LOCUS supplementary_fi 5730 bp DNA circular SYN 06-DEC-2022 DEFINITION HsSARM1_561_724 in pET-28a(+). ACCESSION . VERSION . KEYWORDS . SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 5730) AUTHORS . TITLE Direct Submission JOURNAL Exported Aug 9, 2023 from SnapGene 6.2.2 https://www.snapgene.com COMMENT Resistance markers: Kanamycin This file was created using tools provided by Twist Bioscience FEATURES Location/Qualifiers source 1..5730 /mol_type="other DNA" /organism="synthetic DNA construct" source join(478..5730,1..477) /organism="HsSARM1_561_724 in pET-28a(+)" misc_feature 1..78 /label=lacIQ_promoter /label=nonstandard type: misc_feature_ misc_feature 387..405 /label=T7 /label=nonstandard type: misc_feature_ misc_feature 405..432 /label=lacO /label=nonstandard type: misc_feature_ misc_feature 445..467 /label=RBS_3 /label=nonstandard type: misc_feature_ misc_feature 450..466 /label=T7_transl_en_RBS /label=nonstandard type: misc_feature_ CDS 474..974 /codon_start=1 /label=HsSARM1 561-724 /translation="MGTPDVFISYRRNSGSQLASLLKVHLQLHGFSVFIDVEKLEAGKF EDKLIQSVMGARNFVLVLSPGALDKCMQDHDCKDWVHKEIVTALSCGKNIVPIIDGFEW PEPQVLPEDMQAVLTFNGIKWSHEYQEATIEKIIRFLQGRSSRDSSAGSDTSLEGAAPM GPT" misc_feature 478..974 /label=insert /label=nonstandard type: misc_feature_ misc_feature join(975..5730,1..477) /label=vector_backbone-497-5730 /label=nonstandard type: misc_feature_ misc_feature 981..998 /label=6x_His_tag /label=nonstandard type: misc_feature_ misc_feature 1166..1472 /label=f1 /label=nonstandard type: misc_feature_ misc_feature 1696..2511 /label=kan /label=nonstandard type: misc_feature_ misc_feature 2633..3221 /label=colE1_high_copy /label=nonstandard type: misc_feature_ misc_feature 3648..3839 /label=ROP /label=nonstandard type: misc_feature_ misc_feature 4614..4635 /label=CAP_binding /label=nonstandard type: misc_feature_ misc_feature join(4648..5730,1..9) /label=lacI /label=nonstandard type: misc_feature_ ORIGIN 1 attcaccacc ctgaattgac tctcttccgg gcgctatcat gccataccgc gaaaggtttt 61 gcgccattcg atggtgtccg ggatctcgac gctctccctt atgcgactcc tgcattagga 121 agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca 181 aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac 241 aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat 301 aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga 361 ggatcgagat ctcgatcccg cgaaattaat acgactcact ataggggaat tgtgagcgga 421 taacaattcc cctctagaaa taattttgtt taactttaag aaggagatat accatgggca 481 ctccggacgt gtttatctca taccgacgta actcaggttc acagctggcc agtcttctta 541 aagtgcacct gcaattacac ggctttagtg tgtttattga cgttgagaaa ctggaagcgg 601 gcaaatttga agataaactt atccagtcgg ttatgggagc gcgtaatttc gttctggttt 661 tgtcccctgg tgcactggat aagtgcatgc aagaccatga ctgtaaggac tgggtgcata 721 aggaaatagt aaccgcatta tcgtgtggca agaacattgt accgataatc gatggttttg 781 aatggccaga accacaggtg ctgcctgaag