Budovskaya et al. 10.1073/pnas.0501046102. |
Fig. 5. The full-length Atg1 was phosphorylated by cAMP-dependent protein kinase (PKA) in vitro at the serine residues within the two consensus PKA sites. The residues at positions 501 and 515 in the two PKA sites are indicated: S, serine; A, alanine. Note that Atg1 is a protein kinase that catalyzes an autophosphorylation reaction. This autophosphorylation retards the mobility of this protein in SDS/PAGE gels and causes the wild-type Atg1 to run as a doublet. The slower migrating species is the autophosphorylated form of the protein. Note that PKA phosphorylation did not affect the mobility of Atg1.
Fig. 6. Alteration of the serine residues within the PKA sites in Atg1 did not influence Atg1 protein kinase activity. (A) The indicated versions of Atg1 were precipitated, and in vitro phosphorylation reactions were performed as described in Methods. (Top) Autophosphorylation assay assessing label incorporation into Atg1. KD, kinase-dead. (Middle) An Atg1 in vitro phosphorylation assay using the myelin basic protein as substrate. (Bottom) A Western immunoblot control showing the relative levels of Atg1 present in the kinase reactions above. (B) Alteration of the consensus PKA sites did not influence the autophosphorylation of Atg1 in vivo. Shown is a Western immunoblot assessing the relative levels of the autophosphorylated and nonautophosphorylated forms of the indicated Atg1 variants.
Table 2. A list showing the proteins that contain the 553 consensus PKA sites present in the Saccharomyces cerevisiae proteome
Sequence name | Site | S. cer. | S. bay. | S. kud. | S. mik. | S. cas. | S. klu. | C. alb. |
YAL056W/GPB2 | 454 | RRASV | RRNSV | RRNSV | RRNSV | ----- | ----- | ----- |
YAL048C/GON1 | 432 | RRRSG | RRRSG | RRRSG | RRRSG | ----- | ----- | ----- |
YAL038W/CDC19 | 19 | RRTSI | RRTSI | ----- | RRTSI | RRTSI | RRTSI | RRSSI |
YAL029C/MYO4 | 1096 | RRESG | RRESG | ----- | RRESG | ----- | ----- | ----- |
YAL024C/LTE1 | 1122 | RRQTI | RRQTI | RRQTI | RRQTI | RRQTI | ----- | ----- |
YAL017W/FUN31 | 182 | RRSSL | RRRSM | RRRSL | RRRSL | ----- | ----- | ----- |
YAR002W/NUP60 | 7 | RRASA | ----- | ----- | ----- | ----- | ----- | ----- |
YBL113C | 568 | RRETI | ----- | ----- | ----- | ----- | ----- | ----- |
YBL101C/ECM21 | 133 | RRTSG | ----- | ----- | ----- | ----- | ----- | ----- |
YBL101C/ECM21 | 772 | RRPSV | RRPSM | RRPSV | RRPSV | ----- | RRPSS | ----- |
YBL072C/RPS8A | 194 | RRLTA | RRLTA | RRLTA | RRLTA | RRLTA | RRLTA | RRLTA |
YBL060W | 414 | RRTSL | ----- | ----- | ----- | ----- | ----- | ----- |
YBL056W/PTC3 | 321 | RRKSI | ----- | RRKSI | RRKSI | ----- | ----- | ----- |
YBL054W | 277 | RRNSF | RRNSF | RRNSF | RRNSF | RRSSF | RRGSM | ----- |
YBL054W | 305 | RRSSF | RRSSF | RRSSF | RRSSF | ----- | RRSSF | ----- |
YBL054W | 315 | RRSSF | RRSSF | RRSSV | RRSSF | RRSSF | RRSSM | ----- |
YBL054W | 330 | RRASL | RRPSL | RRASL | RRASM | RRGSM | RRESF | ----- |
YBL042C/FUI1 | 457 | RRGSY | RRGSY | RRGSY | RRGSY | ----- | ----- | ----- |
YBL037W/APL3 | 724 | RRNTI | RRNTV | RRSTT | RRNTM | ----- | ----- | ----- |
YBL029W | 350 | RRGTY | RRGTY | RRGTY | RRGTY | RRATY | RRATY | RRGSY |
YBL023C/MCM2 | 794 | RRESI | RRESI | ----- | RRESI | RRESI | RRESI | ----- |
YBL014C/RRN6 | 434 | RRISW | RRISW | RRISW | ----- | ----- | ----- | ----- |
YBL011W/SCT1 | 110 | RRVSF | RRVSF | RRVSF | RRVSF | RRVSF | ----- | RRISF |
YBL008W/HIR1 | 236 | RRPSW | RRPSW | RRPSW | RRPSW | RRLSW | RRLSW | RRMSW |
YBL006C/LDB7 | 128 | RRRTI | ----- | ----- | ----- | ----- | ----- | ----- |
YBL004W/UTP20 | 995 | RRMTG | RRMTG | RRMTG | RRMTG | ----- | RRMTG | ----- |
YBR007C | 499 | RRRTL | RRRTL | ----- | ----- | ----- | ----- | ----- |
YBR028C | 70 | RRSSA | RRSSA | RRSSA | RRSSI | RRSSI | RRSSL | RRKSS |
YBR028C | 87 | RRFSI | RRFSI | RRFSI | RRFSI | RRASG | ----- | ----- |
YBR033W/EDS1 | 171 | RRSSL | RRSSL | ----- | RRGSS | RRNSD | ----- | ----- |
YBR043C/AQR2 | 172 | RRTTY | RRTTY | RRTTY | RRTTY | ----- | ----- | ----- |
YBR071W | 204 | RRTSL | RRTSL | RRTSL | RRTSL | ----- | ----- | ----- |
YBR089C-A/NHP6B | 29 | RRLSA | ----- | ----- | ----- | ----- | ----- | ----- |
YBR102C/EXO84 | 73 | RRLSI | RRLSI | RRLSI | RRLSI | RRLSI | RRLSV | ----- |
YBR140C/IRA1 | 1750 | RRNSC | RRNSC | ----- | RRNSC | RRNTC | ----- | RRNST |
YBR147W | 38 | RRQSA | RRQSA | RRQSA | RRQSA | ----- | ----- | ----- |
YBR159W | 264 | RRSSL | RRSSL | RRSSL | RRSSL | RRTSM | ----- | ----- |
YBR182C/SMP1 | 104 | RRATV | RRRTA | RRATV | ----- | ----- | ----- | ----- |
YBR184W | 136 | RRQSA | ----- | ----- | ----- | ----- | ----- | ----- |
YBR198C/TAF90 | 758 | RRRTV | RRRTV | RRRTV | RRRTV | RRRTI | RRRTI | ----- |
YBR220C | 85 | RRRSW | RRRSW | RRRSW | RRRSW | RRRSW | RRRSW | RRRSW |
YBR229C/ROT2 | 816 | RRSSM | ----- | RRSSK | RRSSK | RRSSK | ----- | RRSSK |
YBR233W/PBP2 | 376 | RRLTI | RRLSI | ----- | RRLSI | ----- | ----- | ----- |
YBR270C | 134 | RRLSI | RRLSL | RRLSI | RRLSM | ----- | ----- | ----- |
YBR274W/CHK1 | 283 | RRASF | ----- | ----- | RRVSF | ----- | ----- | ----- |
YBR280C | 268 | RRMSG | RRMSG | ----- | ----- | ----- | ----- | ----- |
YCL051W/LRE1 | 368 | RRRSG | RRRSG | RRRSG | RRRSG | ----- | ----- | ----- |
YCL029C/BIK1 | 87 | RRTTM | RRTTM | RRTTM | RRATM | ----- | RRATL | ----- |
YCL010C/SGF29 | 136 | RRGSA | ----- | ----- | ----- | ----- | ----- | ----- |
YCR006C | 128 | RRLSG | ----- | ----- | ----- | ----- | ----- | ----- |
YCR011C/ADP1 | 536 | RRVSI | RRVSI | RRVSI | RRVSI | RRVSI | RRVSI | RRVSI |
YCR023C | 310 | RRQSV | RRQSV | RRQSV | RRQSV | RRQSV | ----- | ----- |
