Fungi Diaporthales Diaporthaceae ZhuYaquanMaLeiXueHanLiYongJiangNingNew species of Diaporthe (Diaporthaceae, Diaporthales) from Bauhiniavariegata in ChinaMycoKeys1309202410831733510.3897/mycokeys.108.128983 2A0959D8-088A-5FA5-A7A2-765C956730CF Diaporthe guangzhouensis 854184 Ning Jiang & Y.Q. Zhusp. nov.Fig. 4Etymology.

Named after the collection site of the type specimen, Guangzhou City.

Holotype.

China • Guangdong Province, Guangzhou City, Longdong straight street, 23°11'41.02"N, 113°22'8.33"E, 46 m asl, on diseased leaves of Bauhiniavariegata, 8 Aug 2022, Yong Li, Chengbin Wang & Yaquan Zhu, (holotype: CAF800095; ex-type culture: CFCC 58151).

10.3897/mycokeys.108.128983.figure4B5E70CDE-ED0B-5747-A05F-91B3EFFA11D1

Morphology of DiaportheguangzhouensisA colonies on PDA, MEA and SNA at 25 °C after 2 weeks B the leaf of BauhiniavariegataC conidiomata D conidiogenous cells with attached beta conidia E–G beta conidia. Scale bars: 200 µm (C); 10 µm (D–J).

https://binary.pensoft.net/fig/1132380Description.

Conidiomata pycnidial, scattered to aggregated, black, erumpent, raising above surface of culture medium, subglobose, 150–450 µm diam, exuding white or yellowish creamy conidial droplets from central ostioles after 30 days at 25 °C. Conidiophores 12.5–24.5 × 1–2.5 μm, cylindrical, hyaline, unbranched, straight to sinuous. Conidiogenous cells densely aggregated, phiailidic, unbranched, straight or slightly curved, 5.5–10 × 2.0–7.5 μm. Beta conidia filiform, hyaline, straight or slightly curved, aseptate, 17.0–29.5 × 1.0–2.0 μm. Alpha conidia and gamma conidia not observed. Teleomorph not observed.

Culture characteristics.

Colonies covering entire plate after 2 weeks. On PDA with profuse aerial mycelium, white surface, reverse amber. On MEA with fluffy aerial mycelium, dirty white surface, reverse ochreous. On SNA white sparse aerial mycelium, surface and reverse white.

Additional material examined.

China • Guangdong Province, Guangzhou City, Longdong straight street, 23°11'41.02"N, 113°22'8.33"E, 46 m asl, on diseased leaves of Bauhiniavariegata, 8 Aug 2022, Yong Li, Chengbin Wang & Yaquan Zhu, living culture GZ13E.

Notes.

Diaportheguangzhouensis from the present study is phylogenetically close to D.tulliensis (Fig. 2). Diaportheguangzhouensis differs from D.tulliensis in nucleotide sequence data (5/526 in ITS, 9/347 in tef1, 13/711 in tub2) (Crous et al. 2015). In addition, host and distribution data are vital for species identification (D.guangzhouensis inhabiting Bauhiniavariegata in China vs. D.tulliensis inhabiting Theobromacacao in Australia) (Crous et al. 2015).

10.3897/mycokeys.108.128983.figure2A1B7D625-34C4-5048-AF00-CACCBFAFB06A

Phylogram of Diaporthesojae species complex resulting from a maximum likelihood analysis based on a combined matrix of ITS, cal, his3, tef1 and tub2 loci. Numbers above the branches indicate ML bootstrap values (left, ML BS ≥ 50%) and Bayesian posterior probabilities (right, BPP ≥ 0.9). Isolates from the present study are in bold and ex-type strains are marked with *.

https://binary.pensoft.net/fig/1132378CrousPWWingfieldMJRouxJJRichardsonDMStrasbergDShivasRGAlvaradoPEdwardsJMorenoGSharmaRSonawaneMSTanYPAltésABarasubiyeTBarnesCWBlanchetteRABoertmannDBogoACarlavillaJRCheewangkoonRDanielRde BeerZWYáñez-MoralesMJDuongTAFernández-VicenteJGeeringADWGuestDIHeldBWHeykoopMHubkaVIsmailAMKajaleSCKhemmukWKolaříkMKurliRLebeufRLévesqueCALombardLMagistaDManjónJLMarincowitzSMohedanoJMNovákováAOberliesNHOttoECPaguiganNDPascoeIGPérez-ButrónJLPerroneGRahiPRajaHARintoulTSanhuezaRMVScarlettKShoucheYSShuttleworthLATaylorPWJThornRGVawdreyLLSolano-VidalRVoitkAWongPTWWoodARZamoraJCGroenewaldJZ (2015) Fungal Planet description sheets: 371–399.Persoonia35(1): 264–327. https://doi.org/10.3767/003158515X690269