Trichobotrys was introduced by Penzig and Saccardo (1902) to accommodate the type species T.pannosus [as ‘pannosa’]. The genus is scarcely known and only five species are available in Index Fungorum (http://www.indexfungorum.org; accessed on 25 May 2023), of which only T.effusus [as ‘effusa’] has molecular data available in GenBank. The genus is known as only a hyphomycetous asexual morph and is characterised by dark brown to black, effuse to velvety colonies, partly immersed to superficial mycelium, non-stromatic, macronematous, mononematous, dark brown to reddish-brown, verruculose or echinulate conidiophores, bearing short, smooth, fertile, often unciform lateral branches, with sterile, setiform apex, polyblastic, integrated, terminal or discrete, determinate, ellipsoidal, spherical or subspherical conidiogenous cells and catenated, in branched acropetal chains, spherical, brown, aseptate, verruculose or minutely echinulate conidia (Ellis 1971; D’Souza and Bhat 2001). The taxonomic classification of the genus is doubtful due to the lack of molecular phylogeny. Recently, Wijayawardene et al. (2022b) treated Trichobotrys as Ascomycota genus incertae sedis, pending future study. In the present study, the novel species, T.sinensis is introduced and the phylogenetic analyses demonstrated the genus affinity in Dictyosporiaceae.