Supporting information for Wu et al. (2002) Proc. Natl. Acad. Sci. USA 99 (4), 23922397. (10.1073/pnas.042617699)
Table 1. Sequence of oligonucleotide primers used to detect Ga t, Ggust, and T2R expression in rat and mouse
Forward | Reverse | Size, bp | |
Gt-1(rod) | AACCGAATGCACGAGAGCCT | AGAGCCCGCAGTCTTTGAGG | 340 |
Gt-2(cone) | AATCGCATGCACGAGTCTTTG | AGAGCCCACAGTCCTTGAGG | 340 |
Ggust | AGTCTTCACCTCTTCAACAG | GCTCAGAAGAGCCCTGTGTC | 332 |
rT2R1/mT2R19 | GATCTGCTTCTTTTTTGCCTG | AAAATTAAGACAATCGAGTGT | 698 |
rT2R2 | GATCAGATTCTCACTGCTTTG | CAGAATCAGCACAAATGAGTG | 768 |
rT2R3 | GAAATAATTCTCATCAGCCTG | CATCATCAGGGAAGTGAAGTG | 705 |
rT2R4 | CATTGGCTTGGCGATTTCCAG | TAGAATTAAGATGATTGAGTG | 697 |
rT2R5 | CACTGGCTTGGCAATTTCCAG | TAGAATTAGGATAAGTGAGTG | 700 |
rT2R6* | CTCAAGTCTGGCTATGTCCAG | AAGGATCAGGATAAATGAATG | 718 |
rT2R7 | GCTTTTCTTGGCCATTTCCAG | CAAAATCAAGATAAATGAGTG | 706 |
rT2R8 | TCTTACATTGGCAATTTCAAG | TAGAATCAGGATAAATGAGTG | 697 |
rT2R9 | CTTTGGCTTAGCAACTTCCAG | TAGAATTAGAATGACTGAGTG | 700 |
rT2R10* | atgttcttacacacaataaag | ACTCTCCACTTTAGGTAAAGA | 392 |
rT2R12 | GACATGATCCTCCTCAGTTTG | AGTGATAAGGATAAATGGATG | 726 |
mT2R5 | CATCGGCTTAGCAACTTCCAG | TAGAATTAGAATAAATGAGTG | 700 |
mT2R8 | GATAGGATCCTGTTCAGCTTG | ATAATGAGGAGGACAGAATGT | 707 |
*Partial sequence.