Supporting information for Campbell et al. (2002) Proc. Natl. Acad. Sci. USA 99 (12), 7877–7882. (10.1073/pnas.082243699)
Fig. 8.
Fluorescent images of red fluorescent proteins expressed in E. coli. E. coli strain JM109(DE3) was transformed with either DsRed, T1, dimer2, or mRFP1, plated on LB/agar supplemented with ampicillin, and incubated 12 h at 37°C then 8 h at 20°C before the plate was imaged with a digital camera. (A) T1, dimer2, and mRFP1 all appear of similar brightness when excited at 540 nm and imaged with a 575 nm (long pass) emission filter. Almost no fluorescence is visible for identically treated E. coli transformed with DsRed. (B) When excited at 560 nm and imaged with a 610 (long pass) filter, mRFP1 appears brighter because of its 25-nm red shift. (C) The monomer mRFP1 does not contain a green fluorescent component and is thus very dim in comparison to T1 and dimer2 when excited at 470 nm, a wavelength suitable for excitation of EGFP. (D) A digital color photograph of the same plate taken after 5 days at room temperature reveals the orange and purple hues of T1 and mRFP1, respectively.