Table 3. Cytokinin levels in transgenic Arabidopsis lines harboring pTA-7001
Cytokinin level, pmol · g- 1 fresh weight | |||||||||||||||||||
+ DEX | - DEX | ||||||||||||||||||
0 h | 6 h | 12 h | 6 h | 12 h | |||||||||||||||
tZ | 0.29 | ± | 0.02 | 0.31 | ± | 0.02 | 0.42 | ± | 0.01 | 0.28 | ± | 0.03 | 0.36 | ± | 0.03 | ||||
tZR | 1.01 | ± | 0.07 | 1.19 | ± | 0.11 | 2.22 | ± | 0.20 | 1.12 | ± | 0.12 | 1.42 | ± | 0.20 | ||||
tZRMP | 3.16 | ± | 0.20 | 3.21 | ± | 0.41 | 5.43 | ± | 0.29 | 2.63 | ± | 0.43 | 3.78 | ± | 0.31 | ||||
cZ | 0.19 | ± | 0.02 | 0.14 | ± | 0.00 | 0.12 | ± | 0.01 | 0.16 | ± | 0.00 | 0.20 | ± | 0.01 | ||||
cZR | 0.38 | ± | 0.05 | 0.17 | ± | 0.01 | 0.18 | ± | 0.03 | 0.22 | ± | 0.02 | 0.38 | ± | 0.05 | ||||
cZRMP | 0.90 | ± | 0.18 | 0.30 | ± | 0.02 | 0.18 | ± | 0.04 | 0.54 | ± | 0.05 | 0.72 | ± | 0.17 | ||||
DZ | ND | ND | ND | ND | ND | ||||||||||||||
DZR | 0.01 | ± | 0.00 | 0.01 | ± | 0.00 | 0.02 | ± | 0.00 | 0.01 | ± | 0.00 | 0.01 | ± | 0.00 | ||||
DZRMP | 0.03 | ± | 0.00 | 0.03 | ± | 0.01 | 0.06 | ± | 0.01 | 0.03 | ± | 0.00 | 0.03 | ± | 0.00 | ||||
iP | 0.37 | ± | 0.01 | 0.37 | ± | 0.03 | 0.35 | ± | 0.02 | 0.36 | ± | 0.01 | 0.37 | ± | 0.02 | ||||
iPR | 1.77 | ± | 0.38 | 2.10 | ± | 0.50 | 1.76 | ± | 0.39 | 2.28 | ± | 0.24 | 1.86 | ± | 0.52 | ||||
iPRMP | 17.10 | ± | 0.89 | 19.35 | ± | 1.74 | 17.81 | ± | 1.66 | 21.17 | ± | 3.04 | 18.49 | ± | 4.20 | ||||
tZ7G | 13.64 | ± | 0.51 | 12.24 | ± | 0.28 | 11.22 | ± | 0.15 | 11.89 | ± | 0.29 | 12.16 | ± | 0.34 | ||||
tZOG | 4.93 | ± | 0.49 | 4.55 | ± | 0.08 | 4.44 | ± | 0.06 | 5.08 | ± | 0.26 | 4.86 | ± | 0.43 | ||||
tZ9G | 6.54 | ± | 0.20 | 6.13 | ± | 0.09 | 5.58 | ± | 0.04 | 5.66 | ± | 0.24 | 5.88 | ± | 0.08 | ||||
tZROG | 0.31 | ± | 0.03 | 0.28 | ± | 0.00 | 0.25 | ± | 0.02 | 0.27 | ± | 0.03 | 0.28 | ± | 0.02 | ||||
DZ9G | 0.07 | ± | 0.01 | 0.06 | ± | 0.01 | 0.06 | ± | 0.00 | 0.05 | ± | 0.00 | 0.06 | ± | 0.00 | ||||
iP7G | 18.85 | ± | 0.85 | 16.83 | ± | 1.20 | 16.44 | ± | 0.39 | 18.35 | ± | 0.56 | 17.09 | ± | 0.32 | ||||
iP9G | 1.26 | ± | 0.03 | 1.13 | ± | 0.05 | 1.08 | ± | 0.01 | 1.19 | ± | 0.02 | 1.12 | ± | 0.00 | ||||
Transgenic Arabidopsis seedlings harboring pTA7001 were grown for 18 days on MGRL-agar plates, and then subjected to dexamethasone (+DEX) or mock (-DEX) treatment. After 0, 6, and 12 h, the seedlings were harvested, and CK contents were measured. tZ, trans-zeatin; tZR, tZ riboside; tZRMP, tZR 5'-monophosphate; cZ, cis-zeatin; cZR, cZ riboside; cZRMP, cZR 5'-monophosphate; DZ, dihydrozeatin; DZR, DZ riboside; DZRMP, DZR 5'-monophosphate; iP, isopentenyladenine; iPR, iP riboside; iPRMP, iPR 5'-monophosphate; tZ7G, tZ-7-N-glucoside; tZOG, tZ-O-glucoside; tZ9G, tZ-9-N-glucoside; tZROG, tZR-O-glucoside; DZ9G, DZ-9-N-glucoside; iP7G, iP-7-N-glucoside; iP9G, iP-9-N-glucoside. Means ± SD (n = 3). ND, not detected.