Previously uncharacterized genes in the UV- and MMS-induced DNA damage response in yeast -- Data Supplement - Supporting Information -- Proceedings of the National Academy of Sciences

Supporting information for Hanway et al. (2002) Proc. Natl. Acad. Sci. USA, 10.1073/pnas.152264899

Table 6. Growth response of MMS-treated strains as determined by competition assays

ORF	RF 0.001%^4pts		RF 0.01%^4pts
High sensitivity
COQ4*	0.72		0.70
COX7	0.82		0.78
COX18	0.87		0.85
ESC4*^†‡	0.69		0.64
HAP2	0.85		0.85
HOM3*	0.64		0.63
IKI3	0.88		0.73
IRA2*	0.83		0.79
ISA1	0.83		0.88
ISM1	0.85		0.84
MMS2^§	0.85		0.70
MRPL7	0.78		.087
MUS81^§	0.85		0.79
PET309	0.84		0.79
RAD5^§	0.68		0.66
RSM22	0.66		0.63
TIM13*^†‡	0.85		0.76
YCL061C*	0.72		0.69
YER049W*	0.71		0.68
YER084W	0.85		0.86
YER097W	0.85		0.85
YGR228W	0.88		0.81
YHR207C	0.88		0.84
YLR368C	0.88		0.87
YLR376C*^†‡	0.82		0.59
YOR006C	0.83		0.87
YOR338W	0.88		0.84
YPL098C	0.74		0.74
Moderate sensitivity
CKB2	1.00			0.79
CEM1	0.87		1.01
CTR1	1.02		0.64
DOA1	0.98		0.69
DRS2	1.00		0.84
ELA1	0.81		0.95
GBP2	0.84		0.96
HOS2	0.88		0.93
ILV1	0.99		0.83
ISC1	0.89		0.86
KRE11	0.86		1.00
LST4	0.98		0.74
MAC1*	0.94		0.73
MEC3^§	0.95		0.83
MRPL33	0.80		0.90
MSS1	0.79		0.91
MSY1	0.88		1.05
PDB1	0.80		0.91
PHB1	0.89		0.88
POS5	0.92		0.88
PPH3*	0.99		0.67
RAD14^§	0.89		0.73
RAD17^§	1.02		0.88
REV1^§	1.06		0.84
RPL34A	0.86		0.98
SAP155	0.76		1.03
SBA1	0.87		0.94
SHU1	0.97		0.69
SLT2	0.92		0.87
TOF1	0.97		0.81
TOS10	0.97		0.87
TRP3	0.98		0.73
VPS29	0.66		1.06
YAL011W	0.99		0.84
YBR223C*	0.89		0.70
YDR078C	0.93		0.69
YDR338C	0.84		0.97
YER028C	0.86		0.89
YER050C	0.85		1.04
YER093C-A	0.94		0.88
YER083C	0.98		0.76
YGR136W*	0.89		0.67
YGR138C	0.87		0.90
YGR182C	0.96		0.82
YHR045W	0.97		0.63
YKL098W*	0.98		0.62
YKR021W	0.80		0.89
YKR033C	075		0.93
YLR135W	1.04		0.65
YLR374C	0.80		1.06
YMR030W	0.84		0.94
YNL201C	1.00		0.60
YOR284W	0.91		0.84
YPL170W	1.05		0.78
YPR123C	1.04		0.66
YPR153W	1.03		0.76
Low sensitivity
ADE6	0.89		1.06
APA1	0.92		0.95
AXL1	0.89		0.90
BNI4	0.89		0.94
BUD9	0.92		0.95
CDC50^§	0.96		0.91
CLU1	0.90		0.99
DIT2	0.91		0.99
EGD2	0.90		0.97
FMS1	0.92		1.01
FPS1	0.91		0.89
FTH1	0.90		0.97
GLN3	0.92		1.04
HVG1	0.91		0.96
HXT2	0.90		0.99
MDJ2	0.89		1.00
PCD1	0.97		0.91
PET111	0.99		0.89
PHR1^§	0.89		0.91
PPT1	0.90		0.98
QRI8	0.89		1.00
RAD9^§	0.99		0.90
REV3^§	1.07		0.89
RIM101	0.97		0.92
RTG2	0.95		0.91
SCS7	0.95		0.89
SLG1	0.90		0.95
SNF7	0.94		0.92
SPO7	0.97		0.91
STB4	0.91		1.00
STF1	0.90		0.93
STP22	0.94		0.89
SYF2	0.89		0.97
TOM6	0.93		0.90
UBI4	1.02		0.91
URE2	0.89		1.01
VPS36	0.92		0.90
YBL065W	0.91		0.91
YCR007C	0.91		0.94
YDR132C	0.90		0.93
YDR540C	0.97		0.92
YEL064C	0.90		0.97
YER041W	0.88		0.94
YFR056C	0.90		0.98
YGR066W	0.89		0.90
YGR122W	0.95		0.92
YGR206W	0.89		0.89
YHL005C	0.98		0.92
YHR189W	0.99		0.92
YLL029W	0.89		0.91
YLR239C	0.92		0.89
YLR401C	0.90		0.98
YLR412W	0.99		0.91
YLR415C	0.92		0.96
YMR007W	0.92		0.99
YMR018W	0.92		1.01
YMR034C	0.92		0.99
YMR163C	0.89		0.97
YMR251W	0.91		0.98
YOR192C	0.93		0.92
YPL047W	1.00		0.90
YPL184C		0.95			0.89
YPR126C		0.89			0.91

Relative fitness ratios (RF) from samples treated with 0.001% or 0.01% methyl methanesulfonate (MMS). RF^4pts values are determined by calculating the growth rate of the strains using samples taken after 7, 13, and 20 generations of growth and computing the ratio of the growth rates in treated versus untreated samples. High sensitivity, both relative fitness ratios below 0.88; moderate sensitivity, one ratio below 0.88; low sensitivity, one ratio below 0.92.

*Strains chosen for individual analysis.

^†Strains displaying increased sensitivity during individual treatment. ^‡Successfully complemented strains. ^¶Previously implicated in DNA damage repair, or known to be MMS sensitive.