ACT-5 Is an Essential Caenorhabditis elegans Actin Required for Intestinal Microvilli Formation Mol. Biol. Cell MacQueen et al. 16: 3247
Supplemental Figure 1
This article contains the following supporting material:
Supplemental Figure 1 - Figure 1. A truncated ACT-5 polypeptide accumulates in animals that carry the act-5(dt2107) mutation. Western blots of total SDS-soluble worm protein extracts from wildtype (Lanes 1-3), act-5(dt2017) (Lane 4) or act-5(dt2017dt2019) (Lane 5) strains. The two mutant strains are rescued by an integrated copy of the act-5(+) gene. Lane 1 probed with a pan-reactive anti-actin monoclonal antibody C4 that recognizes all worm actins (arrow, 43 kDa). Lanes 2-5 probed with affinity-purified anti-ACT-5 antibodies. The alkaline phosphatase detection was allowed to develop for approximately 1 minute (Lanes 1 and 2) or for 10 minutes (Lanes 3-5) to better visualize the low abundance ~38 kDa truncation product specific to the act-5(dt2017) extract (Lane 4, arrowhead). Each lane contains 7 mg of worm extract.