Lolli et al. 10.1073/pnas.0503178102. |
Table 3.
Analytical data of the synthesized peptidesPeptide | R t, min | HPLC method | ESI-MS | Yield, mg/% |
[Asn31(Glc)]hMOG(30-50) (1) | 18.8 | 30-50% B in 30 min | 1304.2 (2605.3) | 3.0/31 |
hMOG(30-50) (1) | 19.2 | 35-50% B in 30 min | 1223.1 (2443.3) | 3.1/40 |
[Asn84(Glc)]hMBP(83-99) (2) | 18.1 | 25-50% B in 30 min | 1079.6 (2156.1) | 2.5/28 |
hMBP(83-99) (2) | 17.7 | 25-50% B in 30 min | 998.5 (1994.1) | 2.5/30 |
CSF114(Glc) (3) | 14.2 | 30% B* | 1304.2 (2605.3) | 2000/30 |
CSF114 (3) | 6.2 | 21-42% B in 30 min | 1223.1 (2443.3) | 5.0/33 |
CSF114(GlcAc4) (4) | 13.2 | 30-50% B in 30 min | 926.0 (2773.4) | 2.0/29 |
CSF114(GlcNAc) (5) | 15.8 | 25-50% B in 30 min | 1325.3 (2646.3) | 10/37 |
CSF114(Glcb4Glc) (6) | 8.5 | 20-60% B in 20 min | 1384.3 (2767.4) | 1.5/23 |
[Ser7(Glc)]CSF114 (7) | 12.0 | 20-40% B in 30 min | 1290.0 (2578.3) | 3.7/32 |
MBH36(Glc) (8) | 9.0 | 15-40% B in 30 min | 1840.1 (1838.9) | 2.5/33 |
MBH36 (8) | 16.1 | 10-40% B in 30 min | 1678.0 (1676.8) | 3.2/40 |
[Thr9]CSF114(Glc) (9) | 9.0 | 25-30% B in 30 min | 1285.8 (2569.3) | 2.1/28 |
[Thr9]CSF114 (9) | 17.1 | 25-50% B in 30 min | 1205.1 (2407.2) | 2.7/39 |
Ac-[c(Dap5,Asp10)]CSF114(Glc) (10) | 16.7 | 28% B* | 873.2 (2614.3) | 1.5/29 |
Ac-[c(Dap5,Asp10)]CSF114 (10) | 8.7 | 28% B* | 1227.8 (2452.3) | 1.7/30 |
Scramble CSF114(Glc) (11) | 7.0 | 28% B* | 1304.1 (2605.3) | 3.2/29 |
Scramble CSF114 (11) | 15.7 | 28% B* | 1223.2 (2443.3) | 4.3/33 |
Electrospray ionization (ESI) mass spectroscopy (MS) values are shown as the statistically most frequent monoisotopic mass, M. These values were determined as [M+2H]2+ unless otherwise noted (, determined as [M+H]+; and , determined as [M+3H]3+). Yields were obtained after purification by semi-preparative RP-HPLC (purity >98%). *, HPLC elution was isocratic.