Lolli et al. 10.1073/pnas.0503178102.

Supporting Information

Files in this Data Supplement:

Supporting Materials and Methods
Supporting Table 3




Table 3.

Analytical data of the synthesized peptides

Peptide

R

t, min

HPLC method

ESI-MS
found (calc.)*

Yield, mg/%

[Asn31(Glc)]hMOG(30-50) (1)

18.8

30-50% B in 30 min

1304.2 (2605.3)

3.0/31

hMOG(30-50) (1’)

19.2

35-50% B in 30 min

1223.1 (2443.3)

3.1/40

[Asn84(Glc)]hMBP(83-99) (2)

18.1

25-50% B in 30 min

1079.6 (2156.1)

2.5/28

hMBP(83-99) (2’)

17.7

25-50% B in 30 min

998.5 (1994.1)

2.5/30

CSF114(Glc) (3)

14.2

30% B*

1304.2 (2605.3)

2000/30

CSF114 (3’)

6.2

21-42% B in 30 min

1223.1 (2443.3)

5.0/33

CSF114(GlcAc4) (4)

13.2

30-50% B in 30 min

926.0 (2773.4)

2.0/29

CSF114(GlcNAc) (5)

15.8

25-50% B in 30 min

1325.3 (2646.3)

10/37

CSF114(Glcb4Glc) (6)

8.5

20-60% B in 20 min

1384.3 (2767.4)

1.5/23

[Ser7(Glc)]CSF114 (7)

12.0

20-40% B in 30 min

1290.0 (2578.3)

3.7/32

MBH36(Glc) (8)

9.0

15-40% B in 30 min

1840.1 (1838.9)

2.5/33

MBH36 (8’)

16.1

10-40% B in 30 min

1678.0 (1676.8)

3.2/40

[Thr9]CSF114(Glc) (9)

9.0

25-30% B in 30 min

1285.8 (2569.3)

2.1/28

[Thr9]CSF114 (9’)

17.1

25-50% B in 30 min

1205.1 (2407.2)

2.7/39

Ac-[c(Dap5,Asp10)]CSF114(Glc) (10)

16.7

28% B*

873.2 (2614.3)

1.5/29

Ac-[c(Dap5,Asp10)]CSF114 (10’)

8.7

28% B*

1227.8 (2452.3)

1.7/30

Scramble CSF114(Glc) (11)

7.0

28% B*

1304.1 (2605.3)

3.2/29

Scramble CSF114 (11’)

15.7

28% B*

1223.2 (2443.3)

4.3/33

Electrospray ionization (ESI) mass spectroscopy (MS) values are shown as the statistically most frequent monoisotopic mass, M. These values were determined as [M+2H]2+ unless otherwise noted (†, determined as [M+H]+; and ‡, determined as [M+3H]3+). Yields were obtained after purification by semi-preparative RP-HPLC (purity >98%). *, HPLC elution was isocratic.