Animalia Hymenoptera Braconidae Fernandez-TrianaJose L.ShimboriEduardo M.WhitfieldJames B.Penteado-DiasAngelica M.ShawScott R.BoudreaultCarolineSonesJaymePerezKateBrownAllisonManjunathRamyaBurnsJohn M.HebertP. D. N.SmithM. AlexHallwachsWinnifredJanzenDaniel H.A revision of the parasitoid wasp genus Alphomelon Mason with the description of 30 new species (Hymenoptera, Braconidae)Zookeys160820231175516210.3897/zookeys.1175.105068 E5D30FB0-8520-5C00-8026-DD50E38F82C4 Alphomelon yanayacu https://zoobank.org/E1745F15-F7D9-435B-8C9B-F172F9B4A53D Fernandez-Triana & Shimborisp. nov.Fig. 90A–EType material.

Holotype. Ecuador • Female, CNC; Guayas, Yanayacu, Las Penas, 2°10'55.74"S, 79°52'31.98"W, 300 m; 29–30.VIII.1977; Voucher code: CNC1065901.

Paratype. Ecuador • Female, CNC. Voucher code: CNC1196598.

Distribution.

Ecuador.

Biology.

No data.

DNA barcoding.

Not available.

Etymology.

Named after the holotype locality.

Diagnostic description.

White patch on gena: extending to occiput but not to clypeus. Tegula/humeral complex color: yellow/yellow. Mesonotum color: mostly dark brown to black. Metasoma color: mostly black or dark brown. Tarsal claws spines: 3. Pterostigma shape: comparatively less elongate, its length ≤ 2.5× its central height and usually more rounded with at least one of its lower margins curved. T1 sculpture: strongly sculptured on at least apical half or more. T1 central ridge: clearly marked by two raised carinae. T2 weakly sculptured along margins. Ovipositor sheaths length: longer than first segment of metatarsus. Body length: 3.6–3.7 mm. Fore wing length: 3.6–3.8 mm.

10.3897/zookeys.1175.105068.figure90077C8797-06F8-571F-9C1F-9873F6BDE74B

Alphomelonyanayacu Fernandez-Triana & Shimbori holotype female CNC1065901 A habitus, lateral B head, frontal C fore wing D propodeum and metasoma, dorsal E mesosoma, dorsal.

https://binary.pensoft.net/fig/89319210.3897/zookeys.1175.105068.figure91F31D3A63-A6FA-5702-B26A-D9A14369D139

Parasite cocoons AAlphomelonadrianguadamuzi 06-SRNP-34573 DHJ436594 BAlphomelonandydeansi 15-SRNP-32133 DHJ729026.

https://binary.pensoft.net/fig/89319310.3897/zookeys.1175.105068.figure9246220D08-3C94-5EE1-90F8-D9E6A0DB639C

Parasite cocoons AAlphomelonarecaphile 16-SRNP-70716-DHJ732874 BAlphomelonbromeliphile 06-SRNP-60114-DHJ419211.

https://binary.pensoft.net/fig/89319410.3897/zookeys.1175.105068.figure93BD78E353-36EC-5353-BE03-FC6D0679A0AF

Parasite cocoons AAlphomeloncalixtomoragai 08-SRNP-23629-DHJ448941 BAlphomeloncarolinacanoae 06-SRNP-45140-DHJ414816.

https://binary.pensoft.net/fig/89319510.3897/zookeys.1175.105068.figure94F5FF5F22-EDCC-5E61-AFCA-F409DC041882

Parasite cocoons AAlphomelonchristerhanssoni 16-SRNP-65052-DHJ488258 BAlphomelondiniamartinezae 15-SRNP-30195-DHJ803603.

https://binary.pensoft.net/fig/89319610.3897/zookeys.1175.105068.figure95B2485C2F-B53F-5000-AD35-7BA4DB0B8DCD

Parasite cocoons AAlphomelonduvalierbricenoi 05-SRNP-24943-DHJ415451 BAlphomeloneldaarayae 05-SRNP-40812-DHJ436395.

https://binary.pensoft.net/fig/89319710.3897/zookeys.1175.105068.figure96923964C7-D7D9-555A-A7BD-27E2E8A9EC0A

