Macdonald et al. 10.1073/pnas.0409849102.

Supporting Information

Files in this Data Supplement:

Supporting Figure 5
Supporting Figure 6
Supporting Figure 7
Supporting Table 3




Fig. 5. LacZ expression in newborn OGH+/– animals. Whole-mount analysis of P0 OGH+/–. (a) Skull from ventral soft palate. Images 1 and 2 show enlarged image from a. (b) Skull lateral view of salivary glands. Image 3 shows enlarged image from b.





Fig. 6. Food intake in WT and OGH-Tg mice on a standard diet. Food intake was measured by using the Columbus metabolic cage system, and it represents food intake in a 24-hour period.





Fig. 7. Respiratory quotient in WT and OGH-Tg mice on a standard or high-fat diet. Respiratory quotient was measured by using the Columbus metabolic cage system over a 72-hour period on 8- to 10-week-old male or female mice maintained on a standard chow diet (A and B) or after 6 weeks of exposure to a high-fat diet (C and D). Respiratory quotient was calculated from the ratio of VCO2/VO2.





 

Table 3. Serum measures in WT and OGH-Tg mice on a high-fat diet

Measurement

Male WT

Male OGH-Tg

Female WT

Female Ogh-Tg

Glucose, mg/dl

263 ± 8

226 ± 10*

219 ± 15

175 ± 4*

Triglycerides, mg/dl

65 ± 2

53 ± 5*

96 ± 17

71 ± 7

Cholesterol, mg/dl

185 ± 12

110 ± 14*

113 ± 12

71 ± 12*

Insulin, ng/ml

1.49 ± 0.3

0.29 ± 0.06*

0.70 ± 0.20

0.41 ± 0.24

Groups of male and female mice were fasted overnight and killed on the next morning for serum collection. Glucose, triglycerides, cholesterol, and insulin were measured in male and female mice maintained on a high-fat diet for 12 and 6 weeks, respectively. *, P < 0.05, compared with WT littermate controls (one-way ANOVA).