Table 1. Genes with increased expression under orthotopic conditions as compared with monolayer culture in both U251 and U87 cells

Hs no.*

Gene symbol

Gene description

Regulation of cell cycle

6090

DBC1

Deleted in bladder cancer 1

368078

DNAJA2

DnaJ (Hsp40) homolog, subfamily A, member 2

29353

p25

Brain-specific protein p25

a

283743

PNUTL1

Peanut-like 1 (Drosophila)

14125

SESN1

Sestrin 1

Cell signal transduction

200586

BAI2

Brain-specific angiogenesis inhibitor 2

109798

C6orf48

Chromosome 6 open reading frame 48

282410

CALM1

Calmodulin 1

425808

CALM2

Calmodulin 2

129729

CAPON

C-terminal PDZ domain ligand of neuronal nitric oxide

458426

CCK

Cholecystokinin

355832

CDC42

Cell division cycle 42 (GTP binding protein, 25 kDa)

2281

CHGB

Chromogranin B (secretogranin 1)

150917

CTNNA2

Catenin (cadherin-associated protein),

a 2

410086

CTNNB1

Catenin (cadherin-associated protein),

b 1, 88 kDa

436421

CTNND2

Catenin (cadherin-associated protein),

d 2 (neural)

159311

DKK4

Dickkopf homolog 4 (Xenopus laevis)

381928

DVL3

Dishevelled, dsh homolog 3

30687

GAB2

GRB2-associated binding protein 2

79000

GAP43

Growth-associated protein 43

437710

GAS6

Growth arrest-specific 6

157307

GNAS

GNAS complex locus

232004

NRGN

Neurogranin (protein kinase C substrate, RC3)

232004

NRN1

Neurogranin

154437

PDE2A

Phosphodiesterase 2A, cGMP-stimulated

188

PDE4B

Phosphodiesterase 4B, cAMP-specific

349845

PRKCB1

Protein kinase C,

b 1

155281

PRKCE

Protein kinase C,

e

407181

PRKCZ

PKC

z = protein kinase C, z

387553

PTPN13

Protein tyrosine phosphatase, nonreceptor type 13

156114

PTPNS1

Protein tyrosine phosphatase, nonreceptor type substrate 1

75216

PTPRF

Protein tyrosine phosphatase, receptor type, F

295449

PVALB

Parvalbumin

106185

RALGDS

Ral guanine nucleotide dissociation stimulator

306307

ROCK1

Rho-associated, coiled-coil containing protein kinase 1

296323

SGK

Serum/glucocorticoid-regulated kinase

76930

SNCA

Synuclein,

a (non-A4 component of amyloid precursor)

323833

SNPH

Syntaphilin

435277

SYNGR3

Synaptogyrin 3

401730

SYNJ1

Synaptojanin 1

75667

SYP

Synaptophysin

154679

SYT1

Synaptotagmin I

DNA repair and transcription

150557

BTEB1

Basic transcription element binding protein 1

511952

CBX6

Chromobox homolog 6

211610

CUGBP2

CUG triplet repeat, RNA binding protein 2

326035

EGR1

Early growth response 1

104134

HMX1

Homeo box (H6 family) 1

1569

LHX2

LIM homeobox 2

283063

LMO2

LIM domain only 2 (rhombotin-like 1)

118630

MXI1

MAX interactor 1

410692

PER2

Period homolog 2 (Drosophila)

29117

PURA

Purine-rich element binding protein A

48375

SNRPN

SNRPN upstream reading frame

152207

SSBP2

Single-stranded DNA binding protein 2

210862

TBR1

T-box, brain, 1

114360

TGFB1I4

Transforming growth factor

b 1-induced transcript 4

231840

WBP2

WW domain binding protein 2

446677

ZNF238

Zinc finger protein 238

9568

ZNF261

Zinc finger protein 261

Cell adhesion and surface antigen

199593

AP3B2

Adaptor-related protein complex 3,

b 2 subunit

75196

BAT8

HLA-B associated transcript 8

95327

CD3D

CD3D antigen,

d polypeptide

54457

CD81

CD81 antigen

26126

CLDN10

Claudin 10

6132

CPNE6

Copine VI (neuronal)

