Supporting information for Wu et al. (2002) Proc. Natl. Acad. Sci. USA 99 (4), 2392–2397. (10.1073/pnas.042617699)

Table 1. Sequence of oligonucleotide primers used to detect Ga t, Ggust, and T2R expression in rat and mouse

 

Forward

Reverse

Size, bp

Gt-1(rod)

AACCGAATGCACGAGAGCCT

AGAGCCCGCAGTCTTTGAGG

340

Gt-2(cone)

AATCGCATGCACGAGTCTTTG

AGAGCCCACAGTCCTTGAGG

340

Ggust

AGTCTTCACCTCTTCAACAG

GCTCAGAAGAGCCCTGTGTC

332

rT2R1/mT2R19

GATCTGCTTCTTTTTTGCCTG

AAAATTAAGACAATCGAGTGT

698

rT2R2

GATCAGATTCTCACTGCTTTG

CAGAATCAGCACAAATGAGTG

768

rT2R3

GAAATAATTCTCATCAGCCTG

CATCATCAGGGAAGTGAAGTG

705

rT2R4

CATTGGCTTGGCGATTTCCAG

TAGAATTAAGATGATTGAGTG

697

rT2R5

CACTGGCTTGGCAATTTCCAG

TAGAATTAGGATAAGTGAGTG

700

rT2R6*

CTCAAGTCTGGCTATGTCCAG

AAGGATCAGGATAAATGAATG

718

rT2R7

GCTTTTCTTGGCCATTTCCAG

CAAAATCAAGATAAATGAGTG

706

rT2R8

TCTTACATTGGCAATTTCAAG

TAGAATCAGGATAAATGAGTG

697

rT2R9

CTTTGGCTTAGCAACTTCCAG

TAGAATTAGAATGACTGAGTG

700

rT2R10*

atgttcttacacacaataaag

ACTCTCCACTTTAGGTAAAGA

392

rT2R12

GACATGATCCTCCTCAGTTTG

AGTGATAAGGATAAATGGATG

726

mT2R5

CATCGGCTTAGCAACTTCCAG

TAGAATTAGAATAAATGAGTG

700


mT2R8

GATAGGATCCTGTTCAGCTTG

ATAATGAGGAGGACAGAATGT

707

*Partial sequence.