Table 4. Primer sequences used in this study (listed 5'- to -3' end)

Gene or spacer region

Primer sequence (ref.)

  

psbA-trnH

 

psbA3'f

GTTATGCATGAACGTAATGCTC (1)

trnHf

CGCGCATGGTGGATTCACAATCC (2)

rpl36-infA-rps8

 

rpl36f

CACAAATTTTACGAACGAAG (3)

rps8r

TAATGACAGAYCGAGARGCTCGAC (3)

trnK-rps16

 

trnK5'r

TACTCTACCRTTGAGTTAGCAAC (4)

rps16-4547mod

AAAGGKGCTCAACCTACARGAAC (modified 4)

trnV-atpE

 

trnV5f

GTGTAAACGAGTTGCTCTACCA (3)

S1022

CGACATTTGCACATTTAGATGCTAC (5)

trnC-ycf6

 

trnC

CCAGTTCAAATCTGGGTGTC (6)

petN1r

CCCAAGCAAGACTTACTATATCC (7)

ycf6-psbM

 

petN1

GGATATAGTAAGTCTTGCTTGGG (7)

psbM2r

TTCTTGCATTTATTGCTACTGC (7)

psbM-trnD

 

psbM1

GCGGTAGGAACTAGAATAAATAG (7)

trnD

GGGATTGTAGTTCAATTGGT (5)

atpB-rbcL

 

S2r

AGAAGTAGTAGGATTGATTCTCATA (5)

RBCL1

GAATCCAACACTTGCTTTAGTCTCT (5)

trnL-F

 

e

GGTTCAAGTCCCTCTATCCC (8)

f

ATTTGAACTGGTGACACGAG (8)

rbcL

 

1f

ATGTCACCACAAACAGAAAC (9)

724r

TCGCATGTACCTGCAGTAGC (9)

ITS

 

ITS5a

CCTTATCATTTAGAGGAAGGAG (10)

ITS4

TCCTCCGCTTATTGATATGC (11)

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11. White, T. J., Burns, T., Lee, S. & Taylor, J. (1990) in PCR Protocols: A Guide to Methods and Applications, eds. Innis, M., Gelfand, D., Sninsky, J. & White, T. (Academic, San Diego), pp. 315–322.