Name Sequence Use GFP MiSeq REV ACTGGAGTTCAGACGTGTGCTCTTCCGATCttgatgccgttcttctgcttgtcggccatg GFP library prep GFP MiSeq FWD CTCTTTCCCTACACGACGCTCTTCCGATCTtgcttcagccgctaccccgaccacatgaag GFP library prep 1-1 FWD gcggcccgggttcgattcccggccgatgcatcgggcatggcggacttgaagttttagagctagaaatagcaag 24xgRNA GFP 1-1 REV gaccacatgaagcagcacgatgcaccagccgggaatcgaaccc 24xgRNA GFP 1-2 FWD tcgtgctgcttcatgtggtcgttttagagctagaaatagcaag 24xgRNA GFP 1-2 REV aaggctacgtccaggagcgctgcaccagccgggaatcgaaccc 24xgRNA GFP 1-3 FWD gcgctcctggacgtagccttgttttagagctagaaatagcaa 24xgRNA GFP 1-3 REV gggtgtcgccctcgaacttctgcaccagccgggaatcgaaccc 24xgRNA GFP 1-4 FWD gaagttcgagggcgacacccgttttagagctagaaatagcaag 24xgRNA GFP 1-4 REV ccttgaagtcgatgcccttctgcaccagccgggaatcgaaccc 24xgRNA GFP 1-5 FWD gaagggcatcgacttcaagggttttagagctagaaatagcaag 24xgRNA GFP 1-5 REV attttaacttgctatttctagctctaaaacggagcgcaccatcttcttcatgcaccagccgggaatcgaaccc 24xgRNA GFP 2-1 FWD gcggcccgggttcgattcccggccgatgcacatgcccgaaggctacgtccgttttagagctagaaatagcaag 24xgRNA GFP 2-1 REV catgaagcagcacgacttcttgcaccagccgggaatcgaaccc 24xgRNA GFP 2-2 FWD agaagtcgtgctgcttcatggttttagagctagaaatagcaag 24xgRNA GFP 2-2 REV cgccgaggtgaagttcgaggtgcaccagccgggaatcgaaccc 24xgRNA GFP 2-3 FWD cctcgaacttcacctcggcggttttagagctagaaatagcaag 24xgRNA GFP 2-3 REV catcgagctgaagggcatcgtgcaccagccgggaatcgaaccc 24xgRNA GFP 2-4 FWD cgatgcccttcagctcgatggttttagagctagaaatagcaag 24xgRNA GFP 2-4 REV tcctccttgaagtcgatgcctgcaccagccgggaatcgaaccc 24xgRNA GFP 2-5 FWD ggcatcgacttcaaggaggagttttagagctagaaatagcaag 24xgRNA GFP 2-5 REV attttaacttgctatttctagctctaaaactcttcttcaaggacgacggctgcaccagccgggaatcgaaccc 24xgRNA GFP 3-1 FWD gcggcccgggttcgattcccggccgatgcaggagcgcaccatcttcttcagttttagagctagaaatagcaag 24xgRNA GFP 3-1 REV ccatgcccgaaggctacgtctgcaccagccgggaatcgaaccc 24xgRNA GFP 3-2 FWD gacgtagccttcgggcatgggttttagagctagaaatagcaag 24xgRNA GFP 3-2 REV aggtgaagttcgagggcgactgcaccagccgggaatcgaaccc 24xgRNA GFP 3-3 FWD gtcgccctcgaacttcacctgttttagagctagaaatagcaag 24xgRNA GFP 3-3 REV tcagctcgatgcggttcacctgcaccagccgggaatcgaaccc 24xgRNA GFP 3-4 FWD ggtgaaccgcatcgagctgagttttagagctagaaatagcaag 24xgRNA GFP 3-4 REV ggatgttgccgtcctccttgtgcaccagccgggaatcgaaccc 24xgRNA GFP 3-5 FWD caaggaggacggcaacatccgttttagagctagaaatagcaag 24xgRNA GFP 