/A/Y 81.8 9 6 3 1 1 b-ion bias 0.998 100.0 47.47 79.42 0.0 603.2833 1205.559 0.0007 0.6 0.3 5.19 213870.4 nuORFdb_human ENST00000617998.4_2_17:7388053-7417318:+ ENST00000617998.4_2_17:7388053-7417318:+ POLR2A 5' Overlap uORF 5' Overlap uORF GN=POLR2A 5' Overlap uORF AL20210827_EV_HLA_PDAC_PANFRO413_2D_50e6_W632_classI_fxn2 Exploris AL20221201_MKP_PDAC_01.34270.34406.2 2 1 1 9.13 1.2 8.77 7.93 60.9 7 0 0 136000000.0 Hydrogen 1528 (M) 11 TPWNQGATPAY (G) 11 GPLSPAWmPAY T P W N\Q\G\A\T|P/A/Y 63.6 7 5 3 1 0 unbiased 1.0 100.0 54.18 74.21 0.0 603.2827 1205.559 -0.0004 -0.3 -1.1 5.19 213870.4 nuORFdb_human ENST00000617998.4_2_17:7388053-7417318:+ ENST00000617998.4_2_17:7388053-7417318:+ POLR2A 5' Overlap uORF 5' Overlap uORF GN=POLR2A 5' Overlap uORF AL20221201_MKP_PDAC_01 SCP E_20221201_NO30_400nL_HLAc1_4e7_PDAC_Slot2-6_1_3524.16308.236.1 1 3 1 17.69 6.44 10.88 11.25 96.9 8 0 0 15800.0 Hydrogen 1528 (M) 11 TPWNQGATPAY (G) 11 PWAEnRTAmL T P/W/N|Q\G\A|T|P-A\Y 81.8 9 6 5 3 1 unbiased 56.339882 1.581785 1205.5545 1205.559 -0.0086 -7.1 -4.699999999999999 5.19 213870.4 nuORFdb_human ENST00000617998.4_2_17:7388053-7417318:+ ENST00000617998.4_2_17:7388053-7417318:+ POLR2A 5' Overlap uORF 5' Overlap uORF GN=POLR2A 5' Overlap uORF E_20221201_NO30_400nL_HLAc1_4e7_PDAC_Slot2-6_1_3524 Exploris_FAIMS VH20221207_MKP_FAIMS2022E_HLAc1_4e7_PDAC.41154.41293.2 2 1 1 15.56 3.18 10.14 12.38 80.0 9 0 0 59300000.0 Hydrogen 1528 (M) 11 TPWNQGATPAY (G) 11 DTGPELWGAPY T P\W-N\Q\G\A\T|P/A/Y 81.8 9 6 3 1 1 b-ion bias 0.998 95.3 75.57 8.44 -50.0 603.2807 1205.559 -0.0045 -3.7 -1.1 5.19 213870.4 nuORFdb_human ENST00000617998.4_2_17:7388053-7417318:+ ENST00000617998.4_2_17:7388053-7417318:+ POLR2A 5' Overlap uORF 5' Overlap uORF GN=POLR2A 5' Overlap uORF VH20221207_MKP_FAIMS2022E_HLAc1_4e7_PDAC Eva-50mio-3fr AL20210827_EV_HLA_PDAC_PANFRO413_2D_50e6_W632_classI_fxn2.35394.35394.2 2 1 1 9.0 5.71 3.0 3.29 72.6 6 0 0 2860000.0 Hydrogen 2 (M) 10 TPYTGPSIAL (-) 10 ALQEFDLAL T P Y\T\G\P S\I|A/L 50.0 5 5 2 1 0 b-ion bias 0.906 92.6 64.87 100.0 0.0 510.2746 1019.541 0.0011 1.0 0.7 5.19 1150.3 nuORFdb_human ENST00000334370.8_1_19:42830159-42830195:+ ENST00000334370.8_1_19:42830159-42830195:+ MEGF8 5' uORF 5' uORF GN=MEGF8 5' uORF AL20210827_EV_HLA_PDAC_PANFRO413_2D_50e6_W632_classI_fxn2 SCP E_20221201_NO30_400nL_HLAc1_4e7_PDAC_Slot2-6_1_3524.21839.390.1 1 1 1 8.05 4.18 6.64 3.87 62.3 7 0 0 14400.0 Hydrogen 2 (M) 10 TPYTGPSIAL (-) 10 VLSSLGEMAI T P/Y/T|G|P S\I\A\L 60.0 6 5 4 2 0 unbiased 75.447182 1.491071 1019.5414 1019.541 -0.0008 -0.8 1.5999999999999999 5.19 1150.3 nuORFdb_human ENST00000334370.8_1_19:42830159-42830195:+ ENST00000334370.8_1_19:42830159-42830195:+ MEGF8 5' uORF 5' uORF GN=MEGF8 5' uORF E_20221201_NO30_400nL_HLAc1_4e7_PDAC_Slot2-6_1_3524 40mio-rep1 E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep1_Slot1-04_1_3496.14984.1347.2 2 1 1 11.47 3.0 8.43 8.47 73.1 7 1 2 20700.0 Hydrogen 2 (M) 9 TSAKKMLLW (N) 9 FGIARVLNST T/S/A/K/K M\L|L\W 77.8 7 3 5 1 0 unbiased 51.759174 0.90966 539.3083 1077.612 -0.0038 -3.5 -0.2999999999999998 10.0 2739.3 nuORFdb_human ENST00000429544.6_1_3:129156740-129158582:- ENST00000429544.6_1_3:129156740-129158582:- MBD4 Out-of-Frame Out-of-Frame GN=MBD4 Out-of-Frame E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep1_Slot1-04_1_3496 20mio-rep1 E_20221201_NO30_400nL_HLAc1_2e7_directIP_titration_rep1_Slot2-4_1_3515.10291.1378.2 2 1 1 12.49 4.05 7.59 8.45 73.6 7 1 2 6860.0 M7m Hydrogen 2 (M) 9 TSAKKmLLW (N) 9 GmVLFVLLW T/S/A/K/K m\L|L\W 77.8 7 3 5 1 0 unbiased m:Oxidized methionine 35.546249 0.889879 547.3038 1077.612 15.9882 -6.1 0.10000000000000053 10.0 2739.3 nuORFdb_human ENST00000429544.6_1_3:129156740-129158582:- ENST00000429544.6_1_3:129156740-129158582:- MBD4 Out-of-Frame Out-of-Frame GN=MBD4 Out-of-Frame E_20221201_NO30_400nL_HLAc1_2e7_directIP_titration_rep1_Slot2-4_1_3515 40mio-rep1 E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep1_Slot1-04_1_3496.10578.1349.2 2 1 1 14.21 4.39 7.61 9.82 78.3 6 1 2 7830.0 M7m Hydrogen 2 (M) 9 TSAKKmLLW (N) 9 GmVLFVLLW T/S/A K/K m|L\L\W 55.6 5 3 4 1 0 unbiased m:Oxidized methionine 36.541813 0.90842 547.3072 1077.612 15.9942 -0.6 2.6 10.0 2739.3 nuORFdb_human ENST00000429544.6_1_3:129156740-129158582:- ENST00000429544.6_1_3:129156740-129158582:- MBD4 Out-of-Frame Out-of-Frame GN=MBD4 Out-of-Frame E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep1_Slot1-04_1_3496 40mio-rep2 E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep2_Slot2-5_1_3520.10268.1364.2 2 2 1 12.57 5.51 6.66 7.06 75.6 6 1 2 17400.0 M7m Hydrogen 2 (M) 9 TSAKKmLLW (N) 9 GmVLFVLLW T/S/A K/K m\L|L\W 55.6 5 3 4 1 0 unbiased m:Oxidized methionine 35.473038 0.898637 547.3046 1077.612 15.9902 -4.3 0.6000000000000005 10.0 2739.3 nuORFdb_human ENST00000429544.6_1_3:129156740-129158582:- ENST00000429544.6_1_3:129156740-129158582:- MBD4 Out-of-Frame Out-of-Frame GN=MBD4 Out-of-Frame E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep2_Slot2-5_1_3520 40mio-rep1 E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep1_Slot1-04_1_3496.11413.484.1 1 3 1 8.57 4.85 1.92 3.72 69.0 6 0 0 8860.0 Hydrogen 21 (R) 9 TSEEDSLGY (K) 9 cPELSLGY T S E/E/D|S\L\G\Y 66.7 6 4 3 1 0 unbiased 39.425091 1.43553 1000.4099 1000.411 -0.0006 -0.6 2.6 3.57 5012.3 nuORFdb_human ENST00000274341.8_1_5:82969298-83016672:- ENST00000274341.8_1_5:82969298-83016672:- HAPLN1 5' Overlap uORF 5' Overlap uORF GN=HAPLN1 5' Overlap uORF E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep1_Slot1-04_1_3496 40mio-rep2 E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep2_Slot2-5_1_3520.11100.505.1 1 2 1 8.37 3.58 3.33 4.79 72.7 6 0 0 3560.0 Hydrogen 21 (R) 9 TSEEDSLGY (K) 9 cPELSLGY T S E/E\D\S\L\G\Y 66.7 6 5 1 0 0 b-ion bias 38.345505 1.42283 1000.4085 1000.411 -0.0006 -0.6 4.300000000000001 3.57 5012.3 nuORFdb_human ENST00000274341.8_1_5:82969298-83016672:- ENST00000274341.8_1_5:82969298-83016672:- HAPLN1 5' Overlap uORF 5' Overlap uORF GN=HAPLN1 5' Overlap uORF E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep2_Slot2-5_1_3520 40mio-rep3 E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep3_Slot2-5_1_3521.11075.501.1 1 1 1 10.75 4.68 4.87 6.07 80.9 6 0 0 1850.0 Hydrogen 21 (R) 9 TSEEDSLGY (K) 9 cPELSLGY T S/E E\D\S|L\G\Y 55.6 5 5 2 1 0 b-ion bias 38.261822 1.425282 1000.4079 1000.411 -0.0006 -0.6 3.9999999999999996 3.57 5012.3 nuORFdb_human ENST00000274341.8_1_5:82969298-83016672:- ENST00000274341.8_1_5:82969298-83016672:- HAPLN1 5' Overlap uORF 5' Overlap uORF GN=HAPLN1 5' Overlap uORF E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep3_Slot2-5_1_3521 40mio-rep4 E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep4_Slot2-5_1_3522.11089.500.1 1 1 1 8.94 4.15 4.79 4.79 66.4 7 0 0 2470.0 Hydrogen 21 (R) 9 TSEEDSLGY (K) 9 cPELSLGY T S/E/E\D|S\L\G\Y 77.8 7 5 3 1 0 unbiased 38.295555 1.425751 1000.4067 1000.411 -0.0006 -0.6 4.1000000000000005 3.57 5012.3 nuORFdb_human ENST00000274341.8_1_5:82969298-83016672:- ENST00000274341.8_1_5:82969298-83016672:- HAPLN1 5' Overlap uORF 5' Overlap uORF GN=HAPLN1 5' Overlap uORF E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep4_Slot2-5_1_3522 20mio-rep1 E_20221201_NO30_400nL_HLAc1_2e7_directIP_titration_rep1_Slot2-4_1_3515.19782.1420.2 2 1 1 10.87 6.91 3.95 3.95 77.8 5 1 1 10600.0 Hydrogen 69 (F) 10 TSFKDVAVNF (T) 10 TENYLKVNF T S/F K D V\A|V|N\F 40.0 4 4 3 2 0 unbiased 68.31604 0.86382 564.2873 1127.573 -0.0064 -5.7 0.5 5.5 11234.7 nuORFdb_human ENST00000581904.6_1_17:18602500-18608042:+ ENST00000581904.6_1_17:18602500-18608042:+|ENST00000571708.5_1_17:18602742-18608042:+ TRIM16L ncRNA Processed Transcript lncRNA GN=TRIM16L ncRNA Processed Transcript E_20221201_NO30_400nL_HLAc1_2e7_directIP_titration_rep1_Slot2-4_1_3515 20mio-rep2 E_20221201_NO30_400nL_HLAc1_2e7_directIP_titration_rep3_Slot2-4_1_3518.19834.1415.2 2 2 1 9.56 4.18 5.05 5.39 61.4 6 1 1 13500.0 Hydrogen 69 (F) 10 TSFKDVAVNF (T) 10 mANLRFVNF T S/F K D\V\A|V|N\F 50.0 5 5 3 2 0 unbiased 68.511665 0.866962 564.2878 1127.573 -0.0044 -3.9 2.4 5.5 11234.7 nuORFdb_human ENST00000581904.6_1_17:18602500-18608042:+ ENST00000581904.6_1_17:18602500-18608042:+|ENST00000571708.5_1_17:18602742-18608042:+ TRIM16L ncRNA Processed Transcript lncRNA GN=TRIM16L ncRNA Processed Transcript E_20221201_NO30_400nL_HLAc1_2e7_directIP_titration_rep3_Slot2-4_1_3518 20mio-rep3 E_20221201_NO30_400nL_HLAc1_2e7_directIP_titration_rep4_Slot2-4_1_3519.19840.1416.2 2 2 1 10.0 6.42 3.58 3.58 73.6 5 1 1 15000.0 Hydrogen 69 (F) 10 TSFKDVAVNF (T) 10 mANLRFVNF T S/F K D V\A|V|N\F 40.0 4 4 3 2 0 unbiased 68.52269 0.866366 564.2867 1127.573 -0.0064 -5.7 0.09999999999999964 5.5 11234.7 nuORFdb_human ENST00000581904.6_1_17:18602500-18608042:+ ENST00000581904.6_1_17:18602500-18608042:+|ENST00000571708.5_1_17:18602742-18608042:+ TRIM16L ncRNA Processed Transcript lncRNA GN=TRIM16L ncRNA Processed Transcript E_20221201_NO30_400nL_HLAc1_2e7_directIP_titration_rep4_Slot2-4_1_3519 40mio-rep2 E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep2_Slot2-5_1_3520.19778.1406.2 2 1 1 12.08 6.54 5.54 5.54 81.8 5 1 1 7920.0 Hydrogen 69 (F) 10 TSFKDVAVNF (T) 10 TENYLKVNF T S/F K D V|A|V|N\F 40.0 4 4 4 3 0 unbiased 68.323685 0.872688 564.2868 1127.573 -0.0064 -5.7 -0.7999999999999998 5.5 11234.7 nuORFdb_human ENST00000581904.6_1_17:18602500-18608042:+ ENST00000581904.6_1_17:18602500-18608042:+|ENST00000571708.5_1_17:18602742-18608042:+ TRIM16L ncRNA Processed Transcript lncRNA GN=TRIM16L ncRNA Processed Transcript E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep2_Slot2-5_1_3520 40mio-rep3 E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep3_Slot2-5_1_3521.19792.1412.2 2 1 1 7.26 4.49 3.38 2.77 66.3 5 1 1 8840.0 Hydrogen 69 (F) 10 TSFKDVAVNF (T) 10 TENYLKVNF T S/F K D V\A\V|N\F 40.0 4 4 2 1 0 b-ion bias 68.371193 0.868819 564.2884 1127.573 -0.0044 -3.9 0.6999999999999997 5.5 11234.7 nuORFdb_human ENST00000581904.6_1_17:18602500-18608042:+ ENST00000581904.6_1_17:18602500-18608042:+|ENST00000571708.5_1_17:18602742-18608042:+ TRIM16L ncRNA Processed Transcript lncRNA GN=TRIM16L ncRNA Processed Transcript E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep3_Slot2-5_1_3521 40mio-rep4 E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep4_Slot2-5_1_3522.19785.1409.2 2 2 1 7.83 3.4 4.48 4.43 57.4 5 1 1 14400.0 Hydrogen 69 (F) 10 TSFKDVAVNF (T) 10 TENYLKVNF T S/F K D V|A\V|N\F 40.0 4 4 3 2 0 unbiased 68.330772 0.870672 564.2867 1127.573 -0.0064 -5.7 -1.0 5.5 11234.7 nuORFdb_human ENST00000581904.6_1_17:18602500-18608042:+ ENST00000581904.6_1_17:18602500-18608042:+|ENST00000571708.5_1_17:18602742-18608042:+ TRIM16L ncRNA Processed Transcript lncRNA GN=TRIM16L ncRNA Processed Transcript E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep4_Slot2-5_1_3522 40mio-rep1 E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep1_Slot1-04_1_3496.25752.353.1 1 1 1 15.2 7.86 10.4 7.34 91.9 8 0 0 4500.0 Hydrogen 3 (Q) 10 TSSLPVNGLW (R) 10 KmVNVPLEQ T S/S/L|P\V|N\G\L\W 80.0 8 6 4 2 0 unbiased 88.962387 1.513264 1073.5553 1073.563 -0.0026 -2.4 0.8000000000000003 5.18 5278.0 nuORFdb_human ENST00000313608.12_1_17:55918309-55918571:- ENST00000313608.12_1_17:55918309-55918571:- MRPS23 Out-of-Frame Out-of-Frame GN=MRPS23 Out-of-Frame E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep1_Slot1-04_1_3496 40mio-rep4 E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep4_Slot2-5_1_3522.25553.1483.2 2 5 1 18.33 10.13 11.15 8.2 87.7 8 0 0 19200.0 Hydrogen 3 (Q) 10 TSSLPVNGLW (R) 10 VSGLEVNGIW T S/S/L|P|V|N|G|L\W 80.0 8 6 7 5 0 dual b/y bias 88.26062 0.823995 537.2813 1073.563 -0.0079 -7.3 -2.5999999999999996 5.18 5278.0 nuORFdb_human ENST00000313608.12_1_17:55918309-55918571:- ENST00000313608.12_1_17:55918309-55918571:- MRPS23 Out-of-Frame Out-of-Frame GN=MRPS23 Out-of-Frame E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep4_Slot2-5_1_3522 40mio-rep1 E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep1_Slot1-04_1_3496.16559.1436.2 2 2 1 10.84 2.04 4.03 8.79 67.8 7 1 1 13300.0 Hydrogen 48 (K) 9 TSSTIKTLW (Q) 9 STKAVLVDFG T/S/S/T|I/K T|L\W 77.8 7 3 6 2 0 y-ion bias 57.19978 0.85361 518.7859 1036.567 -0.0026 -2.5 0.7000000000000002 8.41 7820.8 smORF_v4|nuORFdb_human LOC645166_1:148932840-148951298:+ LOC645166_1:148932840-148951298:+|ENST00000539543.1_1:148932830-148951295:+|ENST00000636560.1_1_2:91825005-91843396:- LOC645166 lncRNA 1:148932840-148951298:+ 1 RP11-14N7.2 lincRNA lncRNA GN=LOC645166 E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep1_Slot1-04_1_3496 40mio-rep1 E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep1_Slot1-04_1_3496.18749.305.1 1 2 1 7.27 -1.3 7.14 8.57 59.1 7 0 0 7590.0 Hydrogen 43 (A) 11 TSWASSSAAGW (-) 11 ASRQcLAGW T S W/A|S/S S\A\A\G\W 54.5 6 5 3 1 0 unbiased 64.768707 1.541257 1110.4806 1110.485 -0.0051 -4.6 -1.3999999999999995 5.18 5652.4 nuORFdb_human ENST00000524230.6_3_3:126373628-126373787:- ENST00000524230.6_3_3:126373628-126373787:- TXNRD3 Out-of-Frame Out-of-Frame GN=TXNRD3 Out-of-Frame E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep1_Slot1-04_1_3496 40mio-rep2 E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep2_Slot2-5_1_3520.18448.319.1 1 2 1 8.41 2.5 7.77 5.91 66.2 7 0 0 4710.0 Hydrogen 43 (A) 11 TSWASSSAAGW (-) 11 GFQAECWRG T S W/A/S/S S\A\A\G\W 54.5 6 4 3 0 0 unbiased 63.729397 1.533374 1110.4765 1110.485 -0.0051 -4.6 0.3000000000000007 5.18 5652.4 nuORFdb_human ENST00000524230.6_3_3:126373628-126373787:- ENST00000524230.6_3_3:126373628-126373787:- TXNRD3 Out-of-Frame Out-of-Frame GN=TXNRD3 Out-of-Frame E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep2_Slot2-5_1_3520 40mio-rep3 E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep3_Slot2-5_1_3521.18475.1449.2 2 1 1 7.79 -1.83 9.67 9.62 66.5 7 0 0 3810.0 Hydrogen 43 (A) 11 TSWASSSAAGW (-) 11 FNTTWQEAN T S W\A|S/S/S A/A|G\W 54.5 6 4 5 2 0 unbiased 63.820789 0.845799 555.7456 1110.485 -0.0003 -0.3 4.3 5.18 5652.4 nuORFdb_human ENST00000524230.6_3_3:126373628-126373787:- ENST00000524230.6_3_3:126373628-126373787:- TXNRD3 Out-of-Frame Out-of-Frame GN=TXNRD3 Out-of-Frame E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep3_Slot2-5_1_3521 40mio-rep4 E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep4_Slot2-5_1_3522.18481.1447.2 2 2 1 13.99 0.43 12.15 13.56 80.0 8 0 0 5270.0 Hydrogen 43 (A) 11 TSWASSSAAGW (-) 11 ASRQcLAGW T S W/A|S/S|S/A|A|G\W 72.7 8 5 7 4 0 dual b/y bias 63.824871 0.847134 555.7433 1110.485 -0.0063 -5.7 -1.0 5.18 5652.4 nuORFdb_human ENST00000524230.6_3_3:126373628-126373787:- ENST00000524230.6_3_3:126373628-126373787:- TXNRD3 Out-of-Frame Out-of-Frame GN=TXNRD3 Out-of-Frame E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep4_Slot2-5_1_3522 40mio-rep1 E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep1_Slot1-04_1_3496.25525.1371.2 2 2 1 16.35 3.45 13.61 12.91 95.1 7 0 1 13000.0 Hydrogen 36 (I) 9 TSWFVPRPW (-) 9 AVMPRLGIGmD T/S/W|F|V|P/R|P W 77.8 7 4 7 4 0 dual b/y bias 88.178596 0.894547 588.3012 1175.6 -0.0049 -4.2 -1.0 9.41 4837.6 nuORFdb_human ENST00000372209.3_1_1:45241798-45243366:+ ENST00000372209.3_1_1:45241798-45243366:+ RPS8 Out-of-Frame Out-of-Frame GN=RPS8 Out-of-Frame E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep1_Slot1-04_1_3496 10mio-rep1 E_20221201_NO30_400nL_HLAc1_1e7_directIP_titration_rep1_Slot2-3_1_3512.17917.1484.2 2 1 1 8.07 3.94 1.06 4.14 73.0 5 1 1 5070.0 Hydrogen 125 (M) 8 TSYLPTRW (-) 8 TSLYRTPW T/S/Y|L|P/T R W 62.5 5 2 5 2 0 y-ion bias 61.89365 0.823626 512.2649 1023.526 -0.0031 -3.0 4.7 8.41 14708.2 nuORFdb_human ENST00000456708.5_1_2:220090211-220094280:- ENST00000456708.5_1_2:220090211-220094280:- ATG9A Nonsense Mediated Decay Other GN=ATG9A Nonsense Mediated Decay E_20221201_NO30_400nL_HLAc1_1e7_directIP_titration_rep1_Slot2-3_1_3512 10mio-rep2 E_20221201_NO30_400nL_HLAc1_1e7_directIP_titration_rep2_Slot2-3_1_3513.17848.1481.2 2 1 1 8.65 5.6 2.11 3.05 73.5 5 1 1 4120.0 Hydrogen 125 (M) 8 TSYLPTRW (-) 8 TSLYRTPW T/S/Y|L|P T R\W 62.5 5 3 4 2 0 unbiased 61.652107 0.825557 512.2647 1023.526 -0.0031 -3.0 3.7 8.41 14708.2 nuORFdb_human ENST00000456708.5_1_2:220090211-220094280:- ENST00000456708.5_1_2:220090211-220094280:- ATG9A Nonsense Mediated Decay Other GN=ATG9A Nonsense Mediated Decay E_20221201_NO30_400nL_HLAc1_1e7_directIP_titration_rep2_Slot2-3_1_3513 40mio-rep1 E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep1_Slot1-04_1_3496.18053.1452.2 2 1 1 11.89 7.74 2.86 4.15 87.0 5 1 1 20100.0 Hydrogen 125 (M) 8 TSYLPTRW (-) 8 TSLYRTPW T/S/Y|L|P T R\W 62.5 5 3 4 2 0 unbiased 62.361534 0.843715 512.2646 1023.526 -0.0031 -3.0 0.20000000000000018 8.41 14708.2 nuORFdb_human ENST00000456708.5_1_2:220090211-220094280:- ENST00000456708.5_1_2:220090211-220094280:- ATG9A Nonsense Mediated Decay Other GN=ATG9A Nonsense Mediated Decay E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep1_Slot1-04_1_3496 10mio-rep1 E_20221201_NO30_400nL_HLAc1_1e7_directIP_titration_rep1_Slot2-3_1_3512.7074.1485.2 2 2 1 10.89 7.58 5.05 3.31 80.0 6 1 1 6400.0 Hydrogen 33 (P) 10 TTAAGSPRTW (L) 10 PSRAFSQPGT T/T/A/A/G|S|P-R T W 70.0 7 2 6 2 1 y-ion bias 24.446484 0.822666 524.2591 1047.522 -0.0111 -10.6 -2.8999999999999995 9.41 5957.7 nuORFdb_human ENST00000484515.1_1_20:4158069-4162462:+ ENST00000484515.1_1_20:4158069-4162462:+ SMOX ncRNA Processed Transcript lncRNA GN=SMOX ncRNA Processed Transcript E_20221201_NO30_400nL_HLAc1_1e7_directIP_titration_rep1_Slot2-3_1_3512 10mio-rep2 E_20221201_NO30_400nL_HLAc1_1e7_directIP_titration_rep2_Slot2-3_1_3513.7008.1484.2 2 1 1 13.43 8.48 5.68 4.96 87.5 7 1 1 2280.0 Hydrogen 33 (P) 10 TTAAGSPRTW (L) 10 GMLVSASAGAPS T/T/A|A|G|S|P R T\W 70.0 7 5 6 4 0 dual b/y bias 24.216776 0.823476 524.2625 1047.522 -0.0051 -4.8 1.9000000000000004 9.41 5957.7 nuORFdb_human ENST00000484515.1_1_20:4158069-4162462:+ ENST00000484515.1_1_20:4158069-4162462:+ SMOX ncRNA Processed Transcript lncRNA GN=SMOX ncRNA Processed Transcript E_20221201_NO30_400nL_HLAc1_1e7_directIP_titration_rep2_Slot2-3_1_3513 10mio-rep3 E_20221201_NO30_400nL_HLAc1_1e7_directIP_titration_rep3_Slot2-3_1_3514.7019.1493.2 2 1 1 8.95 6.14 2.54 2.81 78.3 6 1 1 2880.0 Hydrogen 33 (P) 10 TTAAGSPRTW (L) 10 TTAASWRPTG T/T/A|A|G/S/P R T W 60.0 6 2 6 2 0 y-ion bias 24.255367 0.818145 524.2609 1047.522 -0.0071 -6.7 -0.40000000000000036 9.41 5957.7 nuORFdb_human ENST00000484515.1_1_20:4158069-4162462:+ ENST00000484515.1_1_20:4158069-4162462:+ SMOX ncRNA Processed Transcript lncRNA GN=SMOX ncRNA Processed Transcript E_20221201_NO30_400nL_HLAc1_1e7_directIP_titration_rep3_Slot2-3_1_3514 20mio-rep1 E_20221201_NO30_400nL_HLAc1_2e7_directIP_titration_rep1_Slot2-4_1_3515.6940.1484.2 2 1 1 10.82 6.3 4.4 4.52 80.0 6 1 1 5300.0 Hydrogen 33 (P) 10 TTAAGSPRTW (L) 10 GMLVSASAGAPS T/T/A/A|G|S/P-R T W 70.0 7 2 6 2 1 y-ion bias 23.976549 0.823853 524.2607 1047.522 -0.0071 -6.7 -0.5 9.41 5957.7 nuORFdb_human ENST00000484515.1_1_20:4158069-4162462:+ ENST00000484515.1_1_20:4158069-4162462:+ SMOX ncRNA Processed Transcript lncRNA GN=SMOX ncRNA Processed Transcript E_20221201_NO30_400nL_HLAc1_2e7_directIP_titration_rep1_Slot2-4_1_3515 20mio-rep2 E_20221201_NO30_400nL_HLAc1_2e7_directIP_titration_rep3_Slot2-4_1_3518.7102.1477.2 2 3 1 12.09 7.72 3.58 4.37 82.1 6 1 1 16700.0 Hydrogen 33 (P) 10 TTAAGSPRTW (L) 10 GMLVSASAGAPS T/T/A|A|G|S/P-R T W 70.0 7 3 6 3 1 y-ion bias 24.537779 0.82779 524.2597 1047.522 -0.0091 -8.6 -2.3 9.41 5957.7 nuORFdb_human ENST00000484515.1_1_20:4158069-4162462:+ ENST00000484515.1_1_20:4158069-4162462:+ SMOX ncRNA Processed Transcript lncRNA GN=SMOX ncRNA Processed Transcript E_20221201_NO30_400nL_HLAc1_2e7_directIP_titration_rep3_Slot2-4_1_3518 20mio-rep3 E_20221201_NO30_400nL_HLAc1_2e7_directIP_titration_rep4_Slot2-4_1_3519.6979.1482.2 2 1 1 12.2 6.84 5.19 5.36 84.3 7 1 1 4900.0 Hydrogen 33 (P) 10 TTAAGSPRTW (L) 10 TTAASWRPTG T/T/A/A|G|S/P R T\W 70.0 7 3 6 2 0 y-ion bias 24.110481 0.824855 524.2616 1047.522 -0.0051 -4.8 1.0 9.41 5957.7 nuORFdb_human ENST00000484515.1_1_20:4158069-4162462:+ ENST00000484515.1_1_20:4158069-4162462:+ SMOX ncRNA Processed Transcript lncRNA GN=SMOX ncRNA Processed Transcript E_20221201_NO30_400nL_HLAc1_2e7_directIP_titration_rep4_Slot2-4_1_3519 40mio-rep1 E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep1_Slot1-04_1_3496.7208.1465.2 2 1 1 14.35 8.81 5.16 5.54 88.1 8 1 1 15200.0 Hydrogen 33 (P) 10 TTAAGSPRTW (L) 10 GMLVSASAGAPS T/T/A|A|G|S/P/R T\W 80.0 8 4 7 3 0 unbiased 24.906916 0.835371 524.2621 1047.522 -0.0051 -4.8 -1.5999999999999996 9.41 5957.7 nuORFdb_human ENST00000484515.1_1_20:4158069-4162462:+ ENST00000484515.1_1_20:4158069-4162462:+ SMOX ncRNA Processed Transcript lncRNA GN=SMOX ncRNA Processed Transcript E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep1_Slot1-04_1_3496 40mio-rep2 E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep2_Slot2-5_1_3520.6952.1474.2 2 1 1 15.52 8.99 6.32 6.53 90.5 6 1 1 7510.0 Hydrogen 33 (P) 10 TTAAGSPRTW (L) 10 TTAASWRPTG T/T/A|A|G|S|P-R T W 70.0 7 4 6 4 1 dual b/y bias 24.021763 0.830102 524.262 1047.522 -0.0051 -4.8 0.10000000000000053 9.41 5957.7 nuORFdb_human ENST00000484515.1_1_20:4158069-4162462:+ ENST00000484515.1_1_20:4158069-4162462:+ SMOX ncRNA Processed Transcript lncRNA GN=SMOX ncRNA Processed Transcript E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep2_Slot2-5_1_3520 40mio-rep3 E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep3_Slot2-5_1_3521.6966.1473.2 2 1 1 15.44 9.22 6.77 6.23 92.0 7 1 1 10700.0 Hydrogen 33 (P) 10 TTAAGSPRTW (L) 10 PSRAFSQPGT T/T/A|A|G|S|P-R T\W 80.0 8 5 6 4 1 dual b/y bias 24.066402 0.830545 524.261 1047.522 -0.0071 -6.7 -2.1000000000000005 9.41 5957.7 nuORFdb_human ENST00000484515.1_1_20:4158069-4162462:+ ENST00000484515.1_1_20:4158069-4162462:+ SMOX ncRNA Processed Transcript lncRNA GN=SMOX ncRNA Processed Transcript E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep3_Slot2-5_1_3521 40mio-rep4 E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep4_Slot2-5_1_3522.6956.1477.2 2 1 1 12.74 7.77 4.44 4.97 85.7 7 1 1 8200.0 Hydrogen 33 (P) 10 TTAAGSPRTW (L) 10 TTAASWRPTG T/T/A|A|G/S/P-R T\W 80.0 8 3 6 2 1 y-ion bias 24.027632 0.827703 524.2604 1047.522 -0.0091 -8.6 -3.8999999999999995 9.41 5957.7 nuORFdb_human ENST00000484515.1_1_20:4158069-4162462:+ ENST00000484515.1_1_20:4158069-4162462:+ SMOX ncRNA Processed Transcript lncRNA GN=SMOX ncRNA Processed Transcript E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep4_Slot2-5_1_3522 10mio-rep1 E_20221201_NO30_400nL_HLAc1_1e7_directIP_titration_rep1_Slot2-3_1_3512.28117.293.1 1 1 1 11.17 2.87 6.54 8.3 73.9 6 0 0 1830.0 Hydrogen 12 (D) 9 TTDIVFFTY (R) 9 RMKNFFTY T T D\I\V|F\F|T\Y 66.7 6 6 2 2 0 b-ion bias 97.115425 1.548727 1106.5318 1106.541 -0.0105 -9.4 -1.7000000000000002 3.8 6284.4 nuORFdb_human ENST00000379774.4_1_17:10608340-10608511:+ ENST00000379774.4_1_17:10608340-10608511:+ ADPRM Out-of-Frame Out-of-Frame GN=ADPRM Out-of-Frame E_20221201_NO30_400nL_HLAc1_1e7_directIP_titration_rep1_Slot2-3_1_3512 20mio-rep1 E_20221201_NO30_400nL_HLAc1_2e7_directIP_titration_rep1_Slot2-4_1_3515.28076.289.1 1 1 1 10.24 -1.23 6.11 11.47 75.1 6 0 0 6260.0 Hydrogen 12 (D) 9 TTDIVFFTY (R) 9 FISnITQAnP T T D\I|V\F\F|T\Y 66.7 6 6 2 2 0 b-ion bias 96.949295 1.550887 1106.5331 1106.541 -0.0105 -9.4 -3.2 3.8 6284.4 nuORFdb_human ENST00000379774.4_1_17:10608340-10608511:+ ENST00000379774.4_1_17:10608340-10608511:+ ADPRM Out-of-Frame Out-of-Frame GN=ADPRM Out-of-Frame E_20221201_NO30_400nL_HLAc1_2e7_directIP_titration_rep1_Slot2-4_1_3515 40mio-rep2 E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep2_Slot2-5_1_3520.28073.274.1 1 2 1 12.3 2.41 7.93 9.89 80.2 6 0 0 17100.0 Hydrogen 12 (D) 9 TTDIVFFTY (R) 9 STEIVEGTRD T T D\I|V|F\F\T\Y 66.7 6 6 2 2 0 b-ion bias 96.980606 1.559672 1106.5353 1106.541 -0.0005 -0.4 4.5 3.8 6284.4 nuORFdb_human ENST00000379774.4_1_17:10608340-10608511:+ ENST00000379774.4_1_17:10608340-10608511:+ ADPRM Out-of-Frame Out-of-Frame GN=ADPRM Out-of-Frame E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep2_Slot2-5_1_3520 40mio-rep3 E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep3_Slot2-5_1_3521.28075.273.1 1 2 1 10.67 2.13 7.0 8.54 77.0 6 0 0 18700.0 Hydrogen 12 (D) 9 TTDIVFFTY (R) 9 RMKNFFTY T T D\I\V|F\F\T\Y 66.7 6 6 1 1 0 b-ion bias 96.980415 1.560285 1106.5356 1106.541 -0.0005 -0.4 4.199999999999999 3.8 6284.4 nuORFdb_human ENST00000379774.4_1_17:10608340-10608511:+ ENST00000379774.4_1_17:10608340-10608511:+ ADPRM Out-of-Frame Out-of-Frame GN=ADPRM Out-of-Frame E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep3_Slot2-5_1_3521 40mio-rep4 E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep4_Slot2-5_1_3522.28077.274.1 1 1 1 10.99 1.72 5.97 9.27 80.0 6 0 0 5480.0 Hydrogen 12 (D) 9 TTDIVFFTY (R) 9 TEAQFTLPEA T T D\I\V|F\F\T\Y 66.7 6 6 1 1 0 b-ion bias 96.977539 1.559751 1106.5374 1106.541 -0.0005 -0.4 4.3 3.8 6284.4 nuORFdb_human ENST00000379774.4_1_17:10608340-10608511:+ ENST00000379774.4_1_17:10608340-10608511:+ ADPRM Out-of-Frame Out-of-Frame GN=ADPRM Out-of-Frame E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep4_Slot2-5_1_3522 20mio-rep1 E_20221201_NO30_400nL_HLAc1_2e7_directIP_titration_rep1_Slot2-4_1_3515.17612.474.1 1 2 1 7.98 2.41 6.04 5.57 61.1 7 0 0 6030.0 Hydrogen 175 (A) 9 TTFTGTTSW (C) 9 STAFSGLDFG T T/F/T\G|T\T|S\W 77.8 7 5 4 2 0 unbiased 60.825188 1.441154 1001.4498 1001.457 -0.0075 -7.4 -1.2000000000000002 5.18 24569.4 nuORFdb_human ENST00000373045.10_1_1:38259693-38260377:+ ENST00000373045.10_1_1:38259693-38260377:+ MANEAL 5' Overlap uORF 5' Overlap uORF GN=MANEAL 5' Overlap uORF E_20221201_NO30_400nL_HLAc1_2e7_directIP_titration_rep1_Slot2-4_1_3515 40mio-rep1 E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep1_Slot1-04_1_3496.17889.447.1 1 1 1 7.44 3.78 3.96 3.66 62.2 6 0 0 9510.0 Hydrogen 175 (A) 9 TTFTGTTSW (C) 9 STAFSGLDFG T T F/T|G\T|T\S\W 66.7 6 5 3 2 0 unbiased 61.795269 1.457659 1001.4542 1001.457 -0.0075 -7.4 -4.2 5.18 24569.4 nuORFdb_human ENST00000373045.10_1_1:38259693-38260377:+ ENST00000373045.10_1_1:38259693-38260377:+ MANEAL 5' Overlap uORF 5' Overlap uORF GN=MANEAL 5' Overlap uORF E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep1_Slot1-04_1_3496 40mio-rep3 E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep3_Slot2-5_1_3521.17585.461.1 1 1 1 7.74 2.29 5.28 5.45 63.4 7 0 0 4950.0 Hydrogen 175 (A) 9 TTFTGTTSW (C) 9 STAFSGLDFG T T/F/T|G|T\T\S\W 77.8 7 5 4 2 0 unbiased 60.747707 1.449198 1001.4532 1001.457 -0.0075 -7.4 -2.8000000000000007 5.18 24569.4 nuORFdb_human ENST00000373045.10_1_1:38259693-38260377:+ ENST00000373045.10_1_1:38259693-38260377:+ MANEAL 5' Overlap uORF 5' Overlap uORF GN=MANEAL 5' Overlap uORF E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep3_Slot2-5_1_3521 40mio-rep4 E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep4_Slot2-5_1_3522.17561.459.1 1 1 1 7.79 3.22 6.92 4.57 60.3 7 0 0 5380.0 Hydrogen 175 (A) 9 TTFTGTTSW (C) 9 STAFSGLDFG T T/F|T/G\T|T\S\W 77.8 7 5 4 2 0 unbiased 60.64679 1.450121 1001.4505 1001.457 -0.0075 -7.4 -2.7 5.18 24569.4 nuORFdb_human ENST00000373045.10_1_1:38259693-38260377:+ ENST00000373045.10_1_1:38259693-38260377:+ MANEAL 5' Overlap uORF 5' Overlap uORF GN=MANEAL 5' Overlap uORF E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep4_Slot2-5_1_3522 10mio-rep1 E_20221201_NO30_400nL_HLAc1_1e7_directIP_titration_rep1_Slot2-3_1_3512.18107.1469.2 2 3 1 10.75 6.71 4.6 4.04 89.3 7 1 1 34700.0 Hydrogen 4 (S) 9 TTLPLGRSW (L) 9 LGGVSFGPLPS T T/L/P/L/G/R|S\W 77.8 7 2 6 1 0 y-ion bias 62.549435 0.833183 515.7829 1030.568 -0.0093 -9.0 -1.2999999999999998 9.41 1722.0 nuORFdb_human ENST00000252512.13_1_8:21827660-21827708:+ ENST00000252512.13_1_8:21827660-21827708:+ XPO7 Out-of-Frame Out-of-Frame GN=XPO7 Out-of-Frame E_20221201_NO30_400nL_HLAc1_1e7_directIP_titration_rep1_Slot2-3_1_3512 20mio-rep1 E_20221201_NO30_400nL_HLAc1_2e7_directIP_titration_rep1_Slot2-4_1_3515.18042.1457.2 2 2 1 12.37 8.32 4.61 4.06 94.2 8 1 1 43900.0 Hydrogen 4 (S) 9 TTLPLGRSW (L) 9 YPPFLPLPS T/T/L|P/L|G/R-S\W 100.0 9 3 6 2 1 y-ion bias 62.309818 0.840566 515.7841 1030.568 -0.0073 -7.1 -0.8999999999999995 9.41 1722.0 nuORFdb_human ENST00000252512.13_1_8:21827660-21827708:+ ENST00000252512.13_1_8:21827660-21827708:+ XPO7 Out-of-Frame Out-of-Frame GN=XPO7 Out-of-Frame E_20221201_NO30_400nL_HLAc1_2e7_directIP_titration_rep1_Slot2-4_1_3515 20mio-rep3 E_20221201_NO30_400nL_HLAc1_2e7_directIP_titration_rep4_Slot2-4_1_3519.18069.1448.2 2 3 1 12.85 9.31 5.64 3.54 93.2 6 1 1 48700.0 Hydrogen 4 (S) 9 TTLPLGRSW (L) 9 YPPFLPLPS T T/L|P|L|G R\S\W 55.6 5 5 4 3 0 dual b/y bias 62.407913 0.846219 515.7852 1030.568 -0.0053 -5.1 0.7000000000000002 9.41 1722.0 nuORFdb_human ENST00000252512.13_1_8:21827660-21827708:+ ENST00000252512.13_1_8:21827660-21827708:+ XPO7 Out-of-Frame Out-of-Frame GN=XPO7 Out-of-Frame E_20221201_NO30_400nL_HLAc1_2e7_directIP_titration_rep4_Slot2-4_1_3519 40mio-rep2 E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep2_Slot2-5_1_3520.17987.1440.2 2 3 1 13.92 10.3 5.75 3.62 92.1 7 1 1 83000.0 Hydrogen 4 (S) 9 TTLPLGRSW (L) 9 TTLPGRLCL T/T/L|P|L|G R|S\W 77.8 7 5 6 4 0 dual b/y bias 62.135078 0.851543 515.785 1030.568 -0.0053 -5.1 -0.1999999999999993 9.41 1722.0 nuORFdb_human ENST00000252512.13_1_8:21827660-21827708:+ ENST00000252512.13_1_8:21827660-21827708:+ XPO7 Out-of-Frame Out-of-Frame GN=XPO7 Out-of-Frame E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep2_Slot2-5_1_3520 40mio-rep3 E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep3_Slot2-5_1_3521.18064.1443.2 2 1 1 10.78 8.25 3.1 2.53 90.6 6 1 1 38700.0 Hydrogen 4 (S) 9 TTLPLGRSW (L) 9 YPPFLPLPS T/T/L|P/L|G R/S W 55.6 5 2 6 2 0 y-ion bias 62.401924 0.849557 515.7852 1030.568 -0.0053 -5.1 -0.5 9.41 1722.0 nuORFdb_human ENST00000252512.13_1_8:21827660-21827708:+ ENST00000252512.13_1_8:21827660-21827708:+ XPO7 Out-of-Frame Out-of-Frame GN=XPO7 Out-of-Frame E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep3_Slot2-5_1_3521 40mio-rep4 E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep4_Slot2-5_1_3522.18020.1440.2 2 2 1 14.63 10.01 5.63 4.62 95.1 7 1 1 57500.0 Hydrogen 4 (S) 9 TTLPLGRSW (L) 9 TTLPGRLCL T/T/L|P|L|G R|S\W 77.8 7 5 6 4 0 dual b/y bias 62.232368 0.85127 515.7849 1030.568 -0.0053 -5.1 -0.39999999999999947 9.41 1722.0 nuORFdb_human ENST00000252512.13_1_8:21827660-21827708:+ ENST00000252512.13_1_8:21827660-21827708:+ XPO7 Out-of-Frame Out-of-Frame GN=XPO7 Out-of-Frame E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep4_Slot2-5_1_3522 5mio-rep1 E_20221201_NO30_400nL_HLAc1_5e6_directIP_titration_rep1_Slot1-37_1_3502.18360.1489.2 2 3 1 10.72 8.24 3.57 2.49 86.9 8 1 1 8530.0 Hydrogen 4 (S) 9 TTLPLGRSW (L) 9 YPPFLPLPS T/T/L/P/L|G/R-S\W 100.0 9 2 6 1 1 y-ion bias 63.416332 0.820278 515.7821 1030.568 -0.0113 -10.9 -3.9000000000000004 9.41 1722.0 nuORFdb_human ENST00000252512.13_1_8:21827660-21827708:+ ENST00000252512.13_1_8:21827660-21827708:+ XPO7 Out-of-Frame Out-of-Frame GN=XPO7 Out-of-Frame E_20221201_NO30_400nL_HLAc1_5e6_directIP_titration_rep1_Slot1-37_1_3502 40mio-rep1 E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep1_Slot1-04_1_3496.28299.1318.2 2 2 1 11.91 8.96 5.62 2.95 81.8 6 1 1 13000.0 Hydrogen 95 (T) 10 TTMKELLSFW (M) 10 TImLAASISFW T T M/K E\L|L|S|F\W 50.0 5 5 4 3 0 unbiased 97.764793 0.927768 628.3212 1255.639 -0.0044 -3.5 -0.2999999999999998 5.66 12652.7 nuORFdb_human ENST00000496455.6_1_3:155588441-155611400:+ ENST00000496455.6_1_3:155588441-155611400:+ GMPS 5' Overlap uORF 5' Overlap uORF GN=GMPS 5' Overlap uORF E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep1_Slot1-04_1_3496 20mio-rep1 E_20221201_NO30_400nL_HLAc1_2e7_directIP_titration_rep1_Slot2-4_1_3515.24718.1319.2 2 1 1 8.19 6.54 1.84 1.64 60.5 6 1 1 1960.0 M97m Hydrogen 95 (T) 10 TTmKELLSFW (M) 10 LFSITSPSSFW T T/m K/E L\L|S|F\W 40.0 4 4 4 2 0 unbiased m:Oxidized methionine 85.356674 0.926767 636.3166 1255.639 15.9876 -5.8 0.40000000000000036 5.66 12652.7 nuORFdb_human ENST00000496455.6_1_3:155588441-155611400:+ ENST00000496455.6_1_3:155588441-155611400:+ GMPS 5' Overlap uORF 5' Overlap uORF GN=GMPS 5' Overlap uORF E_20221201_NO30_400nL_HLAc1_2e7_directIP_titration_rep1_Slot2-4_1_3515 40mio-rep2 E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep2_Slot2-5_1_3520.24838.1305.2 2 2 1 9.08 4.91 2.32 4.17 69.9 6 1 1 7950.0 M97m Hydrogen 95 (T) 10 TTmKELLSFW (M) 10 LFSITSPSSFW T T m\K\E L|L|S|F\W 50.0 5 6 3 3 0 b-ion bias m:Oxidized methionine 85.80172 0.935969 636.3166 1255.639 15.9876 -5.8 -0.8999999999999995 5.66 12652.7 nuORFdb_human ENST00000496455.6_1_3:155588441-155611400:+ ENST00000496455.6_1_3:155588441-155611400:+ GMPS 5' Overlap uORF 5' Overlap uORF GN=GMPS 5' Overlap uORF E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep2_Slot2-5_1_3520 40mio-rep1 E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep1_Slot1-04_1_3496.21220.496.1 1 1 1 11.35 6.92 4.55 4.43 84.3 7 0 0 3620.0 Hydrogen 37 (G) 9 TTVPSSISW (K) 9 TSLSPSLLC T T/V/P\S\S\I\S\W 77.8 7 5 2 0 0 b-ion bias 73.304474 1.428528 977.4881 977.494 -0.0058 -6.0 -2.8 5.18 5454.5 nuORFdb_human ENST00000380492.7_1_X:14937843-14937984:+ ENST00000380492.7_1_X:14937843-14937984:+ MOSPD2 3' Overlap dORF 3' Overlap dORF GN=MOSPD2 3' Overlap dORF E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep1_Slot1-04_1_3496 10mio-rep1 E_20221201_NO30_400nL_HLAc1_1e7_directIP_titration_rep1_Slot2-3_1_3512.16896.1423.2 2 1 1 9.95 7.09 4.62 2.86 73.1 7 1 1 5290.0 Hydrogen 36 (A) 9 TTVSWDRSW (-) 9 TLSSWnRSW T/T/V|S/W D|R/S\W 77.8 7 3 6 2 0 y-ion bias 58.366127 0.862142 569.2666 1137.532 -0.0056 -4.9 2.8 5.5 4913.6 nuORFdb_human ENST00000483205.1_1_3:47551669-47552697:- ENST00000483205.1_1_3:47551669-47552697:-|ENST00000461208.5_1_3:47545925-47552697:- ELP6 ncRNA Retained Intron lncRNA GN=ELP6 ncRNA Retained Intron E_20221201_NO30_400nL_HLAc1_1e7_directIP_titration_rep1_Slot2-3_1_3512 10mio-rep3 E_20221201_NO30_400nL_HLAc1_1e7_directIP_titration_rep3_Slot2-3_1_3514.16818.1421.2 2 1 1 9.05 6.3 2.23 2.75 60.5 5 1 1 5410.0 Hydrogen 36 (A) 9 TTVSWDRSW (-) 9 TLSSWnRSW T T/V|S|W D\R\S W 33.3 3 4 3 2 0 unbiased 58.095886 0.862922 569.2659 1137.532 -0.0076 -6.7 -0.40000000000000036 5.5 4913.6 nuORFdb_human ENST00000483205.1_1_3:47551669-47552697:- ENST00000483205.1_1_3:47551669-47552697:-|ENST00000461208.5_1_3:47545925-47552697:- ELP6 ncRNA Retained Intron lncRNA GN=ELP6 ncRNA Retained Intron E_20221201_NO30_400nL_HLAc1_1e7_directIP_titration_rep3_Slot2-3_1_3514 20mio-rep1 E_20221201_NO30_400nL_HLAc1_2e7_directIP_titration_rep1_Slot2-4_1_3515.16808.1416.2 2 1 1 9.45 5.66 4.25 3.79 69.6 6 1 1 5900.0 Hydrogen 36 (A) 9 TTVSWDRSW (-) 9 TLSSWnRSW T/T/V/S|W\D/R S W 66.7 6 2 5 1 0 y-ion bias 58.047489 0.866104 569.2667 1137.532 -0.0056 -4.9 1.2999999999999998 5.5 4913.6 nuORFdb_human ENST00000483205.1_1_3:47551669-47552697:- ENST00000483205.1_1_3:47551669-47552697:-|ENST00000461208.5_1_3:47545925-47552697:- ELP6 ncRNA Retained Intron lncRNA GN=ELP6 ncRNA Retained Intron E_20221201_NO30_400nL_HLAc1_2e7_directIP_titration_rep1_Slot2-4_1_3515 20mio-rep2 E_20221201_NO30_400nL_HLAc1_2e7_directIP_titration_rep3_Slot2-4_1_3518.16888.1415.2 2 1 1 8.99 5.66 4.55 3.33 62.0 8 1 1 9110.0 Hydrogen 36 (A) 9 TTVSWDRSW (-) 9 TLSSWnRSW T/T/V/S|W-D/R\S\W 100.0 9 3 5 1 1 unbiased 58.332088 0.866835 569.267 1137.532 -0.0056 -4.9 1.3999999999999995 5.5 4913.6 nuORFdb_human ENST00000483205.1_1_3:47551669-47552697:- ENST00000483205.1_1_3:47551669-47552697:-|ENST00000461208.5_1_3:47545925-47552697:- ELP6 ncRNA Retained Intron lncRNA GN=ELP6 ncRNA Retained Intron E_20221201_NO30_400nL_HLAc1_2e7_directIP_titration_rep3_Slot2-4_1_3518 20mio-rep3 E_20221201_NO30_400nL_HLAc1_2e7_directIP_titration_rep4_Slot2-4_1_3519.16847.1410.2 2 1 1 10.16 7.48 5.74 2.67 62.4 7 1 1 7470.0 Hydrogen 36 (A) 9 TTVSWDRSW (-) 9 TLSSWnRSW T/T/V|S|W D/R\S\W 77.8 7 4 5 2 0 unbiased 58.189514 0.870366 569.266 1137.532 -0.0076 -6.7 -0.9000000000000004 5.5 4913.6 nuORFdb_human ENST00000483205.1_1_3:47551669-47552697:- ENST00000483205.1_1_3:47551669-47552697:-|ENST00000461208.5_1_3:47545925-47552697:- ELP6 