DNA probes spotted on nylon filters

 
1
2
3
4
5
6
7
8
9
10
11
12
A
Mit 1
Mit 2
ACTA1
HBA1
MYL2
CKM
TNNT1
RPL37A
MB
GAPD
MYH7
TPM2
B
Mit 3
Mit 4
TNNI1
RPL41
MYL3
TELE
COX6A2
MYL2
DES
TNNC1
HBB
RPS25
C
Mit 5
Mit 6
TNNC2
TNNI2
RPL37
ALDOA
RPLP1
COX7A1
MYH2A
MYBPC1
TPM1
HSPB1
D
Mit 7
Mit 8
ENO3
CRYAB
RPL31
FHL1
RPL10
MYOZ
TNNT3
COX7C
RPLP2
(MYL3)
E
Mit 9
Mit 10
LOC5454
RPS11
NTRK1
(ESTs)
UBA52
RPL32
TPT1
PYGM
RPL35A
RPS17
F
Mit 11
Mit 12
ATP6E
GENX-3414
SNRPD3
HSF1
SIVA
NME4
STK16
NPY6R
TOB2
PPP4C
G
Mit 13
Mit 14
H2AFO
EIF3S10
BRD4
MOV34
SLC25A1
IL3RA
CD2BP2
HSPD14174
NFKBIA
MGMT
H
Mit 15
Mit 16
Vector
Vector
Cot1 DNA
Cot1 DNA
Arabidopsis
Arabidopsis
Arabidopsis
water
water
TE

      Legend:

Columns 3-12
Rows A-E
  • MOST ABUNDANT MUSCLE TRANSCRIPTS (nuclear encoded)
Columns 3-12
Rows F-G
  • Low abundant muscle transcripts (nuclear encoded)
Columns 1-2
Rows A-H
  • Human mitochondrial genomic clones
Columns 3-12
Row H
  • Controls

Show hybridization results
Laveder et al. Supplementary Material

Detailed lists of DNA probes spotted on nylon filters:

MOST ABUNDANT MUSCLE TRANSCRIPTS (nuclear encoded)
Filter
Gene descriptionGenBankEST
A03
actin, alpha 1, skeletal muscleJ00068F37473
A04
globin, alpha 1V00491F15521
A05
myosin, light polypeptide 2, regulatory, cardiac, slowX66141F20538
A06
creatine kinase, muscleM14780F26514
A07
troponin T1, skeletal, slowS69208F19116
A08
ribosomal protein L37aL06499F31115
A09
myoglobinX00371F28340
A10
glyceraldehyde-3-phosphate dehydrogenaseM33197F18940
A11
myosin, heavy polypeptide 7, cardiac muscle, betaM57965F22655
A12
tropomyosin 2 (beta)X06825F24090
B03
troponin I, skeletal, slowJ04760F25084
B04
ribosomal protein L41Z12962F21077
B05
myosin, light polypeptide 3, alkali; ventricular, skeletal, slowM24122F29280
B06
telethonin, 19 kDa sarcomeric protein/titin-capAJ000491F25768
B07
cytochrome c oxidase subunit VIa polypeptide 2U66875F26881
B08
myosin light chain 2 (clone PWHLC2-24)M21812F24767
B09
desminM63391F25371
B10
troponin C, slowM37984F16174
B11
globin, betaL48932F33863
B12
ribosomal protein S25M64716F20907
C03
troponin C2, fastX07898F26813
C04
troponin I, skeletal, fastL21715F27052
C05
ribosomal protein L37D23661F26277
C06
aldolase A, fructose-bisphosphateM11560F25054
C07
ribosomal protein, large, P1M17886F25783
C08
cytochrome c oxidase subunit VIIa polypeptide 1 (muscle)AF03737F26304
C09
myosin, heavy polypeptide 2, skeletal muscle, adultS73840F25904
C10
myosin-binding protein C, slow-typeX73114F26981
C11
tropomyosin 1 (alpha)M19713F19096
C12
heat shock 27kD protein 1U90906F21894
D03
beta enolase, muscleX56832F25949
D04
crystallin, alpha BS45630F25910
D05
ribosomal protein L31X15940F24394
D06
four and a half LIM domains 1U60115F22733
D07
ribosomal protein L10M64241F26557
D08
FATZ/myozeninAJ27812F21894
D09
troponin T3, skeletal, fastM21984F25928
D10
cytochrome c oxidase subunit VIIcX16560F29987
D11
ribosomal protein, large P2M17887F34186
D12
myosin light chain 3 (MLC-3f)X05451F22511
E03
6.2 kd proteinAJ01100F22523
E04
ribosomal protein S11X06617F25907
E05
tropomyosin (1.3 kb mRNA)X04201F27428
E06
PROVISIONAL: cDNA FLJ22805 fis, clone KAIA2821AK02645F37939
E07
ubiquitin A-52 residue ribosomal protein fusion product 1X56999F27349
E08
ribosomal protein L32X03342F25000
E09
tumor protein, translationally-controlled 1X16064F19628
E10
glycogen phosphorylase, muscle (McArdle syndrome)U94777F25171
E11
ribosomal protein L35aX52966F37831
E12
ribosomal protein S17M13932F24448
LOW ABUNDANT MUSCLE TRANSCRIPTS (nuclear encoded)
Filter
Gene descriptionGenBankEST
F03
ATPase, H+ transporting, lysosomal (vacuolar proton pump) 31kDX76228F26032
F04
genethonin 1AF062534F26188
F05
small nuclear ribonucleoprotein D3 polypeptide (18kD)U15009F25647
F06
heat shock transcription factor 1M64673F25569
F07
CD27-binding (Siva) proteinU82938F26136
F08
non-metastatic cells 4, protein expressed in (NME4), mRNAY07604F24264
F09
serine/threonine kinase 16AJ010872F24431
F10
neuropeptide Y receptor Y6D86519F24590
F11
transducer of ERBB2, 2D64109F24838
F12
protein phosphatase 4 (formerly X), catalytic subunitX70218F26776
G03
H2A histone family, member OL19779F24112
G04
eukaryotic translation initiation factor 3, subunit 10 (theta, 150/170kD)U58046F24895
G05
Bromodomain-containing 4Y12059F24985
G06
COP9 subunit 6 (MOV34 homolog, 34 kD) U70735F25208
G07
solute carrier family 25 (mitochondrial carrier; citrate transporter), member 1U25147F25455
G08
interleukin 3 receptor, alpha (low affinity)M74782F25664
G09
CD2 antigen (cytoplasmic tail)-binding protein 2AF104222F26257
G10
Human mRNA for SB classII histocompatibility antigen alpha-chainX00457F26624
G11
nuclear factor of kappa light polypeptide gene enhancer in B-cells inhibitor, alphaM69043F27600
G12
O-6-methylguanine-DNA methyltransferaseM60761F27739

