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. 2005 Aug;25(15):6834–6845. doi: 10.1128/MCB.25.15.6834-6845.2005

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Copyright © 2005, American Society for Microbiology

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FIG. 3. — Macroscopic and microscopic analyses of AF5q31-deficient mice at different ages and the expression profiles of AF5q31 in the normal mouse embryos. (A) Gross morphology of neonatal littermates representing AF5q31^+/+ (right), AF5q31^+/− (center), and AF5q31^−/− (left). (B and C) Histological sections of the lung from AF5q31^+/+ (B) and AF5q31^−/− (C) neonatal littermates stained with hematoxylin and eosin stain. (D) Gross morphology of the AF5q31^+/+, AF5q31^+/−, and AF5q31^−/− embryos of a litter at 10.5 dpc. (E) Northern blot analysis of total RNAs (20 μg/lane) from each embryo stage of the wild-type mouse. The blot was hybridized to a radioactive AF5q31 probe. As a loading control, 18S and 28S rRNAs in total RNA are demonstrated.