Supplemental Materials

This article contains the following supporting material:

• Supplemental Figure 1 - Loss of basal bodies is due to the absence of Cen1. (A) Two cen1Δ cells approaching cell division 48 hours after the parent strains had been mated. Cells were fixed and stained with the polyclonal antiCen1 antibody (Green), and DAPI to visualize DNA (Blue). The example on the left reveals problems in assembling a new oral apparatus and the increasing disorder of basal bodies in the posterior half of the cell. The example on the right also indicates that nuclear divisions seem to be able to progress normally (both the micronucleus and the macronucleus have divided). (B) Two cells from a culture resulting from the mixing of cen1Δ knockout heterokaryon strains UCB8 and UCB9 without the presence of paromomycin. The cell on the left demonstrates the cen1Δ phenotype, while the cell on the right is likely a normal, parental cell. Cells were fixed and stained with the 20H5 monoclonal centrin antibody. (C) A wild-type cell (strain CU428) exposed to paromomycin for 72 hours, fixed and stained with the polyclonal antiCen1 antibody. Scale bars = 10μm.
• Supplemental Table 1