APPENDIX 1							
Exon	Direction	Sequence	Annealing temperature	Fragment size	Notes		
1	Forward	CGTGTATAGTGATCAGATAAGG	62	370			
	Reverse	CTTAAGTGTGCGCTGTTGGTC					
2	Forward	CCTTGTCTGGCATTTGTCAGG	62	512	25% 7-deaza-dGTP needed to amplify		
	Reverse	CTTGCTCCAGGAGATGCTTG					
3	Forward	GCCAAGAGACCATATCGAGG	62	410			
	Reverse	GATACATCCCACCTGCTCC					
4	Forward	GCTGCACCTTGGAATCTACC	62	225			
	Reverse	CCTAGACTCTCCTGAGCCAC					
5	Forward	AAGGCCACTATGCTTGGAGC	62	400			
	Reverse	GACCCAAGTGTGGTCAGAAC					
6/7	Forward	CATGTGCAAGGACTTCATGG	62	600			
	Reverse	AACCACCTCCGACCACTGC					
8	Forward	GATCCTGTTCTTACTAACGATG	62	320			
	Reverse	AGTTCCTTCAGCACTGTCATC					
9	Forward	GACAGGTTTGGGCCTCGATC	62	240			
	Reverse	AGACACACGTGGCAGGCTG					
10/11	Forward	TACTGAGAGAGGTTTAGCCTG	62	518			
	Reverse	CCAAATGCCAGGGAGCTCAG					
12	Forward	CCATCCTGCTGTCACTTGG	62	326			
	Reverse	GTTCAGTCTCCAAGCCTCTC					
13	Forward	GCCAGCTCAATGGATGGATC	62	360			
	Reverse	AGCTCATTTCACCAGCCTTTC					
14/15	Forward	GAAGTCTGGAGAGTGCAGATG	62	509			
	Reverse	GGAGGCTAAGGGTGTGCTG					
1-3	Forward	TGTACGTGTATAGTGATCAGATAAGGG	76	27			
1-3	Reverse	AGCTGGATACATCCCACCTGCTCC	76	24			
6-15	Forward	CGCCATGTGCAAGGACTTCATGGG	76	24			
6-15	Reverse	CTGGAGGCTAAGGGTGTGCTGGC	76	23			
							
Allele-specific oligonucleotides and restriction digests							
Allele		
A167V	A167	TCAGCTGCTTATAGG
	V167	TCAGCTGTGGAGAGG
D426N	D426	GACCCTGGACGTGGA
	N426	GACCCTGAACGTGGA
Q521E	Q521	creates a Sex A1 site
	E521	creates a Bss S1 site
Q521R	R521	creates a Msp I site