YCR030C/SYP1 | 396 | RRPTY | ----- | RRPTY | RRPTY | RRGTF | ----- | ----- |
YCR032W/BPH1 | 1331 | RRESG | RRESG | RRESG | ----- | ----- | ----- | ----- |
YCR038W-A | 5 | RRTSV | ----- | ----- | ----- | ----- | ----- | ----- |
YCR077C/PAT1 | 454 | RRSSY | RRSSY | RRSSY | RRSSY | RRSSY | RRSSY | ----- |
YDL240W/LRG1 | 546 | RRESV | RRESV | ----- | RRESV | ----- | RRQSV | ----- |
YDL236W/PHO13 | 222 | RRPSY | RRPSY | RRPSY | RRPSY | RRPSY | ----- | ----- |
YDL233W | 414 | RRNSG | RRNSG | RRNSG | RRNSG | ----- | ----- | ----- |
YDL231C/BRE4 | 745 | RRYSG | RRYSG | RRYSG | RRYSG | RRYSG | RRNSG | ----- |
YDL231C/BRE4 | 988 | RRDSV | RRDSV | RRDSV | RRVSF | RRDSV | RRDSV | ----- |
YDL228C | 116 | RRLTG | ----- | ----- | ----- | ----- | ----- | ----- |
YDL227C/HO | 69 | RRRTV | RRRTI | RRRTV | RRRTV | ----- | ----- | ----- |
YDL224C/WHI4 | 560 | RRLSF | RRLSF | RRLSF | RRLSF | RRLSF | RRLSF | RRLSF |
YDL214C/PRR2 | 640 | RRITI | RRITV | ----- | ----- | ----- | ----- | ----- |
YDL199C | 66 | RRGSL | RRGSL | RRGSL | RRGSL | ----- | ----- | ----- |
YDL189W/RBS1 | 432 | RRASV | ----- | ----- | ----- | ----- | ----- | ----- |
YDL176W | 402 | RRESF | RRESF | RRESF | RRESF | ----- | ----- | ----- |
YDL176W | 436 | RRNTF | RRNTF | RRNTF | RRNTF | ----- | RRNTC | ----- |
YDL171C/GLT1 | 2067 | RRGTI | RRGTI | RRGTI | RRGTI | ----- | RRGTI | ----- |
YDL170W/UGA3 | 38 | RRLSF | RRLSF | ----- | ----- | ----- | ----- | ----- |
YDL161W/ENT1 | 157 | RRGTG | RRRTN | RRGTG | RRGTG | RRGTR | ----- | ----- |
YDL153C/SAS10 | 412 | RRRTL | RRRTL | RRRTL | RRKTL | RRRTL | ----- | ----- |
YDL102W/CDC2 | 839 | RRDSC | RRDSC | ----- | RRDSC | RRDSC | RRDSC | ----- |
YDL060W/TSR1 | 621 | RRYTI | RRYTI | RRYTI | RRYTI | RRYTI | ----- | ----- |
YDL051W/LHP1 | 16 | RRNSF | RRNSF | RRNSF | RRNSF | RRNSF | RRGSY | ----- |
YDL025C | 74 | RRSTI | ----- |
| ----- | ----- | ----- | ----- |
YDL016C | 52 | RRTSL | ----- | ----- | ----- | ----- | ----- | ----- |
YDR005C/MAF1 | 98 | RRISF | RRISF | RRISF | RRISF | RRMSV | RRISV | RRASS |
YDR014W/RAD61 | 125 | RRRTY | ----- | RRRTY | RRRTY | ----- | ----- | ----- |
YDR017C/KCS1 | 534 | RRDSG | RRDSG | RRDSG | RRDSG | RRDSG | ----- | ----- |
YDR034W-B | 24 | RRESG | ----- | ----- | ----- | ----- | ----- | ----- |
YDR082W/STN1 | 58 | RRLSM | ----- | ----- | ----- | ----- | ----- | ----- |
YDR085C/AFR1 | 523 | RRNSL | ----- | ----- | ----- | ----- | ----- | ----- |
YDR094W | 83 | RRCSA | ----- | ----- | ----- | ----- | ----- | ----- |
YDR099W/BMH2 | 57 | RRASW | RRASW | RRASW | RRASW | RRASW | RRASW | RRASW |
YDR103W/STE5 | 895 | RRSSF | RRGSL | RRGSI | RRSSI | ----- | ----- | ----- |
YDR104C/SPO71 | 356 | RRKSA | ----- | ----- | ----- | ----- | ----- | ----- |
YDR122W/KIN1 | 385 | RRATL | ----- | RRATL | RRATL | RRATL | ----- | ----- |
YDR122W/KIN1 | 796 | RRESL | ----- | RRESL | RRESL | RRESL | RRESL | ----- |
YDR137W/RGP1 | 447 | RRKSY | RRKSE | RRRSE | RRKSE | ----- | RRKSR | ----- |
YDR169C/STB3 | 283 | RRSSI | RRSSI | RRSSI | RRSSI | RRMSR | RRSSS | RRKSS |
YDR172W/SUP35 | 338 | RRYTI | RRYTI | RRYTI | ----- | RRYTL | ----- | RRYTI |
YDR190C/RVB1 | 386 | RRATV | RRATV | ----- | ----- | ----- | ----- | ----- |
YDR207C/UME6 | 642 | RRLSA | RRLSA | ----- | RRLSA | RRLSA | RRLSA | ----- |
YDR216W/ADR1 | 227 | RRASF | RRASF | ----- | RRASF | RRASF | RRGSF | ----- |
YDR218C/SPR28 | 369 | RRNSA | RRSTP | RRSSS | RRNSS | ----- | ----- | ----- |
YDR241W/BUD26 | 11 | RRETV | ----- | ----- | ----- | ----- | ----- | ----- |
YDR251W/PAM1 | 468 | RRQSF | RRQSF | RRQSF | RRQSF | ----- | ----- | ----- |
YDR277C/MTH1 | 109 | RRSSV | RRSST | RRSSV | ----- | ----- | ----- | ----- |
YDR283C/GCN2 | 574 | RRRSF | RRRSF | ----- | RRRSF | ----- | ----- | RRLSN |
YDR285W/ZIP1 | 266 | RRISG | RRISG | RRISG | RRISG | ----- | ----- | ----- |
YDR293C/SSD1 | 161 | RRHSL | RRHSL | RRHSL | RRHSL | RRHSL | RRHSL | RRHSL |
YDR293C/SSD1 | 500 | RRSSL | RRSSL | RRSSL | RRSSL | RRNSL | RRGSL | RRGSL |
YDR297W/SUR2 | 310 | RRVTI | RRVTI | RRVTI | RRVTI | ----- | RRVTI | ----- |
YDR301W/CFT1 | 1323 | RRKSI | RRNTA | ----- | ----- | ----- | ----- | ----- |
YDR309C/GIC2 | 342 | RRNSI | RRNSI | RRNSV | RRNSI | RRSSQ | RRKSD | ----- |
YDR338C | 28 | RRISL | RRISL | ----- | RRISL | RRISL | RRISL | ----- |
YDR338C | 187 | RRPSF | RRPSF | RRPSF | RRPSF | RRHSF | ----- | ----- |
YDR352W | 141 | RRRSA | RRRSA | RRRSA | RRRSA | ----- | ----- | ----- |
YDR356W/NUF1 | 897 | RRLSF | ----- | ----- | ----- | ----- | ----- | ----- |
YDR360W | 118 | RRLTL | ----- | ----- | ----- | ----- | ----- | ----- |
YDR362C/TFC6 | 124 | RRNTV | ----- | ----- | ----- | ----- | ----- | ----- |
YDR385W/EFT2 | 710 | RRATY | RRATY | RRATY | RRATY | RRATY | RRATY | RRATY |
YDR387C | 109 | RRITL | RRITL | RRITL | RRITL | RRKTL | ----- | ----- |
YDR389W/SAC7 | 507 | RRTSL | RRTSL | RRTSL | RRTSL | ----- | ----- | ----- |
YDR421W/ARO80 | 18 | RRRTY | RRVTR | RRRTY | RRRTY | ----- | ----- | ----- |
YDR422C/SIP1 | 430 | RRSSF | RRSSF | RRSSF | RRSSF | ----- | ----- | ----- |
YDR436W/PPZ2 | 115 | RRSSM | RRSST | RRSSM | RRSSM | ----- | ----- | ----- |
YDR438W | 69 | RRKSL | ----- | ----- | ----- | ----- | ----- | ----- |
YDR443C/SSN2 | 605 | RRKSV | RRRSV | RRKSV | RRKSV | RRKSV | RRRSV | ----- |
YDR443C/SSN2 | 1233 | RRLSG | RRLSG | RRLSG | RRLSG | RRLSG | RRLSS | ----- |
YDR457W/TOM1 | 1683 | RRNTY | RRNTY | RRNTY | RRNTY | ----- | ----- | ----- |
YDR459C | 10 | RRRSW | ----- | ----- | ----- | ----- | ----- | ----- |
YDR463W/STP1 | 269 | RRDTY | RRDTY | RRDTY | RRDTY | RRDTY | RRDTY | RRDTY |
YDR474C | 268 | RRKSM | ----- | ----- | ----- | ----- | ----- | ----- |
YDR499W/LCD1 | 2 | RRETV | ----- | ----- | ----- | ----- | ----- | ----- |