ncRNA Retained Intron lncRNA GN=ELP6 ncRNA Retained Intron E_20221201_NO30_400nL_HLAc1_2e7_directIP_titration_rep4_Slot2-4_1_3519 40mio-rep1 E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep1_Slot1-04_1_3496.17063.1374.2 2 1 1 10.91 7.68 3.9 3.23 74.6 5 1 1 16800.0 Hydrogen 36 (A) 9 TTVSWDRSW (-) 9 HPACVPSPATT T/T/V/S|W D R\S W 44.4 4 2 4 1 0 y-ion bias 58.939949 0.89276 569.2691 1137.532 -0.0016 -1.4 1.8000000000000003 5.5 4913.6 nuORFdb_human ENST00000483205.1_1_3:47551669-47552697:- ENST00000483205.1_1_3:47551669-47552697:-|ENST00000461208.5_1_3:47545925-47552697:- ELP6 ncRNA Retained Intron lncRNA GN=ELP6 ncRNA Retained Intron E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep1_Slot1-04_1_3496 40mio-rep3 E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep3_Slot2-5_1_3521.16822.1393.2 2 2 1 8.14 5.17 3.06 2.98 62.7 6 1 1 16100.0 Hydrogen 36 (A) 9 TTVSWDRSW (-) 9 TLSSWnRSW T/T/V|S/W D R/S\W 66.7 6 2 5 1 0 y-ion bias 58.110157 0.880654 569.2661 1137.532 -0.0076 -6.7 -2.1000000000000005 5.5 4913.6 nuORFdb_human ENST00000483205.1_1_3:47551669-47552697:- ENST00000483205.1_1_3:47551669-47552697:-|ENST00000461208.5_1_3:47545925-47552697:- ELP6 ncRNA Retained Intron lncRNA GN=ELP6 ncRNA Retained Intron E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep3_Slot2-5_1_3521 SCP E_20221201_NO30_400nL_HLAc1_4e7_PDAC_Slot2-6_1_3524.10570.540.1 1 1 1 11.1 1.38 5.97 9.73 79.3 7 0 0 4280.0 Hydrogen 72 (E) 9 TVAGVTNQM (K) 9 LVTNQSQM T V/A/G\V|T\N|Q\M 77.8 7 5 4 2 0 unbiased 36.517551 1.401869 920.4485 920.451 -0.0026 -2.8 -0.3999999999999999 5.18 18464.6 nuORFdb_human ENST00000399959.6_1_X:41200742-41202586:+ ENST00000399959.6_1_X:41200742-41202586:+ DDX3X Out-of-Frame Out-of-Frame GN=DDX3X Out-of-Frame E_20221201_NO30_400nL_HLAc1_4e7_PDAC_Slot2-6_1_3524 10mio-rep1 E_20221201_NO30_400nL_HLAc1_1e7_directIP_titration_rep1_Slot2-3_1_3512.11064.600.1 1 1 1 10.03 4.74 4.14 5.29 73.8 6 0 0 2710.0 Hydrogen 20 (Q) 9 TVDDEAPAV (-) 9 CGTSPGAAPV T V/D D\E|A|P\A\V 55.6 5 5 3 2 0 unbiased 38.226994 1.36599 916.4148 916.426 -0.0108 -11.8 -4.1000000000000005 3.49 3064.4 nuORFdb_human ENST00000330736.9_1_16:89383456-89383540:- ENST00000330736.9_1_16:89383456-89383540:-|ENST00000378330.6_1_16:89383456-89484700:-|ENST00000562816.5_1_16:89383456-89484700:- ANKRD11 5' uORF 5' uORF GN=ANKRD11 5' uORF E_20221201_NO30_400nL_HLAc1_1e7_directIP_titration_rep1_Slot2-3_1_3512 10mio-rep2 E_20221201_NO30_400nL_HLAc1_1e7_directIP_titration_rep2_Slot2-3_1_3513.11034.596.1 1 2 1 10.23 6.39 3.38 3.83 73.4 6 0 0 7510.0 Hydrogen 20 (Q) 9 TVDDEAPAV (-) 9 AAEEETPAV T V/D D\E|A|P\A\V 55.6 5 5 3 2 0 unbiased 38.121212 1.368716 916.4206 916.426 -0.0048 -5.3 1.4000000000000004 3.49 3064.4 nuORFdb_human ENST00000330736.9_1_16:89383456-89383540:- ENST00000330736.9_1_16:89383456-89383540:-|ENST00000378330.6_1_16:89383456-89484700:-|ENST00000562816.5_1_16:89383456-89484700:- ANKRD11 5' uORF 5' uORF GN=ANKRD11 5' uORF E_20221201_NO30_400nL_HLAc1_1e7_directIP_titration_rep2_Slot2-3_1_3513 10mio-rep3 E_20221201_NO30_400nL_HLAc1_1e7_directIP_titration_rep3_Slot2-3_1_3514.11005.600.1 1 1 1 9.61 7.14 2.85 2.47 70.8 5 0 0 5390.0 Hydrogen 20 (Q) 9 TVDDEAPAV (-) 9 AAEEETPAV T V D D\E|A|P\A\V 55.6 5 5 2 2 0 b-ion bias 38.021603 1.366221 916.4216 916.426 -0.0038 -4.2 2.0999999999999996 3.49 3064.4 nuORFdb_human ENST00000330736.9_1_16:89383456-89383540:- ENST00000330736.9_1_16:89383456-89383540:-|ENST00000378330.6_1_16:89383456-89484700:-|ENST00000562816.5_1_16:89383456-89484700:- ANKRD11 5' uORF 5' uORF GN=ANKRD11 5' uORF E_20221201_NO30_400nL_HLAc1_1e7_directIP_titration_rep3_Slot2-3_1_3514 20mio-rep1 E_20221201_NO30_400nL_HLAc1_2e7_directIP_titration_rep1_Slot2-4_1_3515.10943.597.1 1 1 1 10.01 5.75 3.08 4.25 78.5 5 0 0 5470.0 Hydrogen 20 (Q) 9 TVDDEAPAV (-) 9 CGTSPGAAPV T V D D\E|A|P\A\V 55.6 5 5 2 2 0 b-ion bias 37.796986 1.367879 916.4216 916.426 -0.0038 -4.2 2.0 3.49 3064.4 nuORFdb_human ENST00000330736.9_1_16:89383456-89383540:- ENST00000330736.9_1_16:89383456-89383540:-|ENST00000378330.6_1_16:89383456-89484700:-|ENST00000562816.5_1_16:89383456-89484700:- ANKRD11 5' uORF 5' uORF GN=ANKRD11 5' uORF E_20221201_NO30_400nL_HLAc1_2e7_directIP_titration_rep1_Slot2-4_1_3515 20mio-rep2 E_20221201_NO30_400nL_HLAc1_2e7_directIP_titration_rep3_Slot2-4_1_3518.11071.594.1 1 1 1 8.2 6.09 1.53 2.11 64.3 5 0 0 4680.0 Hydrogen 20 (Q) 9 TVDDEAPAV (-) 9 CGTSPGAAPV T V D D\E|A|P\A\V 55.6 5 5 2 2 0 b-ion bias 38.240314 1.370072 916.4172 916.426 -0.0088 -9.6 -3.3 3.49 3064.4 nuORFdb_human ENST00000330736.9_1_16:89383456-89383540:- ENST00000330736.9_1_16:89383456-89383540:-|ENST00000378330.6_1_16:89383456-89484700:-|ENST00000562816.5_1_16:89383456-89484700:- ANKRD11 5' uORF 5' uORF GN=ANKRD11 5' uORF E_20221201_NO30_400nL_HLAc1_2e7_directIP_titration_rep3_Slot2-4_1_3518 20mio-rep3 E_20221201_NO30_400nL_HLAc1_2e7_directIP_titration_rep4_Slot2-4_1_3519.10965.586.1 1 1 1 10.39 5.03 4.3 5.36 74.4 5 0 0 4680.0 Hydrogen 20 (Q) 9 TVDDEAPAV (-) 9 LSAEDnLGP T V/D D\E|A|P A\V 33.3 3 4 3 2 0 unbiased 37.880024 1.374446 916.4197 916.426 -0.0058 -6.3 -0.5 3.49 3064.4 nuORFdb_human ENST00000330736.9_1_16:89383456-89383540:- ENST00000330736.9_1_16:89383456-89383540:-|ENST00000378330.6_1_16:89383456-89484700:-|ENST00000562816.5_1_16:89383456-89484700:- ANKRD11 5' uORF 5' uORF GN=ANKRD11 5' uORF E_20221201_NO30_400nL_HLAc1_2e7_directIP_titration_rep4_Slot2-4_1_3519 40mio-rep1 E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep1_Slot1-04_1_3496.11257.573.1 1 1 1 12.52 6.38 4.11 6.14 78.1 7 0 0 7510.0 Hydrogen 20 (Q) 9 TVDDEAPAV (-) 9 AAEEETPAV T V/D\D\E|A|P\A\V 77.8 7 6 3 2 0 b-ion bias 38.88662 1.382526 916.4217 916.426 -0.0038 -4.2 -1.0 3.49 3064.4 nuORFdb_human ENST00000330736.9_1_16:89383456-89383540:- ENST00000330736.9_1_16:89383456-89383540:-|ENST00000378330.6_1_16:89383456-89484700:-|ENST00000562816.5_1_16:89383456-89484700:- ANKRD11 5' uORF 5' uORF GN=ANKRD11 5' uORF E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep1_Slot1-04_1_3496 40mio-rep2 E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep2_Slot2-5_1_3520.10940.587.1 1 1 1 10.44 5.83 3.48 4.6 72.4 5 0 0 6260.0 Hydrogen 20 (Q) 9 TVDDEAPAV (-) 9 ALDSEDAPV T V D D\E|A|P\A\V 55.6 5 5 2 2 0 b-ion bias 37.793228 1.374147 916.4211 916.426 -0.0048 -5.3 -0.39999999999999947 3.49 3064.4 nuORFdb_human ENST00000330736.9_1_16:89383456-89383540:- ENST00000330736.9_1_16:89383456-89383540:-|ENST00000378330.6_1_16:89383456-89484700:-|ENST00000562816.5_1_16:89383456-89484700:- ANKRD11 5' uORF 5' uORF GN=ANKRD11 5' uORF E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep2_Slot2-5_1_3520 40mio-rep3 E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep3_Slot2-5_1_3521.10937.588.1 1 1 1 9.18 5.3 2.77 3.89 72.8 6 0 0 9320.0 Hydrogen 20 (Q) 9 TVDDEAPAV (-) 9 AAEEETPAV T V/D D\E|A|P\A\V 55.6 5 5 3 2 0 unbiased 37.785526 1.373283 916.4208 916.426 -0.0048 -5.3 -0.7000000000000002 3.49 3064.4 nuORFdb_human ENST00000330736.9_1_16:89383456-89383540:- ENST00000330736.9_1_16:89383456-89383540:-|ENST00000378330.6_1_16:89383456-89484700:-|ENST00000562816.5_1_16:89383456-89484700:- ANKRD11 5' uORF 5' uORF GN=ANKRD11 5' uORF E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep3_Slot2-5_1_3521 40mio-rep4 E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep4_Slot2-5_1_3522.10932.585.1 1 1 1 7.51 2.93 3.4 4.58 55.2 6 0 0 1460.0 Hydrogen 20 (Q) 9 TVDDEAPAV (-) 9 APGSCSAVPA T V D\D\E|A|P\A\V 66.7 6 6 2 2 0 b-ion bias 37.7537 1.37517 916.4204 916.426 -0.0058 -6.3 -1.5999999999999996 3.49 3064.4 nuORFdb_human ENST00000330736.9_1_16:89383456-89383540:- ENST00000330736.9_1_16:89383456-89383540:-|ENST00000378330.6_1_16:89383456-89484700:-|ENST00000562816.5_1_16:89383456-89484700:- ANKRD11 5' uORF 5' uORF GN=ANKRD11 5' uORF E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep4_Slot2-5_1_3522 5mio-rep1 E_20221201_NO30_400nL_HLAc1_5e6_directIP_titration_rep1_Slot1-37_1_3502.11298.587.1 1 1 1 8.95 4.99 2.13 3.96 70.1 6 0 0 2510.0 Hydrogen 20 (Q) 9 TVDDEAPAV (-) 9 CGTSPGAAPV T V/D\D E|A|P\A\V 55.6 5 5 3 2 0 unbiased 39.028713 1.374271 916.4198 916.426 -0.0058 -6.3 0.7000000000000002 3.49 3064.4 nuORFdb_human ENST00000330736.9_1_16:89383456-89383540:- ENST00000330736.9_1_16:89383456-89383540:-|ENST00000378330.6_1_16:89383456-89484700:-|ENST00000562816.5_1_16:89383456-89484700:- ANKRD11 5' uORF 5' uORF GN=ANKRD11 5' uORF E_20221201_NO30_400nL_HLAc1_5e6_directIP_titration_rep1_Slot1-37_1_3502 SCP E_20221201_NO30_400nL_HLAc1_4e7_PDAC_Slot2-6_1_3524.7350.1452.2 2 1 1 9.61 7.37 4.07 2.24 50.5 6 1 1 36100.0 Hydrogen 3 (W) 8 TVEKQCFL (-) 8 TnIKmKCL T V/E/K|Q\C\F\L 75.0 6 4 3 1 0 unbiased Carbamidomethylation C:Carbamidomethylation, 25.395607 0.843844 512.7591 1024.513 -0.0025 -2.4 0.0 5.66 1341.6 nuORFdb_human ENST00000585291.5_2_17:46128163-46128490:+ ENST00000585291.5_2_17:46128163-46128490:+ NFE2L1 5' Overlap uORF 5' Overlap uORF GN=NFE2L1 5' Overlap uORF E_20221201_NO30_400nL_HLAc1_4e7_PDAC_Slot2-6_1_3524 40mio-rep2 E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep2_Slot2-5_1_3520.9921.1481.2 2 1 1 10.03 2.97 6.75 7.06 68.3 8 1 1 6860.0 Hydrogen 2 (M) 10 TVKSTVGDGW (W) 10 TVSKSIGPTC T/V/K|S\T/V\G D\G\W 80.0 8 5 4 1 0 unbiased 34.27248 0.825746 525.2645 1049.526 -0.0055 -5.2 -0.2999999999999998 5.5 2879.2 nuORFdb_human ENST00000457448.1_1_2:68023725-68051081:- ENST00000457448.1_1_2:68023725-68051081:- LINC01812 lincRNA lncRNA GN=LINC01812 lincRNA E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep2_Slot2-5_1_3520 40mio-rep4 E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep4_Slot2-5_1_3522.9928.1476.2 2 2 1 9.43 9.43 9.43 62.6 8 1 1 13300.0 Hydrogen 2 (M) 10 TVKSTVGDGW (W) 10 T V/K/S\T/V|G\D|G\W 80.0 8 5 5 2 0 unbiased 34.288025 0.828326 525.2627 1049.526 -0.0075 -7.1 -2.3999999999999995 5.5 2879.2 nuORFdb_human ENST00000457448.1_1_2:68023725-68051081:- ENST00000457448.1_1_2:68023725-68051081:- LINC01812 lincRNA lncRNA GN=LINC01812 lincRNA E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep4_Slot2-5_1_3522 SCP E_20221201_NO30_400nL_HLAc1_4e7_PDAC_Slot2-6_1_3524.16095.392.1 1 4 1 14.1 6.95 9.86 7.15 85.4 6 0 0 54500.0 Hydrogen 35 (G) 9 TVSAVCQIY (L) 9 LSLGSLCQY T V/S A|V|C|Q\I\Y 55.6 5 5 4 3 0 dual b/y bias Carbamidomethylation C:Carbamidomethylation, 55.604424 1.490154 1040.5068 1040.508 0.0019 1.8 4.2 5.19 5867.8 nuORFdb_human ENST00000281172.9_1_12:15822752-15835827:- ENST00000281172.9_1_12:15822752-15835827:- EPS8 Out-of-Frame Out-of-Frame GN=EPS8 Out-of-Frame E_20221201_NO30_400nL_HLAc1_4e7_PDAC_Slot2-6_1_3524 Exploris_FAIMS VH20221207_MKP_FAIMS2022E_HLAc1_4e7_PDAC.41090.41090.2 2 1 1 9.89 5.57 4.59 4.32 60.2 6 0 0 137000000.0 Hydrogen 35 (G) 9 TVSAVCQIY (L) 9 VQCLSGTLY T V\S A\V\C-Q/I/Y 55.6 5 3 2 0 1 unbiased Carbamidomethylation C:Carbamidomethylation, 0.967 77.2 75.73 120.0 -50.0 520.7563 1040.508 -0.0027 -2.6 0.0 5.19 5867.8 nuORFdb_human ENST00000281172.9_1_12:15822752-15835827:- ENST00000281172.9_1_12:15822752-15835827:- EPS8 Out-of-Frame Out-of-Frame GN=EPS8 Out-of-Frame VH20221207_MKP_FAIMS2022E_HLAc1_4e7_PDAC SCP E_20221201_NO30_400nL_HLAc1_4e7_PDAC_Slot2-6_1_3524.22706.537.1 1 2 1 10.01 5.74 3.59 4.27 73.2 6 0 0 132000.0 Hydrogen 11 (L) 9 TVSGVSLTF (H) 9 ISEKVSTF T V S/G\V|S\L\T\F 66.7 6 5 2 1 0 b-ion bias 78.444229 1.404021 910.4871 910.488 -0.001 -1.1 1.2999999999999998 5.18 3424.9 nuORFdb_human ENST00000288344.14_1_21:40714727-40714814:- ENST00000288344.14_1_21:40714727-40714814:- HMGN1 3' dORF 3' dORF GN=HMGN1 3' dORF E_20221201_NO30_400nL_HLAc1_4e7_PDAC_Slot2-6_1_3524 SCP E_20221201_NO30_400nL_HLAc1_4e7_PDAC_Slot2-6_1_3524.13033.478.1 1 2 1 12.09 4.37 9.21 7.72 83.6 6 0 0 10500.0 Hydrogen 15 (L) 9 TVSPMTNEM (-) 9 SISDNNAASM T V/S/P M|T|N\E\M 55.6 5 4 4 2 0 unbiased 45.026478 1.439187 1009.4259 1009.433 -0.0029 -2.9 -0.5 4.0 2650.2 nuORFdb_human ENST00000211314.4_1_6:52546628-52546700:+ ENST00000211314.4_1_6:52546628-52546700:+ TMEM14A Out-of-Frame Out-of-Frame GN=TMEM14A Out-of-Frame E_20221201_NO30_400nL_HLAc1_4e7_PDAC_Slot2-6_1_3524 SCP E_20221201_NO30_400nL_HLAc1_4e7_PDAC_Slot2-6_1_3524.10086.434.1 1 1 1 11.34 1.34 11.34 10.0 73.8 7 0 0 3540.0 Hydrogen 168 (S) 9 TVYADGCSY (A) 9 SLYQCDYS T V/Y/A|D|G|C\S\Y 77.8 7 5 5 3 0 dual b/y bias Carbamidomethylation C:Carbamidomethylation, 34.845459 1.464939 1035.4086 1035.409 0.0012 1.2 3.5999999999999996 3.8 29080.6 nuORFdb_human ENST00000552916.5_1_12:56965569-56983042:+ ENST00000552916.5_1_12:56965569-56983042:+ RBMS2 3' Overlap dORF 3' Overlap dORF GN=RBMS2 3' Overlap dORF E_20221201_NO30_400nL_HLAc1_4e7_PDAC_Slot2-6_1_3524 SCP E_20221201_NO30_400nL_HLAc1_4e7_PDAC_Slot2-6_1_3524.29717.76.1 1 1 1 11.16 5.74 4.27 5.42 85.9 6 0 0 4250.0 Hydrogen 30 (E) 10 TYLDPTYILF (L) 10 LYnTLLPYTF T Y L/D|P\T Y\I\L\F 50.0 5 5 2 1 0 b-ion bias 102.670677 1.67509 1245.6406 1245.64 -0.0002 -0.1 2.3 3.8 10257.6 nuORFdb_human T042136_10:54251512-54301824:+ T042136_10:54251512-54301824:+ lincRNA lncRNA GN=nan lincRNA E_20221201_NO30_400nL_HLAc1_4e7_PDAC_Slot2-6_1_3524 SCP E_20221201_NO30_400nL_HLAc1_4e7_PDAC_Slot2-6_1_3524.29292.1238.2 2 1 1 12.91 0.58 12.91 12.33 69.3 10 0 0 8000.0 Hydrogen 35 (F) 11 TYLNFFHISTF (F) 11 TYINGLLTGYSST T/Y/L|N|F|F/H\I|S/T\F 100.0 11 6 8 4 0 dual b/y bias 101.202583 0.977635 695.3438 1389.684 -0.003 -2.2 0.19999999999999973 6.4 8558.8 nuORFdb_human ENST00000361384.6_1_10:115613538-115613757:- ENST00000361384.6_1_10:115613538-115613757:- DCLRE1A 5' uORF 5' uORF GN=DCLRE1A 5' uORF E_20221201_NO30_400nL_HLAc1_4e7_PDAC_Slot2-6_1_3524 Exploris AL20221201_MKP_PDAC_01.22085.22085.3 3 2 1 11.64 7.25 5.4 4.38 83.5 7 2 2 1160000000.0 Hydrogen 39 (A) 9 TYPRSLCRF (F) 9 YTPITNDKFT T/Y|P/R/S L/C/R/F 77.8 7 1 7 1 0 y-ion bias Carbamidomethylation C:Carbamidomethylation, 0.998 92.7 37.55 1.92 0.0 400.5382 1199.599 0.0011 0.9 0.09999999999999998 10.83 10975.6 nuORFdb_human ENST00000549253.5_1_18:3449645-3457414:+ ENST00000549253.5_1_18:3449645-3457414:+|ENST00000551541.5_1_18:3451752-3457414:+|ENST00000345133.9_1_18:3451757-3457414:+ TGIF1 5' Overlap uORF 5' Overlap uORF GN=TGIF1 5' Overlap uORF AL20221201_MKP_PDAC_01 SCP E_20221201_NO30_400nL_HLAc1_4e7_PDAC_Slot2-6_1_3524.10111.1286.2 2 1 1 9.5 5.6 3.18 3.9 81.0 5 2 2 50300.0 Hydrogen 39 (A) 9 TYPRSLCRF (F) 9 FYQRSPRmV T/Y|P R/S L\C R\F 33.3 3 3 3 1 0 unbiased Carbamidomethylation C:Carbamidomethylation, 34.931786 0.947581 600.303 1199.599 -0.0003 -0.2 2.1999999999999997 10.83 10975.6 nuORFdb_human ENST00000549253.5_1_18:3449645-3457414:+ ENST00000549253.5_1_18:3449645-3457414:+|ENST00000551541.5_1_18:3451752-3457414:+|ENST00000345133.9_1_18:3451757-3457414:+ TGIF1 5' Overlap uORF 5' Overlap uORF GN=TGIF1 5' Overlap uORF E_20221201_NO30_400nL_HLAc1_4e7_PDAC_Slot2-6_1_3524 Exploris_FAIMS VH20221207_MKP_FAIMS2022E_HLAc1_4e7_PDAC.28031.28307.3 3 1 1 11.57 7.54 5.18 4.03 80.9 7 2 2 104000000.0 Hydrogen 39 (A) 9 TYPRSLCRF (F) 9 YTPITNDKFT T/Y|P/R/S/L/C-R F 77.8 7 1 6 1 1 y-ion bias Carbamidomethylation C:Carbamidomethylation, 1.0 99.4 56.02 11.52 -50.0 400.537 1199.599 -0.0025 -2.1 0.5 10.83 10975.6 nuORFdb_human ENST00000549253.5_1_18:3449645-3457414:+ ENST00000549253.5_1_18:3449645-3457414:+|ENST00000551541.5_1_18:3451752-3457414:+|ENST00000345133.9_1_18:3451757-3457414:+ TGIF1 5' Overlap uORF 5' Overlap uORF GN=TGIF1 5' Overlap uORF VH20221207_MKP_FAIMS2022E_HLAc1_4e7_PDAC Eva-50mio-3fr AL20210827_EV_HLA_PDAC_PANFRO413_2D_50e6_W632_classI_fxn3.24039.24039.2 2 2 2 13.24 7.84 6.14 5.4 83.9 7 0 0 1340000000.0 Hydrogen 708 (K) 9 TYQDIQNTI (K) 9 LQDQNTYTL T Y\Q\D\I|Q-N/T/I 77.8 7 4 3 1 1 unbiased 0.97 100.0 44.72 100.0 0.0 548.2702 1095.532 0.0014 1.3 0.8 3.8 213870.4 nuORFdb_human ENST00000617998.4_2_17:7388053-7417318:+ ENST00000617998.4_2_17:7388053-7417318:+ POLR2A 5' Overlap uORF 5' Overlap uORF GN=POLR2A 5' Overlap uORF AL20210827_EV_HLA_PDAC_PANFRO413_2D_50e6_W632_classI_fxn3 Exploris AL20221201_MKP_PDAC_01.32848.32983.2 2 1 1 13.46 8.09 6.48 5.37 89.0 7 0 0 3300000000.0 Hydrogen 708 (K) 9 TYQDIQNTI (K) 9 LQDQNTYTL T Y\Q\D\I\Q-N/T/I 77.8 7 4 2 0 1 b-ion bias 0.992 99.4 52.12 0.73 0.0 548.2698 1095.532 0.0006 0.5 -0.30000000000000004 3.8 213870.4 nuORFdb_human ENST00000617998.4_2_17:7388053-7417318:+ ENST00000617998.4_2_17:7388053-7417318:+ POLR2A 5' Overlap uORF 5' Overlap uORF GN=POLR2A 5' Overlap uORF AL20221201_MKP_PDAC_01 SCP E_20221201_NO30_400nL_HLAc1_4e7_PDAC_Slot2-6_1_3524.15703.297.1 1 10 1 16.79 7.9 10.19 8.89 94.1 7 0 0 480000.0 Hydrogen 708 (K) 9 TYQDIQNTI (K) 9 TYLPInmNL T Y/Q|D|I|Q\N\T\I 77.8 7 6 4 3 0 dual b/y bias 54.250099 1.546353 1095.5311 1095.532 -0.0017 -1.5 0.8999999999999999 3.8 213870.4 nuORFdb_human ENST00000617998.4_2_17:7388053-7417318:+ ENST00000617998.4_2_17:7388053-7417318:+ POLR2A 5' Overlap uORF 5' Overlap uORF GN=POLR2A 5' Overlap uORF E_20221201_NO30_400nL_HLAc1_4e7_PDAC_Slot2-6_1_3524 Exploris_FAIMS VH20221207_MKP_FAIMS2022E_HLAc1_4e7_PDAC.39787.39915.2 2 1 1 10.72 6.9 5.03 3.82 72.7 7 0 0 737000000.0 Hydrogen 708 (K) 9 TYQDIQNTI (K) 9 TNQIYTNEI T Y\Q\D\I\Q-N/T/I 77.8 7 4 2 0 1 b-ion bias 0.989 77.8 73.45 0.5 -50.0 548.268 1095.532 -0.003 -2.7 -0.10000000000000009 3.8 213870.4 nuORFdb_human ENST00000617998.4_2_17:7388053-7417318:+ ENST00000617998.4_2_17:7388053-7417318:+ POLR2A 5' Overlap uORF 5' Overlap uORF GN=POLR2A 5' Overlap uORF VH20221207_MKP_FAIMS2022E_HLAc1_4e7_PDAC 10mio-rep1 E_20221201_NO30_400nL_HLAc1_1e7_directIP_titration_rep1_Slot2-3_1_3512.6980.1677.3 3 3 1 10.2 8.83 3.47 1.38 79.7 6 1 2 12500.0 Hydrogen 356 (S) 9 TYSSQIRKL (R) 9 TYSKAVSGGLL T/Y|S/S/Q/I R-K L 55.6 5 1 5 1 1 y-ion bias 24.122046 0.7013 365.8748 1095.616 -0.0052 -4.8 2.9000000000000004 9.99 51147.2 nuORFdb_human ENST00000469130.5_1_1:201751782-201763782:+ ENST00000469130.5_1_1:201751782-201763782:+|ENST00000477118.2_3_1:201756811-201759996:+ NAV1 3' Overlap dORF 3' Overlap dORF GN=NAV1 3' Overlap dORF E_20221201_NO30_400nL_HLAc1_1e7_directIP_titration_rep1_Slot2-3_1_3512 10mio-rep2 E_20221201_NO30_400nL_HLAc1_1e7_directIP_titration_rep2_Slot2-3_1_3513.6915.1685.3 3 3 1 9.11 5.5 4.02 3.61 80.1 7 1 2 13900.0 Hydrogen 356 (S) 9 TYSSQIRKL (R) 9 TYLLSTRIE T/Y/S/S/Q/I/R K-L 77.8 7 0 6 0 1 y-ion bias 23.895771 0.696188 365.8757 1095.616 -0.0022 -2.0 4.7 9.99 51147.2 nuORFdb_human ENST00000469130.5_1_1:201751782-201763782:+ ENST00000469130.5_1_1:201751782-201763782:+|ENST00000477118.2_3_1:201756811-201759996:+ NAV1 3' Overlap dORF 3' Overlap dORF GN=NAV1 3' Overlap dORF E_20221201_NO30_400nL_HLAc1_1e7_directIP_titration_rep2_Slot2-3_1_3513 10mio-rep3 E_20221201_NO30_400nL_HLAc1_1e7_directIP_titration_rep3_Slot2-3_1_3514.6947.1676.3 3 3 1 11.63 7.65 4.67 3.98 83.5 7 1 2 17800.0 Hydrogen 356 (S) 9 TYSSQIRKL (R) 9 YTSQTVKRL T/Y|S/S/Q/I/R K-L 77.8 7 1 6 1 1 y-ion bias 24.006847 0.70182 365.8751 1095.616 -0.0052 -4.8 1.5 9.99 51147.2 nuORFdb_human ENST00000469130.5_1_1:201751782-201763782:+ ENST00000469130.5_1_1:201751782-201763782:+|ENST00000477118.2_3_1:201756811-201759996:+ NAV1 3' Overlap dORF 3' Overlap dORF GN=NAV1 3' Overlap dORF E_20221201_NO30_400nL_HLAc1_1e7_directIP_titration_rep3_Slot2-3_1_3514 20mio-rep1 E_20221201_NO30_400nL_HLAc1_2e7_directIP_titration_rep1_Slot2-4_1_3515.6866.1401.2 2 3 1 13.27 8.89 5.74 4.38 79.0 7 1 2 41400.0 Hydrogen 356 (S) 9 TYSSQIRKL (R) 9 YTSQTVKRL T/Y|S|S/Q I/R/K\L 77.8 7 3 6 2 0 y-ion bias 23.721165 0.875758 548.3079 1095.616 -0.007 -6.4 -0.20000000000000018 9.99 51147.2 nuORFdb_human ENST00000469130.5_1_1:201751782-201763782:+ ENST00000469130.5_1_1:201751782-201763782:+|ENST00000477118.2_3_1:201756811-201759996:+ NAV1 3' Overlap dORF 3' Overlap dORF GN=NAV1 3' Overlap dORF E_20221201_NO30_400nL_HLAc1_2e7_directIP_titration_rep1_Slot2-4_1_3515 20mio-rep2 E_20221201_NO30_400nL_HLAc1_2e7_directIP_titration_rep3_Slot2-4_1_3518.7022.1671.3 3 5 1 10.82 6.45 4.43 4.36 81.3 6 1 2 47900.0 Hydrogen 356 (S) 9 TYSSQIRKL (R) 9 TYSKAVSGGLL T/Y|S/S Q|I/R K-L 55.6 5 2 5 2 1 y-ion bias 24.261663 0.705096 365.8736 1095.616 -0.0082 -7.5 -1.2000000000000002 9.99 51147.2 nuORFdb_human ENST00000469130.5_1_1:201751782-201763782:+ ENST00000469130.5_1_1:201751782-201763782:+|ENST00000477118.2_3_1:201756811-201759996:+ NAV1 3' Overlap dORF 3' Overlap dORF GN=NAV1 3' Overlap dORF E_20221201_NO30_400nL_HLAc1_2e7_directIP_titration_rep3_Slot2-4_1_3518 20mio-rep3 E_20221201_NO30_400nL_HLAc1_2e7_directIP_titration_rep4_Slot2-4_1_3519.6902.1678.3 3 5 1 11.19 7.6 2.15 3.59 85.1 6 1 2 39300.0 Hydrogen 356 (S) 9 TYSSQIRKL (R) 9 TYSKAVSGGLL T/Y|S/S/Q/I R-K L 55.6 5 1 5 1 1 y-ion bias 23.844715 0.700926 365.8737 1095.616 -0.0082 -7.5 -1.7000000000000002 9.99 51147.2 nuORFdb_human ENST00000469130.5_1_1:201751782-201763782:+ ENST00000469130.5_1_1:201751782-201763782:+|ENST00000477118.2_3_1:201756811-201759996:+ NAV1 3' Overlap dORF 3' Overlap dORF GN=NAV1 3' Overlap dORF E_20221201_NO30_400nL_HLAc1_2e7_directIP_titration_rep4_Slot2-4_1_3519 40mio-rep1 E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep1_Slot1-04_1_3496.7130.1372.2 2 6 1 17.09 10.68 8.95 6.41 87.9 7 1 2 176000.0 Hydrogen 356 (S) 9 TYSSQIRKL (R) 9 YTSQTVKRL T/Y|S|S|Q\I R\K\L 77.8 7 6 4 3 0 dual b/y bias 24.636721 0.893944 548.3098 1095.616 -0.003 -2.7 0.5 9.99 51147.2 nuORFdb_human ENST00000469130.5_1_1:201751782-201763782:+ ENST00000469130.5_1_1:201751782-201763782:+|ENST00000477118.2_3_1:201756811-201759996:+ NAV1 3' Overlap dORF 3' Overlap dORF GN=NAV1 3' Overlap dORF E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep1_Slot1-04_1_3496 40mio-rep2 E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep2_Slot2-5_1_3520.6866.1388.2 2 6 1 16.95 11.07 8.24 5.88 84.9 8 1 2 131000.0 Hydrogen 356 (S) 9 TYSSQIRKL (R) 9 TYSKAVSGGLL T/Y|S|S/Q\I\R|K\L 100.0 9 6 5 3 0 dual b/y bias 23.724939 0.883734 548.3077 1095.616 -0.007 -6.4 -1.5 9.99 51147.2 nuORFdb_human ENST00000469130.5_1_1:201751782-201763782:+ ENST00000469130.5_1_1:201751782-201763782:+|ENST00000477118.2_3_1:201756811-201759996:+ NAV1 3' Overlap dORF 3' Overlap dORF GN=NAV1 3' Overlap dORF E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep2_Slot2-5_1_3520 40mio-rep3 E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep3_Slot2-5_1_3521.6876.1669.3 3 5 1 14.52 10.11 4.41 4.41 88.9 7 1 2 70800.0 Hydrogen 356 (S) 9 TYSSQIRKL (R) 9 TYLDTRTLL T/Y|S/S/Q/I R-K\L 77.8 7 2 5 1 1 y-ion bias 23.75577 0.706418 365.874 1095.616 -0.0082 -7.5 -2.9000000000000004 9.99 51147.2 nuORFdb_human ENST00000469130.5_1_1:201751782-201763782:+ ENST00000469130.5_1_1:201751782-201763782:+|ENST00000477118.2_3_1:201756811-201759996:+ NAV1 3' Overlap dORF 3' Overlap dORF GN=NAV1 3' Overlap dORF E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep3_Slot2-5_1_3521 40mio-rep4 E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep4_Slot2-5_1_3522.6890.1670.3 3 3 1 13.45 8.54 3.41 4.91 86.0 7 1 2 141000.0 Hydrogen 356 (S) 9 TYSSQIRKL (R) 9 TYSKAVSGGLL T/Y|S/S/Q/I R-K\L 77.8 7 2 5 1 1 y-ion bias 23.799843 0.705655 365.875 1095.616 -0.0052 -4.8 -0.09999999999999964 9.99 51147.2 nuORFdb_human ENST00000469130.5_1_1:201751782-201763782:+ ENST00000469130.5_1_1:201751782-201763782:+|ENST00000477118.2_3_1:201756811-201759996:+ NAV1 3' Overlap dORF 3' Overlap dORF GN=NAV1 3' Overlap dORF E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep4_Slot2-5_1_3522 40mio-rep1 E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep1_Slot1-04_1_3496.27636.1391.2 2 3 1 15.11 10.06 6.65 5.05 91.1 5 0 0 14400.0 Hydrogen 2 (M) 11 VAACTLGSLVW (C) 11 LACALSSLGVW V A A C T L|G\S\L|V\W 45.5 5 5 2 2 0 b-ion bias Carbamidomethylation C:Carbamidomethylation, 95.474731 0.881808 588.8057 1176.608 -0.0034 -2.9 0.30000000000000027 5.49 3432.0 smORF_v4|nuORFdb_human PIK3R2_19:18266291-18266383:+ PIK3R2_19:18266291-18266383:+|ENST00000222254.12_1_19:18263987-18266380:+ PIK3R2 uORF 19:18266291-18266383:+ 19 PIK3R2 5' uORF 5' uORF GN=PIK3R2 E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep1_Slot1-04_1_3496 40mio-rep2 E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep2_Slot2-5_1_3520.27472.1391.2 2 1 1 15.46 10.77 6.6 4.7 89.2 7 0 0 6550.0 Hydrogen 2 (M) 11 VAACTLGSLVW (C) 11 GFVLGSTPREGG V A A C\T\L|G\S|L|V\W 63.6 7 7 3 3 0 b-ion bias Carbamidomethylation C:Carbamidomethylation, 94.903053 0.882297 588.8048 1176.608 -0.0054 -4.6 0.3000000000000007 5.49 3432.0 smORF_v4|nuORFdb_human PIK3R2_19:18266291-18266383:+ PIK3R2_19:18266291-18266383:+|ENST00000222254.12_1_19:18263987-18266380:+ PIK3R2 uORF 19:18266291-18266383:+ 19 PIK3R2 5' uORF 5' uORF GN=PIK3R2 E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep2_Slot2-5_1_3520 40mio-rep3 E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep3_Slot2-5_1_3521.27482.1404.2 2 1 1 12.73 8.6 3.97 4.13 85.3 5 0 0 9120.0 Hydrogen 2 (M) 11 VAACTLGSLVW (C) 11 GFVLGSTPREGG V A A C T L|G\S|L|V\W 45.5 5 5 3 3 0 unbiased Carbamidomethylation C:Carbamidomethylation, 94.930122 0.873577 588.8042 1176.608 -0.0074 -6.3 -1.7000000000000002 5.49 3432.0 smORF_v4|nuORFdb_human PIK3R2_19:18266291-18266383:+ PIK3R2_19:18266291-18266383:+|ENST00000222254.12_1_19:18263987-18266380:+ PIK3R2 uORF 19:18266291-18266383:+ 19 PIK3R2 5' uORF 5' uORF GN=PIK3R2 E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep3_Slot2-5_1_3521 40mio-rep4 E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep4_Slot2-5_1_3522.27488.1400.2 2 1 1 14.32 9.57 6.84 4.75 89.5 6 0 0 4800.0 Hydrogen 2 (M) 11 VAACTLGSLVW (C) 11 LACALSSLGVW V A A C\T L|G\S\L|V\W 45.5 5 6 2 2 0 b-ion bias Carbamidomethylation C:Carbamidomethylation, 94.943489 0.876122 588.8017 1176.608 -0.0114 -9.7 -4.999999999999999 5.49 3432.0 smORF_v4|nuORFdb_human PIK3R2_19:18266291-18266383:+ PIK3R2_19:18266291-18266383:+|ENST00000222254.12_1_19:18263987-18266380:+ PIK3R2 uORF 19:18266291-18266383:+ 19 PIK3R2 5' uORF 5' uORF GN=PIK3R2 E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep4_Slot2-5_1_3522 20mio-rep1 E_20221201_NO30_400nL_HLAc1_2e7_directIP_titration_rep1_Slot2-4_1_3515.25950.1373.2 2 1 1 9.3 6.2 2.53 3.09 71.5 6 0 0 2760.0 C5c Hydrogen 2 (M) 11 VAAcTLGSLVW (C) 11 IScARREVW V A/A c T/L\G\S L|V\W 36.4 4 4 3 1 0 unbiased c:Cysteinylation 89.610123 0.893476 619.7954 1176.608 61.9746 -6.5 -0.2999999999999998 5.49 3432.0 smORF_v4|nuORFdb_human PIK3R2_19:18266291-18266383:+ PIK3R2_19:18266291-18266383:+|ENST00000222254.12_1_19:18263987-18266380:+ PIK3R2 uORF 19:18266291-18266383:+ 19 PIK3R2 5' uORF 5' uORF GN=PIK3R2 E_20221201_NO30_400nL_HLAc1_2e7_directIP_titration_rep1_Slot2-4_1_3515 20mio-rep3 E_20221201_NO30_400nL_HLAc1_2e7_directIP_titration_rep4_Slot2-4_1_3519.26016.1371.2 2 1 1 7.88 5.72 2.29 2.16 68.8 5 0 0 3140.0 C5c Hydrogen 2 (M) 11 VAAcTLGSLVW (C) 11 LAcALSSLGVW V A/A c T L\G S\L|V\W 27.3 3 4 2 1 0 b-ion bias c:Cysteinylation 89.853508 0.894386 619.7954 1176.608 61.9746 -6.5 -0.7000000000000002 5.49 3432.0 smORF_v4|nuORFdb_human PIK3R2_19:18266291-18266383:+ PIK3R2_19:18266291-18266383:+|ENST00000222254.12_1_19:18263987-18266380:+ PIK3R2 uORF 19:18266291-18266383:+ 19 PIK3R2 5' uORF 5' uORF GN=PIK3R2 E_20221201_NO30_400nL_HLAc1_2e7_directIP_titration_rep4_Slot2-4_1_3519 40mio-rep1 E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep1_Slot1-04_1_3496.26207.1336.2 2 1 1 16.07 10.99 4.58 5.08 89.2 7 0 0 11500.0 C5c Hydrogen 2 (M) 11 VAAcTLGSLVW (C) 11 AAncTKILVW V A A c/T\L|G\S|L|V|W 63.6 7 6 5 4 0 dual b/y bias c:Cysteinylation 90.536209 0.916685 619.7958 1176.608 61.9766 -4.9 -1.7000000000000002 5.49 3432.0 smORF_v4|nuORFdb_human PIK3R2_19:18266291-18266383:+ PIK3R2_19:18266291-18266383:+|ENST00000222254.12_1_19:18263987-18266380:+ PIK3R2 uORF 19:18266291-18266383:+ 19 PIK3R2 5' uORF 5' uORF GN=PIK3R2 E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep1_Slot1-04_1_3496 40mio-rep2 E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep2_Slot2-5_1_3520.25975.1352.2 2 1 1 11.48 6.73 4.22 4.75 77.9 7 0 0 7090.0 C5c Hydrogen 2 (M) 11 VAAcTLGSLVW (C) 11 LAcALSSLGVW V/A A c/T L\G\S\L|V\W 54.5 6 5 3 1 0 unbiased c:Cysteinylation 89.730766 0.906294 619.7951 1176.608 61.9746 -6.5 -1.5999999999999996 5.49 3432.0 smORF_v4|nuORFdb_human PIK3R2_19:18266291-18266383:+ PIK3R2_19:18266291-18266383:+|ENST00000222254.12_1_19:18263987-18266380:+ PIK3R2 uORF 19:18266291-18266383:+ 19 PIK3R2 5' uORF 5' uORF GN=PIK3R2 E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep2_Slot2-5_1_3520 40mio-rep3 E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep3_Slot2-5_1_3521.25978.1349.2 2 1 1 8.36 6.23 3.06 2.13 65.2 5 0 0 1880.0 C5c Hydrogen 2 (M) 11 VAAcTLGSLVW (C) 11 LAcALSSLGVW V A A c T\L|G-S\L|V\W 54.5 6 5 2 2 1 b-ion bias c:Cysteinylation 89.736778 0.908253 619.7987 1176.608 61.9826 -0.1 4.5 5.49 3432.0 smORF_v4|nuORFdb_human PIK3R2_19:18266291-18266383:+ PIK3R2_19:18266291-18266383:+|ENST00000222254.12_1_19:18263987-18266380:+ PIK3R2 uORF 19:18266291-18266383:+ 19 PIK3R2 5' uORF 5' uORF GN=PIK3R2 E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep3_Slot2-5_1_3521 40mio-rep4 E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep4_Slot2-5_1_3522.26005.1343.2 2 2 1 11.82 7.86 4.54 3.96 77.0 6 0 0 8390.0 C5c Hydrogen 2 (M) 11 VAAcTLGSLVW (C) 11 LAcALSSLGVW V A A c T/L|G\S\L|V\W 54.5 6 5 3 2 0 unbiased c:Cysteinylation 89.820831 0.912278 619.7961 1176.608 61.9766 -4.9 -0.20000000000000018 5.49 3432.0 smORF_v4|nuORFdb_human PIK3R2_19:18266291-18266383:+ PIK3R2_19:18266291-18266383:+|ENST00000222254.12_1_19:18263987-18266380:+ PIK3R2 uORF 19:18266291-18266383:+ 19 PIK3R2 5' uORF 5' uORF GN=PIK3R2 E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep4_Slot2-5_1_3522 20mio-rep2 E_20221201_NO30_400nL_HLAc1_2e7_directIP_titration_rep3_Slot2-4_1_3518.13894.1531.2 2 1 1 8.34 3.66 3.83 4.68 67.6 7 1 1 4360.0 Hydrogen 12 (A) 9 VAATAARIW (A) 9 VAFLRQQP V/A/A/T|A/A R\I\W 77.8 7 3 5 1 0 unbiased 47.989651 0.79372 479.7728 958.547 -0.0082 -8.5 -2.2 9.72 53373.6 nuORFdb_human ENST00000586720.5_1_17:76967660-76975958:- ENST00000586720.5_1_17:76967660-76975958:-|ENST00000589906.5_2_17:76970879-76975958:-|ENST00000262776.7_1_17:76972225-76976165:-|ENST00000587310.5_1_17:76973066-76975958:-|ENST00000588899.1_1_17:76973220-76975958:- LGALS3BP 3' Overlap dORF 3' Overlap dORF GN=LGALS3BP 3' Overlap dORF E_20221201_NO30_400nL_HLAc1_2e7_directIP_titration_rep3_Slot2-4_1_3518 40mio-rep1 E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep1_Slot1-04_1_3496.14086.1503.2 2 1 1 12.77 5.97 5.5 6.8 87.7 7 1 1 9030.0 Hydrogen 12 (A) 9 VAATAARIW (A) 9 VATAGLRDR V/A/A|T|A|A\R I\W 77.8 7 5 5 3 0 dual b/y bias 48.657543 0.811359 479.7747 958.547 -0.0042 -4.3 -1.0999999999999996 9.72 53373.6 nuORFdb_human ENST00000586720.5_1_17:76967660-76975958:- ENST00000586720.5_1_17:76967660-76975958:-|ENST00000589906.5_2_17:76970879-76975958:-|ENST00000262776.7_1_17:76972225-76976165:-|ENST00000587310.5_1_17:76973066-76975958:-|ENST00000588899.1_1_17:76973220-76975958:- LGALS3BP 3' Overlap dORF 3' Overlap dORF GN=LGALS3BP 3' Overlap dORF E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep1_Slot1-04_1_3496 40mio-rep2 E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep2_Slot2-5_1_3520.13775.1516.2 2 1 1 13.93 6.98 4.5 6.95 88.7 7 1 1 11500.0 Hydrogen 12 (A) 9 VAATAARIW (A) 9 LQTRDRIG V/A/A|T|A|A R\I\W 77.8 7 5 5 3 0 dual b/y bias 47.584976 0.803636 479.7752 958.547 -0.0042 -4.3 0.6000000000000005 9.72 53373.6 nuORFdb_human ENST00000586720.5_1_17:76967660-76975958:- ENST00000586720.5_1_17:76967660-76975958:-|ENST00000589906.5_2_17:76970879-76975958:-|ENST00000262776.7_1_17:76972225-76976165:-|ENST00000587310.5_1_17:76973066-76975958:-|ENST00000588899.1_1_17:76973220-76975958:- LGALS3BP 3' Overlap dORF 3' Overlap dORF GN=LGALS3BP 3' Overlap dORF E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep2_Slot2-5_1_3520 40mio-rep3 E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep3_Slot2-5_1_3521.13778.1530.2 2 2 1 12.74 5.64 4.46 7.1 83.8 7 1 1 16100.0 Hydrogen 12 (A) 9 VAATAARIW (A) 9 GLAVRICAV V/A/A/T|A/A R\I\W 77.8 7 3 5 1 0 unbiased 47.599121 0.794488 479.7744 958.547 -0.0062 -6.4 -1.8000000000000007 9.72 53373.6 nuORFdb_human ENST00000586720.5_1_17:76967660-76975958:- ENST00000586720.5_1_17:76967660-76975958:-|ENST00000589906.5_2_17:76970879-76975958:-|ENST00000262776.7_1_17:76972225-76976165:-|ENST00000587310.5_1_17:76973066-76975958:-|ENST00000588899.1_1_17:76973220-76975958:- LGALS3BP 3' Overlap dORF 3' Overlap dORF GN=LGALS3BP 3' Overlap dORF E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep3_Slot2-5_1_3521 40mio-rep4 E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep4_Slot2-5_1_3522.13771.1513.2 2 2 1 15.51 8.41 7.09 7.1 90.0 8 1 1 10000.0 Hydrogen 12 (A) 9 VAATAARIW (A) 9 GIAKnVWAV V/A/A|T|A|A\R|I\W 100.0 9 6 6 4 0 dual b/y bias 47.558357 0.805077 479.7743 958.547 -0.0062 -6.4 -1.7000000000000002 9.72 53373.6 nuORFdb_human ENST00000586720.5_1_17:76967660-76975958:- ENST00000586720.5_1_17:76967660-76975958:-|ENST00000589906.5_2_17:76970879-76975958:-|ENST00000262776.7_1_17:76972225-76976165:-|ENST00000587310.5_1_17:76973066-76975958:-|ENST00000588899.1_1_17:76973220-76975958:- LGALS3BP 3' Overlap dORF 3' Overlap dORF GN=LGALS3BP 3' Overlap dORF E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep4_Slot2-5_1_3522 40mio-rep4 E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep4_Slot2-5_1_3522.27467.1518.2 2 1 1 8.01 6.09 2.62 1.92 63.3 6 0 0 1980.0 Hydrogen 22 (C) 9 VAELQSGLW (L) 9 LGAEGSLGLW V A/E\L Q-S\G|L\W 55.6 5 4 2 1 1 b-ion bias 94.871017 0.802356 501.7633 1002.525 -0.0067 -6.7 -2.0 4.0 3581.1 nuORFdb_human ENST00000378289.8_1_10:14976421-14976514:- ENST00000378289.8_1_10:14976421-14976514:- DCLRE1C Out-of-Frame Out-of-Frame GN=DCLRE1C Out-of-Frame E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep4_Slot2-5_1_3522 40mio-rep1 E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep1_Slot1-04_1_3496.26520.1431.2 2 3 1 10.7 7.96 5.0 2.74 83.3 6 1 1 23900.0 Hydrogen 3 (S) 9 VAFGFPRVF (-) 9 VAFGPAFALF V/A/F|G|F P/R V\F 55.6 5 3 5 2 0 unbiased 91.616913 0.856692 520.2882 1039.572 -0.0036 -3.5 -0.2999999999999998 9.72 1239.5 nuORFdb_human ENST00000640804.1_1_11:93517530-93517563:+ ENST00000640804.1_1_11:93517530-93517563:+ MED17 5' uORF 5' uORF GN=MED17 5' uORF E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep1_Slot1-04_1_3496 40mio-rep2 E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep2_Slot2-5_1_3520.26321.1442.2 2 1 1 7.91 6.8 3.11 1.11 75.9 5 1 1 5170.0 Hydrogen 3 (S) 9 VAFGFPRVF (-) 9 VAFGPAFALF V/A/F|G|F-P R V F 55.6 5 2 4 2 1 y-ion bias 90.928154 0.850304 520.2873 1039.572 -0.0056 -5.4 -0.5 9.72 1239.5 nuORFdb_human ENST00000640804.1_1_11:93517530-93517563:+ ENST00000640804.1_1_11:93517530-93517563:+ MED17 5' uORF 5' uORF GN=MED17 5' uORF E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep2_Slot2-5_1_3520 40mio-rep1 E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep1_Slot1-04_1_3496.28525.498.1 1 1 1 11.26 8.68 1.11 2.58 83.5 5 0 0 37300.0 Hydrogen 25 (R) 9 VAMLPGLAL (L) 9 AVMIPQLL V A M|L|P G\L\A\L 44.4 4 5 2 2 0 b-ion bias 98.545708 1.426896 884.5249 884.527 -0.0024 -2.7 0.5 5.49 37609.8 nuORFdb_human ENST00000474136.5_1_21:27382178-27543016:- ENST00000474136.5_1_21:27382178-27543016:- APP 5' Overlap uORF 5' Overlap uORF GN=APP 5' Overlap uORF E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep1_Slot1-04_1_3496 40mio-rep3 E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep3_Slot2-5_1_3521.28445.513.1 1 1 1 12.19 10.26 1.68 1.93 85.2 6 0 0 7470.0 Hydrogen 25 (R) 9 VAMLPGLAL (L) 9 AVMIPQLL V A M|L|P\G\L\A\L 66.7 6 6 2 2 0 b-ion bias 98.257614 1.41837 884.5221 884.527 -0.0054 -6.1 -1.5 5.49 37609.8 nuORFdb_human ENST00000474136.5_1_21:27382178-27543016:- ENST00000474136.5_1_21:27382178-27543016:- APP 5' Overlap uORF 5' Overlap uORF GN=APP 5' Overlap uORF E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep3_Slot2-5_1_3521 40mio-rep4 E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep4_Slot2-5_1_3522.28448.511.1 1 1 1 11.56 9.7 3.26 1.86 83.8 6 0 0 19600.0 Hydrogen 25 (R) 9 VAMLPGLAL (L) 9 AVMIPQLL V A M|L|P\G\L\A\L 66.7 6 6 2 2 0 b-ion bias 