{"text_format":"3","workbook":"xlsx","n_sheets":1,"sheets":["Analysis II"],"not_rendered":{"macros":1,"defined_names":5},"read":"whole","date_system":"1900"}

{"sheet":"Analysis II","index":0,"n_rows":223,"n_cols":11,"rows_kept":223,"merged":[[0,1,0,10],[1,1,2,1],[1,2,2,2],[1,3,1,9],[1,10,2,10]]}
["{remove this column]","Table S1. ELISA screening of B cell supernatants"]
[null,"Ranking","B Cell ID","ELISA detection for proteins or peptides",{"$nulls":6},"Generated recombinant antibody clones"]
["suggested headers",null,null,"p-S65-Ub protein","Ub protein                     (non-phospho)","p-S65-Ub peptide","p-S65-Ub BSA-conj peptide","Ub peptide                    (non-phospho)","p-S65-PRKN peptide","PRKN peptide               (non-phospho)"]
[5,1,"19G71",2.269,0.054,1.846,1.789,0.05,0.05,0.049,"-"]
[6,2,"10F41",2.262,0.051,1.976,2.157,0.046,0.047,0.048,"2H1K2, 2H2K2"]
[7,3,"22A91",2.214,0.051,1.979,1.888,0.048,0.047,0.049,"3H1K2"]
[11,4,"22C11",2.192,0.056,1.821,1.834,0.048,0.048,0.048,"4H1K1, 4H1K3"]
[14,5,"40C71",2.114,0.047,1.794,1.495,0.05,0.05,0.051,"5H2K3"]
[15,6,"4B31",2.107,0.046,1.472,1.425,0.048,0.048,0.053,"6H3K2"]
[16,7,"44B51",2.104,0.053,1.909,1.99,0.161,0.049,0.056,"7H1K2"]
[19,8,"11G61",2.085,0.051,1.931,1.773,0.05,0.052,0.049,"8H1K3"]
[23,9,"14D101",2.049,0.053,1.62,1.719,0.049,0.049,0.049,"9H3K1"]
[25,10,"2E11",2.045,0.049,1.577,1.551,0.052,0.047,0.06,"10H3K1"]
[28,11,"22H51",2.016,0.05,1.92,2.134,0.051,0.047,0.055,"11H2K3"]
[29,12,"44D81",2.003,0.048,2.005,2.108,0.132,0.051,0.052,"-"]
[30,13,"5A61",1.988,0.049,1.591,1.621,0.047,0.048,0.05,"13H3K1"]
[31,14,"24H101",1.986,0.051,1.51,1.357,0.052,0.05,0.051,"14H2K3, 14H3K2"]
[32,15,"44D111",1.975,0.05,1.866,2.096,0.165,0.05,0.052,"15H1K2"]
[33,16,"15F81",1.974,0.05,1.594,1.236,0.049,0.048,0.049,"16H1K2, 16H3K1"]
[34,17,"5D61",1.963,0.049,1.661,1.522,0.051,0.048,0.055,"17H1K3"]
[35,18,"41E101",1.948,0.047,1.811,1.516,0.048,0.048,0.049,"18H2K3, 18H3K2"]
[37,19,"1F41",1.933,0.049,1.451,1.509,0.049,0.049,0.051,"19H1K1, 19H3K2"]
[40,20,"4E101",1.92,0.049,1.507,1.502,0.049,0.047,0.054,"20H1K2, 20H2K3"]
[42,21,"14C21",1.9,0.049,1.781,1.672,0.05,0.048,0.056,"21H3K1"]
[43,22,"23C111",1.875,0.05,1.539,1.493,0.049,0.049,0.049,"-"]
[44,23,"44E11",1.87,0.052,1.689,1.843,0.077,0.071,0.05,"23H2K1"]
[45,24,"8B31",1.865,0.047,1.635,1.564,0.049,0.052,0.05,"24H1K1"]
