Table 1. Primer sequences used in these experiments
Supporting Information for Prevost et al. (2002) Proc. Natl. Acad. Sci. USA, 10.1073/pnas.142053899. |
Construct name | Primer sequence |
GST-EphB1 | 5’-CCTGAATTCGTGGCTGCGATGGAAGAAACG-3’ 5’-CCTGAATTCATCCCCGTTGCAGTAGAGTTTG-3’ |
GST-DEphB1 | 5’-CCTGGATCCGTGGCTGCGATGGAAGAAACG-3’ 5’-GAAGAATTCAAGCTTCTTCATCAGCCTTCCCCCAAAGTCC-3’ 5’-GTTAAGCTTACATCTCTGGTGATTGCTCGGG-3’ 5’-CCTGAATTCATCCCCGTTGCAGTAGAGTTTG-3’ |
GST-ephrinA4 | 5’-TTAGGATCCCTCTGGGCCGCGTTCCTCGGC-3’ 5’-GGCTCACAGAATTCGCAGAAGA-3’ |
His-6-EphA4 | 5’-TTTGCGGCCGCCACAGGTTCCAGGGTATAC-3’ 5’-TTTCTCGAGGACCAGAAGGACTGTGGAGTT-3’ |
His-6-DEphA4 | 5’-TTTGCGGCCGCCACCCAAGTGGACATTGGTGA-3’ 5’-TTTGAATTCTGATTCATAGTAGTACAGGTT-3’ 5’-TTTGAATTCGTCAAAATTGACACCATTGCT-3’ 5’-TTTCTCGAGGACCAGAAGGACTGTGGAGTT-3’ |
His-6-EphB1 | 5’-TTTAGATCTCTCCCAGGTGGACTTTGGG-3’ 5’-CCCCTCGAGATCGTCAGTCAGAGTCTGGAA-3’ |
His-6-DEphB1 | 5’-TTTAGATCTCTCCCAGGTGGACTTTGGG-3’ 5’-TTTGAATTCAGTCTCATAGTAATACAAGTT-3’ 5’-TTTGAATTCCTCAAAGTAGACACCATTGCT-3’ 5’-CCCCTCGAGATCGTCAGTCAGAGTCTGGAA-3’ |
His-6-ephrinA4 | 5’-TTTCCATGGCTCTGCGCGGGGGCTCCAGC-3’ 5’-TTTCTCGAGTAGCAAGAGACAGAGGGGGCT-3’ |
His-6-DephrinA4 | 5’-TTTCCATGGACTACGAAGGCCCAGGGCCAGGGCCC-3’ 5’-TTTCTCGAGTAGCAAGAGACAGAGGGGGCT-3’ |
His-6-ephrinB2 | 5’-TTTAGATCTGAGAAGGGACTCCGTGTGGAAG-3’ 5’-TTTCTCGAGTTCGGAACCGAGGATGTTGTT-3’ |
His-6-DephrinB2 | 5’-TTTAGATCTAGTGCACTCTAAAACTGTTGGC-3’ 5’-TTTCTCGAGTTCGGAACCGAGGATGTTGTT-3’ |