Parasite cocoons AAlphomelongloriasihezarae 09-SRNP-45000-DHJ465227 BAlphomelonguillermopereirai 16-SRNP-70422-DHJ732639.

https://binary.pensoft.net/fig/89319810.3897/zookeys.1175.105068.figure97C7DA3125-013C-5F72-AF95-E2F99B325AF0

Parasite cocoons AAlphomelonhazelcambroneroae 17-SRNP-27155-DHJ731343 BAlphomelonjosecortesi 15-SRNP-32024-DHJ728940.

https://binary.pensoft.net/fig/89319910.3897/zookeys.1175.105068.figure983775BD29-BCAA-5719-9641-CD9E00BFC117

Parasite cocoons and caterpillars AAlphomelonkeineraragoni 15-SRNP-71451-DHJ727801 BAlphomelonluciarosae 12-SRNP-65952-DHJ487984.

https://binary.pensoft.net/fig/89320010.3897/zookeys.1175.105068.figure99A15DD993-6919-5E8E-80C4-2E7ECC295204

Parasite cocoons and caterpillars AAlphomelonmanuelriosi 15-SRNP-31843-DHJ728686 BAlphomelonjosecortesi 15-SRNP-2670-DHJ704019.

https://binary.pensoft.net/fig/89320110.3897/zookeys.1175.105068.figure10042BF4479-F39E-5E1B-864E-14135B70229C

Parasite cocoons and caterpillars AAlphomelonmikesharkeyi 09-SRNP-72362-DHJ474249 BAlphomelonnanosoma 15-SRNP-31923-DHJ728702.

https://binary.pensoft.net/fig/89320210.3897/zookeys.1175.105068.figure1019A2583E3-3D52-53D8-B6E5-0D7E6074C61D

Parasite cocoons AAlphomelonosvaldoespinozai 08-SRNP-72533-DHJ447339 BAlphomelonpetronariosae 15-SRNP-71861-DHJ727939.

https://binary.pensoft.net/fig/89320310.3897/zookeys.1175.105068.figure102DEA42DF3-8266-5548-BA9C-1D6C551FD3D7

Parasite cocoons AAlphomelonricardocaleroi 15-SRNP-55879-DHJ492398 BAlphomelonsergioriosi 08-SRNP-32245-DHJ462411.

https://binary.pensoft.net/fig/89320410.3897/zookeys.1175.105068.figure103B6E899CE-2D04-5B19-8B9D-2A3927E9E76A

Parasite cocoons and caterpillars AAlphomelontalidicida 17-SRNP-30054-DHJ735829 BAlphomelonxestopyga 16-SRNP-55580-DHJ492508.

https://binary.pensoft.net/fig/89320510.3897/zookeys.1175.105068.figure104EC06C78E-6BBD-525A-9554-134B1ADDB531

Maximum Likelihood analysis of all species of Alphomelon with full or almost full (> 500 base pairs) DNA barcodes. The tree was constructed based on the General Time Reversible model (Nei and Kumar 2000), made using MEGA X (Kumar et al. 2018). Tip labels include the accession for the sequence from the type series used to construct the tree.

https://binary.pensoft.net/fig/89320610.3897/zookeys.1175.105068.figure1057E6E9432-C3A5-5F9A-9252-FE26D20C5FD6

Diagnostic base pairs to differentiate species that fall within the same BIN. Red rectangles show two diagnostic base pairs (Single Nucleotide Polymorphisms) in specimens of Alphomelonguillermopereirai, A.melanoscelis, and A.paramelanoscelis within BINBOLD:AAB8584. Numbers were derived by aligning sequences to the cytochrome c oxidase subunit I (COI) region of the full mitochondrial reference genome of Drosophilamelanogaster (NC_024511.2).

https://binary.pensoft.net/fig/893207NeiMKumarS (2000) Molecular Evolution and Phylogenetics.Oxford University Press, New York, 350 pp.KumarSStecherGLiMKnyazCTamuraK (2018) MEGA X: Molecular Evolutionary Genetics Analysis across computing platforms.Molecular Biology and Evolution35(6): 1547–1549. https://doi.org/10.1093/molbev/msy096