The genome-wide expression response to telomerase deletion in Saccharomycescerevisiae

Supporting information for Nautiyal et al. (2002) Proc. Natl. Acad. Sci. USA, 10.1073/pnas.142162499.

Fig. 10. An environmental stress response (ESR) is evoked in Dtlc1 mutants. The expression profiles of ~900 genes from the previously defined environmental stress response (1) were clustered with data from the Dtlc1 time courses. The average peak of induction of ESR genes induced in the TDR was about 5-fold. Examples of genes from various clusters are provided. Conditions used for clustering analysis were: 37°C heat shock, variable temperature shocks, hydrogen peroxide, menadione, DTT, diamide, osmotic shock, amino acid starvation, nitrogen depletion, diauxic shift, stationary phase, various carbon sources and continuous temperatures (1, 2). Data for variable temperature shocks and growth at single temperatures are not shown. For clarity, gene expression changes of less than 1.5-fold are masked in black. About 50% of the genes that are upregulated in the ESR were also upregulated in the TDR. Notably, three genes that are normally repressed in the ESR, RRP43, EMG1, and RPL37A are all induced at least three fold in the TDR. These genes are also part of the "TDR signature" set of genes. Rrp43p is a component of the exosome, a 3' to 5' exoribonuclease complex that is involved in processing of rRNA, snoRNAs and U4 snRNA (3-6). Emg1p is involved in 40s ribosome biogenesis and 18s rRNA maturation (7). Rpl37ap is a ribosomal protein (8, 9). The deviation of the gene expression pattern from that normally seen in the ESR suggests that these genes may have alternate activities which are enacted within the context of a Dtlc1 mutant.

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