[46,25,"9F81",1.854,0.048,1.4,1.35,0.046,0.048,0.049,"-"]
[47,26,"24A71",1.823,0.052,1.608,1.523,0.048,0.052,0.052,"26H3K3, 26H3K3"]
[48,27,"1D11",1.818,0.048,1.556,1.553,0.047,0.048,0.05,"27H2K1"]
[49,28,"7D61",1.817,0.051,1.311,1.427,0.052,0.049,0.058,"28H2K2"]
[50,29,"3C111",1.803,0.051,1.584,1.676,0.048,0.049,0.05,"29H1K3, 29H2K2"]
[52,30,"34E21",1.788,0.049,1.819,1.567,0.054,0.053,0.051,"30H3K1"]
[53,31,"15E31",1.772,0.055,1.296,1.166,0.05,0.049,0.053,"31H2K2"]
[54,32,"28D51",1.771,0.052,1.009,1.543,0.048,0.049,0.049,"32H1K3"]
[55,33,"15E91",1.771,0.049,0.728,0.807,0.053,0.048,0.056,"33H1K3"]
[56,34,"27E31",1.762,0.05,1.486,1.493,0.048,0.05,0.049,"34H2K2"]
[57,35,"6H71",1.758,0.069,1.229,1.106,0.182,0.049,0.053,"-"]
[60,36,"28H1",1.73,0.047,1.651,1.391,0.054,0.049,0.07,"36H1K1"]
[145,37,"2G52",0.635,0.105,0.084,0.119,0.048,0.047,0.049,"-"]
[155,38,"5C102",0.443,0.046,0.149,0.148,0.049,0.048,0.05,"-"]
[157,39,"37G12",0.433,0.049,0.157,0.135,0.048,0.05,0.053,"-"]
[158,40,"2B12",0.423,0.048,0.052,0.075,0.048,0.048,0.047,"40H3K2"]
[58,41,"3F83",1.754,0.047,1.367,1.278,0.067,1.831,0.062,"41H1K3"]
[81,42,"17G43",1.449,0.05,0.602,0.408,0.072,1.25,0.071,"-"]
[118,43,"6B83",1.072,0.065,0.45,0.348,0.053,0.711,0.053,"-"]
[141,44,"21E113",0.715,0.052,0.221,0.144,0.05,0.335,0.053,"-"]
[140,45,"30G34",0.727,0.047,0.497,0.415,0.047,0.046,0.048,"45H1K1"]
[123,46,"25B94",1.023,0.049,0.68,1.636,0.046,0.047,0.047,"46H2K2"]
[124,47,"31A74",1.019,0.049,0.856,0.797,0.048,0.049,0.051,"47H1K1, 47H2K1"]
[126,48,"37F14",0.95,0.048,0.903,0.686,0.048,0.051,0.051,"48H2K1"]
[1,null,"44A3",2.387,0.062,2.243,2.363,0.453,0.048,0.053]
[2,null,"44B6",2.322,0.055,2.091,2.028,0.476,0.058,0.06]
[3,null,"44B3",2.292,0.057,2.217,2.332,1.157,0.059,0.077]
[4,null,"44B7",2.288,0.051,2.031,2.144,0.896,0.051,0.058]
[8,null,"5D2",2.207,0.051,1.955,1.803,0.49,0.049,0.052]
[9,null,"44C6",2.202,0.051,1.956,1.961,0.35,0.05,0.055]
[10,null,"44C4",2.199,0.052,1.881,1.875,0.253,0.05,0.056]
[12,null,"5C6",2.169,0.568,1.912,1.768,0.566,0.049,0.049]
[13,null,"44D2",2.117,0.065,1.929,1.966,1.472,0.061,0.07]
[17,null,"44D5",2.093,0.25,2.09,2.432,1.409,0.348,0.283]
[18,null,"44C5",2.09,0.055,1.637,1.46,0.215,0.048,0.058]
[20,null,"44B4",2.083,0.05,1.952,1.913,1.48,0.048,0.053]
[21,null,"10G11",2.073,0.276,1.816,1.779,0.691,0.22,0.415]
[22,null,"44G7",2.06,0.063,2.079,2.067,0.843,0.051,0.055]