YDR505C/PSP1 | 235 | RRSSY | RRSSY | RRSSY | RRSSY | RRSSY | ----- | RRPSY |
YDR525W | 60 | RRGSG | ----- | ----- | ----- | ----- | ----- | ----- |
YDR528W/HLR1 | 61 | RRVSG | ----- | ----- | RRASG | ----- | ----- | ----- |
YDR545W/YRF1-1 | 1572 | RRETI | ----- | ----- | ----- | ----- | ----- | ----- |
YEL077C | 1053 | RRETI | ----- | ----- | ----- | ----- | ----- | ----- |
YEL063C/CAN1 | 35 | RRGSI | RRGSL | RRGSL | RRGSL | ----- | ----- | ----- |
YEL043W | 151 | RRNTI | RRNTV | RRNTI | ----- | RRNTL | ----- | ----- |
YEL032W/MCM3 | 788 | RRSTA | ----- | ----- | ----- | ----- | RRASL | ----- |
YEL023C | 89 | RRLTI | RRLTF | RRLSI | RRLSI | ----- | ----- | ----- |
YER005W/YND1 | 525 | RRFSG | ----- | RRFSG | ----- | ----- | ----- | ----- |
YER007W/PAC2 | 77 | RRITF | ----- | ----- | ----- | ----- | ----- | ----- |
YER045C/ACA1 | 102 | RRISI | RRISI | RRISI | RRISI | RRISI | ----- | RRISI |
YER049W | 166 | RRISF | RRISF | RRISF | RRISF | RRISF | RRVSF | RRVSF |
YER051W | 415 | RRFTF | RRFTF | ----- | ----- | ----- | ----- | ----- |
YER065C/ICL1 | 50 | RRGTF | RRGTF | RRGTF | RRGTF | RRGTF | RRGTF | ----- |
YER072W/VTC1 | 89 | RRGSG | RRGSG | RRGSG | RRGSG | ----- | ----- | ----- |
YER088C/DOT6 | 279 | RRSSF | RRSSF | RRSSL | RRSSF | RRSSF | RRGSM | ----- |
YER088C/DOT6 | 310 | RRGSL | ----- | ----- | RRGSL | ----- | RRSSF | ----- |
YER088C/DOT6 | 319 | RRSSF | RRSSF | RRSSF | RRSSF | ----- | RRSSM | ----- |
YER088C/DOT6 | 332 | RRSSM | RRSSI | RRSSV | RRSSV | ----- | RRESF | ----- |
YER088C/DOT6 | 365 | RRESV | RRESV | RRESV | RRESV | RRESI | RRGSI | ----- |
YER093C | 16 | RRRSV | ----- | ----- | ----- | ----- | ----- | ----- |
YER099C/PRS2 | 87 | RRITA | ----- | RRITA | RRITA | RRITA | ----- | RRITA |
YER102W/RPS8B | 194 | RRLTA | RRLTA | RRLTA | RRLTA | RRLTA | RRLTA | RRLTA |
YER119C-A | 116 | RRVSF | ----- | ----- | ----- | ----- | ----- | ----- |
YER125W/RSP5 | 758 | RRFTI | RRFTI | RRFTI | RRFTI | RRFTI | ----- | RRFTI |
YER129W/PAK1 | 1071 | RRRSI | RRRSI | RRRSI | RRRSI | RRGSI | RRGSL | ----- |
YER130C | 85 | RRLSI | RRLSI | RRLSI | RRLSI | ----- | RRLSI | ----- |
YER132C/PMD1 | 1079 | RRLSY | RRLSY | RRLSY | RRLSY | ----- | ----- | ----- |
YER140W | 19 | RRNSA | RRNSA | RRNSA | RRNSA | RRSSV | ----- | ----- |
YER158C | 25 | RRDSL | ----- | RRDSL | ----- | ----- | ----- | ----- |
YER158C | 143 | RRHSL | ----- | RRHSL | RRHSL | ----- | ----- | ----- |
YER164W/CHD1 | 750 | RRISI | RRISI | RRISI | RRISI | RRISI | RRISI | ----- |
YER177W/BMH1 | 57 | RRASW | RRASW | RRASW | RRASW | RRASW | RRASW | RRASW |
YER182W/FMP10 | 130 | RRETV | RRETV | RRETV | ----- | ----- | ----- | ----- |
YER184C | 605 | RRNSI | ----- | ----- | ----- | ----- | ----- | ----- |
YER190W/YRF1-2 | 1458 | RRETI | ----- | ----- | ----- | ----- | ----- | ----- |
YFL050C/ALR2 | 611 | RRKTM | RRKTM | RRKTM | RRKTM | RRKTM | RRKTM | ----- |
YFL045C/SEC53 | 122 | RRGTF | RRGTF | ----- | RRGTF | RRGTF | RRGTF | RRGTF |
YFL033C/RIM15 | 1091 | RRKSL | RRKSL | RRKSL | RRKSL | RRRSP | RRHST | ----- |
YFL033C/RIM15 | 1618 | RRRSG | RRRSG | RRRSG | RRRSG | RRESG | RRRSG | RRESS |
YFL028C/CAF16 | 198 | RRCSV | RRCSV | RRCSV | RRCSV | ----- | RRCSV | ----- |
YFR006W | 399 | RRASC | ----- | RRASC | RRASC | ----- | RRASV | ----- |
YFR017C | 93 | RRSSG | RRSSG | ----- | RRSSG | ----- | ----- | ----- |
YFR021W/AUT10 | 285 | RRGTY | RRGTY | RRGTY | RRGTY | RRGTY | RRGTY | RRGTY |
YFR034C/PHO4 | 240 | RRSSG | RRSSG | RRSSG | RRSSG | ----- | ----- | ----- |
YFR046C/CNN1 | 266 | RRSSI | ----- | ----- | ----- | ----- | ----- | ----- |
YFR048W/RMD8 | 76 | RRSSG | RRSSG | RRSSG | RRSSG | ----- | RRLSF | ----- |
YFR056C | 105 | RRLTI | ----- | ----- | ----- | ----- | ----- | ----- |
YGL262W | 33 | RRISY | ----- | ----- | ----- | ----- | ----- | ----- |
YGL244W/RTF1 | 216 | RRDTY | ----- | ----- | ----- | ----- | ----- | ----- |
YGL236C/MTO1 | 21 | RRLTI | ----- | ----- | ----- | ----- | ----- | ----- |
YGL211W | 164 | RRQSL | RRQSL | RRQSL | ----- | ----- | ----- | ----- |
YGL205W/POX1 | 3 | RRTTI | RRTTV | RRTTI | RRTTI | ----- | ----- | ----- |
YGL197W/MDS3 | 754 | RRSSL | RRSSL | RRSSL | RRSSL | RRSSF | ----- | RRQSF |
YGL195W/GCN1 | 1384 | RRESV | RRESV | RRESV | ----- | RRESV | RRESA | ----- |
YGL180W/APG1 | 505 | RRPSL | RRPSL | RRPSL | RRPSL | RRPSV | RRPSL | RRSSS |
YGL180W/APG1 | 512 | RRLSI | RRLSI | RRLSI | RRLSI | RRLSI | RRISL | RRISL |
YGL179C/TOS3 | 553 | RRMSL | ----- | RRMSL | RRMSL | ----- | ----- | ----- |
YGL158W/RCK1 | 52 | RRSSL | RRSSL | ----- | RRSSL | ----- | ----- | ----- |
YGL150C/INO80 | 62 | RRDSI | ----- | ----- | ----- | ----- | ----- | ----- |
YGL141W/HUL5 | 323 | RRLSW | RRLSW | ----- | RRLSW | ----- | ----- | ----- |
YGL131C/SNT2 | 465 | RRNSY | ----- | ----- | ----- | ----- | ----- | ----- |
YGL114W | 414 | RRNTY | RRNTN | RRNTN | RRNTN | ----- | ----- | ----- |
YGL098W/USE1 | 85 | RRYSI | ----- | RRYSI | RRYSI | RRYSV | RRYSI | ----- |
YGL093W/SPC105 | 74 | RRVSF | RRVSF | RRVSF | RRVSF | RRVSF | RRVSF | RRVSF |
YGL092W/NUP145 | 748 | RRFTA | RRFTA | RRFTA | RRLTS | ----- | ----- | ----- |
YGL090W/LIF1 | 268 | RRKTV | ----- | ----- | ----- | ----- | ----- | ----- |
YGL021W/ALK1 | 255 | RRSSI | RRSSI | RRSSI | RRSSV | ----- | ----- | ----- |
YGL021W/ALK1 | 300 | RRDSM | RRDST | RRGSM | RRDSM | ----- | ----- | ----- |
YGL014W/PUF4 | 202 | RRQTF | RRQTF | RRQTF | RRQTF | RRQTF | RRQTF | ----- |
YGL013C/PDR1 | 709 | RRPSI | ----- | ----- | RRPSL | ----- | ----- | ----- |
YGL003C/CDH1 | 210 | RRLSA | ----- | ----- | ----- | RRTSV | ----- | RRKTN |
YGR032W/GSC2 | 836 | RRISF | RRISF | RRISF | RRISF | RRISF | RRITF | RRISF |
