This file is the output result file of MoFi annotation of intact Myozyme glycoforms. In the Rows 1-107 the parameters used for intact glycoform annotation in MoFi are reported: Row 4 Myozyme sequence Row 5 experimental (deconvoluted) average masses associated to the pekas of Myozyme deconvoluted mass spectrum Row 7 to 15 average atomic mass taken as reported by IUPAC used for masses calculation Row 17 to 25 atomic composition of the monosaccharide Hex (Hexose), HexNAc (N-acetylhexosamine), Neu5Ac (N-acetylneuramic acid), Fuc (Fucose) and of the modification Phospho (phosphoryl group), Cysteinyl, Acetyl and pyro-Glu (pyroglutamate at the N-terminus of the protein). Row 26 to 104 Glycan lists used for intact glycoform annotation. glycan name, monosaccharide and modification composition, site of occupancy and relative abundances are reported. From row 105:results of MoFi search Column name Description ID identity number of the annotation of the mass peak Exp. Mass experimental (deconvoluted) average mass % peak height relative to the most intense peak Hit all annotations that agree in their glycan set, but differ in site occupancy Hit Score hit score as calculated by MoFi, indicates glycoform contribution to total peak height, totals 100 for each peak #Perms Number of permutations per hit Theo. Mass theoretical mass of the annotation Da deviation of experimental and theoretical mass in Dalton ppm deviation of experimental and theoretical mass in parts per million Hex number of hexose (hex) residues in the glycan annotation HexNAc number of N-acetylhexosamine (HexNAc) residues in the glycan annotation Neu5Ac number of sialic acid (Neu5Gc) residues in the glycan annotation Fuc number of fucose (Fuc) residues in the glycan annotation Phospho number of phophoryl groups in the glycan annotation Cysteinyl number of cysteinylation (fixed at 1) Acetyl number of acetyl groups in the glycan annotation pyro-Glu number of N-terminal pyro-glutamate (fixed at 1) Perm identity number of the permutation Perm Score permutation score as calculated by MoFi, indicates glycoform contribution to the hit, totals hit score of the related hit N177 N-glycan at Asn177 N334 N-glycan at Asn334 N414 N-glycan at Asn414 N596 N-glycan at Asn596 N826 N-glycan at Asn826 N84 N-glycan at Asn84 N869 N-glycan at Asn869