Vitamin C biosynthesis in trypanosomes: A role for the glycosome -- Wilkinson et al. 102 (33): 11645 Data Supplement - HTML Page - index.htslp -- Proceedings of the National Academy of Sciences

Wilkinson et al. 10.1073/pnas.0504251102.

Supporting Table

Table 2. Oligonucleotides used in this study

	Primer	Sequence (5' to 3')
Protein expression	TbALO4	ggatccAGGAGACGATGTCCGATGGG
Protein expression	TbALO5	gaattcCTACTACAGATGACTAATAGT
Gene deletion 5' flank	TbALO8	gagctcCCGCGGTGAGTATCTCCTAATGGCGATTT
Gene deletion 5' flank	TbALO9	tctagaGGATCCATACAAAAGGTTGCTATGAATGGA
3' flank	TbALO10	gggcccTAGCGGACGTAACAGATCATA
3' flank	TbALO11	ggtaccTTGCAGCTAGTGACTAAGGAC
Localization	TbALO7	ggatccCTACTACAGATGACTAATAGT
Localization	TbALO12	tctagaGATGGGACTTGGACTAACTTT

The sequences in lower case correspond to restriction sites incorporated into the primers to facilitate cloning.