48998

FLRT2

Fibronectin leucine-rich transmembrane protein 2

385634

LOC90139

Tetraspanin similar to uroplakin 1

132311

MAP2K1

Mitogen-activated protein kinase kinase 1

25209

MAPK10

Mitogen-activated protein kinase 10

3821

NBEA

Neurobeachin

78792

NCAM1

Neural cell adhesion molecule 1

154729

PDPK1

3-Phosphoinositide-dependent protein kinase-1

275775

SEPP1

Selenoprotein P, plasma, 1

304330

SNAP91

Synaptosomal-associated protein, 91-kDa homolog (mouse)

75671

STX1A

Syntaxin 1A (brain)

325862

STXBP1

Syntaxin binding protein 1

134643

THY1

Thy-1 cell surface antigen

446641

TIMP1

Tissue inhibitor of metalloproteinase 1

Cell structure

378063

APBB1

Amyloid

b (A4) precursor protein binding

194684

BSN

Basson (presynaptic cytomatrix protein)

369579

CAPZA2

Capping protein muscle Z-line,

a 2

41683

CART1

Cartilage paired-class homeoprotein 1

211201

CYFIP2

Cytoplasmic FMR1 interacting protein 2

74617

DCTN1

Dynactin 1

436132

DNM1

Dynamin 1

79226

FEZ1

Fasciculation and elongation protein

z 1

75819

GPM6B

Glycoprotein M6A

2178

HIST2H2BE

Histone 2, H2be

6641

KIF5C

Kinesin family member 5C

101174

MAPT

Microtubule-associated protein

t

280311

MYH10

Myosin, heavy polypeptide 10, nonmuscle

2830

MYOG

Myogenin (myogenic factor 4)

458657

NEF3

Neurofilament 3 (150-kDa medium)

12376

PCLO

Piccolo (presynaptic cytomatrix protein)

80296

PCP4

Purkinje cell protein 4

75445

SPARCL1

SPARC-like 1 (mast9, hevin)

205401

SPTBN1

CDNA: FLJ21418 fis, clone COL04072

512712

TUBB

Tubulin,

b polypeptide

Apotosis

79241

BCL2

B-cell CLL/lymphoma 2

433901

COX8A

Cytochrome c oxidase subunit 8A (ubiquitous)

82548

MADD

Mitogen-associated protein kinase-activating death domain

Oncogene

8004

HAPIP

Huntingtin-associated protein interacting protein (duo)

80205

PIM2

Pim-2 oncogene

78305

RAB2

RAB2, member of RAS oncogene family

8186

TUSC2

Tumor suppressor candidate 2

25292

JUNB

Jun B protooncogene

Receptor

1259

ASGR2

Asialoglycoprotein receptor 2

924

CRYM

Crystallin,

m

80420

CX3CL1

Chemokine (C-X3-C motif) ligand 1

26988

EFNB3

Ephrin-B3

6540

FGF13

Fibroblast growth factor 13

167017

GABBR1

GABA B receptor, 1

175934

GABRA1

GABA A receptor,

a 1

89768

GABRB1

GABA A receptor,

b 1

198612

GPR51

G protein-coupled receptor 51

418105

GRCA

Gene rich cluster, A gene

7117

GRIA1

Glutamate receptor, ionotropic, AMPA 1

335051

GRIA2

Glutamate receptor, ionotropic, AMPA 2

450230

IGFBP3

Insulin-like growth factor binding protein 3

149900

ITPR1

Inositol 1,4,5-triphosphate receptor, type 1

435290

NISCH

Nischarin

82120

NR4A2

Nuclear receptor subfamily 4, group A, member 2

22998

NRXN1

Neurexin 1

431010

RELN

Reelin

12409

SST

Somatostatin

439586

TM4SF2

Transmembrane 4 superfamily member 2

Protein synthesis/degradation

74561

A2M

a

-2-macroglobulin

374638

FKBP1A

FK506 binding protein 1A, 12 kDa

407482

FKBP1B

Hypothetical gene supported by BX647146

117546

NNAT

Neuronatin

434961

SCA1

Spinocerebellar ataxia 1 (olivopontocerebellar ataxia 1, autosomal dominant, ataxin 1)

