Reverse transcription primer,
RT_primer_es15l,/5Phos/TATACACTAAAGATAGGATCCACTAAAGGGGAGGAGGCGTGGGGGG
RT_primer_es27l,/5Phos/TATACACTAAAGATAGGATCCACTAAAAGGACGGGGAGCGGGGGAGA
RT_primer_es27l_tandem,/5Phos/TATACACTAAAGATAGGATCCACTAAAGGGGACCGGCCCGCGGCCCCT
RT_primer_h11,/5Phos/TATACACTAAAGATAGGATCCACTAAAGCTCTTCCCTGTTCACTCGCCGTTACT
RT_primer_es39l,/5Phos/TATACACTAAAGATAGGATCCACTAAAGTGCGGTGCGTGACGGGC
,
Reading probe,
es27l_R_1,/5Phos/GGAGGGAGCGA/3InvdT/
es27l_R_2,/5Phos/GGGAGGGAGCG/3InvdT/
es27l_tandem_R_1,/5Phos/CGAGGAGGAGG/3InvdT/
es27l_tandem_R_2,/5Phos/CCGAGGAGGAG/3InvdT/
h11_ R_1,/5Phos/CTCCGCTGACT/3InvdT/
h11_ R_2,/5Phos/CCTCCGCTGAC/3InvdT/
es15l_R_1,/5Phos/CACCCCCGCCG /3InvdT/
es15l_R_2,/5Phos/ACACCCCCGCC/3InvdT/
es15l_R_3,/5Phos/GACCCCACACC/3InvdT/
es15l_R_4,/5Phos/GGACCCCACAC/3InvdT/
es39l_R_1,/5Phos/CGCGCCCCGTT/3InvdT/
es39l_R_2,/5Phos/CCGCGCCCCGT/3InvdT/
,
TSO and splint ligation probes,
Template switching oligos,rUrArCrCrArACGCrArGrArGrUrACATrGrG+G
Splint ligation primer,/5ACryd/AGTGGATCCTATCTTTAGTGTATATACCAACGCAGAGTACAT
,
Fluorescent decoding oligos,
Decoding_probe_1,/5Alex488N/NNNNNNAA
Decoding_probe_2,/5Alex488N/NNNNNNCC
Decoding_probe_3,/5Alex488N/NNNNNNGG
Decoding_probe_4,/5Alex488N/NNNNNNTT
Decoding_probe_5,/5Alex546N/NNNNNNAC
Decoding_probe_6,/5Alex546N/NNNNNNCA
Decoding_probe_7,/5Alex546N/NNNNNNGT
Decoding_probe_8,/5Alex546N/NNNNNNTG
Decoding_probe_9,/5Alex594N/NNNNNNAG
Decoding_probe_10,/5Alex594N/NNNNNNGA
Decoding_probe_11,/5Alex594N/NNNNNNCT
Decoding_probe_12,/5Alex594N/NNNNNNTC
Decoding_probe_13,/5Alex647N/NNNNNNAT
Decoding_probe_14,/5Alex647N/NNNNNNTA
Decoding_probe_15,/5Alex647N/NNNNNNGC
Decoding_probe_16,/5Alex647N/NNNNNNCG