[24,null,"44F9",2.048,0.052,1.765,1.844,1.547,0.049,0.051]
[26,null,"44A4",2.039,0.057,2.203,2.18,2.06,0.053,0.052]
[27,null,"44F6",2.03,0.052,1.849,1.772,1.551,0.049,0.055]
[36,null,"44A1",1.942,0.081,1.97,2.306,0.459,0.051,0.054]
[38,null,"31B7",1.926,0.051,1.872,1.588,0.387,0.059,0.072]
[39,null,"44C8",1.925,0.049,2.083,2.207,1.998,0.05,0.055]
[41,null,"44F8",1.906,0.05,1.809,1.85,1.433,0.05,0.052]
[51,null,"44F10",1.798,0.05,1.593,1.304,0.372,0.05,0.051]
[59,null,"31D10",1.739,0.048,1.146,0.911,0.048,0.048,0.048]
[61,null,"12B6",1.728,0.048,1.253,1.184,0.048,0.049,0.049]
[62,null,"34H4",1.719,0.048,1.445,1.374,0.048,0.054,0.049]
[63,null,"18D5",1.664,0.049,1.387,1.313,0.051,0.048,0.053]
[64,null,"44E9",1.661,0.05,1.945,1.986,0.197,0.055,0.063]
[65,null,"3H8",1.658,0.046,1.25,1.074,0.047,0.047,0.049]
[66,null,"44F4",1.645,0.053,1.854,1.727,1.031,1.401,1.023]
[67,null,"27A6",1.621,0.064,1.413,1.204,0.05,0.049,0.048]
[68,null,"22A8",1.602,0.057,0.922,0.652,0.064,0.051,0.06]
[69,null,"39D5",1.571,0.048,1.135,1.028,0.048,0.049,0.049]
[70,null,"3D1",1.55,0.047,1.103,1.03,0.048,0.05,0.049]
[71,null,"11H6",1.535,0.048,0.94,0.841,0.047,0.047,0.048]
[72,null,"6B6",1.529,0.048,1.676,1.647,0.102,0.049,0.052]
[73,null,"31C2",1.528,0.047,0.961,0.645,0.049,0.049,0.051]
[74,null,"44H8",1.518,0.051,0.833,0.746,0.094,0.05,0.053]
[75,null,"44C7",1.508,0.056,1.922,1.98,2.093,0.059,0.057]
[76,null,"28C4",1.507,0.051,1.023,0.841,0.053,0.048,0.052]
[77,null,"44F3",1.481,0.085,1.664,1.423,1.298,0.361,0.055]
[78,null,"44B9",1.469,0.053,1.445,1.706,0.97,0.051,0.056]
[79,null,"44C11",1.466,0.052,1.585,1.476,0.775,0.054,0.055]
[80,null,"1B11",1.458,0.047,0.837,0.66,0.05,0.048,0.054]
[82,null,"25B11",1.448,0.05,0.912,0.622,0.048,0.049,0.049]
[83,null,"15G10",1.438,0.049,0.981,0.835,0.049,0.05,0.05]
[84,null,"21B9",1.434,0.048,1.429,1.253,1.254,0.049,0.06]
[85,null,"13D4",1.428,0.051,0.815,0.672,0.061,0.051,0.057]
[86,null,"39E5",1.415,0.047,0.549,1.29,0.048,0.048,0.05]
[87,null,"44A11",1.41,0.059,1.768,1.379,1.005,0.051,0.054]
[88,null,"44G10",1.407,0.05,1.175,1.175,0.056,0.048,0.049]
[89,null,"35D10",1.395,0.048,1.419,0.996,0.05,0.05,0.047]
[90,null,"37G3",1.395,0.048,0.715,0.489,0.056,0.048,0.05]
[91,null,"32B2",1.393,0.046,1.448,1.389,0.046,0.049,0.047]
[92,null,"19G9",1.373,0.05,1.046,0.91,0.049,0.049,0.049]