3-5 REV attttaacttgctatttctagctctaaaactcgtccttgaagaagatggttgcaccagccgggaatcgaaccc 24xgRNA GFP 4-1 FWD gcggcccgggttcgattcccggccgatgcacaactacaagacccgcgccggttttagagctagaaatagcaag 24xgRNA GFP 4-1 REV tgcccgaaggctacgtccagtgcaccagccgggaatcgaaccc 24xgRNA GFP 4-2 FWD ctggacgtagccttcgggcagttttagagctagaaatagcaag 24xgRNA GFP 4-2 REV ggtgaaccgcatcgagctgatgcaccagccgggaatcgaaccc 24xgRNA GFP 4-3 FWD tcagctcgatgcggttcaccgttttagagctagaaatagcaag 24xgRNA GFP 4-3 REV tgaagtcgatgcccttcagctgcaccagccgggaatcgaaccc 24xgRNA GFP 4-4 FWD gctgaagggcatcgacttcagttttagagctagaaatagcaag 24xgRNA GFP 4-4 REV aggatgttgccgtcctcctttgcaccagccgggaatcgaaccc 24xgRNA GFP 4-5 FWD aaggaggacggcaacatcctgttttagagctagaaatagcaag 24xgRNA GFP 4-5 REV attttaacttgctatttctagctctaaaaccgaacttcacctcggcgcggtgcaccagccgggaatcgaaccc 24xgRNA GFP 12 FWD tgaagaagatggtgcgctccgttttagagctagaaatagcaag 24xgRNA GFP 12 REV ggacgtagccttcgggcatgtgcaccagccgggaatcgaaccc 24xgRNA GFP 2-1 FWD new catgcccgaaggctacgtccgttttagagctagaaatagcaag 24xgRNA GFP 23 FWD gccgtcgtccttgaagaagagttttagagctagaaatagcaag 24xgRNA GFP 23 REV tgaagaagatggtgcgctcctgcaccagccgggaatcgaaccc 24xgRNA GFP 3-1 FWD new ggagcgcaccatcttcttcagttttagagctagaaatagcaag 24xgRNA GFP 34 FWD accatcttcttcaaggacgagttttagagctagaaatagcaag 24xgRNA GFP 34 REV cggcgcgggtcttgtagttgtgcaccagccgggaatcgaaccc 24xgRNA GFP 4-1 FWD new caactacaagacccgcgccggttttagagctagaaatagcaag 24xgRNA GFP 15 FWD TGCAcctcgaacttcacctcggcg 1xgRNA 20bp 15 REV AAACcgccgaggtgaagttcgagg 1xgRNA 20bp 3 FWD TGCAcgccctcgaacttcacctcggcg 1xgRNA 23bp 3 REV AAACcgccgaggtgaagttcgagggcg 1xgRNA 23bp 8-45 REV AAACtcgggcatggcggacttgaagaagtcgtgctgcttcatgtggtcg gRNAs comparing length 8-45 FWD TGCAcgaccacatgaagcagcacgacttcttcaagtccgccatgcccga gRNAs comparing length 8-30 REV AAACtcgggcatggcggacttgaagaagtcgtgc gRNAs comparing length 8-30 FWD TGCAgcacgacttcttcaagtccgccatgcccga gRNAs comparing length 8-25 REV AAACtcgggcatggcggacttgaagaagt gRNAs comparing length 8-25 FWD TGCAacttcttcaagtccgccatgcccga gRNAs comparing length 8-23 REV AAACtcgggcatggcggacttgaagaa gRNAs comparing length 8-23 FWD TGCAttcttcaagtccgccatgcccga gRNAs comparing length 8-20 REV AAACtcgggcatggcggacttgaa gRNAs comparing length 8-20 FWD TGCAttcaagtccgccatgcccga gRNAs comparing length