A monomeric red fluorescent protein -- Data Supplement - Supporting Information -- Proceedings of the National Academy of Sciences

Supporting information for Campbell et al. (2002) Proc. Natl. Acad. Sci. USA 99 (12), 7877–7882. (10.1073/pnas.082243699)

Primers for general use

PRSET_BamH1_FW

GTA CGA CGA TGA CGA TAA GGA TCC

PRSET_EcoR1_RV

GCA GCC GGA TCA AGC TTC GAA TTC

Primers used to construct libraries for DsRed dimer evolution

N42X_V44X_FW

CGC CCC TAC GAG GGC CAC MWS ACC VYC AAG CTG AAG GTG ACC AAGG

N42X_V44X_RV

CCT TGG TCA CCT TCA GCT TGR BGG TSW KGT GGC CCT CGT AGG GGC G

K70X_V71X_FW

CAG TTC CAG TAC GGC TCC NNK NYC TAC GTG AAG CAC CCC GCC

K70X_V71X_RV

GGC GGG GTG CTT CAC GTA GRN MNN GGA GCC GTA CTG GAA CTG

C117X_V127T_FW

CAG GAC GGC RVS NTS ATC TAC AAG GTG AAG NTS CGC GGC ACC AAC TTC

C117X_V127T_RV

GAA GTT GGT GCC GCG SAN CTT CAC CTT GTA GAT SAN SBY GCC GTC CTG

I161X_K163X_FW

GGC GTG CTG AAG GGC GAG VYC CAC MWS GCC CTG AAG CTG AAG GACG

I161X_K163X_RV

CGT CCT TCA GCT TCA GGG CSW KGT GGR BCT CGC CCT TCA GCA CGC C

S179X_FW

GGT GGA GTT CAA GNC CAT CTA CAT GGC CAA G

S179X_RV

CTT GGC CAT GTA GAT GGN CTT GAA CTC CAC C

S197X_A_FW

CTA CTA CTA CGT GGA CNC CAA GCT GGA CAT CAC C

S197X_A_RV

P-GGT GAT GTC CAG CTT GKN GTC CAC GTA GTA GTA G

S197_B_FW

C TAC TAC TAC GTG GAC NNK AAG CTG GAC ATC ACC

S197_B_RV

GGT GAT GTC CAG CTT MNN GTC CAC GTA GTA GTA G

T217X_FW

CAG TAC GAG CGC NCC GAG GGC CGC CAC

T217X_RV

GTG GCG GCC CTC GGN GCG CTC GTA CTG

Primers used for DsRed monomer evolution

K70X_K83X_FW

AAG CAC CCC GCC GAC ATC CCC GAC TAC WWK AAG CTG TCC

K70X_K83X_RV

GAT GTC GGC GGG GTG CTT CAC GTA GRC CYT GGA GCC GTA CTG

C117X_S131X_FW

GTG AAG NTS CGC GGC ACC AAC TTC CCC YCC GAC GGC CCC

C117X_S131X_RV

GAA GTT GGT GCC GCG SAN CTT CAC CTT GTA GAT GAA SBY GCC GTC CTG

E144X_A164X_FW

CTG TAC CCC CGC GAC GGC GTG CTG AAG GGC GAG NHC ARG NWS ARG CTG AAG CTG AAG GAC GGC GGC

E144X_A164X_RV

CTT CAG CAC GCC GTC GCG GGG GTA CAG GCG CTC GGT GST SBB SBB CCA GCC CAT RGT CTT CTT CTG

A145X_V156X_FW

TCC ACC GAG CRS NTS TAC CCC VAS GAC GGC RBC CTG AAG GGC

A145X_V156X_RV

GCC GTC STB GGG GTA SAN SYG CTC GGT GGA GGS CTC CCA GCC

H162X_A164X_FW

CTG AAG GGC GAG ATC ARG MWS ARG CTG AAG CTG AAG GAC

H162X_A164X_RV

GTC CTT CAG CTT CAG CYT SWK CYT GAT CTC GCC CTT CAG

L174X_I180X_FW

GGC CAC TAC VVC NYC GAG NYC AAG ACC AYC TAC ATG GCC

L174X_I180X_RV

GGC CAT GTA GRT GGT CTT GRN CTC GRN GBB GTA GTG GCC

Y192A_Y194A_FW

GTG CAG CTG CCC GGC GCC TAC GCC GTG GAC ACC AAG CTG

Y192A_Y194A_RV

CAG CTT GGT GTC CAC GGC GTA GGC GCC GGG CAG CTG CAC

Y192X_S197X_FW

GGC RMS TAC RMS RYC GAC RYC AAG CTG GAC ATC ACC

Y192X _S197X_RV

CTT GRY GTC GRY SKY GTA SKY GCC GGG CAG CTG CAC

T217X_L225X_RV

c gaa ttc tta sky gcc sky gcc gtg gcg gcc ctc ggh gcg ctc

H222G_F224G_RV

P-GCT TCG AAT TCT TAC AGG CCC AGG CCG TGG CGG CCC TCG G

V1a_del_FW

AAG GAT CCG ATG GCC TCC TCC GAG GAC GTC

H222S_REV

TTC GAA TTC TTA GGC GCC GGT GGA GTG GCG GCC