Table S3: Primers Used in the Study
Barcode amplification (First round PCR)
oAM-R1P-200-F01,TCGTCGGCAGCGTCAGATGTGTATAAGAGACAGNNNNNNNNCTTACTCAGGCCGTAACGTAGGTCTCTGACG
oAM-R1P-201-F01,TCGTCGGCAGCGTCAGATGTGTATAAGAGACAGNNNNNNNNGGACGCATCCGTAACGTAGGTCTCTGACG
oAM-R1P-202-F01,TCGTCGGCAGCGTCAGATGTGTATAAGAGACAGNNNNNNNNAACGTGCCGTAACGTAGGTCTCTGACG
oAM-R1P-203-F01,TCGTCGGCAGCGTCAGATGTGTATAAGAGACAGNNNNNNNNTCGTCCGTAACGTAGGTCTCTGACG
oAM-R1P-204-F01,TCGTCGGCAGCGTCAGATGTGTATAAGAGACAGNNNNNNNNTAGCAGTCGTCCGTAACGTAGGTCTCTGACG
oAM-R1P-205-F01,TCGTCGGCAGCGTCAGATGTGTATAAGAGACAGNNNNNNNNACTTCTAGCCGTAACGTAGGTCTCTGACG
oAM-R1P-206-F01,TCGTCGGCAGCGTCAGATGTGTATAAGAGACAGNNNNNNNNATAGAGTGATACCCGTAACGTAGGTCTCTGACG
oAM-R1P-207-F01,TCGTCGGCAGCGTCAGATGTGTATAAGAGACAGNNNNNNNNCAATTGTGCGCCGTAACGTAGGTCTCTGACG
oAM-R1P-208-F01,TCGTCGGCAGCGTCAGATGTGTATAAGAGACAGNNNNNNNNGCTTACTAGTCCGTAACGTAGGTCTCTGACG
oAM-R1P-209-F01,TCGTCGGCAGCGTCAGATGTGTATAAGAGACAGNNNNNNNNCTGCTTTAACAGCCGTAACGTAGGTCTCTGACG
oAM-R2P-100-R01,GTCTCGTGGGCTCGGAGATGTGTATAAGAGACAGNNNNNNNNGACACAATGCTTGCTGATAAATCTGGAGCC

Gibson Assembly
Tn_BC_Amp_Conn ectorator_2,CAAACTGGACAAAAAAGATCCGTAACGTAGGTCTCTGACG CNNNNCANNNNCANNNNCANNNNCANNNNTTCTACGGGGTCTGACGC
tTEF_to_Tn7R,TTGAACTGAACAAAATAGATCCGTGGATGGCGGCGTTAG