Data set identification

Source of RNA or method of isolation

Subject identification*

GEO accession no.

Notes

Data set no. 1 – Human Reference cRNA Standard

Stratagene Human Reference RNA Standard taken to a single cRNA target

Replicate-1

GSM42742

The product of a single cRNA synthesis from Human Reference RNA standard was hybridized to four different U133A chips.

Replicate -2

GSM42775

Replicate -3

GSM42814

Replicate -4

GSM42803

Data set no. 2 – Human Reference RNA

Stratagene Human Reference RNA Standard

First Hybridization

GSM42816

A single Human Reference RNA standard underwent four independent cRNA synthesis reactions and was hybridized to four different U133 A chips.

Second Hybridization

GSM42817

Third Hybridization

GSM42818

Fourth Hyhbridization

GSM42819

Data set no. 3 – Effect of RNA Extraction on Gene Expression

Total Leukocyte Isolation of Buffy Coat Fraction

Healthy Control Subject-1

GSM42788

Blood leukocyte RNA was isolated from six healthy subjects using three different isolation techniques in the same subject

Healthy Control Subject-2

GSM42777

Healthy Control Subject-3

GSM42745

Healthy Control Subject-4

GSM42804

Healthy Control Subject-5

GSM42790

Healthy Control Subject-6

GSM42763

Total Leukocyte Isolation of Lysed Whole Blood

Healthy Control Subject-1

GSM42771

Healthy Control Subject-2

GSM42799

Healthy Control Subject-3

GSM42784

Healthy Control Subject-4

GSM42778

Healthy Control Subject-5

GSM42772

Healthy Control Subject-6

GSM42751

PAXGene™

Healthy Control Subject-1

GSM42774

Healthy Control Subject-2

GSM42770

Healthy Control Subject-3

GSM42752

Healthy Control Subject-4

GSM42747

Healthy Control Subject-5

GSM42743

Healthy Control Subject-6

GSM42809

Data set no. 4 – Effect of Tissue Preservation Method on Muscle Gene Expression Patterns

70% Ethanol

Burned Subject -1

GSM42739

Waste muscle specimens were obtained from three subjects with extensive burn injuries, and were processed by three different techniques: snap freezing in liquid nitrogen, immersion in 70% ethanol or in RNAlater™

Burned Subject -2

GSM42736

Burned Subject -3

GSM42733

RNAlater™

Burned Subject -1

GSM42740

Burned Subject -2

GSM42737

Burned Subject -3

GSM42732

Snap-frozen

Burned Subject -1

GSM42738

Burned Subject -2

GSM42734

Burned Subject -3

GSM42735

Data set #5 – Variance in Gene Expression Over 24 Hours in the Same Healthy Subject

Total Leukocyte Isolation from Lysed Whole Blood

Healthy Control Subject-1 Time = 0 hrs

GSM42805

Blood was collected from four healthy subjects over a 24 hour period, and RNA isolated from a lysed whole blood preparation.

Healthy Control Subject-1 Time = 2 hrs

GSM42760

Healthy Control Subject-1 Time = 4 hrs

GSM42772

Healthy Control Subject-1 Time = 6 hrs

GSM42753

Healthy Control Subject-1 Time = 9 hrs

GSM42806

Healthy Control Subject-1 Time = 24 hrs

GSM42762

Healthy Control Subject-2 Time = 0 hrs

GSM42794

Healthy Control Subject-2 Time = 2 hrs

GSM42756

Healthy Control Subject-2 Time = 4 hrs

GSM42751

Healthy Control Subject-2 Time = 24 hrs

GSM42779

Healthy Control Subject-3 Time = 0 hrs

GSM42764

Healthy Control Subject-3 Time = 2 hrs

GSM42811

Healthy Control Subject-3 Time = 4 hrs

GSM42797

Healthy Control Subject-3 Time = 6 hrs

GSM42768

Healthy Control Subject-3 Time = 9 hrs

GSM42746

Healthy Control Subject-3 Time = 24 hrs

GSM42757

Healthy Control Subject-4 Time = 0 hrs

GSM42767

Healthy Control Subject-4 Time = 2 hrs

GSM42812

Healthy Control Subject-4 Time = 4 hrs

GSM42795

Healthy Control Subject-4 Time = 6 hrs

GSM42783

Healthy Control Subject-4 Time = 9 hrs

GSM42761

Healthy Control Subject-4 Time = 24 hrs

GSM42741

Data set no. 6 – Variance in Gene Expression Among Different Healthy Subjects

Total Leukocyte Isolation from Lysed Whole Blood

Healthy Control Subject-1

GSM42771

Blood was collected from 17 different healthy subjects, and RNA isolated from a lysed whole blood preparation.

Healthy Control Subject-2

GSM42799

Healthy Control Subject-3

GSM42784

Healthy Control Subject-4

GSM42778

Healthy Control Subject-5

GSM42772

Healthy Control Subject-6

GSM42751

Healthy Control Subject-7

GSM42783

Healthy Control Subject-8

GSM42764

Healthy Control Subject-9

GSM42802

Healthy Control Subject-10

GSM42792

Healthy Control Subject-11

GSM42807

Healthy Control Subject-12

GSM42801

Healthy Control Subject-13

GSM42759

Healthy Control Subject-14

GSM42798

Healthy Control Subject-15

GSM42780

Healthy Control Subject-16

GSM42813

Healthy Control Subject-17

GSM42750

Data set no. 7 – Variance in Gene Expression Among Different Trauma Patients

Total Leukocyte Isolation from Lysed Whole Blood

Trauma Subject-1

GSM42776

Blood was collected from 14 different severely traumatized subjects, and RNA isolated from a lysed whole blood preparation.

Trauma Subject-2

GSM42808

Trauma Subject-3

GSM42754

Trauma Subject-4

GSM42787

Trauma Subject-5

GSM42749

Trauma Subject-6

GSM42755

Trauma Subject-7

GSM42796

Trauma Subject-8

GSM42815

Trauma Subject-9

GSM42810

Trauma Subject-10

GSM42744

Trauma Subject-11

GSM42785

Trauma Subject-12

GSM42765

Trauma Subject-13

GSM42789

Trauma Subject-14

GSM42800

Data set no. 8 – Gene Expression in Enriched Leukocyte Populations from Healthy Control Subjects

Total Leukocyte Isolation from Lysed Whole Blood

Healthy Control Subject-1

GSM42802

Blood was collected from five healthy control subjects, and RNA was isolated in the same subject from total leukocytes, enriched T-cells and enriched monocytes

Healthy Control Subject-2

GSM42792

Healthy Control Subject-3

GSM42807

Healthy Control Subject-4

GSM42801

Healthy Control Subject-5

GSM42759

Enriched T-cell Population

Healthy Control Subject-1

GSM42786

Healthy Control Subject-2

GSM42773

Healthy Control Subject-3

GSM42758

Healthy Control Subject-4

GSM42769

Healthy Control Subject-5

GSM42782

Enriched Monocyte Population

Healthy Control Subject-1

GSM42793

Healthy Control Subject-2

GSM42748

Healthy Control Subject-3

GSM42781

Healthy Control Subject-4

GSM42791

Healthy Control Subject-5

GSM42766