Supporting information for Campbell et al. (2002) Proc. Natl. Acad. Sci. USA 99 (12), 7877–7882. (10.1073/pnas.082243699)

Table 4. Summary of photobleach data

 

Extinction coefficient

e (M–1∙cm–1)*

e Q (M–1∙cm–1)

Quantum yield of photobleach, Q

Percent contribution of each component

Fast

Slow

Fast

Slow

Fast

Slow

DsRed

57,000

0.11

0.010

1.9 ´ 10–6

1.8 ´ 10–7

54 %

46 %

75,000

1.4 ´ 10–6

1.3 ´ 10–7

T1

35,000

0.069

0.0057

2.0 ´ 10–6

1.6 ´ 10–7

47 %

53 %

dimer2

60,000

0.16

0.0084

2.7 ´ 10–6

1.4 ´ 10–7

65 %

35 %

tdimer2(12)

120,000

(2 chromophores)

0.14

0.0066

2.4 ´ 10–6

1.1 ´ 10–7

65 %

35 %

mRFP1

44,000

3.3

0.076

7.6 ´ 10–5

1.7 ´ 10–6

60 %

40 %

EGFP

56,000

0.46

8.3 ´ 10–6

100 %

*The two values for the extinction coefficient of DsRed are the value determined in this work (57,000 M–1∙cm–1) and our previous estimate (75,000 M–1∙cm–1) (1). The extinction coefficient of EGFP is taken from ref. 2.

References

1. Baird, G. S., Zacharias, D. A. & Tsien, R. Y. (2000) Proc. Natl. Acad. Sci. USA 97, 11984–11989.

2. Tsien, R. Y. (1998) Annu. Rev. Biochem. 67, 509–544.