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Fig. 9. CD19 and sIgG1 expression during BCR and CD38 ligation-induced B cell activation. B cells were cultured for 5 days in the presence and absence of anti-m plus anti-CD38 (1 mg/ml each) and RA (10 nM). At the time of harvesting each day, surface CD19 and IgG1 were detected by anti-CD19-PE and anti-IgG1-Alexa 488 antibodies, respectively, and analyzed by flow cytometry. Representative flow cytometric graphs show the percentage of B cells with CD19 and IgG1 staining harvested on days 1, 3, and 5.