Animalia Hydrachnidia Mideopsidae PešićVladimirZawalAndrzejFerreiraSóniaBenitez-BoscoLauraCruz-OliveiraAnaGirãoDinisPadilhaAdrianaTuraccioPaoloRossiniSamanthaBalliniLorenzoStaffoniGiorgiaFratiniSaraCiofiClaudioIannucciAlessioEkremTorbjørnSturElisabethDNA barcode library of Portuguese water mites, with the descriptions of two new species (Acari, Hydrachnidia)Zookeys31102024121711917110.3897/zookeys.1217.131730 AE5CB231-F98D-5E26-8E1A-E4FDB632EDC6 Mideopsis roztoczensis Biesiadka & Kowalik, 1979Fig. 8Material examined.

Portugal, Beja: • Mértola, Moinho de Alferes 1, 37.502°N, 7.69°W, 19 May 2023, leg. Ferreira, Benitez-Bosco, Ekrem, Stur & Turaccio, 1♀ (sequenced); Mértola, Pulo do Lobo, 37.805°N, 7.633°W, 18 May 2023, leg. Ferreira, Benitez-Bosco, Ekrem, Stur & Turaccio, 5♀, 1 deutonymph (sequenced); • Odemira, Ribeira de Seixe, Zambujeira do Mar, 37.398°N, 8.68°W, 75 m a.s.l., 23 May 2023, leg. Ekrem & Benitez-Bosco, 1♂ (sequenced). Bragança • Vinhais, Gasparona, 41.85°N, 7.013°W, 683 m a.s.l., 6 Jul. 2023, leg. Ferreira & Padilha, 1♀, 1 deutonymph (sequenced). Guarda: • Seia, Casa do Loureiro, 40.433°N, 7.701°W, 415 m a.s.l., 19 Jul. 2023 leg. Ferreira & Padilha, 1♂, 3♀, 1 deutonymph (sequenced); • Manteigas, Zêzere, Covão da Ametade, 40.328°N, 7.587°W, 1431 m a.s.l., 21 Aug. 2023, leg. Ferreira, Benitez-Bosco & Padilha, 1♂, 1♀, 1 deutonymph (sequenced); • Manteigas, Zêzere, Ponte dos Frades, 40.403°N, 7.526°W, 672 m a.s.l., 22 Aug. 2023, leg. Ferreira, Benitez-Bosco, Padilha, Andrade & Stur 1♀ (sequenced); • Manteigas, Poço do Inferno, 40.373°N, 7.516°W, 1078 m a.s.l., 21 Aug. 2023, leg. Ferreira, Benitez-Bosco & Padilha, 2♂, 1♀ (juv.), 1 deutonymph, 1♂ dissected and slide mounted (RMNH); • Gouveia, Rio Mondego, Casais de Folgosinho, 40.454°N, 7.493°W, 976 m a.s.l., 24 Aug. 2023, leg. Ferreira, Benitez-Bosco & Padilha, 1♂, 1♀ (sequenced); • Manteigas, Poio do Leão, 40.399°N, 7.541°W, 734 m a.s.l., 22 Aug. 2023, leg. Ferreira, Benitez-Bosco & Padilha, 2♂ (sequenced); • Seia, Rio Alva, Central hidroelétrica de Ponte dos Jugais, river, 40.385°N, 7.706°W, 555 m a.s.l., 23 Aug. 2023, leg. Ferreira, Benitez-Bosco & Padilha, 1♂, 2♀, 2 deutonymph (sequenced), 1♂ dissected and slide mounted (RMNH); • Rio Alva, Nossa Senhora do Desterro, river, 40.395°N, 7.694°W, 791 m a.s.l., 23 Aug. 2023, leg. Ferreira, Benitez-Bosco & Padilha, 2♀ (sequenced); • Manteigas, Mondego, Covão da ponte, 40.443°N, 7.514°W, 999 m a.s.l., 24 Aug. 2023, leg. Ferreira, Benitez-Bosco & Padilha, 1♂ (sequenced); • Seia, Rio Alva, Praia Fluvial de Sabugueiro, river, 40.401°N, 7.64°W, 1021 m a.s.l., 24 Aug. 2023, leg. Ferreira, Benitez-Bosco & Padilha, 2♀ (sequenced); • Manteigas, Casa do Cantoneiro, 40.418°N, 7.603°W, 1378 m a.s.l., 24 Aug. 2023, leg. Ferreira, Benitez-Bosco & Padilha, 2♂, 1♀ (sequenced). Vila Real • Murça, Noura stream, 41.409°N, 7.417°W, 421 m a.s.l., 12 Jul. 2023 leg. Ferreira S, Padilha 1♀ (sequenced).

Remarks.

The specimens examined in our study match the description of Mideopsisroztoczensis, when following Biesiadka and Kowalik (1979). Mideopsisroztoczensis is characterized by a more elevated dorsal shield with distinctly visible anteriorly diverging lines of particularly faint fine porosity, and by the the shape of the male ejaculatory complex with the wedge-shaped anterior ramus being wider and with a characteristic arrow-shaped delimited area. Recently, Pešić et al. (2023e) showed that M.roztoczensis is a genetically variable species, comprising four BINs (BOLD:ACI1492, BOLD:AEN6785, BOLD:AEA2936, BOLD:AEO2944) widely distributed in Europe. In this study, we detected an additional five unique BINs (BOLD:AFU6108, BOLD:AFP5421, BOLD:AFW3785, BOLD:AFV6334, BOLD:AEV2909) within the Portuguese M.roztoczensis-like specimens (Fig. 8), all of them unique, and some of them present at the same sites (e.g., BOLD:AFU6108 and BOLD:AFW3785 in Casa do Loureiro, BOLD:AFU6108 and BOLD:AFV6334 in Casa do Cantoneiro).

10.3897/zookeys.1217.131730.figure83B81EC3E-DC13-5302-8CEB-F8BBBF4BEC8E

Neighbor-Joining tree of the genus Mideopsis obtained from 112 nucleotide COI sequences; 71 sequences were taken from Pešić et al. (2023a) and 41 sequences from Portugal are newly generated in this study. Mundamellagermanica from Montenegro was used as outgroup. BINs are based on the barcode analysis from 16 May 2024. Country codes: BA = Bosnia and Herzegovina, BE = Belgium, CY = Cyprus, DE = Germany, FR = France, ME = Montenegro, PT = Portugal, RS = Serbia, RU = Russia, TR = Turkey. Bootstrap values > 50% from 1000 bootstrap replicates on branches.

https://binary.pensoft.net/fig/1164872
Distribution.

Europe, Turkey. New record for Portugal.

PešićVSmitHKonoplevaES (2023a) Molecular phylogeny and description of Hygrobatescyrnusensis sp. nov. reveals multiple colonization of Corsica by water mites of the H.fluviatilis-complex (Acariformes, Hydrachnidia, Hygrobatidae).Acarologia63(1): 262274. https://doi.org/10.24349/h12f-rs8xBiesiadkaEKowalikW (1979) A new species of Mideopsis Neuman (Hydrachnellae, Acari) from Poland. Bulletin of the Polish Academy of Sciences. Biological Sciences, Cl.II26: 695702.PešićVBańkowskaAZawalARewiczTSmitH (2023e) Molecular diversity and species delimitation in the genus Mideopsis Neuman, 1880 in Europe (Acari, Hydrachnidia, Mideopsidae).Ecologica Montenegrina70: 101115. https://doi.org/10.37828/em.2023.70.12