Table 2. Overlap of overrepresented and underrepresented oligonucleotides in human SH,M versus Sh,m, and RESCUE-annotated exonic splicing enhancers (ESEs)
|
Cutoff for enrichment |
3.84 |
5.02 |
6.64 |
|
Fraction of oligonucleotides enriched in SH,M exons that overlap RESCUE-annotated ESE hexamers |
0.36 |
0.39 |
0.39 |
|
Fraction of oligonucleotides enriched in Sh,m exons that overlap RESCUE-annotated ESE hexamers |
0.88 |
0.90 |
0.83 |
|
P value |
0 |
1.0E–13 |
1.0E–6 |
Oligonucleotides (4 or 5 mers) that were enriched in SH,M or Sh,m at different cutoffs (3.84, 5.02, 6.64, corresponding to χ2 values for different P value cutoffs) were considered to overlap a RESCUE-annotated ESE if a subsequence of length 4 or 5 bases of an oligonucleotide was an exact match to a continuous subsequence constructed from a RESCUE-annotated ESE hexamer (1). RESCUE-annotated ESEs are underrepresented in the set of k-mers that are enriched in SH,M, as opposed to the set that is enriched in Sh,m. The statistical significance was assessed by using the χ2 test with Yates correction factor.
1. Fairbrother, W. G., Yeh, R. F., Sharp, P. A. & Burge, C. B. (2002) Science 297, 1007–1013.