Supporting information for McKinney-Freeman et al. (2002) Proc. Natl. Acad. Sci. USA 99 (3), 1341–1346. (10.1073/pnas.032438799)
Fig. 6.
Muscle-derived myeloid engraftment is less efficient than WBM-derived myeloid engraftment. PB of animals transplanted with Sca-1+/CD45+ muscle-derived cells was analyzed at the indicated time points after transplant. Myeloid engraftment was measured by using a combination of two monoclonal antibodies that recognize granulocytes (Gr.1) and macrophages (Mac-1). Percent myeloid cells shown here is the fraction of myeloid cells that were derived from muscle (white bars) or bone marrow (black bars) divided by the total engraftment contributed by the indicated cell population. Percent muscle-derived myeloid contribution was compared to percent WBM competitor myeloid engraftment. A paired two-sided Student’s t-test was used to assess statistical significance. Because short-term multipotent progenitors have also been found to behave similarly at late time points after transplant, these data imply that the muscle-hematopoietic stem cell (ms-HSC) population may contain short-term multipotent progenitors with the potential for only limited self renewal. Alternatively, there could be some skewing in contribution when relatively low levels of engraftment are achieved, as in these experiments. Finally, perhaps ms-HSCs are distinct from WBM-derived HSCs in their ability to reconstitute the myeloid compartment.