Raw file Experiment Enzyme Enzyme mode Enzyme first search Enzyme mode first search Use enzyme first search Variable modifications Multi modifications Variable modifications first search Use variable modifications first search Requantify Multiplicity Max. missed cleavages Labels0 LC-MS run type Time-dependent recalibration MS MS/MS MS3 MS/MS Submitted MS/MS Submitted (SIL) MS/MS Submitted (ISO) MS/MS Submitted (PEAK) MS/MS Identified MS/MS Identified (SIL) MS/MS Identified (ISO) MS/MS Identified (PEAK) MS/MS Identified [%] MS/MS Identified (SIL) [%] MS/MS Identified (ISO) [%] MS/MS Identified (PEAK) [%] Peptide Sequences Identified Peaks Peaks Sequenced Peaks Sequenced [%] Peaks Repeatedly Sequenced Peaks Repeatedly Sequenced [%] Isotope Patterns Isotope Patterns Sequenced Isotope Patterns Sequenced (z>1) Isotope Patterns Sequenced [%] Isotope Patterns Sequenced (z>1) [%] Isotope Patterns Repeatedly Sequenced Isotope Patterns Repeatedly Sequenced [%] Recalibrated Av. Absolute Mass Deviation [ppm] Mass Standard Deviation [ppm] Av. Absolute Mass Deviation [mDa] Mass Standard Deviation [mDa] Label free norm param wk1_s_trap_1 1_s_trap_1 LysC Specific False Oxidation (M);Acetyl (Protein N-term) False False 1 2 Standard 16757 9383 0 13041 5725 0 7316 1158 1100 0 58 8.88 19.21 NaN 0.79 184 1004568 6300 0.63 1406 22.32 32740 2874 2597 8.78 15.32 1283 44.64 + 0.51231 0.81712 0.35734 0.60903 wk1_s_trap_2 1_s_trap_2 LysC Specific False Oxidation (M);Acetyl (Protein N-term) False False 1 2 Standard 16876 9131 0 12267 5995 0 6272 1166 1126 0 40 9.51 18.78 NaN 0.64 189 1041946 5973 0.57 1505 25.2 37131 2915 2614 7.85 13.97 1374 47.14 + 0.53784 0.81777 0.37314 0.59602 wk3_s_trap_1 3_s_trap_1 LysC Specific False Oxidation (M);Acetyl (Protein N-term) False False 1 2 Standard 16940 8084 0 11777 4391 0 7386 707 684 0 23 6 15.58 NaN 0.31 144 1086420 5544 0.51 1066 19.23 38362 2255 1868 5.88 14.14 1006 44.61 + 0.50643 0.82864 0.38375 0.68778 wk3_s_trap_2 3_s_trap_2 LysC Specific False Oxidation (M);Acetyl (Protein N-term) False False 1 2 Standard 17032 7990 0 11285 4695 0 6590 746 734 0 12 6.61 15.63 NaN 0.18 142 1108216 5442 0.49 1139 20.93 41810 2389 2024 5.71 14.09 1061 44.41 + 0.58509 0.87227 0.42241 0.67712 wk5_s_trap_1 5_s_trap_1 LysC Specific False Oxidation (M);Acetyl (Protein N-term) False False 1 2 Standard 17390 6696 0 9494 3898 0 5596 556 540 0 16 5.86 13.85 NaN 0.29 127 991203 4667 0.47 1006 21.56 26509 2026 1788 7.64 12.6 944 46.59 + 0.48646 0.75824 0.34508 0.56372 wk5_s_trap_2 5_s_trap_2 LysC Specific False Oxidation (M);Acetyl (Protein N-term) False False 1 2 Standard 17457 6522 0 8854 4190 0 4664 571 559 0 12 6.45 13.34 NaN 0.26 123 1021681 4464 0.44 1078 24.15 29016 2154 1921 7.42 12.45 997 46.29 + 0.43452 0.64685 0.31526 0.49446 wk7_s_trap_1 7_s_trap_1 LysC Specific False Oxidation (M);Acetyl (Protein N-term) False False 1 2 Standard 17753 5825 0 7224 4426 0 2798 1236 1179 0 57 17.11 26.64 NaN 2.04 141 1049481 3359 0.32 1034 30.78 39435 1937 1790 4.91 9.24 904 46.67 + 0.4637 0.73465 0.31798 0.5345 wk7_s_trap_2 7_s_trap_2 LysC Specific False Oxidation (M);Acetyl (Protein N-term) False False 1 2 Standard 17879 5412 0 6559 4265 0 2294 1219 1169 0 50 18.59 27.41 NaN 2.18 142 1083998 2948 0.27 1031 34.97 43075 1804 1634 4.19 7.79 887 49.17 + 0.45856 0.75177 0.31134 0.54768 wk5_digest_1 5_digest_1 LysC Specific False Oxidation (M);Acetyl (Protein N-term) False False 1 2 Standard 19124 970 0 1585 355 0 1230 5 5 0 0 0.32 1.41 NaN 0 3 911137 713 0.08 121 16.97 20956 236 127 1.13 2.91 77 32.63 + 0.58924 0.79576 0.31977 0.43843 wk5_digest_2 5_digest_2 LysC Specific False Oxidation (M);Acetyl (Protein N-term) False False 1 2 Standard 19188 773 0 1235 311 0 924 5 5 0 0 0.4 1.61 NaN 0 3 914640 564 0.06 105 18.62 21131 191 98 0.9 2.43 67 35.08 + 2.4553 2.7501 2.5871 3.6314 wk7_digest_1 7_digest_1 LysC Specific False Oxidation (M);Acetyl (Protein N-term) False False 1 2 Standard 19270 457 0 694 220 0 474 0 0 0 0 0 0 NaN 0 0 925200 335 0.04 69 20.6 21264 137 52 0.64 1.5 53 38.69 + wk7_digest_2 7_digest_2 LysC Specific False Oxidation (M);Acetyl (Protein N-term) False False 1 2 Standard 19296 322 0 472 172 0 300 0 0 0 0 0 0 NaN 0 0 923722 242 0.03 50 20.66 21473 111 46 0.52 1.35 40 36.04 + 1_s_trap_1 184 1_s_trap_2 189 3_s_trap_1 144 3_s_trap_2 142 5_digest_1 3 5_digest_2 3 5_s_trap_1 127 5_s_trap_2 123 7_digest_1 0 7_digest_2 0 7_s_trap_1 141 7_s_trap_2 142 Total 214962 61565 0 84487 38643 0 45844 7369 7101 0 268 8.72 18.38 NaN 0.58 250 12062212 372902 19029 16559 5.1 11.15 8693 45.68 0.50485 0.79424 0.35858 0.61381