Supporting information for Smith et al. (2002) Proc. Natl. Acad. Sci. USA, 10.1073/pnas.122612899.
Table 4. WAVE gradients for mutation detection
| p53 exon 2/3 62°C | p53 exon 4 62°C | p53 exon 4 63°C |
Time, min | %A | %B | Time, min | %A | %B | Time, min | %A | %B |
0 | 43 | 57 | 0 | 44 | 56 | 0 | 45 | 55 |
0.1 | 38 | 62 | 0.1 | 39 | 61 | 0.1 | 40 | 60 |
3.6 | 31 | 69 | 3.6 | 32 | 68 | 3.6 | 33 | 67 |
3.7 | 0 | 100 | 3.7 | 0 | 100 | 3.7 | 0 | 100 |
4.2 | 0 | 100 | 4.2 | 0 | 100 | 4.2 | 0 | 100 |
4.3 | 43 | 57 | 4.3 | 44 | 56 | 4.3 | 45 | 55 |
6.3 | 43 | 57 | 6.3 | 44 | 56 | 6.3 | 45 | 55 |
| |
| p53 exon 5 62°C | p53 exon 5 64°C | p53 exon 5 66°C |
Time, min | %A | %B | Time, min | %A | %B | Time, min | %A | %B |
0 | 46 | 54 | 0 | 49 | 51 | 0 | 51 | 49 |
0.1 | 41 | 59 | 0.1 | 44 | 56 | 0.1 | 46 | 54 |
3.6 | 34 | 66 | 3.6 | 37 | 63 | 3.6 | 39 | 61 |
3.7 | 0 | 100 | 3.7 | 0 | 100 | 3.7 | 0 | 100 |
4.2 | 0 | 100 | 4.2 | 0 | 100 | 4.2 | 0 | 100 |
4.3 | 46 | 54 | 4.3 | 49 | 51 | 4.3 | 51 | 49 |
6.3 | 46 | 54 | 6.3 | 49 | 51 | 6.3 | 51 | 49 |
| |
| p53 exon 6 60°C | p53 exon 6 62°C | p53 exon 7 61°C |
Time, min | %A | %B | Time, min | %A | %B | Time, min | %A | %B |
0 | 46 | 54 | 0 | 47 | 53 | 0 | 47 | 53 |
0.1 | 41 | 59 | 0.1 | 42 | 58 | 0.1 | 42 | 58 |
3.6 | 34 | 66 | 3.6 | 35 | 65 | 3.6 | 35 | 65 |
3.7 | 0 | 100 | 3.7 | 0 | 100 | 3.7 | 0 | 100 |
4.2 | 0 | 100 | 4.2 | 0 | 100 | 4.2 | 0 | 100 |
4.3 | 46 | 54 | 4.3 | 47 | 53 | 4.3 | 47 | 53 |
6.3 | 46 | 54 | 6.3 | 47 | 53 | 6.3 | 47 | 53 |
| |
| p53 exon 7 65°C | p53 exon 8/9 60°C | p53 exon 8/9 62°C |
Time, min | %A | %B | Time, min | %A | %B | Time, min | %A | %B |
0 | 51 | 49 | 0 | 44 | 56 | 0 | 50 | 50 |
0.1 | 46 | 54 | 0.1 | 39 | 61 | 0.1 | 45 | 55 |
3.6 | 39 | 61 | 3.6 | 32 | 68 | 3.6 | 38 | 62 |
3.7 | 0 | 100 | 3.7 | 0 | 100 | 3.7 | 0 | 100 |
4.2 | 0 | 100 | 4.2 | 0 | 100 | 4.2 | 0 | 100 |
4.3 | 51 | 49 | 4.3 | 44 | 56 | 4.3 | 50 | 50 |
6.3 | 51 | 49 | 6.3 | 44 | 56 | 6.3 | 50 | 50 |
| |
| p53 exon 10 57°C | p53 exon 10 62°C | p53 exon 10 64°C |
Time, min | %A | %B | Time, min | %A | %B | Time, min | %A | %B |
0 | 45 | 55 | 0 | 49 | 51 | 0 | 52 | 48 |
0.1 | 40 | 60 | 0.1 | 44 | 56 | 0.1 | 47 | 53 |
3.6 | 33 | 67 | 3.6 | 37 | 63 | 3.6 | 40 | 60 |
3.7 | 0 | 100 | 3.7 | 0 | 100 | 3.7 | 0 | 100 |
4.2 | 0 | 100 | 4.2 | 0 | 100 | 4.2 | 0 | 100 |
4.3 | 45 | 55 | 4.3 | 49 | 51 | 4.3 | 52 | 48 |
6.3 | 45 | 55 | 6.3 | 49 | 51 | 6.3 | 52 | 48 |
| |
| p53 exon 11 61°C | APC 1A 57C | APC 1A 58C |
Time, min | %A | %B | Time, min | %A | %B | Time, min | %A | %B |
0 | 45 | 55 | 0 | 45 | 55 | 0 | 47 | 53 |
0.1 | 40 | 60 | 0.1 | 40 | 60 | 0.1 | 42 | 58 |
3.6 | 33 | 67 | 3.6 | 33 | 67 | 3.6 | 35 | 65 |
3.7 | 0 | 100 | 3.7 | 0 | 100 | 3.7 | 0 | 100 |
4.2 | 0 | 100 | 4.2 | 0 | 100 | 4.2 | 0 | 100 |
4.3 | 45 | 55 | 4.3 | 45 | 55 | 4.3 | 47 | 53 |
6.3 | 45 | 55 | 6.3 | 45 | 55 | 6.3 | 47 | 53 |
| |
| APC 1B 56C | APC 1B 58C | |
Time, min | %A | %B | Time, min | %A | %B | | | |
0 | 45 | 55 | 0 | 49 | 51 | | | |
0.1 | 40 | 60 | 0.1 | 44 | 56 | | | |
3.6 | 33 | 67 | 3.6 | 37 | 63 | | | |
3.7 | 0 | 100 | 3.7 | 0 | 100 | | | |
4.2 | 0 | 100 | 4.2 | 0 | 100 | | | |
4.3 | 45 | 55 | 4.3 | 49 | 51 | | | |
6.3 | 45 | 55 | 6.3 | 49 | 51 | | | |