YGR032W/GSC2 | 1375 | RRYTL | RRYTL | RRYTL | RRYTL | RRYTL | RRISL | RRYTL |
YGR047C/TFC4 | 622 | RRITA | RRITA | RRITA | RRITA | ----- | ----- | ----- |
YGR064W | 25 | RRRTF | ----- | ----- | ----- | ----- | ----- | ----- |
YGR079W | 350 | RRKSG | RRKSG | RRKSG | RRKSG | RRESG | RRKSG | ----- |
YGR083C/GCD2 | 118 | RRATI | RRATI | RRATI | RRATI | RRASI | RRASL | ----- |
YGR113W/DAM1 | 28 | RRSSM | RRSSI | RRSSM | RRSSM | RRSSL | RRSSL | RRHSH |
YGR113W/DAM1 | 254 | RRKSI | RRKSI | RRKSI | RRKSI | RRKSI | RRKSI | RRVSR |
YGR114C | 56 | RRFSF | RRFSF | ----- | RRFSF | ----- | ----- | ----- |
YGR121C/MEP1 | 45 | RRKSA | RRKSA | RRKSA | RRKSA | RRKSA | RRKSA | RRKSA |
YGR150C | 664 | RRLTF | RRLTV | ----- | RRLTN | ----- | ----- | ----- |
YGR151C | 95 | RRKTL | ----- | ----- | ----- | ----- | ----- | ----- |
YGR156W/PTI1 | 5 | RRRTG | RRRTG | RRRTG | ----- | RRRTA | RRNTR | ----- |
YGR199W/PMT6 | 643 | RRQTL | RRQTL | ----- | ----- | ----- | ----- | ----- |
YGR211W/ZPR1 | 345 | RRITL | RRITL | RRITL | RRITL | ----- | RRITL | ----- |
YGR217W/CCH1 | 143 | RRRSF | RRRSF | RRRSL | RRRSF | ----- | ----- | ----- |
YGR217W/CCH1 | 344 | RRYSL | ----- | RRYSL | ----- | ----- | ----- | ----- |
YGR235C | 139 | RRRTW | RRRTW | ----- | RRRTW | ----- | ----- | ----- |
YGR237C | 134 | RRTSV | ----- | RRASV | RRTSV | ----- | ----- | ----- |
YGR237C | 346 | RRYTW | RRYTW | RRYTW | RRYTW | RRFTW | RRHTW | ----- |
YGR249W/MGA1 | 206 | RRQSI | RRQSI | RRQSI | RRQSI | ----- | ----- | ----- |
YGR296W/YRF1-3 | 1636 | RRETI | ----- | ----- | ----- | ----- | ----- | ----- |
YHL050C | 473 | RRETI | ----- | ----- | ----- | ----- | ----- | ----- |
YHL024W/RIM4 | 213 | RRYTY | RRYTY | RRYTY | RRYTY | RRCSY | ----- | ----- |
YHL024W/RIM4 | 522 | RRKSM | RRKSM | RRKSM | RRKSM | RRNSF | RRGSF | ----- |
YHL024W/RIM4 | 604 | RRSSY | RRSSY | RRSSY | RRSSY | ----- | RRGSY | ----- |
YHL023C/RMD11 | 984 | RRPSM | ----- | ----- | ----- | ----- | ----- | ----- |
YHL011C/PRS3 | 86 | RRITA | RRITA | RRITA | RRITA | RRITA | ----- | RRITV |
YHL004W/MRP4 | 11 | RRLSL | ----- | RRFSS | ----- | ----- | ----- | ----- |
YHR006W/STP2 | 255 | RRDTY | RRDTY | RRDTY | RRDTY | RRDTY | RRDTY | RRDTY |
YHR013C/ARD1 | 6 | RRATI | RRATI | RRATI | RRATI | RRATI | RRATI | ----- |
YHR017W/YSC83 | 132 | RRFTV | ----- | RRFTV | RRFTV | ----- | ----- | ----- |
YHR022C | 119 | RRTSI | ----- | ----- | ----- | ----- | ----- | ----- |
YHR032W | 12 | RRSSI | RRSSI | RRSSI | RRSSI | ----- | ----- | RRASI |
YHR048W | 138 | RRITF | RRTTF | RRITF | RRITF | RRPTF | ----- | ----- |
YHR050W/SMF2 | 37 | RRTTL | RRTTA | RRTTL | ----- | ----- | ----- | ----- |
YHR082C/KSP1 | 824 | RRLSM | ----- | RRLSL | RRLSM | RRDSE | ----- | ----- |
YHR082C/KSP1 | 881 | RRSSA | ----- | RRSSV | RRSSI | ----- | RRSSM | ----- |
YHR084W/STE12 | 582 | RRRTV | RRRTT | RRRTV | RRRTV | ----- | ----- | ----- |
YHR099W/TRA1 | 3437 | RRRSI | ----- | ----- | RRRSI | ----- | ----- | ----- |
YHR102W/KIC1 | 175 | RRQTM | RRQTM | RRQTM | RRQTM | RRQTM | RRQTM | ----- |
YHR102W/KIC1 | 642 | RRLTV | RRLTV | RRLTV | RRLTV | RRLTV | ----- | ----- |
YHR114W/BZZ1 | 573 | RRSTL | ----- | RRSTL | RRSTL | RRSTL | ----- | ----- |
YHR117W/TOM71 | 200 | RRASA | RRASA | RRASA | RRASA | RRASA | RRASA | RRATS |
YHR144C/DCD1 | 133 | RRQSL | ----- | ----- | ----- | ----- | ----- | ----- |
YHR165C/PRP8 | 1604 | RRFTL | RRFTL | ----- | RRFTL | ----- | RRFTL | RRFTL |
YHR181W/SVP26 | 133 | RRASF | RRASF | RRASF | RRASF | ----- | RRASF | ----- |
YHR219W | 400 | RRETI | ----- | ----- | ----- | ----- | ----- | ----- |
YIL177C | 1535 | RRETI | ----- | ----- | ----- | ----- | ----- | ----- |
YIL151C | 97 | RRPSI | RRPSI | RRPSI | RRPSI | RRPSL | ----- | ----- |
YIL125W/KGD1 | 907 | RRESL | ----- | RRETL | RRETL | ----- | ----- | ----- |
YIL107C/PFK26 | 641 | RRYSV | RRYSV | RRYSV | RRYSV | RRYSV | RRYSI | ----- |
YIL074C/SER33 | 40 | RRVSI | RRVSI | RRVSI | RRVSI | RRSSG | ----- | ----- |
YIL059C | 108 | RRKSV | ----- | ----- | ----- | ----- | ----- | ----- |
YIL047C/SYG1 | 857 | RRSSV | RRSSV | RRSSV | RRSSV | RRTTF | ----- | RRKST |
YIL046W/MET30 | 336 | RRLSG | RRLSG | RRLSG | RRLSG | RRLSG | RRLTG | ----- |
YIL043C/CBR1 | 298 | RRSTV | ----- | RRSTV | RRSTV | ----- | ----- | ----- |
YIL036W/CST6 | 263 | RRISI | RRISI | RRISI | RRISI | RRISI | RRISI | RRISI |
YIL033C/BCY1 | 142 | RRTSV | RRTSV | RRTSV | RRTSV | RRTSV | RRTSV | RRTSV |
YIL015W/BAR1 | 177 | RRESV | RRESV | RRESV | RRESV | RRESV | ----- | ----- |
YIL013C/PDR11 | 975 | RRLTF | ----- | ----- | ----- | ----- | ----- | ----- |
YIL005W/EPS1 | 138 | RRESM | RRESM | RRESM | ----- | ----- | ----- | ----- |
YIR010W/DSN1 | 247 | RRLSM | ----- | RRLSM | RRLSM | RRLSI | RRLSM | ----- |
YIR016W | 174 | RRNSM | RRNST | RRNSS | ----- | ----- | ----- | ----- |
YIR028W/DAL4 | 442 | RRGSL | RRGSL | RRGSL | RRGSL | ----- | ----- | ----- |
YIR033W/MGA2 | 252 | RRKSG | RRKSG | RRKSG | RRKSG | RRKSG | ----- | ----- |
YJL225C | 1535 | RRETI | ----- | ----- | ----- | ----- | ----- | ----- |
YJL206C | 326 | RRSSI | ----- | ----- | ----- | ----- | ----- | ----- |
YJL193W | 366 | RRITW | RRITW | ----- | RRITW | ----- | ----- | ----- |
YJL192C/SOP4 | 21 | RRGTI | ----- | ----- | ----- | ----- | ----- | ----- |
YJL187C/SWE1 | 376 | RRKSI | RRKSI | RRKSI | RRKSI | RRKSI | ----- | ----- |
YJL187C/SWE1 | 780 | RRPTA | RRPTA | RRPTA | RRPTA | RRPSA | ----- | ----- |
YJL182C | 18 | RRKSF | ----- | ----- | ----- | ----- | ----- | ----- |
YJL177W/RPL17B | 126 | RRRTY | ----- | ----- | ----- | ----- | ----- | ----- |
YJL165C/HAL5 | 214 | RRNSL | RRNSL | RRNSL | RRNSL | ----- | ----- | ----- |
