Parameter Value Version 1.5.4.0 User name gordon Machine name DESKTOP-RQFRNPM Date of writing 02/23/2023 11:12:13 Fixed modifications Carbamidomethyl (C) Include contaminants True PSM FDR 0.01 Protein FDR 0.01 Site FDR 0.01 Use Normalized Ratios For Occupancy True Min. peptide Length 7 Min. score for unmodified peptides 0 Min. score for modified peptides 40 Min. delta score for unmodified peptides 0 Min. delta score for modified peptides 6 Min. unique peptides 0 Min. razor peptides 1 Min. peptides 1 Use only unmodified peptides and True Modifications included in protein quantification Oxidation (M);Acetyl (Protein N-term) Peptides used for protein quantification Razor Discard unmodified counterpart peptides True Min. ratio count 2 Use delta score False iBAQ False iBAQ log fit False Match between runs False Find dependent peptides False Fasta file D:\tap_the_pap\uniprot_homo_sapien_UP000005640_release_2022_05.fasta;D:\tap_the_pap\uniprot_mus_muscula_UP000000589_release_2022_05.fasta;D:\tap_the_pap\uniprot_rattus_norvegicus_UP000002494_release_2022_05.fasta Decoy mode revert Include contaminants True Advanced ratios True Fixed andromeda index folder Temporary folder Combined folder location D:\tap_the_pap\900_pooled_lavages_wk1-7_2023FEB23 Second peptides True Stabilize large LFQ ratios True Separate LFQ in parameter groups False Require MS/MS for LFQ comparisons True Calculate peak properties False Main search max. combinations 200 Advanced site intensities True Write msScans table True Write msmsScans table True Write ms3Scans table True Write allPeptides table True Write mzRange table True Top x mass window [Da] 100 Max. peptide mass [Da] 30000 Min. peptide length for unspecific search 8 Max. peptide length for unspecific search 25 Razor protein FDR True Disable MD5 False Max mods in site table 3 Match unidentified features False MS/MS tol. (FTMS) 20 ppm Top MS/MS peaks per 100 Da. (FTMS) 12 MS/MS deisotoping (FTMS) True MS/MS deisotoping tolerance (FTMS) 7 MS/MS deisotoping tolerance unit (FTMS) ppm MS/MS higher charges (FTMS) True MS/MS water loss (FTMS) True MS/MS ammonia loss (FTMS) True MS/MS dependent losses (FTMS) True MS/MS recalibration (FTMS) False MS/MS tol. (ITMS) 0.5 Da Top MS/MS peaks per 100 Da. (ITMS) 8 MS/MS deisotoping (ITMS) False MS/MS deisotoping tolerance (ITMS) 0.15 MS/MS deisotoping tolerance unit (ITMS) Da MS/MS higher charges (ITMS) True MS/MS water loss (ITMS) True MS/MS ammonia loss (ITMS) True MS/MS dependent losses (ITMS) True MS/MS recalibration (ITMS) False MS/MS tol. (TOF) 40 ppm Top MS/MS peaks per 100 Da. (TOF) 10 MS/MS deisotoping (TOF) True MS/MS deisotoping tolerance (TOF) 0.01 MS/MS deisotoping tolerance unit (TOF) Da MS/MS higher charges (TOF) True MS/MS water loss (TOF) True MS/MS ammonia loss (TOF) True MS/MS dependent losses (TOF) True MS/MS recalibration (TOF) False MS/MS tol. (Unknown) 0.5 Da Top MS/MS peaks per 100 Da. (Unknown) 8 MS/MS deisotoping (Unknown) False MS/MS deisotoping tolerance (Unknown) 0.15 MS/MS deisotoping tolerance unit (Unknown) Da MS/MS higher charges (Unknown) True MS/MS water loss (Unknown) True MS/MS ammonia loss (Unknown) True MS/MS dependent losses (Unknown) True MS/MS recalibration (Unknown) False Site tables Oxidation (M)Sites.txt