Supporting information for Trinh et al. (2002) Proc. Natl. Acad. Sci. USA, 10.1073/pnas.152337399
Table 2. Design strategies for preventing aggregation of b strand edges
MBb2m | HLAb2m | MHb2m | |
Continuous H-bonding | N | N | Y |
Covering loop | N | N | N |
b bulge | Y | Y | N |
Proline | N | N | N |
Inward-pointing charge (strand D) | Y | Y | N |
Sheet edge rolls in | N | N | N |
Very short | Y | Y | N |
Very twisted | Y | Y | N |
LbGly bend, reverse twist | N | N | N |
Switch between sheets | N | N | N |
The criteria were taken from ref. 1. The table depicts whether the different structures of b2m contain these design features in edge strand D (residues 50--57). HLAb2m was taken from Protein Data Bank (PDB) ID code 1DUZ (2), MBb2m was taken from PDB ID code 1BMG (3). MHb2m was taken from the crystal structure presented here. (MBb2m, monomeric bovine b2m; MHb2m, monomeric human b2m; HLAb2m, human b2m bound to the HLA class 1 complex.)
1. Richardson, J. S. & Richardson, D. C. (2002) Proc. Natl. Acad. Sci. USA 99, 2754–2759.
2. Khan, A. R., Baker, B. M., Ghosh, P., Biddison, W. E. & Wiley, D. C. (2000) J. Immunol. 164, 6398–6405.
3. Becker, J. W. & Reeke, G. N. (1985) Proc. Natl. Acad. Sci. USA 82, 4225–4229.