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<P>Pirottin <I>et al</I>. 10.1073/pnas.0502426102.</TD>
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<H2>Supporting Information</H2>
<H4>Files in this Data Supplement:</H4>
<A HREF="#F5">Supporting Figure 5</A><BR>
<A HREF="#F6">Supporting Figure 6</A><BR>
<A HREF="#F4">Supporting Figure 7</A><BR>


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<A NAME="F5"><A href="{__picrender__}pnas_102_18_6413__1.pdf">Supporting Figure 5</A></A>
<B><P>Fig. 5.</B> Screening for ES clones having undergone proper gene targeting on the Y chromosome of the pPNTdloxUP (<I>A</I>) and pPNTdloxTSPY (<I>B</I>) constructs. Shown are the position of the primer pairs used for long-template PCR screening (LTPCR) and the position of the restriction sites and probe used for Southern blotting. The result of the PCR assays and Southern blotting are shown for the <I>R1-UP-neotk</I> (<I>A</I>) and <I>R1-TSPY-neotk</I> (<I>B</I>) clones and for clones that were considered to be negative (Neg. Clones). The arrows point to the bands of the expected size. Both for &quot;<I>UP</I>&quot; and &quot;<I>TSPY</I>,&quot; most clones that proved positive by PCR appeared to have probably multiple integrations of the transgene in an autosomal locus, explaining the multiple bands observed for the Neg. clones by Southern blotting.</P>
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<A NAME="F6"><A href="{__picrender__}pnas_102_18_6413__2.pdf">Supporing Figure 6</A></A>
<B><P>Fig. 6.</B> Screening for <I>R1-UP-neotk</I> (<I>A</I>) and <I>R1-TSPY-neotk</I> (<I>B</I>) ES clones having undergone proper recombinase-mediated cassette exchange (RMCE) with the mDAFdloxLAP vector. Shown are the position of the primer pairs used for PCR screening and the position of the restriction sites and probe used for Southern blotting. The result of the PCR assays and Southern blotting are shown for one <I>R1-UP-LAP</I> (<I>A</I>) and <I>R1-TSPY-LAP</I> (<I>B</I>) clone each and for clones that were considered to be negative (Neg. Clones). The arrows point to the bands of the expected size.</P>

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<A NAME="F4"><A href="{__picrender__}pnas_102_18_6413__3.pdf">Supporting Figure 7</A></A>
<B><P>Fig. 7.</B> Growth curves over 7 weeks (W4�W10) of <I>BC-CONT</I>, <I>BC-UP-LAP</I>, and <I>BC-TSPY-LAP</I> animals sorted by sex (M and F). Error bars correspond to standard errors of the means computed separately for each sex�genotype�week combination. More thorough comparisons of the growth curves by using mixed model methodology are described in <I>Materials and Methods</I>.</P>