Complex pattern of Mycobacterium marinum gene expression during long-term granulomatous infection -- Data Supplement - Supporting Figure 5 -- Proceedings of the National Academy of Sciences

Supporting information for Chan et al. (March 12, 2002) Proc. Natl. Acad. Sci. USA, 10.1073/pnas.002024599.

Fig, 5.
Growth of the L1D mutant in low pH medium and in J774 macrophages. (A) Cultures of wild-type and L1D mutant M. marinum were diluted into OADC-Tw medium at pH 7.0 (solid lines) and pH 6.25 (dotted lines), respectively, as described in the text. Solid symbols and open symbols represent wild-type and L1D mutant counts, respectively. Day 1 is the time point immediately after dilution of the cultures into the assay medium. (B) Growth of the two strains in J774 cells as judged by counting viable bacteria after lysing the cells. A total of 1.2 ´ 10⁵ wild-type or 2.4 ´ 10⁵ L1D mutant bacteria was used to infect each well. Day 1 is the time point 5 h postinfection. Solid and open squares represent wild-type and mutant bacteria, respectively. Error bars represent the standard errors of the means for triplicate samples in both A and B .