98.258163 1.419241 884.5241 884.527 -0.0034 -3.8 0.9000000000000004 5.49 37609.8 nuORFdb_human ENST00000474136.5_1_21:27382178-27543016:- ENST00000474136.5_1_21:27382178-27543016:- APP 5' Overlap uORF 5' Overlap uORF GN=APP 5' Overlap uORF E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep4_Slot2-5_1_3522 10mio-rep2 E_20221201_NO30_400nL_HLAc1_1e7_directIP_titration_rep2_Slot2-3_1_3513.25892.514.1 1 2 1 12.67 11.5 3.61 1.17 87.1 6 0 0 5220.0 M27m Hydrogen 25 (R) 9 VAmLPGLAL (L) 9 AVmIPQLL V A/m|L|P G\L\A\L 55.6 5 5 3 2 0 unbiased m:Oxidized methionine 89.426247 1.417355 900.5175 884.527 15.9896 -5.9 0.7999999999999998 5.49 37609.8 nuORFdb_human ENST00000474136.5_1_21:27382178-27543016:- ENST00000474136.5_1_21:27382178-27543016:- APP 5' Overlap uORF 5' Overlap uORF GN=APP 5' Overlap uORF E_20221201_NO30_400nL_HLAc1_1e7_directIP_titration_rep2_Slot2-3_1_3513 20mio-rep1 E_20221201_NO30_400nL_HLAc1_2e7_directIP_titration_rep1_Slot2-4_1_3515.25859.514.1 1 1 1 14.48 12.74 4.3 1.75 90.9 6 0 0 5360.0 M27m Hydrogen 25 (R) 9 VAmLPGLAL (L) 9 AVmIPQLL V A/m|L|P\G L\A\L 55.6 5 5 3 2 0 unbiased m:Oxidized methionine 89.296051 1.417831 900.5188 884.527 15.9916 -3.7 2.5 5.49 37609.8 nuORFdb_human ENST00000474136.5_1_21:27382178-27543016:- ENST00000474136.5_1_21:27382178-27543016:- APP 5' Overlap uORF 5' Overlap uORF GN=APP 5' Overlap uORF E_20221201_NO30_400nL_HLAc1_2e7_directIP_titration_rep1_Slot2-4_1_3515 20mio-rep2 E_20221201_NO30_400nL_HLAc1_2e7_directIP_titration_rep3_Slot2-4_1_3518.25873.508.1 1 1 1 15.35 13.36 4.13 2.0 92.4 7 0 0 2210.0 M27m Hydrogen 25 (R) 9 VAmLPGLAL (L) 9 AVmIPQLL V/A/m|L|P\G L\A\L 77.8 7 5 4 2 0 unbiased m:Oxidized methionine 89.364098 1.421308 900.517 884.527 15.9896 -5.9 0.39999999999999947 5.49 37609.8 nuORFdb_human ENST00000474136.5_1_21:27382178-27543016:- ENST00000474136.5_1_21:27382178-27543016:- APP 5' Overlap uORF 5' Overlap uORF GN=APP 5' Overlap uORF E_20221201_NO30_400nL_HLAc1_2e7_directIP_titration_rep3_Slot2-4_1_3518 20mio-rep3 E_20221201_NO30_400nL_HLAc1_2e7_directIP_titration_rep4_Slot2-4_1_3519.25925.506.1 1 3 1 14.15 11.29 3.73 2.86 91.7 6 0 0 14800.0 M27m Hydrogen 25 (R) 9 VAmLPGLAL (L) 9 AVmIPQLL V A m|L|P\G\L\A\L 66.7 6 6 2 2 0 b-ion bias m:Oxidized methionine 89.536507 1.422296 900.5174 884.527 15.9896 -5.9 -0.10000000000000053 5.49 37609.8 nuORFdb_human ENST00000474136.5_1_21:27382178-27543016:- ENST00000474136.5_1_21:27382178-27543016:- APP 5' Overlap uORF 5' Overlap uORF GN=APP 5' Overlap uORF E_20221201_NO30_400nL_HLAc1_2e7_directIP_titration_rep4_Slot2-4_1_3519 40mio-rep1 E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep1_Slot1-04_1_3496.26173.483.1 1 2 1 12.44 10.72 3.4 1.72 82.1 6 0 0 13400.0 M27m Hydrogen 25 (R) 9 VAmLPGLAL (L) 9 AVmIPQLL V A/m|L|P G\L\A\L 55.6 5 5 3 2 0 unbiased m:Oxidized methionine 90.418854 1.436087 900.5177 884.527 15.9906 -4.8 -1.5999999999999996 5.49 37609.8 nuORFdb_human ENST00000474136.5_1_21:27382178-27543016:- ENST00000474136.5_1_21:27382178-27543016:- APP 5' Overlap uORF 5' Overlap uORF GN=APP 5' Overlap uORF E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep1_Slot1-04_1_3496 40mio-rep3 E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep3_Slot2-5_1_3521.25882.502.1 1 3 1 16.24 13.71 5.78 2.53 91.8 7 0 0 10500.0 M27m Hydrogen 25 (R) 9 VAmLPGLAL (L) 9 AVmIPQLL V A/m|L|P\G\L\A\L 77.8 7 6 3 2 0 b-ion bias m:Oxidized methionine 89.405365 1.424808 900.5185 884.527 15.9916 -3.7 0.8999999999999995 5.49 37609.8 nuORFdb_human ENST00000474136.5_1_21:27382178-27543016:- ENST00000474136.5_1_21:27382178-27543016:- APP 5' Overlap uORF 5' Overlap uORF GN=APP 5' Overlap uORF E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep3_Slot2-5_1_3521 40mio-rep4 E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep4_Slot2-5_1_3522.25988.1515.2 2 3 1 10.66 8.65 -0.06 2.01 78.6 6 0 0 10400.0 M27m Hydrogen 25 (R) 9 VAmLPGLAL (L) 9 IYPGPLGAL V A m\L/P\G|L\A\L 66.7 6 5 2 1 0 b-ion bias m:Oxidized methionine 89.762161 0.803862 450.7617 884.527 15.9893 -6.2 -1.5 5.49 37609.8 nuORFdb_human ENST00000474136.5_1_21:27382178-27543016:- ENST00000474136.5_1_21:27382178-27543016:- APP 5' Overlap uORF 5' Overlap uORF GN=APP 5' Overlap uORF E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep4_Slot2-5_1_3522 10mio-rep2 E_20221201_NO30_400nL_HLAc1_1e7_directIP_titration_rep2_Slot2-3_1_3513.12277.1507.2 2 1 1 9.67 7.1 3.05 2.57 82.5 5 1 1 5650.0 Hydrogen 11 (T) 9 VARSSVVQW (-) 9 VITRQTQW V A/R S S\V\V|Q\W 44.4 4 4 2 1 0 b-ion bias 42.415077 0.808985 516.2811 1031.563 -0.0085 -8.3 -1.6000000000000005 9.72 2108.5 nuORFdb_human ENST00000543258.1_12:9096425-9096485:- ENST00000543258.1_12:9096425-9096485:- M6PR Nonsense Mediated Decay Other GN=M6PR Nonsense Mediated Decay E_20221201_NO30_400nL_HLAc1_1e7_directIP_titration_rep2_Slot2-3_1_3513 20mio-rep1 E_20221201_NO30_400nL_HLAc1_2e7_directIP_titration_rep1_Slot2-4_1_3515.12192.1514.2 2 1 1 10.21 7.13 3.59 3.08 80.5 6 1 1 6330.0 Hydrogen 11 (T) 9 VARSSVVQW (-) 9 KEVSRVQW V A/R/S S/V|V|Q\W 55.6 5 3 5 2 0 unbiased 42.112057 0.804946 516.2818 1031.563 -0.0065 -6.3 -0.09999999999999964 9.72 2108.5 nuORFdb_human ENST00000543258.1_12:9096425-9096485:- ENST00000543258.1_12:9096425-9096485:- M6PR Nonsense Mediated Decay Other GN=M6PR Nonsense Mediated Decay E_20221201_NO30_400nL_HLAc1_2e7_directIP_titration_rep1_Slot2-4_1_3515 20mio-rep3 E_20221201_NO30_400nL_HLAc1_2e7_directIP_titration_rep4_Slot2-4_1_3519.12236.1502.2 2 1 1 11.11 8.51 4.18 2.59 80.6 5 1 1 7190.0 Hydrogen 11 (T) 9 VARSSVVQW (-) 9 KEVSRVQW V A R\S S\V|V|Q|W 44.4 4 5 3 3 0 dual b/y bias 42.268105 0.812214 516.2831 1031.563 -0.0045 -4.4 1.3999999999999995 9.72 2108.5 nuORFdb_human ENST00000543258.1_12:9096425-9096485:- ENST00000543258.1_12:9096425-9096485:- M6PR Nonsense Mediated Decay Other GN=M6PR Nonsense Mediated Decay E_20221201_NO30_400nL_HLAc1_2e7_directIP_titration_rep4_Slot2-4_1_3519 40mio-rep1 E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep1_Slot1-04_1_3496.12498.1490.2 2 1 1 13.02 9.27 3.74 3.75 86.7 5 1 1 42300.0 Hydrogen 11 (T) 9 VARSSVVQW (-) 9 KEVSRVQW V A/R S S\V|V|Q\W 44.4 4 4 3 2 0 unbiased 43.172047 0.820065 516.2832 1031.563 -0.0045 -4.4 -1.2000000000000002 9.72 2108.5 nuORFdb_human ENST00000543258.1_12:9096425-9096485:- ENST00000543258.1_12:9096425-9096485:- M6PR Nonsense Mediated Decay Other GN=M6PR Nonsense Mediated Decay E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep1_Slot1-04_1_3496 40mio-rep2 E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep2_Slot2-5_1_3520.12179.1501.2 2 1 1 13.84 8.64 4.66 5.2 85.3 6 1 1 21800.0 Hydrogen 11 (T) 9 VARSSVVQW (-) 9 KEVSRVQW V/A R|S S/V|V|Q\W 55.6 5 4 5 3 0 dual b/y bias 42.073711 0.812808 516.2825 1031.563 -0.0065 -6.3 -1.3999999999999995 9.72 2108.5 nuORFdb_human ENST00000543258.1_12:9096425-9096485:- ENST00000543258.1_12:9096425-9096485:- M6PR Nonsense Mediated Decay Other GN=M6PR Nonsense Mediated Decay E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep2_Slot2-5_1_3520 40mio-rep3 E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep3_Slot2-5_1_3521.12190.1500.2 2 1 1 10.92 8.75 2.62 2.16 80.9 5 1 1 13700.0 Hydrogen 11 (T) 9 VARSSVVQW (-) 9 TVTQAVVQW V A/R S S/V|V|Q\W 44.4 4 3 4 2 0 unbiased 42.112045 0.813773 516.2821 1031.563 -0.0065 -6.3 -1.7000000000000002 9.72 2108.5 nuORFdb_human ENST00000543258.1_12:9096425-9096485:- ENST00000543258.1_12:9096425-9096485:- M6PR Nonsense Mediated Decay Other GN=M6PR Nonsense Mediated Decay E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep3_Slot2-5_1_3521 40mio-rep4 E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep4_Slot2-5_1_3522.12190.1502.2 2 1 1 10.41 8.74 1.03 1.67 83.2 5 1 1 20800.0 Hydrogen 11 (T) 9 VARSSVVQW (-) 9 KDKVEVQW V A R\S S\V|V|Q|W 44.4 4 5 3 3 0 dual b/y bias 42.097061 0.812094 516.2823 1031.563 -0.0065 -6.3 -1.5999999999999996 9.72 2108.5 nuORFdb_human ENST00000543258.1_12:9096425-9096485:- ENST00000543258.1_12:9096425-9096485:- M6PR Nonsense Mediated Decay Other GN=M6PR Nonsense Mediated Decay E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep4_Slot2-5_1_3522 40mio-rep1 E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep1_Slot1-04_1_3496.7964.1534.2 2 1 1 11.42 9.32 3.76 2.1 79.4 6 1 1 6800.0 Hydrogen 35 (A) 8 VASCGRVF (L) 8 VASNMARF V/A/S|C/G/R V\F 75.0 6 2 5 1 0 y-ion bias Carbamidomethylation C:Carbamidomethylation, 27.516548 0.791835 448.2242 895.445 -0.0047 -5.3 -2.0999999999999996 9.72 6343.3 nuORFdb_human ENST00000520223.1_1_8:41456352-41456517:+ ENST00000520223.1_1_8:41456352-41456517:+ GPAT4 5' uORF 5' uORF GN=GPAT4 5' uORF E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep1_Slot1-04_1_3496 40mio-rep2 E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep2_Slot2-5_1_3520.7651.1552.2 2 1 1 8.48 6.32 3.23 2.16 69.0 5 1 1 8300.0 Hydrogen 35 (A) 8 VASCGRVF (L) 8 VASNMARF V/A/S|C|G R V\F 62.5 5 3 4 2 0 unbiased Carbamidomethylation C:Carbamidomethylation, 26.434767 0.780575 448.2238 895.445 -0.0047 -5.3 -0.39999999999999947 9.72 6343.3 nuORFdb_human ENST00000520223.1_1_8:41456352-41456517:+ ENST00000520223.1_1_8:41456352-41456517:+ GPAT4 5' uORF 5' uORF GN=GPAT4 5' uORF E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep2_Slot2-5_1_3520 40mio-rep3 E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep3_Slot2-5_1_3521.7662.1549.2 2 1 1 9.41 7.73 2.83 1.67 69.2 6 1 1 8860.0 Hydrogen 35 (A) 8 VASCGRVF (L) 8 VASNMARF V/A/S|C/G R\V\F 75.0 6 3 4 1 0 unbiased Carbamidomethylation C:Carbamidomethylation, 26.470228 0.782287 448.2241 895.445 -0.0047 -5.3 -0.7000000000000002 9.72 6343.3 nuORFdb_human ENST00000520223.1_1_8:41456352-41456517:+ ENST00000520223.1_1_8:41456352-41456517:+ GPAT4 5' uORF 5' uORF GN=GPAT4 5' uORF E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep3_Slot2-5_1_3521 40mio-rep4 E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep4_Slot2-5_1_3522.9219.1599.2 2 1 1 8.21 7.1 0.6 1.11 85.5 6 0 1 6600.0 Hydrogen 64 (S) 8 VASISLTK (-) 8 VAISTVKT V/A/S/I/S L\T-K 75.0 6 1 4 0 1 y-ion bias 31.840549 0.751165 409.7495 818.498 -0.0075 -9.1 -4.3999999999999995 8.72 8176.4 nuORFdb_human ENST00000298289.6_1_14:50085755-50087389:- ENST00000298289.6_1_14:50085755-50087389:-|ENST00000639134.1_1_5:18049650-18049725:+ RPL36AL 5' Overlap uORF 5' Overlap uORF GN=RPL36AL 5' Overlap uORF E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep4_Slot2-5_1_3522 20mio-rep1 E_20221201_NO30_400nL_HLAc1_2e7_directIP_titration_rep1_Slot2-4_1_3515.22727.457.1 1 1 1 10.3 7.59 3.74 2.71 84.3 6 0 0 2670.0 Hydrogen 7 (T) 9 VASVIPQSW (A) 9 YQAGPLGPAL V A S/V|I|P\Q\S-W 66.7 6 4 3 2 1 unbiased 78.483185 1.451349 986.5219 986.531 -0.0086 -8.7 -2.499999999999999 5.49 8543.6 smORF_v4|nuORFdb_human ZNF790-AS1_19:37288446-37288670:+ ZNF790-AS1_19:37288446-37288670:+|ENST00000587413.5_1_19:37288433-37288667:+|ENST00000589218.5_1_19:37808810-37809047:+ ZNF790-AS1 lncRNA 19:37288446-37288670:+ 19 ZNF790-AS1 Antisense lncRNA GN=ZNF790-AS1 E_20221201_NO30_400nL_HLAc1_2e7_directIP_titration_rep1_Slot2-4_1_3515 20mio-rep3 E_20221201_NO30_400nL_HLAc1_2e7_directIP_titration_rep4_Slot2-4_1_3519.22781.446.1 1 1 1 11.91 7.68 4.76 4.22 89.8 6 0 0 3320.0 Hydrogen 7 (T) 9 VASVIPQSW (A) 9 LLKDPQSW V A S\V|I|P/Q\S\W 66.7 6 5 3 2 0 unbiased 78.677963 1.457816 986.5264 986.531 -0.0046 -4.6 1.2000000000000002 5.49 8543.6 smORF_v4|nuORFdb_human ZNF790-AS1_19:37288446-37288670:+ ZNF790-AS1_19:37288446-37288670:+|ENST00000587413.5_1_19:37288433-37288667:+|ENST00000589218.5_1_19:37808810-37809047:+ ZNF790-AS1 lncRNA 19:37288446-37288670:+ 19 ZNF790-AS1 Antisense lncRNA GN=ZNF790-AS1 E_20221201_NO30_400nL_HLAc1_2e7_directIP_titration_rep4_Slot2-4_1_3519 40mio-rep1 E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep1_Slot1-04_1_3496.22941.429.1 1 1 1 12.44 7.36 6.02 5.08 87.3 6 0 0 7150.0 Hydrogen 7 (T) 9 VASVIPQSW (A) 9 EKLVCQPL V A/S\V|I|P Q\S\W 55.6 5 5 3 2 0 unbiased 79.251816 1.468135 986.5306 986.531 0.0004 0.4 3.6 5.49 8543.6 smORF_v4|nuORFdb_human ZNF790-AS1_19:37288446-37288670:+ ZNF790-AS1_19:37288446-37288670:+|ENST00000587413.5_1_19:37288433-37288667:+|ENST00000589218.5_1_19:37808810-37809047:+ ZNF790-AS1 lncRNA 19:37288446-37288670:+ 19 ZNF790-AS1 Antisense lncRNA GN=ZNF790-AS1 E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep1_Slot1-04_1_3496 40mio-rep2 E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep2_Slot2-5_1_3520.22691.451.1 1 1 1 10.98 7.45 3.65 3.54 86.0 6 0 0 4530.0 Hydrogen 7 (T) 9 VASVIPQSW (A) 9 LLKDPQSW V A S/V|I|P\Q\S\W 66.7 6 5 3 2 0 unbiased 78.387604 1.455279 986.5262 986.531 -0.0046 -4.6 0.3000000000000007 5.49 8543.6 smORF_v4|nuORFdb_human ZNF790-AS1_19:37288446-37288670:+ ZNF790-AS1_19:37288446-37288670:+|ENST00000587413.5_1_19:37288433-37288667:+|ENST00000589218.5_1_19:37808810-37809047:+ ZNF790-AS1 lncRNA 19:37288446-37288670:+ 19 ZNF790-AS1 Antisense lncRNA GN=ZNF790-AS1 E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep2_Slot2-5_1_3520 40mio-rep3 E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep3_Slot2-5_1_3521.22709.451.1 1 2 1 12.22 7.7 5.66 4.52 84.8 6 0 0 9200.0 Hydrogen 7 (T) 9 VASVIPQSW (A) 9 LLKDPQSW V A/S/V|I|P Q\S\W 55.6 5 4 4 2 0 unbiased 78.446747 1.455321 986.5275 986.531 -0.0026 -2.6 1.9999999999999996 5.49 8543.6 smORF_v4|nuORFdb_human ZNF790-AS1_19:37288446-37288670:+ ZNF790-AS1_19:37288446-37288670:+|ENST00000587413.5_1_19:37288433-37288667:+|ENST00000589218.5_1_19:37808810-37809047:+ ZNF790-AS1 lncRNA 19:37288446-37288670:+ 19 ZNF790-AS1 Antisense lncRNA GN=ZNF790-AS1 E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep3_Slot2-5_1_3521 20mio-rep1 E_20221201_NO30_400nL_HLAc1_2e7_directIP_titration_rep1_Slot2-4_1_3515.12330.1383.2 2 1 1 7.44 4.04 3.27 3.4 66.4 7 1 1 5510.0 Hydrogen 60 (A) 10 VEDPAEARDW (L) 10 EVDPCILNNN V E/D|P\A/E/A/R D-W 60.0 6 2 5 1 1 y-ion bias 42.588341 0.887044 594.2664 1187.533 -0.008 -6.8 -0.5999999999999996 3.91 11433.6 nuORFdb_human ENST00000343150.9_1_9:90341067-90342580:+ ENST00000343150.9_1_9:90341067-90342580:+ CTSL 5' Overlap uORF 5' Overlap uORF GN=CTSL 5' Overlap uORF E_20221201_NO30_400nL_HLAc1_2e7_directIP_titration_rep1_Slot2-4_1_3515 20mio-rep2 E_20221201_NO30_400nL_HLAc1_2e7_directIP_titration_rep3_Slot2-4_1_3518.12450.1380.2 2 1 1 13.94 5.99 7.66 7.95 82.0 8 1 1 5350.0 Hydrogen 60 (A) 10 VEDPAEARDW (L) 10 VEDmQWAHR V/E/D|P/A/E|A/R D\W 80.0 8 3 7 2 0 y-ion bias 43.003677 0.888704 594.2666 1187.533 -0.006 -5.1 1.2000000000000002 3.91 11433.6 nuORFdb_human ENST00000343150.9_1_9:90341067-90342580:+ ENST00000343150.9_1_9:90341067-90342580:+ CTSL 5' Overlap uORF 5' Overlap uORF GN=CTSL 5' Overlap uORF E_20221201_NO30_400nL_HLAc1_2e7_directIP_titration_rep3_Slot2-4_1_3518 20mio-rep3 E_20221201_NO30_400nL_HLAc1_2e7_directIP_titration_rep4_Slot2-4_1_3519.12343.1379.2 2 2 1 14.55 6.8 8.29 7.75 79.3 8 1 1 7930.0 Hydrogen 60 (A) 10 VEDPAEARDW (L) 10 DLnDVYVYW V/E|D|P/A|E|A/R D\W 80.0 8 5 7 4 0 dual b/y bias 42.637436 0.889249 594.2684 1187.533 -0.004 -3.4 2.4 3.91 11433.6 nuORFdb_human ENST00000343150.9_1_9:90341067-90342580:+ ENST00000343150.9_1_9:90341067-90342580:+ CTSL 5' Overlap uORF 5' Overlap uORF GN=CTSL 5' Overlap uORF E_20221201_NO30_400nL_HLAc1_2e7_directIP_titration_rep4_Slot2-4_1_3519 40mio-rep1 E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep1_Slot1-04_1_3496.12635.1346.2 2 2 1 12.69 5.72 6.81 6.97 79.6 7 1 1 16400.0 Hydrogen 60 (A) 10 VEDPAEARDW (L) 10 VEnPSPIRnC V/E|D|P/A|E|A/R D W 70.0 7 4 7 4 0 dual b/y bias 43.645863 0.909845 594.2685 1187.533 -0.004 -3.4 -0.19999999999999973 3.91 11433.6 nuORFdb_human ENST00000343150.9_1_9:90341067-90342580:+ ENST00000343150.9_1_9:90341067-90342580:+ CTSL 5' Overlap uORF 5' Overlap uORF GN=CTSL 5' Overlap uORF E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep1_Slot1-04_1_3496 40mio-rep2 E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep2_Slot2-5_1_3520.12297.1366.2 2 1 1 7.99 4.47 3.29 3.53 63.3 7 1 1 8360.0 Hydrogen 60 (A) 10 VEDPAEARDW (L) 10 VEnPSPIRnC V/E/D|P/A/E/A/R D W 70.0 7 1 7 1 0 y-ion bias 42.48098 0.897648 594.2683 1187.533 -0.004 -3.4 1.5000000000000004 3.91 11433.6 nuORFdb_human ENST00000343150.9_1_9:90341067-90342580:+ ENST00000343150.9_1_9:90341067-90342580:+ CTSL 5' Overlap uORF 5' Overlap uORF GN=CTSL 5' Overlap uORF E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep2_Slot2-5_1_3520 40mio-rep3 E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep3_Slot2-5_1_3521.12319.1374.2 2 1 1 10.32 5.6 4.63 4.71 74.4 7 1 1 8090.0 Hydrogen 60 (A) 10 VEDPAEARDW (L) 10 VEnPSPIRnC V/E/D|P|A/E|A/R D W 70.0 7 3 7 3 0 y-ion bias 42.561108 0.89294 594.2681 1187.533 -0.004 -3.4 1.1999999999999997 3.91 11433.6 nuORFdb_human ENST00000343150.9_1_9:90341067-90342580:+ ENST00000343150.9_1_9:90341067-90342580:+ CTSL 5' Overlap uORF 5' Overlap uORF GN=CTSL 5' Overlap uORF E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep3_Slot2-5_1_3521 40mio-rep4 E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep4_Slot2-5_1_3522.12305.1364.2 2 1 1 10.26 4.73 4.64 5.53 70.6 7 1 1 7720.0 Hydrogen 60 (A) 10 VEDPAEARDW (L) 10 VEDFSmPPHL V E|D|P/A/E|A/R D\W 60.0 6 4 6 3 0 unbiased 42.493992 0.89878 594.2667 1187.533 -0.006 -5.1 -0.39999999999999947 3.91 11433.6 nuORFdb_human ENST00000343150.9_1_9:90341067-90342580:+ ENST00000343150.9_1_9:90341067-90342580:+ CTSL 5' Overlap uORF 5' Overlap uORF GN=CTSL 5' Overlap uORF E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep4_Slot2-5_1_3522 10mio-rep2 E_20221201_NO30_400nL_HLAc1_1e7_directIP_titration_rep2_Slot2-3_1_3513.20880.481.1 1 1 1 10.36 9.23 3.41 1.13 85.2 5 0 0 13600.0 Hydrogen 51 (T) 9 VEIQGVIDP (Q) 9 GQIEVVPEV V E I\Q G|V|I-D-P 44.4 4 3 2 2 2 unbiased 72.122993 1.437071 969.5175 969.525 -0.0071 -7.4 -0.7000000000000002 3.66 11677.9 nuORFdb_human ENST00000372291.3_1_20:44698963-44718582:- ENST00000372291.3_1_20:44698963-44718582:-|ENST00000290231.10_1_20:44698960-44708059:- NCOA5 5' Overlap uORF 5' Overlap uORF GN=NCOA5 5' Overlap uORF E_20221201_NO30_400nL_HLAc1_1e7_directIP_titration_rep2_Slot2-3_1_3513 40mio-rep1 E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep1_Slot1-04_1_3496.20958.452.1 1 1 1 10.06 8.55 3.09 1.5 85.7 5 0 0 41000.0 Hydrogen 51 (T) 9 VEIQGVIDP (Q) 9 GQIEVVPEV V E I\Q G|V|I-D-P 44.4 4 3 2 2 2 unbiased 72.399826 1.454637 969.5228 969.525 -0.0021 -2.2 1.0 3.66 11677.9 nuORFdb_human ENST00000372291.3_1_20:44698963-44718582:- ENST00000372291.3_1_20:44698963-44718582:-|ENST00000290231.10_1_20:44698960-44708059:- NCOA5 5' Overlap uORF 5' Overlap uORF GN=NCOA5 5' Overlap uORF E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep1_Slot1-04_1_3496 SCP E_20221201_NO30_400nL_HLAc1_4e7_PDAC_Slot2-6_1_3524.29758.1253.2 2 1 1 11.35 8.61 3.94 2.73 75.9 6 0 0 14900.0 Hydrogen 2 (L) 11 VFFPLHPGLLL (-) 11 FVFALVLALmI V F|F|P\L/H|P G L L\L 45.5 5 5 4 3 0 unbiased 102.812263 0.968077 626.8763 1252.745 -0.0005 -0.4 2.0 6.71 1365.7 nuORFdb_human ENST00000580500.5_1_17:70427332-70441156:- ENST00000580500.5_1_17:70427332-70441156:-|ENST00000580861.1_1_17:70427332-70599388:- LINC00511 lincRNA lncRNA GN=LINC00511 lincRNA E_20221201_NO30_400nL_HLAc1_4e7_PDAC_Slot2-6_1_3524 Eva-50mio-3fr AL20210827_EV_HLA_PDAC_PANFRO413_2D_50e6_W632_classI_fxn3.37459.37459.2 2 1 1 11.75 1.8 9.31 9.95 89.9 8 0 0 12200000.0 Hydrogen 146 (K) 11 VFIHPAASYYF (-) 11 HPRSPSPAGVYF V F\I H\P-A\A\S\Y|Y|F 63.6 7 7 2 2 1 b-ion bias 0.968 100.0 68.18 68.04 0.0 657.8294 1314.652 -0.0001 -0.1 -0.6 6.71 16847.6 nuORFdb_human ENST00000624931.1_1_11:82868238-82868709:+ ENST00000624931.1_1_11:82868238-82868709:+ PCF11 TEC Other GN=PCF11 TEC AL20210827_EV_HLA_PDAC_PANFRO413_2D_50e6_W632_classI_fxn3 SCP E_20221201_NO30_400nL_HLAc1_4e7_PDAC_Slot2-6_1_3524.27042.1243.2 2 1 1 12.71 6.88 4.79 5.83 82.0 6 0 0 10600.0 Hydrogen 146 (K) 11 VFIHPAASYYF (-) 11 EILnLKCNYF V F I H|P A\A\S\Y|Y\F 45.5 5 6 2 2 0 b-ion bias 93.423103 0.974229 657.8286 1314.652 -0.001 -0.8 1.5999999999999999 6.71 16847.6 nuORFdb_human ENST00000624931.1_1_11:82868238-82868709:+ ENST00000624931.1_1_11:82868238-82868709:+ PCF11 TEC Other GN=PCF11 TEC E_20221201_NO30_400nL_HLAc1_4e7_PDAC_Slot2-6_1_3524 Eva-50mio-3fr AL20210827_EV_HLA_PDAC_PANFRO413_2D_50e6_W632_classI_fxn2.29274.29274.2 2 1 1 12.93 5.28 7.66 7.66 85.2 7 0 0 12000000.0 Hydrogen 6 (Q) 11 VFLEPSHTASF (K) 11 FVIENESPSGR V F|L|E/P S/H T-A/S/F 45.5 5 2 6 2 1 y-ion bias 0.992 81.0 54.08 100.0 0.0 617.8093 1234.61 0.0011 0.9 0.6000000000000001 5.24 4098.7 nuORFdb_human ENST00000548657.5_1_12:52645591-52645690:+ ENST00000548657.5_1_12:52645591-52645690:+ KRT7 ncRNA Processed Transcript lncRNA GN=KRT7 ncRNA Processed Transcript AL20210827_EV_HLA_PDAC_PANFRO413_2D_50e6_W632_classI_fxn2 Exploris AL20221201_MKP_PDAC_01.38897.38897.2 2 2 1 16.43 6.45 8.28 9.99 92.8 9 0 0 161000000.0 Hydrogen 6 (Q) 11 VFLEPSHTASF (K) 11 VFLTDVHmKE V/F|L|E/P S/H-T-A/S/F 81.8 9 2 7 2 2 y-ion bias 0.956 91.4 60.33 79.15 0.0 617.8088 1234.61 0.0001 0.1 -0.7000000000000001 5.24 4098.7 nuORFdb_human ENST00000548657.5_1_12:52645591-52645690:+ ENST00000548657.5_1_12:52645591-52645690:+ KRT7 ncRNA Processed Transcript lncRNA GN=KRT7 ncRNA Processed Transcript AL20221201_MKP_PDAC_01 SCP E_20221201_NO30_400nL_HLAc1_4e7_PDAC_Slot2-6_1_3524.17954.1327.2 2 1 1 17.13 9.61 7.52 7.52 91.7 8 0 0 38200.0 Hydrogen 6 (Q) 11 VFLEPSHTASF (K) 11 FVIENESPSGR V/F|L|E|P S/H T-A|S\F 63.6 7 5 6 4 1 dual b/y bias 62.025768 0.922311 617.8081 1234.61 -0.0015 -1.2 1.2 5.24 4098.7 nuORFdb_human ENST00000548657.5_1_12:52645591-52645690:+ ENST00000548657.5_1_12:52645591-52645690:+ KRT7 ncRNA Processed Transcript lncRNA GN=KRT7 ncRNA Processed Transcript E_20221201_NO30_400nL_HLAc1_4e7_PDAC_Slot2-6_1_3524 Exploris_FAIMS VH20221207_MKP_FAIMS2022E_HLAc1_4e7_PDAC.44788.44788.2 2 1 1 15.19 6.33 7.64 8.86 87.6 7 0 0 12600000.0 Hydrogen 6 (Q) 11 VFLEPSHTASF (K) 11 VFLTDVHmKE V F|L|E/P S/H T-A/S/F 45.5 5 2 6 2 1 y-ion bias 0.88 100.0 80.97 120.0 -50.0 617.8077 1234.61 -0.0021 -1.7 0.9000000000000001 5.24 4098.7 nuORFdb_human ENST00000548657.5_1_12:52645591-52645690:+ ENST00000548657.5_1_12:52645591-52645690:+ KRT7 ncRNA Processed Transcript lncRNA GN=KRT7 ncRNA Processed Transcript VH20221207_MKP_FAIMS2022E_HLAc1_4e7_PDAC Eva-50mio-3fr AL20210827_EV_HLA_PDAC_PANFRO413_2D_50e6_W632_classI_fxn3.40685.40706.2 2 2 1 11.29 6.29 3.87 5.0 88.9 7 1 1 148000000.0 Hydrogen 6 (C) 9 VFLETRLAF (-) 9 NKKEIFAFV V/F|L/E/T R-L-A/F 77.8 7 1 5 1 2 y-ion bias 0.998 82.4 73.87 27.45 0.0 548.3135 1095.62 0.0001 0.1 -0.4 5.97 1668.9 nuORFdb_human ENST00000409807.5_1_2:106013113-106013155:- ENST00000409807.5_1_2:106013113-106013155:-|ENST00000322142.12_1_2:106013113-106015436:- FHL2 5' Overlap uORF 5' Overlap uORF GN=FHL2 5' Overlap uORF AL20210827_EV_HLA_PDAC_PANFRO413_2D_50e6_W632_classI_fxn3 Exploris AL20221201_MKP_PDAC_01.60198.60198.2 2 1 1 8.81 5.28 3.81 3.53 70.4 6 1 1 410000000.0 Hydrogen 6 (C) 9 VFLETRLAF (-) 9 VFLLTLFWG V/F|L/E/T R\L A/F 55.6 5 2 5 1 0 y-ion bias 0.996 53.8 91.05 4.01 0.0 548.3138 1095.62 0.0006 0.5 -0.30000000000000004 5.97 1668.9 nuORFdb_human ENST00000409807.5_1_2:106013113-106013155:- ENST00000409807.5_1_2:106013113-106013155:-|ENST00000322142.12_1_2:106013113-106015436:- FHL2 5' Overlap uORF 5' Overlap uORF GN=FHL2 5' Overlap uORF AL20221201_MKP_PDAC_01 Exploris_FAIMS VH20221207_MKP_FAIMS2022E_HLAc1_4e7_PDAC.61586.61586.2 2 1 1 13.63 8.02 6.23 5.61 93.0 8 1 1 53000000.0 Hydrogen 6 (C) 9 VFLETRLAF (-) 9 NKKEIFAFV V/F|L/E/T/R\L-A/F 100.0 9 2 6 1 1 y-ion bias 0.96 89.2 106.1 3.79 -50.0 548.3129 1095.62 -0.0013 -1.1 1.5 5.97 1668.9 nuORFdb_human ENST00000409807.5_1_2:106013113-106013155:- ENST00000409807.5_1_2:106013113-106013155:-|ENST00000322142.12_1_2:106013113-106015436:- FHL2 5' Overlap uORF 5' Overlap uORF GN=FHL2 5' Overlap uORF VH20221207_MKP_FAIMS2022E_HLAc1_4e7_PDAC Eva-50mio-3fr AL20210827_EV_HLA_PDAC_PANFRO413_2D_50e6_W632_classI_fxn3.27253.27253.2 2 2 1 11.9 6.5 6.4 5.4 85.3 6 1 1 28900000.0 Hydrogen 19 (R) 9 VFLKAGASF (E) 9 VFKLAQAY V/F|L/K-A G A|S/F 66.7 6 2 5 2 1 y-ion bias 0.997 95.8 50.47 100.0 0.0 470.2693 939.53 0.0015 1.6 1.1 8.72 6334.0 smORF_v4|nuORFdb_human PI4KB_1:151298654-151298818:- PI4KB_1:151298654-151298818:-|ENST00000368875.6_2_1:151298654-151299818:- PI4KB overlapping.uORF 1:151298654-151298818:- 1 PI4KB 5' Overlap uORF 5' Overlap uORF GN=PI4KB AL20210827_EV_HLA_PDAC_PANFRO413_2D_50e6_W632_classI_fxn3 Exploris AL20221201_MKP_PDAC_01.36069.36203.2 2 1 1 13.59 5.93 7.81 7.66 89.0 7 1 1 114000000.0 Hydrogen 19 (R) 9 VFLKAGASF (E) 9 VFKLAQAY V/F|L/K-A-G A/S|F 77.8 7 2 5 2 2 y-ion bias 0.998 93.6 56.6 30.92 0.0 470.269 939.53 0.0009 0.9 0.09999999999999998 8.72 6334.0 smORF_v4|nuORFdb_human PI4KB_1:151298654-151298818:- PI4KB_1:151298654-151298818:-|ENST00000368875.6_2_1:151298654-151299818:- PI4KB overlapping.uORF 1:151298654-151298818:- 1 PI4KB 5' Overlap uORF 5' Overlap uORF GN=PI4KB AL20221201_MKP_PDAC_01 SCP E_20221201_NO30_400nL_HLAc1_4e7_PDAC_Slot2-6_1_3524.16293.332.1 1 1 1 12.14 3.28 11.4 8.87 80.5 7 1 1 13900.0 Hydrogen 19 (R) 9 VFLKAGASF (E) 9 VFKLAQAY V F/L|K\A\G\A\S\F 77.8 7 6 2 1 0 b-ion bias 56.28809 1.525556 939.5287 939.53 -0.0008 -0.9 1.5 8.72 6334.0 smORF_v4|nuORFdb_human PI4KB_1:151298654-151298818:- PI4KB_1:151298654-151298818:-|ENST00000368875.6_2_1:151298654-151299818:- PI4KB overlapping.uORF 1:151298654-151298818:- 1 PI4KB 5' Overlap uORF 5' Overlap uORF GN=PI4KB E_20221201_NO30_400nL_HLAc1_4e7_PDAC_Slot2-6_1_3524 Exploris_FAIMS VH20221207_MKP_FAIMS2022E_HLAc1_4e7_PDAC.42143.42143.2 2 1 1 11.98 5.65 6.59 6.33 95.7 7 1 1 54100000.0 Hydrogen 19 (R) 9 VFLKAGASF (E) 9 VFKLAQAY V/F|L/K-A-G A/S/F 77.8 7 1 5 1 2 y-ion bias 0.998 96.4 76.95 6.05 -70.0 470.2674 939.53 -0.0024 -2.5 0.10000000000000009 8.72 6334.0 smORF_v4|nuORFdb_human PI4KB_1:151298654-151298818:- PI4KB_1:151298654-151298818:-|ENST00000368875.6_2_1:151298654-151299818:- PI4KB overlapping.uORF 1:151298654-151298818:- 1 PI4KB 5' Overlap uORF 5' Overlap uORF GN=PI4KB VH20221207_MKP_FAIMS2022E_HLAc1_4e7_PDAC Exploris_FAIMS VH20221207_MKP_FAIMS2022E_HLAc1_4e7_PDAC.38072.38072.3 3 1 1 13.67 6.88 6.07 6.79 83.0 6 1 1 2180000.0 Hydrogen 2 (M) 9 VFLQNHVRF (F) 9 FVLQRNVFH V F|L/Q/N/H/V/R F 55.6 5 1 6 1 0 y-ion bias 0.932 100.0 70.77 118.26 -70.0 387.2162 1159.637 -0.0031 -2.7 -0.10000000000000009 9.73 118999.6 nuORFdb_human ENST00000457196.5_1_7:154735861-154782774:- ENST00000457196.5_1_7:154735861-154782774:- PAXIP1 3' Overlap dORF 3' Overlap dORF GN=PAXIP1 3' Overlap dORF VH20221207_MKP_FAIMS2022E_HLAc1_4e7_PDAC Eva-50mio-3fr AL20210827_EV_HLA_PDAC_PANFRO413_2D_50e6_W632_classI_fxn3.17787.17787.2 2 3 2 16.97 1.12 12.61 15.85 86.8 9 0 0 57000000.0 Hydrogen 101 (S) 10 VFSPHGSPHF (-) 10 APPSQRDGHF V/F|S|P/H/G/S/P/H-F 100.0 10 2 8 2 1 y-ion bias 0.997 100.0 33.8 100.0 0.0 556.2704 1111.532 0.0017 1.5 1.0 6.89 11457.9 nuORFdb_human ENST00000376851.7_1_19:51471875-51472205:- ENST00000376851.7_1_19:51471875-51472205:- KLK6 5' uORF 5' uORF GN=KLK6 5' uORF AL20210827_EV_HLA_PDAC_PANFRO413_2D_50e6_W632_classI_fxn3 Exploris AL20221201_MKP_PDAC_01.22270.22402.2 2 3 1 16.63 5.1 10.06 11.54 85.3 8 0 0 721000000.0 Hydrogen 101 (S) 10 VFSPHGSPHF (-) 10 FVSAKAPAcV V/F|S|P/H/G-S/P/H-F 100.0 10 2 7 2 2 y-ion bias 1.0 100.0 37.93 100.0 0.0 556.2695 1111.532 -0.0002 -0.2 -1.0 6.89 11457.9 nuORFdb_human ENST00000376851.7_1_19:51471875-51472205:- ENST00000376851.7_1_19:51471875-51472205:- KLK6 5' uORF 5' uORF GN=KLK6 5' uORF AL20221201_MKP_PDAC_01 SCP E_20221201_NO30_400nL_HLAc1_4e7_PDAC_Slot2-6_1_3524.10391.1361.2 2 9 1 17.49 11.51 10.54 5.98 90.2 7 0 0 196000.0 Hydrogen 101 (S) 10 VFSPHGSPHF (-) 10 VFSHPAGSYF V/F|S|P/H|G S|P H\F 60.0 6 5 6 4 0 dual b/y bias 35.899578 0.901022 556.2711 1111.532 0.0028 2.5 4.9 6.89 11457.9 nuORFdb_human ENST00000376851.7_1_19:51471875-51472205:- ENST00000376851.7_1_19:51471875-51472205:- KLK6 5' uORF 5' uORF GN=KLK6 5' uORF E_20221201_NO30_400nL_HLAc1_4e7_PDAC_Slot2-6_1_3524 Exploris_FAIMS VH20221207_MKP_FAIMS2022E_HLAc1_4e7_PDAC.27983.28121.2 2 1 1 15.11 2.86 14.9 12.25 87.1 7 0 0 8360000.0 Hydrogen 101 (S) 10 VFSPHGSPHF (-) 10 APPSQRDGHF V F|S/P/H/G-S/P/H-F 80.0 8 1 6 1 2 y-ion bias 0.998 92.6 55.82 57.23 -70.0 556.2683 1111.532 -0.0027 -2.4 0.20000000000000018 6.89 11457.9 nuORFdb_human ENST00000376851.7_1_19:51471875-51472205:- ENST00000376851.7_1_19:51471875-51472205:- KLK6 5' uORF 5' uORF GN=KLK6 5' uORF VH20221207_MKP_FAIMS2022E_HLAc1_4e7_PDAC 20mio-rep1 E_20221201_NO30_400nL_HLAc1_2e7_directIP_titration_rep1_Slot2-4_1_3515.16832.1464.2 2 1 1 7.52 5.66 1.07 1.86 62.2 5 1 1 13500.0 Hydrogen 21 (G) 10 VGADRGLVSL (T) 10 INLnGRVSL V G A/D R\G L\V\S\L 30.0 3 4 1 0 0 b-ion bias 58.130344 0.836473 493.7815 986.563 -0.0062 -6.3 -0.09999999999999964 5.81 4826.3 nuORFdb_human ENST00000481743.1_1_10:8095624-8097282:+ ENST00000481743.1_1_10:8095624-8097282:+ GATA3 ncRNA Processed Transcript lncRNA GN=GATA3 ncRNA Processed Transcript E_20221201_NO30_400nL_HLAc1_2e7_directIP_titration_rep1_Slot2-4_1_3515 40mio-rep2 E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep2_Slot2-5_1_3520.26893.532.1 1 2 1 9.87 4.34 7.09 5.53 79.7 7 0 0 6040.0 Hydrogen 214 (T) 9 VGLPGGLTW (V) 9 GVLPGVGPFG V G/L/P\G\G\L\T\W 77.8 7 5 2 0 0 b-ion bias 92.903297 1.407105 899.4947 899.499 -0.0035 -3.9 1.0000000000000004 5.49 24491.3 nuORFdb_human ENST00000414563.6_1_6:157802226-157802931:+ ENST00000414563.6_1_6:157802226-157802931:+ ZDHHC14 5' uORF 5' uORF GN=ZDHHC14 5' uORF E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep2_Slot2-5_1_3520 40mio-rep3 E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep3_Slot2-5_1_3521.26894.539.1 1 1 1 8.48 3.37 5.2 5.12 77.8 6 0 0 2640.0 Hydrogen 214 (T) 9 VGLPGGLTW (V) 9 GIVTQVPW V G/L/P G\G-L\T\W 55.6 5 3 2 0 1 unbiased 92.899696 1.403011 899.4962 899.499 -0.0025 -2.8 1.7999999999999998 5.49 24491.3 nuORFdb_human ENST00000414563.6_1_6:157802226-157802931:+ ENST00000414563.6_1_6:157802226-157802931:+ ZDHHC14 5' uORF 5' uORF GN=ZDHHC14 5' uORF E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep3_Slot2-5_1_3521 40mio-rep4 E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep4_Slot2-5_1_3522.26907.536.1 1 2 1 8.0 3.53 4.47 4.47 77.7 5 0 0 6660.0 Hydrogen 214 (T) 9 VGLPGGLTW (V) 9 TPAREGIR V G/L/P G\G L\T\W 33.3 3 3 2 0 0 unbiased 92.937279 1.404772 899.4951 899.499 -0.0035 -3.9 0.8000000000000003 5.49 24491.3 nuORFdb_human ENST00000414563.6_1_6:157802226-157802931:+ ENST00000414563.6_1_6:157802226-157802931:+ ZDHHC14 5' uORF 5' uORF GN=ZDHHC14 5' uORF E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep4_Slot2-5_1_3522 40mio-rep1 E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep1_Slot1-04_1_3496.30165.255.1 1 1 1 9.45 5.75 3.85 3.71 79.8 6 0 0 8350.0 Hydrogen 77 (H) 10 VLDPFALISV (L) 10 SLVTKQAnLV V L/D/P F A\L\I\S\V 50.0 5 4 2 0 0 b-ion bias 104.209625 1.570842 1073.6203 1073.624 -0.0041 -3.8 -0.5999999999999996 3.8 16267.6 nuORFdb_human ENST00000381962.3_1_20:628669-629104:- ENST00000381962.3_1_20:628669-629104:- SRXN1 3' dORF 3' dORF GN=SRXN1 3' dORF E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep1_Slot1-04_1_3496 20mio-rep1 E_20221201_NO30_400nL_HLAc1_2e7_directIP_titration_rep1_Slot2-4_1_3515.28007.307.1 1 2 1 12.6 7.8 4.0 4.8 88.7 6 0 0 5630.0 Hydrogen 87 (L) 9 VLDVELLAY (C) 9 LVDIDLISF V L D\V\E\L\L\A\Y 66.7 6 6 0 0 0 b-ion bias 96.711136 1.540348 1034.5732 1034.577 -0.0068 -6.6 -0.39999999999999947 3.67 12486.0 nuORFdb_human ENST00000435805.6_1_10:43047060-43047381:- ENST00000435805.6_1_10:43047060-43047381:- ZNF37BP Pseudogene Pseudogene GN=ZNF37BP Pseudogene E_20221201_NO30_400nL_HLAc1_2e7_directIP_titration_rep1_Slot2-4_1_3515 20mio-rep2 E_20221201_NO30_400nL_HLAc1_2e7_directIP_titration_rep3_Slot2-4_1_3518.28016.297.1 1 1 1 11.89 8.97 3.06 2.92 81.6 6 0 0 2390.0 Hydrogen 87 (L) 9 VLDVELLAY (C) 9 LVDIDLISF V L D\V\E|L\L\A\Y 66.7 6 6 1 1 0 b-ion bias 96.765175 1.546229 1034.5695 1034.577 -0.0068 -6.6 -0.2999999999999998 3.67 12486.0 nuORFdb_human ENST00000435805.6_1_10:43047060-43047381:- ENST00000435805.6_1_10:43047060-43047381:- ZNF37BP Pseudogene Pseudogene GN=ZNF37BP Pseudogene E_20221201_NO30_400nL_HLAc1_2e7_directIP_titration_rep3_Slot2-4_1_3518 20mio-rep3 E_20221201_NO30_400nL_HLAc1_2e7_directIP_titration_rep4_Slot2-4_1_3519.28029.304.1 1 2 1 9.4 6.22 2.84 3.18 74.4 6 0 0 4900.0 Hydrogen 87 (L) 9 VLDVELLAY (C) 9 LVDIDLISF V L D\V\E|L\L\A\Y 66.7 6 6 1 1 0 b-ion bias 96.80629 1.541934 1034.5676 1034.577 -0.0068 -6.6 -0.7999999999999998 3.67 12486.0 nuORFdb_human ENST00000435805.6_1_10:43047060-43047381:- ENST00000435805.6_1_10:43047060-43047381:- ZNF37BP Pseudogene Pseudogene GN=ZNF37BP Pseudogene E_20221201_NO30_400nL_HLAc1_2e7_directIP_titration_rep4_Slot2-4_1_3519 40mio-rep1 E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep1_Slot1-04_1_3496.28108.280.1 1 2 1 11.83 7.5 2.67 4.33 85.7 6 0 0 18600.0 Hydrogen 87 (L) 9 VLDVELLAY (C) 9 LVDIDLISF V L D\V\E\L\L\A\Y 66.7 6 6 0 0 0 b-ion bias 97.1054 1.556197 1034.5737 1034.577 -0.0068 -6.6 -3.3999999999999995 3.67 12486.0 nuORFdb_human ENST00000435805.6_1_10:43047060-43047381:- ENST00000435805.6_1_10:43047060-43047381:- ZNF37BP Pseudogene Pseudogene GN=ZNF37BP Pseudogene E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep1_Slot1-04_1_3496 40mio-rep2 E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep2_Slot2-5_1_3520.28010.293.1 1 1 1 11.31 6.81 3.29 4.5 81.7 6 0 0 12000.0 Hydrogen 87 (L) 9 VLDVELLAY (C) 9 LVDIDLISF V L D\V\E\L\L\A\Y 66.7 6 6 0 0 0 b-ion bias 96.7631 1.54851 1034.5727 1034.577 -0.0068 -6.6 -1.6999999999999993 3.67 12486.0 nuORFdb_human ENST00000435805.6_1_10:43047060-43047381:- ENST00000435805.6_1_10:43047060-43047381:- ZNF37BP Pseudogene Pseudogene GN=ZNF37BP Pseudogene E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep2_Slot2-5_1_3520 40mio-rep3 E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep3_Slot2-5_1_3521.28011.297.1 1 1 1 11.13 5.96 4.14 5.17 83.9 6 0 0 5860.0 Hydrogen 87 (L) 9 VLDVELLAY (C) 9 LVDIDLISF V L D\V\E\L\L\A\Y 66.7 6 6 0 0 0 b-ion bias 96.759155 1.54619 1034.5709 1034.577 -0.0068 -6.6 -2.0 3.67 12486.0 nuORFdb_human ENST00000435805.6_1_10:43047060-43047381:- ENST00000435805.6_1_10:43047060-43047381:- ZNF37BP Pseudogene Pseudogene GN=ZNF37BP Pseudogene E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep3_Slot2-5_1_3521 40mio-rep1 E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep1_Slot1-04_1_3496.15219.1395.2 2 1 1 7.72 5.16 3.06 2.57 56.4 7 1 1 9790.0 Hydrogen 19 (R) 9 VLLERLENT (R) 9 VLISLPESGAT V/L/L/E/R L\E\N\T 77.8 7 3 4 0 0 unbiased 52.570496 0.879389 543.81 1086.615 -0.0026 -2.4 0.8000000000000003 4.53 8053.2 nuORFdb_human ENST00000524003.1_1_8:92081749-92081950:- ENST00000524003.1_1_8:92081749-92081950:-|ENST00000522817.1_1_8:92075962-92081953:- OTUD6B-AS1 Antisense lncRNA GN=OTUD6B-AS1 Antisense E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep1_Slot1-04_1_3496 Exploris_FAIMS VH20221207_MKP_FAIMS2022E_HLAc1_4e7_PDAC.64742.64742.2 2 1 1 9.12 5.61 2.45 3.51 75.9 6 0 0 2810000.0 Hydrogen 16 (N) 10 VLPAGLVLEF (E) 10 VLPQLLLDF V L P-A G\L\V\L\E/F 50.0 5 4 1 0 1 b-ion bias 0.992 100.0 110.5 99.18 -70.0 529.3171 1057.629 -0.0023 -2.2 0.3999999999999999 4.0 7549.2 nuORFdb_human ENST00000435682.1_1_2:21912159-21933190:- ENST00000435682.1_1_2:21912159-21933190:- LINC01822 lincRNA lncRNA GN=LINC01822 lincRNA VH20221207_MKP_FAIMS2022E_HLAc1_4e7_PDAC 20mio-rep3 E_20221201_NO30_400nL_HLAc1_2e7_directIP_titration_rep4_Slot2-4_1_3519.17433.1336.2 2 1 1 8.32 5.71 3.31 2.61 72.8 7 0 0 3250.0 Hydrogen 33 (A) 11 VLPDQHLVTTV (-) 11 LVLSSPHPATIS V/L/P D Q-H\L-V|T\T V 54.5 6 3 3 1 2 unbiased 60.212421 0.916179 611.3419 1221.684 -0.007 -5.8 0.0 5.08 4530.1 smORF_v4|nuORFdb_human LOC105369340_11:64013412-64013543:+ LOC105369340_11:64013412-64013543:+|ENST00000544553.1_1_11:64013453-64013543:+ LOC105369340 lncRNA 11:64013412-64013543:+ 11 RP11-783K16.5 Antisense lncRNA GN=LOC105369340 E_20221201_NO30_400nL_HLAc1_2e7_directIP_titration_rep4_Slot2-4_1_3519 SCP E_20221201_NO30_400nL_HLAc1_4e7_PDAC_Slot2-6_1_3524.30649.252.1 1 1 1 12.68 8.15 5.63 4.53 90.5 8 0 0 40200.0 