Synthetic DNA oligonucleotides

Oligo-directed RNase H digestion

NameSequenceTarget gene
16S5’ATCAGGACATCCCGATGGTGCAG3’ribosomal RNA, large subunit
ACTA15’AACTTCCGTTGCTGCCATCGTAAA3’actin, alpha 1, skeletal muscle
ATP65’CGCTGTCGCCTTAATCCAAGCCTA3’ATPase, subunits 6-8
CKM5’AATGGAGAGCTCCCAGCACACTCG3’creatine kinase, muscle
COX25’GGGGTATACTACGGTCAATGCTCT3’cytochrome c oxidase, subunit 2
COX35’ACTATCTGCTTCATCCGCCAACTA3’cytochrome c oxidase, subunit 3
GAPD5’AGCCGCACCTTGTCATGTACCAT3’glyceraldehyde-3-phosphate dehydrogenase
HBA15’TGAGCACCGTGCTGACCTCCAAAT3’globin, alpha 1
MYH75’TTGCCACATCTTGATCTGCTCAGC3’myosin, heavy polypeptide 7, cardiac muscle, beta
MYLC25’CCGCACATCCTACAGTTGGAAATC3’myosin, light polypeptide 2, regulatory, cardiac, slow
MYL25’CTCGTCTTTGCAGAGTGGTCCCTG3’myosin, light polypeptide 2, regulatory, cardiac, slow
NADH25’CCCTTACCACGCTACTCCTACCTA3’NADH dehydrogenase, subunit 2
NADH35’GTCTGGCCTATGAGTGACTACAAA3’NADH dehydrogenase, subunit 3
TNNI15’GCTCTGAGCTCCTGCTTCATGCTTC3’troponin I, skeletal, slow
TNNT15’GTGTTTGTCCCATCGGTAGCTTGA3’troponin T1, skeletal, slow
TPM25’AGGAGCAGAAATTGCCAACATTGC3’tropomyosin 2 (beta)

Oligonucleodides directed against mitochondrial genes are coloured.

Cloning the human mitochondrial genomic clones

ClonePrimer forward (H-strand sequence)Primer reverse (L-strand sequence)
Mit 15'AAACGCTTAGCCTAGCCACA3'5'TTGGCTCTCCTTGCAAAGTT3'
Mit 25'GCTAAACCTAGCCCCAAACC3'5'GGCAGGTCAATTTCACTGGT3'
Mit 35'CTGTATGAATGGCTCCACGA3'5'GCCTAGGTTGAGGTTGACCA3'
Mit 45'ATCACCCTCTACATCACCGC3'5'GATGAGTGTGCCTGCAAAGA3'
Mit 55'CCATCATAGCAGGCAGTTGAG3'5'GTTGATGCAGAGTGGGGTTT3'
Mit 65'TCAACTGAACGCAAATCAGC3'5'ATTCCGAAGCCTGGTAGGAT3'
Mit 75'GATTTTTCGGTCACCCTGAA3'5'CGGTTTCTATTTCCTGAGCG3'
Mit 85'TTCATGATCACGCCCTCATA3'5'TTTGGTGAGGGAGGTAGGTG3'
Mit 95'CACCTACCTCCCTCACCAAA3'5'GGTCATTATGTGTTGTCGTGC3'
Mit 105'CCACCAATCACATGCCTATC3'5'CTATGTAGCCGTTGAGTTGTGG3'
Mit 115'CTCCATCTATTGATGAGGGTC3'5'TGGAAAGTCATGTCAGTGGTAG3'
Mit 125'GCTCCCTTCCCCTACTCATC3'5'AGGGGTCGTAAGCCTCTGTT3'
Mit 135'ACAGAGGCTTACGACCCCTT3'5'GCTTGAGTGGAGTAGGGCTG3'
Mit 145'CAGCCCTACTCCACTCAAGC3'5'GTAGGATTGGTGCTGTGGGT3'
Mit 155'GTTTACCACAACCACCACCC3'5'AAGAGAAGTAAGCCGAGGGC3'
Mit 165'GCCCTCGGCTTACTTCTCTT3'5'ATCTCCGGCTTACAAGACTG3'

Semi-quantitative PCR amplification

ESTIdentified geneLEFT PRIMERRIGHT PRIMER
HSPD49915 Breast cancer metastasis-suppressor 1 (BRMS1) mRNA5'CCCTCCTCGAGGCCTGCACA3'5'GGCCTGGCATCTTGCCTCCA3'
HSPD50029Rho GDP dissociation inhibitor (GDI) beta 5'CCGGGCTTTCACCCAACCTG3'5'CCCTCTGGCAGGGGATGTGAC3'
HSPD50353Homo sapiens mRNA; cDNA DKFZp434A109 5'CCTGCTCAGCTCAGGCCCCTTT3'5'CCAGACCTCTCGATGGCCACTT3'
HSPD50480Hypothetical protein FLJ12612 similar to COP95'GGGCTGGGATTAGGGTGGTTCA3'5'GGGCTGCATGAAACATACTTTGGAA3'
HSPD50555Hypothetical protein MGC4368 5'CCATCCTGACCCGCCAGTGC3'5'CCCTGTCCTGCAACGAGGGACT3'
HSPD50628KIAA0806 gene product5'CGGGGCAAATGTGCTTCCTGA3'5'TGGTCAAGCCTGGGATTCTTGC3'
HSPD507285'TCCCAAAACCCCTGGCCTGT3'5'CAAGGGAAAGCTGGCTGCCACA3'
HSPD50730HIRA-interacting protein 35'AGGTGGCCTCCTTGGATGTTGC3'5'CAGACACAGGGCAAGGGGTGCT3'
HSPD50808KIAA0303 protein5'CCAGCCTCCCCCAGCTAGGAA3'5'CGAGCTCTGCCTGGCGGACA3'
HSPD50909Kreisler (mouse) maf-related leucine zipper homolog5'GACGCTCGGCGCCCAAATCT3'5'TGGCGGGGCGGGTATTTACA3'
HSPD51065COPZ2 for nonclathrin coat protein zeta-COP5'GGGCAGATGATGGCGGCTTG3'5'AAGGCCAGGGCCGTGAGAAA3'
CONTROLS
SymbolGeneLEFT PRIMERRIGHT PRIMER
GAPDglyceraldehyde-3-phosphate dehydrogenase5'GAGCACCAGGTGGTCTCCTCTGAC3'5'TGGTACATGACAAGGTGCGGCTC3'
TELEtelethonin, 19 kDa sarcomeric protein/titin-cap5'TGCTGGCCATGGCTGCTTTG3'5'CCCACCATCCTTGCCTCTGC3'
ANKRD2ankyrin repeat domain 2 (stretch responsive muscle)5'TGCCCTGCATGACGCTGTGA3'5'AGGACCCTCCGGCTGCACAC3'

Laveder et al. Supplementary Material