Genome expression analysis of Anopheles gambiae: Responses to injury, bacterial challenge, and malaria infection -- Data Supplement - Supporting Information -- Proceedings of the National Academy of Sciences

Supporting information for Dimopoulos et al. (2002) Proc. Natl. Acad. Sci. USA, 10.1073/pnas.092274999.

Fig. 5.
Extension of Fig. 3. In vivo responses to sterile (STE) and septic (SEP) injury of adult mosquitoes and cross-correlation with in vitro data from the cell line. The expression profiles (1, 6, 12, and 24 hrs) of genes showing at least 2-fold regulation in at least two of the eight STE and SEP experiments, or at least 2-fold regulation after malaria (M) challenge, were SOM-sorted into three clusters. The respective profiles from the cell-line challenge experiments (see Figs. 2 and 4) were then added, after averaging the responses to six different bacteria (BACT). The combined expression profiles were again SOM-sorted. Limited manual repositioning of genes within the clusters helped to highlight the patterns, including the existence of subclusters. The profiles of genes that were regulated after malaria challenge were used to construct the malaria-specific cluster, which was analyzed by hierarchical clustering (bottom cluster).