atatgcaagc cgttttaacc tttaatggca 841 ttaagtggtc acacgaatac caggaagcaa ccatagaaaa gatcattcga ttcctgcagg 901 gcagatcatc gcgtgacagt tccgctggta gcgacacgtc cctcgagggt gcagcgccaa 961 tgggtcccac ataactcgag caccaccacc accaccactg agatccggct gctaacaaag 1021 cccgaaagga agctgagttg gctgctgcca ccgctgagca ataactagca taaccccttg 1081 gggcctctaa acgggtcttg aggggttttt tgctgaaagg aggaactata tccggattgg 1141 cgaatgggac gcgccctgta gcggcgcatt aagcgcggcg ggtgtggtgg ttacgcgcag 1201 cgtgaccgct acacttgcca gcgccctagc gcccgctcct ttcgctttct tcccttcctt 1261 tctcgccacg ttcgccggct ttccccgtca agctctaaat cgggggctcc ctttagggtt 1321 ccgatttagt gctttacggc acctcgaccc caaaaaactt gattagggtg atggttcacg 1381 tagtgggcca tcgccctgat agacggtttt tcgccctttg acgttggagt ccacgttctt 1441 taatagtgga ctcttgttcc aaactggaac aacactcaac cctatctcgg tctattcttt 1501 tgatttataa gggattttgc cgatttcggc ctattggtta aaaaatgagc tgatttaaca 1561 aaaatttaac gcgaatttta acaaaatatt aacgcttaca atttaggtgg cacttttcgg 1621 ggaaatgtgc gcggaacccc tatttgttta tttttctaaa tacattcaaa tatgtatccg 1681 ctcatgaatt aattcttaga aaaactcatc gagcatcaaa tgaaactgca atttattcat 1741 atcaggatta tcaataccat atttttgaaa aagccgtttc tgtaatgaag gagaaaactc 1801 accgaggcag ttccatagga tggcaagatc ctggtatcgg tctgcgattc cgactcgtcc 1861 aacatcaata caacctatta atttcccctc gtcaaaaata aggttatcaa gtgagaaatc 1921 accatgagtg acgactgaat ccggtgagaa tggcaaaagt ttatgcattt ctttccagac 1981 ttgttcaaca ggccagccat tacgctcgtc atcaaaatca ctcgcatcaa ccaaaccgtt 2041 attcattcgt gattgcgcct gagcgagacg aaatacgcga tcgctgttaa aaggacaatt 2101 acaaacagga atcgaatgca accggcgcag gaacactgcc agcgcatcaa caatattttc 2161 acctgaatca ggatattctt ctaatacctg gaatgctgtt ttcccgggga tcgcagtggt 2221 gagtaaccat gcatcatcag gagtacggat aaaatgcttg atggtcggaa gaggcataaa 2281 ttccgtcagc cagtttagtc tgaccatctc atctgtaaca tcattggcaa cgctaccttt 2341 gccatgtttc agaaacaact ctggcgcatc gggcttccca tacaatcgat agattgtcgc 2401 acctgattgc ccgacattat cgcgagccca tttataccca tataaatcag catccatgtt 2461 ggaatttaat cgcggcctag agcaagacgt ttcccgttga atatggctca taacacccct 2521 tgtattactg tttatgtaag cagacagttt tattgttcat gaccaaaatc ccttaacgtg 2581 agttttcgtt ccactgagcg tcagaccccg tagaaaagat caaaggatct tcttgagatc 2641 ctttttttct gcgcgtaatc tgctgcttgc aaacaaaaaa accaccgcta ccagcggtgg 2701 tttgtttgcc ggatcaagag ctaccaactc tttttccgaa ggtaactggc ttcagcagag 2761 cgcagatacc aaatactgtc cttctagtgt agccgtagtt aggccaccac ttcaagaact 2821 ctgtagcacc gcctacatac ctcgctctgc taatcctgtt accagtggct gctgccagtg 2881 gcgataagtc gtgtcttacc gggttggact caagacgata gttaccggat aaggcgcagc 2941 ggtcgggctg aacggggggt tcgtgcacac agcccagctt ggagcgaacg acctacaccg 3001 aactgagata cctacagcgt gagctatgag aaagcgccac gcttcccgaa gggagaaagg 3061 cggacaggta tccggtaagc ggcagggtcg gaacaggaga gcgcacgagg gagcttccag 3121 ggggaaacgc ctggtatctt tatagtcctg tcgggtttcg ccacctctga cttgagcgtc 3181 gatttttgtg atgctcgtca ggggggcgga gcctatggaa