Fraga et al. 10.1073/pnas.0500398102. |
Supporting Table 2
Supporting Table 3
Supporting Figure 5
Supporting Table 4
Supporting Table 5
Supporting Figure 6
Supporting Figure 5
Fig. 5. Temporal fluctuation of epigenetic variables in normal lymphocytes obtained from MZ twins. 5mC, 5-methyl-cytosine; AcH4, histone H4 acetylation; AcH3, histone H3 acetylation and methylation of the Alu-Sp. (A) One year monitoring of epigenetic values in a 40-year-old twin pair. (B) Comparison of epigenetic variability (measured by the Euclidean squared distance, ESD) between 19-, 23-, 30-, 44-, 48-, and 66-year-old MZ twin pairs in a 12-week time period. Results are expressed as mean; bars, ±SD.
Fig. 6.
Epigenetic disparities in skeletal muscle and epithelial mouth cells of MZ twins. (A) Bisulfite genomic sequencing of 12 clones of the genomic locus PKD1P2 obtained by AIMS in skeletal muscle of 28- and 64-year-old twin pairs. (Upper) Schematic representations of the methylation status of each CpG dinucleotide. Black and white dots indicate methylated and unmethylated CpGs, respectively. (Lower) Sequencing reactions of bisulfite-modified DNA. Two representative electropherograms showing sequence-specific DNA methylation changes at the PKD1P2 sequence in 64-year-old twin pairs are shown. (B) Example of an AIMS analysis in 19- and 64-year-old twin pairs. Differential anonymous bands corresponding to sibling-specific changes of DNA methylation between 50-year-old twins are indicated with arrows. (C) Measurement of epigenetic variables 5-methylcytosine (5mC), histone H4 acetylation (AcH4), histone H3 acetylation (AcH3), and methylation of the Alu-Sp in epithelial mouth cells obtained from 19-, 23-, 30-, and 44-year-old MZ twin pairs.