YJL146W/IDS2 | 145 | RRSSI | RRSSI | RRSSI | RRSSI | RRSSI | ----- | ----- |
YJL141C/YAK1 | 203 | RRQSL | RRQSL | RRQSL | RRQSL | RRQSM | ----- | ----- |
YJL141C/YAK1 | 237 | RRLSA | ----- | RRLSA | RRLSA | RRLSA | RRLSA | ----- |
YJL141C/YAK1 | 292 | RRASL | RRASL | RRASL | RRASL | RRASL | RRASL | RRCSI |
YJL130C/URA2 | 1854 | RRFSI | RRFSF | ----- | RRASV | ----- | ----- | ----- |
YJL128C/PBS2 | 610 | RRPTY | RRPTY | RRPTY | RRPTY | ----- | ----- | ----- |
YJL126W/NIT2 | 249 | RRESW | ----- | ----- | ----- | ----- | ----- | ----- |
YJL104W/MIA1 | 42 | RRGTG | RRGTG | RRGTG | RRGTG | ----- | ----- | ----- |
YJL090C/DPB11 | 109 | RRFSF | ----- | ----- | ----- | ----- | ----- | ----- |
YJL084C | 835 | RRESV | ----- | RRESV | ----- | RRKSI | RRNSA | ----- |
YJL073W/JEM1 | 612 | RRNTF | RRNTF | ----- | RRNTF | ----- | ----- | ----- |
YJL070C | 6 | RRPSL | RRPSL | RRPSL | RRPSL | RRPSL | RRPSI | ----- |
YJL057C/IKS1 | 93 | RRASA | RRASG | RRASS | RRASS | ----- | ----- | RRSSS |
YJL051W | 714 | RRDSL | RRDSI | RRDSV | RRDSL | ----- | ----- | ----- |
YJL046W | 229 | RRFSG | RRFSG | RRFSG | RRFSG | RRLSG | RRRSG | RRRSG |
YJL042W/MHP1 | 78 | RRHSV | RRHSV | RRHSV | RRHSV | ----- | ----- | ----- |
YJL042W/MHP1 | 306 | RRASF | ----- | ----- | ----- | ----- | ----- | ----- |
YJL036W/SNX4 | 132 | RRVSL | RRVSL | RRVSL | RRVSL | RRVSL | ----- | ----- |
YJL020C/BBC1 | 644 | RRKSI | ----- | ----- | RRMSI | ----- | ----- | ----- |
YJL010C | 485 | RRRSV | RRRSI | RRRSI | RRRSF | RRRSL | ----- | ----- |
YJL005W/CYR1 | 57 | RRNSA | RRNSA | RRNSA | RRNSA | RRDSS | ----- | ----- |
YJL005W/CYR1 | 484 | RRSSI | RRTSI | RRTSI | RRTSI | ----- | ----- | ----- |
YJL005W/CYR1 | 1644 | RRSTF | RRNTF | RRTSF | RRSTF | RRTTF | ----- | ----- |
YJR001W/AVT1 | 47 | RRQSI | RRQSI | RRQSI | RRQSI | RRQSI | RRQSI | RRPSH |
YJR001W/AVT1 | 112 | RRLSV | RRLSV | RRLSV | RRLSV | RRLSV | RRLSV | ----- |
YJR049C/UTR1 | 64 | RRSSL | RRSSL | RRSSL | RRSSL | ----- | ----- | ----- |
YJR061W | 6 | RRFSM | RRFSM | RRFSM | RRFSM | ----- | ----- | ----- |
YJR066W/TOR1 | 854 | RRQTV | ----- | RRGTV | RRQTV | RRETV | RRETG | ----- |
YJR066W/TOR1 | 2253 | RRTTY | RRTTY | RRTTY | RRTTY | RRTTY | RRTTY | RRTTY |
YJR071W | 76 | RRTTV | ----- | ----- | ----- | ----- | ----- | ----- |
YJR092W/BUD4 | 592 | RRNTL | RRNTL | RRNTL | RRNTL | RRNTI | RRNTL | RRLSA |
YJR110W | 16 | RRGTA | RRGTA | RRGTA | RRGTA | ----- | ----- | ----- |
YJR119C | 337 | RRSSL | ----- | ----- | ----- | ----- | ----- | ----- |
YJR126C/VPS70 | 327 | RRDSV | ----- | ----- | ----- | ----- | ----- | ----- |
YJR127C/ZMS1 | 228 | RRHSV | RRHSV | RRHSV | RRHSM | ----- | ----- | ----- |
YJR127C/ZMS1 | 687 | RRDSV | RRDSV | RRDSV | RRDSV | RRKSS | ----- | ----- |
YJR134C/SGM1 | 580 | RRASV | RRASA | RRASF | RRASV | ----- | ----- | ----- |
YKL215C | 1192 | RRGSG | RRDSG | ----- | RRASG | ----- | ----- | ----- |
YKL203C/TOR2 | 863 | RRGTV | RRGTV | RRGTV | RRGTV | RRETV | RRETG | ----- |
YKL203C/TOR2 | 1245 | RRLSI | RRLSI | ----- | RRLSI | RRLSI | RRLSI | ----- |
YKL203C/TOR2 | 2256 | RRTTY | RRTTY | RRTTY | RRTTY | RRTTY | RRTTY | RRTTY |
YKL185W/ASH1 | 484 | RRSSG | RRSSG | RRSSG | RRSSG | ----- | ----- | ----- |
YKL180W/RPL17A | 126 | RRRTY | ----- | RRFSA | RRRTY | ----- | RRRTF | RRRTY |
YKL171W | 190 | RRDSF | RRDSF | RRDSF | RRNSF | ----- | ----- | ----- |
YKL171W | 240 | RRSSY | RRSSY | ----- | RRSSY | RRMSY | ----- | ----- |
YKL168C/KKQ8 | 71 | RRSTV | RRFTV | RRSTI | RRATI | ----- | ----- | ----- |
YKL150W/MCR1 | 69 | RRFTF | RRFTF | ----- | RRFTF | ----- | ----- | ----- |
YKL140W/TGL1 | 518 | RRLSA | RRLSA | RRLSA | RRLSA | ----- | ----- | ----- |
YKL118W | 17 | RRNSV | RRNSV | RRKSV | RRNSV | RRASL | ----- | ----- |
YKL097C | 93 | RRKTG | ----- | ----- | ----- | ----- | ----- | ----- |
YKL089W/MIF2 | 95 | RRSSL | RRSSL | RRSSL | RRSSL | ----- | ----- | ----- |
YKL088W | 39 | RRKSI | ----- | ----- | ----- | ----- | ----- | ----- |
YKL079W/SMY1 | 572 | RRGSM | ----- | ----- | ----- | ----- | ----- | ----- |
YKL078W/DHR2 | 552 | RRLSL | RRLSL | RRLSL | RRLSL | RRFTL | RRLSL | ----- |
YKL072W/STB6 | 511 | RRNSI | RRNSI | RRNSI | RRNSI | RRKSI | RRTSI | RRNSF |
YKL064W/MNR2 | 162 | RRKSV | RRKSV | RRKSV | RRKSV | ----- | ----- | ----- |
YKL064W/MNR2 | 618 | RRSSA | RRSSA | RRSSA | RRSSA | RRSSG | RRHSS | RRLSS |
YKL059C/MPE1 | 209 | RRTTG | RRTTG | ----- | RRTTG | ----- | RRTTG | ----- |
YKL058W/TOA2 | 11 | RRSTI | RRSTI | RRSTI | RRSTI | RRSTI | RRSTI | RRSTI |
YKL038W/RGT1 | 407 | RRQSA | RRQSV | ----- | RRQSA | ----- | ----- | ----- |
YKL038W/RGT1 | 477 | RRKSY | RRKSY | ----- | RRKSY | ----- | ----- | RRSSI |
YKL038W/RGT1 | 1127 | RRTSG | ----- | ----- | RRISN | ----- | ----- | ----- |
YKL020C/SPT23 | 223 | RRKSG | RRKSG | RRKSG | RRKSG | RRKSG | ----- | ----- |
YKL018C-A | 22 | RRETG | ----- | ----- | ----- | ----- | ----- | ----- |
YKR021W | 217 | RRGSV | RRGSV | RRGSV | RRGSV | RRKSA | ----- | ----- |
YKR031C/SPO14 | 283 | RRPSI | RRPSL | RRPSI | RRPSL | RRTSL | RRGSA | ----- |
YKR037C/SPC34 | 127 | RRRTA | RRKTT | RRKTV | RRKTM | ----- | RRKTN | ----- |
YKR037C/SPC34 | 196 | RRKTI | ----- | RRKTI | RRKTI | RRKTM | RRKTM | ----- |
YKR075C | 84 | RRWSI | RRWSI | RRWSI | RRWSI | RRWSI | RRWTI | ----- |
YKR077W | 289 | RRPSL | RRPSL | RRPSL | RRPSL | ----- | ----- | ----- |
YKR089C | 874 | RRHSI | RRHSV | RRHSV | RRHSV | RRNSA | RRTST | ----- |
YLL067C | 1017 | RRETI | ----- | ----- | ----- | ----- | ----- | ----- |
YLL066C | 981 | RRETI | ----- | ----- | ----- | ----- | ----- | ----- |
YLL054C | 303 | RRLSL | RRLSL | ----- | RRLSL | ----- | ----- | ----- |
YLL029W | 53 | RRASL | RRASL | RRASL | RRASL | RRAST | ----- | RRNST |