aaacgccagc aacgcggcct 3241 ttttacggtt cctggccttt tgctggcctt ttgctcacat gttctttcct gcgttatccc 3301 ctgattctgt ggataaccgt attaccgcct ttgagtgagc tgataccgct cgccgcagcc 3361 gaacgaccga gcgcagcgag tcagtgagcg aggaagcgga agagcgcctg atgcggtatt 3421 ttctccttac gcatctgtgc ggtatttcac accgcaatgg tgcactctca gtacaatctg 3481 ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg 3541 gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg 3601 gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca 3661 ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc 3721 gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt 3781 aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact 3841 gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag 3901 aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag 3961 ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc 4021 cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg 4081 cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca 4141 cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg 4201 cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag 4261 cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggggc cgccatgccg 4321 gcgataatgg cctgcttctc gccgaaacgt ttggtggcgg gaccagtgac gaaggcttga 4381 gcgagggcgt gcaagattcc gaataccgca agcgacaggc cgatcatcgt cgcgctccag 4441 cgaaagcggt cctcgccgaa aatgacccag agcgctgccg gcacctgtcc tacgagttgc 4501 atgataaaga agacagtcat aagtgcggcg acgatagtca tgccccgcgc ccaccggaag 4561 gagctgactg ggttgaaggc tctcaagggc atcggtcgag atcccggtgc ctaatgagtg 4621 agctaactta cattaattgc gttgcgctca ctgcccgctt tccagtcggg aaacctgtcg 4681 tgccagctgc attaatgaat cggccaacgc gcggggagag gcggtttgcg tattgggcgc 4741 cagggtggtt tttcttttca ccagtgagac gggcaacagc tgattgccct tcaccgcctg 4801 gccctgagag agttgcagca agcggtccac gctggtttgc cccagcaggc gaaaatcctg 4861 tttgatggtg gttaacggcg ggatataaca tgagctgtct tcggtatcgt cgtatcccac 4921 taccgagata tccgcaccaa cgcgcagccc ggactcggta atggcgcgca ttgcgcccag 4981 cgccatctga tcgttggcaa ccagcatcgc agtgggaacg atgccctcat tcagcatttg 5041 catggtttgt tgaaaaccgg acatggcact ccagtcgcct tcccgttccg ctatcggctg 5101 aatttgattg cgagtgagat atttatgcca gccagccaga cgcagacgcg ccgagacaga 5161 acttaatggg cccgctaaca gcgcgatttg ctggtgaccc aatgcgacca gatgctccac 5221 gcccagtcgc gtaccgtctt catgggagaa aataatactg ttgatgggtg tctggtcaga 5281 gacatcaaga aataacgccg gaacattagt gcaggcagct tccacagcaa tggcatcctg 5341 gtcatccagc ggatagttaa tgatcagccc actgacgcgt tgcgcgagaa gattgtgcac 5401 cgccgcttta caggcttcga cgccgcttcg ttctaccatc gacaccacca cgctggcacc 5461 cagttgatcg gcgcgagatt taatcgccgc gacaatttgc gacggcgcgt gcagggccag 5521 actggaggtg gcaacgccaa tcagcaacga ctgtttgccc gccagttgtt gtgccacgcg 5581 gttgggaatg taattcagct ccgccatcgc cgcttccact ttttcccgcg ttttcgcaga 5641 aacgtggctg gcctggttca ccacgcggga aacggtctga taagagacac cggcatactc 5701 tgcgacatcg tataacgtta ctggtttcac //