Hydrogen 5 (R) 9 VLPFPLILF (F) 9 VFPIPILFI V/L/P\F/P\L\I\L\F 100.0 9 5 3 0 0 unbiased 105.889847 1.57249 1058.6638 1058.665 -0.0049 -4.6 -2.1999999999999997 5.49 2497.0 nuORFdb_human ENST00000619858.1_1_17:36886919-36886982:+ ENST00000619858.1_1_17:36886919-36886982:+ CISD3 ncRNA Processed Transcript lncRNA GN=CISD3 ncRNA Processed Transcript E_20221201_NO30_400nL_HLAc1_4e7_PDAC_Slot2-6_1_3524 SCP E_20221201_NO30_400nL_HLAc1_4e7_PDAC_Slot2-6_1_3524.10498.1323.2 2 1 1 13.07 7.43 6.01 5.64 95.3 8 1 2 11500.0 Hydrogen 28 (T) 9 VLPKKAYLM (-) 9 LVFDLLVIM V/L/P/K/K-A\Y\L\M 100.0 9 3 4 0 1 unbiased 36.268982 0.924322 531.822 1062.638 -0.0013 -1.2 1.2 9.7 4109.9 nuORFdb_human ENST00000381116.5_1_13:28015203-28024035:- ENST00000381116.5_1_13:28015203-28024035:-|ENST00000485650.5_1_13:28015203-28024670:- MTIF3 5' uORF 5' uORF GN=MTIF3 5' uORF E_20221201_NO30_400nL_HLAc1_4e7_PDAC_Slot2-6_1_3524 SCP E_20221201_NO30_400nL_HLAc1_4e7_PDAC_Slot2-6_1_3524.7424.1344.2 2 2 1 10.3 7.99 1.78 2.32 82.2 6 1 2 10500.0 M36m Hydrogen 28 (T) 9 VLPKKAYLm (-) 9 VLRRmKmI V/L/P/K K A/Y/L\m 66.7 6 1 5 0 0 y-ion bias m:Oxidized methionine 25.651018 0.911569 539.8172 1062.638 15.9887 -5.7 -3.3000000000000003 9.7 4109.9 nuORFdb_human ENST00000381116.5_1_13:28015203-28024035:- ENST00000381116.5_1_13:28015203-28024035:-|ENST00000485650.5_1_13:28015203-28024670:- MTIF3 5' uORF 5' uORF GN=MTIF3 5' uORF E_20221201_NO30_400nL_HLAc1_4e7_PDAC_Slot2-6_1_3524 Eva-50mio-3fr AL20210827_EV_HLA_PDAC_PANFRO413_2D_50e6_W632_classI_fxn3.28722.28722.2 2 2 1 12.31 8.1 4.2 4.2 86.8 8 1 2 40200000.0 Hydrogen 27 (T) 9 VLPKKGWLL (F) 9 GLASRKPLVL V/L|P/K/K-G-W/L/L 100.0 9 1 6 1 2 y-ion bias 0.979 95.5 53.03 100.0 0.0 527.3447 1053.682 0.0001 0.1 -0.4 10.0 4217.1 smORF_v4|nuORFdb_human MTIF3_13:28024646-28014586:- MTIF3_13:28024646-28014586:-|ENST00000493719.5_1_13:28014569-28024213:-|ENST00000461838.5_1_13:28014569-28024229:-|ENST00000464753.1_1_13:28014569-28024306:-|ENST00000381120.7_1_13:28014569-28024670:-|ENST00000484342.2_1_13:28014569-28024692:- MTIF3 overlapping.uORF 13:28024646-28014586:- 13 MTIF3 5' Overlap uORF 5' Overlap uORF GN=MTIF3 AL20210827_EV_HLA_PDAC_PANFRO413_2D_50e6_W632_classI_fxn3 Exploris AL20221201_MKP_PDAC_01.55945.55945.2 2 5 1 13.11 9.16 5.91 3.95 79.1 7 1 2 556000000.0 Hydrogen 27 (T) 9 VLPKKGWLL (F) 9 VLKPLWKGI V/L|P/K/K-G W/L/L 77.8 7 1 6 1 1 y-ion bias 0.915 73.5 84.75 100.0 0.0 527.3449 1053.682 0.0006 0.5 -0.30000000000000004 10.0 4217.1 smORF_v4|nuORFdb_human MTIF3_13:28024646-28014586:- MTIF3_13:28024646-28014586:-|ENST00000493719.5_1_13:28014569-28024213:-|ENST00000461838.5_1_13:28014569-28024229:-|ENST00000464753.1_1_13:28014569-28024306:-|ENST00000381120.7_1_13:28014569-28024670:-|ENST00000484342.2_1_13:28014569-28024692:- MTIF3 overlapping.uORF 13:28024646-28014586:- 13 MTIF3 5' Overlap uORF 5' Overlap uORF GN=MTIF3 AL20221201_MKP_PDAC_01 SCP E_20221201_NO30_400nL_HLAc1_4e7_PDAC_Slot2-6_1_3524.26668.1347.2 2 2 1 12.41 9.69 2.79 2.71 92.8 8 1 2 57100.0 Hydrogen 27 (T) 9 VLPKKGWLL (F) 9 LPFRVIPLV V/L/P/K/K-G\W\L-L 100.0 9 2 4 0 2 y-ion bias 92.131729 0.909429 527.3438 1053.682 -0.0012 -1.1 1.2999999999999998 10.0 4217.1 smORF_v4|nuORFdb_human MTIF3_13:28024646-28014586:- MTIF3_13:28024646-28014586:-|ENST00000493719.5_1_13:28014569-28024213:-|ENST00000461838.5_1_13:28014569-28024229:-|ENST00000464753.1_1_13:28014569-28024306:-|ENST00000381120.7_1_13:28014569-28024670:-|ENST00000484342.2_1_13:28014569-28024692:- MTIF3 overlapping.uORF 13:28024646-28014586:- 13 MTIF3 5' Overlap uORF 5' Overlap uORF GN=MTIF3 E_20221201_NO30_400nL_HLAc1_4e7_PDAC_Slot2-6_1_3524 SCP E_20221201_NO30_400nL_HLAc1_4e7_PDAC_Slot2-6_1_3524.29516.1166.2 2 1 1 13.96 9.16 3.66 4.79 91.6 7 1 2 10300.0 Hydrogen 27 (T) 11 VLPKKGWLLFF (-) 11 VILVVVIIGEFF V/L/P K/K G W|L\L|F\F 54.5 6 4 5 2 0 unbiased 101.976654 1.022131 674.4121 1347.819 -0.002 -1.5 0.8999999999999999 10.0 4217.1 smORF_v4|nuORFdb_human MTIF3_13:28024646-28014586:- MTIF3_13:28024646-28014586:-|ENST00000493719.5_1_13:28014569-28024213:-|ENST00000461838.5_1_13:28014569-28024229:-|ENST00000464753.1_1_13:28014569-28024306:-|ENST00000381120.7_1_13:28014569-28024670:-|ENST00000484342.2_1_13:28014569-28024692:- MTIF3 overlapping.uORF 13:28024646-28014586:- 13 MTIF3 5' Overlap uORF 5' Overlap uORF GN=MTIF3 E_20221201_NO30_400nL_HLAc1_4e7_PDAC_Slot2-6_1_3524 40mio-rep3 E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep3_Slot2-5_1_3521.25878.690.1 1 2 1 7.25 3.81 0.66 3.44 56.2 6 0 0 25000.0 Hydrogen 42 (T) 8 VLPVSPAE (K) 8 VVPLnPAT V/L/P|V|S\P A\E 75.0 6 4 4 2 0 unbiased 89.391563 1.312056 811.4546 811.456 -0.001 -1.2 3.3999999999999995 4.6 8032.0 nuORFdb_human ENST00000566848.5_1_16:29974022-29974241:+ ENST00000566848.5_1_16:29974022-29974241:+ TMEM219 5' uORF 5' uORF GN=TMEM219 5' uORF E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep3_Slot2-5_1_3521 SCP E_20221201_NO30_400nL_HLAc1_4e7_PDAC_Slot2-6_1_3524.22317.544.1 1 1 1 9.68 6.39 4.44 3.29 64.2 8 0 0 5580.0 Hydrogen 7 (P) 9 VLSGPVPSF (S) 9 VLDPKPSF V/L/S/G/P\V|P\S\F 100.0 9 4 5 1 0 unbiased 77.100616 1.399983 902.4969 902.498 -0.0012 -1.3 1.0999999999999999 5.49 3166.5 nuORFdb_human ENST00000540589.2_1_12:113357371-113369720:+ ENST00000540589.2_1_12:113357371-113369720:+|ENST00000202917.9_1_12:113357194-113357533:+ OAS1 3' Overlap dORF 3' Overlap dORF GN=OAS1 3' Overlap dORF E_20221201_NO30_400nL_HLAc1_4e7_PDAC_Slot2-6_1_3524 SCP E_20221201_NO30_400nL_HLAc1_4e7_PDAC_Slot2-6_1_3524.27738.1475.2 2 1 1 7.08 5.26 0.31 1.82 54.8 5 0 0 14900.0 Hydrogen 74 (S) 9 VLSHPPPSL (S) 9 VLMLnRSL V/L/S/H P/P P S\L 44.4 4 1 4 0 0 y-ion bias 95.830162 0.829511 473.7701 946.536 -0.0029 -3.1 -0.7000000000000002 6.71 70256.7 nuORFdb_human ENST00000477970.1_1_1:44078891-44086093:+ ENST00000477970.1_1_1:44078891-44086093:+ PTPRF ncRNA Processed Transcript lncRNA GN=PTPRF ncRNA Processed Transcript E_20221201_NO30_400nL_HLAc1_4e7_PDAC_Slot2-6_1_3524 SCP E_20221201_NO30_400nL_HLAc1_4e7_PDAC_Slot2-6_1_3524.17381.456.1 1 2 1 11.35 4.74 6.22 6.6 75.4 7 0 0 17200.0 Hydrogen 43 (C) 9 VLSTPSTTF (C) 9 LLSSPSYSV V L/S/T/P\S|T\T\F 77.8 7 4 4 1 0 unbiased 60.04649 1.452125 952.4978 952.499 -0.0006 -0.6 1.7999999999999998 5.49 8249.2 nuORFdb_human ENST00000601423.5_2_19:51010936-51014415:- ENST00000601423.5_2_19:51010936-51014415:-|ENST00000598418.5_2_19:51010936-51014415:-|ENST00000594350.1_1_19:51010936-51014415:-|ENST00000595669.5_2_19:51009791-51014415:- JOSD2 5' Overlap uORF 5' Overlap uORF GN=JOSD2 5' Overlap uORF E_20221201_NO30_400nL_HLAc1_4e7_PDAC_Slot2-6_1_3524 10mio-rep1 E_20221201_NO30_400nL_HLAc1_1e7_directIP_titration_rep1_Slot2-3_1_3512.18245.1462.2 2 1 1 11.35 8.23 4.05 3.12 81.0 6 1 1 5700.0 Hydrogen 4 (Q) 9 VLWRGDSPL (V) 9 VLFGFYSLP V/L/W/R|G D|S\P-L 77.8 7 3 5 2 1 unbiased 63.025711 0.837332 521.7824 1042.568 -0.0113 -10.8 -3.1000000000000005 5.81 2721.2 nuORFdb_human TCONS_00008378_4:189081449-189081692:+ TCONS_00008378_4:189081449-189081692:+ linc-FRG1-12 lincRNA lncRNA GN=linc-FRG1-12 lincRNA E_20221201_NO30_400nL_HLAc1_1e7_directIP_titration_rep1_Slot2-3_1_3512 10mio-rep2 E_20221201_NO30_400nL_HLAc1_1e7_directIP_titration_rep2_Slot2-3_1_3513.18187.1467.2 2 2 1 12.35 7.8 5.37 4.55 84.9 7 1 1 15100.0 Hydrogen 4 (Q) 9 VLWRGDSPL (V) 9 VLGLADMGGPL V/L/W/R\G D|S|P\L 77.8 7 4 5 2 0 unbiased 62.822132 0.83453 521.7831 1042.568 -0.0093 -8.9 -2.2 5.81 2721.2 nuORFdb_human TCONS_00008378_4:189081449-189081692:+ TCONS_00008378_4:189081449-189081692:+ linc-FRG1-12 lincRNA lncRNA GN=linc-FRG1-12 lincRNA E_20221201_NO30_400nL_HLAc1_1e7_directIP_titration_rep2_Slot2-3_1_3513 10mio-rep3 E_20221201_NO30_400nL_HLAc1_1e7_directIP_titration_rep3_Slot2-3_1_3514.18132.1469.2 2 1 1 15.01 8.8 6.69 6.21 88.0 7 1 1 5870.0 Hydrogen 4 (Q) 9 VLWRGDSPL (V) 9 VLFGFYSLP V/L/W|R|G D|S|P\L 77.8 7 5 6 4 0 dual b/y bias 62.634815 0.832961 521.7849 1042.568 -0.0053 -5.1 1.2000000000000002 5.81 2721.2 nuORFdb_human TCONS_00008378_4:189081449-189081692:+ TCONS_00008378_4:189081449-189081692:+ linc-FRG1-12 lincRNA lncRNA GN=linc-FRG1-12 lincRNA E_20221201_NO30_400nL_HLAc1_1e7_directIP_titration_rep3_Slot2-3_1_3514 20mio-rep1 E_20221201_NO30_400nL_HLAc1_2e7_directIP_titration_rep1_Slot2-4_1_3515.18153.1457.2 2 2 1 13.42 8.49 5.95 4.93 84.1 7 1 1 20100.0 Hydrogen 4 (Q) 9 VLWRGDSPL (V) 9 VLGLADMGGPL V/L/W/R|G D|S|P\L 77.8 7 4 6 3 0 dual b/y bias 62.692986 0.840812 521.7848 1042.568 -0.0053 -5.1 1.1000000000000005 5.81 2721.2 nuORFdb_human TCONS_00008378_4:189081449-189081692:+ TCONS_00008378_4:189081449-189081692:+ linc-FRG1-12 lincRNA lncRNA GN=linc-FRG1-12 lincRNA E_20221201_NO30_400nL_HLAc1_2e7_directIP_titration_rep1_Slot2-4_1_3515 20mio-rep2 E_20221201_NO30_400nL_HLAc1_2e7_directIP_titration_rep3_Slot2-4_1_3518.18215.1460.2 2 3 1 13.41 10.22 3.67 3.19 85.5 7 1 1 31600.0 Hydrogen 4 (Q) 9 VLWRGDSPL (V) 9 VLGLADMGGPL V/L/W/R/G D|S|P\L 77.8 7 3 6 2 0 y-ion bias 62.916481 0.838652 521.7835 1042.568 -0.0093 -8.9 -2.6000000000000005 5.81 2721.2 nuORFdb_human TCONS_00008378_4:189081449-189081692:+ TCONS_00008378_4:189081449-189081692:+ linc-FRG1-12 lincRNA lncRNA GN=linc-FRG1-12 lincRNA E_20221201_NO30_400nL_HLAc1_2e7_directIP_titration_rep3_Slot2-4_1_3518 20mio-rep3 E_20221201_NO30_400nL_HLAc1_2e7_directIP_titration_rep4_Slot2-4_1_3519.18200.1455.2 2 2 1 10.74 7.33 4.42 3.41 80.8 7 1 1 25100.0 Hydrogen 4 (Q) 9 VLWRGDSPL (V) 9 VLRCVGEPL V/L/W/R\G D|S|P\L 77.8 7 4 5 2 0 unbiased 62.860088 0.841703 521.7841 1042.568 -0.0073 -7.0 -1.2000000000000002 5.81 2721.2 nuORFdb_human TCONS_00008378_4:189081449-189081692:+ TCONS_00008378_4:189081449-189081692:+ linc-FRG1-12 lincRNA lncRNA GN=linc-FRG1-12 lincRNA E_20221201_NO30_400nL_HLAc1_2e7_directIP_titration_rep4_Slot2-4_1_3519 40mio-rep1 E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep1_Slot1-04_1_3496.18427.1433.2 2 3 1 15.88 10.89 5.55 4.99 86.1 7 1 1 50800.0 Hydrogen 4 (Q) 9 VLWRGDSPL (V) 9 VLGLADMGGPL V/L/W|R\G D|S|P\L 77.8 7 5 5 3 0 dual b/y bias 63.653294 0.855943 521.7867 1042.568 -0.0013 -1.2 2.0 5.81 2721.2 nuORFdb_human TCONS_00008378_4:189081449-189081692:+ TCONS_00008378_4:189081449-189081692:+ linc-FRG1-12 lincRNA lncRNA GN=linc-FRG1-12 lincRNA E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep1_Slot1-04_1_3496 40mio-rep2 E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep2_Slot2-5_1_3520.18068.1455.2 2 4 1 12.43 7.55 5.83 4.88 76.5 7 1 1 54000.0 Hydrogen 4 (Q) 9 VLWRGDSPL (V) 9 VLGLADMGGPL V/L/W/R\G D|S|P\L 77.8 7 4 5 2 0 unbiased 62.414707 0.841562 521.7848 1042.568 -0.0053 -5.1 -0.1999999999999993 5.81 2721.2 nuORFdb_human TCONS_00008378_4:189081449-189081692:+ TCONS_00008378_4:189081449-189081692:+ linc-FRG1-12 lincRNA lncRNA GN=linc-FRG1-12 lincRNA E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep2_Slot2-5_1_3520 40mio-rep3 E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep3_Slot2-5_1_3521.18101.1452.2 2 3 1 16.0 10.45 5.54 5.54 88.3 7 1 1 42700.0 Hydrogen 4 (Q) 9 VLWRGDSPL (V) 9 VLGLADMGGPL V/L/W/R|G D|S|P\L 77.8 7 4 6 3 0 dual b/y bias 62.529636 0.843916 521.785 1042.568 -0.0053 -5.1 -0.5 5.81 2721.2 nuORFdb_human TCONS_00008378_4:189081449-189081692:+ TCONS_00008378_4:189081449-189081692:+ linc-FRG1-12 lincRNA lncRNA GN=linc-FRG1-12 lincRNA E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep3_Slot2-5_1_3521 40mio-rep4 E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep4_Slot2-5_1_3522.18091.1453.2 2 3 1 14.26 8.67 6.19 5.59 83.5 7 1 1 37300.0 Hydrogen 4 (Q) 9 VLWRGDSPL (V) 9 VLGLADMGGPL V/L/W/R|G D|S|P\L 77.8 7 4 6 3 0 dual b/y bias 62.477459 0.843035 521.7854 1042.568 -0.0053 -5.1 -0.39999999999999947 5.81 2721.2 nuORFdb_human TCONS_00008378_4:189081449-189081692:+ TCONS_00008378_4:189081449-189081692:+ linc-FRG1-12 lincRNA lncRNA GN=linc-FRG1-12 lincRNA E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep4_Slot2-5_1_3522 5mio-rep1 E_20221201_NO30_400nL_HLAc1_5e6_directIP_titration_rep1_Slot1-37_1_3502.18500.1487.2 2 2 1 9.69 6.78 2.91 2.91 75.7 6 1 1 10800.0 Hydrogen 4 (Q) 9 VLWRGDSPL (V) 9 VISTCPRPL V/L/W/R G D\S|P\L 66.7 6 3 4 1 0 unbiased 63.899525 0.821732 521.7845 1042.568 -0.0073 -7.0 0.0 5.81 2721.2 nuORFdb_human TCONS_00008378_4:189081449-189081692:+ TCONS_00008378_4:189081449-189081692:+ linc-FRG1-12 lincRNA lncRNA GN=linc-FRG1-12 lincRNA E_20221201_NO30_400nL_HLAc1_5e6_directIP_titration_rep1_Slot1-37_1_3502 5mio-rep2 E_20221201_NO30_400nL_HLAc1_5e6_directIP_titration_rep2_Slot2-2_1_3510.18349.1477.2 2 1 1 9.07 5.81 4.3 3.27 65.4 7 1 1 2440.0 Hydrogen 4 (Q) 9 VLWRGDSPL (V) 9 VLFGFYSLP V/L/W/R\G D|S|P\L 77.8 7 4 5 2 0 unbiased 63.377232 0.828297 521.7833 1042.568 -0.0093 -8.9 -0.20000000000000107 5.81 2721.2 nuORFdb_human TCONS_00008378_4:189081449-189081692:+ TCONS_00008378_4:189081449-189081692:+ linc-FRG1-12 lincRNA lncRNA GN=linc-FRG1-12 lincRNA E_20221201_NO30_400nL_HLAc1_5e6_directIP_titration_rep2_Slot2-2_1_3510 10mio-rep1 E_20221201_NO30_400nL_HLAc1_1e7_directIP_titration_rep1_Slot2-3_1_3512.17486.1436.2 2 1 1 14.23 8.44 6.73 5.79 83.5 8 1 1 4320.0 Hydrogen 8 (V) 9 VLYLRQVGY (L) 9 VLQLTFGIGY V/L/Y|L|R\Q\V|G\Y 100.0 9 6 5 3 0 dual b/y bias 60.402988 0.853513 555.8135 1110.631 -0.0099 -8.9 -1.2000000000000002 8.56 2583.1 nuORFdb_human ENST00000311895.7_1_16:14015972-14016038:+ ENST00000311895.7_1_16:14015972-14016038:+ ERCC4 Out-of-Frame Out-of-Frame GN=ERCC4 Out-of-Frame E_20221201_NO30_400nL_HLAc1_1e7_directIP_titration_rep1_Slot2-3_1_3512 10mio-rep2 E_20221201_NO30_400nL_HLAc1_1e7_directIP_titration_rep2_Slot2-3_1_3513.17429.1438.2 2 1 1 17.54 8.62 10.77 8.92 87.9 8 1 1 5230.0 Hydrogen 8 (V) 9 VLYLRQVGY (L) 9 VLQLTFGIGY V/L/Y|L|R\Q|V|G\Y 100.0 9 6 6 4 0 dual b/y bias 60.205093 0.852249 555.8171 1110.631 -0.0039 -3.5 3.2 8.56 2583.1 nuORFdb_human ENST00000311895.7_1_16:14015972-14016038:+ ENST00000311895.7_1_16:14015972-14016038:+ ERCC4 Out-of-Frame Out-of-Frame GN=ERCC4 Out-of-Frame E_20221201_NO30_400nL_HLAc1_1e7_directIP_titration_rep2_Slot2-3_1_3513 10mio-rep3 E_20221201_NO30_400nL_HLAc1_1e7_directIP_titration_rep3_Slot2-3_1_3514.17392.1446.2 2 1 1 12.25 7.64 2.17 4.61 75.9 6 1 1 5430.0 Hydrogen 8 (V) 9 VLYLRQVGY (L) 9 VLQLTFGIGY V/L/Y|L|R Q V\G\Y 66.7 6 4 4 2 0 unbiased 60.077003 0.847725 555.8144 1110.631 -0.0099 -8.9 -2.6000000000000005 8.56 2583.1 nuORFdb_human ENST00000311895.7_1_16:14015972-14016038:+ ENST00000311895.7_1_16:14015972-14016038:+ ERCC4 Out-of-Frame Out-of-Frame GN=ERCC4 Out-of-Frame E_20221201_NO30_400nL_HLAc1_1e7_directIP_titration_rep3_Slot2-3_1_3514 20mio-rep1 E_20221201_NO30_400nL_HLAc1_2e7_directIP_titration_rep1_Slot2-4_1_3515.17411.1432.2 2 2 1 16.57 9.11 8.34 7.46 89.1 8 1 1 11400.0 Hydrogen 8 (V) 9 VLYLRQVGY (L) 9 VLQLTFGIGY V/L/Y|L/R\Q|V|G\Y 100.0 9 5 6 3 0 dual b/y bias 60.13147 0.856469 555.8162 1110.631 -0.0059 -5.3 0.9000000000000004 8.56 2583.1 nuORFdb_human ENST00000311895.7_1_16:14015972-14016038:+ ENST00000311895.7_1_16:14015972-14016038:+ ERCC4 Out-of-Frame Out-of-Frame GN=ERCC4 Out-of-Frame E_20221201_NO30_400nL_HLAc1_2e7_directIP_titration_rep1_Slot2-4_1_3515 20mio-rep2 E_20221201_NO30_400nL_HLAc1_2e7_directIP_titration_rep3_Slot2-4_1_3518.17478.1435.2 2 1 1 14.56 8.2 6.41 6.36 87.9 8 1 1 4620.0 Hydrogen 8 (V) 9 VLYLRQVGY (L) 9 VLQLTFGIGY V/L/Y|L/R\Q\V\G\Y 100.0 9 5 4 1 0 unbiased 60.371269 0.854155 555.8164 1110.631 -0.0059 -5.3 1.0 8.56 2583.1 nuORFdb_human ENST00000311895.7_1_16:14015972-14016038:+ ENST00000311895.7_1_16:14015972-14016038:+ ERCC4 Out-of-Frame Out-of-Frame GN=ERCC4 Out-of-Frame E_20221201_NO30_400nL_HLAc1_2e7_directIP_titration_rep3_Slot2-4_1_3518 20mio-rep3 E_20221201_NO30_400nL_HLAc1_2e7_directIP_titration_rep4_Slot2-4_1_3519.17458.1430.2 2 1 1 15.09 7.11 9.44 7.98 81.5 8 1 1 6110.0 Hydrogen 8 (V) 9 VLYLRQVGY (L) 9 VLQLTFGIGY V/L/Y/L|R\Q|V\G\Y 100.0 9 5 5 2 0 unbiased 60.298992 0.857362 555.8153 1110.631 -0.0079 -7.1 -1.2999999999999998 8.56 2583.1 nuORFdb_human ENST00000311895.7_1_16:14015972-14016038:+ ENST00000311895.7_1_16:14015972-14016038:+ ERCC4 Out-of-Frame Out-of-Frame GN=ERCC4 Out-of-Frame E_20221201_NO30_400nL_HLAc1_2e7_directIP_titration_rep4_Slot2-4_1_3519 40mio-rep1 E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep1_Slot1-04_1_3496.17599.1402.2 2 1 1 18.29 9.82 9.32 8.47 87.5 8 1 1 7520.0 Hydrogen 8 (V) 9 VLYLRQVGY (L) 9 VLQLTFGIGY V/L/Y|L|R\Q|V|G\Y 100.0 9 6 6 4 0 dual b/y bias 60.791084 0.875087 555.8174 1110.631 -0.0039 -3.5 -0.2999999999999998 8.56 2583.1 nuORFdb_human ENST00000311895.7_1_16:14015972-14016038:+ ENST00000311895.7_1_16:14015972-14016038:+ ERCC4 Out-of-Frame Out-of-Frame GN=ERCC4 Out-of-Frame E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep1_Slot1-04_1_3496 40mio-rep2 E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep2_Slot2-5_1_3520.17362.1417.2 2 2 1 17.23 9.58 8.61 7.65 89.0 8 1 1 13100.0 Hydrogen 8 (V) 9 VLYLRQVGY (L) 9 VLQLTFGIGY V/L/Y|L|R\Q|V|G\Y 100.0 9 6 6 4 0 dual b/y bias 59.976551 0.865514 555.8168 1110.631 -0.0039 -3.5 1.4000000000000004 8.56 2583.1 nuORFdb_human ENST00000311895.7_1_16:14015972-14016038:+ ENST00000311895.7_1_16:14015972-14016038:+ ERCC4 Out-of-Frame Out-of-Frame GN=ERCC4 Out-of-Frame E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep2_Slot2-5_1_3520 40mio-rep3 E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep3_Slot2-5_1_3521.17385.1424.2 2 1 1 19.62 10.58 9.05 9.04 91.3 8 1 1 7220.0 Hydrogen 8 (V) 9 VLYLRQVGY (L) 9 VLQLTFGIGY V/L|Y|L|R\Q|V|G\Y 100.0 9 7 6 5 0 dual b/y bias 60.054527 0.861516 555.8157 1110.631 -0.0059 -5.3 -0.7000000000000002 8.56 2583.1 nuORFdb_human ENST00000311895.7_1_16:14015972-14016038:+ ENST00000311895.7_1_16:14015972-14016038:+ ERCC4 Out-of-Frame Out-of-Frame GN=ERCC4 Out-of-Frame E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep3_Slot2-5_1_3521 40mio-rep4 E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep4_Slot2-5_1_3522.17368.1418.2 2 1 1 14.94 9.85 6.81 5.09 84.6 8 1 1 5960.0 Hydrogen 8 (V) 9 VLYLRQVGY (L) 9 VLQLTFGIGY V/L|Y|L/R\Q\V\G\Y 100.0 9 6 4 2 0 unbiased 59.979736 0.865352 555.8154 1110.631 -0.0079 -7.1 -2.3999999999999995 8.56 2583.1 nuORFdb_human ENST00000311895.7_1_16:14015972-14016038:+ ENST00000311895.7_1_16:14015972-14016038:+ ERCC4 Out-of-Frame Out-of-Frame GN=ERCC4 Out-of-Frame E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep4_Slot2-5_1_3522 SCP E_20221201_NO30_400nL_HLAc1_4e7_PDAC_Slot2-6_1_3524.25028.513.1 1 3 1 10.72 6.57 5.22 4.16 85.3 6 0 0 34200.0 Hydrogen 60 (S) 8 VPFLVASY (I) 8 VQTVKASY V/P F|L\V|A\S\Y 75.0 6 5 3 2 0 unbiased 86.464363 1.417977 895.4899 895.492 -0.0024 -2.7 -0.30000000000000027 5.49 8575.8 smORF_v4|nuORFdb_human CTD-2555C10.3_14:103021059-103021289:+ CTD-2555C10.3_14:103021059-103021289:+|ENST00000559946.1_1_14:103016683-103021286:+ CTD-2555C10.3 lncRNA 14:103021059-103021289:+ 14 CTD-2555C10.3 lincRNA lncRNA GN=CTD-2555C10.3 E_20221201_NO30_400nL_HLAc1_4e7_PDAC_Slot2-6_1_3524 Eva-50mio-3fr AL20210827_EV_HLA_PDAC_PANFRO413_2D_50e6_W632_classI_fxn2.21608.21719.2 2 1 1 14.62 6.68 8.89 7.94 88.5 6 0 0 36100000.0 Hydrogen 66 (L) 9 VPHQPAPPF (F) 9 SARFPAPPF V P H\Q\P\A|P/P-F 66.7 6 4 2 1 1 b-ion bias 0.995 100.0 40.93 75.1 0.0 495.2639 989.52 0.0002 0.2 -0.09999999999999998 6.71 12786.7 nuORFdb_human ENST00000440918.5_1_20:37075363-37076627:+ ENST00000440918.5_1_20:37075363-37076627:+|ENST00000421599.5_1_20:37076141-37077369:+|ENST00000449351.5_1_20:37075363-37077305:+ SNHG11 lincRNA lncRNA GN=SNHG11 lincRNA AL20210827_EV_HLA_PDAC_PANFRO413_2D_50e6_W632_classI_fxn2 SCP E_20221201_NO30_400nL_HLAc1_4e7_PDAC_Slot2-6_1_3524.12525.1521.2 2 2 1 10.64 7.82 2.3 2.82 79.4 6 0 0 14500.0 Hydrogen 66 (L) 9 VPHQPAPPF (F) 9 SARFPAPPF V/P H Q/P\A|P|P\F 66.7 6 4 4 2 0 unbiased 43.269382 0.800001 495.2612 989.52 -0.0056 -5.7 -3.3000000000000003 6.71 12786.7 nuORFdb_human ENST00000440918.5_1_20:37075363-37076627:+ ENST00000440918.5_1_20:37075363-37076627:+|ENST00000421599.5_1_20:37076141-37077369:+|ENST00000449351.5_1_20:37075363-37077305:+ SNHG11 lincRNA lncRNA GN=SNHG11 lincRNA E_20221201_NO30_400nL_HLAc1_4e7_PDAC_Slot2-6_1_3524 Exploris_FAIMS VH20221207_MKP_FAIMS2022E_HLAc1_4e7_PDAC.33210.33210.2 2 1 1 13.69 5.73 2.38 7.96 89.0 6 0 0 9940000.0 Hydrogen 66 (L) 9 VPHQPAPPF (F) 9 SARFPAPPF V P H\Q\P\A|P/P-F 66.7 6 4 2 1 1 b-ion bias 0.892 100.0 63.5 74.13 -50.0 495.2624 989.52 -0.0028 -2.9 -0.2999999999999998 6.71 12786.7 nuORFdb_human ENST00000440918.5_1_20:37075363-37076627:+ ENST00000440918.5_1_20:37075363-37076627:+|ENST00000421599.5_1_20:37076141-37077369:+|ENST00000449351.5_1_20:37075363-37077305:+ SNHG11 lincRNA lncRNA GN=SNHG11 lincRNA VH20221207_MKP_FAIMS2022E_HLAc1_4e7_PDAC Eva-50mio-3fr AL20210827_EV_HLA_PDAC_PANFRO413_2D_50e6_W632_classI_fxn2.22784.22784.2 2 2 1 13.25 5.59 5.43 7.66 73.0 6 0 0 20400000.0 Hydrogen 238 (S) 9 VPHSIIDGY (K) 9 DLLHPSVGY V P H\S\I\I|D/G/Y 66.7 6 4 3 1 0 unbiased 0.658 100.0 43.53 100.0 0.0 500.7589 1000.51 0.0007 0.7 0.39999999999999997 5.07 34632.3 nuORFdb_human ENST00000496593.5_1_11:61404148-61405105:+ ENST00000496593.5_1_11:61404148-61405105:+ RPLP0P2 Pseudogene Pseudogene GN=RPLP0P2 Pseudogene AL20210827_EV_HLA_PDAC_PANFRO413_2D_50e6_W632_classI_fxn2 Exploris AL20221201_MKP_PDAC_01.16784.16891.2 2 1 1 13.43 9.57 4.22 3.86 89.4 7 0 1 86600000.0 Hydrogen 6 (K) 9 VPHTRPVSL (S) 9 VPSPHLRSL V/P|H/T/R P\V/S/L 77.8 7 2 6 1 0 y-ion bias 0.963 92.8 29.85 17.99 0.0 503.2967 1005.584 0.0022 2.2 1.4000000000000001 9.73 17668.9 nuORFdb_human ENST00000412609.5_1_19:40170059-40174276:+ ENST00000412609.5_1_19:40170059-40174276:+ LGALS17A Pseudogene Pseudogene GN=LGALS17A Pseudogene AL20221201_MKP_PDAC_01 SCP E_20221201_NO30_400nL_HLAc1_4e7_PDAC_Slot2-6_1_3524.7262.1457.2 2 2 1 13.61 8.33 4.14 5.28 86.6 6 0 1 56200.0 Hydrogen 6 (K) 9 VPHTRPVSL (S) 9 VPSPHLRSL V/P/H|T|R|P V\S L 55.6 5 4 5 3 0 dual b/y bias 25.091881 0.840672 503.2942 1005.584 -0.0033 -3.2 -0.8000000000000003 9.73 17668.9 nuORFdb_human ENST00000412609.5_1_19:40170059-40174276:+ ENST00000412609.5_1_19:40170059-40174276:+ LGALS17A Pseudogene Pseudogene GN=LGALS17A Pseudogene E_20221201_NO30_400nL_HLAc1_4e7_PDAC_Slot2-6_1_3524 Exploris_FAIMS VH20221207_MKP_FAIMS2022E_HLAc1_4e7_PDAC.21354.21458.2 2 1 1 15.93 10.13 5.26 5.8 90.8 8 0 1 73300000.0 Hydrogen 6 (K) 9 VPHTRPVSL (S) 9 VPSPHLRSL V/P|H/T/R\P\V/S/L 100.0 9 3 6 1 0 y-ion bias 0.999 100.0 44.8 27.1 -50.0 503.2935 1005.584 -0.0044 -4.3 -1.6999999999999997 9.73 17668.9 nuORFdb_human ENST00000412609.5_1_19:40170059-40174276:+ ENST00000412609.5_1_19:40170059-40174276:+ LGALS17A Pseudogene Pseudogene GN=LGALS17A Pseudogene VH20221207_MKP_FAIMS2022E_HLAc1_4e7_PDAC Eva-50mio-3fr AL20210827_EV_HLA_PDAC_PANFRO413_2D_50e6_W632_classI_fxn3.43986.43986.2 2 1 1 13.05 8.03 6.8 5.02 87.3 7 0 1 3590000.0 Hydrogen 49 (R) 9 VPIELFRPL (-) 9 VLPEFLRLP V/P|I/E|L/F/R/P-L 100.0 9 2 7 2 1 y-ion bias 0.999 67.7 79.58 100.0 0.0 542.3324 1083.656 0.0014 1.3 0.8 5.97 6480.3 nuORFdb_human ENST00000482361.1_1_6:41877193-41888846:- ENST00000482361.1_1_6:41877193-41888846:- MED20 5' Overlap uORF 5' Overlap uORF GN=MED20 5' Overlap uORF AL20210827_EV_HLA_PDAC_PANFRO413_2D_50e6_W632_classI_fxn3 Exploris_FAIMS VH20221207_MKP_FAIMS2022E_HLAc1_4e7_PDAC.62475.62475.2 2 1 1 12.96 7.91 5.74 5.05 85.4 7 0 1 7770000.0 Hydrogen 49 (R) 9 VPIELFRPL (-) 9 VLPEFLRLP V/P|I/E/L/F/R/P-L 100.0 9 1 7 1 1 y-ion bias 0.979 64.3 107.32 73.47 -70.0 542.33 1083.656 -0.0033 -3.1 -0.5 5.97 6480.3 nuORFdb_human ENST00000482361.1_1_6:41877193-41888846:- ENST00000482361.1_1_6:41877193-41888846:- MED20 5' Overlap uORF 5' Overlap uORF GN=MED20 5' Overlap uORF VH20221207_MKP_FAIMS2022E_HLAc1_4e7_PDAC SCP E_20221201_NO30_400nL_HLAc1_4e7_PDAC_Slot2-6_1_3524.25870.440.1 1 1 1 8.84 4.25 3.93 4.58 78.5 6 0 0 6070.0 Hydrogen 23 (R) 9 VPNANILVL (T) 9 VIANQPVVI V/P N A\N\I\L\V\L 66.7 6 5 1 0 0 b-ion bias 89.372963 1.461503 952.5793 952.583 -0.0036 -3.8 -1.4 5.49 5176.2 nuORFdb_human ENST00000293860.5_1_16:97482-101617:- ENST00000293860.5_1_16:97482-101617:- POLR3K Out-of-Frame Out-of-Frame GN=POLR3K Out-of-Frame E_20221201_NO30_400nL_HLAc1_4e7_PDAC_Slot2-6_1_3524 Exploris AL20221201_MKP_PDAC_01.11423.11423.2 2 2 1 9.04 5.99 3.95 3.05 63.4 6 1 2 42900000.0 Hydrogen 13 (S) 10 VPRKDVGNTL (H) 10 VPRTAAGGVGTL V/P R K D\V\G\N|T|L 60.0 6 5 3 2 0 unbiased 0.893 100.0 21.42 100.0 0.0 549.8155 1098.627 -0.0029 -2.6 -3.4000000000000004 8.72 2892.2 nuORFdb_human ENST00000493903.1_1_13:28004724-28006917:+ ENST00000493903.1_1_13:28004724-28006917:+ GTF3A ncRNA Processed Transcript lncRNA GN=GTF3A ncRNA Processed Transcript AL20221201_MKP_PDAC_01 SCP E_20221201_NO30_400nL_HLAc1_4e7_PDAC_Slot2-6_1_3524.4822.1281.2 2 1 1 12.4 10.04 6.1 2.37 79.1 7 1 2 7490.0 Hydrogen 13 (S) 10 VPRKDVGNTL (H) 10 VPRTAAGGVGTL V/P/R K D|V\G\N\T\L 70.0 7 5 3 1 0 unbiased 16.667555 0.95055 549.8146 1098.627 -0.0039 -3.5 -1.1 8.72 2892.2 nuORFdb_human ENST00000493903.1_1_13:28004724-28006917:+ ENST00000493903.1_1_13:28004724-28006917:+ GTF3A ncRNA Processed Transcript lncRNA GN=GTF3A ncRNA Processed Transcript E_20221201_NO30_400nL_HLAc1_4e7_PDAC_Slot2-6_1_3524 Eva-50mio-3fr AL20210827_EV_HLA_PDAC_PANFRO413_2D_50e6_W632_classI_fxn2.12227.12324.2 2 2 2 9.55 6.97 2.58 2.58 85.6 5 1 1 19600000.0 Hydrogen 32 (S) 9 VPTGCRTSL (-) 9 VPTVHHVSD V/P|T/G/C R T S/L 55.6 5 1 5 1 0 y-ion bias Carbamidomethylation C:Carbamidomethylation, 0.996 100.0 24.0 100.0 0.0 495.7558 990.504 0.0007 0.7 0.39999999999999997 9.72 4189.8 nuORFdb_human ENST00000534398.1_1_8:144824160-144824280:+ ENST00000534398.1_1_8:144824160-144824280:+ FAM83H-AS1 ncRNA Processed Transcript lncRNA GN=FAM83H-AS1 ncRNA Processed Transcript AL20210827_EV_HLA_PDAC_PANFRO413_2D_50e6_W632_classI_fxn2 Exploris AL20221201_MKP_PDAC_01.14883.15109.2 2 1 1 8.48 6.92 1.56 1.56 83.9 5 1 1 748000000.0 Hydrogen 32 (S) 9 VPTGCRTSL (-) 9 VPTVHHVSD V/P|T/G/C R T S-L 55.6 5 1 4 1 1 y-ion bias Carbamidomethylation C:Carbamidomethylation, 0.999 88.0 27.07 7.41 0.0 495.7557 990.504 0.0004 0.4 -0.4 9.72 4189.8 nuORFdb_human ENST00000534398.1_1_8:144824160-144824280:+ ENST00000534398.1_1_8:144824160-144824280:+ FAM83H-AS1 ncRNA Processed Transcript lncRNA GN=FAM83H-AS1 ncRNA Processed Transcript AL20221201_MKP_PDAC_01 SCP E_20221201_NO30_400nL_HLAc1_4e7_PDAC_Slot2-6_1_3524.6602.1462.2 2 2 1 15.38 12.2 3.68 3.18 94.0 6 1 1 423000.0 Hydrogen 32 (S) 9 VPTGCRTSL (-) 9 LSGCTEKPV V/P/T|G|C R T\S\L 66.7 6 4 4 2 0 unbiased Carbamidomethylation C:Carbamidomethylation, 22.813059 0.837245 495.7539 990.504 -0.003 -3.0 -0.6000000000000001 9.72 4189.8 nuORFdb_human ENST00000534398.1_1_8:144824160-144824280:+ ENST00000534398.1_1_8:144824160-144824280:+ FAM83H-AS1 ncRNA Processed Transcript lncRNA GN=FAM83H-AS1 ncRNA Processed Transcript E_20221201_NO30_400nL_HLAc1_4e7_PDAC_Slot2-6_1_3524 Exploris_FAIMS VH20221207_MKP_FAIMS2022E_HLAc1_4e7_PDAC.19525.19884.2 2 1 1 11.28 7.16 3.95 4.12 90.9 6 1 1 111000000.0 Hydrogen 32 (S) 9 VPTGCRTSL (-) 9 VPTVHHVSD V/P|T/G/C R T/S/L 66.7 6 1 6 1 0 y-ion bias Carbamidomethylation C:Carbamidomethylation, 0.994 95.9 41.5 57.58 -70.0 495.7542 990.504 -0.0027 -2.7 -0.10000000000000009 9.72 4189.8 nuORFdb_human ENST00000534398.1_1_8:144824160-144824280:+ ENST00000534398.1_1_8:144824160-144824280:+ FAM83H-AS1 ncRNA Processed Transcript lncRNA GN=FAM83H-AS1 ncRNA Processed Transcript VH20221207_MKP_FAIMS2022E_HLAc1_4e7_PDAC SCP E_20221201_NO30_400nL_HLAc1_4e7_PDAC_Slot2-6_1_3524.11053.1336.2 2 1 1 10.25 4.01 6.96 6.23 70.0 9 1 1 5440.0 Hydrogen 11 (A) 11 VPVGVARQPAY (H) 11 VHSAVITVPAY V/P/V/G/V/A/R Q|P-A\Y 81.8 9 2 7 1 1 y-ion bias 38.186069 0.916538 578.8267 1156.647 -0.0006 -0.5 1.9 8.72 12652.0 nuORFdb_human ENST00000409979.6_1_2:228336893-228337256:+ ENST00000409979.6_1_2:228336893-228337256:+ AGFG1 5' Overlap uORF 5' Overlap uORF GN=AGFG1 5' Overlap uORF E_20221201_NO30_400nL_HLAc1_4e7_PDAC_Slot2-6_1_3524 SCP E_20221201_NO30_400nL_HLAc1_4e7_PDAC_Slot2-6_1_3524.12874.1458.2 2 1 1 9.66 5.04 5.38 4.61 60.8 8 0 0 17200.0 Hydrogen 7 (P) 9 VPVQVAHEL (K) 9 VPVMGTKLF V/P/V/Q/V/A/H\E\L 100.0 9 2 6 0 0 y-ion bias 44.47747 0.840221 496.2813 991.557 -0.0024 -2.4 0.0 5.24 6937.9 nuORFdb_human ENST00000452173.2_1_10:81444487-81444691:- ENST00000452173.2_1_10:81444487-81444691:- RP11-119F19.4 Pseudogene Pseudogene GN=RP11-119F19.4 Pseudogene E_20221201_NO30_400nL_HLAc1_4e7_PDAC_Slot2-6_1_3524 Exploris_FAIMS VH20221207_MKP_FAIMS2022E_HLAc1_4e7_PDAC.34034.34034.2 2 1 1 17.06 10.57 10.12 6.49 86.9 8 0 0 22100000.0 Hydrogen 7 (P) 9 VPVQVAHEL (K) 9 VPQVHALnI V/P|V/Q|V/A/H\E-L 100.0 9 3 6 2 1 y-ion bias 0.998 97.6 64.78 54.79 -70.0 496.2809 991.557 -0.0026 -2.7 -0.10000000000000009 5.24 6937.9 nuORFdb_human ENST00000452173.2_1_10:81444487-81444691:- ENST00000452173.2_1_10:81444487-81444691:- RP11-119F19.4 Pseudogene Pseudogene GN=RP11-119F19.4 Pseudogene VH20221207_MKP_FAIMS2022E_HLAc1_4e7_PDAC Eva-50mio-3fr AL20210827_EV_HLA_PDAC_PANFRO413_2D_50e6_W632_classI_fxn2.34652.34652.1 1 1 1 8.75 3.14 2.45 5.6 81.3 7 0 0 1220000.0 Hydrogen 96 (E) 9 VPVTASAIL (-) 9 GATTVPLLV V P\V\T\A\S-A\I-L 77.8 7 5 0 0 2 b-ion bias 0.977 100.0 63.55 100.0 0.0 870.5307 870.529 0.0012 1.4 1.0999999999999999 5.49 11177.8 nuORFdb_human ENST00000313519.9_2_10:14920918-14938948:+ ENST00000313519.9_2_10:14920918-14938948:+|ENST00000420416.1_3_10:14920949-14938948:+ SUV39H2 5' Overlap uORF 5' Overlap uORF GN=SUV39H2 5' Overlap uORF AL20210827_EV_HLA_PDAC_PANFRO413_2D_50e6_W632_classI_fxn2 SCP E_20221201_NO30_400nL_HLAc1_4e7_PDAC_Slot2-6_1_3524.20837.578.1 1 2 1 11.35 7.84 2.58 3.51 79.9 6 0 0 27600.0 Hydrogen 96 (E) 9 VPVTASAIL (-) 9 VAPSLSALI V P V/T\A\S\A\I\L 66.7 6 5 1 0 0 b-ion bias 71.983543 1.379648 870.5289 870.529 -0.0005 -0.6 1.7999999999999998 5.49 11177.8 nuORFdb_human ENST00000313519.9_2_10:14920918-14938948:+ ENST00000313519.9_2_10:14920918-14938948:+|ENST00000420416.1_3_10:14920949-14938948:+ SUV39H2 5' Overlap uORF 5' Overlap uORF GN=SUV39H2 5' Overlap uORF E_20221201_NO30_400nL_HLAc1_4e7_PDAC_Slot2-6_1_3524 40mio-rep2 E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep2_Slot2-5_1_3520.18055.577.1 1 1 1 9.3 5.18 1.86 4.12 75.9 7 0 0 6470.0 Hydrogen 56 (E) 9 VQVGSVVTV (A) 9 VLQTQVSL V Q/V/G\S\V\V\T\V 77.8 7 5 2 0 0 b-ion bias 62.369835 1.379983 887.5154 887.52 -0.0047 -5.2 -0.2999999999999998 5.49 12523.8 nuORFdb_human ENST00000399922.6_1_14:23374642-23380538:- ENST00000399922.6_1_14:23374642-23380538:-|ENST00000557227.5_1_14:23374642-23375566:- RBM23 Out-of-Frame Out-of-Frame GN=RBM23 Out-of-Frame E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep2_Slot2-5_1_3520 40mio-rep4 E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep4_Slot2-5_1_3522.18063.570.1 1 1 1 9.21 5.79 2.22 3.42 77.7 7 0 0 3660.0 Hydrogen 56 (E) 9 VQVGSVVTV (A) 9 VDKAVGVSI V Q/V\G\S\V\V\T\V 77.8 7 6 1 0 0 b-ion bias 62.380833 1.384166 887.5169 887.52 -0.0027 -3.0 1.7000000000000002 5.49 12523.8 nuORFdb_human ENST00000399922.6_1_14:23374642-23380538:- ENST00000399922.6_1_14:23374642-23380538:-|ENST00000557227.5_1_14:23374642-23375566:- RBM23 Out-of-Frame Out-of-Frame GN=RBM23 Out-of-Frame E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep4_Slot2-5_1_3522 40mio-rep1 E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep1_Slot1-04_1_3496.10564.1532.2 2 3 1 10.19 7.21 2.98 2.98 86.6 6 1 1 23900.0 Hydrogen 37 (M) 8 VSAPRASW (R) 8 VSARGGAGGAA V/S/A|P/R A\S\W 75.0 6 3 4 1 0 unbiased 36.493492 0.79334 437.2304 873.458 -0.005 -5.7 -2.5 9.72 8620.5 nuORFdb_human ENST00000589688.1_1_17:40761735-40762498:+ ENST00000589688.1_1_17:40761735-40762498:+|ENST00000251412.7_1_17:40811887-40812224:+ TUBG1 5' Overlap uORF 5' Overlap uORF GN=TUBG1 5' Overlap uORF E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep1_Slot1-04_1_3496 40mio-rep4 E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep4_Slot2-5_1_3522.10307.1594.2 2 3 1 8.73 5.5 2.71 3.23 80.3 6 1 1 19600.0 Hydrogen 37 (M) 8 VSAPRASW (R) 8 VSAWRPGT V/S/A/P/R\A S\W 75.0 6 2 4 0 0 y-ion bias 35.596107 0.753919 437.2297 873.458 -0.005 -5.7 -1.0 9.72 8620.5 nuORFdb_human ENST00000589688.1_1_17:40761735-40762498:+ ENST00000589688.1_1_17:40761735-40762498:+|ENST00000251412.7_1_17:40811887-40812224:+ TUBG1 5' Overlap uORF 5' Overlap uORF GN=TUBG1 5' Overlap uORF E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep4_Slot2-5_1_3522 10mio-rep1 E_20221201_NO30_400nL_HLAc1_1e7_directIP_titration_rep1_Slot2-3_1_3512.13201.1529.2 2 1 1 7.1 3.28 4.43 3.82 66.6 8 1 1 3130.0 Hydrogen 29 (S) 9 VSARSLGSW (R) 9 ASVKDLGSW V/S/A/R\S-L\G\S\W 100.0 9 4 3 0 1 unbiased 45.606773 0.795248 481.7528 962.505 -0.0067 -6.9 0.7999999999999998 9.72 5938.6 nuORFdb_human ENST00000267984.3_1_15:81293385-81293562:+ ENST00000267984.3_1_15:81293385-81293562:+ MESDC1 5' uORF 5' uORF GN=MESDC1 5' uORF E_20221201_NO30_400nL_HLAc1_1e7_directIP_titration_rep1_Slot2-3_1_3512 10mio-rep2 E_20221201_NO30_400nL_HLAc1_1e7_directIP_titration_rep2_Slot2-3_1_3513.13168.1504.2 2 1 1 10.16 6.59 3.58 3.58 74.1 6 1 1 4130.0 Hydrogen 29 (S) 9 VSARSLGSW (R) 9 VSLSAGRSW V/S/A/R S L|G|S\W 66.7 6 3 5 2 0 unbiased 45.492027 0.810765 481.751 962.505 -0.0107 -11.1 -4.3999999999999995 9.72 5938.6 nuORFdb_human ENST00000267984.3_1_15:81293385-81293562:+ ENST00000267984.3_1_15:81293385-81293562:+ MESDC1 5' uORF 5' uORF GN=MESDC1 5' uORF E_20221201_NO30_400nL_HLAc1_1e7_directIP_titration_rep2_Slot2-3_1_3513 20mio-rep1 E_20221201_NO30_400nL_HLAc1_2e7_directIP_titration_rep1_Slot2-4_1_3515.13091.1512.2 2 2 1 9.35 6.53 3.36 2.82 71.0 6 1 1 16300.0 Hydrogen 29 (S) 9 VSARSLGSW (R) 9 VSLSAGRSW V/S/A R S\L|G|S\W 66.7 6 4 4 2 0 unbiased 45.215595 0.806145 481.7534 962.505 -0.0067 -6.9 -0.7000000000000002 9.72 5938.6 nuORFdb_human ENST00000267984.3_1_15:81293385-81293562:+ ENST00000267984.3_1_15:81293385-81293562:+ MESDC1 5' uORF 5' uORF GN=MESDC1 5' uORF E_20221201_NO30_400nL_HLAc1_2e7_directIP_titration_rep1_Slot2-4_1_3515 20mio-rep2 E_20221201_NO30_400nL_HLAc1_2e7_directIP_titration_rep3_Slot2-4_1_3518.13228.1511.2 2 3 1 10.76 6.61 3.79 4.15 82.5 7 1 1 15300.0 Hydrogen 29 (S) 9 VSARSLGSW (R) 9 ASVKDLGSW V/S/A/R S|L|G|S\W 77.8 7 4 6 3 0 dual b/y bias 45.690144 0.806288 481.7528 962.505 -0.0067 -6.9 -0.6000000000000005 9.72 5938.6 nuORFdb_human ENST00000267984.3_1_15:81293385-81293562:+ ENST00000267984.3_1_15:81293385-81293562:+ MESDC1 5' uORF 5' uORF GN=MESDC1 5' uORF E_20221201_NO30_400nL_HLAc1_2e7_directIP_titration_rep3_Slot2-4_1_3518 20mio-rep3 E_20221201_NO30_400nL_HLAc1_2e7_directIP_titration_rep4_Slot2-4_1_3519.13124.1514.2 2 1 1 9.54 7.21 2.33 2.33 76.9 5 1 1 3360.0 Hydrogen 29 (S) 9 VSARSLGSW (R) 9 VSLSAGRSW V/S/A R S L|G|S\W 55.6 5 3 4 2 0 unbiased 45.334469 0.804991 481.7532 962.505 -0.0067 -6.9 -1.1000000000000005 9.72 5938.6 nuORFdb_human ENST00000267984.3_1_15:81293385-81293562:+ ENST00000267984.3_1_15:81293385-81293562:+ MESDC1 5' uORF 5' uORF GN=MESDC1 5' uORF E_20221201_NO30_400nL_HLAc1_2e7_directIP_titration_rep4_Slot2-4_1_3519 40mio-rep1 E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep1_Slot1-04_1_3496.13379.1480.2 2 3 1 15.71 8.91 7.54 6.8 90.7 7 1 1 29900.0 Hydrogen 29 (S) 9 VSARSLGSW (R) 9 RGMRLGSW V/S/A R|S|L|G|S\W 77.8 7 5 6 4 0 dual b/y bias 46.21542 0.826308 481.7543 962.505 -0.0047 -4.9 -1.7000000000000002 9.72 5938.6 nuORFdb_human ENST00000267984.3_1_15:81293385-81293562:+ ENST00000267984.3_1_15:81293385-81293562:+ MESDC1 5' uORF 5' uORF GN=MESDC1 5' uORF E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep1_Slot1-04_1_3496 40mio-rep2 E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep2_Slot2-5_1_3520.13084.1503.2 2 2 1 10.74 7.71 1.41 3.03 82.4 7 1 1 17300.0 Hydrogen 29 (S) 9 VSARSLGSW (R) 9 AVDKSIGSW V/S/A/R S/L|G|S\W 77.8 7 3 6 2 0 y-ion bias 45.198818 0.811356 481.7534 962.505 -0.0067 -6.9 -2.0 9.72 5938.6 nuORFdb_human ENST00000267984.3_1_15:81293385-81293562:+ ENST00000267984.3_1_15:81293385-81293562:+ MESDC1 5' uORF 5' uORF GN=MESDC1 5' uORF E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep2_Slot2-5_1_3520 40mio-rep3 E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep3_Slot2-5_1_3521.13093.1533.2 2 2 1 11.95 6.82 5.87 5.13 77.7 8 1 1 21000.0 Hydrogen 29 (S) 9 VSARSLGSW (R) 9 ASVKDLGSW