HUMAN MITOCHONDRIAL GENOMIC CLONES

RefSeq  NC_001807
CloneFilterFromToSpans following gene(s)
Mit 1
A01
7821892ribosomal RNA, small subunit (648-1601)
Mit 2
A02
16712688ribosomal RNA, large subunit (1671-3229), 5' fragment
Mit 3
B01
26253609ribosomal RNA, large subunit (1671-3229), 3' fragment
Mit 4
B02
35084526NADH dehydrogenase, subunit 1 (3305-4262)
Mit 5
C01
49515617NADH dehydrogenase, subunit 2 (4470-5511)
Mit 6
C02
56136661cytochrome c oxidase, subunit 1 (5901-7517), 5' fragment
Mit 7
D01
66107775cytochrome c oxidase, subunit 1 (5901-7517), 3' fragment
Mit 8
D02
76538484cytochrome c oxidase, subunit 2 (7586-8294)
Mit 9
E01
84659212ATPase, subunits 6-8 (8365-9206)
Mit 10
E02
921210152cytochrome c oxidase, subunit 3 (9207-9990); NADH dehydrogenase, subunit 3 (10059-10404), 5' fragment
Mit 11
F01
996810814NADH dehydrogenase, subunit 3 (10059-10404); NADH dehydrogenase, subunits 4L
Mit 12
F02
1122612198NADH dehydrogenase, subunits 4L-4 (10470-12137)
Mit 13
G01
1218013086NADH dehydrogenase, subunit 5 (12337-14148), 5' fragment
Mit 14
G02
1306714376NADH dehydrogenase, subunit 5 (12337-14148), 3' fragment
Mit 15
H01
1432315450cytochrome b (14747-15887), 5' fragment
Mit 16
H02
1543115932cytochrome b (14747-15887), 3' fragment

CONTROLS

Filter
Description
H03
Plasmid vector pcDNA II, Multiple Cloning Site - PCR product (about 250 bp)
H04
Plasmid vector p0PD, Multiple Cloning Site - PCR product (about 250 bp)
H05
DNA, COT-1, human (Roche); 50-300 bp fragments
H06
DNA, COT-1, human (Roche); 50-300 bp fragments
H07
Arabidopsis thaliana, chromosome 3 genomic DNA (247/268) - PCR product (about 1000 bp)
H08
Arabidopsis thaliana, chromosome 3 genomic DNA (247/269) - PCR product (about 750 bp)
H09
Arabidopsis thaliana, chromosome 3 genomic DNA (33/230) - PCR product (about 1100 bp)
H10
water
H11
water
H12
TE

Show hybridization results
Laveder et al. Supplementary Material