YLL021W/SPA2 | 1399 | RRMTI | RRMTI | RRMTI | RRMTL | RRMTV | RRMTI | ----- |
YLL018C/DPS1 | 543 | RRASL | RRASL | RRASL | RRASL | RRASL | RRASL | RRASL |
YLL016W/SDC25 | 231 | RRGTI | RRSTA | RRSTV | ----- | RRGSV | ----- | ----- |
YLL015W/BPT1 | 928 | RRASL | RRASL | RRASL | ----- | RRASD | RRASN | ----- |
YLL013C/PUF3 | 53 | RRSSF | RRSSF | RRSSF | RRSSF | RRSSF | RRPSI | ----- |
YLL005C/SPO75 | 300 | RRVSI | ----- | ----- | ----- | ----- | ----- | ----- |
YLR029C/RPL15A | 187 | RRKTW | RRKTW | RRKTW | RRKTW | ----- | RRKTW | RRHTW |
YLR047C | 211 | RRWSY | RRLSY | RRWSY | RRWSY | ----- | ----- | ----- |
YLR054C/OSW2 | 336 | RRFSI | RRFST | RRSFT | RRSFI | ----- | ----- | ----- |
YLR069C/MEF1 | 312 | RRSTI | RRATI | ----- | RRATI | RRATI | RRATI | RRSTI |
YLR082C/SRL2 | 306 | RRESI | ----- | RRESI | ----- | ----- | ----- | ----- |
YLR096W/KIN2 | 364 | RRATL | ----- | RRATL | RRATL | RRASL | ----- | ----- |
YLR096W/KIN2 | 823 | RRESL | ----- | RRESL | RRESL | RRESL | RRESL | ----- |
YLR106C/MDN1 | 1348 | RRLSV | ----- | RRLSV | RRLSV | ----- | ----- | ----- |
YLR112W | 9 | RRFSF | ----- | ----- | ----- | ----- | ----- | ----- |
YLR133W/CKI1 | 27 | RRHSL | RRPSL | RRHSL | RRHSL | RRPSL | RRPSL | RRPSL |
YLR133W/CKI1 | 82 | RRASA | ----- | ----- | ----- | ----- | ----- | ----- |
YLR141W/RRN5 | 105 | RRASA | RRASA | ----- | RRASA | RRNTI | ----- | ----- |
YLR149C | 480 | RRFSL | RRFSL | RRFSL | RRFSL | ----- | ----- | ----- |
YLR149C | 544 | RRCSF | RRCSF | RRCSF | RRCSF | RRAST | ----- | ----- |
YLR177W | 176 | RRSSY | RRSSY | RRSSY | RRSSY | RRSSY | RRSSY | RRPSQ |
YLR223C/IFH1 | 51 | RRFSL | RRFSL | RRFSL | ----- | ----- | RRFSL | ----- |
YLR223C/IFH1 | 1038 | RRQSM | RRQSM | RRQSM | RRQSM | RRQSI | RRQSI | RRASI |
YLR255C | 75 | RRRSY | ----- | ----- | ----- | ----- | ----- | ----- |
YLR272C/YCS4 | 415 | RRNSV | RRNSV | RRNSV | RRNSV | ----- | ----- | ----- |
YLR281C | 120 | RRRSV | RRRSI | RRRSV | ----- | ----- | ----- | ----- |
YLR299W/ECM38 | 91 | RRPSL | ----- | RRPTL | RRPSL | ----- | ----- | ----- |
YLR310C/CDC25 | 148 | RRSSL | RRSSL | RRSSL | RRSSL | RRGSA | RRNSF | ----- |
YLR342W/FKS1 | 817 | RRISF | RRISF | RRISF | RRISF | RRISF | RRITF | RRISF |
YLR342W/FKS1 | 1356 | RRYTL | RRYTL | RRYTL | RRYTL | RRYTL | ----- | RRYTL |
YLR345W | 236 | RRLSV | ----- | ----- | ----- | ----- | ----- | ----- |
YLR352W | 624 | RRNSL | ----- | ----- | ----- | ----- | ----- | ----- |
YLR359W/ADE13 | 334 | RRISL | RRISL | RRISL | RRISL | RRISL | RRISL | RRISL |
YLR363C/NMD4 | 51 | RRKSF | RRKSF | RRKSF | RRKSF | ----- | ----- | ----- |
YLR371W/ROM2 | 73 | RRRSG | ----- | ----- | ----- | ----- | ----- | ----- |
YLR371W/ROM2 | 393 | RRSSM | RRSSM | RRSSM | ----- | ----- | ----- | ----- |
YLR394W/CST9 | 30 | RRLSL | ----- | ----- | ----- | ----- | ----- | ----- |
YLR399C/BDF1 | 267 | RRSSA | ----- | RRSSA | RRSSA | ----- | RRRSD | ----- |
YLR403W/SFP1 | 102 | RRESI | RRESI | RRESI | RRESI | RRDSI | RRESI | RRDSV |
YLR403W/SFP1 | 133 | RRNSI | RRSSI | RRGSI | RRSSI | ----- | ----- | ----- |
YLR410W/VIP1 | 1118 | RRKSV | RRKSM | RRKST | RRKST | ----- | ----- | ----- |
YLR419W | 663 | RRISA | RRISA | RRISA | RRISA | RRISA | RRISA | RRLSA |
YLR466W/YRF1-4 | 1158 | RRETI | ----- | ----- | ----- | ----- | ----- | ----- |
YLR467W/YRF1-5 | 1572 | RRETI | ----- | ----- | ----- | ----- | ----- | ----- |
YML133C | 1150 | RRETI | ----- | ----- | ----- | ----- | ----- | ----- |
YML125C | 63 | RRRSL | RRRSL | RRRSL | RRRSL | ----- | RRKSV | ----- |
YML118W/NGL3 | 59 | RRNSL | RRNSL | RRNSL | ----- | ----- | ----- | ----- |
YML109W/ZDS2 | 180 | RRLSG | Rrlsg | Rrlsg | Rrlsg | ----- | ----- | ----- |
YML102W/CAC2 | 261 | RRCSI | RRCSI | RRCSI | RRCSI | RRLTI | ----- | ----- |
YML059C | 1675 | RRNSI | ----- | ----- | ----- | ----- | ----- | ----- |
YML016C/PPZ1 | 119 | RRSSY | RRSSY | RRSSY | RRSSY | ----- | ----- | ----- |
YML010W/SPT5 | 438 | RRPTF | RRPTF | RRPTF | RRPTF | RRATY | ----- | ----- |
YMR005W/MPT1 | 371 | RRNSV | ----- | ----- | ----- | ----- | ----- | ----- |
YMR037C/MSN2 | 579 | RRKSA | RRKSA | RRKSA | RRKSA | RRKSS | ----- | RRKSS |
YMR037C/MSN2 | 617 | RRPSY | RRPSY | ----- | RRPSY | ----- | ----- | ----- |
YMR037C/MSN2 | 622 | RRKSM | RRKSM | RRKSM | RRKSM | ----- | RRKSS | ----- |
YMR037C/MSN2 | 630 | RRSSV | RRSSV | RRSSY | RRSSV | RRASV | RRSSR | ----- |
YMR053C/STB2 | 591 | RRNSF | RRNSF | RRNSF | RRNSF | RRNSL | RRTSI | RRNSF |
YMR057C | 106 | RRVSF | ----- | ----- | ----- | ----- | ----- | ----- |
YMR058W/FET3 | 456 | RRDTL | RRDTL | ----- | RRDTL | ----- | ----- | ----- |
YMR068W/AVO2 | 270 | RRSSI | RRSSI | RRGSI | RRSSI | ----- | ----- | ----- |
YMR068W/AVO2 | 404 | RRVSL | RRVSL | RRVSL | RRVSL | RRLSL | ----- | ----- |
YMR076C/PDS5 | 375 | RRTSV | RRSSV | RRASV | RRASV | ----- | ----- | ----- |
YMR081C/ISF1 | 262 | RRESF | RRESS | RRGSS | RRASS | ----- | ----- | ----- |
YMR081C/ISF1 | 323 | RRKSF | ----- | RRKSF | RRKSF | RRKSS | ----- | ----- |
YMR086W | 4 | RRTTA | RRTTA | RKTTA | RRTTA | ----- | RKTTA | ----- |
YMR086W | 631 | RRASL | RRTSV | RRTSV | RRNSV | ----- | RRTSV | ----- |
YMR100W/MUB1 | 445 | RRWSY | ----- | ----- | ----- | ----- | ----- | ----- |
YMR107W | 57 | RRSSI | RRSSM | RRSSM | RRSSM | ----- | ----- | ----- |
YMR121C/RPL15B | 187 | RRKTW | RRKTW | RRKTW | ----- | ----- | RRKTW | RRHTW |
YMR155W | 540 | RRATA | RRTTG | ----- | RRMTT | ----- | ----- | ----- |
YMR162C/DNF3 | 95 | RRRTF | RRRTF | RRRTF | RRRTF | ----- | ----- | ----- |
YMR172W/HOT1 | 583 | RRRSL | ----- | ----- | ----- | ----- | ----- | ----- |
YMR179W/SPT21 | 414 | RRSSV | ----- | RRSSI | RRSSL | ----- | ----- | ----- |
