Positive selection of self-MHC-reactive T cells by individual peptide-MHC class II complexes -- Data Supplement - Supporting Figure 6 -- Proceedings of the National Academy of Sciences

Supporting information for Barton et al. (2002) Proc. Natl. Acad. Sci. USA 99 (10), 6937–6942. (10.1073/pnas.102645699)

Fig. 6.
The overexpressed peptide-MHC class II complexes are responsible for the observed differences in self-MHC reactivity of CD4 T cells. Proliferative responses of splenocytes from EaIi^+/–H-2M⁰ [Ea ´ CLIP (class II-associated invariant chain peptide)] mice treated with YAe, a 1:1 mixture of 15G4 and 30-2 antibodies, or normal mouse IgG as a control. Splenic T cells were stimulated with irradiated syngeneic splenocytes (open bars) or C57BL/6 splenocytes (closed bars). Cultures were incubated for 96 h. Proliferation was measured by [³H]thymidine incorporation during the last 24 h of culture. The data are presented as the mean cpm of triplicate cultures.