V/S/A/R\S\L|G|S\W 100.0 9 5 5 2 0 unbiased 45.233608 0.792964 481.7534 962.505 -0.0067 -6.9 -2.3000000000000007 9.72 5938.6 nuORFdb_human ENST00000267984.3_1_15:81293385-81293562:+ ENST00000267984.3_1_15:81293385-81293562:+ MESDC1 5' uORF 5' uORF GN=MESDC1 5' uORF E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep3_Slot2-5_1_3521 40mio-rep4 E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep4_Slot2-5_1_3522.13081.1503.2 2 1 1 13.19 6.22 6.48 6.97 84.1 8 1 1 13900.0 Hydrogen 29 (S) 9 VSARSLGSW (R) 9 SEKRIGSW V/S/A/R|S\L|G|S\W 100.0 9 5 6 3 0 dual b/y bias 45.172615 0.811753 481.7544 962.505 -0.0047 -4.9 -0.20000000000000018 9.72 5938.6 nuORFdb_human ENST00000267984.3_1_15:81293385-81293562:+ ENST00000267984.3_1_15:81293385-81293562:+ MESDC1 5' uORF 5' uORF GN=MESDC1 5' uORF E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep4_Slot2-5_1_3522 40mio-rep4 E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep4_Slot2-5_1_3522.25109.420.1 1 3 1 7.98 2.79 4.06 5.19 75.7 6 0 0 8480.0 Hydrogen 6 (S) 9 VSDPLQTVW (L) 9 GFVTPLQGPE V S/D P\L|Q\T\V\W 55.6 5 5 2 1 0 b-ion bias 86.726997 1.473444 1044.532 1044.536 -0.006 -5.8 -1.0999999999999996 3.8 3374.9 nuORFdb_human ENST00000541490.5_1_12:26217565-26217655:+ ENST00000541490.5_1_12:26217565-26217655:+ RASSF8 Out-of-Frame Out-of-Frame GN=RASSF8 Out-of-Frame E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep4_Slot2-5_1_3522 20mio-rep1 E_20221201_NO30_400nL_HLAc1_2e7_directIP_titration_rep1_Slot2-4_1_3515.20756.1457.2 2 1 1 12.46 9.13 3.35 3.33 79.6 7 0 0 6740.0 Hydrogen 9 (Q) 9 VSFVHSISL (C) 9 VDPTPFLSL V/S/F/V/H S\I|S\L 77.8 7 3 5 1 0 unbiased 71.678619 0.840665 494.7738 988.546 -0.0055 -5.5 0.7000000000000002 6.71 2563.9 nuORFdb_human ENST00000489742.1_1_2:47397901-47403439:- ENST00000489742.1_1_2:47397901-47403439:-|ENST00000456319.5_2_2:47397898-47403621:- CALM2 5' Overlap uORF 5' Overlap uORF GN=CALM2 5' Overlap uORF E_20221201_NO30_400nL_HLAc1_2e7_directIP_titration_rep1_Slot2-4_1_3515 20mio-rep3 E_20221201_NO30_400nL_HLAc1_2e7_directIP_titration_rep4_Slot2-4_1_3519.20817.1458.2 2 1 1 11.98 7.02 4.97 4.96 84.4 7 0 0 5050.0 Hydrogen 9 (Q) 9 VSFVHSISL (C) 9 VFLPTPDSL V/S/F/V/H S\I\S\L 77.8 7 3 4 0 0 unbiased 71.898346 0.840007 494.7724 988.546 -0.0095 -9.6 -3.8 6.71 2563.9 nuORFdb_human ENST00000489742.1_1_2:47397901-47403439:- ENST00000489742.1_1_2:47397901-47403439:-|ENST00000456319.5_2_2:47397898-47403621:- CALM2 5' Overlap uORF 5' Overlap uORF GN=CALM2 5' Overlap uORF E_20221201_NO30_400nL_HLAc1_2e7_directIP_titration_rep4_Slot2-4_1_3519 40mio-rep1 E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep1_Slot1-04_1_3496.21005.1436.2 2 2 1 11.61 4.59 6.79 7.02 81.6 8 0 0 20500.0 Hydrogen 9 (Q) 9 VSFVHSISL (C) 9 SVVFHSTVI V/S/F/V/H\S-I\S\L 100.0 9 3 4 0 1 unbiased 72.562088 0.853475 494.7758 988.546 -0.0015 -1.5 1.7000000000000002 6.71 2563.9 nuORFdb_human ENST00000489742.1_1_2:47397901-47403439:- ENST00000489742.1_1_2:47397901-47403439:-|ENST00000456319.5_2_2:47397898-47403621:- CALM2 5' Overlap uORF 5' Overlap uORF GN=CALM2 5' Overlap uORF E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep1_Slot1-04_1_3496 40mio-rep2 E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep2_Slot2-5_1_3520.20852.1447.2 2 2 1 14.85 10.24 6.39 4.61 88.1 8 0 0 13600.0 Hydrogen 9 (Q) 9 VSFVHSISL (C) 9 SVVFHSTVI V/S/F/V/H|S\I\S\L 100.0 9 4 5 1 0 unbiased 72.035667 0.846653 494.7742 988.546 -0.0055 -5.5 -0.5999999999999996 6.71 2563.9 nuORFdb_human ENST00000489742.1_1_2:47397901-47403439:- ENST00000489742.1_1_2:47397901-47403439:-|ENST00000456319.5_2_2:47397898-47403621:- CALM2 5' Overlap uORF 5' Overlap uORF GN=CALM2 5' Overlap uORF E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep2_Slot2-5_1_3520 40mio-rep3 E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep3_Slot2-5_1_3521.20878.1453.2 2 2 1 15.36 10.2 5.35 5.16 88.3 8 0 0 11200.0 Hydrogen 9 (Q) 9 VSFVHSISL (C) 9 VSGLAAnRSL V/S/F/V/H\S\I\S\L 100.0 9 4 4 0 0 unbiased 72.122665 0.842847 494.7746 988.546 -0.0035 -3.5 1.0999999999999996 6.71 2563.9 nuORFdb_human ENST00000489742.1_1_2:47397901-47403439:- ENST00000489742.1_1_2:47397901-47403439:-|ENST00000456319.5_2_2:47397898-47403621:- CALM2 5' Overlap uORF 5' Overlap uORF GN=CALM2 5' Overlap uORF E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep3_Slot2-5_1_3521 40mio-rep4 E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep4_Slot2-5_1_3522.20877.1451.2 2 4 1 13.91 9.01 4.2 4.9 86.3 8 0 0 22100.0 Hydrogen 9 (Q) 9 VSFVHSISL (C) 9 VDPTPFLSL V/S/F/V/H\S\I\S\L 100.0 9 4 4 0 0 unbiased 72.101944 0.844224 494.7736 988.546 -0.0055 -5.5 -0.7999999999999998 6.71 2563.9 nuORFdb_human ENST00000489742.1_1_2:47397901-47403439:- ENST00000489742.1_1_2:47397901-47403439:-|ENST00000456319.5_2_2:47397898-47403621:- CALM2 5' Overlap uORF 5' Overlap uORF GN=CALM2 5' Overlap uORF E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep4_Slot2-5_1_3522 10mio-rep1 E_20221201_NO30_400nL_HLAc1_1e7_directIP_titration_rep1_Slot2-3_1_3512.14855.1476.2 2 2 1 14.0 8.94 5.05 5.05 88.3 8 1 1 10800.0 Hydrogen 13 (A) 9 VSNLVSRAW (-) 9 VSNIKPLCT V/S/N|L|V/S/R\A\W 100.0 9 4 6 2 0 unbiased 51.319088 0.828521 516.2828 1031.563 -0.0045 -4.4 3.3 9.72 2415.9 nuORFdb_human ENST00000343677.3_1_6:26056391-26056457:- ENST00000343677.3_1_6:26056391-26056457:- HIST1H1C Out-of-Frame Out-of-Frame GN=HIST1H1C Out-of-Frame E_20221201_NO30_400nL_HLAc1_1e7_directIP_titration_rep1_Slot2-3_1_3512 10mio-rep2 E_20221201_NO30_400nL_HLAc1_1e7_directIP_titration_rep2_Slot2-3_1_3513.14815.1472.2 2 3 1 15.85 8.0 5.27 7.85 87.4 8 1 1 14700.0 Hydrogen 13 (A) 9 VSNLVSRAW (-) 9 VSNLWTRVG V/S/N|L|V/S|R|A\W 100.0 9 5 7 4 0 dual b/y bias 51.17844 0.830872 516.2814 1031.563 -0.0085 -8.3 -1.6000000000000005 9.72 2415.9 nuORFdb_human ENST00000343677.3_1_6:26056391-26056457:- ENST00000343677.3_1_6:26056391-26056457:- HIST1H1C Out-of-Frame Out-of-Frame GN=HIST1H1C Out-of-Frame E_20221201_NO30_400nL_HLAc1_1e7_directIP_titration_rep2_Slot2-3_1_3513 10mio-rep3 E_20221201_NO30_400nL_HLAc1_1e7_directIP_titration_rep3_Slot2-3_1_3514.14755.1476.2 2 2 1 13.58 7.8 4.73 5.78 85.0 8 1 1 11100.0 Hydrogen 13 (A) 9 VSNLVSRAW (-) 9 VSNIKPLCT V/S/N|L|V/S/R\A\W 100.0 9 4 6 2 0 unbiased 50.973553 0.82838 516.2827 1031.563 -0.0045 -4.4 1.8999999999999995 9.72 2415.9 nuORFdb_human ENST00000343677.3_1_6:26056391-26056457:- ENST00000343677.3_1_6:26056391-26056457:- HIST1H1C Out-of-Frame Out-of-Frame GN=HIST1H1C Out-of-Frame E_20221201_NO30_400nL_HLAc1_1e7_directIP_titration_rep3_Slot2-3_1_3514 20mio-rep1 E_20221201_NO30_400nL_HLAc1_2e7_directIP_titration_rep1_Slot2-4_1_3515.14716.1465.2 2 3 1 14.44 7.55 6.15 6.89 87.7 8 1 1 19000.0 Hydrogen 13 (A) 9 VSNLVSRAW (-) 9 VSNIKPLCT V/S/N|L|V/S/R|A\W 100.0 9 4 7 3 0 dual b/y bias 50.826042 0.835788 516.2815 1031.563 -0.0085 -8.3 -2.1000000000000005 9.72 2415.9 nuORFdb_human ENST00000343677.3_1_6:26056391-26056457:- ENST00000343677.3_1_6:26056391-26056457:- HIST1H1C Out-of-Frame Out-of-Frame GN=HIST1H1C Out-of-Frame E_20221201_NO30_400nL_HLAc1_2e7_directIP_titration_rep1_Slot2-4_1_3515 20mio-rep2 E_20221201_NO30_400nL_HLAc1_2e7_directIP_titration_rep3_Slot2-4_1_3518.14824.1466.2 2 2 1 11.08 6.88 4.73 4.2 80.4 7 1 1 16000.0 Hydrogen 13 (A) 9 VSNLVSRAW (-) 9 VSNLWTRVG V/S/N|L|V/S/R A\W 77.8 7 3 6 2 0 y-ion bias 51.203293 0.834693 516.2824 1031.563 -0.0065 -6.3 0.0 9.72 2415.9 nuORFdb_human ENST00000343677.3_1_6:26056391-26056457:- ENST00000343677.3_1_6:26056391-26056457:- HIST1H1C Out-of-Frame Out-of-Frame GN=HIST1H1C Out-of-Frame E_20221201_NO30_400nL_HLAc1_2e7_directIP_titration_rep3_Slot2-4_1_3518 20mio-rep3 E_20221201_NO30_400nL_HLAc1_2e7_directIP_titration_rep4_Slot2-4_1_3519.14761.1468.2 2 2 1 14.66 8.73 5.55 5.93 87.4 8 1 1 15000.0 Hydrogen 13 (A) 9 VSNLVSRAW (-) 9 VSNLWTRVG V/S/N|L|V/S|R\A\W 100.0 9 5 6 3 0 dual b/y bias 50.98505 0.833815 516.2816 1031.563 -0.0065 -6.3 -0.5 9.72 2415.9 nuORFdb_human ENST00000343677.3_1_6:26056391-26056457:- ENST00000343677.3_1_6:26056391-26056457:- HIST1H1C Out-of-Frame Out-of-Frame GN=HIST1H1C Out-of-Frame E_20221201_NO30_400nL_HLAc1_2e7_directIP_titration_rep4_Slot2-4_1_3519 40mio-rep1 E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep1_Slot1-04_1_3496.14996.1444.2 2 1 1 13.33 7.34 5.63 5.99 81.0 8 1 1 19000.0 Hydrogen 13 (A) 9 VSNLVSRAW (-) 9 VSNIKPLCT V/S/N|L|V/S/R\A\W 100.0 9 4 6 2 0 unbiased 51.800613 0.848497 516.2838 1031.563 -0.0025 -2.5 0.7000000000000002 9.72 2415.9 nuORFdb_human ENST00000343677.3_1_6:26056391-26056457:- ENST00000343677.3_1_6:26056391-26056457:- HIST1H1C Out-of-Frame Out-of-Frame GN=HIST1H1C Out-of-Frame E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep1_Slot1-04_1_3496 40mio-rep2 E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep2_Slot2-5_1_3520.14699.1462.2 2 1 1 14.18 9.29 5.44 4.9 80.5 8 1 1 16100.0 Hydrogen 13 (A) 9 VSNLVSRAW (-) 9 VSNLWTRVG V/S/N|L|V/S/R/A\W 100.0 9 3 7 2 0 y-ion bias 50.776405 0.837361 516.2833 1031.563 -0.0045 -4.4 0.5 9.72 2415.9 nuORFdb_human ENST00000343677.3_1_6:26056391-26056457:- ENST00000343677.3_1_6:26056391-26056457:- HIST1H1C Out-of-Frame Out-of-Frame GN=HIST1H1C Out-of-Frame E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep2_Slot2-5_1_3520 40mio-rep3 E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep3_Slot2-5_1_3521.14716.1471.2 2 1 1 14.34 7.31 6.89 7.03 86.8 8 1 1 8280.0 Hydrogen 13 (A) 9 VSNLVSRAW (-) 9 VSNIKPLCT V/S/N|L|V/S/R\A\W 100.0 9 4 6 2 0 unbiased 50.837631 0.831597 516.2835 1031.563 -0.0045 -4.4 0.1999999999999993 9.72 2415.9 nuORFdb_human ENST00000343677.3_1_6:26056391-26056457:- ENST00000343677.3_1_6:26056391-26056457:- HIST1H1C Out-of-Frame Out-of-Frame GN=HIST1H1C Out-of-Frame E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep3_Slot2-5_1_3521 40mio-rep4 E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep4_Slot2-5_1_3522.14701.1466.2 2 1 1 14.2 7.52 5.56 6.68 83.9 8 1 1 12100.0 Hydrogen 13 (A) 9 VSNLVSRAW (-) 9 VSNIKPLCT V/S/N|L|V/S/R|A\W 100.0 9 4 7 3 0 dual b/y bias 50.771423 0.834709 516.2834 1031.563 -0.0045 -4.4 0.2999999999999998 9.72 2415.9 nuORFdb_human ENST00000343677.3_1_6:26056391-26056457:- ENST00000343677.3_1_6:26056391-26056457:- HIST1H1C Out-of-Frame Out-of-Frame GN=HIST1H1C Out-of-Frame E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep4_Slot2-5_1_3522 Exploris AL20221201_MKP_PDAC_01.15729.15729.2 2 2 1 7.64 4.72 3.02 2.92 62.2 6 2 2 65800000.0 Hydrogen 5 (L) 9 VSRVLKSTV (R) 9 VSRVLKSTV|RLATLKSTV SGQKKIKTV V/S R V\L\K\S|T\V 66.7 6 5 2 1 0 b-ion bias 0.949 61.3 28.28 100.0 0.0 494.8117 988.615 0.0011 1.1 0.30000000000000004 11.0 1772.2 nuORFdb_human ENST00000395003.5_2_5:133861893-133861941:+ ENST00000395003.5_2_5:133861893-133861941:+|ENST00000483373.5_1_3:142315288-142364209:+|ENST00000495744.5_1_3:142315288-142364209:+ JADE2 5' uORF 5' uORF GN=JADE2 5' uORF AL20221201_MKP_PDAC_01 SCP E_20221201_NO30_400nL_HLAc1_4e7_PDAC_Slot2-6_1_3524.6404.1434.2 2 1 1 11.92 7.99 3.69 3.93 76.8 6 2 2 57000.0 Hydrogen 5 (L) 9 VSRVLKSTV (R) 9 VSRVLKSTV|RLATLKSTV KQKGLKSLS V/S R V|L|K|S\T\V 66.7 6 5 4 3 0 dual b/y bias 22.129053 0.854825 494.8089 988.615 -0.0042 -4.3 -1.9 11.0 1772.2 nuORFdb_human ENST00000395003.5_2_5:133861893-133861941:+ ENST00000395003.5_2_5:133861893-133861941:+|ENST00000483373.5_1_3:142315288-142364209:+|ENST00000495744.5_1_3:142315288-142364209:+ JADE2 5' uORF 5' uORF GN=JADE2 5' uORF E_20221201_NO30_400nL_HLAc1_4e7_PDAC_Slot2-6_1_3524 40mio-rep1 E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep1_Slot1-04_1_3496.6700.1434.2 2 2 1 9.05 7.62 4.74 1.43 72.3 6 2 2 20100.0 Hydrogen 5 (L) 9 VSRVLKSTV (R) 9 RLATLKSTV V/S R V\L|K|S\T\V 66.7 6 5 3 2 0 unbiased 23.152611 0.854747 494.8086 988.615 -0.0042 -4.3 -1.0999999999999996 11.0 1772.2 nuORFdb_human ENST00000395003.5_2_5:133861893-133861941:+ ENST00000395003.5_2_5:133861893-133861941:+ JADE2 5' uORF 5' uORF GN=JADE2 5' uORF E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep1_Slot1-04_1_3496 40mio-rep2 E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep2_Slot2-5_1_3520.6403.1450.2 2 1 1 10.12 5.99 3.15 4.12 74.4 6 2 2 4370.0 Hydrogen 5 (L) 9 VSRVLKSTV (R) 9 VSRVLKSTV|RLATLKSTV SGQKKIKTV V/S R V\L|K\S\T\V 66.7 6 5 2 1 0 b-ion bias 22.126261 0.844962 494.8086 988.615 -0.0042 -4.3 0.6000000000000005 11.0 1772.2 nuORFdb_human ENST00000395003.5_2_5:133861893-133861941:+ ENST00000395003.5_2_5:133861893-133861941:+|ENST00000483373.5_1_3:142315288-142364209:+|ENST00000495744.5_1_3:142315288-142364209:+ JADE2 5' uORF 5' uORF GN=JADE2 5' uORF E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep2_Slot2-5_1_3520 Exploris_FAIMS VH20221207_MKP_FAIMS2022E_HLAc1_4e7_PDAC.20080.20189.2 2 1 1 11.62 7.76 4.17 3.86 76.4 6 2 2 13300000.0 Hydrogen 5 (L) 9 VSRVLKSTV (R) 9 VSRVLKSTV|RLATLKSTV SGQKKIKTV V/S R V\L\K\S|T|V 66.7 6 5 3 2 0 unbiased 0.998 94.3 42.38 27.58 -70.0 494.8096 988.615 -0.0031 -3.1 -0.5 11.0 1772.2 nuORFdb_human ENST00000395003.5_2_5:133861893-133861941:+ ENST00000395003.5_2_5:133861893-133861941:+|ENST00000483373.5_1_3:142315288-142364209:+|ENST00000495744.5_1_3:142315288-142364209:+ JADE2 5' uORF 5' uORF GN=JADE2 5' uORF VH20221207_MKP_FAIMS2022E_HLAc1_4e7_PDAC 40mio-rep1 E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep1_Slot1-04_1_3496.9455.1378.2 2 1 1 13.5 8.86 8.33 4.65 84.4 7 1 2 12700.0 Hydrogen 31 (H) 9 VSVNVRKTF (S) 9 VSVNKRISF V/S/V/N/V/R K|T\F 77.8 7 2 6 1 0 y-ion bias 32.664486 0.889827 525.3075 1049.61 -0.0035 -3.3 -0.09999999999999964 11.0 5659.3 nuORFdb_human ENST00000396836.6_1_5:55147345-55155373:+ ENST00000396836.6_1_5:55147345-55155373:+ IL31RA 5' Overlap uORF 5' Overlap uORF GN=IL31RA 5' Overlap uORF E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep1_Slot1-04_1_3496 40mio-rep3 E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep3_Slot2-5_1_3521.19237.713.1 1 1 1 8.12 3.93 5.99 4.19 67.3 6 0 0 3020.0 Hydrogen 115 (W) 8 VTATGLGW (A) 8 LSAAVTGW V T/A/T|G\L\G\W 75.0 6 4 3 1 0 unbiased 66.452072 1.298191 804.4247 804.425 -0.0 -0.0 4.6 5.49 15148.2 nuORFdb_human ENST00000525484.5_1_11:8986226-8986655:+ ENST00000525484.5_1_11:8986226-8986655:+ TMEM9B-AS1 Antisense lncRNA GN=TMEM9B-AS1 Antisense E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep3_Slot2-5_1_3521 10mio-rep3 E_20221201_NO30_400nL_HLAc1_1e7_directIP_titration_rep3_Slot2-3_1_3514.7239.1470.2 2 1 1 10.4 10.4 10.4 57.7 8 0 0 2120.0 Hydrogen 22 (A) 9 VTDQASHIY (-) 9 V/T/D/Q/A-S|H\I\Y 100.0 9 3 5 1 1 unbiased 25.014673 0.832343 517.2498 1033.495 -0.0022 -2.1 4.199999999999999 5.07 3559.0 nuORFdb_human ENST00000515608.5_1_8:6484738-6484828:- ENST00000515608.5_1_8:6484738-6484828:- MCPH1-AS1 Antisense lncRNA GN=MCPH1-AS1 Antisense E_20221201_NO30_400nL_HLAc1_1e7_directIP_titration_rep3_Slot2-3_1_3514 20mio-rep1 E_20221201_NO30_400nL_HLAc1_2e7_directIP_titration_rep1_Slot2-4_1_3515.7162.1449.2 2 2 1 7.41 4.13 2.7 3.28 62.7 6 0 0 7590.0 Hydrogen 22 (A) 9 VTDQASHIY (-) 9 VTHSFISAGn V/T/D/Q/A S H-I\Y 66.7 6 1 4 0 1 y-ion bias 24.743813 0.845661 517.2485 1033.495 -0.0062 -6.0 0.20000000000000018 5.07 3559.0 nuORFdb_human ENST00000515608.5_1_8:6484738-6484828:- ENST00000515608.5_1_8:6484738-6484828:- MCPH1-AS1 Antisense lncRNA GN=MCPH1-AS1 Antisense E_20221201_NO30_400nL_HLAc1_2e7_directIP_titration_rep1_Slot2-4_1_3515 20mio-rep3 E_20221201_NO30_400nL_HLAc1_2e7_directIP_titration_rep4_Slot2-4_1_3519.7203.1453.2 2 1 1 12.22 8.25 7.16 3.97 74.4 7 0 0 5580.0 Hydrogen 22 (A) 9 VTDQASHIY (-) 9 VSQETGHLY V/T/D/Q|A S/H\I\Y 77.8 7 3 5 1 0 unbiased 24.883631 0.842936 517.247 1033.495 -0.0082 -7.9 -2.1000000000000005 5.07 3559.0 nuORFdb_human ENST00000515608.5_1_8:6484738-6484828:- ENST00000515608.5_1_8:6484738-6484828:- MCPH1-AS1 Antisense lncRNA GN=MCPH1-AS1 Antisense E_20221201_NO30_400nL_HLAc1_2e7_directIP_titration_rep4_Slot2-4_1_3519 40mio-rep1 E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep1_Slot1-04_1_3496.7463.1428.2 2 2 1 13.75 8.12 6.82 5.63 82.7 8 0 0 20200.0 Hydrogen 22 (A) 9 VTDQASHIY (-) 9 VTnGAGDDKR V/T/D|Q|A-S/H\I\Y 100.0 9 4 5 2 1 unbiased 25.787046 0.858565 517.2491 1033.495 -0.0042 -4.0 -0.7999999999999998 5.07 3559.0 nuORFdb_human ENST00000515608.5_1_8:6484738-6484828:- ENST00000515608.5_1_8:6484738-6484828:- MCPH1-AS1 Antisense lncRNA GN=MCPH1-AS1 Antisense E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep1_Slot1-04_1_3496 40mio-rep2 E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep2_Slot2-5_1_3520.7165.1437.2 2 1 1 15.1 10.12 9.35 4.97 88.4 8 0 0 12400.0 Hydrogen 22 (A) 9 VTDQASHIY (-) 9 VSQETGHLY V/T/D/Q|A-S|H\I\Y 100.0 9 4 5 2 1 unbiased 24.75736 0.853249 517.2476 1033.495 -0.0062 -6.0 -1.0999999999999996 5.07 3559.0 nuORFdb_human ENST00000515608.5_1_8:6484738-6484828:- ENST00000515608.5_1_8:6484738-6484828:- MCPH1-AS1 Antisense lncRNA GN=MCPH1-AS1 Antisense E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep2_Slot2-5_1_3520 40mio-rep3 E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep3_Slot2-5_1_3521.7176.1440.2 2 1 1 14.63 8.33 6.96 6.29 87.9 7 0 0 12900.0 Hydrogen 22 (A) 9 VTDQASHIY (-) 9 VSQETGHLY V/T/D/Q|A S/H\I\Y 77.8 7 3 5 1 0 unbiased 24.791225 0.851371 517.2478 1033.495 -0.0062 -6.0 -1.4000000000000004 5.07 3559.0 nuORFdb_human ENST00000515608.5_1_8:6484738-6484828:- ENST00000515608.5_1_8:6484738-6484828:- MCPH1-AS1 Antisense lncRNA GN=MCPH1-AS1 Antisense E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep3_Slot2-5_1_3521 40mio-rep4 E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep4_Slot2-5_1_3522.7176.1438.2 2 1 1 13.74 8.08 6.4 5.66 83.4 7 0 0 11800.0 Hydrogen 22 (A) 9 VTDQASHIY (-) 9 VSQETGHLY V/T/D/Q|A S/H\I\Y 77.8 7 3 5 1 0 unbiased 24.787836 0.852739 517.2489 1033.495 -0.0042 -4.0 0.7000000000000002 5.07 3559.0 nuORFdb_human ENST00000515608.5_1_8:6484738-6484828:- ENST00000515608.5_1_8:6484738-6484828:- MCPH1-AS1 Antisense lncRNA GN=MCPH1-AS1 Antisense E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep4_Slot2-5_1_3522 40mio-rep4 E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep4_Slot2-5_1_3522.9493.1434.2 2 1 1 9.86 7.84 4.72 2.02 64.7 7 0 0 5570.0 Hydrogen 86 (A) 11 VTGPHAAASTW (S) 11 FGTSPRTQFG V T/G P H\A\A\A|S|T\W 54.5 6 6 3 2 0 b-ion bias 32.786247 0.854771 549.2685 1097.537 -0.0087 -7.9 -3.2 6.71 14167.7 nuORFdb_human ENST00000360694.7_1_6:35378793-35389621:+ ENST00000360694.7_1_6:35378793-35389621:+ PPARD 5' Overlap uORF 5' Overlap uORF GN=PPARD 5' Overlap uORF E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep4_Slot2-5_1_3522 40mio-rep1 E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep1_Slot1-04_1_3496.5183.1600.3 3 3 1 12.12 2.92 7.97 9.2 73.4 8 2 3 61200.0 Hydrogen 33 (L) 10 VTHKKIAVRW (-) 10 VTKPVPAGGLLSV V/T/H/K/K|I|A\V-R\W 100.0 10 4 6 2 1 unbiased 17.915417 0.750165 413.254 1237.753 -0.0053 -4.3 -1.0999999999999996 11.17 5253.0 smORF_v4|nuORFdb_human EIF1B_3:40353577-40353705:+ EIF1B_3:40353577-40353705:+|ENST00000232905.3_1_3:40353564-40353702:+ EIF1B dORF 3:40353577-40353705:+ 3 EIF1B 3' dORF 3' dORF GN=EIF1B E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep1_Slot1-04_1_3496 40mio-rep2 E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep2_Slot2-5_1_3520.4966.1615.3 3 1 1 8.19 8.19 8.19 59.2 8 2 3 6450.0 Hydrogen 33 (L) 10 VTHKKIAVRW (-) 10 V/T/H/K|K|I|A/V R\W 80.0 8 4 7 3 0 unbiased 17.162951 0.740571 413.253 1237.753 -0.0083 -6.7 -1.7999999999999998 11.17 5253.0 smORF_v4|nuORFdb_human EIF1B_3:40353577-40353705:+ EIF1B_3:40353577-40353705:+|ENST00000232905.3_1_3:40353564-40353702:+ EIF1B dORF 3:40353577-40353705:+ 3 EIF1B 3' dORF 3' dORF GN=EIF1B E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep2_Slot2-5_1_3520 40mio-rep3 E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep3_Slot2-5_1_3521.5006.1622.3 3 1 1 9.53 1.5 9.53 8.04 63.2 8 2 3 5930.0 Hydrogen 33 (L) 10 VTHKKIAVRW (-) 10 TKVHIKALQTV V/T/H\K|K/I|A/V R\W 80.0 8 4 6 2 0 unbiased 17.298582 0.736542 413.2534 1237.753 -0.0083 -6.7 -2.1000000000000005 11.17 5253.0 smORF_v4|nuORFdb_human EIF1B_3:40353577-40353705:+ EIF1B_3:40353577-40353705:+|ENST00000232905.3_1_3:40353564-40353702:+ EIF1B dORF 3:40353577-40353705:+ 3 EIF1B 3' dORF 3' dORF GN=EIF1B E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep3_Slot2-5_1_3521 40mio-rep4 E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep4_Slot2-5_1_3522.5020.1608.3 3 1 1 12.92 1.48 9.57 11.44 75.1 9 2 3 12000.0 Hydrogen 33 (L) 10 VTHKKIAVRW (-) 10 GRFIGVKFKSV V/T/H/K/K|I|A/V|R\W 100.0 10 4 8 3 0 y-ion bias 17.344791 0.745069 413.2527 1237.753 -0.0083 -6.7 -2.0 11.17 5253.0 smORF_v4|nuORFdb_human EIF1B_3:40353577-40353705:+ EIF1B_3:40353577-40353705:+|ENST00000232905.3_1_3:40353564-40353702:+ EIF1B dORF 3:40353577-40353705:+ 3 EIF1B 3' dORF 3' dORF GN=EIF1B E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep4_Slot2-5_1_3522 40mio-rep1 E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep1_Slot1-04_1_3496.11416.1480.2 2 1 1 9.01 6.42 3.13 2.59 63.2 5 0 0 6510.0 M25m Hydrogen 22 (P) 9 VTHmGGLNW (K) 9 QVmHLSGGW V T H/m G\G\L|N\W 44.4 4 4 2 1 0 b-ion bias m:Oxidized methionine 39.435467 0.826094 515.7397 1014.483 15.9902 -4.6 -1.3999999999999995 6.71 7928.9 nuORFdb_human ENST00000537956.1_1_12:133563399-133582688:+ ENST00000537956.1_1_12:133563399-133582688:+ ZNF26 ncRNA Processed Transcript lncRNA GN=ZNF26 ncRNA Processed Transcript E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep1_Slot1-04_1_3496 20mio-rep1 E_20221201_NO30_400nL_HLAc1_2e7_directIP_titration_rep1_Slot2-4_1_3515.19638.1442.2 2 2 1 11.18 3.96 7.21 7.21 71.0 7 0 0 16400.0 Hydrogen 19 (K) 9 VTISHPLEW (N) 9 VTDSLLFTW V/T/I/S/H/P L|E\W 77.8 7 2 6 1 0 y-ion bias 67.819008 0.849804 541.2834 1081.568 -0.0089 -8.3 -2.1000000000000005 5.24 4497.3 nuORFdb_human ENST00000418342.5_1_1:23420759-23435517:- ENST00000418342.5_1_1:23420759-23435517:-|ENST00000314174.5_1_1:23420759-23495288:- LUZP1 5' uORF 5' uORF GN=LUZP1 5' uORF E_20221201_NO30_400nL_HLAc1_2e7_directIP_titration_rep1_Slot2-4_1_3515 20mio-rep3 E_20221201_NO30_400nL_HLAc1_2e7_directIP_titration_rep4_Slot2-4_1_3519.19693.1436.2 2 1 1 9.46 2.36 6.83 7.09 67.0 7 0 0 8740.0 Hydrogen 19 (K) 9 VTISHPLEW (N) 9 VTLLAKKcS V/T/I/S/H/P L|E\W 77.8 7 2 6 1 0 y-ion bias 68.015312 0.853508 541.285 1081.568 -0.0049 -4.6 1.2000000000000002 5.24 4497.3 nuORFdb_human ENST00000418342.5_1_1:23420759-23435517:- ENST00000418342.5_1_1:23420759-23435517:-|ENST00000314174.5_1_1:23420759-23495288:- LUZP1 5' uORF 5' uORF GN=LUZP1 5' uORF E_20221201_NO30_400nL_HLAc1_2e7_directIP_titration_rep4_Slot2-4_1_3519 40mio-rep1 E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep1_Slot1-04_1_3496.19883.1423.2 2 2 1 12.93 4.5 8.74 8.43 81.6 7 0 0 15700.0 Hydrogen 19 (K) 9 VTISHPLEW (N) 9 VTLLAKKcS V/T/I/S/H/P L\E\W 77.8 7 2 5 0 0 y-ion bias 68.685646 0.861702 541.2863 1081.568 -0.0029 -2.7 0.5 5.24 4497.3 nuORFdb_human ENST00000418342.5_1_1:23420759-23435517:- ENST00000418342.5_1_1:23420759-23435517:-|ENST00000314174.5_1_1:23420759-23495288:- LUZP1 5' uORF 5' uORF GN=LUZP1 5' uORF E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep1_Slot1-04_1_3496 40mio-rep2 E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep2_Slot2-5_1_3520.19717.1439.2 2 1 1 12.5 4.55 8.19 7.95 75.9 7 0 0 10700.0 Hydrogen 19 (K) 9 VTISHPLEW (N) 9 VTISHTIHSS V/T/I/S|H/P L|E\W 77.8 7 3 6 2 0 y-ion bias 68.113029 0.852101 541.2854 1081.568 -0.0049 -4.6 0.3000000000000007 5.24 4497.3 nuORFdb_human ENST00000418342.5_1_1:23420759-23435517:- ENST00000418342.5_1_1:23420759-23435517:-|ENST00000314174.5_1_1:23420759-23495288:- LUZP1 5' uORF 5' uORF GN=LUZP1 5' uORF E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep2_Slot2-5_1_3520 5mio-rep1 E_20221201_NO30_400nL_HLAc1_5e6_directIP_titration_rep1_Slot1-37_1_3502.19920.1440.2 2 1 1 9.25 3.08 5.75 6.17 73.6 7 0 0 4510.0 Hydrogen 19 (K) 9 VTISHPLEW (N) 9 SLLEHSLPW V/T/I/S/H/P L\E\W 77.8 7 2 5 0 0 y-ion bias 68.802597 0.851464 541.2838 1081.568 -0.0069 -6.4 0.5999999999999996 5.24 4497.3 nuORFdb_human ENST00000418342.5_1_1:23420759-23435517:- ENST00000418342.5_1_1:23420759-23435517:-|ENST00000314174.5_1_1:23420759-23495288:- LUZP1 5' uORF 5' uORF GN=LUZP1 5' uORF E_20221201_NO30_400nL_HLAc1_5e6_directIP_titration_rep1_Slot1-37_1_3502 10mio-rep1 E_20221201_NO30_400nL_HLAc1_1e7_directIP_titration_rep1_Slot2-3_1_3512.15488.1515.2 2 1 1 9.03 5.53 5.64 3.5 74.9 6 1 1 4360.0 Hydrogen 64 (S) 8 VTITPTRW (F) 8 VTLTAAGMPL V/T/I/T|P/T R\W 75.0 6 2 5 1 0 y-ion bias 53.504692 0.804069 487.2725 973.547 -0.0098 -10.1 -2.3999999999999995 9.72 9626.4 nuORFdb_human ENST00000515100.1_1_4:100805248-100813172:- ENST00000515100.1_1_4:100805248-100813172:-|ENST00000226522.8_1_4:100805248-100808497:- LAMTOR3 ncRNA Processed Transcript lncRNA GN=LAMTOR3 ncRNA Processed Transcript E_20221201_NO30_400nL_HLAc1_1e7_directIP_titration_rep1_Slot2-3_1_3512 10mio-rep2 E_20221201_NO30_400nL_HLAc1_1e7_directIP_titration_rep2_Slot2-3_1_3513.15432.1515.2 2 1 1 9.23 6.44 2.79 2.79 77.5 5 1 1 5590.0 Hydrogen 64 (S) 8 VTITPTRW (F) 8 VTLTAAGMPL V/T/I/T|P T R\W 62.5 5 2 4 1 0 y-ion bias 53.308346 0.804246 487.2738 973.547 -0.0058 -6.0 0.7000000000000002 9.72 9626.4 nuORFdb_human ENST00000515100.1_1_4:100805248-100813172:- ENST00000515100.1_1_4:100805248-100813172:-|ENST00000226522.8_1_4:100805248-100808497:- LAMTOR3 ncRNA Processed Transcript lncRNA GN=LAMTOR3 ncRNA Processed Transcript E_20221201_NO30_400nL_HLAc1_1e7_directIP_titration_rep2_Slot2-3_1_3513 20mio-rep2 E_20221201_NO30_400nL_HLAc1_2e7_directIP_titration_rep3_Slot2-4_1_3518.15448.1514.2 2 1 1 10.21 8.69 3.21 1.53 82.1 5 1 1 8830.0 Hydrogen 64 (S) 8 VTITPTRW (F) 8 VTLTAAGMPL V/T/I|T|P/T R W 62.5 5 2 5 2 0 y-ion bias 53.357609 0.80467 487.2734 973.547 -0.0078 -8.0 -1.7000000000000002 9.72 9626.4 nuORFdb_human ENST00000515100.1_1_4:100805248-100813172:- ENST00000515100.1_1_4:100805248-100813172:-|ENST00000226522.8_1_4:100805248-100808497:- LAMTOR3 ncRNA Processed Transcript lncRNA GN=LAMTOR3 ncRNA Processed Transcript E_20221201_NO30_400nL_HLAc1_2e7_directIP_titration_rep3_Slot2-4_1_3518 40mio-rep1 E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep1_Slot1-04_1_3496.15583.1490.2 2 1 1 11.18 9.06 3.68 2.12 83.5 6 1 1 15400.0 Hydrogen 64 (S) 8 VTITPTRW (F) 8 VTLTAAGMPL V/T/I|T|P/T R\W 75.0 6 3 5 2 0 unbiased 53.828178 0.81964 487.2745 973.547 -0.0058 -6.0 -2.8 9.72 9626.4 nuORFdb_human ENST00000515100.1_1_4:100805248-100813172:- ENST00000515100.1_1_4:100805248-100813172:-|ENST00000226522.8_1_4:100805248-100808497:- LAMTOR3 ncRNA Processed Transcript lncRNA GN=LAMTOR3 ncRNA Processed Transcript E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep1_Slot1-04_1_3496 40mio-rep2 E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep2_Slot2-5_1_3520.15301.1507.2 2 1 1 13.57 9.46 4.95 4.11 90.3 6 1 1 12100.0 Hydrogen 64 (S) 8 VTITPTRW (F) 8 VTLTAAGMPL V/T/I|T|P/T R\W 75.0 6 3 5 2 0 unbiased 52.855762 0.809121 487.2745 973.547 -0.0038 -3.9 1.0000000000000004 9.72 9626.4 nuORFdb_human ENST00000515100.1_1_4:100805248-100813172:- ENST00000515100.1_1_4:100805248-100813172:-|ENST00000226522.8_1_4:100805248-100808497:- LAMTOR3 ncRNA Processed Transcript lncRNA GN=LAMTOR3 ncRNA Processed Transcript E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep2_Slot2-5_1_3520 40mio-rep4 E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep4_Slot2-5_1_3522.15303.1508.2 2 1 1 10.88 8.28 5.05 2.61 85.3 5 1 1 16100.0 Hydrogen 64 (S) 8 VTITPTRW (F) 8 VTLTAAGMPL V/T/I/T|P T R\W 62.5 5 2 4 1 0 y-ion bias 52.84948 0.808724 487.2746 973.547 -0.0038 -3.9 0.8000000000000003 9.72 9626.4 nuORFdb_human ENST00000515100.1_1_4:100805248-100813172:- ENST00000515100.1_1_4:100805248-100813172:-|ENST00000226522.8_1_4:100805248-100808497:- LAMTOR3 ncRNA Processed Transcript lncRNA GN=LAMTOR3 ncRNA Processed Transcript E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep4_Slot2-5_1_3522 40mio-rep1 E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep1_Slot1-04_1_3496.24140.1404.2 2 1 1 9.15 7.99 -2.24 1.16 75.1 5 0 0 9090.0 Hydrogen 23 (L) 9 VTLLLQCLV (S) 9 SIILLTAnGR V/T/L|L|L|Q C L V 55.6 5 3 5 3 0 dual b/y bias Carbamidomethylation C:Carbamidomethylation, 83.39254 0.874096 529.8144 1058.628 -0.0071 -6.7 -3.5 5.49 3769.4 nuORFdb_human ENST00000343776.9_1_1:227751263-227751362:+ ENST00000343776.9_1_1:227751263-227751362:+ ZNF678 5' uORF 5' uORF GN=ZNF678 5' uORF E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep1_Slot1-04_1_3496 10mio-rep3 E_20221201_NO30_400nL_HLAc1_1e7_directIP_titration_rep3_Slot2-3_1_3514.23365.1492.2 2 2 1 7.37 3.07 7.37 57.5 6 0 0 3170.0 Hydrogen 4 (A) 9 VTLPVQQAW (I) 9 V T/L|P V|Q|Q\A\W 55.6 5 5 4 3 0 dual b/y bias 80.709114 0.818282 521.286 1041.573 -0.008 -7.7 -1.4000000000000004 5.49 5372.2 smORF_v4|nuORFdb_human FAM214B_9:35108591-35108346:- FAM214B_9:35108591-35108346:-|ENST00000378566.5_1_9:35108314-35108714:-|ENST00000605104.1_1_9:35108540-35108714:- FAM214B uORF 9:35108591-35108346:- 9 FAM214B 5' uORF 5' uORF GN=FAM214B E_20221201_NO30_400nL_HLAc1_1e7_directIP_titration_rep3_Slot2-3_1_3514 20mio-rep1 E_20221201_NO30_400nL_HLAc1_2e7_directIP_titration_rep1_Slot2-4_1_3515.23340.1493.2 2 1 1 10.59 4.51 2.8 6.07 72.5 6 0 0 5090.0 Hydrogen 4 (A) 9 VTLPVQQAW (I) 9 LLSQPGAVADA V T/L|P V|Q/Q|A\W 55.6 5 4 5 3 0 dual b/y bias 80.599777 0.817886 521.2862 1041.573 -0.008 -7.7 -1.5 5.49 5372.2 smORF_v4|nuORFdb_human FAM214B_9:35108591-35108346:- FAM214B_9:35108591-35108346:-|ENST00000378566.5_1_9:35108314-35108714:-|ENST00000605104.1_1_9:35108540-35108714:- FAM214B uORF 9:35108591-35108346:- 9 FAM214B 5' uORF 5' uORF GN=FAM214B E_20221201_NO30_400nL_HLAc1_2e7_directIP_titration_rep1_Slot2-4_1_3515 20mio-rep2 E_20221201_NO30_400nL_HLAc1_2e7_directIP_titration_rep3_Slot2-4_1_3518.23387.1504.2 2 1 1 8.65 3.51 3.81 5.15 69.0 5 0 0 4490.0 Hydrogen 4 (A) 9 VTLPVQQAW (I) 9 LAVVPASQAW V T L/P V/Q|Q|A\W 44.4 4 3 4 2 0 unbiased 80.778893 0.810724 521.2861 1041.573 -0.008 -7.7 -1.4000000000000004 5.49 5372.2 smORF_v4|nuORFdb_human FAM214B_9:35108591-35108346:- FAM214B_9:35108591-35108346:-|ENST00000378566.5_1_9:35108314-35108714:-|ENST00000605104.1_1_9:35108540-35108714:- FAM214B uORF 9:35108591-35108346:- 9 FAM214B 5' uORF 5' uORF GN=FAM214B E_20221201_NO30_400nL_HLAc1_2e7_directIP_titration_rep3_Slot2-4_1_3518 40mio-rep1 E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep1_Slot1-04_1_3496.23578.1472.2 2 3 1 13.77 5.4 5.7 8.37 80.9 7 0 0 19000.0 Hydrogen 4 (A) 9 VTLPVQQAW (I) 9 VTLPGAGAGIW V T/L/P\V|Q|Q\A\W 77.8 7 5 4 2 0 unbiased 81.451729 0.831395 521.2869 1041.573 -0.006 -5.8 -2.5999999999999996 5.49 5372.2 smORF_v4|nuORFdb_human FAM214B_9:35108591-35108346:- FAM214B_9:35108591-35108346:-|ENST00000378566.5_1_9:35108314-35108714:-|ENST00000605104.1_1_9:35108540-35108714:- FAM214B uORF 9:35108591-35108346:- 9 FAM214B 5' uORF 5' uORF GN=FAM214B E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep1_Slot1-04_1_3496 40mio-rep2 E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep2_Slot2-5_1_3520.23351.1497.2 2 3 1 15.32 5.11 4.23 10.21 82.9 7 0 0 16000.0 Hydrogen 4 (A) 9 VTLPVQQAW (I) 9 LSLPLPTDW V T/L|P\V|Q|Q|A\W 77.8 7 6 5 4 0 dual b/y bias 80.666344 0.815564 521.288 1041.573 -0.004 -3.9 1.0000000000000004 5.49 5372.2 smORF_v4|nuORFdb_human FAM214B_9:35108591-35108346:- FAM214B_9:35108591-35108346:-|ENST00000378566.5_1_9:35108314-35108714:-|ENST00000605104.1_1_9:35108540-35108714:- FAM214B uORF 9:35108591-35108346:- 9 FAM214B 5' uORF 5' uORF GN=FAM214B E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep2_Slot2-5_1_3520 40mio-rep3 E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep3_Slot2-5_1_3521.23357.1479.2 2 3 1 10.44 4.49 3.41 5.95 73.8 5 0 0 13900.0 Hydrogen 4 (A) 9 VTLPVQQAW (I) 9 LLSQPGAVADA V T L/P V|Q/Q|A\W 44.4 4 3 4 2 0 unbiased 80.685349 0.826466 521.2874 1041.573 -0.006 -5.8 -1.2000000000000002 5.49 5372.2 smORF_v4|nuORFdb_human FAM214B_9:35108591-35108346:- FAM214B_9:35108591-35108346:-|ENST00000378566.5_1_9:35108314-35108714:-|ENST00000605104.1_1_9:35108540-35108714:- FAM214B uORF 9:35108591-35108346:- 9 FAM214B 5' uORF 5' uORF GN=FAM214B E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep3_Slot2-5_1_3521 40mio-rep4 E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep4_Slot2-5_1_3522.23371.1531.2 2 3 1 13.78 5.13 6.96 8.66 82.7 7 0 0 18000.0 Hydrogen 4 (A) 9 VTLPVQQAW (I) 9 VSLPLQAQW V T/L/P\V|Q|Q|A\W 77.8 7 5 5 3 0 dual b/y bias 80.721687 0.793684 521.2878 1041.573 -0.004 -3.9 0.8000000000000003 5.49 5372.2 smORF_v4|nuORFdb_human FAM214B_9:35108591-35108346:- FAM214B_9:35108591-35108346:-|ENST00000378566.5_1_9:35108314-35108714:-|ENST00000605104.1_1_9:35108540-35108714:- FAM214B uORF 9:35108591-35108346:- 9 FAM214B 5' uORF 5' uORF GN=FAM214B E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep4_Slot2-5_1_3522 20mio-rep1 E_20221201_NO30_400nL_HLAc1_2e7_directIP_titration_rep1_Slot2-4_1_3515.18002.1495.2 2 1 1 11.35 9.02 2.14 2.33 87.1 5 0 0 7220.0 Hydrogen 35 (F) 8 VTQEDLIY (S) 8 SLAEGnLLY V T/Q E/D\L|I\Y 50.0 4 3 3 1 0 unbiased 62.171745 0.816838 490.7461 980.494 -0.0088 -9.0 -2.8 3.67 5326.2 nuORFdb_human ENST00000476195.5_1_1:78050477-78050609:- ENST00000476195.5_1_1:78050477-78050609:-|ENST00000474746.1_1_1:78050477-78050625:- ZZZ3 ncRNA Processed Transcript lncRNA GN=ZZZ3 ncRNA Processed Transcript E_20221201_NO30_400nL_HLAc1_2e7_directIP_titration_rep1_Slot2-4_1_3515 40mio-rep1 E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep1_Slot1-04_1_3496.18541.543.1 1 2 1 8.31 5.84 5.57 2.47 64.5 6 0 0 8300.0 Hydrogen 206 (S) 8 VTQVGVQW (H) 8 VTQAALQW V T/Q|V|G\V|Q\W 75.0 6 5 4 3 0 dual b/y bias 64.049637 1.400153 916.4823 916.489 -0.0067 -7.3 -4.1 5.49 26880.5 nuORFdb_human ENST00000465553.5_1_X:154508114-154563754:- ENST00000465553.5_1_X:154508114-154563754:- CLIC2 5' Overlap uORF 5' Overlap uORF GN=CLIC2 5' Overlap uORF E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep1_Slot1-04_1_3496 10mio-rep2 E_20221201_NO30_400nL_HLAc1_1e7_directIP_titration_rep2_Slot2-3_1_3513.18371.1452.2 2 1 1 9.74 7.44 1.65 2.3 86.7 6 1 1 3430.0 Hydrogen 8 (S) 8 VTRSSFLW (C) 8 ISnSKLFW V/T/R\S S/F|L\W 75.0 6 3 4 1 0 unbiased 63.45789 0.843543 498.2671 995.531 -0.0042 -4.2 2.5 9.72 4827.7 nuORFdb_human ENST00000372455.4_1_1:43852626-43853250:- ENST00000372455.4_1_1:43852626-43853250:- MED8 5' Overlap uORF 5' Overlap uORF GN=MED8 5' Overlap uORF E_20221201_NO30_400nL_HLAc1_1e7_directIP_titration_rep2_Slot2-3_1_3513 10mio-rep3 E_20221201_NO30_400nL_HLAc1_1e7_directIP_titration_rep3_Slot2-3_1_3514.18320.1462.2 2 1 1 11.79 8.72 2.55 3.07 89.7 6 1 1 5500.0 Hydrogen 8 (S) 8 VTRSSFLW (C) 8 VPSGLSHLDA V/T/R/S S\F\L\W 75.0 6 3 3 0 0 unbiased 63.283707 0.837746 498.2674 995.531 -0.0042 -4.2 2.0999999999999996 9.72 4827.7 nuORFdb_human ENST00000372455.4_1_1:43852626-43853250:- ENST00000372455.4_1_1:43852626-43853250:- MED8 5' Overlap uORF 5' Overlap uORF GN=MED8 5' Overlap uORF E_20221201_NO30_400nL_HLAc1_1e7_directIP_titration_rep3_Slot2-3_1_3514 20mio-rep1 E_20221201_NO30_400nL_HLAc1_2e7_directIP_titration_rep1_Slot2-4_1_3515.18327.1452.2 2 1 1 10.92 8.35 1.04 2.57 90.8 6 1 1 6220.0 Hydrogen 8 (S) 8 VTRSSFLW (C) 8 VGTSVFSLW V/T/R\S S\F|L|W 75.0 6 4 4 2 0 unbiased 63.29356 0.843979 498.2677 995.531 -0.0022 -2.2 4.0 9.72 4827.7 nuORFdb_human ENST00000372455.4_1_1:43852626-43853250:- ENST00000372455.4_1_1:43852626-43853250:- MED8 5' Overlap uORF 5' Overlap uORF GN=MED8 5' Overlap uORF E_20221201_NO30_400nL_HLAc1_2e7_directIP_titration_rep1_Slot2-4_1_3515 20mio-rep3 E_20221201_NO30_400nL_HLAc1_2e7_directIP_titration_rep4_Slot2-4_1_3519.18368.1459.2 2 1 1 10.23 7.69 0.96 2.54 87.1 6 1 1 5680.0 Hydrogen 8 (S) 8 VTRSSFLW (C) 8 LSSRSFLW V/T/R\S S\F|L\W 75.0 6 4 3 1 0 unbiased 63.439896 0.839289 498.2668 995.531 -0.0042 -4.2 1.5999999999999996 9.72 4827.7 nuORFdb_human ENST00000372455.4_1_1:43852626-43853250:- ENST00000372455.4_1_1:43852626-43853250:- MED8 5' Overlap uORF 5' Overlap uORF GN=MED8 5' Overlap uORF E_20221201_NO30_400nL_HLAc1_2e7_directIP_titration_rep4_Slot2-4_1_3519 40mio-rep1 E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep1_Slot1-04_1_3496.18570.1437.2 2 2 1 14.98 10.26 