Primer

Primer Sequence (5'–3')

PCR1 5'

gatcACTAGTGAATCCACAGAAACTAGC

PCR1 3'

atcgcccg GGT TCA CGA TTC TTc AcT CCT GGG CAA TGT AGG

PCR2l 5'

CATGCTCGAGacatgttgaagaatatg

PCR2l 3'

GGTTCACGATTCTTTATTCGCGTTTGCGTTTCACAGC

PCR2r 5'

cGAATAAAGAATCGTGAACCTGTTGCATGTTATGTTTCAGCGTGT

PCR2r 3'

acgtaagcttgggccCGTGTGCCTGTGTTGTGC

PCRi 5'

aaacaattccttcctggacccag

PCRi 3'

CCGCGGttCaCtcttgggcaatgtatgaaaaagtg

PCRii 5'

CCGCGGggtctcaaagatcttattccc

PCRii 3'

TTAATTAACTCGAGtctgcaacaacatgaagatag

PCRiii 5'

TTAATTAAAGCGCTGGCCGGCCAGCGGCCGCccaagaataaagaatcgtttgtg

PCRiii 3'

TCTAGACACCGGTGcacgtgtgcctgtgttgtgc

PCRA 5'

agtctgggccggtccggccaccctgggcggtctcaaag

PCRA 3'

gtgtcatatggatacacgggg

PCRB 5'

acggatattaatactagtgacctcgtcctctgagcc

PCRB 3'

gtgctacatatgggccggaccggccccggagtcgcgggcaaagc

5' Hexon SA

ACGTCCGCGGcgccgccagcggtccgcgatcg

3' Hexon SA

TCGACTCGAGcttggaaagcgggcgcgcgg

5' SV40T(SacII)

ACGTCCGCGGccacttgtgtgggttgattgc

3' SV40 T

TCGACTCGAGCctaaaatacacaaacaattag.

5' SV40²⁰⁰ (Xho)

GATCGACTCGAGccacttgtgtgggttgattgc

5' SV40³⁴⁶ (Xho)

GATCGACTCGAGCgtatttgcttcttccttaaatcc

MB1

aacggaatccgcgcccaccg

MB2

TGGCCGGCCAGCGCTTTAATTAACTCGAGCctgcaacaacatgaagatagtgg

MB3

TAATTAAAGCGCTGGCCGGCCAGCGGCCGCAagcatcacttacttaaaatcag

MB4

tggaggaccggtttccgtgtc

MUT1

cgagctctcgaggccaccatggcgg

MUT2

cagcaggcgctcggaactggcgtggtagaagatgtttgttctggagacatattcatcc

MUT3

aacatcttctaccacgccagttccgagcgcctgctgactgtggggcaccctttttatg

MUT4

cccatgttaaccctgctgtctgttc

L1 5' Eco47III

ggccttactggatccAGCGCTcgcagggcaagaataatgggattgc

L1 3' poly(A)/NotI

GCTTGCGGCCGCCACAAGTAATCAATGACACAtttatttgcgtttgcgtttcacagc