Supporting information for Meuer et al. (April 2, 2002) Proc. Natl. Acad. Sci. USA, 10.1073/pnas.072615499.

Table 5. PCR primers used in the study

Primer name

Sequence

Restriction sites*

ech

-for

GGGGCGCGCCTAGGTGGGTCTCAGGAGCAATACAAAAG

Spe

I

ech

-rev

GGGGCGCGCCTAGGCCTACTGTATCCACACTTACCG

Spe

I

ech

'-for

GGGGCGCGCCTAGGTGGGTCTCAGGAGCAATACAAAAG

Avr

II

ech

'-rev

GGGGGATCCATATGGCGTATCCTCCGATCTATTAATCC

Bam

HI

'ech-for

GGGGGATCCGGACTATGTCAAAAGAATGCC

Bam

HI

'ech-rev

GGGGCGCGCCTAGGCCTACTGTATCCACACTTACCG

Avr

II

hpt

-for

GAATTCGGCGCGCCCCAGGAATGGCAGCTGAAGG

Asc

I

hpt

-rev

GAATTCGGCGCGCCTCGTTCCCACAAGCTCTCCA

Asc

I

uidA-

for

GGGGGGCATATGTTACGTCCTGTAGAAACCC

Nde

I

uidA-

rev

GGGGGGAGATCTGATCATTAACGGCGCAGTACCG

Bgl

II

ech

p-for

GCCGCCGCATGCGAGCTCATGTCCGTTACTGCCGTTTC

Sph

I

ech

p-rev

GCCGCCGCCCATATGGCGTATCCTCCGATCTATTAATCC

Nde

I

*Restriction sites added to each primer are shown in bold in the sequence.