[93,null,"30H4",1.356,0.047,1.14,0.883,0.048,0.048,0.05]
[94,null,"1H3",1.347,0.048,0.763,0.67,0.05,0.049,0.048]
[95,null,"25D10",1.342,0.051,0.904,0.705,0.049,0.052,0.051]
[96,null,"29C11",1.331,0.049,1,1.04,0.049,0.048,0.049]
[97,null,"37D10",1.326,0.047,1.389,0.986,0.05,0.054,0.049]
[98,null,"28D7",1.322,0.058,0.937,0.874,0.048,0.048,0.05]
[99,null,"2B11",1.322,0.048,0.675,0.587,0.047,0.047,0.054]
[100,null,"44C1",1.31,0.052,2.059,2.01,0.053,0.05,0.049]
[101,null,"24A10",1.304,0.05,0.654,0.544,0.048,0.048,0.048]
[102,null,"13A8",1.302,0.049,0.652,0.493,0.05,0.051,0.055]
[103,null,"28F11",1.3,0.047,1.21,0.989,0.05,0.047,0.055]
[104,null,"11B3",1.292,0.048,0.782,0.778,0.05,0.047,0.051]
[105,null,"33A5",1.268,0.049,0.764,0.419,0.05,0.051,0.05]
[106,null,"20G5",1.245,0.049,0.631,0.59,0.047,0.049,0.053]
[107,null,"35D5",1.241,0.047,1.073,0.873,0.07,0.061,0.05]
[108,null,"22E9",1.233,0.052,0.758,0.917,0.048,0.047,0.047]
[109,null,"44A2",1.163,0.055,1.709,1.642,1.69,0.054,0.133]
[110,null,"44E4",1.153,0.078,2.08,2.087,1.758,0.101,0.134]
[111,null,"3G1",1.146,0.047,0.681,0.484,0.134,0.048,0.053]
[112,null,"10F10",1.141,0.049,0.771,0.646,0.049,0.077,0.05]
[113,null,"11C1",1.141,0.047,0.512,0.48,0.049,0.048,0.048]
[114,null,"32D1",1.109,0.047,0.954,0.675,0.051,0.047,0.05]
[115,null,"24F4",1.106,0.047,0.687,0.522,0.048,0.049,0.048]
[116,null,"2H6",1.097,0.048,0.671,0.547,0.048,0.05,0.049]
[117,null,"22B6",1.096,0.051,0.433,0.427,0.048,0.05,0.057]
[119,null,"27C9",1.069,0.048,0.88,0.861,0.047,0.048,0.049]
[120,null,"25H5",1.067,0.051,0.65,0.528,0.051,0.049,0.049]
[121,null,"40D5",1.054,0.062,0.352,0.315,0.049,0.049,0.075]
[122,null,"42H10",1.041,0.046,0.516,0.358,0.052,0.051,0.05]
[125,null,"8H4",1.012,0.047,0.313,1.106,0.048,0.048,0.051]
[127,null,"44E2",0.929,0.061,1.819,1.79,1.53,0.05,0.066]
[128,null,"29G9",0.91,0.047,0.347,0.238,0.05,0.047,0.048]
[129,null,"44H1",0.899,0.049,1.518,1.438,1.054,0.047,0.051]
[130,null,"12F1",0.877,0.049,0.372,0.339,0.046,0.049,0.052]
[131,null,"44B10",0.861,0.054,0.571,0.338,0.056,0.05,0.049]
[132,null,"38A11",0.861,0.048,0.479,0.313,0.047,0.051,0.047]
[133,null,"9E6",0.858,0.771,1.027,0.683,1.033,0.663,1.155]
[134,null,"18D8",0.854,0.05,0.215,0.238,0.048,0.049,0.048]
[135,null,"44F7",0.846,0.051,0.597,0.418,0.272,0.05,0.06]
[136,null,"21G2",0.837,0.054,0.259,0.158,0.047,0.051,0.053]
