Supporting information for Levenberg et al. (March 26, 2002) Proc. Natl. Acad. Sci. USA, 10.1073/pnas.032074999.

RT and activation: 30°C for 30 min, 95°C for 15 min.

Amplification: 94°C for 1 min, 55°C for 1 min, 72°C for 1 min. See below for cycle numbers.

Final extension: 72°C for 10 min.

Optimal cycle numbers were determined for each gene to ensure that conditions were in the linear range of PCR amplification:

PECAM1: 28 cycles

VE-cad: 32 cycles

CD34: 30 cycles

Flk-1: 31 cycles

Tie-2: 31 cycles

GATA-2: 33 cycles

GATA-3: 29 cycles

AC133: 27 cycles

OCT-4: 31 cycles

GAPDH: 24 cycles

PECAM1: GCTGTTGGTGGAAGGAGTGC / GAAGTTGGCTGGAGGTGCTC

VE-cad: CCGGCGCCAAAAGAGAGA / CTGGTTTTCCTTCAGCTGGAAGTGGT

CD34: TGAAGCCTAGCCTGTCACCT / CGCACAGCTGGAGGTCTTAT

Flk-1: CAACAAAGCGGAGAGGAG / ATGACGATGGACAAGTACCC

Tie-2: CCTTAGTGACATTCTTCC / GCAAAAATGTCCACCTGG

GATA-2: CCCTAAGCAGCGCAGCAAGAC / TGACTTCTCCTGCATGCACT

GATA-3: ACCCCACTGTGGCGGCGAGAT / CACAGCACTAGAGACC

AC133: CAGTCTGACCAGCGTGAAAA / GGCCATCCAAATCTGTCCTA

OCT-4: GAGAACAATGAGAACCTTCAGGAGA / TTCTGGCGCCGGTTACAGAACCA

GAPDH: AGCCACATCGCTCAGACACC / GTACTCAGCGGCCAGCATCG