Supporting information for Giannakakou et al. (2002) Proc. Natl. Acad. Sci. USA, 10.1073/pnas.132275599.
Fig. 7.
Treatment of A549 cells with 1 nM PTX or nocodazole (Noc) enhances Ad2-TR nuclear targeting. A549 cells were pretreated with 1 nM PTX or Noc for 18 hr before infection with Ad2-TR. Representative samples from untreated (no drug) or treated cells are shown at 15 min postinfection. The intracellular distribution of Ad2-TR viral particles is shown (Left) (a, d, and g), 4',6-diamidino-2-phenylindole (DAPI) staining of the nucleus (b, e, h), and differential interference contrast microscopy images (c, f, i) are shown (Center and Right, respectively. (Bar = 20 mm.)