Synergistic effects of light-emitting probes and peptides for targeting and monitoring integrin expression -- Achilefu et al. 102 (22): 7976 Data Supplement - HTML Page - index.htslp -- Proceedings of the National Academy of Sciences

PNAS Achilefu et al. 10.1073/pnas.0503500102.

Supporting Information

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Supporting Text
Supporting Figure 5
Supporting Figure 6
Supporting Figure 7
Supporting Figure 8
Supporting Figure 9
Supporting Figure 10
Supporting Figure 11
Supporting Table 1

Supporting Figure 5 Fig. 5.
Representative structures of compounds prepared.

Supporting Figure 6 Fig. 6.
Detection of a _v and b ₃ subunits of a _{vb 3}integrase (ABI) in cell lysates and purified integrin protein. Western blot analysis was used to probe for the expression of b ₃ (a) and a _v (b) ABI subunits. The expression level in HeLa cell membrane proteins were not detected. B3I, purified b ₃ integrin lane; AvI, purified a _v integrin; these proteins were used as reference.

Supporting Figure 7 Fig. 7.
Immunostaining of A549 cells.

Supporting Figure 8 Fig. 8.
Measurement of cytotoxicity of cypate-GRD by the MTT assay.

Supporting Figure 9 Fig. 9.
Ex vivo stacked fluorescence images of mitochondrial flavoproteins (a), mitochondrial NADH (b), and Cyp-GRD (c) distribution in A549 tumor tissue at different depths.

Supporting Figure 10

Fig. 10. Three-dimensional fluorescence images of mitochondrial flavoprotein (Fp) (a), NADH (b), NADH/(Fp+NADH) redox ratio (reduced state) (c), and Cyp-GRD (d) in ex vivo A549 tumor tissue 24 h after injection of Cyp-RGD

Supporting Figure 11

Fig. 11. Correlation between mitochondrial NADH expression level and Cyp-GRD distribution in A549 tumor after 24 h after infection at 400 m m (a) and 900 m m (b) scan surface.