[137,null,"4B4",0.823,0.05,0.334,0.288,0.047,0.048,0.048]
[138,null,"44H6",0.759,0.054,1.533,1.493,0.398,0.058,0.061]
[139,null,"44D6",0.751,0.053,1.351,1.407,0.784,1.1,0.432]
[142,null,"30A10",0.712,0.047,0.37,0.303,0.048,0.048,0.048]
[143,null,"28H8",0.666,0.048,0.34,0.212,0.048,0.048,0.055]
[144,null,"44B8",0.655,0.052,1.553,1.258,0.376,0.048,0.058]
[146,null,"25H10",0.629,0.048,0.244,0.197,0.046,0.049,0.047]
[147,null,"34G11",0.607,0.047,0.223,0.146,0.051,0.049,0.069]
[148,null,"35A7",0.595,0.046,0.202,0.168,0.049,0.047,0.047]
[149,null,"44G8",0.576,0.05,1.236,0.836,0.89,0.54,0.068]
[150,null,"44E6",0.568,0.05,0.392,0.271,0.061,0.048,0.054]
[151,null,"44G9",0.523,0.049,1.757,2.075,1.494,0.049,0.051]
[152,null,"44E5",0.507,0.053,2.122,2.101,0.446,0.05,0.052]
[153,null,"28C5",0.464,0.049,0.276,0.233,0.048,0.05,0.057]
[154,null,"30E6",0.444,0.046,0.628,1.296,0.401,0.05,0.05]
[156,null,"44G3",0.437,0.061,0.177,0.968,0.077,0.055,0.054]
[159,null,"44H7",0.415,0.054,1.48,1.608,0.713,0.051,0.053]
[160,null,"25A6",0.414,0.051,0.168,0.166,0.05,0.048,0.05]
[161,null,"10G5",0.391,0.051,0.17,0.143,0.049,0.13,0.053]
[162,null,"44G6",0.381,0.055,1.474,1.161,0.753,0.05,0.053]
[163,null,"44H9",0.37,0.054,0.187,0.134,0.051,0.214,0.05]
[164,null,"4A11",0.355,0.047,0.101,0.093,0.046,0.049,0.048]
[165,null,"39B1",0.335,0.047,0.116,0.113,0.056,0.051,0.049]
[166,null,"31E4",0.325,0.047,0.098,0.079,0.048,0.048,0.047]
[167,null,"29D6",0.296,0.047,0.15,0.11,0.049,0.047,0.047]
[168,null,"44C3",0.25,0.054,1.48,1.107,0.973,0.067,0.059]
[169,null,"5C8",0.242,0.046,0.096,0.094,0.048,0.049,0.048]
[170,null,"27E4",0.233,0.051,0.133,0.129,0.051,0.048,0.049]
[171,null,"44E7",0.173,0.063,1.279,1.295,1.279,0.049,0.064]
[172,null,"25F2",0.165,0.051,0.092,0.067,0.051,0.049,0.048]
[173,null,"27A8",0.153,0.051,0.083,0.069,0.046,0.047,0.05]
[174,null,"44G1",0.149,0.215,1.189,1.03,0.352,0.09,0.145]
[175,null,"44H5",0.147,0.056,1.647,1.599,0.915,0.049,0.058]
[176,null,"2F6",0.143,0.047,0.696,0.52,0.812,0.065,0.194]
[177,null,"32F5",0.135,0.048,0.06,0.061,0.049,0.048,0.049]
[178,null,"44C2",0.124,0.05,0.787,1.261,0.162,0.05,0.053]
[179,null,"44H10",0.12,0.05,0.083,0.067,0.049,0.048,0.048]
[180,null,"44F5",0.104,0.064,1.678,1.331,1.269,0.076,0.136]
[181,null,"44B2",0.097,0.062,0.749,1.304,0.491,0.049,0.055]
[182,null,"44D7",0.081,0.056,0.766,0.361,0.334,0.051,0.052]