4.55 4.72 94.1 6 1 1 39000.0 Hydrogen 8 (S) 8 VTRSSFLW (C) 8 LSSRSFLW V/T/R|S\S F|L|W 75.0 6 4 5 3 0 dual b/y bias 64.149773 0.853391 498.2672 995.531 -0.0042 -4.2 -1.0 9.72 4827.7 nuORFdb_human ENST00000372455.4_1_1:43852626-43853250:- ENST00000372455.4_1_1:43852626-43853250:- MED8 5' Overlap uORF 5' Overlap uORF GN=MED8 5' Overlap uORF E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep1_Slot1-04_1_3496 40mio-rep2 E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep2_Slot2-5_1_3520.18275.1454.2 2 2 1 10.02 7.94 3.1 2.08 80.7 6 1 1 17800.0 Hydrogen 8 (S) 8 VTRSSFLW (C) 8 LSSRSFLW V T/R\S/S\F|L\W 75.0 6 4 3 1 0 unbiased 63.132126 0.842575 498.2665 995.531 -0.0062 -6.2 -1.2999999999999998 9.72 4827.7 nuORFdb_human ENST00000372455.4_1_1:43852626-43853250:- ENST00000372455.4_1_1:43852626-43853250:- MED8 5' Overlap uORF 5' Overlap uORF GN=MED8 5' Overlap uORF E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep2_Slot2-5_1_3520 40mio-rep3 E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep3_Slot2-5_1_3521.18250.1450.2 2 2 1 11.45 8.66 1.55 2.8 93.0 6 1 1 15200.0 Hydrogen 8 (S) 8 VTRSSFLW (C) 8 VAFVNLFW V/T/R\S S\F|L\W 75.0 6 4 3 1 0 unbiased 63.044044 0.844997 498.2675 995.531 -0.0042 -4.2 0.39999999999999947 9.72 4827.7 nuORFdb_human ENST00000372455.4_1_1:43852626-43853250:- ENST00000372455.4_1_1:43852626-43853250:- MED8 5' Overlap uORF 5' Overlap uORF GN=MED8 5' Overlap uORF E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep3_Slot2-5_1_3521 40mio-rep4 E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep4_Slot2-5_1_3522.18247.1448.2 2 2 1 14.42 11.33 3.06 3.09 96.0 6 1 1 14200.0 Hydrogen 8 (S) 8 VTRSSFLW (C) 8 LSSRSFLW V/T/R\S S|F|L\W 75.0 6 4 4 2 0 unbiased 63.016056 0.845939 498.2665 995.531 -0.0042 -4.2 0.5 9.72 4827.7 nuORFdb_human ENST00000372455.4_1_1:43852626-43853250:- ENST00000372455.4_1_1:43852626-43853250:- MED8 5' Overlap uORF 5' Overlap uORF GN=MED8 5' Overlap uORF E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep4_Slot2-5_1_3522 40mio-rep1 E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep1_Slot1-04_1_3496.10041.399.1 1 1 1 8.06 6.11 1.94 1.94 64.7 5 1 1 3070.0 Acetyl 1 (-) 8 VTTKDPDI (I) 8 LSGELSEVP V T/T|K|D|P D\I 50.0 4 4 4 3 0 dual b/y bias 34.687809 1.485932 930.471 930.478 -0.0069 -7.4 -4.2 4.21 3404.0 nuORFdb_human ENST00000405938.5_1_22:26906583-26907086:- ENST00000405938.5_1_22:26906583-26907086:- TFIP11 5' uORF 5' uORF GN=TFIP11 5' uORF E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep1_Slot1-04_1_3496 40mio-rep1 E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep1_Slot1-04_1_3496.15723.1340.2 2 1 1 14.08 11.41 4.94 2.67 89.9 7 1 1 23600.0 Hydrogen 3 (A) 9 VTTRHVLLW (H) 9 VRTSHLLLW V/T/T R\H\V|L|L\W 77.8 7 5 4 2 0 unbiased 54.311573 0.913584 562.8309 1124.658 -0.0028 -2.5 0.7000000000000002 9.73 2529.8 nuORFdb_human ENST00000416221.5_1_1:231658357-231663123:- ENST00000416221.5_1_1:231658357-231663123:- RP11-295G20.2 lincRNA lncRNA GN=RP11-295G20.2 lincRNA E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep1_Slot1-04_1_3496 5mio-rep1 E_20221201_NO30_400nL_HLAc1_5e6_directIP_titration_rep1_Slot1-37_1_3502.15907.1374.2 2 1 1 7.5 5.71 2.94 1.79 61.7 6 1 1 2060.0 Hydrogen 3 (A) 9 VTTRHVLLW (H) 9 VRTSHLLLW V/T/T R H\V\L|L\W 66.7 6 4 3 1 0 unbiased 54.948166 0.892934 562.8302 1124.658 -0.0048 -4.2 2.8 9.73 2529.8 nuORFdb_human ENST00000416221.5_1_1:231658357-231663123:- ENST00000416221.5_1_1:231658357-231663123:- RP11-295G20.2 lincRNA lncRNA GN=RP11-295G20.2 lincRNA E_20221201_NO30_400nL_HLAc1_5e6_directIP_titration_rep1_Slot1-37_1_3502 40mio-rep1 E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep1_Slot1-04_1_3496.15808.546.1 1 2 1 15.3 2.6 7.17 12.69 86.8 9 0 0 6140.0 Hydrogen 159 (F) 10 VTVGAGSEGW (V) 10 GISLGNSEAD V/T/V/G/A|G\S|E\G\W 100.0 10 5 6 2 0 unbiased 54.605869 1.398354 962.4533 962.458 -0.0048 -5.0 -1.7999999999999998 4.0 26815.5 nuORFdb_human ENST00000546624.2_1_12:88177607-88178345:- ENST00000546624.2_1_12:88177607-88178345:- MKRN9P Pseudogene Pseudogene GN=MKRN9P Pseudogene E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep1_Slot1-04_1_3496 40mio-rep2 E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep2_Slot2-5_1_3520.15517.555.1 1 1 1 7.3 7.3 7.3 55.5 7 0 0 3600.0 Hydrogen 159 (F) 10 VTVGAGSEGW (V) 10 V/T V/G/A/G S\E\G\W 60.0 6 3 4 0 0 unbiased 53.60223 1.393162 962.4526 962.458 -0.0048 -5.0 -0.09999999999999964 4.0 26815.5 nuORFdb_human ENST00000546624.2_1_12:88177607-88178345:- ENST00000546624.2_1_12:88177607-88178345:- MKRN9P Pseudogene Pseudogene GN=MKRN9P Pseudogene E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep2_Slot2-5_1_3520 40mio-rep3 E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep3_Slot2-5_1_3521.15521.560.1 1 1 1 10.13 6.79 10.13 73.9 8 0 0 3840.0 Hydrogen 159 (F) 10 VTVGAGSEGW (V) 10 V T/V/G/A/G/S-E\G\W 80.0 8 2 5 0 1 y-ion bias 53.616505 1.390433 962.4527 962.458 -0.0048 -5.0 -0.40000000000000036 4.0 26815.5 nuORFdb_human ENST00000546624.2_1_12:88177607-88178345:- ENST00000546624.2_1_12:88177607-88178345:- MKRN9P Pseudogene Pseudogene GN=MKRN9P Pseudogene E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep3_Slot2-5_1_3521 40mio-rep4 E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep4_Slot2-5_1_3522.15493.562.1 1 2 1 12.6 5.34 12.6 78.3 8 0 0 7080.0 Hydrogen 159 (F) 10 VTVGAGSEGW (V) 10 V T/V/G/A|G/S|E\G\W 80.0 8 4 6 2 0 unbiased 53.505348 1.389104 962.4555 962.458 -0.0018 -1.9 2.8000000000000003 4.0 26815.5 nuORFdb_human ENST00000546624.2_1_12:88177607-88178345:- ENST00000546624.2_1_12:88177607-88178345:- MKRN9P Pseudogene Pseudogene GN=MKRN9P Pseudogene E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep4_Slot2-5_1_3522 10mio-rep1 E_20221201_NO30_400nL_HLAc1_1e7_directIP_titration_rep1_Slot2-3_1_3512.14712.557.1 1 2 1 11.97 3.99 8.46 7.98 81.8 7 0 0 8840.0 Hydrogen 7 (F) 9 VTVTSGESW (K) 9 VVTTSAnSAn V T/V/T|S\G\E|S\W 77.8 7 5 4 2 0 unbiased 50.822681 1.391874 965.4495 965.457 -0.0085 -8.8 -1.1000000000000005 4.0 4973.8 nuORFdb_human ENST00000542165.1_1_11:94704505-94704637:- ENST00000542165.1_1_11:94704505-94704637:- CWC15 ncRNA Retained Intron lncRNA GN=CWC15 ncRNA Retained Intron E_20221201_NO30_400nL_HLAc1_1e7_directIP_titration_rep1_Slot2-3_1_3512 10mio-rep2 E_20221201_NO30_400nL_HLAc1_1e7_directIP_titration_rep2_Slot2-3_1_3513.14658.557.1 1 1 1 13.44 5.76 8.04 7.67 80.5 7 0 0 3850.0 Hydrogen 7 (F) 9 VTVTSGESW (K) 9 VVTTSAnSAn V T/V/T|S|G\E\S\W 77.8 7 5 4 2 0 unbiased 50.636555 1.391704 965.4522 965.457 -0.0055 -5.6 1.1000000000000005 4.0 4973.8 nuORFdb_human ENST00000542165.1_1_11:94704505-94704637:- ENST00000542165.1_1_11:94704505-94704637:- CWC15 ncRNA Retained Intron lncRNA GN=CWC15 ncRNA Retained Intron E_20221201_NO30_400nL_HLAc1_1e7_directIP_titration_rep2_Slot2-3_1_3513 10mio-rep3 E_20221201_NO30_400nL_HLAc1_1e7_directIP_titration_rep3_Slot2-3_1_3514.14619.559.1 1 1 1 13.03 5.71 8.42 7.32 85.5 7 0 0 2500.0 Hydrogen 7 (F) 9 VTVTSGESW (K) 9 ASnSITVSW V T/V/T|S\G\E|S\W 77.8 7 5 4 2 0 unbiased 50.504166 1.390929 965.4526 965.457 -0.0045 -4.6 1.7000000000000002 4.0 4973.8 nuORFdb_human ENST00000542165.1_1_11:94704505-94704637:- ENST00000542165.1_1_11:94704505-94704637:- CWC15 ncRNA Retained Intron lncRNA GN=CWC15 ncRNA Retained Intron E_20221201_NO30_400nL_HLAc1_1e7_directIP_titration_rep3_Slot2-3_1_3514 20mio-rep1 E_20221201_NO30_400nL_HLAc1_2e7_directIP_titration_rep1_Slot2-4_1_3515.14593.556.1 1 4 1 13.39 6.23 8.08 7.16 85.2 7 0 0 25500.0 Hydrogen 7 (F) 9 VTVTSGESW (K) 9 VVTTSAnSAn V T/V/T|S|G\E\S\W 77.8 7 5 4 2 0 unbiased 50.401131 1.392731 965.4509 965.457 -0.0065 -6.7 -0.5 4.0 4973.8 nuORFdb_human ENST00000542165.1_1_11:94704505-94704637:- ENST00000542165.1_1_11:94704505-94704637:- CWC15 ncRNA Retained Intron lncRNA GN=CWC15 ncRNA Retained Intron E_20221201_NO30_400nL_HLAc1_2e7_directIP_titration_rep1_Slot2-4_1_3515 20mio-rep2 E_20221201_NO30_400nL_HLAc1_2e7_directIP_titration_rep3_Slot2-4_1_3518.14701.544.1 1 2 1 13.31 6.78 7.42 6.53 86.8 7 0 0 9340.0 Hydrogen 7 (F) 9 VTVTSGESW (K) 9 VVTTSAnSAn V T/V/T|S|G\E\S\W 77.8 7 5 4 2 0 unbiased 50.778786 1.399459 965.4505 965.457 -0.0075 -7.7 -1.4000000000000004 4.0 4973.8 nuORFdb_human ENST00000542165.1_1_11:94704505-94704637:- ENST00000542165.1_1_11:94704505-94704637:- CWC15 ncRNA Retained Intron lncRNA GN=CWC15 ncRNA Retained Intron E_20221201_NO30_400nL_HLAc1_2e7_directIP_titration_rep3_Slot2-4_1_3518 20mio-rep3 E_20221201_NO30_400nL_HLAc1_2e7_directIP_titration_rep4_Slot2-4_1_3519.14624.546.1 1 2 1 11.43 3.22 9.28 8.2 78.7 7 0 0 9390.0 Hydrogen 7 (F) 9 VTVTSGESW (K) 9 ASnSITVSW V T/V/T/S\G\E|S\W 77.8 7 4 4 1 0 unbiased 50.512207 1.398464 965.4515 965.457 -0.0065 -6.7 -0.9000000000000004 4.0 4973.8 nuORFdb_human ENST00000542165.1_1_11:94704505-94704637:- ENST00000542165.1_1_11:94704505-94704637:- CWC15 ncRNA Retained Intron lncRNA GN=CWC15 ncRNA Retained Intron E_20221201_NO30_400nL_HLAc1_2e7_directIP_titration_rep4_Slot2-4_1_3519 40mio-rep1 E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep1_Slot1-04_1_3496.14907.530.1 1 4 1 14.75 6.54 8.18 8.2 87.8 7 0 0 83800.0 Hydrogen 7 (F) 9 VTVTSGESW (K) 9 ASnSITVSW V T/V/T|S|G\E|S\W 77.8 7 5 5 3 0 dual b/y bias 51.493324 1.408153 965.4549 965.457 -0.0025 -2.5 0.7000000000000002 4.0 4973.8 nuORFdb_human ENST00000542165.1_1_11:94704505-94704637:- ENST00000542165.1_1_11:94704505-94704637:- CWC15 ncRNA Retained Intron lncRNA GN=CWC15 ncRNA Retained Intron E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep1_Slot1-04_1_3496 40mio-rep2 E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep2_Slot2-5_1_3520.14586.517.1 1 4 1 14.07 4.96 8.01 9.1 83.7 7 0 0 58600.0 Hydrogen 7 (F) 9 VTVTSGESW (K) 9 ASnSITVSW V T/V/T|S|G\E|S\W 77.8 7 5 5 3 0 dual b/y bias 50.38628 1.415972 965.4536 965.457 -0.0035 -3.6 1.3000000000000003 4.0 4973.8 nuORFdb_human ENST00000542165.1_1_11:94704505-94704637:- ENST00000542165.1_1_11:94704505-94704637:- CWC15 ncRNA Retained Intron lncRNA GN=CWC15 ncRNA Retained Intron E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep2_Slot2-5_1_3520 40mio-rep3 E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep3_Slot2-5_1_3521.14569.538.1 1 3 1 13.48 3.52 6.91 9.96 81.4 7 0 0 48300.0 Hydrogen 7 (F) 9 VTVTSGESW (K) 9 ASnSITVSW V T/V/T|S|G\E|S\W 77.8 7 5 5 3 0 dual b/y bias 50.329788 1.4032 965.4523 965.457 -0.0055 -5.6 -1.0 4.0 4973.8 nuORFdb_human ENST00000542165.1_1_11:94704505-94704637:- ENST00000542165.1_1_11:94704505-94704637:- CWC15 ncRNA Retained Intron lncRNA GN=CWC15 ncRNA Retained Intron E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep3_Slot2-5_1_3521 40mio-rep4 E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep4_Slot2-5_1_3522.14567.540.1 1 4 1 14.0 5.28 9.18 8.73 87.5 7 0 0 64100.0 Hydrogen 7 (F) 9 VTVTSGESW (K) 9 ASnSITVSW V T/V/T|S\G\E|S\W 77.8 7 5 4 2 0 unbiased 50.308918 1.402094 965.4548 965.457 -0.0025 -2.5 2.2 4.0 4973.8 nuORFdb_human ENST00000542165.1_1_11:94704505-94704637:- ENST00000542165.1_1_11:94704505-94704637:- CWC15 ncRNA Retained Intron lncRNA GN=CWC15 ncRNA Retained Intron E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep4_Slot2-5_1_3522 5mio-rep1 E_20221201_NO30_400nL_HLAc1_5e6_directIP_titration_rep1_Slot1-37_1_3502.14960.547.1 1 1 1 12.53 6.43 7.23 6.1 82.5 8 0 0 2820.0 Hydrogen 7 (F) 9 VTVTSGESW (K) 9 VVTTSAnSAn V/T/V/T|S\G\E\S\W 100.0 9 5 4 1 0 unbiased 51.675228 1.397679 965.4517 965.457 -0.0055 -5.6 1.4000000000000004 4.0 4973.8 nuORFdb_human ENST00000542165.1_1_11:94704505-94704637:- ENST00000542165.1_1_11:94704505-94704637:- CWC15 ncRNA Retained Intron lncRNA GN=CWC15 ncRNA Retained Intron E_20221201_NO30_400nL_HLAc1_5e6_directIP_titration_rep1_Slot1-37_1_3502 5mio-rep2 E_20221201_NO30_400nL_HLAc1_5e6_directIP_titration_rep2_Slot2-2_1_3510.14872.562.1 1 1 1 11.05 4.56 7.08 6.49 79.8 7 0 0 2690.0 Hydrogen 7 (F) 9 VTVTSGESW (K) 9 SLVSTSEGDA V T/V/T/S\G\E\S\W 77.8 7 4 3 0 0 unbiased 51.36657 1.388833 965.4469 965.457 -0.0105 -10.8 -2.1000000000000014 4.0 4973.8 nuORFdb_human ENST00000542165.1_1_11:94704505-94704637:- ENST00000542165.1_1_11:94704505-94704637:- CWC15 ncRNA Retained Intron lncRNA GN=CWC15 ncRNA Retained Intron E_20221201_NO30_400nL_HLAc1_5e6_directIP_titration_rep2_Slot2-2_1_3510 5mio-rep3 E_20221201_NO30_400nL_HLAc1_5e6_directIP_titration_rep3_Slot2-2_1_3511.14893.560.1 1 1 1 9.26 4.99 5.71 4.27 73.1 6 0 0 2480.0 Hydrogen 7 (F) 9 VTVTSGESW (K) 9 VVTTSAnSAn V T/V/T|S\G E\S\W 55.6 5 4 3 1 0 unbiased 51.444473 1.390368 965.4526 965.457 -0.0045 -4.6 3.5999999999999996 4.0 4973.8 nuORFdb_human ENST00000542165.1_1_11:94704505-94704637:- ENST00000542165.1_1_11:94704505-94704637:- CWC15 ncRNA Retained Intron lncRNA GN=CWC15 ncRNA Retained Intron E_20221201_NO30_400nL_HLAc1_5e6_directIP_titration_rep3_Slot2-2_1_3511 20mio-rep3 E_20221201_NO30_400nL_HLAc1_2e7_directIP_titration_rep4_Slot2-4_1_3519.19592.1310.2 2 2 1 9.91 2.85 8.11 7.06 66.3 8 0 1 5060.0 Hydrogen 1 (-) 11 VTWDTAARPEW (-) 11 TVTmALNSEAAAH V/T/W/D|T/A\A/R|P E W 72.7 8 3 7 2 0 y-ion bias 67.666664 0.932755 666.3185 1331.638 -0.0072 -5.4 0.39999999999999947 4.37 1331.4 nuORFdb_human ENST00000409914.6_1_2:130886660-130886696:- ENST00000409914.6_1_2:130886660-130886696:- POTEF 5' uORF 5' uORF GN=POTEF 5' uORF E_20221201_NO30_400nL_HLAc1_2e7_directIP_titration_rep4_Slot2-4_1_3519 40mio-rep1 E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep1_Slot1-04_1_3496.19829.1291.2 2 3 1 14.1 2.61 12.48 11.49 85.4 8 0 1 18600.0 Hydrogen 1 (-) 11 VTWDTAARPEW (-) 11 ATnWISNLPNNS V/T/W/D|T|A\A/R|P E W 72.7 8 4 7 3 0 unbiased 68.499199 0.944129 666.3195 1331.638 -0.0072 -5.4 -2.2 4.37 1331.4 nuORFdb_human ENST00000409914.6_1_2:130886660-130886696:- ENST00000409914.6_1_2:130886660-130886696:- POTEF 5' uORF 5' uORF GN=POTEF 5' uORF E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep1_Slot1-04_1_3496 40mio-rep2 E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep2_Slot2-5_1_3520.19574.1301.2 2 2 1 14.5 3.68 12.13 10.82 80.6 9 0 1 10900.0 Hydrogen 1 (-) 11 VTWDTAARPEW (-) 11 ATnWISNLPNNS V/T/W/D|T|A\A|R|P E\W 81.8 9 6 7 4 0 dual b/y bias 67.6185 0.938146 666.3215 1331.638 -0.0012 -0.9 4.0 4.37 1331.4 nuORFdb_human ENST00000409914.6_1_2:130886660-130886696:- ENST00000409914.6_1_2:130886660-130886696:- POTEF 5' uORF 5' uORF GN=POTEF 5' uORF E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep2_Slot2-5_1_3520 40mio-rep3 E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep3_Slot2-5_1_3521.19601.1308.2 2 2 1 12.16 4.34 10.67 7.82 76.6 8 0 1 8130.0 Hydrogen 1 (-) 11 VTWDTAARPEW (-) 11 ATnWISNLPNNS V/T/W/D|T|A\A/R|P E W 72.7 8 4 7 3 0 unbiased 67.711754 0.934016 666.319 1331.638 -0.0072 -5.4 -0.8000000000000007 4.37 1331.4 nuORFdb_human ENST00000409914.6_1_2:130886660-130886696:- ENST00000409914.6_1_2:130886660-130886696:- POTEF 5' uORF 5' uORF GN=POTEF 5' uORF E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep3_Slot2-5_1_3521 40mio-rep4 E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep4_Slot2-5_1_3522.19598.1305.2 2 3 1 11.56 4.9 9.09 6.66 70.8 8 0 1 11700.0 Hydrogen 1 (-) 11 VTWDTAARPEW (-) 11 ATnWISNLPNNS V/T/W/D|T|A\A|R/P E W 72.7 8 4 7 3 0 unbiased 67.682144 0.936007 666.319 1331.638 -0.0072 -5.4 -0.7000000000000002 4.37 1331.4 nuORFdb_human ENST00000409914.6_1_2:130886660-130886696:- ENST00000409914.6_1_2:130886660-130886696:- POTEF 5' uORF 5' uORF GN=POTEF 5' uORF E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep4_Slot2-5_1_3522 40mio-rep1 E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep1_Slot1-04_1_3496.11456.1372.2 2 5 1 7.41 1.28 4.81 6.13 60.4 7 2 2 41000.0 Hydrogen 84 (R) 9 VVAKAWRSW (-) 9 KVAVnMELVV V/V/A/K|A/W/R\S W 77.8 7 2 6 1 0 y-ion bias 39.573544 0.893644 551.809 1102.616 -0.0049 -4.4 -1.2000000000000002 11.0 10538.0 nuORFdb_human ENST00000576722.5_1_17:1456361-1466031:- ENST00000576722.5_1_17:1456361-1466031:-|ENST00000576761.5_1_17:1456361-1461827:- PITPNA 5' uORF 5' uORF GN=PITPNA 5' uORF E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep1_Slot1-04_1_3496 40mio-rep3 E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep3_Slot2-5_1_3521.11166.1641.3 3 2 1 7.71 5.4 3.32 2.31 81.2 6 2 2 10000.0 Hydrogen 84 (R) 9 VVAKAWRSW (-) 9 VVAEDVVFKP V/V/A/K/A/W/R S W 66.7 6 0 6 0 0 y-ion bias 38.575893 0.72441 368.2087 1102.616 -0.0032 -2.9 1.6999999999999997 11.0 10538.0 nuORFdb_human ENST00000576722.5_1_17:1456361-1466031:- ENST00000576722.5_1_17:1456361-1466031:-|ENST00000576761.5_1_17:1456361-1461827:- PITPNA 5' uORF 5' uORF GN=PITPNA 5' uORF E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep3_Slot2-5_1_3521 40mio-rep4 E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep4_Slot2-5_1_3522.11183.1639.3 3 1 1 9.42 5.86 4.06 3.56 89.2 6 2 2 5580.0 Hydrogen 84 (R) 9 VVAKAWRSW (-) 9 VVAEDVVFKP V/V/A/K/A/W/R S W 66.7 6 0 6 0 0 y-ion bias 38.620361 0.725622 368.2082 1102.616 -0.0062 -5.6 -0.8999999999999995 11.0 10538.0 nuORFdb_human ENST00000576722.5_1_17:1456361-1466031:- ENST00000576722.5_1_17:1456361-1466031:-|ENST00000576761.5_1_17:1456361-1461827:- PITPNA 5' uORF 5' uORF GN=PITPNA 5' uORF E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep4_Slot2-5_1_3522 40mio-rep1 E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep1_Slot1-04_1_3496.21797.1273.2 2 1 1 9.73 1.94 8.99 7.79 68.5 6 1 1 5600.0 Hydrogen 150 (L) 11 VVDVSGKFFEY (T) 11 VVDVnSAGERSR V V/D|V|S|G K F F\E\Y 45.5 5 5 4 3 0 unbiased 75.297714 0.955411 645.3231 1289.641 -0.0025 -1.9 1.3000000000000003 4.37 20398.2 nuORFdb_human ENST00000554295.1_1_12:98850353-98850914:- ENST00000554295.1_1_12:98850353-98850914:- SLC9A7P1 Pseudogene Pseudogene GN=SLC9A7P1 Pseudogene E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep1_Slot1-04_1_3496 SCP E_20221201_NO30_400nL_HLAc1_4e7_PDAC_Slot2-6_1_3524.14274.659.1 1 1 1 11.02 9.51 2.25 1.51 76.4 6 0 0 9030.0 Hydrogen 38 (S) 8 VVGVTLGP (M) 8 VVKEPGL V/V/G V|T|L\G-P 75.0 6 3 4 2 1 unbiased 49.311867 1.330623 741.4488 741.451 -0.0015 -2.0 0.3999999999999999 5.49 61862.3 nuORFdb_human ENST00000394321.9_1_19:7591427-7598678:+ ENST00000394321.9_1_19:7591427-7598678:+ MCOLN1 3' Overlap dORF 3' Overlap dORF GN=MCOLN1 3' Overlap dORF E_20221201_NO30_400nL_HLAc1_4e7_PDAC_Slot2-6_1_3524 10mio-rep1 E_20221201_NO30_400nL_HLAc1_1e7_directIP_titration_rep1_Slot2-3_1_3512.14194.434.1 1 1 1 10.75 2.54 7.73 8.22 72.0 7 0 0 6150.0 Hydrogen 46 (G) 9 VVLDSTQEV (-) 9 VVnLSTENI V V/L/D\S|T/Q\E\V 77.8 7 4 4 1 0 unbiased 49.034359 1.465289 989.5072 989.515 -0.008 -8.1 -0.39999999999999947 3.66 5807.6 nuORFdb_human T309521_6:110863432-110863594:- T309521_6:110863432-110863594:- lincRNA lncRNA GN=nan lincRNA E_20221201_NO30_400nL_HLAc1_1e7_directIP_titration_rep1_Slot2-3_1_3512 10mio-rep2 E_20221201_NO30_400nL_HLAc1_1e7_directIP_titration_rep2_Slot2-3_1_3513.14152.434.1 1 1 1 12.92 5.69 8.58 7.23 85.6 7 0 0 3780.0 Hydrogen 46 (G) 9 VVLDSTQEV (-) 9 VVnLSTENI V V/L/D\S|T|Q\E\V 77.8 7 5 4 2 0 unbiased 48.889614 1.465175 989.5102 989.515 -0.005 -5.0 1.7000000000000002 3.66 5807.6 nuORFdb_human T309521_6:110863432-110863594:- T309521_6:110863432-110863594:- lincRNA lncRNA GN=nan lincRNA E_20221201_NO30_400nL_HLAc1_1e7_directIP_titration_rep2_Slot2-3_1_3513 10mio-rep3 E_20221201_NO30_400nL_HLAc1_1e7_directIP_titration_rep3_Slot2-3_1_3514.14090.433.1 1 1 1 13.18 5.94 9.49 7.24 79.1 7 0 0 2530.0 Hydrogen 46 (G) 9 VVLDSTQEV (-) 9 VVnLSTENI V V/L/D|S\T|Q\E\V 77.8 7 5 4 2 0 unbiased 48.677647 1.465798 989.5077 989.515 -0.007 -7.0 -0.7000000000000002 3.66 5807.6 nuORFdb_human T309521_6:110863432-110863594:- T309521_6:110863432-110863594:- lincRNA lncRNA GN=nan lincRNA E_20221201_NO30_400nL_HLAc1_1e7_directIP_titration_rep3_Slot2-3_1_3514 20mio-rep1 E_20221201_NO30_400nL_HLAc1_2e7_directIP_titration_rep1_Slot2-4_1_3515.14068.435.1 1 1 1 13.91 6.06 9.45 7.85 85.8 7 0 0 2550.0 Hydrogen 46 (G) 9 VVLDSTQEV (-) 9 VVnLSTENI V V/L/D|S|T|Q\E\V 77.8 7 5 5 3 0 dual b/y bias 48.58886 1.464808 989.5069 989.515 -0.008 -8.1 -1.8999999999999995 3.66 5807.6 nuORFdb_human T309521_6:110863432-110863594:- T309521_6:110863432-110863594:- lincRNA lncRNA GN=nan lincRNA E_20221201_NO30_400nL_HLAc1_2e7_directIP_titration_rep1_Slot2-4_1_3515 20mio-rep2 E_20221201_NO30_400nL_HLAc1_2e7_directIP_titration_rep3_Slot2-4_1_3518.14161.421.1 1 1 1 12.17 4.93 9.16 7.24 78.4 7 0 0 6140.0 Hydrogen 46 (G) 9 VVLDSTQEV (-) 9 VVnLSTENI V V/L/D|S|T|Q\E\V 77.8 7 5 5 3 0 dual b/y bias 48.914795 1.472735 989.5041 989.515 -0.011 -11.1 -4.8 3.66 5807.6 nuORFdb_human T309521_6:110863432-110863594:- T309521_6:110863432-110863594:- lincRNA lncRNA GN=nan lincRNA E_20221201_NO30_400nL_HLAc1_2e7_directIP_titration_rep3_Slot2-4_1_3518 20mio-rep3 E_20221201_NO30_400nL_HLAc1_2e7_directIP_titration_rep4_Slot2-4_1_3519.14087.420.1 1 2 1 16.51 8.15 12.23 8.36 92.7 7 0 0 6700.0 Hydrogen 46 (G) 9 VVLDSTQEV (-) 9 VVnLSTENI V V/L/D|S|T|Q\E\V 77.8 7 5 5 3 0 dual b/y bias 48.658237 1.473308 989.5101 989.515 -0.005 -5.0 0.7999999999999998 3.66 5807.6 nuORFdb_human T309521_6:110863432-110863594:- T309521_6:110863432-110863594:- lincRNA lncRNA GN=nan lincRNA E_20221201_NO30_400nL_HLAc1_2e7_directIP_titration_rep4_Slot2-4_1_3519 40mio-rep1 E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep1_Slot1-04_1_3496.14401.404.1 1 2 1 13.65 5.31 10.16 8.33 82.3 7 0 0 42000.0 Hydrogen 46 (G) 9 VVLDSTQEV (-) 9 VVnLSTENI V V/L/D|S|T|Q\E\V 77.8 7 5 5 3 0 dual b/y bias 49.745747 1.483099 989.5117 989.515 -0.003 -3.0 0.20000000000000018 3.66 5807.6 nuORFdb_human T309521_6:110863432-110863594:- T309521_6:110863432-110863594:- lincRNA lncRNA GN=nan lincRNA E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep1_Slot1-04_1_3496 40mio-rep2 E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep2_Slot2-5_1_3520.14050.420.1 1 1 1 11.7 5.99 10.11 5.71 76.0 7 0 0 11000.0 Hydrogen 46 (G) 9 VVLDSTQEV (-) 9 VVnLSTENI V V/L/D|S|T|Q\E\V 77.8 7 5 5 3 0 dual b/y bias 48.534985 1.473464 989.5142 989.515 -0.001 -1.0 3.9000000000000004 3.66 5807.6 nuORFdb_human T309521_6:110863432-110863594:- T309521_6:110863432-110863594:- lincRNA lncRNA GN=nan lincRNA E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep2_Slot2-5_1_3520 40mio-rep3 E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep3_Slot2-5_1_3521.14060.419.1 1 2 1 12.63 4.43 8.67 8.2 80.3 7 0 0 23500.0 Hydrogen 46 (G) 9 VVLDSTQEV (-) 9 VVnLSTENI V V/L/D\S|T/Q\E\V 77.8 7 4 4 1 0 unbiased 48.572456 1.473836 989.5098 989.515 -0.005 -5.0 -0.40000000000000036 3.66 5807.6 nuORFdb_human T309521_6:110863432-110863594:- T309521_6:110863432-110863594:- lincRNA lncRNA GN=nan lincRNA E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep3_Slot2-5_1_3521 40mio-rep4 E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep4_Slot2-5_1_3522.14034.415.1 1 2 1 14.01 7.08 9.03 6.93 85.0 7 0 0 22600.0 Hydrogen 46 (G) 9 VVLDSTQEV (-) 9 VVnLSTENI V V/L/D|S|T\Q\E\V 77.8 7 5 4 2 0 unbiased 48.466118 1.476304 989.5104 989.515 -0.005 -5.0 -0.2999999999999998 3.66 5807.6 nuORFdb_human T309521_6:110863432-110863594:- T309521_6:110863432-110863594:- lincRNA lncRNA GN=nan lincRNA E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep4_Slot2-5_1_3522 Eva-50mio-3fr AL20210827_EV_HLA_PDAC_PANFRO413_2D_50e6_W632_classI_fxn3.38829.38928.2 2 1 1 15.13 6.44 8.66 8.69 76.1 8 1 1 105000000.0 Hydrogen 24 (D) 10 VWMRSPLSTF (-) 10 GQSWVKRDTF V/W|M/R\S\P L\S|T|F 80.0 8 6 5 3 0 unbiased 0.953 90.6 70.75 100.0 0.0 612.3172 1223.624 0.0029 2.4 1.9 9.72 3857.5 nuORFdb_human ENST00000559725.5_1_15:60656692-60682421:- ENST00000559725.5_1_15:60656692-60682421:-|ENST00000558985.5_1_15:60656692-60690187:-|ENST00000558998.5_1_15:60656689-60689459:- ANXA2 3' Overlap dORF 3' Overlap dORF GN=ANXA2 3' Overlap dORF AL20210827_EV_HLA_PDAC_PANFRO413_2D_50e6_W632_classI_fxn3 Exploris AL20221201_MKP_PDAC_01.57645.58394.2 2 1 1 17.13 7.7 8.15 9.43 84.5 8 1 1 2750000000.0 Hydrogen 24 (D) 10 VWMRSPLSTF (-) 10 GQSWVKRDTF V/W|M/R\S\P L\S|T|F 80.0 8 6 5 3 0 unbiased 0.996 100.0 88.1 83.84 0.0 612.3162 1223.624 0.001 0.8 0.0 9.72 3857.5 nuORFdb_human ENST00000559725.5_1_15:60656692-60682421:- ENST00000559725.5_1_15:60656692-60682421:-|ENST00000558985.5_1_15:60656692-60690187:-|ENST00000558998.5_1_15:60656689-60689459:- ANXA2 3' Overlap dORF 3' Overlap dORF GN=ANXA2 3' Overlap dORF AL20221201_MKP_PDAC_01 SCP E_20221201_NO30_400nL_HLAc1_4e7_PDAC_Slot2-6_1_3524.27612.1304.2 2 5 1 17.09 5.85 8.67 11.23 84.2 8 1 1 70600.0 Hydrogen 24 (D) 10 VWMRSPLSTF (-) 10 PSRSELISSTF V/W|M/R|S\P L|S|T\F 80.0 8 6 6 4 0 dual b/y bias 95.395103 0.936357 612.315 1223.624 -0.0014 -1.2 1.2 9.72 3857.5 nuORFdb_human ENST00000559725.5_1_15:60656692-60682421:- ENST00000559725.5_1_15:60656692-60682421:-|ENST00000558985.5_1_15:60656692-60690187:-|ENST00000558998.5_1_15:60656689-60689459:- ANXA2 3' Overlap dORF 3' Overlap dORF GN=ANXA2 3' Overlap dORF E_20221201_NO30_400nL_HLAc1_4e7_PDAC_Slot2-6_1_3524 Exploris_FAIMS VH20221207_MKP_FAIMS2022E_HLAc1_4e7_PDAC.61195.61195.2 2 1 1 15.04 5.09 8.82 9.95 76.9 8 1 1 237000000.0 Hydrogen 24 (D) 10 VWMRSPLSTF (-) 10 PSRSELISSTF V/W|M/R\S\P L\S|T/F 80.0 8 5 5 2 0 unbiased 1.0 100.0 106.0 9.95 -50.0 612.3156 1223.624 -0.0002 -0.2 2.4 9.72 3857.5 nuORFdb_human ENST00000559725.5_1_15:60656692-60682421:- ENST00000559725.5_1_15:60656692-60682421:-|ENST00000558985.5_1_15:60656692-60690187:-|ENST00000558998.5_1_15:60656689-60689459:- ANXA2 3' Overlap dORF 3' Overlap dORF GN=ANXA2 3' Overlap dORF VH20221207_MKP_FAIMS2022E_HLAc1_4e7_PDAC Eva-50mio-3fr AL20210827_EV_HLA_PDAC_PANFRO413_2D_50e6_W632_classI_fxn3.31574.31777.2 2 1 1 12.11 2.29 9.82 9.82 67.5 7 1 1 861000000.0 M26m Hydrogen 24 (D) 10 VWmRSPLSTF (-) 10 SPFTPDKLSTF V W|m/R S\P\L\S|T/F 60.0 6 5 4 2 0 unbiased m:Oxidized methionine 0.995 99.8 58.05 2.73 0.0 620.3131 1223.624 15.9947 -0.2 -0.7 9.72 3857.5 nuORFdb_human ENST00000559725.5_1_15:60656692-60682421:- ENST00000559725.5_1_15:60656692-60682421:-|ENST00000558985.5_1_15:60656692-60690187:-|ENST00000558998.5_1_15:60656689-60689459:- ANXA2 3' Overlap dORF 3' Overlap dORF GN=ANXA2 3' Overlap dORF AL20210827_EV_HLA_PDAC_PANFRO413_2D_50e6_W632_classI_fxn3 Exploris AL20221201_MKP_PDAC_01.42327.43136.2 2 3 1 12.42 3.39 9.04 9.04 65.1 7 1 1 3080000000.0 M26m Hydrogen 24 (D) 10 VWmRSPLSTF (-) 10 EWAKRAGSSTF V W|m/R S\P\L\S|T|F 60.0 6 6 4 3 0 unbiased m:Oxidized methionine 0.993 98.1 64.98 13.14 0.0 620.3139 1223.624 15.9964 1.2 0.3999999999999999 9.72 3857.5 nuORFdb_human ENST00000559725.5_1_15:60656692-60682421:- ENST00000559725.5_1_15:60656692-60682421:-|ENST00000558985.5_1_15:60656692-60690187:-|ENST00000558998.5_1_15:60656689-60689459:- ANXA2 3' Overlap dORF 3' Overlap dORF GN=ANXA2 3' Overlap dORF AL20221201_MKP_PDAC_01 Exploris_FAIMS VH20221207_MKP_FAIMS2022E_HLAc1_4e7_PDAC.49502.50542.2 2 4 1 11.04 1.09 10.23 9.96 60.8 7 1 1 1040000000.0 M26m Hydrogen 24 (D) 10 VWmRSPLSTF (-) 10 PAHPCLLPSTF V W|m/R S\P\L\S|T/F 60.0 6 5 4 2 0 unbiased m:Oxidized methionine 0.996 87.0 88.87 120.0 -50.0 620.3118 1223.624 15.9922 -2.2 0.3999999999999999 9.72 3857.5 nuORFdb_human ENST00000559725.5_1_15:60656692-60682421:- ENST00000559725.5_1_15:60656692-60682421:-|ENST00000558985.5_1_15:60656692-60690187:-|ENST00000558998.5_1_15:60656689-60689459:- ANXA2 3' Overlap dORF 3' Overlap dORF GN=ANXA2 3' Overlap dORF VH20221207_MKP_FAIMS2022E_HLAc1_4e7_PDAC SCP E_20221201_NO30_400nL_HLAc1_4e7_PDAC_Slot2-6_1_3524.17816.233.1 1 2 1 10.61 -0.98 9.36 11.59 73.5 7 0 0 5700.0 M40m M42m Hydrogen 38 (S) 9 VWmSmTTTW (L) 9 TcLVTMTTW V W/m|S/m\T\T\T\W 77.8 7 5 3 1 0 unbiased m:Oxidized methionine 61.549274 1.583787 1174.4883 1142.501 31.9891 -0.6 1.7999999999999998 5.49 6688.5 nuORFdb_human ENST00000263270.10_1_19:47342049-47349305:- ENST00000263270.10_1_19:47342049-47349305:- AP2S1 Out-of-Frame Out-of-Frame GN=AP2S1 Out-of-Frame E_20221201_NO30_400nL_HLAc1_4e7_PDAC_Slot2-6_1_3524 Exploris AL20221201_MKP_PDAC_01.21175.21175.3 3 1 1 11.44 6.18 11.44 70.5 6 1 2 42100000.0 Hydrogen 13 (K) 9 VYCSFTRKL (I) 9 V/Y/C/S/F/T R K-L 66.7 6 0 5 0 1 y-ion bias Carbamidomethylation C:Carbamidomethylation, 0.979 100.0 36.27 19.4 0.0 391.8752 1173.609 0.0027 2.3 1.4999999999999998 9.99 3636.3 nuORFdb_human ENST00000246548.8_1_19:34921541-34922835:+ ENST00000246548.8_1_19:34921541-34922835:+ UBA2 Out-of-Frame Out-of-Frame GN=UBA2 Out-of-Frame AL20221201_MKP_PDAC_01 SCP E_20221201_NO30_400nL_HLAc1_4e7_PDAC_Slot2-6_1_3524.9667.1295.2 2 1 1 15.96 8.46 8.47 7.51 89.7 8 1 2 7690.0 Hydrogen 13 (K) 9 VYCSFTRKL (I) 9 LKKScTLYV V/Y|C|S/F/T/R\K\L 100.0 9 4 6 2 0 unbiased Carbamidomethylation C:Carbamidomethylation, 33.398003 0.941791 587.3088 1173.609 0.0022 1.9 4.3 9.99 3636.3 nuORFdb_human ENST00000246548.8_1_19:34921541-34922835:+ ENST00000246548.8_1_19:34921541-34922835:+ UBA2 Out-of-Frame Out-of-Frame GN=UBA2 Out-of-Frame E_20221201_NO30_400nL_HLAc1_4e7_PDAC_Slot2-6_1_3524 Exploris_FAIMS VH20221207_MKP_FAIMS2022E_HLAc1_4e7_PDAC.27061.27061.3 3 1 1 12.0 7.62 12.0 74.9 6 1 2 10700000.0 Hydrogen 13 (K) 9 VYCSFTRKL (I) 9 V Y/C/S/F/T/R K-L 55.6 5 0 5 0 1 y-ion bias Carbamidomethylation C:Carbamidomethylation, 0.992 86.2 54.35 24.83 -50.0 391.8731 1173.609 -0.0036 -3.1 -0.5 9.99 3636.3 nuORFdb_human ENST00000246548.8_1_19:34921541-34922835:+ ENST00000246548.8_1_19:34921541-34922835:+ UBA2 Out-of-Frame Out-of-Frame GN=UBA2 Out-of-Frame VH20221207_MKP_FAIMS2022E_HLAc1_4e7_PDAC Eva-50mio-3fr AL20210827_EV_HLA_PDAC_PANFRO413_2D_50e6_W632_classI_fxn3.42857.42857.2 2 1 1 9.85 9.85 9.85 62.7 5 0 0 22700000.0 Hydrogen 56 (R) 9 VYFGHWVVM (N) 9 V Y\F G H\W\V|V/M 44.4 4 4 2 1 0 b-ion bias 0.983 66.1 77.68 32.56 0.0 569.2816 1137.555 0.001 0.8 0.30000000000000004 6.71 17668.9 nuORFdb_human ENST00000412609.5_1_19:40170059-40174276:+ ENST00000412609.5_1_19:40170059-40174276:+|ENST00000598164.2_1_19:40172096-40174784:+ LGALS17A Pseudogene Pseudogene GN=LGALS17A Pseudogene AL20210827_EV_HLA_PDAC_PANFRO413_2D_50e6_W632_classI_fxn3 Exploris AL20221201_MKP_PDAC_01.63556.63680.2 2 1 1 14.7 14.7 14.7 84.6 5 0 0 1050000000.0 Hydrogen 56 (R) 9 VYFGHWVVM (N) 9 V Y\F G H\W\V|V/M 44.4 4 4 2 1 0 b-ion bias 0.979 79.9 95.98 1.19 0.0 569.2816 1137.555 0.001 0.9 0.09999999999999998 6.71 17668.9 nuORFdb_human ENST00000412609.5_1_19:40170059-40174276:+ ENST00000412609.5_1_19:40170059-40174276:+|ENST00000598164.2_1_19:40172096-40174784:+ LGALS17A Pseudogene Pseudogene GN=LGALS17A Pseudogene AL20221201_MKP_PDAC_01 SCP E_20221201_NO30_400nL_HLAc1_4e7_PDAC_Slot2-6_1_3524.28096.181.1 1 2 1 12.43 2.41 9.55 10.02 90.4 6 0 0 69100.0 Hydrogen 56 (R) 9 VYFGHWVVM (N) 9 LVMEETSVVM V Y/F G/H|W\V\V\M 55.6 5 4 3 1 0 unbiased 97.066963 1.614424 1137.5548 1137.555 -0.005 -4.4 -2.0000000000000004 6.71 17668.9 nuORFdb_human ENST00000412609.5_1_19:40170059-40174276:+ ENST00000412609.5_1_19:40170059-40174276:+|ENST00000598164.2_1_19:40172096-40174784:+ LGALS17A Pseudogene Pseudogene GN=LGALS17A Pseudogene E_20221201_NO30_400nL_HLAc1_4e7_PDAC_Slot2-6_1_3524 Eva-50mio-3fr AL20210827_EV_HLA_PDAC_PANFRO413_2D_50e6_W632_classI_fxn3.35118.35328.2 2 1 1 16.48 0.78 15.06 15.7 95.1 6 0 0 258000000.0 M64m Hydrogen 56 (R) 9 VYFGHWVVm (N) 9 VYDnLITPAm V Y\F/G H|W\V|V/m 55.6 5 4 4 2 0 unbiased m:Oxidized methionine 0.978 90.2 64.35 32.68 0.0 577.2791 1137.555 15.996 0.9 0.4 6.71 17668.9 nuORFdb_human ENST00000412609.5_1_19:40170059-40174276:+ ENST00000412609.5_1_19:40170059-40174276:+|ENST00000598164.2_1_19:40172096-40174784:+ LGALS17A Pseudogene Pseudogene GN=LGALS17A Pseudogene AL20210827_EV_HLA_PDAC_PANFRO413_2D_50e6_W632_classI_fxn3 Exploris AL20221201_MKP_PDAC_01.46614.47507.2 2 1 1 14.08 12.15 14.08 83.8 6 0 0 716000000.0 M64m Hydrogen 56 (R) 9 VYFGHWVVm (N) 9 V Y\F/G H\W\V|V|m 55.6 5 5 3 2 0 unbiased m:Oxidized methionine 0.998 100.0 72.5 64.61 0.0 577.2799 1137.555 15.9975 2.3 1.4999999999999998 6.71 17668.9 nuORFdb_human ENST00000412609.5_1_19:40170059-40174276:+ ENST00000412609.5_1_19:40170059-40174276:+|ENST00000598164.2_1_19:40172096-40174784:+ LGALS17A Pseudogene Pseudogene GN=LGALS17A Pseudogene AL20221201_MKP_PDAC_01 SCP E_20221201_NO30_400nL_HLAc1_4e7_PDAC_Slot2-6_1_3524.22379.1332.2 2 9 1 13.0 2.23 10.77 10.77 75.4 8 0 0 38800.0 M64m Hydrogen 56 (R) 9 VYFGHWVVm (N) 9 VYPGKITDNm V/Y|F/G/H|W\V|V\m 100.0 9 5 6 3 0 dual b/y bias m:Oxidized methionine 77.31469 0.918786 577.278 1137.555 15.9937 -1.0 1.4 6.71 17668.9 nuORFdb_human ENST00000412609.5_1_19:40170059-40174276:+ ENST00000412609.5_1_19:40170059-40174276:+|ENST00000598164.2_1_19:40172096-40174784:+ LGALS17A Pseudogene Pseudogene GN=LGALS17A Pseudogene E_20221201_NO30_400nL_HLAc1_4e7_PDAC_Slot2-6_1_3524 SCP E_20221201_NO30_400nL_HLAc1_4e7_PDAC_Slot2-6_1_3524.25994.448.1 1 1 1 8.81 4.25 2.8 4.56 75.5 6 0 0 5950.0 Hydrogen 47 (W) 9 VYFGSVPEL (V) 9 EGPGNVVPEL V Y/F\G\S|V|P-E L 55.6 5 4 3 2 1 unbiased 89.801117 1.456945 1010.5188 1010.519 0.0007 0.7 3.0999999999999996 4.0 7764.9 nuORFdb_human ENST00000504622.5_1_5:82352937-82373269:- ENST00000504622.5_1_5:82352937-82373269:-|ENST00000502346.1_1_5:82352934-82360830:- TMEM167A 5' Overlap uORF 5' Overlap uORF GN=TMEM167A 5' Overlap uORF E_20221201_NO30_400nL_HLAc1_4e7_PDAC_Slot2-6_1_3524 SCP E_20221201_NO30_400nL_HLAc1_4e7_PDAC_Slot2-6_1_3524.25884.1220.2 2 3 1 16.73 6.9 8.37 9.83 86.2 7 1 1 19400.0 Hydrogen 50 (R) 11 VYFRFTNTASF (-) 11 GQNVIEVMSAASF V Y|F R F|T\N|T|A|S\F 54.5 6 7 5 5 0 dual b/y bias 89.421303 0.988453 676.8351 1352.663 -0.0006 -0.5 1.9 8.72 7120.8 nuORFdb_human ENST00000614364.1_1_13:53029172-53029352:- ENST00000614364.1_1_13:53029172-53029352:- RP11-245D16.4 lincRNA lncRNA GN=RP11-245D16.4 lincRNA E_20221201_NO30_400nL_HLAc1_4e7_PDAC_Slot2-6_1_3524 Eva-50mio-3fr AL20210827_EV_HLA_PDAC_PANFRO413_2D_50e6_W632_classI_fxn3.28235.28235.3 3 1 1 13.26 2.96 11.67 10.3 88.2 7 1 2 16600000.0 Hydrogen 72 (A) 11 VYFTDFKKSEF (-) 11 FTAPLPASEPHFP V Y|F/T/D/F/K/K S E/F 54.5 6 1 7 1 0 y-ion bias 0.995 100.0 51.75 100.0 0.0 470.9042 1410.694 0.0041 2.9 2.4 6.04 9728.0 nuORFdb_human ENST00000426519.1_1_1:166445243-166446165:- ENST00000426519.1_1_1:166445243-166446165:- LINC01675 lincRNA lncRNA GN=LINC01675 lincRNA AL20210827_EV_HLA_PDAC_PANFRO413_2D_50e6_W632_classI_fxn3 SCP E_20221201_NO30_400nL_HLAc1_4e7_PDAC_Slot2-6_1_3524.17473.1157.2 2 2 1 15.28 5.95 8.7 9.33 88.9 9 1 2 20400.0 Hydrogen 72 (A) 11 VYFTDFKKSEF (-) 11 VYFVTTLGYNFS V/Y|F|T/D|F/K/K S-E\F 81.8 9 4 7 3 1 unbiased 60.364124 1.027824 705.8504 1410.694 -0.0013 -0.9 1.5 6.04 9728.0 nuORFdb_human ENST00000426519.1_1_1:166445243-166446165:- ENST00000426519.1_1_1:166445243-166446165:- LINC01675 lincRNA lncRNA GN=LINC01675 lincRNA E_20221201_NO30_400nL_HLAc1_4e7_PDAC_Slot2-6_1_3524 Eva-50mio-3fr AL20210827_EV_HLA_PDAC_PANFRO413_2D_50e6_W632_classI_fxn2.34534.34534.2 2 2 2 13.83 3.35 9.37 10.48 86.1 8 1 1 12500000.0 Hydrogen 52 (Y) 10 VYFTNELKQF (A) 10 VYIIDYAREF V Y|F/T-N-E/L/K/Q/F 80.0 8 1 6 1 2 y-ion bias 0.976 100.0 63.33 100.0 0.0 644.8322 1288.657 -0.0 -0.0 -0.3 5.97 9035.3 nuORFdb_human ENST00000466777.1_1_3:172236192-172241222:- ENST00000466777.1_1_3:172236192-172241222:- TNFSF10 5' Overlap uORF 5' Overlap uORF GN=TNFSF10 5' Overlap uORF AL20210827_EV_HLA_PDAC_PANFRO413_2D_50e6_W632_classI_fxn2 Eva-50mio-3fr AL20210827_EV_HLA_PDAC_PANFRO413_2D_50e6_W632_classI_fxn3.34189.34301.2 2 1 1 13.46 9.81 5.04 3.66 94.2 7 1 1 634000000.0 Hydrogen 39 (G) 9 VYGFLRNGF (-) 9 VYGFMMLVL V/Y|G|F/L/R-N G/F 77.8 7 2 6 2 1 y-ion bias 0.999 97.7 62.67 12.97 0.0 536.7822 1072.557 -0.0002 -0.2 -0.7 8.72 5094.7 nuORFdb_human ENST00000343327.6_1_9:115030452-115095785:- ENST00000343327.6_1_9:115030452-115095785:- PTBP3 5' Overlap uORF 5' Overlap uORF GN=PTBP3 5' Overlap uORF AL20210827_EV_HLA_PDAC_PANFRO413_2D_50e6_W632_classI_fxn3 Exploris AL20221201_MKP_PDAC_01.47676.48388.2 2 7 1 12.67 9.01 5.46 3.66 87.0 7 1 1 5250000000.0 Hydrogen 39 (G) 9 VYGFLRNGF (-) 9 VYGFMMLVL V/Y|G|F/L/R-N G/F 77.8 7 2 6 2 1 y-ion bias 0.997 88.2 73.15 8.97 0.0 536.7827 1072.557 0.0006 0.6 -0.20000000000000007 8.72 5094.7 nuORFdb_human ENST00000343327.6_1_9:115030452-115095785:- ENST00000343327.6_1_9:115030452-115095785:- PTBP3 5' Overlap uORF 5' Overlap uORF GN=PTBP3 5' Overlap uORF AL20221201_MKP_PDAC_01 