YMR184W | 76 | RRPSV | ----- | ----- | ----- | ----- | ----- | ----- |
YMR190C/SGS1 | 761 | RRQTF | RRQTF | ----- | RRQTF | ----- | RRQTF | ----- |
YMR191W | 62 | RRASG | ----- | ----- | ----- | ----- | ----- | ----- |
YMR196W | 978 | RRKSA | RRKSA | ----- | RRKSA | RRKSA | RRKSA | RRKSG |
YMR206W | 295 | RRNSI | RRNSI | RRNSI | RRNSI | RRNSI | ----- | ----- |
YMR210W | 132 | RRYSY | ----- | RRYSY | RRYSY | ----- | ----- | ----- |
YMR216C/SKY1 | 380 | RRHTI | RRHTI | RRHTI | RRHTI | RRHTI | RRHTI | ----- |
YMR220W/ERG8 | 138 | RRLSF | ----- | RRLSF | RRLSF | ----- | ----- | ----- |
YMR221C | 266 | RRKSV | RRKSV | RRKSV | RRKSV | RRKSV | RRKSV | ----- |
YMR237W | 626 | RRDSV | RRDSV | ----- | RRDSV | ----- | ----- | ----- |
YMR243C/ZRC1 | 384 | RRFSI | RRFSI | RRFSI | RRFSI | ----- | RRFSR | ----- |
YMR265C | 104 | RRYSW | RRFSW | RRFSW | ----- | ----- | ----- | ----- |
YMR275C/BUL1 | 104 | RRSTA | RRATA | RRSTA | RRSTA | ----- | ----- | ----- |
YMR284W/YKU70 | 190 | RRLTI | RRLTI | ----- | RRLTV | ----- | ----- | ----- |
YMR288W/HSH155 | 181 | RRTSM | RRTSM | RRTSM | RRTSM | ----- | ----- | ----- |
YMR292W/GOT1 | 64 | RRGSL | RRGSL | RRGSL | RRGSL | RRGSI | RRGSL | ----- |
YMR306W/FKS3 | 703 | RRISF | RRISF | RRISF | RRISF | RRISF | RRITF | RRISF |
YNL339C/YRF1-6 | 1636 | RRETI | ----- | RRETI | RRETI | ----- | ----- | ----- |
YNL262W/POL2 | 1124 | RRWTL | RRWTL | RRWTL | RRWTL | RRWTL | ----- | ----- |
YNL247W | 9 | RRYTI | ----- | ----- | RRYSI | ----- | ----- | ----- |
YNL221C/POP1 | 97 | RRRTA | RRRTA | RRRTA | RRRTA | RRRTA | RRTTA | ----- |
YNL197C/WHI3 | 565 | RRLSF | RRLSF | RRLSF | RRLSF | RRLSF | RRLSF | RRLSF |
YNL186W/UBP10 | 582 | RRFSI | RRFSI | RRFSI | RRFSI | ----- | RRYSI | ----- |
YNL169C/PSD1 | 53 | RRSSG | RRSSG | RRSSG | RRNSG | ----- | ----- | ----- |
YNL154C/YCK2 | 165 | RRFSV | RRFSV | RRFSV | RRFSV | RRFSV | ----- | RRFSV |
YNL121C/TOM70 | 171 | RRASA | RRASA | RRASA | RRASA | RRASA | RRASA | ----- |
YNL106C/INP52 | 33 | RRSTC | ----- | ----- | RRSTC | ----- | ----- | ----- |
YNL103W/MET4 | 203 | RRNTF | RRNTF | RRNTF | RRNTF | ----- | ----- | ----- |
YNL099C/OCA1 | 198 | RRFTG | RRFTG | RRFTG | RRFTG | RRFTG | RRFTG | RRFTG |
YNL092W | 362 | RRESG | ----- | ----- | ----- | ----- | ----- | ----- |
YNL087W | 578 | RRKTI | RRKTI | RRKTI | RRKTI | ----- | ----- | ----- |
YNL076W/MKS1 | 515 | RRQSM | RRESM | RRESM | RRESM | RRESM | RRESM | ----- |
YNL074C/MLF3 | 118 | RRRTL | RRRTL | RRRTL | RRRTL | ----- | ----- | ----- |
YNL058C | 120 | RRFTM | ----- | RRSTI | RRSSI | ----- | ----- | ----- |
YNL054W/VAC7 | 686 | RRYSG | RRYSG | RRYSG | RRYSG | RRYSG | RRYSG | ----- |
YNL007C/SIS1 | 245 | RRKTL | RRKTL | RRKTL | RRKTL | ----- | ----- | ----- |
YNL005C/MRP7 | 231 | RRESW | ----- | ----- | ----- | ----- | ----- | ----- |
YNR014W | 112 | RRNSV | RRNSV | RRNSV | RRNSV | ----- | ----- | ----- |
YNR014W | 194 | RRNSV | RRNSV | RRNSV | RRNSV | RRNSV | ----- | ----- |
YNR016C/ACC1 | 1259 | RRITF | RRITF | ----- | RRITF | RRITF | ----- | RRITF |
YNR039C/ZRG17 | 110 | RRQSL | RRQSL | RRQSL | RRQSL | RRHSL | RRHSF | ----- |
YNR056C/BIO5 | 504 | RRMSM | RRMSI | ----- | ----- | ----- | ----- | ----- |
YOL164W | 516 | RRSTG | ----- | ----- | ----- | ----- | ----- | ----- |
YOL146W/PSF3 | 36 | RRASI | ----- | ----- | ----- | ----- | ----- | ----- |
YOL130W/ALR1 | 612 | RRKTM | RRKTM | RRKTM | RRKTM | RRKTM | RRKTM | ----- |
YOL116W/MSN1 | 28 | RRMSM | RRMSM | RRMSM | RRMSM | ----- | ----- | ----- |
YOL100W/PKH2 | 430 | RRLTI | ----- | ----- | ----- | ----- | ----- | RRATI |
YOL081W/IRA2 | 1742 | RRNSC | RRNSC | RRNSC | RRNSC | RRNSP | ----- | RRNST |
YOL078W/AVO1 | 531 | RRDTV | RRDTV | RRDTV | RRDTA | ----- | ----- | ----- |
YOL075C | 192 | RRLSI | RRVSI | RRLSI | RRLSI | RRLSV | RRVSI | RRVSL |
YOL075C | 845 | RRVTM | RRVSI | RRVSI | RRVSI | RRVTM | RRVSL | RRVSI |
YOL038W/PRE6 | 48 | RRSTL | RRSTL | RRSTL | RRSTL | RRSTL | ----- | ----- |
YOL017W/ESC8 | 614 | RRTTV | ----- | ----- | ----- | ----- | ----- | ----- |
YOL015W | 491 | RRSSV | ----- | ----- | ----- | ----- | ----- | ----- |
YOR002W/ALG6 | 468 | RRRSL | RRRSL | RRRSL | RRRSL | ----- | RRRSL | ----- |
YOR017W/PET127 | 11 | RRLSV | RRLSA | ----- | ----- | ----- | ----- | ----- |
YOR018W/ROD1 | 614 | RRNSY | RRNSY | RRNSY | RRNSY | ----- | ----- | ----- |
YOR028C/CIN5 | 96 | RRNSV | RRNSV | RRNSV | RRNSV | ----- | ----- | ----- |
YOR028C/CIN5 | 207 | RRVSV | RRVSV | RRVSV | RRDSI | ----- | ----- | ----- |
YOR040W/GLO4 | 36 | RRNSW | ----- | ----- | ----- | ----- | ----- | ----- |
YOR062C | 84 | RRWSI | RRWSI | RRWSV | RRWSI | RRWSI | RRWTI | ----- |
YOR066W | 58 | RRLSI | RRLSV | RRPSI | RRLSI | ----- | ----- | ----- |
YOR073W | 193 | RRQSM | RRQSM | RRQSM | RRQSM | RRQSM | RRESL | ----- |
YOR083W/WHI5 | 167 | RRSTG | ----- | ----- | ----- | ----- | ----- | ----- |
YOR092W/ECM3 | 309 | RRNSI | RRNSI | RRNSV | RRNSI | ----- | RRGSI | RRTSN |
YOR093C | 87 | RRHSI | RRHSI | RRHSI | RRHSI | ----- | ----- | ----- |
YOR102W | 4 | RRQTL | ----- | ----- | ----- | ----- | ----- | ----- |
YOR113W/AZF1 | 300 | RRNSF | RRNSS | RRNSS | RRNSS | RRNSS | RRDSS | ----- |
YOR129C | 730 | RRRTY | ----- | ----- | ----- | ----- | ----- | ----- |
YOR133W/EFT1 | 710 | RRATY | RRATY | RRATY | RRATY | RRATY | RRATY | RRVTY |
YOR151C/RPB2 | 336 | RRGTA | RRGTA | RRGTA | RRGTA | RRGTA | ----- | ----- |
YOR188W/MSB1 | 535 | RRDSA | ----- | ----- | ----- | ----- | ----- | ----- |
YOR196C/LIP5 | 295 | RRATY | RRATY | ----- | RRATY | ----- | RRATY | RRATY |
YOR209C/NPT1 | 181 | RRRSL | RRRSF | ----- | RRRSL | RRRSF | ----- | RRRSY |