[183,null,"44G11",0.069,0.083,0.823,0.407,0.277,0.104,0.122]
[184,null,"44H2",0.061,0.048,1.194,1.427,0.42,0.047,0.05]
[185,null,"44A8",0.06,0.055,1.718,1.507,1.216,0.077,0.079]
[186,null,"44A7",0.06,0.095,0.494,0.232,0.164,0.053,0.053]
[187,null,"44E3",0.059,0.05,0.425,0.772,0.05,0.049,0.05]
[188,null,"44E10",0.059,0.059,0.898,0.479,0.237,0.053,0.055]
[189,null,"44B11",0.056,0.054,1.695,1.373,1.533,0.057,0.073]
[190,null,"44D1",0.054,0.05,1.109,0.918,1.09,0.05,0.051]
[191,null,"44H4",0.054,0.052,0.237,0.157,0.113,0.048,0.051]
[192,null,"44D3",0.053,0.05,1.757,1.654,1.686,0.051,0.056]
[193,null,"44F11",0.052,0.051,2.096,2.075,0.544,0.052,0.058]
[194,null,"44D10",0.052,0.051,1.615,1.533,0.258,0.05,0.052]
[195,null,"44B1",0.052,0.049,1.422,1.307,1.055,0.073,0.115]
[196,null,"44D4",0.052,0.052,1.09,0.799,0.967,0.05,0.056]
[197,null,"44F1",0.052,0.051,0.874,0.773,0.545,0.051,0.063]
[198,null,"44F2",0.052,0.052,0.941,0.615,0.398,0.047,0.055]
[199,null,"44H3",0.052,0.049,0.196,0.121,0.092,0.048,0.113]
[200,null,"14E7",0.052,0.052,0.047,0.048,0.047,0.052,0.048]
[201,null,"44G4",0.051,0.052,1.726,1.754,0.976,0.05,0.052]
[202,null,"44A6",0.051,0.053,1.227,1.012,0.373,0.056,0.065]
[203,null,"44H11",0.051,0.05,1.136,0.735,0.489,0.049,0.051]
[204,null,"44G5",0.05,0.053,1.877,1.748,0.775,0.049,0.056]
[205,null,"39G9",0.05,0.051,0.048,0.047,0.046,0.048,0.048]
[206,null,"44E8",0.049,0.057,1.777,1.731,0.979,0.069,0.085]
[207,null,"44E11",0.049,0.051,1.683,1.397,1.547,0.049,0.056]
[208,null,"44G2",0.049,0.072,1.195,0.87,0.121,0.049,0.05]
[209,null,"44D9",0.049,0.049,0.116,0.081,0.059,0.05,0.052]
[210,null,"44C9",0.049,0.05,0.091,0.064,0.065,0.049,0.082]
[211,null,"44C10",0.048,0.05,0.272,0.813,0.049,0.048,0.049]
[212,null,"44A10",0.048,0.049,0.13,0.093,0.047,0.05,0.049]
[213,null,"44A5",0.048,0.049,0.158,0.084,0.072,0.049,0.051]
[214,null,"39G11",0.048,0.063,0.047,0.052,0.048,0.049,0.058]
[215,null,"44A9",0.047,0.05,1.48,1.115,0.269,0.053,0.051]
[null,"Ub - ubiquitin, \"-\" - clone not available."]
["Legend: ","1 B cells that detect both p-S65-Ub protein and peptide. "]
[null,"2 B cells that only detect p-S65-Ub protein but not peptide. "]
[null,"3 B cells that detect both p-S65-Ub protein and peptide but additionally also recognize p-S65-PRKN peptide. "]
[null,"4 B cells that weakly detect p-S65-Ub protein and peptide. "]