433680

TG

Thyroglobulin

4552

UBQLN2

Ubiquilin 2

8015

USP21

Ubiquitin-specific protease 21

Transporter/channel

194720

ABCG2

ATP-binding cassette, family G, member 2

389415

CACNA2D2

Calcium channel,

a 2/d subunit 2

417091

CLCN4

Chloride channel 4

408883

SCN1B

Sodium channel, voltage-gated, type I

375616

SLC17A7

Solute carrier family 17

371369

SLC1A3

DKFZP547J0410 protein

2043

SLC25A4

Solute carrier family 25 (mitochondrial carrier)

111967

SLC30A3

Solute carrier family 30 (zinc transporter)

306000

SLC4A1AP

Solute carrier family 4 (anion exchanger)

5462

SLC4A4

Solute carrier family 4

Metabolism

334707

ACY1

Aminoacylase 1

454199

ADCY2

Adenylate cyclase 2

429896

ADH6

Alcohol dehydrogenase 6

435689

ALDH1A2

Aldehyde dehydrogenase 1 family, mamber A2

155247

ALDOC

Aldolase C, fructose-bisphosphate

182215

ARL3

ADP-ribosylation factor-like 3

34114

ATP1A2

ATPase,

a 2 polypeptide

106876

ATP6V0D1

ATPase, lysosomal 38 kDa, V0 subunit d isoform 1

283140

CA10

Carbonic anhydrase X

155097

CA2

Carbonic anhydrase II

173724

CKB

Creatine kinase, brain

155392

CRMP1

Collapsin response mediator protein 1

45127

CSPG5

Neuroglycan C

87417

CTSL2

Cathepsin L2

211201

CYFIP2

Cytoplasmic FMR1 interacting protein 2

152096

CYP2J2

Cytochrome P450, family 2, subfamily J, polypeptide 2

21355

DCAMKL1

Doublecortin and CaM kinase-like 1

277445

DGKZ

Diacylglycerol kinase,

z 104 kDa

511915

ENO2

ENO2=Enolase 2 (gamma, neuronal)

112669

FABP3

Fatty acid binding protein 3, muscle and heart (mammary-derived growth inhibitor)

420036

GAD1

Glutamate decarboxylase 1, brain

86724

GCH1

GTP cyclohydrolase 1

185172

GNB2

Guanine nucleotide binding protein,

b polypeptide 2

435970

GSK3A

Glycogen synthase kinase 3

a

155376

HBB

Hemoglobin,

b

25220

LARGE

Like-glycosyltransferase

234734

LYZ

Lysozyme (renal amyloidosis)

79026

MLF2

Myeloid leukemia factor 2

438737

MT1F

Metallothionein 1F (functional)

21907

MYST2

MYST histone acetyltransferase 2

293007

NPEPPS

Aminopeptidase puromycin sensitive

431279

NSF

N

-ethylmaleimide-sensitive factor

74376

OLFM1

Olfactomedin 1

77318

PAFAH1B1

Platelet-activating factor acetylhydrolase, isoform Ib

57856

PFTK1

PFTAIRE protein kinase 1

277132

PKP4

Plakophilin 4

410477

PLA2R1

Phospholipase A2 receptor 1, 180 kDa

1787

PLP1

Proteolipid protein 1 (Pelizaeus-Merzbacher disease, spastic paraplegia 2, uncomplicated)

183994

PPP1CA

Protein phosphatase 1, catalytic subunit,

a isoform

269777

PPP1R12B

Protein phosphatase 1, regulatory (inhibitor) subunit 12B

272458

PPP3CA

Protein phosphatase 3 (formerly 2B), catalytic subunit,

a isoform

280604

PPP3R1

Protein phosphatase 3 (formerly 2B), regulatory subunit B

283667

RNPEP

Arginyl aminopeptidase (aminopeptidase B)

422181

S100B

S100 calcium binding protein

384598

SERPING1

Serine (or cysteine) proteinase inhibitor

75149

SH3GL2

SH3 domain GRB2-like 2

251754

SLPI

Secretory leukocyte protease inhibitor

48513

SPATA2

Spermatogenesis associated 2

79876

STS

Steroid sulfatase (microsomal)

210862

TBR1

T-box, brain, 1

433923

TF

Transferrin

25001

YWHAG

Tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation

226755

YWHAH

Tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation

*Unigene cluster number, Homo sapiens.