Supporting information for Geiss et al. (2002) Proc. Natl. Acad. Sci. USA, 10.1073/pnas.112338099
Fig. 10.
Hierarchical clustering results for genes known to be regulated by NF-k B. A set of genes with known NF-k B binding sites was created in RESOLVER gene expression data analysis software (Rosetta Biosoftware, Kirkland WA) based on a recent view of the subject [Pahl, H. L. (1999) Oncogene 18, 6853–6866]. There were 139 genes in the original set. Genes that were significantly regulated (³ 2 fold, £ 0.01 P value) in at least one experiment are shown. Genes were clustered with agglomerative algorithm, complete link heuristic criteria, and correlation without mean subtraction metric. Experiments were not clustered. The fold change in mRNA levels in influenza virus-infected cells relative to mock-infected cells is represented by red (increased in influenza virus-infected cells) or green (decreased in influenza virus-infected cells) squares. Each square is the result of four measurements per gene per experiment. The color scale indicates the magnitude of increase or decrease in log10 scale. Gray indicates gene was not present on the array used in those experiments. Each vertical column represents an independent infection. From left to right, A549 cells infected with delNS1 virus, cells infected with NS1(1-126) virus, cells infected with wt A/PR/8/34 virus, cells infected with wt A/WSN/33 virus, and cells infected with 1918 NS recombinant virus. Characterized genes are indicated by their HUGO names, and more information and aliases for these genes can be found at GeneCards database (http://bioinfo.weizmann.ac.il/cards/index.html).