SCP E_20221201_NO30_400nL_HLAc1_4e7_PDAC_Slot2-6_1_3524.20220.1412.2 2 23 1 16.24 9.45 8.72 6.79 90.2 7 1 1 451000.0 Hydrogen 39 (G) 9 VYGFLRNGF (-) 9 VYGFMMLVL V/Y|G|F|L|R\N G\F 77.8 7 6 5 4 0 dual b/y bias 69.852547 0.868611 536.7818 1072.557 -0.0007 -0.7 1.7 8.72 5094.7 nuORFdb_human ENST00000343327.6_1_9:115030452-115095785:- ENST00000343327.6_1_9:115030452-115095785:- PTBP3 5' Overlap uORF 5' Overlap uORF GN=PTBP3 5' Overlap uORF E_20221201_NO30_400nL_HLAc1_4e7_PDAC_Slot2-6_1_3524 Exploris_FAIMS VH20221207_MKP_FAIMS2022E_HLAc1_4e7_PDAC.58816.59101.2 2 2 1 12.75 9.76 4.57 2.99 86.6 7 1 1 1030000000.0 Hydrogen 39 (G) 9 VYGFLRNGF (-) 9 VYGFMMLVL V/Y|G|F/L/R-N G/F 77.8 7 2 6 2 1 y-ion bias 0.999 96.7 102.58 0.63 -50.0 536.7811 1072.557 -0.0025 -2.4 0.20000000000000018 8.72 5094.7 nuORFdb_human ENST00000343327.6_1_9:115030452-115095785:- ENST00000343327.6_1_9:115030452-115095785:- PTBP3 5' Overlap uORF 5' Overlap uORF GN=PTBP3 5' Overlap uORF VH20221207_MKP_FAIMS2022E_HLAc1_4e7_PDAC Eva-50mio-3fr AL20210827_EV_HLA_PDAC_PANFRO413_2D_50e6_W632_classI_fxn3.31649.31649.2 2 1 1 8.34 8.34 8.34 51.3 8 0 0 7570000.0 Hydrogen 31 (K) 9 VYISHGTFF (F) 9 V/Y\I/S/H-G-T/F/F 100.0 9 1 5 0 2 y-ion bias 0.972 64.6 58.08 65.35 0.0 535.7687 1070.531 -0.0004 -0.4 -0.9 6.71 6821.1 nuORFdb_human ENST00000443864.6_1_7:44664063-44684998:+ ENST00000443864.6_1_7:44664063-44684998:+ OGDH Out-of-Frame Out-of-Frame GN=OGDH Out-of-Frame AL20210827_EV_HLA_PDAC_PANFRO413_2D_50e6_W632_classI_fxn3 Eva-50mio-3fr AL20210827_EV_HLA_PDAC_PANFRO413_2D_50e6_W632_classI_fxn2.22350.22350.2 2 1 1 13.62 7.72 9.7 5.9 76.9 7 1 1 1820000.0 Hydrogen 28 (H) 9 VYKLCTQTI (-) 9 VYCPILFTL V/Y|K L\C\T\Q|T/I 77.8 7 5 4 2 0 unbiased Carbamidomethylation C:Carbamidomethylation, 0.155 100.0 42.08 100.0 0.0 563.3025 1125.597 0.0004 0.4 0.10000000000000003 8.56 4450.0 nuORFdb_human ENST00000570041.1_2_16:53532500-53534232:- ENST00000570041.1_2_16:53532500-53534232:- AKTIP 5' Overlap uORF 5' Overlap uORF GN=AKTIP 5' Overlap uORF AL20210827_EV_HLA_PDAC_PANFRO413_2D_50e6_W632_classI_fxn2 SCP E_20221201_NO30_400nL_HLAc1_4e7_PDAC_Slot2-6_1_3524.13339.1359.2 2 2 1 13.03 2.85 9.34 10.18 69.4 8 1 1 12800.0 Hydrogen 28 (H) 9 VYKLCTQTI (-) 9 NPHLCLKMI V/Y|K/L/C|T\Q\T\I 100.0 9 5 5 2 0 unbiased Carbamidomethylation C:Carbamidomethylation, 46.083168 0.901752 563.3006 1125.597 -0.0025 -2.3 0.10000000000000009 8.56 4450.0 nuORFdb_human ENST00000570041.1_2_16:53532500-53534232:- ENST00000570041.1_2_16:53532500-53534232:- AKTIP 5' Overlap uORF 5' Overlap uORF GN=AKTIP 5' Overlap uORF E_20221201_NO30_400nL_HLAc1_4e7_PDAC_Slot2-6_1_3524 Eva-50mio-3fr AL20210827_EV_HLA_PDAC_PANFRO413_2D_50e6_W632_classI_fxn3.33471.33471.2 2 1 1 7.79 5.74 6.1 2.05 69.6 5 0 1 7760000.0 Hydrogen 45 (C) 9 VYKPVSIYL (Y) 9 TKVPFTLYL V Y/K P V-S\I|Y/L 44.4 4 2 3 1 1 unbiased 0.894 100.0 61.28 100.0 0.0 541.3196 1081.629 0.0027 2.5 2.0 8.47 10729.5 nuORFdb_human ENST00000367210.2_2_1:203771435-203771690:+ ENST00000367210.2_2_1:203771435-203771690:+ ZC3H11A 5' uORF 5' uORF GN=ZC3H11A 5' uORF AL20210827_EV_HLA_PDAC_PANFRO413_2D_50e6_W632_classI_fxn3 SCP E_20221201_NO30_400nL_HLAc1_4e7_PDAC_Slot2-6_1_3524.28251.123.1 1 1 1 18.0 5.7 13.4 12.3 93.1 7 1 1 24500.0 Hydrogen 9 (M) 9 VYLMLKMSL (V) 9 FTTKLHTHL V Y|L|M|L|K\M\S\L 77.8 7 7 4 4 0 dual b/y bias 97.60215 1.648279 1097.606 1097.61 0.0003 0.2 2.6 8.56 2354.0 nuORFdb_human ENST00000402538.7_1_2:33740127-33740184:+ ENST00000402538.7_1_2:33740127-33740184:+ RASGRP3 5' uORF 5' uORF GN=RASGRP3 5' uORF E_20221201_NO30_400nL_HLAc1_4e7_PDAC_Slot2-6_1_3524 Eva-50mio-3fr AL20210827_EV_HLA_PDAC_PANFRO413_2D_50e6_W632_classI_fxn3.40673.40673.2 2 1 1 8.99 3.81 4.57 5.18 75.4 7 1 1 11700000.0 M12m Hydrogen 9 (M) 9 VYLmLKMSL (V) 9 YVLCVVTIF V Y|L/m/L-K-M/S/L 77.8 7 1 5 1 2 y-ion bias m:Oxidized methionine 0.981 100.0 73.68 100.0 0.0 557.3067 1097.61 15.9964 1.3 0.8 8.56 2354.0 nuORFdb_human ENST00000402538.7_1_2:33740127-33740184:+ ENST00000402538.7_1_2:33740127-33740184:+ RASGRP3 5' uORF 5' uORF GN=RASGRP3 5' uORF AL20210827_EV_HLA_PDAC_PANFRO413_2D_50e6_W632_classI_fxn3 Exploris AL20221201_MKP_PDAC_01.57821.57821.2 2 1 1 7.91 7.91 7.91 56.0 7 1 1 48200000.0 M12m Hydrogen 9 (M) 9 VYLmLKMSL (V) 9 V Y|L/m/L/K-M/S/L 77.8 7 1 6 1 1 y-ion bias m:Oxidized methionine 0.906 74.7 87.52 100.0 0.0 557.3049 1097.61 15.9928 -1.9 -2.7 8.56 2354.0 nuORFdb_human ENST00000402538.7_1_2:33740127-33740184:+ ENST00000402538.7_1_2:33740127-33740184:+ RASGRP3 5' uORF 5' uORF GN=RASGRP3 5' uORF AL20221201_MKP_PDAC_01 SCP E_20221201_NO30_400nL_HLAc1_4e7_PDAC_Slot2-6_1_3524.27550.1367.2 2 3 1 12.92 5.62 7.65 7.3 62.9 7 1 1 37400.0 M12m Hydrogen 9 (M) 9 VYLmLKMSL (V) 9 VYLLFLAQm V Y|L|m|L|K/M\S\L 77.8 7 6 5 4 0 dual b/y bias m:Oxidized methionine 95.180275 0.896767 557.3034 1097.61 15.989 -5.3 -2.9 8.56 2354.0 nuORFdb_human ENST00000402538.7_1_2:33740127-33740184:+ ENST00000402538.7_1_2:33740127-33740184:+ RASGRP3 5' uORF 5' uORF GN=RASGRP3 5' uORF E_20221201_NO30_400nL_HLAc1_4e7_PDAC_Slot2-6_1_3524 Exploris_FAIMS VH20221207_MKP_FAIMS2022E_HLAc1_4e7_PDAC.60170.60170.2 2 2 1 11.22 1.03 6.29 10.19 71.6 7 1 1 171000000.0 M12m Hydrogen 9 (M) 9 VYLmLKMSL (V) 9 IYPYYVKAP V Y|L/m/L/K-M/S/L 77.8 7 1 6 1 1 y-ion bias m:Oxidized methionine 0.972 88.2 104.5 12.67 -50.0 557.3048 1097.61 15.9925 -2.1 0.5 8.56 2354.0 nuORFdb_human ENST00000402538.7_1_2:33740127-33740184:+ ENST00000402538.7_1_2:33740127-33740184:+ RASGRP3 5' uORF 5' uORF GN=RASGRP3 5' uORF VH20221207_MKP_FAIMS2022E_HLAc1_4e7_PDAC Exploris AL20221201_MKP_PDAC_01.39395.39686.2 2 1 1 8.98 8.98 8.98 53.1 7 1 1 72900000.0 M12m M15m Hydrogen 9 (M) 9 VYLmLKmSL (V) 9 V Y|L|m/L/K-m/S/L 77.8 7 2 6 2 1 y-ion bias m:Oxidized methionine 0.995 81.8 61.17 86.94 0.0 565.3048 1097.61 31.9926 2.4 1.5999999999999999 8.56 2354.0 nuORFdb_human ENST00000402538.7_1_2:33740127-33740184:+ ENST00000402538.7_1_2:33740127-33740184:+ RASGRP3 5' uORF 5' uORF GN=RASGRP3 5' uORF AL20221201_MKP_PDAC_01 SCP E_20221201_NO30_400nL_HLAc1_4e7_PDAC_Slot2-6_1_3524.18371.1352.2 2 2 1 16.99 4.42 9.79 12.57 81.1 8 1 1 6690.0 M12m M15m Hydrogen 9 (M) 9 VYLmLKmSL (V) 9 VYVNHEKQL V/Y|L|m|L|K\m\S\L 100.0 9 7 5 4 0 dual b/y bias m:Oxidized methionine 63.46563 0.906444 565.3036 1097.61 31.991 1.0 3.4 8.56 2354.0 nuORFdb_human ENST00000402538.7_1_2:33740127-33740184:+ ENST00000402538.7_1_2:33740127-33740184:+ RASGRP3 5' uORF 5' uORF GN=RASGRP3 5' uORF E_20221201_NO30_400nL_HLAc1_4e7_PDAC_Slot2-6_1_3524 Exploris_FAIMS VH20221207_MKP_FAIMS2022E_HLAc1_4e7_PDAC.45233.45469.2 2 1 1 11.79 3.28 7.76 8.51 63.5 7 1 1 47400000.0 M12m M15m Hydrogen 9 (M) 9 VYLmLKmSL (V) 9 mLYVPKTYV V Y|L|m/L/K/m/S/L 77.8 7 2 7 2 0 y-ion bias m:Oxidized methionine 0.994 85.9 82.03 27.8 -50.0 565.3022 1097.61 31.9875 -2.1 0.5 8.56 2354.0 nuORFdb_human ENST00000402538.7_1_2:33740127-33740184:+ ENST00000402538.7_1_2:33740127-33740184:+ RASGRP3 5' uORF 5' uORF GN=RASGRP3 5' uORF VH20221207_MKP_FAIMS2022E_HLAc1_4e7_PDAC SCP E_20221201_NO30_400nL_HLAc1_4e7_PDAC_Slot2-6_1_3524.16729.339.1 1 1 1 9.66 6.37 5.8 3.3 70.1 6 1 1 5450.0 Hydrogen 144 (R) 9 VYLPAAKTF (I) 9 LSPPPAALPF V Y/L|P A/A|K\T\F 55.6 5 4 4 2 0 unbiased 57.793541 1.521639 1009.5683 1009.572 -0.0017 -1.7 0.7 8.56 24601.4 nuORFdb_human ENST00000608354.1_1_22:30642226-30642904:+ ENST00000608354.1_1_22:30642226-30642904:+ RP1-102K2.8 Antisense lncRNA GN=RP1-102K2.8 Antisense E_20221201_NO30_400nL_HLAc1_4e7_PDAC_Slot2-6_1_3524 Exploris_FAIMS VH20221207_MKP_FAIMS2022E_HLAc1_4e7_PDAC.42552.42552.2 2 2 1 14.8 5.86 10.18 8.95 87.0 7 1 1 68100000.0 Hydrogen 144 (R) 9 VYLPAAKTF (I) 9 KKETFIPF V Y|L/P/A/A/K/T/F 77.8 7 1 7 1 0 y-ion bias 0.99 72.3 77.65 19.66 -50.0 505.2883 1009.572 -0.0024 -2.3 0.30000000000000027 8.56 24601.4 nuORFdb_human ENST00000608354.1_1_22:30642226-30642904:+ ENST00000608354.1_1_22:30642226-30642904:+ RP1-102K2.8 Antisense lncRNA GN=RP1-102K2.8 Antisense VH20221207_MKP_FAIMS2022E_HLAc1_4e7_PDAC SCP E_20221201_NO30_400nL_HLAc1_4e7_PDAC_Slot2-6_1_3524.11515.1327.2 2 1 1 17.4 8.44 9.84 8.96 94.6 8 1 1 9170.0 Hydrogen 27 (S) 9 VYMVVSRHL (-) 9 VMYVVSLRH V/Y|M|V|V/S/R\H\L 100.0 9 5 6 3 0 dual b/y bias 39.7817 0.921866 552.3036 1103.603 -0.0023 -2.1 0.2999999999999998 8.73 4203.0 nuORFdb_human ENST00000481195.5_1_5:133536728-133537609:- ENST00000481195.5_1_5:133536728-133537609:- PPP2CA Out-of-Frame Out-of-Frame GN=PPP2CA Out-of-Frame E_20221201_NO30_400nL_HLAc1_4e7_PDAC_Slot2-6_1_3524 Exploris_FAIMS VH20221207_MKP_FAIMS2022E_HLAc1_4e7_PDAC.22854.22854.3 3 1 1 11.98 4.29 7.25 7.69 74.8 6 2 2 16200000.0 Hydrogen 7 (H) 10 VYNKSCRVEF (L) 10 EFGnKYWLML V/Y/N/K/S/C R V E/F 60.0 6 0 6 0 0 y-ion bias Carbamidomethylation C:Carbamidomethylation, 1.0 100.0 47.53 24.38 -70.0 434.5472 1301.631 -0.0036 -2.8 -0.19999999999999973 8.56 4106.6 smORF_v4|nuORFdb_human LINC00857_10:81967517-81967620:+ LINC00857_10:81967517-81967620:+|ENST00000422847.1_1_10:81967506-81967617:+ LINC00857 lncRNA 10:81967517-81967620:+ 10 LINC00857 lincRNA lncRNA GN=LINC00857 VH20221207_MKP_FAIMS2022E_HLAc1_4e7_PDAC SCP E_20221201_NO30_400nL_HLAc1_4e7_PDAC_Slot2-6_1_3524.8981.1278.2 2 1 1 14.47 9.48 7.17 4.98 87.9 7 1 1 9680.0 Hydrogen 5 (L) 9 VYQRALCHF (H) 9 SAFQIALCHF V/Y/Q R/A\L|C|H\F 77.8 7 4 5 2 0 unbiased Carbamidomethylation C:Carbamidomethylation, 31.027065 0.952487 597.2951 1193.588 -0.0057 -4.8 -2.4 8.73 2816.2 nuORFdb_human ENST00000378344.6_1_1:3544126-3544195:+ ENST00000378344.6_1_1:3544126-3544195:+ TPRG1L Out-of-Frame Out-of-Frame GN=TPRG1L Out-of-Frame E_20221201_NO30_400nL_HLAc1_4e7_PDAC_Slot2-6_1_3524 Exploris_FAIMS VH20221207_MKP_FAIMS2022E_HLAc1_4e7_PDAC.25509.25509.3 3 1 1 10.27 5.56 6.66 4.71 74.2 6 1 1 19900000.0 Hydrogen 5 (L) 9 VYQRALCHF (H) 9 VnmKLKCHF V/Y Q R-A-L/C/H/F 66.7 6 0 4 0 2 y-ion bias Carbamidomethylation C:Carbamidomethylation, 1.0 96.9 52.0 16.48 -50.0 398.5334 1193.588 -0.0029 -2.4 0.20000000000000018 8.73 2816.2 nuORFdb_human ENST00000378344.6_1_1:3544126-3544195:+ ENST00000378344.6_1_1:3544126-3544195:+ TPRG1L Out-of-Frame Out-of-Frame GN=TPRG1L Out-of-Frame VH20221207_MKP_FAIMS2022E_HLAc1_4e7_PDAC SCP E_20221201_NO30_400nL_HLAc1_4e7_PDAC_Slot2-6_1_3524.2611.1646.3 3 3 1 12.08 7.39 5.19 4.68 92.4 6 2 2 116000.0 Hydrogen 28 (M) 9 VYVKHQRNL (-) 9 VYVTALTHRP V/Y|V/K/H Q/R\N L 55.6 5 2 5 1 0 y-ion bias 9.029467 0.720927 386.2216 1156.659 -0.0071 -6.1 -3.6999999999999997 9.99 4340.1 nuORFdb_human ENST00000634733.1_1_6:26224458-26225162:+ ENST00000634733.1_1_6:26224458-26225162:+ HIST1H3E 5' uORF 5' uORF GN=HIST1H3E 5' uORF E_20221201_NO30_400nL_HLAc1_4e7_PDAC_Slot2-6_1_3524 Exploris_FAIMS VH20221207_MKP_FAIMS2022E_HLAc1_4e7_PDAC.10684.10684.3 3 1 1 10.5 4.41 5.85 6.09 76.3 5 2 2 7430000.0 Hydrogen 28 (M) 9 VYVKHQRNL (-) 9 VRYHLPKML V/Y|V/K/H Q/R N L 44.4 4 1 5 1 0 y-ion bias 0.989 95.8 22.97 120.0 -50.0 386.2232 1156.659 -0.0034 -3.0 -0.3999999999999999 9.99 4340.1 nuORFdb_human ENST00000634733.1_1_6:26224458-26225162:+ ENST00000634733.1_1_6:26224458-26225162:+ HIST1H3E 5' uORF 5' uORF GN=HIST1H3E 5' uORF VH20221207_MKP_FAIMS2022E_HLAc1_4e7_PDAC 10mio-rep1 E_20221201_NO30_400nL_HLAc1_1e7_directIP_titration_rep1_Slot2-3_1_3512.15885.1519.2 2 1 1 8.57 5.48 2.11 3.09 65.1 5 0 1 3060.0 Hydrogen 48 (C) 9 WAAPSRPPL (-) 9 AWALHLLGGG W/A|A|P/S R/P P L 44.4 4 2 5 2 0 y-ion bias 54.875607 0.801705 497.7712 994.547 -0.0122 -12.2 -4.499999999999999 9.75 5807.6 nuORFdb_human ENST00000520220.6_2_8:56792441-56792612:+ ENST00000520220.6_2_8:56792441-56792612:+ LYN 5' uORF 5' uORF GN=LYN 5' uORF E_20221201_NO30_400nL_HLAc1_1e7_directIP_titration_rep1_Slot2-3_1_3512 40mio-rep1 E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep1_Slot1-04_1_3496.16021.1491.2 2 2 1 9.14 6.35 3.33 2.79 76.2 6 0 1 16200.0 Hydrogen 48 (C) 9 WAAPSRPPL (-) 9 AWALHLLGGG W/A|A|P/S R/P P\L 55.6 5 3 5 2 0 unbiased 55.341324 0.818972 497.7744 994.547 -0.0062 -6.2 -3.0 9.75 5807.6 nuORFdb_human ENST00000520220.6_2_8:56792441-56792612:+ ENST00000520220.6_2_8:56792441-56792612:+ LYN 5' uORF 5' uORF GN=LYN 5' uORF E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep1_Slot1-04_1_3496 40mio-rep2 E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep2_Slot2-5_1_3520.15740.1506.2 2 1 1 8.78 5.46 2.75 3.32 79.8 5 0 1 7760.0 Hydrogen 48 (C) 9 WAAPSRPPL (-) 9 AWALHLLGGG W/A|A|P S/R/P P L 44.4 4 2 5 2 0 y-ion bias 54.372211 0.809737 497.7741 994.547 -0.0062 -6.2 -1.2999999999999998 9.75 5807.6 nuORFdb_human ENST00000520220.6_2_8:56792441-56792612:+ ENST00000520220.6_2_8:56792441-56792612:+ LYN 5' uORF 5' uORF GN=LYN 5' uORF E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep2_Slot2-5_1_3520 40mio-rep4 E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep4_Slot2-5_1_3522.15740.1503.2 2 1 1 8.33 6.47 1.82 1.85 76.9 5 0 1 5300.0 Hydrogen 48 (C) 9 WAAPSRPPL (-) 9 AWALHLLGGG W/A|A|P/S-R P P L 55.6 5 2 4 2 1 y-ion bias 54.358665 0.811396 497.7745 994.547 -0.0042 -4.2 0.5 9.75 5807.6 nuORFdb_human ENST00000520220.6_2_8:56792441-56792612:+ ENST00000520220.6_2_8:56792441-56792612:+ LYN 5' uORF 5' uORF GN=LYN 5' uORF E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep4_Slot2-5_1_3522 20mio-rep1 E_20221201_NO30_400nL_HLAc1_2e7_directIP_titration_rep1_Slot2-4_1_3515.28420.1364.2 2 1 1 9.73 1.64 10.94 8.09 63.5 7 0 0 4760.0 Hydrogen 4 (Y) 10 WENLFSGTEW (R) 10 mSTPSSTPGTTW W E\N\L|F|S|G T\E\W 60.0 6 7 3 3 0 b-ion bias 98.137367 0.899073 634.7799 1268.558 -0.0055 -4.3 1.9000000000000004 3.8 2438.7 smORF_v4|nuORFdb_human RNF41_12:56610245-56610308:- RNF41_12:56610245-56610308:-|ENST00000547967.5_1_12:56610242-56610374:- RNF41 uORF 12:56610245-56610308:- 12 RNF41 5' uORF 5' uORF GN=RNF41 E_20221201_NO30_400nL_HLAc1_2e7_directIP_titration_rep1_Slot2-4_1_3515 20mio-rep2 E_20221201_NO30_400nL_HLAc1_2e7_directIP_titration_rep3_Slot2-4_1_3518.28425.1367.2 2 1 1 8.46 1.91 6.33 6.55 64.3 6 0 0 3630.0 Hydrogen 4 (Y) 10 WENLFSGTEW (R) 10 PTACLFSAWES W E N\L|F|S|G T/E\W 50.0 5 5 4 3 0 unbiased 98.176842 0.896716 634.7788 1268.558 -0.0075 -5.9 0.39999999999999947 3.8 2438.7 smORF_v4|nuORFdb_human RNF41_12:56610245-56610308:- RNF41_12:56610245-56610308:-|ENST00000547967.5_1_12:56610242-56610374:- RNF41 uORF 12:56610245-56610308:- 12 RNF41 5' uORF 5' uORF GN=RNF41 E_20221201_NO30_400nL_HLAc1_2e7_directIP_titration_rep3_Slot2-4_1_3518 20mio-rep3 E_20221201_NO30_400nL_HLAc1_2e7_directIP_titration_rep4_Slot2-4_1_3519.28431.1366.2 2 1 1 11.82 6.56 7.97 5.25 78.3 6 0 0 2100.0 Hydrogen 4 (Y) 10 WENLFSGTEW (R) 10 PTACLFSAWES W E\N\L|F|S|G T E\W 50.0 5 6 3 3 0 b-ion bias 98.194702 0.897916 634.7817 1268.558 -0.0015 -1.2 4.6 3.8 2438.7 smORF_v4|nuORFdb_human RNF41_12:56610245-56610308:- RNF41_12:56610245-56610308:-|ENST00000547967.5_1_12:56610242-56610374:- RNF41 uORF 12:56610245-56610308:- 12 RNF41 5' uORF 5' uORF GN=RNF41 E_20221201_NO30_400nL_HLAc1_2e7_directIP_titration_rep4_Slot2-4_1_3519 40mio-rep1 E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep1_Slot1-04_1_3496.28510.147.1 1 2 1 17.81 6.23 13.45 11.58 95.6 8 0 0 9800.0 Hydrogen 4 (Y) 10 WENLFSGTEW (R) 10 PTACLFSAWES W E/N|L|F|S|G\T\E\W 80.0 8 7 5 4 0 dual b/y bias 98.49395 1.63398 1268.5525 1268.558 -0.0082 -6.5 -3.3 3.8 2438.7 smORF_v4|nuORFdb_human RNF41_12:56610245-56610308:- RNF41_12:56610245-56610308:-|ENST00000547967.5_1_12:56610242-56610374:- RNF41 uORF 12:56610245-56610308:- 12 RNF41 5' uORF 5' uORF GN=RNF41 E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep1_Slot1-04_1_3496 40mio-rep3 E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep3_Slot2-5_1_3521.28427.1358.2 2 2 1 17.27 6.11 11.32 11.16 92.0 8 0 0 14400.0 Hydrogen 4 (Y) 10 WENLFSGTEW (R) 10 SAISPSmSSAMSP W E\N\L|F|S|G/T|E\W 80.0 8 7 5 4 0 dual b/y bias 98.195473 0.902809 634.7804 1268.558 -0.0055 -4.3 0.2999999999999998 3.8 2438.7 smORF_v4|nuORFdb_human RNF41_12:56610245-56610308:- RNF41_12:56610245-56610308:-|ENST00000547967.5_1_12:56610242-56610374:- RNF41 uORF 12:56610245-56610308:- 12 RNF41 5' uORF 5' uORF GN=RNF41 E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep3_Slot2-5_1_3521 40mio-rep4 E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep4_Slot2-5_1_3522.28437.1360.2 2 2 1 12.7 4.22 9.4 8.48 77.4 7 0 0 19400.0 Hydrogen 4 (Y) 10 WENLFSGTEW (R) 10 PTACLFSAWES W E\N L|F|S|G/T|E\W 60.0 6 6 5 4 0 dual b/y bias 98.2202 0.901527 634.7805 1268.558 -0.0035 -2.8 1.9000000000000004 3.8 2438.7 smORF_v4|nuORFdb_human RNF41_12:56610245-56610308:- RNF41_12:56610245-56610308:-|ENST00000547967.5_1_12:56610242-56610374:- RNF41 uORF 12:56610245-56610308:- 12 RNF41 5' uORF 5' uORF GN=RNF41 E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep4_Slot2-5_1_3522 40mio-rep1 E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep1_Slot1-04_1_3496.26701.374.1 1 1 1 8.01 4.25 5.01 3.76 66.7 6 0 0 5810.0 Hydrogen 2 (M) 9 WMSELISGV (-) 9 SWMESIIR W M/S\E\L\I\S\G V 55.6 5 5 1 0 0 b-ion bias 92.242409 1.500754 1021.4983 1021.502 -0.0023 -2.2 1.0 4.0 1152.4 nuORFdb_human ENST00000440496.5_1_X:102100790-102100820:+ ENST00000440496.5_1_X:102100790-102100820:+ LINC00630 lincRNA lncRNA GN=LINC00630 lincRNA E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep1_Slot1-04_1_3496 SCP E_20221201_NO30_400nL_HLAc1_4e7_PDAC_Slot2-6_1_3524.12769.1278.2 2 2 1 8.44 3.21 0.72 5.22 61.6 7 1 2 15800.0 Hydrogen 25 (S) 9 WPKREDYSF (I) 9 FRSmTSSLPW W/P/K/R E\D/Y/S\F 77.8 7 2 5 0 0 y-ion bias 44.114944 0.952235 614.2923 1227.579 -0.0026 -2.1 0.2999999999999998 6.07 4721.6 nuORFdb_human ENST00000431877.6_1_2:182761670-182763799:+ ENST00000431877.6_1_2:182761670-182763799:+ SSFA2 Out-of-Frame Out-of-Frame GN=SSFA2 Out-of-Frame E_20221201_NO30_400nL_HLAc1_4e7_PDAC_Slot2-6_1_3524 1mio-rep1 E_20221201_NO30_400nL_HLAc1_1e6_directIP_titration_rep1_Slot1-06_1_3498.23574.294.1 1 4 1 15.13 3.07 12.55 12.06 90.7 6 0 0 5510.0 Hydrogen 12 (V) 9 WQDAELGLY (F) 9 LENQNSGAVY W Q|D A|E|L|G\L\Y 55.6 5 6 4 4 0 dual b/y bias 81.411812 1.54763 1094.5072 1094.515 -0.0053 -4.8 0.10000000000000053 3.67 3793.1 nuORFdb_human ENST00000568282.1_1_16:29819940-29820048:+ ENST00000568282.1_1_16:29819940-29820048:+ MAZ 5' Overlap uORF 5' Overlap uORF GN=MAZ 5' Overlap uORF E_20221201_NO30_400nL_HLAc1_1e6_directIP_titration_rep1_Slot1-06_1_3498 10mio-rep1 E_20221201_NO30_400nL_HLAc1_1e7_directIP_titration_rep1_Slot2-3_1_3512.23347.1418.2 2 5 1 14.94 9.26 6.17 5.68 86.4 6 0 0 17600.0 Hydrogen 12 (V) 9 WQDAELGLY (F) 9 DVARDDAGLY W Q\D A|E|L|G|L\Y 55.6 5 6 4 4 0 dual b/y bias 80.64592 0.865244 547.7564 1094.515 -0.0106 -9.7 -1.9999999999999991 3.67 3793.1 nuORFdb_human ENST00000568282.1_1_16:29819940-29820048:+ ENST00000568282.1_1_16:29819940-29820048:+ MAZ 5' Overlap uORF 5' Overlap uORF GN=MAZ 5' Overlap uORF E_20221201_NO30_400nL_HLAc1_1e7_directIP_titration_rep1_Slot2-3_1_3512 10mio-rep2 E_20221201_NO30_400nL_HLAc1_1e7_directIP_titration_rep2_Slot2-3_1_3513.23283.1420.2 2 6 1 13.19 8.63 3.51 4.55 84.7 5 0 0 21000.0 Hydrogen 12 (V) 9 WQDAELGLY (F) 9 FcLFLGIY W Q\D A E|L|G|L\Y 44.4 4 5 3 3 0 dual b/y bias 80.418259 0.863904 547.7574 1094.515 -0.0086 -7.8 -1.0999999999999996 3.67 3793.1 nuORFdb_human ENST00000568282.1_1_16:29819940-29820048:+ ENST00000568282.1_1_16:29819940-29820048:+ MAZ 5' Overlap uORF 5' Overlap uORF GN=MAZ 5' Overlap uORF E_20221201_NO30_400nL_HLAc1_1e7_directIP_titration_rep2_Slot2-3_1_3513 10mio-rep3 E_20221201_NO30_400nL_HLAc1_1e7_directIP_titration_rep3_Slot2-3_1_3514.23237.1425.2 2 2 1 14.79 10.41 5.25 4.38 87.8 5 0 0 7670.0 Hydrogen 12 (V) 9 WQDAELGLY (F) 9 FcLFLGIY W Q D A\E|L|G|L\Y 55.6 5 5 3 3 0 dual b/y bias 80.267334 0.860388 547.7565 1094.515 -0.0086 -7.8 -1.5 3.67 3793.1 nuORFdb_human ENST00000568282.1_1_16:29819940-29820048:+ ENST00000568282.1_1_16:29819940-29820048:+ MAZ 5' Overlap uORF 5' Overlap uORF GN=MAZ 5' Overlap uORF E_20221201_NO30_400nL_HLAc1_1e7_directIP_titration_rep3_Slot2-3_1_3514 20mio-rep1 E_20221201_NO30_400nL_HLAc1_2e7_directIP_titration_rep1_Slot2-4_1_3515.23233.1413.2 2 5 1 15.08 8.86 6.49 6.22 85.9 6 0 0 45300.0 Hydrogen 12 (V) 9 WQDAELGLY (F) 9 FcLFLGIY W Q\D A\E|L|G|L\Y 55.6 5 6 3 3 0 dual b/y bias 80.230446 0.868348 547.7592 1094.515 -0.0046 -4.2 2.0 3.67 3793.1 nuORFdb_human ENST00000568282.1_1_16:29819940-29820048:+ ENST00000568282.1_1_16:29819940-29820048:+ MAZ 5' Overlap uORF 5' Overlap uORF GN=MAZ 5' Overlap uORF E_20221201_NO30_400nL_HLAc1_2e7_directIP_titration_rep1_Slot2-4_1_3515 20mio-rep2 E_20221201_NO30_400nL_HLAc1_2e7_directIP_titration_rep3_Slot2-4_1_3518.23289.1409.2 2 5 1 15.38 9.03 6.28 6.35 86.4 6 0 0 66500.0 Hydrogen 12 (V) 9 WQDAELGLY (F) 9 FcLFLGIY W Q D\A\E|L|G|L\Y 66.7 6 6 3 3 0 dual b/y bias 80.440636 0.870921 547.7572 1094.515 -0.0086 -7.8 -1.5 3.67 3793.1 nuORFdb_human ENST00000568282.1_1_16:29819940-29820048:+ ENST00000568282.1_1_16:29819940-29820048:+ MAZ 5' Overlap uORF 5' Overlap uORF GN=MAZ 5' Overlap uORF E_20221201_NO30_400nL_HLAc1_2e7_directIP_titration_rep3_Slot2-4_1_3518 20mio-rep3 E_20221201_NO30_400nL_HLAc1_2e7_directIP_titration_rep4_Slot2-4_1_3519.23288.299.1 1 3 1 17.24 2.36 14.7 14.88 95.3 7 0 0 24200.0 Hydrogen 12 (V) 9 WQDAELGLY (F) 9 NAEDIEQLY W Q|D/A|E|L\G\L\Y 77.8 7 6 4 3 0 dual b/y bias 80.42852 1.545091 1094.5146 1094.515 -0.0053 -4.8 1.0 3.67 3793.1 nuORFdb_human ENST00000568282.1_1_16:29819940-29820048:+ ENST00000568282.1_1_16:29819940-29820048:+ MAZ 5' Overlap uORF 5' Overlap uORF GN=MAZ 5' Overlap uORF E_20221201_NO30_400nL_HLAc1_2e7_directIP_titration_rep4_Slot2-4_1_3519 40mio-rep1 E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep1_Slot1-04_1_3496.23487.1379.2 2 6 1 15.39 7.22 7.02 8.17 87.1 6 0 0 222000.0 Hydrogen 12 (V) 9 WQDAELGLY (F) 9 DVARDDAGLY W Q\D A\E|L\G|L\Y 55.6 5 6 2 2 0 b-ion bias 81.137543 0.889286 547.7595 1094.515 -0.0026 -2.4 0.8000000000000003 3.67 3793.1 nuORFdb_human ENST00000568282.1_1_16:29819940-29820048:+ ENST00000568282.1_1_16:29819940-29820048:+ MAZ 5' Overlap uORF 5' Overlap uORF GN=MAZ 5' Overlap uORF E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep1_Slot1-04_1_3496 40mio-rep2 E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep2_Slot2-5_1_3520.23224.1398.2 2 5 1 13.53 8.17 4.76 5.35 74.2 6 0 0 93000.0 Hydrogen 12 (V) 9 WQDAELGLY (F) 9 DVARDDAGLY W Q\D A\E|L|G|L\Y 55.6 5 6 3 3 0 dual b/y bias 80.227859 0.877452 547.7591 1094.515 -0.0046 -4.2 0.7000000000000002 3.67 3793.1 nuORFdb_human ENST00000568282.1_1_16:29819940-29820048:+ ENST00000568282.1_1_16:29819940-29820048:+ MAZ 5' Overlap uORF 5' Overlap uORF GN=MAZ 5' Overlap uORF E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep2_Slot2-5_1_3520 40mio-rep3 E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep3_Slot2-5_1_3521.23245.1400.2 2 5 1 15.48 8.26 7.73 7.22 88.0 6 0 0 99600.0 Hydrogen 12 (V) 9 WQDAELGLY (F) 9 FcLFLGIY W Q\D A\E|L|G|L\Y 55.6 5 6 3 3 0 dual b/y bias 80.297707 0.876362 547.7593 1094.515 -0.0046 -4.2 0.39999999999999947 3.67 3793.1 nuORFdb_human ENST00000568282.1_1_16:29819940-29820048:+ ENST00000568282.1_1_16:29819940-29820048:+ MAZ 5' Overlap uORF 5' Overlap uORF GN=MAZ 5' Overlap uORF E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep3_Slot2-5_1_3521 40mio-rep4 E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep4_Slot2-5_1_3522.23219.296.1 1 5 1 15.09 1.66 13.21 13.43 94.6 6 0 0 75700.0 Hydrogen 12 (V) 9 WQDAELGLY (F) 9 LENQNSGAVY W Q|D A|E\L\G\L\Y 55.6 5 6 2 2 0 b-ion bias 80.196907 1.546716 1094.5086 1094.515 -0.0053 -4.8 -0.09999999999999964 3.67 3793.1 nuORFdb_human ENST00000568282.1_1_16:29819940-29820048:+ ENST00000568282.1_1_16:29819940-29820048:+ MAZ 5' Overlap uORF 5' Overlap uORF GN=MAZ 5' Overlap uORF E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep4_Slot2-5_1_3522 5mio-rep1 E_20221201_NO30_400nL_HLAc1_5e6_directIP_titration_rep1_Slot1-37_1_3502.23546.305.1 1 2 1 13.74 1.53 12.73 12.21 90.5 6 0 0 5030.0 Hydrogen 12 (V) 9 WQDAELGLY (F) 9 FcLFLGIY W Q|D A\E|L\G\L\Y 55.6 5 6 2 2 0 b-ion bias 81.326042 1.54148 1094.5127 1094.515 -0.0053 -4.8 2.2 3.67 3793.1 nuORFdb_human ENST00000568282.1_1_16:29819940-29820048:+ ENST00000568282.1_1_16:29819940-29820048:+ MAZ 5' Overlap uORF 5' Overlap uORF GN=MAZ 5' Overlap uORF E_20221201_NO30_400nL_HLAc1_5e6_directIP_titration_rep1_Slot1-37_1_3502 5mio-rep2 E_20221201_NO30_400nL_HLAc1_5e6_directIP_titration_rep2_Slot2-2_1_3510.23442.313.1 1 2 1 13.08 1.96 9.71 11.11 89.3 6 0 0 3340.0 Hydrogen 12 (V) 9 WQDAELGLY (F) 9 LENQNSGAVY W Q|D A|E\L\G\L\Y 55.6 5 6 2 2 0 b-ion bias 80.958969 1.536607 1094.5108 1094.515 -0.0053 -4.8 3.8999999999999995 3.67 3793.1 nuORFdb_human ENST00000568282.1_1_16:29819940-29820048:+ ENST00000568282.1_1_16:29819940-29820048:+ MAZ 5' Overlap uORF 5' Overlap uORF GN=MAZ 5' Overlap uORF E_20221201_NO30_400nL_HLAc1_5e6_directIP_titration_rep2_Slot2-2_1_3510 40mio-rep1 E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep1_Slot1-04_1_3496.29104.1290.2 2 1 1 13.72 9.34 4.53 4.38 87.0 7 1 1 4030.0 Hydrogen 17 (N) 9 WTLKELILW (T) 9 LVnSSRILIW W/T/L/K E\L|I|L\W 77.8 7 4 5 2 0 unbiased 100.544731 0.944877 601.3482 1201.698 -0.0092 -7.7 -4.5 6.0 5369.9 nuORFdb_human T246212_3:87940846-87940975:- T246212_3:87940846-87940975:- lincRNA lncRNA GN=nan lincRNA E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep1_Slot1-04_1_3496 Exploris AL20221201_MKP_PDAC_01.9512.9512.2 2 1 1 11.54 7.66 3.41 3.88 83.1 6 2 2 4390000.0 Hydrogen 4 (S) 8 YAKSTQRF (-) 8 YAKAAKYW Y/A|K/S/T/Q R-F 75.0 6 1 5 1 1 y-ion bias 0.833 90.3 18.12 100.0 0.0 500.764 1000.521 -0.0003 -0.3 -1.1 9.99 1275.4 nuORFdb_human ENST00000430580.6_1_1:16939836-16939869:- ENST00000430580.6_1_1:16939836-16939869:- NBPF1 5' uORF 5' uORF GN=NBPF1 5' uORF AL20221201_MKP_PDAC_01 SCP E_20221201_NO30_400nL_HLAc1_4e7_PDAC_Slot2-6_1_3524.3469.1454.2 2 1 1 11.98 7.89 5.43 4.08 79.0 7 2 2 16100.0 Hydrogen 4 (S) 8 YAKSTQRF (-) 8 AYKSLGQAY Y/A/K|S|T|Q/R\F 100.0 8 4 6 3 0 dual b/y bias 11.997571 0.842724 500.762 1000.521 -0.0043 -4.3 -1.9 9.99 1275.4 nuORFdb_human ENST00000430580.6_1_1:16939836-16939869:- ENST00000430580.6_1_1:16939836-16939869:- NBPF1 5' uORF 5' uORF GN=NBPF1 5' uORF E_20221201_NO30_400nL_HLAc1_4e7_PDAC_Slot2-6_1_3524 Exploris_FAIMS VH20221207_MKP_FAIMS2022E_HLAc1_4e7_PDAC.13608.13608.2 2 1 1 10.34 7.86 2.38 2.48 90.5 5 2 2 667000.0 Hydrogen 4 (S) 8 YAKSTQRF (-) 8 YAKAAKYW Y/A|K/S/T/Q R F 62.5 5 1 5 1 0 y-ion bias 0.995 100.0 29.47 120.0 -50.0 500.7633 1000.521 -0.0018 -1.8 0.8 9.99 1275.4 nuORFdb_human ENST00000430580.6_1_1:16939836-16939869:- ENST00000430580.6_1_1:16939836-16939869:- NBPF1 5' uORF 5' uORF GN=NBPF1 5' uORF VH20221207_MKP_FAIMS2022E_HLAc1_4e7_PDAC 40mio-rep1 E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep1_Slot1-04_1_3496.6328.1636.3 3 3 1 9.28 4.13 4.16 5.15 75.4 7 1 2 79000.0 Hydrogen 8 (E) 9 YFVPHTKRY (-) 9 YFSAAVSRPGR Y/F|V/P/H/T/K R\Y 77.8 7 2 6 1 0 y-ion bias 21.868196 0.727333 404.2151 1210.637 -0.0063 -5.2 -2.0 9.7 2030.3 nuORFdb_human ENST00000419821.6_1_12:118693456-118704503:- ENST00000419821.6_1_12:118693456-118704503:- TAOK3 5' uORF 5' uORF GN=TAOK3 5' uORF E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep1_Slot1-04_1_3496 40mio-rep3 E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep3_Slot2-5_1_3521.6108.1649.3 3 1 1 7.74 3.94 2.2 3.8 78.2 6 1 2 23200.0 Hydrogen 8 (E) 9 YFVPHTKRY (-) 9 YFSAAVSRPGR Y/F|V/P/H/T K R\Y 66.7 6 2 5 1 0 y-ion bias 21.103001 0.719333 404.2144 1210.637 -0.0093 -7.7 -3.1000000000000005 9.7 2030.3 nuORFdb_human ENST00000419821.6_1_12:118693456-118704503:- ENST00000419821.6_1_12:118693456-118704503:- TAOK3 5' uORF 5' uORF GN=TAOK3 5' uORF E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep3_Slot2-5_1_3521 SCP E_20221201_NO30_400nL_HLAc1_4e7_PDAC_Slot2-6_1_3524.23301.1525.2 2 5 1 9.91 5.29 4.06 4.62 76.7 6 1 1 51400.0 Hydrogen 40 (V) 8 YGFLRNGF (-) 8 YGSGKIWY Y/G|F|L/R N\G\F 75.0 6 4 4 2 0 unbiased 80.499214 0.797531 487.2466 973.489 -0.0023 -2.4 0.0 8.75 5094.7 nuORFdb_human ENST00000343327.6_1_9:115030452-115095785:- ENST00000343327.6_1_9:115030452-115095785:- PTBP3 5' Overlap uORF 5' Overlap uORF GN=PTBP3 5' Overlap uORF E_20221201_NO30_400nL_HLAc1_4e7_PDAC_Slot2-6_1_3524 20mio-rep1 E_20221201_NO30_400nL_HLAc1_2e7_directIP_titration_rep1_Slot2-4_1_3515.27809.203.1 1 4 1 11.69 6.55 5.99 5.14 82.8 6 0 0 6470.0 Hydrogen 77 (Y) 10 YLDAAPFSLY (-) 10 nFSLATNTKY Y L D\A/A|P F|S\L\Y 50.0 5 5 3 2 0 unbiased 96.027756 1.601271 1159.5644 1159.567 -0.007 -6.0 0.20000000000000018 3.8 10071.4 nuORFdb_human ENST00000629778.3_1_1:210111609-210187056:+ ENST00000629778.3_1_1:210111609-210187056:+ SYT14 Out-of-Frame Out-of-Frame GN=SYT14 Out-of-Frame E_20221201_NO30_400nL_HLAc1_2e7_directIP_titration_rep1_Slot2-4_1_3515 40mio-rep1 E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep1_Slot1-04_1_3496.27933.165.1 1 2 1 12.57 7.1 6.15 5.47 88.0 6 0 0 8310.0 Hydrogen 77 (Y) 10 YLDAAPFSLY (-) 10 IDTGFSLPFY Y L D A|A|P\F\S\L\Y 60.0 6 6 2 2 0 b-ion bias 96.500229 1.623577 1159.5649 1159.567 -0.007 -6.0 -2.8 3.8 10071.4 nuORFdb_human ENST00000629778.3_1_1:210111609-210187056:+ ENST00000629778.3_1_1:210111609-210187056:+ SYT14 Out-of-Frame Out-of-Frame GN=SYT14 Out-of-Frame E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep1_Slot1-04_1_3496 40mio-rep2 E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep2_Slot2-5_1_3520.27812.1371.2 2 3 1 14.07 5.8 8.86 8.26 85.8 7 0 0 15400.0 Hydrogen 77 (Y) 10 YLDAAPFSLY (-) 10 IDTGFSLPFY Y L D\A|A|P|F\S|L\Y 70.0 7 7 4 4 0 dual b/y bias 96.07959 0.89451 580.2843 1159.567 -0.0063 -5.4 -0.5 3.8 10071.4 nuORFdb_human ENST00000629778.3_1_1:210111609-210187056:+ ENST00000629778.3_1_1:210111609-210187056:+ SYT14 Out-of-Frame Out-of-Frame GN=SYT14 Out-of-Frame E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep2_Slot2-5_1_3520 40mio-rep3 E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep3_Slot2-5_1_3521.27813.1366.2 2 5 1 17.53 5.43 11.63 12.11 91.4 8 0 0 22700.0 Hydrogen 77 (Y) 10 YLDAAPFSLY (-) 10 ISWLSDYIY Y L\D\A|A|P|F\S|L\Y 80.0 8 8 4 4 0 dual b/y bias 96.075607 0.897435 580.2872 1159.567 -0.0003 -0.2 4.3999999999999995 3.8 10071.4 nuORFdb_human ENST00000629778.3_1_1:210111609-210187056:+ ENST00000629778.3_1_1:210111609-210187056:+ SYT14 Out-of-Frame Out-of-Frame GN=SYT14 Out-of-Frame E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep3_Slot2-5_1_3521 40mio-rep4 E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep4_Slot2-5_1_3522.27824.184.1 1 4 1 15.15 7.24 8.4 7.91 93.6 7 0 0 18400.0 Hydrogen 77 (Y) 10 YLDAAPFSLY (-) 10 IDTGFSLPFY Y L D\A|A|P\F|S\L\Y 70.0 7 7 3 3 0 b-ion bias 96.103241 1.612232 1159.5629 1159.567 -0.007 -6.0 -1.2999999999999998 3.8 10071.4 nuORFdb_human ENST00000629778.3_1_1:210111609-210187056:+ ENST00000629778.3_1_1:210111609-210187056:+ SYT14 Out-of-Frame Out-of-Frame GN=SYT14 Out-of-Frame E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep4_Slot2-5_1_3522 10mio-rep2 E_20221201_NO30_400nL_HLAc1_1e7_directIP_titration_rep2_Slot2-3_1_3513.16097.1443.2 2 4 1 14.49 6.23 10.88 8.26 77.5 7 0 0 13500.0 Hydrogen 31 (S) 9 YLDPAQQNL (Y) 9 IYGPEQVQQ Y L|D|P/A|Q|Q|N\L 77.8 7 6 6 5 0 dual b/y bias 55.606716 0.849092 531.2625 1061.526 -0.0095 -8.9 -2.2 3.8 51570.8 nuORFdb_human ENST00000421406.1_1_1:247353282-247372753:- ENST00000421406.1_1_1:247353282-247372753:- RP11-488L18.4 Pseudogene Pseudogene GN=RP11-488L18.4 Pseudogene E_20221201_NO30_400nL_HLAc1_1e7_directIP_titration_rep2_Slot2-3_1_3513 10mio-rep3 E_20221201_NO30_400nL_HLAc1_1e7_directIP_titration_rep3_Slot2-3_1_3514.16083.375.1 1 5 1 14.21 4.33 10.11 9.88 90.0 7 0 0 11600.0 Hydrogen 31 (S) 9 YLDPAQQNL (Y) 9 LDPAQQNLY Y L/D|P/A|Q|Q\N\L 77.8 7 5 5 3 0 dual b/y bias 55.558506 1.5002 1061.524 1061.526 -0.0062 -5.8 0.5 3.8 51570.8 nuORFdb_human ENST00000421406.1_1_1:247353282-247372753:- ENST00000421406.1_1_1:247353282-247372753:- RP11-488L18.4 Pseudogene Pseudogene GN=RP11-488L18.4 Pseudogene E_20221201_NO30_400nL_HLAc1_1e7_directIP_titration_rep3_Slot2-3_1_3514 20mio-rep1 E_20221201_NO30_400nL_HLAc1_2e7_directIP_titration_rep1_Slot2-4_1_3515.16039.383.1 1 3 1 11.98 4.24 8.61 7.74 87.4 7 0 0 21400.0 Hydrogen 31 (S) 9 YLDPAQQNL (Y) 9 GRQMSQQNL Y L/D|P/A/Q|Q\N\L 77.8 7 4 5 2 0 unbiased 55.392944 1.495367 1061.5171 1061.526 -0.0062 -5.8 0.40000000000000036 3.8 51570.8 nuORFdb_human ENST00000421406.1_1_1:247353282-247372753:- ENST00000421406.1_1_1:247353282-247372753:- RP11-488L18.4 Pseudogene Pseudogene GN=RP11-488L18.4 Pseudogene E_20221201_NO30_400nL_HLAc1_2e7_directIP_titration_rep1_Slot2-4_1_3515 20mio-rep2 E_20221201_NO30_400nL_HLAc1_2e7_directIP_titration_rep3_Slot2-4_1_3518.16108.1440.2 2 5 1 15.41 4.14 13.38 11.28 87.9 7 0 0 16900.0 Hydrogen 31 (S) 9 YLDPAQQNL (Y) 9 IYGPEQVQQ Y L|D|P\A|Q|Q|N\L 77.8 7 7 5 5 0 dual b/y bias 55.636589 0.851339 531.2632 1061.526 -0.0075 -7.0 -0.7000000000000002 3.8 51570.8 nuORFdb_human ENST00000421406.1_1_1:247353282-247372753:- ENST00000421406.1_1_1:247353282-247372753:- RP11-488L18.4 Pseudogene Pseudogene GN=RP11-488L18.4 Pseudogene E_20221201_NO30_400nL_HLAc1_2e7_directIP_titration_rep3_Slot2-4_1_3518 20mio-rep3 E_20221201_NO30_400nL_HLAc1_2e7_directIP_titration_rep4_Slot2-4_1_3519.16080.367.1 1 5 1 12.3 4.29 8.95 8.01 86.8 7 0 0 21200.0 Hydrogen 31 (S) 9 YLDPAQQNL (Y) 9 LDPAQQNLY Y L/D/P/A|Q|Q\N\L 77.8 7 4 5 2 0 unbiased 55.540829 1.505122 1061.523 1061.526 -0.0062 -5.8 0.0 3.8 51570.8 nuORFdb_human ENST00000421406.1_1_1:247353282-247372753:- ENST00000421406.1_1_1:247353282-247372753:- RP11-488L18.4 Pseudogene Pseudogene GN=RP11-488L18.4 Pseudogene E_20221201_NO30_400nL_HLAc1_2e7_directIP_titration_rep4_Slot2-4_1_3519 SCP E_20221201_NO30_400nL_HLAc1_4e7_PDAC_Slot2-6_1_3524.15960.355.1 1 1 1 9.32 2.63 7.12 6.69 67.6 7 0 0 4840.0 Hydrogen 31 (S) 9 YLDPAQQNL (Y) 9 qVYNLSQNI Y L/D/P/A|Q|Q\N\L 77.8 7 4 5 2 0 unbiased 55.138279 1.511666 1061.5243 1061.526 -0.0062 -5.8 -3.4 3.8 51570.8 nuORFdb_human ENST00000421406.1_1_1:247353282-247372753:- ENST00000421406.1_1_1:247353282-247372753:- RP11-488L18.4 Pseudogene Pseudogene GN=RP11-488L18.4 Pseudogene E_20221201_NO30_400nL_HLAc1_4e7_PDAC_Slot2-6_1_3524 40mio-rep2 E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep2_Slot2-5_1_3520.16001.1427.2 2 4 1 16.48 5.78 12.12 10.7 87.3 7 0 0 84300.0 Hydrogen 31 (S) 9 YLDPAQQNL (Y) 9 IYGPEQVQQ Y L|D|P/A|Q|Q\N\L 77.8 7 6 5 4 0 dual b/y bias 55.273277 0.859709 531.2653 1061.526 -0.0035 -3.3 1.6000000000000005 3.8 51570.8 nuORFdb_human ENST00000421406.1_1_1:247353282-247372753:- ENST00000421406.1_1_1:247353282-247372753:- RP11-488L18.4 Pseudogene Pseudogene GN=RP11-488L18.4 Pseudogene E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep2_Slot2-5_1_3520 40mio-rep3 E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep3_Slot2-5_1_3521.16014.1416.2 2 5 1 16.69 4.37 12.27 12.33 80.4 7 0 0 49600.0 Hydrogen 31 (S) 9 YLDPAQQNL (Y) 9 LYDNHISSL Y L|D|P|A|Q|Q\N\L 77.8 7 7 5 5 0 dual b/y bias 55.319279 0.866504 531.2658 1061.526 -0.0015 -1.4 3.1999999999999997 3.8 51570.8 nuORFdb_human ENST00000421406.1_1_1:247353282-247372753:- ENST00000421406.1_1_1:247353282-247372753:- RP11-488L18.4 Pseudogene Pseudogene GN=RP11-488L18.4 Pseudogene E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep3_Slot2-5_1_3521 40mio-rep4 E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep4_Slot2-5_1_3522.16018.1428.2 2 5 1 14.72 2.95 11.36 11.77 86.7 7 0 0 99500.0 Hydrogen 31 (S) 9 YLDPAQQNL (Y) 9 IYGPEQVQQ Y L/D|P\A|Q|Q\N\L 77.8 7 6 4 3 0 dual b/y bias 55.318218 0.858492 531.2641 1061.526 -0.0055 -5.2 -0.5 3.8 51570.8 nuORFdb_human ENST00000421406.1_1_1:247353282-247372753:- ENST00000421406.1_1_1:247353282-247372753:- RP11-488L18.4 Pseudogene Pseudogene GN=RP11-488L18.4 Pseudogene E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep4_Slot2-5_1_3522 5mio-rep1 E_20221201_NO30_400nL_HLAc1_5e6_directIP_titration_rep1_Slot1-37_1_3502.16413.368.1 1 4 1 13.31 5.08 9.42 8.23 85.0 6 0 0 16000.0 Hydrogen 31 (S) 9 YLDPAQQNL (Y) 9 qVYNLSQNI Y L/D|P A|Q|Q\N\L 55.6 5 5 4 3 0 dual b/y bias 56.694584 1.504169 1061.5192 1061.526 -0.0062 -5.8 1.2000000000000002 3.8 51570.8 nuORFdb_human ENST00000421406.1_1_1:247353282-247372753:- ENST00000421406.1_1_1:247353282-247372753:- RP11-488L18.4 Pseudogene Pseudogene GN=RP11-488L18.4 