YOR211C/MGM1 | 845 | RRLTL | RRLTL | RRLTL | RRLTL | ----- | ----- | ----- |
YOR219C/STE13 | 81 | RRESF | ----- | ----- | ----- | ----- | ----- | ----- |
YOR230W/WTM1 | 403 | RRNTA | ----- | RRNTA | RRNTA | ----- | ----- | ----- |
YOR233W/KIN4 | 83 | RRDTI | RRDTI | RRDSI | RRDTI | RRDTI | RRDTV | ----- |
YOR242C/SSP2 | 29 | RRSSF | ----- | RRSSF | RRSSF | ----- | ----- | ----- |
YOR254C/SEC63 | 312 | RRDSG | ----- | RRDSG | RRDSG | ----- | ----- | ----- |
YOR254C/SEC63 | 509 | RRGSW | ----- | RRGSW | RRGSW | ----- | ----- | ----- |
YOR264W/DSE3 | 133 | RRPTL | ----- | ----- | ----- | ----- | ----- | ----- |
YOR291W | 163 | RRSSI | RRSSM | RRSSM | RRSSM | RRSSI | ----- | ----- |
YOR291W | 939 | RRMSV | RRMSV | RRMSV | RRMSV | RRMSV | RRMSV | ----- |
YOR306C/MCH5 | 231 | RRGTA | RRGTA | ----- | RRGTA | RRGTA | ----- | ----- |
YOR307C/SLY41 | 11 | RRNSV | RRNSV | RRNSV | RRNSV | RRNSV | RRASI | ----- |
YOR317W/FAA1 | 211 | RRQSG | ----- | ----- | ----- | ----- | ----- | ----- |
YOR324C/FRT1 | 198 | RRGSM | ----- | RRGSM | RRGSM | ----- | ----- | ----- |
YOR324C/FRT1 | 225 | RRSSA | RRSSA | RRSSA | RRSSA | ----- | ----- | ----- |
YOR347C/PYK2 | 21 | RRTSI | RRTSI | RRTSI | RRTSI | RRTSI | RRTSI | RRSSI |
YOR352W | 128 | RRLSM | RRLSM | RRLSM | RRLSM | RRLSM | RRLSM | ----- |
YOR370C/MRS6 | 6 | RRPSM | RRPSM | RRPSM | RRPSM | RRPSM | RRPSM | RRKSM |
YOR370C/MRS6 | 13 | RRPSF | RRPSF | RRPSF | RRPSF | RRPSI | RRPSF | RRPST |
YOR396W | 1000 | RRETI | ----- | ----- | ----- | ----- | ----- | ----- |
YPL283C/YRF1-7 | 1636 | RRETI | ----- | ----- | ----- | ----- | ----- | ----- |
YPL260W | 366 | RRWTL | RRWTL | RRWTL | RRWTL | RRWTL | ----- | RRWTL |
YPL230W | 37 | RRKTF | RRKTF | RRKTF | RRKSF | RRKTF | ----- | ----- |
YPL219W/PCL8 | 295 | RRISL | RRISL | RRISL | RRISL | ----- | RRPSV | ----- |
YPL217C/BMS1 | 113 | RRLTF | RRLTF | RRLTF | RRLTF | RRLTM | RRLTF | RRLTF |
YPL209C/IPL1 | 257 | RRKTV | RRKTV | ----- | RRKTV | ----- | ----- | ----- |
YPL204W/HRR25 | 98 | RRFSF | RRFSF | RRFSF | RRFSF | ----- | ----- | ----- |
YPL191C | 340 | RRSSL | ----- | ----- | RRNSL | ----- | ----- | ----- |
YPL190C/NAB3 | 171 | RRETL | ----- | ----- | RRETL | ----- | ----- | ----- |
YPL190C/NAB3 | 448 | RRHTV | ----- | RRHTV | RRHTV | ----- | ----- | ----- |
YPL181W/CTI6 | 213 | RRNSM | RRNST | RRNST | RRNSI | ----- | RRTSH | ----- |
YPL181W/CTI6 | 431 | RRVSA | RRVSA | RRVSA | RRVSA | ----- | ----- | ----- |
YPL162C | 75 | RRRSL | ----- | ----- | ----- | ----- | ----- | ----- |
YPL150W | 127 | RRISL | ----- | ----- | RRLSL | ----- | ----- | ----- |
YPL118W/MRP51 | 324 | RRPSV | RRPSV | RRPSV | RRPSV | RRSSS | ----- | ----- |
YPL100W/ATG21 | 234 | RRNSL | RRNSL | ----- | RRNSL | RRNSV | ----- | ----- |
YPL094C/SEC62 | 183 | RRGSY | RRGSY | RRGSY | RRGSY | RRGSY | ----- | ----- |
YPL085W/SEC16 | 1608 | RRESI | RRESI | RRESI | RRESI | RRSSA | ----- | ----- |
YPL077C | 92 | RRNSV | RRNSV | RRNSV | RRNSV | ----- | ----- | ----- |
YPL064C | 95 | RRNTW | ----- | ----- | ----- | ----- | ----- | ----- |
YPL018W/CTF19 | 67 | RRNTL | ----- | RRNTL | RRNTL | ----- | ----- | ----- |
YPL011C/TAF47 | 343 | RRTSL | RRTSL | RRTSL | RRTSL | RRESI | RRESF | ----- |
YPR010C/RPA135 | 1129 | RRCSM | RRCSM | RRCSM | RRCSM | ----- | ----- | ----- |
YPR032W/SRO7 | 599 | RRFSL | RRFSL | RRFSL | RRFSL | RRFSL | RRFSL | ----- |
YPR049C/CVT9 | 469 | RRLSW | RRLSW | RRLSW | RRLSW | ----- | ----- | ----- |
YPR074C/TKL1 | 332 | RRLSG | RRLSG | RRLSG | RRLSG | ----- | RRLSG | ----- |
YPR131C/NAT3 | 88 | RRISL | RRISL | RRISL | RRISL | RRISL | RRISL | ----- |
YPR138C/MEP3 | 44 | RRKSA | RRKSA | RRKSA | RRKSA | RRKSA | RRKSA | RRKSA |
YPR149W/NCE102 | 159 | RRRTG | RRRTG | RRRTG | RRRTG | ----- | ----- | ----- |
YPR160W/GPH1 | 28 | RRLTG | RRLTG | RRLTG | RRLTG | RRLTG | RRLTG | ----- |
YPR170C | 40 | RRGSC | ----- | ----- | ----- | ----- | ----- | ----- |
YPR185W/APG13 | 578 | RRNSL | RRNSL | RRNSL | RRNSL | RRNSL | RRNSL | RRSSN |
YPR187W/RPO26 | 65 | RRKTL | RRKTL | RRKTL | RRKTL | RRKTL | RRRTL | ----- |
YPR191W/QCR2 | 15 | RRLTV | RRLTV | RRLTV | RRLTV | ----- | ----- | ----- |
YPR204W | 809 | RRETI | ----- | ----- | ----- | ----- | ----- | ----- |
The degree to which each of these sites is conserved among the six budding yeast species analyzed in this study is shown. The sites conserved out to Candida albicans (C. alb.) are highlighted in yellow. S. cer., S. cerevisiae; S. bay., S. bayanus; S. kud., S. kudriavzevii; S. mik., S. mikatae; S. cas., S. castelli.
Table 3. The 85 candidate PKA substrates identified by the evolutionary proteomics approach described here
Biological process | Gene name |
Protein synthesis (12) | BMS1, DPS1, EFT1, EFT2, IFH1, MEF1, RPL15A, RPL15B, RPL17A, RPS8A, RPS8B, SUP35 |
Transport (12) | ADP1, ATG1, ATG13, ATG18, AVT1, MEP1, MEP3, MNR2, MRS6, SEC53, TOM71, YOL075c |
Signal transduction (9) | BCY1, BMH1, BMH2, IRA1, IRA2, SYG1, TOR1, TOR2, YAK1 |
RNA pol II transcription (6) | ACA1, CST6, HIR1,STP1, STP2 |
Cell wall synthesis (5) | ECM3, FKS1, GCS1, ROT2, SSD1 |
Chromatin assembly (4) | NPT1, STB2, STB3, STB6 |
Lipid biosynthesis (4) | ACC1, CKI1, LIP5, SCT1 |
Cell size regulation (3) | SFP1, WHI3, WHI4 |
Nucleotide biosynthesis (3) | ADE13, PRS2, PRS3 |
Stress response (3) | MSN2, OCA1, RIM15 |
Glycolysis (2) | CDC19, PYK2 |
Spindle assembly (2) | DAM1, SPC105 |
Bud site selection (1) | BUD4 |
Cell morphogenesis (1) | YCK2 |
Protein degradation (1) | RSP5 |
RNA pol III transcription (1) | MAF1 |
Splicing (1) | PRP8 |
Sporulation (1) | MDS3 |
Unknown (14) | FKS3, IKS1, PSP1, YBL029w, YBR028c, YBR220c, YER049c, YHR032w, YJL046w, YLL029w, YLR177w, YLR419w, YMR196w, YPL260w |