Pseudogene E_20221201_NO30_400nL_HLAc1_5e6_directIP_titration_rep1_Slot1-37_1_3502 5mio-rep2 E_20221201_NO30_400nL_HLAc1_5e6_directIP_titration_rep2_Slot2-2_1_3510.16336.376.1 1 3 1 12.88 3.98 9.09 8.91 83.9 8 0 0 9410.0 Hydrogen 31 (S) 9 YLDPAQQNL (Y) 9 LYnPAALSnP Y/L/D/P/A|Q|Q\N\L 100.0 9 4 6 2 0 unbiased 56.423782 1.499791 1061.5215 1061.526 -0.0062 -5.8 2.8999999999999995 3.8 51570.8 nuORFdb_human ENST00000421406.1_1_1:247353282-247372753:- ENST00000421406.1_1_1:247353282-247372753:- RP11-488L18.4 Pseudogene Pseudogene GN=RP11-488L18.4 Pseudogene E_20221201_NO30_400nL_HLAc1_5e6_directIP_titration_rep2_Slot2-2_1_3510 5mio-rep3 E_20221201_NO30_400nL_HLAc1_5e6_directIP_titration_rep3_Slot2-2_1_3511.16301.1449.2 2 2 1 13.75 3.97 9.88 9.79 78.1 6 0 0 4880.0 Hydrogen 31 (S) 9 YLDPAQQNL (Y) 9 LnFSPQQVAG Y L|D|P A\Q|Q|N\L 55.6 5 6 4 4 0 dual b/y bias 56.305382 0.845883 531.2628 1061.526 -0.0075 -7.0 1.1999999999999993 3.8 51570.8 nuORFdb_human ENST00000421406.1_1_1:247353282-247372753:- ENST00000421406.1_1_1:247353282-247372753:- RP11-488L18.4 Pseudogene Pseudogene GN=RP11-488L18.4 Pseudogene E_20221201_NO30_400nL_HLAc1_5e6_directIP_titration_rep3_Slot2-2_1_3511 1mio-rep3 E_20221201_NO30_400nL_HLAc1_1e6_directIP_titration_rep4_Slot2-1_1_3509.19064.1372.2 2 1 1 7.84 2.56 6.53 5.28 62.0 7 0 0 1030.0 Hydrogen 31 (S) 10 YLDPAQQNLY (R) 10 LQPEGYQLNY Y L D\P\A\Q|Q\N\L\Y 70.0 7 7 1 1 0 b-ion bias 65.836662 0.893749 612.795 1224.59 -0.0068 -5.6 2.4000000000000004 3.8 51570.8 nuORFdb_human ENST00000421406.1_1_1:247353282-247372753:- ENST00000421406.1_1_1:247353282-247372753:- RP11-488L18.4 Pseudogene Pseudogene GN=RP11-488L18.4 Pseudogene E_20221201_NO30_400nL_HLAc1_1e6_directIP_titration_rep4_Slot2-1_1_3509 10mio-rep1 E_20221201_NO30_400nL_HLAc1_1e7_directIP_titration_rep1_Slot2-3_1_3512.18907.1364.2 2 1 1 10.78 4.24 7.6 6.54 72.5 6 0 0 5670.0 Hydrogen 31 (S) 10 YLDPAQQNLY (R) 10 IYGPEQVQQY Y L D|P A|Q|Q|N\L\Y 50.0 5 6 4 4 0 dual b/y bias 65.311211 0.898789 612.794 1224.59 -0.0088 -7.2 0.5 3.8 51570.8 nuORFdb_human ENST00000421406.1_1_1:247353282-247372753:- ENST00000421406.1_1_1:247353282-247372753:- RP11-488L18.4 Pseudogene Pseudogene GN=RP11-488L18.4 Pseudogene E_20221201_NO30_400nL_HLAc1_1e7_directIP_titration_rep1_Slot2-3_1_3512 10mio-rep2 E_20221201_NO30_400nL_HLAc1_1e7_directIP_titration_rep2_Slot2-3_1_3513.18831.1367.2 2 2 1 11.12 3.65 8.67 7.46 75.6 6 0 0 8500.0 Hydrogen 31 (S) 10 YLDPAQQNLY (R) 10 IYGPEQVQQY Y L D|P A|Q|Q|N\L\Y 50.0 5 6 4 4 0 dual b/y bias 65.046577 0.897243 612.7941 1224.59 -0.0088 -7.2 -0.5 3.8 51570.8 nuORFdb_human ENST00000421406.1_1_1:247353282-247372753:- ENST00000421406.1_1_1:247353282-247372753:- RP11-488L18.4 Pseudogene Pseudogene GN=RP11-488L18.4 Pseudogene E_20221201_NO30_400nL_HLAc1_1e7_directIP_titration_rep2_Slot2-3_1_3513 10mio-rep3 E_20221201_NO30_400nL_HLAc1_1e7_directIP_titration_rep3_Slot2-3_1_3514.18792.1360.2 2 1 1 12.79 3.67 9.11 9.12 74.8 6 0 0 7530.0 Hydrogen 31 (S) 10 YLDPAQQNLY (R) 10 LQPEGYQLNY Y L D|P A|Q|Q\N|L\Y 50.0 5 6 4 4 0 dual b/y bias 64.913673 0.901657 612.7943 1224.59 -0.0088 -7.2 -0.9000000000000004 3.8 51570.8 nuORFdb_human ENST00000421406.1_1_1:247353282-247372753:- ENST00000421406.1_1_1:247353282-247372753:- RP11-488L18.4 Pseudogene Pseudogene GN=RP11-488L18.4 Pseudogene E_20221201_NO30_400nL_HLAc1_1e7_directIP_titration_rep3_Slot2-3_1_3514 20mio-rep1 E_20221201_NO30_400nL_HLAc1_2e7_directIP_titration_rep1_Slot2-4_1_3515.18767.1362.2 2 2 1 17.24 8.04 11.44 9.21 92.2 8 0 0 26400.0 Hydrogen 31 (S) 10 YLDPAQQNLY (R) 10 IYGPEQVQQY Y L/D|P\A|Q|Q\N|L\Y 80.0 8 7 5 4 0 dual b/y bias 64.812431 0.900163 612.7921 1224.59 -0.0128 -10.5 -4.3 3.8 51570.8 nuORFdb_human ENST00000421406.1_1_1:247353282-247372753:- ENST00000421406.1_1_1:247353282-247372753:- RP11-488L18.4 Pseudogene Pseudogene GN=RP11-488L18.4 Pseudogene E_20221201_NO30_400nL_HLAc1_2e7_directIP_titration_rep1_Slot2-4_1_3515 20mio-rep2 E_20221201_NO30_400nL_HLAc1_2e7_directIP_titration_rep3_Slot2-4_1_3518.18838.1353.2 2 2 1 16.73 7.17 9.21 9.56 90.0 7 0 0 15300.0 Hydrogen 31 (S) 10 YLDPAQQNLY (R) 10 FDLDIDPNIY Y L/D|P A|Q|Q\N|L\Y 60.0 6 6 5 4 0 dual b/y bias 65.067413 0.905506 612.7935 1224.59 -0.0108 -8.8 -2.500000000000001 3.8 51570.8 nuORFdb_human ENST00000421406.1_1_1:247353282-247372753:- ENST00000421406.1_1_1:247353282-247372753:- RP11-488L18.4 Pseudogene Pseudogene GN=RP11-488L18.4 Pseudogene E_20221201_NO30_400nL_HLAc1_2e7_directIP_titration_rep3_Slot2-4_1_3518 20mio-rep3 E_20221201_NO30_400nL_HLAc1_2e7_directIP_titration_rep4_Slot2-4_1_3519.18811.1348.2 2 5 1 15.35 6.98 9.26 8.36 87.0 7 0 0 21400.0 Hydrogen 31 (S) 10 YLDPAQQNLY (R) 10 DVIDIMENLY Y L/D|P A\Q|Q\N|L\Y 60.0 6 6 4 3 0 unbiased 64.969742 0.908741 612.7946 1224.59 -0.0068 -5.6 0.20000000000000018 3.8 51570.8 nuORFdb_human ENST00000421406.1_1_1:247353282-247372753:- ENST00000421406.1_1_1:247353282-247372753:- RP11-488L18.4 Pseudogene Pseudogene GN=RP11-488L18.4 Pseudogene E_20221201_NO30_400nL_HLAc1_2e7_directIP_titration_rep4_Slot2-4_1_3519 40mio-rep1 E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep1_Slot1-04_1_3496.19003.1318.2 2 3 1 15.34 5.48 11.62 9.86 84.1 7 0 0 26600.0 Hydrogen 31 (S) 10 YLDPAQQNLY (R) 10 IYGPEQVQQY Y L D|P\A|Q|Q\N|L\Y 70.0 7 7 4 4 0 dual b/y bias 65.645645 0.927639 612.7963 1224.59 -0.0048 -3.9 -0.6999999999999997 3.8 51570.8 nuORFdb_human ENST00000421406.1_1_1:247353282-247372753:- ENST00000421406.1_1_1:247353282-247372753:- RP11-488L18.4 Pseudogene Pseudogene GN=RP11-488L18.4 Pseudogene E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep1_Slot1-04_1_3496 40mio-rep2 E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep2_Slot2-5_1_3520.18713.1332.2 2 6 1 16.41 5.9 10.9 10.51 88.1 8 0 0 58100.0 Hydrogen 31 (S) 10 YLDPAQQNLY (R) 10 LQPEGYQLNY Y L\D|P\A|Q|Q|N|L\Y 80.0 8 8 5 5 0 dual b/y bias 64.644341 0.918704 612.7957 1224.59 -0.0048 -3.9 1.0000000000000004 3.8 51570.8 nuORFdb_human ENST00000421406.1_1_1:247353282-247372753:- ENST00000421406.1_1_1:247353282-247372753:- RP11-488L18.4 Pseudogene Pseudogene GN=RP11-488L18.4 Pseudogene E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep2_Slot2-5_1_3520 40mio-rep3 E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep3_Slot2-5_1_3521.18705.1339.2 2 4 1 15.24 6.52 9.31 8.73 84.3 7 0 0 43500.0 Hydrogen 31 (S) 10 YLDPAQQNLY (R) 10 IYGPEQVQQY Y L/D|P A|Q|Q\N|L\Y 60.0 6 6 5 4 0 dual b/y bias 64.615112 0.914619 612.7965 1224.59 -0.0048 -3.9 0.6999999999999997 3.8 51570.8 nuORFdb_human ENST00000421406.1_1_1:247353282-247372753:- ENST00000421406.1_1_1:247353282-247372753:- RP11-488L18.4 Pseudogene Pseudogene GN=RP11-488L18.4 Pseudogene E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep3_Slot2-5_1_3521 40mio-rep4 E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep4_Slot2-5_1_3522.18696.1339.2 2 5 1 17.55 6.67 11.51 10.88 93.0 6 0 0 46500.0 Hydrogen 31 (S) 10 YLDPAQQNLY (R) 10 FDLDIDPNIY Y L D|P A|Q|Q|N|L\Y 50.0 5 6 5 5 0 dual b/y bias 64.56707 0.914408 612.7964 1224.59 -0.0048 -3.9 0.8000000000000003 3.8 51570.8 nuORFdb_human ENST00000421406.1_1_1:247353282-247372753:- ENST00000421406.1_1_1:247353282-247372753:- RP11-488L18.4 Pseudogene Pseudogene GN=RP11-488L18.4 Pseudogene E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep4_Slot2-5_1_3522 5mio-rep1 E_20221201_NO30_400nL_HLAc1_5e6_directIP_titration_rep1_Slot1-37_1_3502.19134.1347.2 2 1 1 9.23 2.44 7.35 6.8 62.3 7 0 0 5660.0 Hydrogen 31 (S) 10 YLDPAQQNLY (R) 10 YInKANIWnS Y L\D|P A\Q|Q\N\L\Y 60.0 6 7 2 2 0 b-ion bias 66.089165 0.909702 612.7942 1224.59 -0.0088 -7.2 -0.20000000000000018 3.8 51570.8 nuORFdb_human ENST00000421406.1_1_1:247353282-247372753:- ENST00000421406.1_1_1:247353282-247372753:- RP11-488L18.4 Pseudogene Pseudogene GN=RP11-488L18.4 Pseudogene E_20221201_NO30_400nL_HLAc1_5e6_directIP_titration_rep1_Slot1-37_1_3502 5mio-rep2 E_20221201_NO30_400nL_HLAc1_5e6_directIP_titration_rep2_Slot2-2_1_3510.19029.1370.2 2 1 1 13.96 7.19 7.26 6.77 79.2 7 0 0 2460.0 Hydrogen 31 (S) 10 YLDPAQQNLY (R) 10 DKQKDNSNLY Y L/D|P A|Q|Q\N|L\Y 60.0 6 6 5 4 0 dual b/y bias 65.724716 0.895025 612.7904 1224.59 -0.0168 -13.7 -5.0 3.8 51570.8 nuORFdb_human ENST00000421406.1_1_1:247353282-247372753:- ENST00000421406.1_1_1:247353282-247372753:- RP11-488L18.4 Pseudogene Pseudogene GN=RP11-488L18.4 Pseudogene E_20221201_NO30_400nL_HLAc1_5e6_directIP_titration_rep2_Slot2-2_1_3510 5mio-rep3 E_20221201_NO30_400nL_HLAc1_5e6_directIP_titration_rep3_Slot2-2_1_3511.19019.1365.2 2 1 1 14.82 4.33 10.68 10.49 87.4 8 0 0 2190.0 Hydrogen 31 (S) 10 YLDPAQQNLY (R) 10 IYGPEQVQQY Y L/D|P\A\Q|Q\N|L\Y 80.0 8 7 4 3 0 unbiased 65.6903 0.898322 612.7934 1224.59 -0.0108 -8.8 -0.6000000000000014 3.8 51570.8 nuORFdb_human ENST00000421406.1_1_1:247353282-247372753:- ENST00000421406.1_1_1:247353282-247372753:- RP11-488L18.4 Pseudogene Pseudogene GN=RP11-488L18.4 Pseudogene E_20221201_NO30_400nL_HLAc1_5e6_directIP_titration_rep3_Slot2-2_1_3511 SCP E_20221201_NO30_400nL_HLAc1_4e7_PDAC_Slot2-6_1_3524.27834.250.1 1 2 1 11.96 6.59 6.73 5.37 79.1 7 0 0 15600.0 Hydrogen 17 (V) 9 YLLDGQLQL (-) 9 LYVENAQLL Y L|L\D|G/Q\L\Q\L 77.8 7 6 3 2 0 b-ion bias 96.161644 1.573441 1062.5808 1062.583 -0.003 -2.8 -0.3999999999999999 3.8 2830.1 nuORFdb_human ENST00000392583.6_1_12:113435434-113435512:+ ENST00000392583.6_1_12:113435434-113435512:+ OAS2 Out-of-Frame Out-of-Frame GN=OAS2 Out-of-Frame E_20221201_NO30_400nL_HLAc1_4e7_PDAC_Slot2-6_1_3524 SCP E_20221201_NO30_400nL_HLAc1_4e7_PDAC_Slot2-6_1_3524.28265.287.1 1 1 1 9.8 4.14 5.65 5.66 78.8 6 1 1 17200.0 Hydrogen 10 (V) 8 YLMLKMSL (V) 8 qAGPAIKSML Y L|M|L|K\M\S\L 75.0 6 6 3 3 0 dual b/y bias 97.650505 1.55216 998.5394 998.541 -0.0023 -2.3 0.10000000000000009 8.59 2354.0 nuORFdb_human ENST00000402538.7_1_2:33740127-33740184:+ ENST00000402538.7_1_2:33740127-33740184:+ RASGRP3 5' uORF 5' uORF GN=RASGRP3 5' uORF E_20221201_NO30_400nL_HLAc1_4e7_PDAC_Slot2-6_1_3524 Eva-50mio-3fr AL20210827_EV_HLA_PDAC_PANFRO413_2D_50e6_W632_classI_fxn3.53485.53485.2 2 1 1 10.09 8.79 0.33 1.3 89.2 5 0 0 5210000.0 Hydrogen 24 (N) 11 YLPPIQQLPSD (L) 11 YLPPTRPGPLC Y L|P/P/I/Q Q L P-S D 27.3 3 1 4 1 1 y-ion bias 0.976 97.1 96.27 100.0 0.0 635.8349 1270.668 -0.0052 -4.1 -4.6 4.3 13186.9 nuORFdb_human ENST00000414429.5_1_6:28304569-28323149:- ENST00000414429.5_1_6:28304569-28323149:- ZSCAN31 5' Overlap uORF 5' Overlap uORF GN=ZSCAN31 5' Overlap uORF AL20210827_EV_HLA_PDAC_PANFRO413_2D_50e6_W632_classI_fxn3 SCP E_20221201_NO30_400nL_HLAc1_4e7_PDAC_Slot2-6_1_3524.28118.316.1 1 1 1 7.3 4.26 7.3 57.7 6 0 0 20900.0 Hydrogen 21 (L) 8 YLPTLTHF (S) 8 Y/L/P T/L|T|H\F 75.0 6 3 5 2 0 unbiased 97.142929 1.534683 991.521 991.525 -0.0037 -3.8 -1.4 6.74 4605.4 nuORFdb_human ENST00000537473.2_1_12:57058103-57058217:- ENST00000537473.2_1_12:57058103-57058217:- PTGES3 3' dORF 3' dORF GN=PTGES3 3' dORF E_20221201_NO30_400nL_HLAc1_4e7_PDAC_Slot2-6_1_3524 SCP E_20221201_NO30_400nL_HLAc1_4e7_PDAC_Slot2-6_1_3524.27492.1386.2 2 2 1 16.28 11.28 3.83 5.0 88.4 6 2 2 61700.0 Hydrogen 23 (I) 8 YLRVQLKI (-) 8 IQVRYLKI Y/L/R|V Q|L\K\I 75.0 6 4 4 2 0 unbiased 94.979988 0.88503 516.8309 1032.656 -0.0017 -1.6 0.7999999999999998 9.99 3590.4 nuORFdb_human ENST00000443024.6_1_9:95050481-95051592:- ENST00000443024.6_1_9:95050481-95051592:- IARS Out-of-Frame Out-of-Frame GN=IARS Out-of-Frame E_20221201_NO30_400nL_HLAc1_4e7_PDAC_Slot2-6_1_3524 20mio-rep3 E_20221201_NO30_400nL_HLAc1_2e7_directIP_titration_rep4_Slot2-4_1_3519.21340.1326.2 2 2 1 11.62 10.46 5.16 1.15 77.6 7 0 1 8370.0 Hydrogen 24 (R) 10 YLYTLVISEK (E) 10 YLYTLVITDK Y/L|Y|T|L/V I|S\E K 60.0 6 5 6 4 0 dual b/y bias 73.70385 0.922739 614.8441 1228.682 -0.0016 -1.3 4.5 6.0 5267.1 nuORFdb_human ENST00000502376.1_1_3:98243929-98244067:- ENST00000502376.1_1_3:98243929-98244067:- RPL38P4 Pseudogene Pseudogene GN=RPL38P4 Pseudogene E_20221201_NO30_400nL_HLAc1_2e7_directIP_titration_rep4_Slot2-4_1_3519 40mio-rep4 E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep4_Slot2-5_1_3522.21330.1324.2 2 1 1 10.52 9.42 4.48 1.1 74.1 7 0 1 3160.0 Hydrogen 24 (R) 10 YLYTLVISEK (E) 10 YLYTLVITDK Y L|Y|T|L/V/I/S\E K 60.0 6 4 6 3 0 unbiased 73.666733 0.923936 614.8409 1228.682 -0.0076 -6.2 -1.5 6.0 5267.1 nuORFdb_human ENST00000502376.1_1_3:98243929-98244067:- ENST00000502376.1_1_3:98243929-98244067:- RPL38P4 Pseudogene Pseudogene GN=RPL38P4 Pseudogene E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep4_Slot2-5_1_3522 SCP E_20221201_NO30_400nL_HLAc1_4e7_PDAC_Slot2-6_1_3524.11518.1400.2 2 1 1 16.17 8.99 10.21 7.18 91.4 7 1 1 5940.0 Hydrogen 28 (V) 8 YMVVSRHL (-) 8 MYVVSLRH Y/M|V|V/S/R|H\L 100.0 8 4 6 3 0 dual b/y bias 39.792065 0.876503 502.7696 1004.535 -0.0019 -1.9 0.5 8.75 4203.0 nuORFdb_human ENST00000481195.5_1_5:133536728-133537609:- ENST00000481195.5_1_5:133536728-133537609:- PPP2CA Out-of-Frame Out-of-Frame GN=PPP2CA Out-of-Frame E_20221201_NO30_400nL_HLAc1_4e7_PDAC_Slot2-6_1_3524 SCP E_20221201_NO30_400nL_HLAc1_4e7_PDAC_Slot2-6_1_3524.8724.1326.2 2 1 1 14.33 4.47 9.39 9.86 74.0 7 1 1 5760.0 M23m Hydrogen 15 (F) 9 YPECEIRTm (G) 9 YPmTmQGRAQ Y/P|E|C|E\I/R\T m 77.8 7 5 5 3 0 dual b/y bias Carbamidomethylation C:Carbamidomethylation,m:Oxidized methionine 30.139132 0.922886 607.7608 1198.523 15.9916 -2.7 -0.30000000000000027 4.53 8680.8 nuORFdb_human ENST00000423834.6_1_7:72469123-72469612:- ENST00000423834.6_1_7:72469123-72469612:-|ENST00000569650.1_1_7:72469123-72470067:-|ENST00000380775.3_7:74298994-74299868:-|ENST00000429726.2_7:74299379-74299868:-|ENST00000359898.5_7:74299379-74304447:- STAG3L3 Pseudogene Pseudogene GN=STAG3L3 Pseudogene E_20221201_NO30_400nL_HLAc1_4e7_PDAC_Slot2-6_1_3524 Exploris_FAIMS VH20221207_MKP_FAIMS2022E_HLAc1_4e7_PDAC.22788.22788.2 2 1 1 9.79 3.88 8.8 5.92 66.8 8 1 1 2410000.0 M23m Hydrogen 15 (F) 9 YPECEIRTm (G) 9 YPEEKLDcV Y/P|E/C/E/I/R/T/m 100.0 9 1 8 1 0 y-ion bias Carbamidomethylation C:Carbamidomethylation,m:Oxidized methionine 0.873 100.0 47.35 120.0 -70.0 607.7605 1198.523 15.9906 -3.5 -0.8999999999999999 4.53 8680.8 nuORFdb_human ENST00000423834.6_1_7:72469123-72469612:- ENST00000423834.6_1_7:72469123-72469612:-|ENST00000569650.1_1_7:72469123-72470067:-|ENST00000380775.3_7:74298994-74299868:-|ENST00000429726.2_7:74299379-74299868:-|ENST00000359898.5_7:74299379-74304447:- STAG3L3 Pseudogene Pseudogene GN=STAG3L3 Pseudogene VH20221207_MKP_FAIMS2022E_HLAc1_4e7_PDAC Eva-50mio-3fr AL20210827_EV_HLA_PDAC_PANFRO413_2D_50e6_W632_classI_fxn1.38330.38330.1 1 1 1 16.28 5.62 4.14 10.66 95.8 8 0 0 961000.0 Hydrogen 28 (N) 9 YPEGLGEVL (Y) 9 YGPEGLELV Y/P\E\G\L\G\E\V\L 100.0 9 7 1 0 0 b-ion bias 0.973 56.4 68.8 100.0 0.0 976.4978 976.499 -0.0008 -0.8 -0.9 3.8 6164.8 nuORFdb_human ENST00000347770.8_1_8:103870273-103876088:- ENST00000347770.8_1_8:103870273-103876088:- AZIN1 5' uORF 5' uORF GN=AZIN1 5' uORF AL20210827_EV_HLA_PDAC_PANFRO413_2D_50e6_W632_classI_fxn1 SCP E_20221201_NO30_400nL_HLAc1_4e7_PDAC_Slot2-6_1_3524.23167.430.1 1 2 1 9.33 1.62 3.24 7.7 71.6 6 0 0 17000.0 Hydrogen 28 (N) 9 YPEGLGEVL (Y) 9 YLPEnQVL Y/P E|G L\G\E\V\L 55.6 5 5 2 1 0 b-ion bias 80.036507 1.46783 976.4994 976.499 0.0004 0.4 2.8 3.8 6164.8 nuORFdb_human ENST00000347770.8_1_8:103870273-103876088:- ENST00000347770.8_1_8:103870273-103876088:- AZIN1 5' uORF 5' uORF GN=AZIN1 5' uORF E_20221201_NO30_400nL_HLAc1_4e7_PDAC_Slot2-6_1_3524 Eva-50mio-3fr AL20210827_EV_HLA_PDAC_PANFRO413_2D_50e6_W632_classI_fxn1.40379.40488.2 2 2 2 13.89 5.71 7.93 8.18 77.5 8 0 0 44600000.0 Hydrogen 28 (N) 10 YPEGLGEVLY (R) 10 SNSTGAEVVLY Y P\E\G-L\G\E\V|L/Y 80.0 8 6 2 1 1 b-ion bias 0.999 97.1 72.67 100.0 0.0 570.2847 1139.562 0.0002 0.2 0.1 3.79 6164.8 nuORFdb_human ENST00000347770.8_1_8:103870273-103876088:- ENST00000347770.8_1_8:103870273-103876088:- AZIN1 5' uORF 5' uORF GN=AZIN1 5' uORF AL20210827_EV_HLA_PDAC_PANFRO413_2D_50e6_W632_classI_fxn1 Exploris AL20221201_MKP_PDAC_01.52952.53072.2 2 1 1 12.06 4.66 6.23 7.39 72.1 8 0 0 282000000.0 Hydrogen 28 (N) 10 YPEGLGEVLY (R) 10 EQTGATASVIY Y P\E\G-L\G\E\V|L/Y 80.0 8 6 2 1 1 b-ion bias 0.994 91.5 80.77 7.44 0.0 570.2849 1139.562 0.0007 0.6 -0.20000000000000007 3.79 6164.8 nuORFdb_human ENST00000347770.8_1_8:103870273-103876088:- ENST00000347770.8_1_8:103870273-103876088:- AZIN1 5' uORF 5' uORF GN=AZIN1 5' uORF AL20221201_MKP_PDAC_01 SCP E_20221201_NO30_400nL_HLAc1_4e7_PDAC_Slot2-6_1_3524.24414.238.1 1 3 1 16.42 8.52 12.07 7.9 93.7 9 0 0 48200.0 Hydrogen 28 (N) 10 YPEGLGEVLY (R) 10 SNSTGAEVVLY Y/P/E|G\L\G\E\V\L\Y 100.0 10 7 3 1 0 b-ion bias 84.343483 1.580839 1139.5587 1139.562 -0.0019 -1.7 0.7 3.79 6164.8 nuORFdb_human ENST00000347770.8_1_8:103870273-103876088:- ENST00000347770.8_1_8:103870273-103876088:- AZIN1 5' uORF 5' uORF GN=AZIN1 5' uORF E_20221201_NO30_400nL_HLAc1_4e7_PDAC_Slot2-6_1_3524 Exploris_FAIMS VH20221207_MKP_FAIMS2022E_HLAc1_4e7_PDAC.58800.58944.2 2 2 1 14.73 5.99 8.53 8.73 78.1 8 0 0 86700000.0 Hydrogen 28 (N) 10 YPEGLGEVLY (R) 10 SNSTGAEVVLY Y P\E\G\L\G\E\V|L/Y 80.0 8 7 2 1 0 b-ion bias 0.999 98.5 102.43 25.25 -70.0 570.2834 1139.562 -0.0024 -2.1 0.5 3.79 6164.8 nuORFdb_human ENST00000347770.8_1_8:103870273-103876088:- ENST00000347770.8_1_8:103870273-103876088:- AZIN1 5' uORF 5' uORF GN=AZIN1 5' uORF VH20221207_MKP_FAIMS2022E_HLAc1_4e7_PDAC Exploris AL20221201_MKP_PDAC_01.31923.31923.2 2 1 1 7.86 7.86 7.86 65.4 7 1 1 218000000.0 Hydrogen 64 (M) 9 YPVDSVKVM (W) 9 Y/P|V/D\S V/K/V/M 77.8 7 2 6 1 0 y-ion bias 0.898 88.7 50.67 87.72 0.0 519.2719 1037.534 0.003 2.8 1.9999999999999998 5.83 8846.7 nuORFdb_human ENST00000519192.1_1_8:23386515-23389239:+ ENST00000519192.1_1_8:23386515-23389239:+ SLC25A37 ncRNA Processed Transcript lncRNA GN=SLC25A37 ncRNA Processed Transcript AL20221201_MKP_PDAC_01 SCP E_20221201_NO30_400nL_HLAc1_4e7_PDAC_Slot2-6_1_3524.14929.1448.2 2 2 1 12.15 2.3 9.98 9.85 67.2 7 1 1 139000.0 Hydrogen 64 (M) 9 YPVDSVKVM (W) 9 YPSTTIPKM Y/P/V|D|S V|K\V\M 77.8 7 5 5 3 0 dual b/y bias 51.576622 0.846033 519.2689 1037.534 -0.0029 -2.8 -0.3999999999999999 5.83 8846.7 nuORFdb_human ENST00000519192.1_1_8:23386515-23389239:+ ENST00000519192.1_1_8:23386515-23389239:+ SLC25A37 ncRNA Processed Transcript lncRNA GN=SLC25A37 ncRNA Processed Transcript E_20221201_NO30_400nL_HLAc1_4e7_PDAC_Slot2-6_1_3524 Exploris_FAIMS VH20221207_MKP_FAIMS2022E_HLAc1_4e7_PDAC.37802.37802.2 2 2 1 12.16 3.04 11.55 9.12 85.1 8 1 1 145000000.0 Hydrogen 64 (M) 9 YPVDSVKVM (W) 9 MYPVDSVKV Y/P|V/D-S-V/K/V/M 100.0 9 1 6 1 2 y-ion bias 0.994 73.3 69.85 15.6 -70.0 519.269 1037.534 -0.0029 -2.8 -0.19999999999999973 5.83 8846.7 nuORFdb_human ENST00000519192.1_1_8:23386515-23389239:+ ENST00000519192.1_1_8:23386515-23389239:+ SLC25A37 ncRNA Processed Transcript lncRNA GN=SLC25A37 ncRNA Processed Transcript VH20221207_MKP_FAIMS2022E_HLAc1_4e7_PDAC Eva-50mio-3fr AL20210827_EV_HLA_PDAC_PANFRO413_2D_50e6_W632_classI_fxn1.17405.17405.2 2 2 1 14.04 8.03 6.78 6.02 86.3 8 1 1 15300000.0 M72m Hydrogen 64 (M) 9 YPVDSVKVm (W) 9 YPVDLGDKF Y/P|V/D/S/V/K/V/m 100.0 9 1 8 1 0 y-ion bias m:Oxidized methionine 0.873 79.3 33.07 100.0 0.0 527.2676 1037.534 15.9943 -0.6 -0.7 5.83 8846.7 nuORFdb_human ENST00000519192.1_1_8:23386515-23389239:+ ENST00000519192.1_1_8:23386515-23389239:+ SLC25A37 ncRNA Processed Transcript lncRNA GN=SLC25A37 ncRNA Processed Transcript AL20210827_EV_HLA_PDAC_PANFRO413_2D_50e6_W632_classI_fxn1 SCP E_20221201_NO30_400nL_HLAc1_4e7_PDAC_Slot2-6_1_3524.11069.1418.2 2 1 1 13.1 8.04 7.04 5.06 71.3 6 1 1 15300.0 M72m Hydrogen 64 (M) 9 YPVDSVKVm (W) 9 YPVLGmSLSA Y/P|V|D/S V|K V\m 55.6 5 4 5 3 0 dual b/y bias m:Oxidized methionine 38.241322 0.865307 527.2645 1037.534 15.9891 -5.5 -3.1 5.83 8846.7 nuORFdb_human ENST00000519192.1_1_8:23386515-23389239:+ ENST00000519192.1_1_8:23386515-23389239:+ SLC25A37 ncRNA Processed Transcript lncRNA GN=SLC25A37 ncRNA Processed Transcript E_20221201_NO30_400nL_HLAc1_4e7_PDAC_Slot2-6_1_3524 SCP E_20221201_NO30_400nL_HLAc1_4e7_PDAC_Slot2-6_1_3524.25437.1415.2 2 14 1 14.51 8.18 10.46 6.33 86.5 7 0 0 176000.0 Hydrogen 3 (N) 8 YQGHFHLF (-) 8 YQnYTVIF Y/Q|G\H|F|H\L\F 100.0 8 6 4 3 0 dual b/y bias 87.876839 0.866954 524.752 1048.5 -0.0032 -3.1 -0.7000000000000002 6.92 1275.4 nuORFdb_human ENST00000283632.4_1_2:86968058-86968091:+ ENST00000283632.4_1_2:86968058-86968091:+ RMND5A Out-of-Frame Out-of-Frame GN=RMND5A Out-of-Frame E_20221201_NO30_400nL_HLAc1_4e7_PDAC_Slot2-6_1_3524 10mio-rep2 E_20221201_NO30_400nL_HLAc1_1e7_directIP_titration_rep2_Slot2-3_1_3513.14685.425.1 1 1 1 10.31 3.68 6.19 6.63 71.3 6 0 0 2190.0 Hydrogen 20 (Y) 9 YSETTLTSY (F) 9 TETSLTYSY Y S E\T|T\L|T\S\Y 66.7 6 6 2 2 0 b-ion bias 50.729736 1.470519 1064.4651 1064.478 -0.0082 -7.7 -1.0 4.0 4809.6 nuORFdb_human ENST00000501011.6_1_11:82784390-82784510:+ ENST00000501011.6_1_11:82784390-82784510:+|ENST00000526795.1_1_11:82784390-82788120:+|TCONS_00019410_11:82784390-82802303:+ RAB30-AS1 lincRNA lncRNA GN=RAB30-AS1 lincRNA E_20221201_NO30_400nL_HLAc1_1e7_directIP_titration_rep2_Slot2-3_1_3513 20mio-rep1 E_20221201_NO30_400nL_HLAc1_2e7_directIP_titration_rep1_Slot2-4_1_3515.14618.426.1 1 1 1 8.02 2.43 5.7 5.59 67.8 7 0 0 2480.0 Hydrogen 20 (Y) 9 YSETTLTSY (F) 9 TETSLTYSY Y S/E/T\T\L\T\S\Y 77.8 7 5 2 0 0 b-ion bias 50.487431 1.4701 1064.4674 1064.478 -0.0082 -7.7 -1.5 4.0 4809.6 nuORFdb_human ENST00000501011.6_1_11:82784390-82784510:+ ENST00000501011.6_1_11:82784390-82784510:+|ENST00000526795.1_1_11:82784390-82788120:+|TCONS_00019410_11:82784390-82802303:+ RAB30-AS1 lincRNA lncRNA GN=RAB30-AS1 lincRNA E_20221201_NO30_400nL_HLAc1_2e7_directIP_titration_rep1_Slot2-4_1_3515 20mio-rep2 E_20221201_NO30_400nL_HLAc1_2e7_directIP_titration_rep3_Slot2-4_1_3518.14730.423.1 1 1 1 8.4 2.06 6.91 6.34 70.3 6 0 0 2060.0 Hydrogen 20 (Y) 9 YSETTLTSY (F) 9 TETSLTYSY Y S E/T|T\L\T\S\Y 66.7 6 5 2 1 0 b-ion bias 50.878857 1.471935 1064.4715 1064.478 -0.0082 -7.7 -1.4000000000000004 4.0 4809.6 nuORFdb_human ENST00000501011.6_1_11:82784390-82784510:+ ENST00000501011.6_1_11:82784390-82784510:+|ENST00000526795.1_1_11:82784390-82788120:+|TCONS_00019410_11:82784390-82802303:+ RAB30-AS1 lincRNA lncRNA GN=RAB30-AS1 lincRNA E_20221201_NO30_400nL_HLAc1_2e7_directIP_titration_rep3_Slot2-4_1_3518 40mio-rep1 E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep1_Slot1-04_1_3496.14908.394.1 1 1 1 7.54 0.71 6.04 6.83 69.7 6 0 0 16100.0 Hydrogen 20 (Y) 9 YSETTLTSY (F) 9 SIPTnTMHY Y S E/T\T\L\T\S\Y 66.7 6 5 1 0 0 b-ion bias 51.496769 1.489032 1064.4744 1064.478 -0.0082 -7.7 -4.5 4.0 4809.6 nuORFdb_human ENST00000501011.6_1_11:82784390-82784510:+ ENST00000501011.6_1_11:82784390-82784510:+|ENST00000526795.1_1_11:82784390-82788120:+|TCONS_00019410_11:82784390-82802303:+ RAB30-AS1 lincRNA lncRNA GN=RAB30-AS1 lincRNA E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep1_Slot1-04_1_3496 5mio-rep2 E_20221201_NO30_400nL_HLAc1_5e6_directIP_titration_rep2_Slot2-2_1_3510.14895.432.1 1 1 1 8.41 1.8 6.0 6.61 66.2 6 0 0 1520.0 Hydrogen 20 (Y) 9 YSETTLTSY (F) 9 MHTLcLLD Y S E/T\T|L\T|S\Y 66.7 6 5 3 2 0 unbiased 51.445934 1.466433 1064.471 1064.478 -0.0082 -7.7 0.9999999999999991 4.0 4809.6 nuORFdb_human ENST00000501011.6_1_11:82784390-82784510:+ ENST00000501011.6_1_11:82784390-82784510:+|ENST00000526795.1_1_11:82784390-82788120:+|TCONS_00019410_11:82784390-82802303:+ RAB30-AS1 lincRNA lncRNA GN=RAB30-AS1 lincRNA E_20221201_NO30_400nL_HLAc1_5e6_directIP_titration_rep2_Slot2-2_1_3510 5mio-rep3 E_20221201_NO30_400nL_HLAc1_5e6_directIP_titration_rep3_Slot2-2_1_3511.14917.434.1 1 2 1 8.31 2.09 5.69 6.22 63.0 7 0 0 3230.0 Hydrogen 20 (Y) 9 YSETTLTSY (F) 9 EGSGGGTLHSY Y S/E/T/T|L\T\S\Y 77.8 7 4 4 1 0 unbiased 51.527309 1.465121 1064.4707 1064.478 -0.0082 -7.7 0.4999999999999991 4.0 4809.6 nuORFdb_human ENST00000501011.6_1_11:82784390-82784510:+ ENST00000501011.6_1_11:82784390-82784510:+|ENST00000526795.1_1_11:82784390-82788120:+|TCONS_00019410_11:82784390-82802303:+ RAB30-AS1 lincRNA lncRNA GN=RAB30-AS1 lincRNA E_20221201_NO30_400nL_HLAc1_5e6_directIP_titration_rep3_Slot2-2_1_3511 SCP E_20221201_NO30_400nL_HLAc1_4e7_PDAC_Slot2-6_1_3524.9256.1364.2 2 1 1 11.15 8.7 11.15 61.1 7 1 1 15400.0 Hydrogen 8 (I) 8 YSNHEYKW (-) 8 Y/S/N/H|E|Y|K\W 100.0 8 4 6 3 0 dual b/y bias 31.976311 0.898755 563.7511 1126.495 -0.0005 -0.5 1.9 6.75 1925.1 nuORFdb_human ENST00000371804.3_1_10:91152438-91162049:+ ENST00000371804.3_1_10:91152438-91162049:+ IFIT1 5' Overlap uORF 5' Overlap uORF GN=IFIT1 5' Overlap uORF E_20221201_NO30_400nL_HLAc1_4e7_PDAC_Slot2-6_1_3524 20mio-rep1 E_20221201_NO30_400nL_HLAc1_2e7_directIP_titration_rep1_Slot2-4_1_3515.26808.1434.2 2 2 1 12.73 5.17 7.56 7.56 85.1 5 0 0 5080.0 Hydrogen 9 (K) 9 YSSEIWDLY (F) 9 CFSQRSDLY Y S S E|I/W|D|L\Y 55.6 5 4 4 3 0 dual b/y bias 92.572441 0.854886 588.2616 1175.525 -0.0088 -7.5 -1.2999999999999998 3.67 3215.6 nuORFdb_human ENST00000345122.7_1_14:32559748-32559826:+ ENST00000345122.7_1_14:32559748-32559826:+ ARHGAP5 5' uORF 5' uORF GN=ARHGAP5 5' uORF E_20221201_NO30_400nL_HLAc1_2e7_directIP_titration_rep1_Slot2-4_1_3515 20mio-rep2 E_20221201_NO30_400nL_HLAc1_2e7_directIP_titration_rep3_Slot2-4_1_3518.26827.1429.2 2 4 1 15.49 6.21 9.98 9.28 92.5 5 0 0 7170.0 Hydrogen 9 (K) 9 YSSEIWDLY (F) 9 LTnHPnSnIY Y S S E|I/W|D|L\Y 55.6 5 4 4 3 0 dual b/y bias 92.657593 0.858048 588.2641 1175.525 -0.0048 -4.1 2.2 3.67 3215.6 nuORFdb_human ENST00000345122.7_1_14:32559748-32559826:+ ENST00000345122.7_1_14:32559748-32559826:+ ARHGAP5 5' uORF 5' uORF GN=ARHGAP5 5' uORF E_20221201_NO30_400nL_HLAc1_2e7_directIP_titration_rep3_Slot2-4_1_3518 20mio-rep3 E_20221201_NO30_400nL_HLAc1_2e7_directIP_titration_rep4_Slot2-4_1_3519.26857.1425.2 2 3 1 11.94 8.12 6.03 3.81 82.9 5 0 0 6730.0 Hydrogen 9 (K) 9 YSSEIWDLY (F) 9 CPSGGHVADIY Y S S E\I/W|D|L\Y 55.6 5 4 3 2 0 unbiased 92.757004 0.860649 588.2632 1175.525 -0.0068 -5.8 0.0 3.67 3215.6 nuORFdb_human ENST00000345122.7_1_14:32559748-32559826:+ ENST00000345122.7_1_14:32559748-32559826:+ ARHGAP5 5' uORF 5' uORF GN=ARHGAP5 5' uORF E_20221201_NO30_400nL_HLAc1_2e7_directIP_titration_rep4_Slot2-4_1_3519 40mio-rep1 E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep1_Slot1-04_1_3496.27050.216.1 1 3 1 16.15 4.12 11.86 12.04 94.6 5 0 0 12300.0 Hydrogen 9 (K) 9 YSSEIWDLY (F) 9 SEYSIWYEV Y S S E|I|W\D\L\Y 55.6 5 5 2 2 0 b-ion bias 93.450577 1.593598 1175.5182 1175.525 -0.0055 -4.7 -1.5 3.67 3215.6 nuORFdb_human ENST00000345122.7_1_14:32559748-32559826:+ ENST00000345122.7_1_14:32559748-32559826:+ ARHGAP5 5' uORF 5' uORF GN=ARHGAP5 5' uORF E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep1_Slot1-04_1_3496 40mio-rep2 E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep2_Slot2-5_1_3520.26825.228.1 1 3 1 14.27 4.54 9.83 9.73 90.7 5 0 0 14400.0 Hydrogen 9 (K) 9 YSSEIWDLY (F) 9 SEYSIWYEV Y S S E|I|W\D\L\Y 55.6 5 5 2 2 0 b-ion bias 92.668571 1.5868 1175.5242 1175.525 -0.0055 -4.7 0.20000000000000018 3.67 3215.6 nuORFdb_human ENST00000345122.7_1_14:32559748-32559826:+ ENST00000345122.7_1_14:32559748-32559826:+ ARHGAP5 5' uORF 5' uORF GN=ARHGAP5 5' uORF E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep2_Slot2-5_1_3520 40mio-rep3 E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep3_Slot2-5_1_3521.26833.1424.2 2 4 1 15.39 5.61 9.17 9.77 91.3 5 0 0 18200.0 Hydrogen 9 (K) 9 YSSEIWDLY (F) 9 CFSQRSDLY Y S S E|I/W|D|L\Y 55.6 5 4 4 3 0 dual b/y bias 92.689133 0.861244 588.2639 1175.525 -0.0048 -4.1 0.5 3.67 3215.6 nuORFdb_human ENST00000345122.7_1_14:32559748-32559826:+ ENST00000345122.7_1_14:32559748-32559826:+ ARHGAP5 5' uORF 5' uORF GN=ARHGAP5 5' uORF E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep3_Slot2-5_1_3521 40mio-rep4 E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep4_Slot2-5_1_3522.26843.1423.2 2 4 1 14.3 6.03 8.84 8.26 87.2 5 0 0 17900.0 Hydrogen 9 (K) 9 YSSEIWDLY (F) 9 CPSGGHVADIY Y S S E|I/W|D|L\Y 55.6 5 4 4 3 0 dual b/y bias 92.716339 0.862248 588.2637 1175.525 -0.0048 -4.1 0.6000000000000005 3.67 3215.6 nuORFdb_human ENST00000345122.7_1_14:32559748-32559826:+ ENST00000345122.7_1_14:32559748-32559826:+ ARHGAP5 5' uORF 5' uORF GN=ARHGAP5 5' uORF E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep4_Slot2-5_1_3522 40mio-rep1 E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep1_Slot1-04_1_3496.7126.1446.2 2 1 1 13.2 5.21 4.15 7.99 77.7 7 1 2 7760.0 Hydrogen 357 (T) 8 YSSQIRKL (R) 8 SYSGLAKRL Y/S|S/Q|I/R\K\L 100.0 8 4 5 2 0 unbiased 24.622911 0.847227 497.7857 994.568 -0.0033 -3.3 -0.09999999999999964 9.99 51147.2 nuORFdb_human ENST00000469130.5_1_1:201751782-201763782:+ ENST00000469130.5_1_1:201751782-201763782:+|ENST00000477118.2_3_1:201756811-201759996:+ NAV1 3' Overlap dORF 3' Overlap dORF GN=NAV1 3' Overlap dORF E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep1_Slot1-04_1_3496 40mio-rep2 E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep2_Slot2-5_1_3520.6864.1468.2 2 1 1 8.81 4.35 1.38 4.45 59.9 6 1 2 5680.0 Hydrogen 357 (T) 8 YSSQIRKL (R) 8 YSANSVLKI Y/S/S/Q/I R\K\L 75.0 6 2 4 0 0 y-ion bias 23.718029 0.833655 497.784 994.568 -0.0073 -7.3 -2.3999999999999995 9.99 51147.2 nuORFdb_human ENST00000469130.5_1_1:201751782-201763782:+ ENST00000469130.5_1_1:201751782-201763782:+|ENST00000477118.2_3_1:201756811-201759996:+ NAV1 3' Overlap dORF 3' Overlap dORF GN=NAV1 3' Overlap dORF E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep2_Slot2-5_1_3520 40mio-rep4 E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep4_Slot2-5_1_3522.6893.1466.2 2 2 1 8.84 4.65 3.58 4.2 64.1 7 1 2 11800.0 Hydrogen 357 (T) 8 YSSQIRKL (R) 8 YSLNEKKL Y/S/S/Q|I\R\K\L 100.0 8 4 4 1 0 unbiased 23.8102 0.83503 497.7838 994.568 -0.0073 -7.3 -2.5999999999999996 9.99 51147.2 nuORFdb_human ENST00000469130.5_1_1:201751782-201763782:+ ENST00000469130.5_1_1:201751782-201763782:+|ENST00000477118.2_3_1:201756811-201759996:+ NAV1 3' Overlap dORF 3' Overlap dORF GN=NAV1 3' Overlap dORF E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep4_Slot2-5_1_3522 40mio-rep4 E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep4_Slot2-5_1_3522.3490.1409.2 2 1 1 9.59 3.53 5.82 6.06 61.8 7 1 2 8590.0 Hydrogen 24 (Y) 9 YSSSRKVTY (H) 9 VESELLHTY Y/S/S/S/R/K V|T\Y 77.8 7 2 6 1 0 y-ion bias 12.059387 0.870783 545.7772 1090.553 -0.006 -5.5 -0.7999999999999998 9.7 5535.6 nuORFdb_human ENST00000478691.5_1_1:23664326-23665089:- ENST00000478691.5_1_1:23664326-23665089:-|ENST00000453465.1_1_10:21915067-21915193:-|ENST00000490652.1_1_1:23664981-23665089:- HNRNPR Out-of-Frame Out-of-Frame GN=HNRNPR Out-of-Frame E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep4_Slot2-5_1_3522 20mio-rep1 E_20221201_NO30_400nL_HLAc1_2e7_directIP_titration_rep1_Slot2-4_1_3515.18657.1438.2 2 1 1 8.72 3.19 4.7 5.53 60.2 6 1 1 5080.0 Hydrogen 76 (D) 9 YSTSRLITF (F) 9 IHVVDSGLTF Y S|T/S R\L\I|T\F 55.6 5 5 3 2 0 unbiased 64.432762 0.85234 544.2911 1087.578 -0.0035 -3.2 3.0 8.75 11616.1 nuORFdb_human ENST00000605920.1_1_10:81586793-81587114:+ ENST00000605920.1_1_10:81586793-81587114:+|ENST00000439559.2_1_10:89102840-89103146:+|ENST00000609350.1_1_2:128644229-128644535:+ RP11-182L21.6 lincRNA lncRNA GN=RP11-182L21.6 lincRNA E_20221201_NO30_400nL_HLAc1_2e7_directIP_titration_rep1_Slot2-4_1_3515 20mio-rep3 E_20221201_NO30_400nL_HLAc1_2e7_directIP_titration_rep4_Slot2-4_1_3519.18723.1433.2 2 1 1 7.94 3.54 3.45 4.4 67.3 6 1 1 6110.0 Hydrogen 76 (D) 9 YSTSRLITF (F) 9 TKnKKGYTF Y S/T/S R\L\I|T\F 55.6 5 4 3 1 0 unbiased 64.666016 0.855914 544.2902 1087.578 -0.0055 -5.1 0.7000000000000002 8.75 11616.1 nuORFdb_human ENST00000605920.1_1_10:81586793-81587114:+ ENST00000605920.1_1_10:81586793-81587114:+|ENST00000439559.2_1_10:89102840-89103146:+|ENST00000609350.1_1_2:128644229-128644535:+ RP11-182L21.6 lincRNA lncRNA GN=RP11-182L21.6 lincRNA E_20221201_NO30_400nL_HLAc1_2e7_directIP_titration_rep4_Slot2-4_1_3519 40mio-rep1 E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep1_Slot1-04_1_3496.18841.1391.2 2 3 1 14.91 7.76 7.16 7.16 79.8 7 1 1 20300.0 Hydrogen 76 (D) 9 YSTSRLITF (F) 9 TSKYnILTF Y/S|T/S R|L|I|T\F 77.8 7 5 6 4 0 dual b/y bias 65.086327 0.881751 544.2922 1087.578 -0.0015 -1.4 1.8000000000000003 8.75 11616.1 nuORFdb_human ENST00000605920.1_1_10:81586793-81587114:+ ENST00000605920.1_1_10:81586793-81587114:+|ENST00000439559.2_1_10:89102840-89103146:+|ENST00000609350.1_1_2:128644229-128644535:+ RP11-182L21.6 lincRNA lncRNA GN=RP11-182L21.6 lincRNA E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep1_Slot1-04_1_3496 20mio-rep1 E_20221201_NO30_400nL_HLAc1_2e7_directIP_titration_rep1_Slot2-4_1_3515.13264.1277.2 2 1 1 12.1 8.69 6.21 3.4 72.7 5 0 0 2930.0 Hydrogen 33 (Y) 11 YTEHEYTSLGY (T) 11 nnnVTQSYSIGY Y T E H E-Y|T|S|L|G\Y 54.5 6 5 4 4 1 dual b/y bias 45.813194 0.952936 681.7943 1362.585 -0.0041 -3.0 3.2 4.51 7536.3 nuORFdb_human ENST00000476493.5_1_1:33099602-33116090:- ENST00000476493.5_1_1:33099602-33116090:- ZBTB8OS 3' dORF 3' dORF GN=ZBTB8OS 3' dORF E_20221201_NO30_400nL_HLAc1_2e7_directIP_titration_rep1_Slot2-4_1_3515 40mio-rep1 E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep1_Slot1-04_1_3496.13567.1250.2 2 1 1 14.11 7.73 4.81 6.37 82.4 6 0 0 9390.0 Hydrogen 33 (Y) 11 YTEHEYTSLGY (T) 11 nnnVTQSYSIGY Y T E H\E Y|T|S|L|G\Y 45.5 5 6 4 4 0 dual b/y bias 46.864864 0.969965 681.7929 1362.585 -0.0061 -4.5 -1.2999999999999998 4.51 7536.3 nuORFdb_human ENST00000476493.5_1_1:33099602-33116090:- ENST00000476493.5_1_1:33099602-33116090:- ZBTB8OS 3' dORF 3' dORF GN=ZBTB8OS 3' dORF E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep1_Slot1-04_1_3496 40mio-rep2 E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep2_Slot2-5_1_3520.13255.1270.2 2 1 1 11.8 9.35 4.31 2.45 76.4 6 0 0 3970.0 Hydrogen 33 (Y) 11 YTEHEYTSLGY (T) 11 nnnVTQSYSIGY Y T E H\E Y\T|S|L|G\Y 45.5 5 6 3 3 0 b-ion bias 45.789089 0.957491 681.7937 1362.585 -0.0041 -3.0 1.9000000000000004 4.51 7536.3 nuORFdb_human ENST00000476493.5_1_1:33099602-33116090:- ENST00000476493.5_1_1:33099602-33116090:- ZBTB8OS 3' dORF 3' dORF GN=ZBTB8OS 3' dORF E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep2_Slot2-5_1_3520 SCP E_20221201_NO30_400nL_HLAc1_4e7_PDAC_Slot2-6_1_3524.16447.1364.2 2 1 1 13.44 6.3 7.71 7.14 79.0 7 1 1 33600.0 Hydrogen 172 (L) 8 YTFIKHEF (C) 8 YTHIQYLF Y/T|F/I/K/H\E\F 100.0 8 3 5 1 0 unbiased 56.819843 0.898866 542.7758 1084.546 -0.0015 -1.4 1.0 6.75 21094.8 nuORFdb_human ENST00000512410.1_1_4:175416602-175443578:- ENST00000512410.1_1_4:175416602-175443578:- HPGD 3' Overlap dORF 3' Overlap dORF GN=HPGD 3' Overlap dORF E_20221201_NO30_400nL_HLAc1_4e7_PDAC_Slot2-6_1_3524 40mio-rep4 E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep4_Slot2-5_1_3522.8524.1417.2 2 1 1 10.2 5.19 5.57 5.01 73.9 6 2 2 6630.0 Hydrogen 13 (M) 8 YTSRAKLW (V) 8 GKGSTIYLW Y/T/S/R A/K|L\W 75.0 6 2 5 1 0 y-ion bias 29.441559 0.865639 512.7806 1024.557 -0.0027 -2.7 2.0 9.99 3478.2 nuORFdb_human ENST00000488494.5_1_10:33221437-33221518:- ENST00000488494.5_1_10:33221437-33221518:-|ENST00000423113.5_1_10:33218934-33221518:- ITGB1 Out-of-Frame Out-of-Frame GN=ITGB1 Out-of-Frame E_20221201_NO30_400nL_HLAc1_4e7_directIP_titration_rep4_Slot2-5_1_3522 Exploris_FAIMS VH20221207_MKP_FAIMS2022E_HLAc1_4e7_PDAC.22311.22311.3 3 1 1 8.32 2.41 8.32 5.9 67.0 6 1 2 1900000.0 Hydrogen 25 (G) 9 YVNEKRYPL (-) 9 YVASVSVVRAM Y/V/N/E/K/R Y/P L 55.6 5 0 6 0 0 y-ion bias 0.969 100.0 46.5 120.0 -70.0 394.5479 1181.631 -0.0022 -1.9 0.7000000000000002 8.5 3632.2 nuORFdb_human ENST00000360031.6_2_20:25176439-25187192:+ ENST00000360031.6_2_20:25176439-25187192:+|ENST00000435520.5_2_20:25177840-25187192:+ ENTPD6 5' Overlap uORF 5' Overlap uORF GN=ENTPD6 5' Overlap uORF VH20221207_MKP_FAIMS2022E_HLAc1_4e7_PDAC SCP E_20221201_NO30_400nL_HLAc1_4e7_PDAC_Slot2-6_1_3524.15867.1285.2 2 2 1 12.49 5.19 5.97 7.31 79.0 6 1 1 14300.0 Hydrogen 5 (L) 9 YYCKINVVY (I) 9 ERPGESWVVY Y Y/C K\I\N\V|V\Y 55.6 5 5 2 1 0 b-ion bias Carbamidomethylation C:Carbamidomethylation, 54.816399 0.948231 611.3006 1221.597 -0.0025 -2.1 0.2999999999999998 8.43 3079.7 nuORFdb_human ENST00000367660.3_1_6:139364011-139364086:+ ENST00000367660.3_1_6:139364011-139364086:+ ABRACL 3' dORF 3' dORF GN=ABRACL 3' dORF E_20221201_NO30_400nL_HLAc1_4e7_PDAC_Slot2-6_1_3524